JP6575984B2 - Solution stirring device - Google Patents

Solution stirring device Download PDF

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Publication number
JP6575984B2
JP6575984B2 JP2015256825A JP2015256825A JP6575984B2 JP 6575984 B2 JP6575984 B2 JP 6575984B2 JP 2015256825 A JP2015256825 A JP 2015256825A JP 2015256825 A JP2015256825 A JP 2015256825A JP 6575984 B2 JP6575984 B2 JP 6575984B2
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vibration
liquid
voice coil
coil motor
stirred
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JP2017119243A (en
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田代 英夫
英夫 田代
野田 紘憙
紘憙 野田
武利 鳥山
武利 鳥山
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D−テック合同会社
ケーディークロート株式会社
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Description

  The present invention relates to a solution stirring apparatus that stirs a liquid to be stirred such as a reagent or a specimen liquid.

  Conventionally, a vortex mixer and a magnetic stirrer are known as devices for stirring reagents in a laboratory where experiments and analyzes are performed. The vortex mixer has a rotating part that rotates. For example, when the bottom of the test tube containing the liquid to be stirred is pressed against the rotating part, the test tube is shaken and shaken. The stirring solution is stirred. In addition, in the magnetic stirrer, for example, a stirrer is stored together with a liquid to be stirred in a beaker, and the stirred liquid is stirred by rotating the stirrer remotely by magnetic force from the bottom of the beaker. . That is, in the conventional vortex mixer and the magnetic stirrer, the liquid to be stirred is stirred using the rotational force of the rotating member.

On the other hand, the structure which stirs a to-be-stirred liquid without using the rotating member is also known, and the technique of the following patent documents 1 and 2 is known.
In Japanese Patent Application Laid-Open No. 2001-327846 as Patent Document 1, a vibrating part (2) composed of a piezoelectric element that vibrates in the vertical direction is supported on an upper part of a pedestal (3), and further, a vibrating part (2) The plate-like support part (1) is supported with respect to the upper part, and the configuration in which the droplet (A) is supported on the surface (10) on the support part (1) is described. In Patent Document 1, the vibration frequency of the piezo element is swept in one direction to vibrate the liquid droplet (A), causing the surface tension wave to resonate in the liquid droplet (A), and the liquid droplet (A). Is described. In Patent Document 1, the droplet (A) has a contact angle of 70 ° or more with respect to the surface (10), and the droplet (A) has a configuration of 5 μL.

  In Japanese Patent No. 3879676 as Patent Document 2, a microtiter plate (7) having a plurality of wells (7a) is supported by a mounting table (6), and vibration of a lower vibration actuator (22) is The structure transmitted to a mounting table (6) via a spring member (23) is described. In Patent Document 2, the vibration frequency of the vibration actuator (22) is gradually increased to cause resonance in the microtiter plate (7) and the like, and the dissolution of the solvent and the compound in the microtiter plate (7) is promoted. . In Patent Document 2, the output end (22a) of the vibration actuator (22) extends in the horizontal direction, and a leaf spring member (23) extending in the vertical direction with respect to the output end (22a) is supported. The upper end of the member (23) is supported by the mounting table (6). Therefore, in Patent Document 2, when the vibration actuator vibrates, the output end (22a), the leaf spring member (23), and the mounting table (6) are configured to vibrate in the horizontal direction, and the microtiter plate (7 ) Vibrates transversely to the direction in which vibration is transmitted.

JP 2001-327846 A ("0012", "0018", "0019", FIG. 1) Japanese Patent No. 3879676 ("0021" to "0036", FIG. 2)

In a magnetic stirrer, a stirrer is placed in a liquid to be stirred and rotated. Therefore, when the container is shallow, the liquid is likely to scatter when the stirring bar rotates. In addition, when the container is shallow, the stirring bar may not be placed in the container in the first place. Therefore, the magnetic stirrer is not suitable for stirring the liquid in a shallow container.
Further, in the vortex mixer, the rotating part rotates around an axis extending in the direction of gravity, and the container in contact with the rotating part is shaken in the horizontal direction. At this time, in the container, the liquid to be stirred is likely to be biased toward the outer peripheral side due to centrifugal force or hit the side wall. Therefore, when a shallow container is used, there is a possibility that the liquid to be stirred is scattered in the vortex mixer.

Furthermore, Patent Document 2 describes a configuration in which the liquid is stirred by vibrating the microtiter plate (7) by the vibration of the vibration actuator (22). However, the microtiter plate (7) of Patent Document 2 vibrates laterally and vibrates in the horizontal direction. Therefore, the liquid is also easily shaken in the horizontal direction, and the liquid may be scattered if the microtiter plate (7) is not deep.
Therefore, the conventional stirring device using the rotational force and the configuration described in Patent Document 2 are not suitable for stirring the liquid contained in the shallow container.

  On the other hand, Patent Document 1 describes a configuration in which a piezo element is vibrated in a gravitational direction to stir droplets on a plate member. However, in the piezo element, generally generated amplitude is small. Here, according to experiments by the inventors of the present application, when the amplitude of vibration is too small, it has been confirmed that the liquid to be stirred is difficult to flow. In the first place, in Patent Document 1, a droplet having a contact angle of 70 ° or more is targeted, and a 5 μL droplet is stirred. Therefore, it is difficult to simply apply the configuration of Patent Document 1 to a configuration in which, for example, 100 μL of liquid is stirred.

