JP6964871B2 - Range of motion improver - Google Patents

Description

The present invention comprises a range of motion improving agent and a food composition for improving the range of motion containing collagen, no grape and N-acetylglucosamine as active ingredients, and a locomotive syndrome preventing or improving agent and locomotive containing these. Concerning food compositions for preventing or improving syndrome.

Locomotive syndrome (locomotive syndrome, locomotive syndrome) refers to a state in which daily life is restricted due to a disorder of the locomotive organ, and the risk of requiring long-term care or becoming bedridden increases (Non-Patent Document 1). Currently, 47 million people including the reserve army are said to be applicable, and it can be said that it is one of the national diseases. The locomotive organs include muscles, bones, joints, ligaments, tendons, etc., and the main symptoms of locomotive syndrome include reduction / decrease in range of motion, decrease in muscle strength and balance, and joint pain. These symptoms are complicatedly related to multiple locomotor organs, and the current situation is that preventive methods and improvement methods are not progressing.

The range of motion of a joint is determined by how tightly multiple musculoskeletal organs such as muscles, ligaments, and joint capsules surround the joint. (Patent Document 1). When the range of motion of joints is reduced or decreased due to lack of exercise or the like, it leads to an increase in the risk of falls and further lack of exercise, and as a result, locomotive syndrome progresses. Furthermore, in order to diagnose, prevent, and improve locomotive syndrome, the range of motion of joints is evaluated, and the exercise of moving joints by oneself or with the help of others is incorporated as rehabilitation to expand or increase the range of motion of joints. From this, it can be seen that the range of motion of joints is very important in locomotive syndrome. Therefore, it is very important to provide a more effective method for improving the range of motion, that is, a method for expanding / increasing the range of motion, in order to develop a method for preventing and improving locomotive syndrome (non-patented). Documents 1 and 2).

Collagen is a protein that makes up the locomotor organs such as ligaments, tendons, bones, and cartilage. There are 28 types of collagen depending on the amino acid sequence. Ligaments, tendons and bones are composed of type I collagen, and cartilage is composed of type II collagen. Type II collagen has been reported to have a composition for preventing and treating osteoarthritis (Patent Document 2) and an action of widening the range of motion of the knee (Non-Patent Document 3), and is also used in foods with functional claims. ing. However, its action to prevent or improve locomotive syndrome is not known.

Ampelopsis gland is a crude drug that has been taken for a long time in China, and its extract has been reported to be used as a preventive and ameliorating agent for rheumatoid arthritis due to its antioxidant effect (Patent Document 3). On the other hand, the action of Nogure to improve the range of motion of joints and the action to prevent and improve locomotive syndrome are not known.

N-Acetylglucosamine is a monosaccharide in which the 2-hydroxyl group of glucose is replaced with an acetylamino group. In the living body, it is abundantly contained in joint fluid and cartilage, and its use as an anti-arthritis agent has been reported (Patent Document 4). On the other hand, the action of N-acetylglucosamine to improve the range of motion of joints and the action to prevent or improve locomotive syndrome are not known.

The relationship between osteoarthritis, rheumatoid arthritis, arthritis and reduction / reduction of joint mobility is not known, and improvement of joint mobility is the most important for prevention and improvement of locomotive syndrome. Even if a method for simply preventing or improving osteoarthritis, rheumatoid arthritis, or arthritis (Patent Documents 3 to 4) is applied to locomotive syndrome, the joint range of motion cannot be improved, and for prevention and improvement of locomotive syndrome. Does not connect at all. Furthermore, there is no known method for improving the range of motion of joints by combining collagen, ampelopsis gland and N-acetylglucosamine, or for preventing or improving locomotive syndrome.

JP-A-2017-88524 JP-A-2009-51849 Patent No. 4563659 Patent No. 4754066

Kozo Nakamura, Journal of the Japan Geriatrics Society 49 (4), 393-401 (2012) Etsuo Chosa, Jpn. Rehavel. Med. 50, 48-54 (2013) Lugo et al. , J. Int. Soc. Sports. Nutr. 10:48 (2013)

An object of the present invention is a range of motion improving agent and a food composition for improving the range of motion containing collagen, novine and N-acetylglucosamine, and a locomotive syndrome preventing or improving agent and locomotive characterized by containing these. To provide a food composition for preventing or improving syndrome.

