JP6506768B2 - 多重アプタマー標的の検出 - Google Patents
多重アプタマー標的の検出 Download PDFInfo
- Publication number
- JP6506768B2 JP6506768B2 JP2016544355A JP2016544355A JP6506768B2 JP 6506768 B2 JP6506768 B2 JP 6506768B2 JP 2016544355 A JP2016544355 A JP 2016544355A JP 2016544355 A JP2016544355 A JP 2016544355A JP 6506768 B2 JP6506768 B2 JP 6506768B2
- Authority
- JP
- Japan
- Prior art keywords
- aptamer
- deoxyuridine
- target
- complex
- carboxamide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/33—Chemical structure of the base
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/205—Aptamer
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Plural Heterocyclic Compounds (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361881629P | 2013-09-24 | 2013-09-24 | |
| US61/881,629 | 2013-09-24 | ||
| PCT/US2014/057143 WO2015048084A1 (en) | 2013-09-24 | 2014-09-24 | Multiaptamer target detection |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016531593A JP2016531593A (ja) | 2016-10-13 |
| JP2016531593A5 JP2016531593A5 (https=) | 2017-11-02 |
| JP6506768B2 true JP6506768B2 (ja) | 2019-04-24 |
Family
ID=52744404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016544355A Active JP6506768B2 (ja) | 2013-09-24 | 2014-09-24 | 多重アプタマー標的の検出 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US9926566B2 (https=) |
| EP (1) | EP3049523B1 (https=) |
| JP (1) | JP6506768B2 (https=) |
| KR (1) | KR102348283B1 (https=) |
| CN (2) | CN114703193A (https=) |
| AU (1) | AU2014326975B2 (https=) |
| BR (1) | BR112016005957A2 (https=) |
| CA (1) | CA2924987C (https=) |
| HK (1) | HK1218765A1 (https=) |
| IL (1) | IL244284B (https=) |
| MX (1) | MX361451B (https=) |
| SG (1) | SG11201602063UA (https=) |
| WO (1) | WO2015048084A1 (https=) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6506768B2 (ja) | 2013-09-24 | 2019-04-24 | ソマロジック・インコーポレーテッド | 多重アプタマー標的の検出 |
| TWI695068B (zh) | 2014-11-24 | 2020-06-01 | 美商身體邏輯股份有限公司 | 用於結合生長分化因子１１的核酸化合物 |
| PL3387152T3 (pl) * | 2015-12-08 | 2022-05-09 | Twinstrand Biosciences, Inc. | Ulepszone adaptory, sposoby i kompozycje do sekwencjonowania dupleksowego |
| JP6853265B2 (ja) | 2016-03-14 | 2021-03-31 | ソマロジック, インコーポレイテッドSomaLogic, Inc. | 核酸に組み込むための５−（ｎ−保護−トリプタミノカルボキシアミド）−２’−デオキシウリジンホスホロアミダイトの化合物及び合成方法 |
| KR20190017870A (ko) * | 2016-06-17 | 2019-02-20 | 프록시머티 바이오사이언시스, 엘엘씨 | 앱타머 쌍 선택 방법 |
| TWI770036B (zh) | 2016-07-01 | 2022-07-11 | 美商私募蛋白質體操作有限公司 | 包含經修飾核苷之寡核苷酸 |
| DK3548652T3 (da) | 2016-12-01 | 2024-07-08 | Nautilus Subsidiary Inc | Fremgangsmåder til proteinanalyse |
| WO2019103131A1 (ja) * | 2017-11-24 | 2019-05-31 | 国立大学法人 東京大学 | アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法 |
| KR20250152680A (ko) | 2017-12-29 | 2025-10-23 | 노틸러스 서브시디어리, 인크. | 단백질 식별을 위한 디코딩 접근법 |
| EP3810563A4 (en) * | 2018-06-22 | 2022-03-23 | Somalogic, Inc. | IMPROVED PROTEOMIC MULTIPLEX TESTS |
| AU2021288692A1 (en) | 2020-06-11 | 2023-02-02 | Nautilus Subsidiary, Inc. | Methods and systems for computational decoding of biological, chemical, and physical entities |
| EP4281774B1 (en) | 2021-01-20 | 2025-11-26 | Nautilus Subsidiary, Inc. | Biomolecule quantitation |
| EP4281775B1 (en) | 2021-01-21 | 2026-04-29 | Nautilus Subsidiary, Inc. | Systems and methods for biomolecule preparation |
| IL305336B2 (en) | 2021-03-11 | 2025-09-01 | Nautilus Subsidiary Inc | Systems and methods for preserving biomolecules |
| CA3227872A1 (en) | 2021-09-09 | 2023-03-16 | James Henry JOLY | Characterization and localization of protein modifications |