  This invention makes it a technical subject to stir the to-be-stirred liquid of the quantity of 200 microliters or less in the accommodating part which has a depth of 1.5 mm or less.

In order to solve the technical problem, the solution agitating device according to claim 1 comprises:
A to-be-vibrated portion having a containing portion in which an amount of liquid to be stirred of 200 μL or less is contained and has a depth of 1.5 mm or less;
A voice coil motor that vibrates along the vertical direction and imparts vibration to stir the liquid to be stirred, with the force that the coil through which the current flows receives by the magnetic field as an operating principle;
A vibration transmitting unit that is supported by the voice coil motor and configured to be capable of point contact with the vibrating unit, and transmits the vibration of the voice coil motor to the housing unit;
Control means for oscillating the voice coil motor, the control means for oscillating the voice coil motor while changing the frequency in a predetermined frequency range around the resonance frequency of the oscillated portion;
It is provided with.

Invention of Claim 2 is the solution stirring apparatus of Claim 1,
The control means for vibrating the voice coil motor while changing in the frequency range based on the primary or secondary resonance frequency of the vibration part;
It is provided with.

Invention of Claim 3 is the solution stirring apparatus of Claim 1 or 2,
When stirring the liquid to be stirred, a vibration with a first amplitude set in advance is applied, and when the voice coil motor is started, the first amplitude is reduced to a second amplitude smaller than the first amplitude. The control means for raising the first rise time from the second amplitude to the first amplitude at a second rise time shorter than the first rise time after being raised at the rise time of one;
It is provided with.

According to the first aspect of the present invention, compared to the case where the configuration of the present invention is not provided, the liquid to be stirred of 200 μL or less is agitated in a short time in a container having a depth of 1.5 mm or less. Can do.
According to the second aspect of the present invention, it is possible to easily reduce the frequency for vibrating the voice coil motor and to make the liquid to be stirred follow the vibration as compared with the case of third-order or higher resonance.
According to the third aspect of the present invention, it is possible to suppress scattering of the liquid to be agitated as compared with the case where the first amplitude is raised at a stroke.

FIG. 1 is an explanatory view of a solution stirring apparatus according to a first embodiment of the present invention, FIG. 1A is an overall explanatory view, and FIG. 1B corresponds to a case when viewed from above, and a vibration transmitting portion is in contact with a vibrating portion having a chip cassette. It is explanatory drawing of the position to perform. FIG. 2 is an explanatory diagram of the frequency at which the voice coil motor of the solution stirring apparatus is vibrated and the resonance frequency at which the vibration mode is generated in the solution stirring apparatus in the solution stirring apparatus according to the first embodiment of the present invention. FIG. 3 is an explanatory diagram of Experimental Example 1, and is an explanatory diagram of measurement results of frequency and amplitude. FIG. 4 is an explanatory diagram of Experimental Example 2 and Experimental Example 3. FIG. 4A is an explanatory diagram of the arrangement position of the chip cassette of Experimental Example 2. FIG. 4B is an explanatory diagram when the chip cassette is vibrated by point contact. 4C is an explanatory diagram for comparison when the chip cassette is vibrated by surface contact, and FIG. 4D is an explanatory diagram for the arrangement position of the chip cassette of Experimental Example 3. FIG.

Next, specific examples of embodiments of the present invention (hereinafter referred to as examples) will be described with reference to the drawings. However, the present invention is not limited to the following examples.
In order to facilitate understanding of the following description, in the drawings, the front-rear direction is the X-axis direction, the left-right direction is the Y-axis direction, the up-down direction is the Z-axis direction, and arrows X, -X, Y, -Y, The direction indicated by Z and -Z or the indicated side is defined as the front side, the rear side, the right side, the left side, the upper side, the lower side, or the front side, the rear side, the right side, the left side, the upper side, and the lower side, respectively.
In the figure, “•” in “○” means an arrow heading from the back of the page to the front, and “×” in “○” is the front of the page. It means an arrow pointing from the back to the back.
In the following description using the drawings, illustrations other than members necessary for the description are omitted as appropriate for easy understanding.

FIG. 1 is an explanatory view of a solution stirring apparatus according to a first embodiment of the present invention, FIG. 1A is an overall explanatory view, and FIG. 1B corresponds to a case when viewed from above, and a vibration transmitting portion is in contact with a vibrating portion having a chip cassette. It is explanatory drawing of the position to perform.
In FIG. 1, the solution stirring apparatus 1 of Example 1 has the voice coil motor 2 which can vibrate along the up-down direction on the principle of operation which the coil which an electric current flows receives with a magnetic field. The voice coil motor 2 according to the first embodiment is formed in a cylindrical shape extending vertically, and a vibration source body is disposed at a circular center portion. In the voice coil motor 2 according to the first embodiment, the vibration source body is supported so as to be movable in the vertical direction. A coil is wound around the vibration source body, and the coil is configured to be energized. The vibration source body is disposed in a magnetic field having a direction along the horizontal direction. Here, according to Fleming's left-hand rule, the direction of the force that the coil portion through which the current flows is subjected to the magnetic field is orthogonal to the direction in which the current flows and is orthogonal to the direction of the magnetic field. Therefore, in the voice coil motor 2 according to the first embodiment, when a current flows through the coil, a force along the vertical direction acts on the coil, and the vibration source body vibrates in the vertical direction.
An extension cylinder 3 extending upward is supported at the upper center of the voice coil motor 2 as an example of a vibration transmitting portion. The extension cylinder 3 has a lower cylindrical part 3a supported by the voice coil motor 2, and a contact part 3b formed above the cylindrical part 3a and having a diameter that decreases toward the upper part.