The collagen used in the present invention is obtained from fish skin, fish scales, pig skin and the like by a known method such as acid, alkali and heat treatment. A collagen peptide obtained by treating the obtained collagen with a proteolytic enzyme or an acid may be used. It is also possible to use commercially available collagen or collagen peptide, or collagen peptide obtained by treating commercially available collagen with proteolytic enzyme or acid. In the present invention, it is particularly preferable to utilize collagen peptide.

The amount of collagen used in the present invention can be appropriately adjusted according to the dosage form, purpose of use, age, body weight and the like. The daily usage amount is preferably 100 mg to 15000 mg, and particularly preferably 2000 mg to 6000 mg. In some cases, an amount smaller than the above range is sufficient, and in other cases, an amount exceeding the above range is required.

Ampelopsis glandulusa used in the present invention is a plant belonging to the genus Ampelopsis of the family Grapes, and is a vine deciduous shrub that grows naturally in the mountains of East Asia such as China and all over Japan from Hokkaido to Okinawa. be. Unlike the genus Ampelopsis, which is used as a raw material for raw food and wine, the genus Ampelopsis has a bad taste, so it is used as a Chinese medicine and horticultural plant. Also known as wild grapes, snake grapes, dog grapes, and crow grapes. In the present invention, a plant belonging to the genus Ampelopsis of the Grapes family can be used, and the site to be used is not particularly limited. Roots, or a mixture thereof, etc.), etc., and it is particularly preferable to use the above-ground part.

In the present invention, the whole or part of the plant body of Ampelopsis gland may be used as it is, or it may be dried, crushed, shredded or the like. Alternatively, an extract in a solvent may be used. For the extraction, the whole or part of the plant body of Ampelopsis gland may be used as it is, or may be extracted after being subjected to treatments such as drying, crushing, and shredding. Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.) and liquid polyhydric alcohols (1,3-butylene glycol, propylene). Glycol, glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.) Can be mentioned. These solvents may be used alone or in admixture of two or more. In the present invention, it is preferable to use the above-ground extract, and the extraction solvent is preferably a polar solvent such as water or lower alcohol, particularly hydrous ethanol.

The above extract may be used as it is in the extracted solution, or may be used after being concentrated, diluted, filtered, decolorized with activated carbon or the like, deodorized, ethanol precipitation or the like, if necessary. Further, the extracted solution may be subjected to treatments such as concentrated drying, spray drying, freeze drying and the like, and used as a dried product.

The amount of ampelopsis gland used in the present invention can be appropriately adjusted according to the dosage form, purpose of use, age, body weight and the like. The daily amount of the dried product of Ampelopsis gland extract is preferably 0.1 mg to 2000 mg, and particularly preferably 1 mg to 50 mg. In some cases, an amount smaller than the above range is sufficient, and in other cases, an amount exceeding the above range is required.

The N-acetylglucosamine used in the present invention is obtained from a natural polysaccharide chitin derived from the outer shell of crustaceans such as crabs and shrimp by a known method such as hydrolysis with an acid and an enzyme. It is also possible to use a commercially available product.

The amount of N-acetylglucosamine used in the present invention can be appropriately adjusted depending on the dosage form, purpose of use, age, body weight and the like. The daily usage amount is preferably 10 mg to 5000 mg, particularly preferably 200 mg to 2000 mg. In some cases, an amount smaller than the above range is sufficient, and in other cases, an amount exceeding the above range is required.

The range of motion of the joint in the present invention can be any part of the joint such as the knee, hip, shoulder, hip, jaw, cervical spine, and spine, and is particularly effective for the knee joint.

The locomotive syndrome in the present invention refers to a state in which daily life is restricted due to a disorder of the motor organs and the risk of requiring long-term care or becoming asleep is increased. Prevention and improvement of disuse muscular atrophy, fracture, osteoporosis, degenerative arthritis, rheumatoid arthritis, etc. are also included in the scope of the effect of the present invention. The site can be the whole body such as the arms, legs, and torso, and is particularly effective for symptoms that occur in the legs.

