JP5925345B2 - Salty taste enhancer - Google Patents
Salty taste enhancer Download PDFInfo
- Publication number
- JP5925345B2 JP5925345B2 JP2015019829A JP2015019829A JP5925345B2 JP 5925345 B2 JP5925345 B2 JP 5925345B2 JP 2015019829 A JP2015019829 A JP 2015019829A JP 2015019829 A JP2015019829 A JP 2015019829A JP 5925345 B2 JP5925345 B2 JP 5925345B2
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- JP
- Japan
- Prior art keywords
- salty taste
- salt
- acid
- protein
- amino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/40—Table salts; Dietetic salt substitutes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
- A23L27/201—Compounds of unspecified constitution characterised by the chemical reaction for their preparation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/86—Addition of bitterness inhibitors
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Seasonings (AREA)
- Seeds, Soups, And Other Foods (AREA)
- Peptides Or Proteins (AREA)
Description
本発明は、食塩味増強剤および食塩味増強方法に関する。 The present invention relates to a salty taste enhancer and a salty taste enhancing method.
食塩(塩化ナトリウム)は、飲食品の調味や加工において、古来より広く使用される調味料であり、人体に必須の栄養成分からなることもあり、我々の生活にはなくてはならない調味料である。 Salt (sodium chloride) is a seasoning that has been widely used in the seasoning and processing of foods and drinks, and it is an essential seasoning for our daily life. is there.
しかし、その一方で、ナトリウムは、その過剰摂取により高血圧などの症状を示すことが示唆されており、また、脳卒中などの循環器系疾患の発症リスクを高めるといわれている。 On the other hand, sodium has been suggested to exhibit symptoms such as hypertension due to its overdose, and is said to increase the risk of developing cardiovascular diseases such as stroke.
日本における1日あたりの食塩摂取量は国の目標値を超え、慢性的な食塩過剰摂取の傾向にあるため対応が必要とされているが、単に食品中の食塩含有量を低減させた場合、特に10%以上低減させた場合は、食塩による塩味の減少とともに、該飲食品に必要とされる風味も低下する、すなわち食塩味が低下することがあり、何らかの工夫が必要である。 The daily salt intake in Japan exceeds the national target, and there is a tendency for chronic excessive salt intake, so it is necessary to take action, but if you simply reduce the salt content in food, In particular, when it is reduced by 10% or more, the salty taste due to the salt is reduced and the flavor required for the food or drink is also lowered, that is, the salty taste may be lowered, and some contrivance is necessary.
食塩の含有量を低減させた飲食品の食塩の代替として、食塩のかわりに塩化カリウムおよび糖アルコール(特許文献1)、トレハロース(特許文献2)等の、それ自身が食塩味を有する成分(以下、食塩代替成分ともいう)や、酸性ペプチド(特許文献3)、酸性アミノ酸、塩基性アミノ酸およびコハク酸(特許文献4)、黒麹や黄麹の混合物を消化分解して得られる分解液(特許文献5)、セチルピリジニウム塩またはセチルピリジニウム塩と塩基性アミノ酸との混合物(特許文献6)等の、それ自身は食塩味をほとんど有さないが食塩と共存することで、食塩味を増強する成分(以下、食塩味増強成分ともいう)等が添加されることがあるが、これらの添加では、低下した塩味や風味の補完が不十分であったり、また、風味に悪影響を及ぼしたりする場合があることから、新たな食塩味の補完方法の開発が望まれている。 As an alternative to salt in foods and beverages with reduced salt content, components that themselves have a salty taste, such as potassium chloride and sugar alcohol (Patent Document 1), trehalose (Patent Document 2), etc. , A salt substitute component), an acidic peptide (Patent Document 3), an acidic amino acid, a basic amino acid and succinic acid (Patent Document 4), a decomposition solution obtained by digesting and decomposing a mixture of black candy and jaundice (patent Ingredients that enhance saltiness by coexisting with salt, such as literature 5), cetylpyridinium salt, or a mixture of cetylpyridinium salt and basic amino acid (Patent Document 6), which itself has little salty taste. (Hereinafter also referred to as a salty taste enhancing component) and the like may be added. However, these additions may not be sufficient to supplement the reduced salty taste or flavor, and may adversely affect the flavor. Since the case is to be or has been desired to develop a complementary method of new salty taste.