Above the extension cylinder 3, a portion to be vibrated 11 to which vibration is imparted by the voice coil motor 2 is disposed. The portion to be vibrated 11 includes a chip cassette 12 in which the liquid to be stirred L is accommodated, and a holder 13 on which the chip cassette 12 is detachably supported.
The chip cassette 12 as an example of the container has a cassette body 14 in which the liquid to be stirred L is accommodated. A bottom portion 15 having a regular square plate shape is formed at the lower portion of the cassette body 14. In addition, in Example 1, although the bottom part 15 illustrated the structure of regular square plate shape, it is not limited to this. For example, the bottom portion 15 can be formed into a plate shape of an arbitrary shape such as a circular plate shape or a rectangular plate shape. On the upper surface of the bottom portion 15, a stirring portion 16 as an example of a storage portion is formed corresponding to the position of the center of gravity. The stirring unit 16 of the first embodiment is formed in a circular shape that is recessed with respect to the upper surface of the bottom portion 15. As for the stirring part 16, the depth h from the upper surface of the bottom part 15 is set to 1.5 [mm] or less. The bottom surface of the stirring unit 16 is treated to be hydrophilic.

The stirring unit 16 is configured to be able to contain a liquid to be stirred L of 200 [μL] or less. In particular, 50 [μL] to 150 [μL] of the liquid to be stirred L can be suitably stored in the stirring unit 16. The stirring unit 16 can store any liquid to be stirred, such as a reagent and a target liquid of the reagent, a specimen liquid, a cleaning liquid, an antibody reagent, and a luminescent reagent, as the liquid to be stirred.
In addition, in Example 1, although the stirring part 16 illustrated the recessed structure, it is not limited to this. For example, it is possible to form a hydrophobic part in an annular shape on the upper surface of the bottom part 15 and to use the inside of the hydrophobic part as a stirring part. That is, the depth h can be set to 0 [mm], and the stirring unit 16 can set the depth h to 0 [mm] ≦ h ≦ 1.5 [mm]. In addition, about a hydrophobic part, since the structure as described in Unexamined-Japanese-Patent No. 2013-24605 etc. is applicable, detailed description is abbreviate | omitted. Note that 200 [μL] is the volume of the stirring unit. Further, depending on the liquid properties such as the surface tension and viscosity of the liquid L to be stirred, the liquid may be easily overflowed at 150 [μL] or more, and the amount may be too small to be stirred at 50 [μL] or less.

  A container wall 17 extending upward is supported on the top, bottom, left, and right of the bottom portion 15. The bottom 15, the agitation unit 16, and the container wall 17 constitute the cassette body 14 of the first embodiment. In the first embodiment, a cover 18 that covers the upper portion of the cassette body 14 is supported on the upper end of the container wall 17. In the cover 18, a supply port 18 a for the liquid to be stirred L is formed so as to correspond to the upper part of the stirring unit 16. The cassette main body 14 and the cover 18 constitute the chip cassette 12 of the first embodiment. In addition, the bottom part 15 and the cover 18 of the chip cassette 12 of Example 1 are comprised with the transparent member, and are comprised so that the state of the to-be-stirred liquid L can be observed from the exterior. Here, in the first embodiment, the configuration in which both the bottom portion 15 and the cover 18 are transparent members is illustrated, but the present invention is not limited to this. For example, it is possible to configure only one side with a transparent member, for example, the bottom 15 is configured with a transparent member and the cover 18 is configured with a non-transparent member. Further, when not observing from the outside, both the bottom portion 15 and the cover 18 can be made of a non-transparent member.

  In FIG. 1, a holder 13 as an example of a container holding unit includes a plate-shaped temperature control unit 21 that supports the lower surface of the chip cassette 12, and a side wall unit 22 that is arranged corresponding to the outer peripheral shape of the chip cassette 12. Have Therefore, when the chip cassette 12 is mounted on the holder 13, the chip cassette 12 is held in contact with the temperature control unit 21 and the side wall unit 22. The temperature control unit 21 can be configured, for example, by a sandwich structure in which a high-resistance conductive polymer is sandwiched between polyimide films, and is configured to generate heat when power is supplied from the outside. That is, the temperature control unit 21 is configured to maintain the chip cassette 12 at a preset temperature, for example, 38 degrees, and to promote the reaction of the liquid L to be stirred inside.

  In addition, as a temperature control part, it is not limited to the illustrated temperature control part 21, Arbitrary temperature control structures can be used. For example, when the heating element is attached to the bottom surface of the aluminum block to form the temperature control unit 21 and the chip cassette 12 is mounted on the holder 13, the bottom surface of the chip cassette 12 comes into contact with the heating aluminum block, thereby the chip cassette. The structure which heats the to-be-stirred liquid L in 12 is also possible. In addition, it is also possible to utilize the effect of the residual heat according to the heat capacity of the aluminum block for assisting in heating.