The range of motion improving agent and the locomotive syndrome preventing or improving agent of the present invention can be used as foods, quasi-drugs or pharmaceuticals. As foods, it can be used as beverages, hard capsules, soft capsules, tablets, granules, jellies and the like. For quasi-drugs and pharmaceuticals, oral powders, granules, tablets, sugar-coated tablets, capsules, syrups, pills, suspensions, liquids, emulsions, etc., parenteral injections and suppositories And so on.

The range of motion improving agent of the present invention is an excipient, a stabilizer, a preservative, a binder, a disintegrant used in ordinary foods, non-pharmaceutical products or pharmaceuticals, as needed, within a range that does not impair the effect. , Hydrocarbons, fatty acids, alcohols, esters, pH regulators, preservatives, fragrances and the like. Furthermore, it can also contain polysaccharides, plant extracts and the like. In the present invention, it is particularly preferable to contain hyaluronic acid, bear bamboo extract, linden extract, cat's claw extract and the like.

The food composition characterized by containing collagen, ampelopsis gland and N-acetylglucosamine of the present invention has a range of motion improving action, a locomotive syndrome preventing or improving action.

Examples will be given to explain the present invention in detail, but the present invention is not limited thereto. The% of the content shown in the examples represents% by weight.

An example of producing a solvent extract using Nogrape is shown below.

Production Example 1 30% Ethanol Extract of Ampelopsis Gland 350 mL of purified water and 150 mL of ethanol are added to 20 g of crushed above-ground part of Ampelopsis gland, extracted at room temperature for 5 days, and the filtrate is concentrated to dryness to dry the 30% ethanol extract of Ampelopsis gland. 9.9 g was obtained.

Production Example 2 50% Ethanol Extract of Ampelopsis Gland 250 mL of purified water and 250 mL of ethanol are added to 20 g of crushed above-ground part of Ampelopsis gland, extracted at room temperature for 5 days, and the filtrate is concentrated to dryness to dry and dry the 50% ethanol extract of Ampelopsis gland. 0.4 g was obtained.

Production Example 3 70% Ethanol Extract of Ampelopsis Gland 150 mL of purified water and 350 mL of ethanol were added to 20 g of the above-ground crushed product of Ampelopsis gland, extracted at room temperature for 6 days, and the filtrate was concentrated to dryness to extract 70% ethanol of Ampelopsis gland. .1 g was obtained.

Production Example 4 Nogure Ethanol Extract 1000 mL of ethanol was added to 40 g of the above-ground crushed product of Nogure, extracted at room temperature for 7 days, and then the filtrate was concentrated to dryness to obtain 1.2 g of Nogrape ethanol extract.

Production Example 5 Ampelopsis hot water extract Add 500 mL of purified water to 25 g of the above-ground crushed product of Ampelopsis gland, extract at 95-100 ° C. for 2 hours, concentrate the filtrate, and freeze-dry to extract 1 ampelopsis hot water. 8.8 g was obtained.

Prescription example 1 Beverage prescription content (%)
1. 1. Collagen peptide 13.30
2. No grape 30% ethanol extract (Production Example 1) 0.05
3. 3. N-Acetylglucosamine 2.00
4. Acesulfame potassium 0.01
5. Malic acid 1.00
6. Fragrance 0.50
7. Purified water 83.14
[Manufacturing method] Ingredients 1 to 6 are stirred and dissolved in a part of the component 7. The rest of ingredient 7 is then added, mixed, heated to 90 ° C. and filled in a 30 mL glass bottle.
[Usage] Take 1 bottle (30 mL) per day.

Prescription example 2 Beverage prescription content (%)
1. 1. Collagen 8.30
2. No grape 50% ethanol extract (Production Example 2) 0.02
3. 3. N-Acetylglucosamine 1.50
4. Sucralose 0.02
5. Citric acid 1.00
6. Fragrance 0.50
7. Purified water 88.66
[Manufacturing method] Ingredients 1 to 6 are stirred and dissolved in a part of the component 7. The rest of ingredient 7 is then added, mixed, heated to 90 ° C. and filled in a 30 mL glass bottle.
[Usage] Take 1 bottle (30 mL) per day.