| CA3227592A1 (en) | 2021-09-22 | 2023-03-30 | Gregory KAPP | Methods and systems for determining polypeptide interactions |
| US20230149883A1 (en) | 2021-11-03 | 2023-05-18 | Nautilus Biotechnology, Inc. | Systems and methods for surface structuring |
| US12092578B2 (en) | 2022-03-29 | 2024-09-17 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
| WO2023212490A1 (en) | 2022-04-25 | 2023-11-02 | Nautilus Subsidiary, Inc. | Systems and methods for assessing and improving the quality of multiplex molecular assays |
| EP4544077A1 (en) | 2022-06-21 | 2025-04-30 | Nautilus Subsidiary, Inc. | Method for detecting analytes at sites of optically non-resolvable distances |
| WO2024059655A1 (en) | 2022-09-15 | 2024-03-21 | Nautilus Subsidiary, Inc. | Characterizing accessibility of macromolecule structures |
| WO2024073599A1 (en) | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
| WO2024107857A1 (en) | 2022-11-15 | 2024-05-23 | Nautilus Subsidiary, Inc. | Standard polypeptides |
| WO2024124073A1 (en) | 2022-12-09 | 2024-06-13 | Nautilus Subsidiary, Inc. | A method comprising performing on a single-analyte array at least 50 cycles of a process |
| US20240201182A1 (en) | 2022-12-15 | 2024-06-20 | Nautilus Subsidiary, Inc. | Inhibition of photon phenomena on single molecule arrays |
| US20240241110A1 (en) | 2023-01-12 | 2024-07-18 | Nautilus Subsidiary, Inc. | Characterization of glycans and glycoconjugates |
| EP4689173A1 (en) | 2023-03-24 | 2026-02-11 | Nautilus Subsidiary, Inc. | Improved transfer of nanoparticles to array surfaces |
| WO2024216233A1 (en) | 2023-04-13 | 2024-10-17 | Nautilus Subsidiary, Inc. | Artificial proteins for displaying epitopes |
| US20240394902A1 (en) | 2023-05-24 | 2024-11-28 | Nautilus Subsidiary, Inc. | Systems and methods for machine vision |
| US20250189519A1 (en) | 2023-12-11 | 2025-06-12 | Nautilus Subsidiary, Inc. | Methods and compositions of particle-based arrays |
| WO2025137166A1 (en) | 2023-12-20 | 2025-06-26 | Nautilus Subsidiary, Inc. | Fluidic devices for single-analyte assays |
| US20250298016A1 (en) | 2024-03-22 | 2025-09-25 | Nautilus Subsidiary, Inc. | Plasmonic particle systems for single-analyte assays |
| WO2025212338A1 (en) | 2024-04-01 | 2025-10-09 | Nautilus Subsidiary, Inc. | Nanostructures for modulation of analyte conformation |
| WO2026073213A1 (en) * | 2024-09-30 | 2026-04-02 | Illumina, Inc. | Protein capture techniques with surface linked aptamers |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020037506A1 (en) * | 2000-04-18 | 2002-03-28 | Yun Lin | Aptamer based two-site binding assay |
| US7312325B2 (en) * | 2000-09-26 | 2007-12-25 | Duke University | RNA aptamers and methods for identifying the same |
| US20070071744A1 (en) * | 2002-06-07 | 2007-03-29 | Gotz Munch | Agents which bind to epitopes of glycoprotein VI |
| US7595160B2 (en) | 2004-01-13 | 2009-09-29 | U.S. Genomics, Inc. | Analyte detection using barcoded polymers |
| US7795009B2 (en) * | 2005-06-15 | 2010-09-14 | Saint Louis University | Three-component biosensors for detecting macromolecules and other analytes |
| US20100035247A1 (en) | 2005-11-04 | 2010-02-11 | U.S. Genomics, Inc. | Heterogeneous Assay of Analytes in Solution Using Polymers |
| US8658670B2 (en) | 2006-09-26 | 2014-02-25 | Crestone, Inc. | Methods and compounds for treatment of clostridium based infection |
| US7947447B2 (en) | 2007-01-16 | 2011-05-24 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| DK2172566T4 (da) * | 2007-07-17 | 2022-06-13 | Somalogic Inc | Fremgangsmåde til generering af aptamerer med forbedrede off-rates |