Moreover, the temperature control part 21 is not limited to the structure fixedly supported by the holder 13, The structure which makes the temperature control part 21 approaching / separating with respect to the holder 13 is also possible. For example, the temperature control unit 21 is formed of a heat-generating aluminum block, and an opening is provided in the lower part of the holder 13 instead of the temperature control unit 21. The heating aluminum block is brought close to the holder 13 during heating and brought into contact with the bottom surface of the chip cassette 12 through the opening, and when not heated, the heating aluminum block is separated from the bottom surface of the chip cassette 12. Can also be conceived. Therefore, it is conceivable that the chip cassette 12 can be observed from below while being supported by the holder 13 by separating an aluminum block or the like.
The chip cassette 12 and the holder 13 constitute the vibrating portion 11 of the first embodiment.

  Here, in Example 1, the vibration part 11 and the extension cylinder 3 are held in contact with each other. At this time, the contact portion 3b has a convex shape upward, and the lower surface of the temperature control portion 21 has a planar shape. Therefore, the extension cylinder 3 makes point contact with the temperature control unit 21. In particular, in the first embodiment, as shown in FIG. 1B, the point contact position P <b> 1 is set corresponding to the center position of the stirring unit 16 of the chip cassette 12. Therefore, the vibration is transmitted from the position P1 to the vibrating part 11 of the first embodiment.

The solution stirring apparatus 1 has a control unit C that controls the voice coil motor 2.
The control unit C includes an input / output interface I / O for inputting / outputting signals from / to the outside, a ROM storing a program and information for performing necessary processing, a read-only memory, and temporarily storing necessary data. RAM for storing: a random access memory, a CPU for performing processing in accordance with a program stored in a ROM, etc .: a central processing unit and the like. Therefore, the control part C of Example 1 is comprised by information processing apparatus, what is called a computer. Therefore, the control part C can implement | achieve various functions by running the program memorize | stored in ROM etc.

The control unit C according to the first embodiment includes a stirring unit C1 as an example of a control unit that vibrates the voice coil motor 2.
The stirring means C1 includes an amplitude control means C1a and a frequency control means C1b. The agitation unit C1 vibrates the voice coil motor 2 based on the resonance frequency of the portion to be vibrated 11 and fluctuating in a preset frequency range A. The stirring means C1 of the first embodiment vibrates the voice coil motor 2 via a drive circuit (not shown) including a function generator and an amplifier that output a sine wave.
The amplitude control means C1a controls the amplitude of vibration of the voice coil motor 2. In the amplitude control means C1a of the first embodiment, the amplitude is increased from the start of stirring (0 [Vpp]) to 10 [Vpp] with a rise time Tr = 10 [seconds], and 10 [Vpp] to 20 [Vpp]. Until the rise time Tr = 2 [seconds]. During the subsequent stirring, the amplitude is maintained at 20 [Vpp].

FIG. 2 is an explanatory diagram of the frequency at which the voice coil motor of the solution stirring apparatus is vibrated and the resonance frequency at which the vibration mode is generated in the solution stirring apparatus in the solution stirring apparatus according to the first embodiment of the present invention.
Referring to FIG. 2, theoretically, the resonance frequency that causes the vibration system to generate a vibration mode decreases as the mass of the vibration system increases.
That is, in the vibration system that vibrates by the voice coil motor 2, when the chip cassette 12 is not set, it is assumed that the resonance frequency of the primary mode is f1 and the resonance frequency of the secondary mode is f2. At this time, when the chip cassette 12 is set in the voice coil motor 2 and the mass of the vibration system is increased, the resonance frequencies f1 and f2 of each vibration mode are shifted in principle in the direction of decreasing the frequency. That is, in FIG. 2, in principle, the resonance frequencies f1 and f2 corresponding to the solid line before the chip cassette 12 is set are shifted to the resonance frequencies f1 ′ and f2 ′ indicated by the one-dot chain line after the chip cassette 12 is set. To do.

  Here, the frequency control means C1b controls the frequency of vibration of the voice coil motor 2. That is, the frequency control means C1b varies the frequency of vibration in the frequency range A. The frequency range A is set based on the resonance frequency of the vibration part 11. The frequency range A of the first embodiment is set based on the low-order resonance frequency of the vibration part 11.

  Specifically, in the first embodiment, the frequency range A is set to include a resonance frequency f2 ′ that causes the vibration part 11 to generate a secondary vibration mode, that is, a so-called secondary mode. The frequency range A is set by experiment. In the first embodiment, as the frequency range A, a frequency range that is spread 5 [Hz] up and down around the resonance frequency 52 [Hz] of the secondary mode is set. The frequency control means C1b modulates the frequency within a range of ± 5 [Hz] at intervals of an upper limit of 1 second and a lower limit of 2 seconds with 52 [Hz] as the center. That is, the frequency control unit C1b according to the first embodiment modulates the frequency with a period of 1 second to 2 seconds when modulating the frequency within a range of 47 to 57 [Hz].