Prescription example 3 Powder prescription content (%)
1. 1. Collagen 33.3
2. No grape 70% ethanol extract (Production Example 3) 0.1
3. 3. N-Acetylglucosamine 25.0
4. Dried cornstarch 20.0
5. Microcrystalline Cellulose 21.6
[Manufacturing method] Ingredients 1 to 5 are mixed to prepare a powder.
[Usage] Take 1 packet (3g) per day.

Prescription example 4 Tablet prescription Content (%)
1. 1. Collagen 15.0
2. No grape 30% ethanol extract (Production Example 1) 3.0
3. 3. N-Acetylglucosamine 40.0
4. Lactose 10.0
5. Cornstarch 5.0
6. Crystalline cellulose 24.5
7. Glycerin fatty acid ester 2.5
[Manufacturing method] Ingredients 1 to 6 are mixed, then 10% water is added as a binder, and the mixture is extruded and granulated, and then dried. Ingredient 7 is added to the molded granules, mixed, and locked. One tablet weighs 0.5 g.
[Usage] Take 4 tablets (2g) per day.

Prescription example 5 Powdered beverage prescription Content (%)
1. 1. Collagen peptide 77.8
2. No grape ethanol extract (Production Example 4) 0.3
3. 3. N-Acetylglucosamine 1.0
4. Dextrin 10.0
5. Pullulan 5.0
6. Sucrose fatty acid ester 5.9
[Manufacturing method] Ingredients 1 to 6 are mixed. Dissolves in water when ingested.
[Usage] Take 9g per day.

Prescription example 6 Granule prescription Content (%)
1. 1. Collagen 10.00
2. Ampelopsis hot water extract (Production Example 5) 0.05
3. 3. N-Acetylglucosamine 20.00
4. Lactose 49.95
5. Cellulose 20.00
[Manufacturing method] Ingredients 1 to 5 are granulated in a fluidized tank to obtain granules.
[Usage] Take 1 packet (1g) per day.

Comparative Example 1 Conventional Beverage In the beverage of Formulation Example 1, collagen peptide, 30% ethanol extract of ampelopsis gland, and N-acetylglucosamine were replaced with water to prepare a conventional beverage.

Comparative Example 2 Conventional Beverage Containing Collagen In the beverage of Formulation Example 1, a beverage obtained by substituting 30% ethanol extract of Ampelopsis gland and N-acetylglucosamine with water was used as a conventional beverage containing collagen.

Comparative Example 3 Conventional Beverage Containing Ampelopsis Gland In the beverage of Formulation Example 1, collagen peptide and N-acetylglucosamine were replaced with water to prepare a conventional beverage containing ampelopsis gland.

Comparative Example 4 Conventional Beverage Containing N-Acetylglucosamine In the beverage of Formulation Example 1, the collagen peptide and the 30% ethanol extract of Ampelopsis gland were replaced with water to prepare a conventional beverage containing N-acetylglucosamine.

Comparative Example 5 Conventional Beverage Containing Collagen and Ampelopsis Gland In the beverage of Formulation Example 1, a beverage in which N-acetylglucosamine was replaced with water was used as a conventional beverage containing collagen and ampelopsis gland.

Comparative Example 6 Conventional Beverage Containing Collagen and N-Acetylglucosamine In the beverage of Formulation Example 1, a beverage in which the 30% ethanol extract of Ampelopsis gland was replaced with water was used as a conventional beverage containing collagen and N-acetylglucosamine. ..

Comparative Example 7 Conventional Beverage Containing Ampelopsis Gland and N-Acetylglucosamine In the beverage of Formulation Example 1, a beverage in which collagen peptide was replaced with water was used as a conventional beverage containing Ampelopsis gland and N-acetylglucosamine.

Test Example 1 Improvement of joint range of motion by combined use of collagen, no grape and N-acetylglucosamine 80 men and women (40-79 years old) who feel difficulty in moving their knee joints in daily life are divided into 8 groups of 10 each. , Beverages containing collagen, no grapes and N-acetylglucosamine (Prescription Example 1), conventional beverages (Comparative Example 1), collagen-containing beverages (Comparative Example 2), and beverages containing no grapes (Comparative Example 3). ), N-acetylglucosamine-containing beverage (Comparative Example 4), collagen and no grape-containing beverage (Comparative Example 5), collagen and N-acetylglucosamine-containing beverage (Comparative Example 6), no grape and N-acetyl. Using a beverage containing glucosamine (Comparative Example 7), 30 mL was ingested once a day for 3 months. As an evaluation of the range of motion of the joint, take a picture of the knee bent in the prone position and the recumbent position, and refer to the "range of motion display and measurement method" of the Japanese Society of Orthopedic Surgery and the Japanese Society of Rehabilitation Medicine, from the thigh. The knee flexion angle was calculated by measuring the angle of intersection between the straight line connecting the knees and the straight line connecting the ankles to the knees.