| WO2009037264A2 (en) | 2007-09-17 | 2009-03-26 | Da Volterra | Colonic delivery of antimicrobial agents |
| US8569252B2 (en) | 2009-04-15 | 2013-10-29 | Postech Academy-Industry Foundation | Nucleolin specific aptamer and use thereof |
| WO2011100443A1 (en) | 2010-02-11 | 2011-08-18 | Intelligent Medical Devices, Inc. | Oligonucleotides relating to clostridium difficile genes encoding toxin b, toxin a, or binary toxin |
| GB201002627D0 (en) | 2010-02-16 | 2010-03-31 | Loxbridge Res Llp | Aptamer based analyte detection method |
| EP3345915B1 (en) * | 2010-04-12 | 2021-11-24 | Somalogic, Inc. | 5-position modified pyrimidines and their use |
| US8785132B2 (en) | 2010-04-23 | 2014-07-22 | Postech Academy-Industry Foundation | Aptamer sandwich assays |
| AU2012225273B2 (en) | 2011-03-10 | 2016-10-13 | Somalogic, Inc. | Aptamers for Clostridium difficile diagnostics |
| US20150031585A1 (en) | 2011-03-10 | 2015-01-29 | Somalogic, Inc. | Multiaptamer Target Detection |
| KR20140044324A (ko) * | 2011-04-26 | 2014-04-14 | 레가도 바이오사이언스, 인코포레이티드 | 페길화된 올리고뉴클레오티드의 제조방법 |
| MX373248B (es) | 2011-09-30 | 2020-05-11 | Somalogic Operating Co Inc | Prediccion de riesgo de evento cardiovascular y usos del mismo. |
| EP2798085B1 (en) | 2011-12-30 | 2018-01-24 | Quest Diagnostics Investments Incorporated | Aptamers and diagnostic methods for detecting the egf receptor |
| JP6506768B2 (ja) | 2013-09-24 | 2019-04-24 | ソマロジック・インコーポレーテッド | 多重アプタマー標的の検出 |
-
2014
- 2014-09-24 JP JP2016544355A patent/JP6506768B2/ja active Active
- 2014-09-24 MX MX2016003320A patent/MX361451B/es active IP Right Grant
- 2014-09-24 CA CA2924987A patent/CA2924987C/en active Active
- 2014-09-24 CN CN202210264586.7A patent/CN114703193A/zh active Pending
- 2014-09-24 SG SG11201602063UA patent/SG11201602063UA/en unknown
- 2014-09-24 AU AU2014326975A patent/AU2014326975B2/en active Active
- 2014-09-24 HK HK16106780.3A patent/HK1218765A1/zh unknown
- 2014-09-24 BR BR112016005957A patent/BR112016005957A2/pt not_active IP Right Cessation
- 2014-09-24 US US15/023,182 patent/US9926566B2/en active Active
- 2014-09-24 CN CN201480051210.1A patent/CN105555957A/zh active Pending
- 2014-09-24 WO PCT/US2014/057143 patent/WO2015048084A1/en not_active Ceased
- 2014-09-24 KR KR1020167006262A patent/KR102348283B1/ko active Active
- 2014-09-24 EP EP14847780.5A patent/EP3049523B1/en active Active
-
2016
- 2016-02-25 IL IL244284A patent/IL244284B/en active IP Right Grant
-
2018
- 2018-02-13 US US15/895,723 patent/US10392621B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3049523B1 (en) | 2019-08-07 |
| CA2924987C (en) | 2022-08-16 |
| MX2016003320A (es) | 2016-05-31 |
| IL244284B (en) | 2019-12-31 |
| HK1218765A1 (zh) | 2017-03-10 |
| IL244284A0 (en) | 2016-04-21 |
| MX361451B (es) | 2018-12-06 |
| KR102348283B1 (ko) | 2022-01-10 |
| EP3049523A1 (en) | 2016-08-03 |
| CN114703193A (zh) | 2022-07-05 |
| US20160237435A1 (en) | 2016-08-18 |
| US20180187197A1 (en) | 2018-07-05 |
| AU2014326975A1 (en) | 2016-04-07 |
| US10392621B2 (en) | 2019-08-27 |
| BR112016005957A2 (pt) | 2017-09-26 |
| WO2015048084A1 (en) | 2015-04-02 |
| AU2014326975A2 (en) | 2017-01-05 |
| KR20160058777A (ko) | 2016-05-25 |
| CA2924987A1 (en) | 2015-04-02 |
| SG11201602063UA (en) | 2016-04-28 |
| EP3049523A4 (en) | 2017-05-17 |
| JP2016531593A (ja) | 2016-10-13 |
| US9926566B2 (en) | 2018-03-27 |
| CN105555957A (zh) | 2016-05-04 |
| AU2014326975B2 (en) | 2020-05-07 |
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