  The specific numerical values such as the center frequency 52 [Hz] and the modulation range ± 5 [Hz] set in the first embodiment are not limited to the exemplified values. Therefore, if the chip cassette has a different configuration such as shape and material, the center frequency and the modulation range can be changed. In other words, the frequency control unit C1b according to the first embodiment is configured to be easily changeable by executing software (not shown) so that the set value to be used can be corrected to the optimum value according to the weight and shape of the chip cassette. Yes.

(Operation of Example 1)
In the solution agitation apparatus 1 of Example 1 having the above-described configuration, the chip cassette 12 is mounted on the holder 13 in a state where the agitated liquid L of 200 [μL] or less as an example of the solution is accommodated in the agitation unit 16. Is done. Here, the depth h of the stirring unit 16 of the chip cassette 12 is set to 1.5 [mm] or less. In addition, since the bottom surface of the stirring unit 16 that is in contact with the liquid to be stirred L is treated to be hydrophilic, the thickness of the liquid to be stirred L tends to be about 1.5 [mm] or less. It is supported by the stirring unit 16 in a layered liquid form. Therefore, a thin layered liquid L to be stirred is set in the solution stirring apparatus 1 of the first embodiment. And in the solution stirring apparatus 1, if there exists an input of stirring start, the stirring means C1 will control the voice coil motor 2, and will vibrate the voice coil motor 2 to an up-down direction.

  At this time, vibration along the vertical direction is transmitted to the upper vibration-receiving portion 11 via the extension cylinder 3. Therefore, the vibration part 11 vibrates based on the vertical vibration. At this time, the frequency of the voice coil motor 2 is periodically modulated in the preset frequency range A. Here, the chip cassette 12 of Example 1 has a substantially plate-like flat configuration. Therefore, when the chip cassette 12 vibrates, vibration including not only bending vibration but also twisting vibration is likely to occur. Therefore, the thin layered liquid to be stirred L in the chip cassette 12 is stirred by the action of the vibration mode that vibrates in the twisting direction.

  Here, in the solution stirring apparatus 1 of Example 1, the frequency is modulated. Therefore, the vibration part 11 vibrates while the vibration mode is generated or eliminated. According to the experiments by the present inventors, it was confirmed that when the voice coil motor 2 is controlled only by the resonance frequency, the stirring of the liquid to be stirred L is difficult to proceed. This is probably because in the vibration mode generated by the resonance frequency, the liquid vibrates greatly at the so-called antinode position, but the liquid hardly vibrates at the node position. Therefore, in the control fixed at the resonance frequency, the positions of the antinodes and nodes are fixed, so-called standing waves (standing waves) are generated, and the range in which the liquid flows is likely to be limited. On the other hand, in Example 1 where the frequency is modulated, it is considered that the position of the antinodes and nodes of the standing wave changes, and the liquid L to be stirred is more likely to be mixed as compared with the case where the frequency is not modulated.

  For example, in a cantilever beam, it is known that a bending-only vibration mode without twisting occurs, but in a bending-only vibration mode, compared to a vibration mode with twisting, the resonance mode The vibration waveform tends to be simple. That is, even if it is going to stir the liquid L to be stirred in the bending vibration mode, the amplitude direction tends to be biased in one direction, such as the vertical direction, and there is a possibility that it takes time for stirring. On the other hand, in the vibration mode that vibrates also in the twisting direction, the vibration in the twisting direction is applied to the vertical direction, and the force in the direction of stirring the liquid L to be stirred easily acts. Therefore, in Example 1 including the vibration mode that vibrates in the twisting direction with respect to the vibration part 11, the agitation is easier than in the bending-only vibration mode.

  Moreover, the vibration mode of Example 1 is a low-frequency secondary mode. Here, in general, the higher the vibration mode, the higher the frequency and the faster the change in vibration. Further, when the order becomes higher, in the vibration part 11, the belly, the node, etc. increase, the rigidity of the vibration part 11 easily acts as a resistance, and the amplitude becomes small. Therefore, the liquid to be stirred L is difficult to follow sufficiently, and there is not enough energy to stir the liquid L to be stirred, so that the liquid or stirring of the liquid to be stirred L may be insufficient. Therefore, the lower order vibration mode is considered preferable for stirring. That is, a lower order vibration mode such as a second order is desirable for stirring compared to a higher order vibration mode, and a lower order is more preferable.

  In addition, according to an experiment to be described later, it was confirmed that stirring is difficult to proceed at the resonance frequency f1 ′ of the primary mode of Example 1. Here, in the experiment, the resonance frequencies f1 and f1 ′ of the primary mode before and after the chip cassette 12 are attached are hardly shifted before and after the chip cassette 12 is attached. Therefore, the resonance frequency f1 ′ of the primary mode is a resonance frequency corresponding to the resonance frequency f1 of only the vibration module and vibrates greatly, but since it is not based on the resonance frequency of the vibration part 11, stirring is difficult to proceed. It is thought that.

  Moreover, in Example 1, the vibration part 1 is vibrated by transmitting the vibration of an up-down direction. Here, in a conventional stirring device such as a vortex mixer, the liquid is stirred by a horizontal vibration along a horizontal direction such as a rotational force. However, a thin-layered liquid is easily affected by viscosity and surface tension, and hardly vibrates due to vibration along the bottom surface. Therefore, it is necessary to increase the vibration force in order to flow the thin layered liquid to be stirred L, and in the conventional configuration, the liquid to be stirred L is continuously biased to one side by centrifugal force and is not stirred. There was a problem that the liquid splashed on the wall. On the other hand, in Example 1 in which vibration along the vertical direction is transmitted, the liquid is less likely to come out from the stirring unit 16 to the side as compared with the case where vibration along the bottom surface acts.