Table 1 shows the results of the knee joint flexion angle in the prone position of Test Example 1, and Table 2 shows the results of the knee joint flexion angle in the supine position. As a result, as compared with the group ingesting the conventional beverage, the group ingesting collagen and no grapes alone, the group ingesting the beverage containing collagen and no grapes, and the beverage containing collagen and N-acetylglucosamine were ingested. In the group that received the beverage and the group that ingested the beverage containing N-acetylglucosamine, improvement in the knee joint flexion angle was observed in the prone and supine positions. Furthermore, the group ingesting the beverage containing collagen, ampelopsis gland and N-acetylglucosamine significantly improved the knee flexion angle in the prone and supine positions as compared with the other groups. Therefore, it was clarified that the combined use of collagen, ampelopsis gland and N-acetylglucosamine showed an extremely remarkable effect of improving the range of motion of the joint, and further had an extremely remarkable effect of preventing or improving locomotive syndrome.

Test Example 2 The combined use of collagen, no grape and N-acetylglucosamine improves knee discomfort and pain. 80 men and women (40-79 years old) who feel discomfort and pain in their daily lives are divided into 8 groups of 10 each. Beverages containing collagen, no grapes and N-acetylglucosamine (Prescription Example 1), conventional beverages (Comparative Example 1), collagen-containing beverages (Comparative Example 2), and beverages containing no grapes (Comparative Examples). 3), Beverage containing N-acetylglucosamine (Comparative Example 4), Beverage containing collagen and no grape (Comparative Example 5), Beverage containing collagen and N-acetylglucosamine (Comparative Example 6), No grape and N- Using a beverage containing acetylglucosamine (Comparative Example 7), 30 mL was ingested once a day for 3 months. The discomfort and pain of the knee during daily activities (resting, walking on level ground, climbing stairs, bending and stretching legs, getting up, and standing) were evaluated by the Visual Analog Scale (VAS).

Table 3 shows the results of VAS of discomfort and pain in the knee when a beverage containing collagen, ampelopsis gland and N-acetylglucosamine of Test Example 2 was ingested. In the group that ingested a beverage containing collagen, no grape and N-acetylglucosamine, when walking on level ground, climbing stairs, bending and stretching legs, getting up, standing up, knee discomfort, resting, walking on level ground, Knee pain improved when going up and down stairs, bending and stretching legs, getting up, and standing. This was a very significant effect as compared with the case of ingesting the beverages of other comparative examples. From this, it can be said that the combined use of collagen, ampelopsis gland and N-acetylglucosamine shows an extremely remarkable preventive or ameliorating effect on locomotive syndrome.

It was clarified that the combined use of collagen, ampelopsis gland and N-acetylglucosamine showed an extremely remarkable effect of improving the range of motion of joints. Since it has the above-mentioned actions, a locomotive syndrome preventive or ameliorating agent or a locomotive syndrome preventive or ameliorating food composition can be obtained by using collagen, ampelopsis gland and N-acetylglucosamine in combination.

Since the present invention has a range of motion improving action, it can be used for foods, quasi-drugs or pharmaceuticals for the purpose of preventing or improving locomotive syndrome. In addition, the present invention makes it possible to obtain a significantly higher preventive or ameliorating effect as compared with the conventional invention.

Claims (4)

A range of motion improving agent characterized by containing collagen, ampelopsis gland and N-acetylglucosamine. A food composition for improving range of motion, which comprises collagen, ampelopsis gland and N-acetylglucosamine. A locomotive syndrome preventive or ameliorating agent, which comprises the range of motion improving agent according to claim 1. A food composition for preventing or improving locomotive syndrome, which comprises the food composition for improving the range of motion according to claim 2.