Moreover, in Example 1, when the start of stirring is input, the amplitude is not increased to 20 Vpp, which is the target value from the beginning, in a short time (Tr = 2 seconds), but relatively up to 10 [Vpp]. Raise slowly (Tr = 10 seconds). As a result of the experiment, when the target value was increased to a stirrable value in a short time, the reagent or the like might scatter, but when it was performed relatively slowly, the reagent could be prevented from splattering.
Therefore, in Example 1, it is easy to stir in a state where scattering is suppressed.

  In the first embodiment, the extension cylinder 3 is in point contact with the temperature adjustment portion 21 of the holder 13. Here, when the extension cylinder 3 is omitted, the configuration is such that the upper surface of the voice coil motor 2 is in surface contact with the temperature control unit 21. The surface contact is considered to contact at a single point on the surface when viewed microscopically, and the contact position between the voice coil motor 2 and the portion to be vibrated 11 is likely to vary due to surface tolerances and variations in inclination during vibration. Therefore, the vibration of the portion to be vibrated 11 is not stable, and the stirring may become unstable. On the other hand, in the first embodiment, vibration is transmitted by point contact through the extension cylinder 3. Therefore, vibration is easily transmitted to the portion to be vibrated 11 at a fixed position. Therefore, in the first embodiment, it is easy to cause the vibration part 11 to stably generate the vibration mode.

In the first embodiment, the voice coil motor 2 is controlled at a so-called audible frequency range. Here, although an experiment was performed using ultrasonic vibrations, that is, a high-frequency vibration source on the order of kHz or MHz, stirring was not performed. Although the cause is unknown, it is thought that the liquid is difficult to flow because the wavelength is too short or the amplitude is too small.
In addition, when vibrations having a frequency in the audible sound range are transmitted in a non-contact manner, that is, via a gas or liquid, using a speaker, the vibrations cannot be sufficiently transmitted to the vibration target part, and stirring cannot be performed.

(Experimental example 1)
Next, an experiment for confirming the effect of the example was performed.
In Experimental Example 1, a vibration module having a diameter of 115 [mm] and a rated maximum input of 20 [W] was used as the voice coil motor 2. The vibration module is a vibration mechanism that is basically the same in structure and structure as the speaker, and is a vibration mechanism in which a vibration plate is fixed to a voice coil motor arranged at the center of the vibration module. Mechanism. That is, in the speaker, the cone paper is fixed to the voice coil motor arranged in the center, but in the vibration module, the vibration plate is fixed instead of the cone paper. In Experimental Example 1, the relationship between the frequency [Hz] and the amplitude [mmP-P] was measured using the vibration module. In addition, an agitation experiment was performed at a frequency where the vibration mode occurs.

  Specifically, three anti-vibration insulators were disposed below the vibration module, and the vibration module was supported at three points. Further, a laser displacement meter was disposed at an upper position facing the center of the upper surface of the vibration module, and the displacement of the vibration, that is, the amplitude was measured by the laser displacement meter. The measurement was performed when the chip cassette 12 was not attached and when the chip cassette 12 was attached. When the chip cassette 12 was attached, a pole as an example of a rod-shaped member corresponding to the extension cylinder 3 was fixed to the center of the upper surface of the vibration module, and the holder and the chip cassette were supported at the tip of the pole.

FIG. 3 is an explanatory diagram of Experimental Example 1, and is an explanatory diagram of measurement results of frequency and amplitude.
As indicated by the solid line in FIG. 3, when the chip cassette 12 was not attached to the vibration module, a large amplitude was generated when the frequency was 15 [Hz], and the primary mode was confirmed. Further, when the frequency was 60 [Hz], a large amplitude was generated, and the secondary mode was confirmed.
On the other hand, when the chip cassette 12 was attached to the vibration module, as shown by the one-dot chain line in FIG. 3, a large amplitude occurred when the frequency was 15 [Hz], and the primary mode was confirmed. A large amplitude was confirmed when the frequency was 52 [Hz], and the secondary mode was confirmed. Therefore, it was confirmed that when the chip cassette 12 was attached and the mass of the vibration system increased, the resonance frequency of the low-order secondary mode shifted in the direction of decreasing the frequency. In addition, it was confirmed that the resonance frequency 15 [Hz] of the primary mode hardly shifted before and after the chip cassette 12 was attached. Therefore, the resonance frequency 15 [Hz] in the primary mode is considered to be a resonance frequency corresponding to the resonance frequency of the vibration module, not the chip cassette 12, because the frequency is hardly shifted.

Next, a stirring experiment was performed by controlling the vibration module based on the frequency of the specified vibration mode. In the agitation experiment, 100 [μL] pure water was injected into the chip cassette 12 and several drops of paint were dropped as a marker.
When the vibration module was controlled while modulating in the range of 47 to 57 [Hz] corresponding to the resonance frequency of 52 [Hz] in the secondary mode, it was agitated in 10 seconds or less. Further, when the same stirring experiment was performed with the amount of pure water set to 70 [μL], stirring was performed in 10 seconds or less. At this time, it also vibrated in the twisting direction. However, when an agitation experiment was performed in a frequency range based on the resonance frequency 15 [Hz] of the primary mode, the flow of the liquid could not be confirmed in the chip cassette, and the agitation was not achieved. Here, the resonance frequency 15 [Hz] of the primary mode is a resonance frequency corresponding to the resonance frequency of the vibration module. Therefore, in the primary mode, the vibration of the vibration module becomes dominant and the chip cassette 12 resonates. It is considered that the liquid did not reach stirring because of difficulty.

(Experimental example 2)
FIG. 4 is an explanatory diagram of Experimental Example 2 and Experimental Example 3. FIG. 4A is an explanatory diagram of the arrangement position of the chip cassette of Experimental Example 2. FIG. 4B is an explanatory diagram when the chip cassette is vibrated by point contact. 4C is an explanatory diagram for comparison when the chip cassette is vibrated by surface contact, and FIG. 4D is an explanatory diagram for the arrangement position of the chip cassette of Experimental Example 3. FIG.
4A and 4B, in Experimental Example 2, as in Experimental Example 1, a vibration module having a diameter of 115φ [mm] and a rated maximum input of 20 [W] was used. Further, the chip cassette 12 is fixed above the thin aluminum plate 13 ', and the vibration from the vibration module is transmitted to the center of the lower surface of the aluminum plate 13' by point contact. In Experimental Example 2, the amplitude input to the vibration module was confirmed using an oscilloscope.

  4A and 4B, in Experimental Example 2, 100 [μL] pure water L1 was injected into the chip cassette 12. In addition, 1 to 2 drops of paint were dropped as the marker L2 in the pure water L1. Then, the vibration module was controlled to vibrate. At this time, the amplitude is increased from the start of stirring (0 [Vpp]) to 10 [Vpp] at a rise time Tr = 10 [seconds], and from 10 [Vpp] to 20 [Vpp], the rise time Tr = Raised in 2 [seconds]. During the subsequent stirring, the amplitude was maintained at 20 [Vpp]. At this time, the frequency was modulated in a preset frequency range of 47 to 57 [Hz].

  In FIG. 4B, it was confirmed that the marker L2 spread over the entire pure water L1 and the entire pure water L1 and the marker L2 were stirred 20 to 30 seconds after the input of the vibration module was started. Here, when the amplitude is increased at a stroke from the start of stirring (0 [Vpp]) to the target amplitude of 20 [Vpp] that can be stirred at the rise time Tr = 10 [seconds], the pure water L1 The problem of jumping up and scattering occurred. Further, when the maximum amplitude was 10 [Vpp], the agitation was not performed. Note that an output with an amplitude exceeding 20 [Vpp] exceeds the rating of the VCM drive circuit and has not been confirmed, but is presumed to be stirred without any problem.

  In addition, an experiment for comparison with Experimental Example 2 was also performed. The comparative experiment was performed in the same manner as in Experimental Example 2 except that the vibration from the vibration module was transmitted to the lower surface of the aluminum plate 13 ′ by surface contact. In FIG. 4C, in the case of surface contact, it was observed that the marker L2 did not spread over the entire pure water L1, and a portion where the marker L2 was mixed moved in the chip cassette in a lump. That is, it was confirmed that poor agitation occurs in the surface contact.

(Experimental example 3)
4D, in Experimental Example 3, a voice coil motor having a diameter of 20 [mm] and a rated input of 20 [W] was used. Four chip cassettes 12 are arranged on the thin aluminum plate 13 ″. Further, the configuration is such that the vibration from the voice coil motor is transmitted to the center of the lower surface of the aluminum plate 13 ″ by point contact.
In Experimental Example 3, 100 [μL] pure water L1 was injected into each chip cassette 12. In addition, 1 to 2 drops of paint were dropped as the marker L2 in the pure water L1. The voice coil motor was controlled to vibrate. At this time, the amplitude is increased from the start of stirring (0 [Vpp]) to 5 [Vpp] with a rise time Tr = 10 [seconds], and from 5 [Vpp] to 10 [Vpp], the rise time Tr = Raised in 2 [seconds]. During the subsequent stirring, the amplitude was maintained at 10 [Vpp]. Except for these points, when stirring was performed in the same manner as in Experimental Example 2, it was confirmed that the entire pure water L1 and the marker L2 were stirred in each chip cassette. Therefore, it was confirmed that a plurality of chip cassettes can be stirred at the same time.

(Example of change)
As mentioned above, although the Example of this invention was explained in full detail, this invention is not limited to the said Example, A various change is performed within the range of the summary of this invention described in the claim. It is possible. Modification examples (H01) to (H08) of the present invention are exemplified below.
(H01) In the above-described embodiment, the solution stirring apparatus 1 has exemplified the configuration of the chip cassette 12 as a container in which the liquid L to be stirred is stirred. However, the present invention is not limited to this. For example, it is possible to stir using only the cassette body 14 from which the cover 18 is omitted from the chip cassette 12. Further, it is also possible to perform stirring using only the bottom portion 15 and the stirring portion 16 in which the container wall 17 is further omitted from the cassette body 14. Furthermore, the structure which supports, accommodates, and stirs the to-be-stirred liquid L with the plate-shaped member whose depth h is 0 like a slide glass is also possible. For example, when the liquid to be stirred L is stirred on a slide glass, it can be observed with a microscope or the like without transferring the liquid after stirring.

(H02) In the above embodiment, the configuration in which the voice coil motor is controlled in the frequency range A including the resonance frequency of the secondary mode as an example of the low-order mode is exemplified, but the present invention is not limited to this. If the vibration mode is a frequency range A including the resonance frequency generated in the vibration part 11, the configuration of modulating the frequency of the voice coil motor 2 in an arbitrary frequency range A based on the resonance frequency of the vibration part 11 is possible. .
(H03) In the above-described embodiment, the vibration part 11 has a configuration in which one chip cassette 12 is supported with respect to the holder 13, but the present invention is not limited to this. As a configuration in which the plurality of chip cassettes 12 are supported with respect to the holder 13, a configuration in which the plurality of chip cassettes 12 are stirred at the same time is possible.

(H04) In the above-described embodiment, the temperature control unit 21 is supported by the holder 13 or exemplified as a configuration fixed to the holder 13, but the present invention is not limited to this. A heating element may be provided on the lower surface of the chip cassette 12 and an electrode that contacts the heating element may be provided on the holder 13 so that the chip cassette 12 is heated by supplying power from the holder 13.
(H05) In the above-described embodiment, the contact portion 3b on the upper portion of the extension cylinder 3 is formed in a cone shape and is in point contact. However, the present invention is not limited to this. Any convex shape is possible.

(H06) In the above embodiment, the configuration of the solution stirring apparatus 1 in which the voice coil motor 2 and the holder 13 are integrally assembled is illustrated, but the present invention is not limited to this. For example, the solution agitator 1 can be configured as an automatic analyzer. That is, the holder 13 is configured to hold and transfer the chip cassette 12 in the automatic analyzer. And when the holder 13 moves to the arrangement position of the voice coil motor 2, the voice coil motor 2, the extension cylinder 3 and the like are brought close to and brought into contact with the holder 13, and vibration is transmitted to the holder 13 so that the liquid L to be stirred is supplied. A configuration in which stirring is performed is possible. After stirring, the extension cylinder 3 and the like can be withdrawn from the holder 13, and the chip cassette after stirring can be further transferred by the holder 13 from which the extension cylinder 3 and the like are separated. Here, the extension cylinder 3 is formed so as to protrude upward, and can make point contact with the holder 13. Therefore, for example, even if the extension cylinder 3 is inclined due to attachment error or rattling when moving closer, the contact position is less likely to be displaced than in the case of surface contact, and vibration occurs at a stable position with respect to the holder 13 or the like. Easy to be transmitted.

(H07) In the above-described embodiments, the specific numerical values illustrated are changed to arbitrary numerical values in accordance with the difference in use in designing and designing the configuration and size of the device within the range where the operation and effect of the present invention are achieved. Is possible.
(H08) In the above-described embodiment, the configuration of the extension cylinder 3 is exemplified as the vibration transmission unit, but the present invention is not limited to this. For example, a rod-shaped member, a so-called pole, can be fixed to the center of the upper portion of the voice coil motor, and the vibration can be transmitted to the vibrating portion 11 via the pole.

1 ... Solution stirring device,
2 ... Voice coil motor,
3 ... vibration transmission part,
11 ... Vibrated part,
16 ... the accommodating part,
C1 ... control means,
L: Liquid to be stirred.

Claims (3)

  1. A to-be-vibrated portion having a containing portion in which an amount of liquid to be stirred of 200 μL or less is contained and has a depth of 1.5 mm or less;
    A voice coil motor that vibrates along the vertical direction and imparts vibration to stir the liquid to be stirred, with the force that the coil through which the current flows receives by the magnetic field as an operating principle;
    A vibration transmitting unit that is supported by the voice coil motor and configured to be capable of point contact with the vibrating unit, and transmits the vibration of the voice coil motor to the housing unit;
    Control means for oscillating the voice coil motor, the control means for oscillating the voice coil motor while changing the frequency in a predetermined frequency range around the resonance frequency of the oscillated portion;
    A solution agitation apparatus comprising:
  2. The control means for vibrating the voice coil motor while changing in the frequency range based on the primary or secondary resonance frequency of the vibration part;
    The solution stirring apparatus according to claim 1, comprising:
  3. When stirring the liquid to be stirred, a vibration with a first amplitude set in advance is applied, and when the voice coil motor is started, the first amplitude is reduced to a second amplitude smaller than the first amplitude. The control means for raising the first rise time from the second amplitude to the first amplitude at a second rise time shorter than the first rise time after being raised at the rise time of one;
    The solution stirring apparatus according to claim 1, comprising:
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JPS5839926A (en) * 1981-09-03 1983-03-08 Asahi Glass Co Ltd Analyzer
US4610546A (en) * 1984-12-31 1986-09-09 Technicon Instruments Corporation Apparatus and method for self-resonant vibrational mixing
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JP2001327846A (en) * 2000-05-24 2001-11-27 Naoyuki Aoyama Method for agitating fine liquid droplet and device used in the method
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