本発明の目的は、飲食品の風味を損なうことなく、飲食品の食塩の含有量を低減できる食塩味増強剤および食塩味増強方法を提供することにある。 An object of the present invention is to provide a salty taste enhancer and a salty taste enhancing method capable of reducing the salt content of a food or drink without impairing the flavor of the food or drink.
本発明は、以下の(1)〜(12)に関する。 The present invention relates to the following (1) to (12).
(1)ペプチドとカルボニル化合物とのアミノ−カルボニル反応物および有機酸を含有することを特徴とする食塩味増強剤。 (1) A salty taste enhancer comprising an amino-carbonyl reaction product of a peptide and a carbonyl compound and an organic acid.
(2)アミノ−カルボニル反応物が、分子量1000〜5000のペプチドとカルボニル化合物とのアミノ−カルボニル反応物である、上記(1)の食塩味増強剤。 (2) The salty taste enhancer according to (1) above, wherein the amino-carbonyl reactant is an amino-carbonyl reactant of a peptide having a molecular weight of 1000 to 5000 and a carbonyl compound.
(3)有機酸が、コハク酸またはリンゴ酸である、上記(1)または(2)の食塩味増強剤。 (3) The salty taste enhancer according to (1) or (2) above, wherein the organic acid is succinic acid or malic acid.
(4)ペプチドとカルボニル化合物とのアミノ−カルボニル反応物および有機酸を、食塩を含有する飲食品に添加することを特徴とする、食塩を含有する飲食品の食塩味増強方法。 (4) A method for enhancing the salty taste of a food or drink containing salt, comprising adding an amino-carbonyl reaction product of a peptide and a carbonyl compound and an organic acid to the food or drink containing salt.
(5)アミノ−カルボニル反応物が、分子量1000〜5000のペプチドとカルボニル化合物とのアミノ−カルボニル反応物である、上記(4)の方法。 (5) The method according to (4) above, wherein the amino-carbonyl reactant is an amino-carbonyl reactant of a peptide having a molecular weight of 1000 to 5000 and a carbonyl compound.
(6)有機酸が、コハク酸またはリンゴ酸である、上記(4)または(5)の方法。 (6) The method of (4) or (5) above, wherein the organic acid is succinic acid or malic acid.
(7)食塩を含有する飲食品が、減塩飲食品である、上記(4)〜(6)いずれか1つの方法。 (7) The method according to any one of (4) to (6) above, wherein the food or drink containing salt is a reduced salt food or drink.
(8)上記(1)〜(3)いずれか1つの食塩味増強剤を、食塩を含有する飲食品に添加することを特徴とする、食塩を含有する飲食品の食塩味増強方法。 (8) The salty taste enhancing method for foods and drinks containing salt, characterized in that any one of the salty taste enhancers (1) to (3) is added to foods and drinks containing salt.
(9)食塩を含有する飲食品が、減塩飲食品である、上記(8)の方法。 (9) The method of said (8) that the food / beverage products containing salt are low salt food / beverage products.
(10)ペプチドとカルボニル化合物とのアミノ−カルボニル反応物および有機酸を、食塩を含有する飲食品に添加することを特徴とする飲食品の製造方法。 (10) A method for producing a food or drink comprising adding an amino-carbonyl reaction product of a peptide and a carbonyl compound and an organic acid to the food or drink containing sodium chloride.
(11)上記(1)〜(3)いずれか1つの食塩味増強剤を、食塩を含有する飲食品に添加することを特徴とする、飲食品の製造方法。 (11) A method for producing a food or drink, comprising adding any one of the salt taste enhancers (1) to (3) to a food or drink containing salt.
(12)食塩を含有する飲食品が、減塩飲食品である、上記(10)または(11)の方法。 (12) The method according to (10) or (11) above, wherein the food or drink containing salt is a reduced salt food or drink.
本願は、2009年3月16日に出願された日本国特許出願2009-62292号の優先権を主張するものであり、該特許出願の明細書に記載される内容を包含する。 This application claims the priority of the Japan patent application 2009-62292 for which it applied on March 16, 2009, and includes the content described in the specification of this patent application.
本発明により、飲食品の風味を損なうことなく、飲食品の食塩の含有量を低減できる食塩味増強剤および食塩味増強方法を提供することができる。 According to the present invention, it is possible to provide a salty taste enhancer and a salty taste enhancing method capable of reducing the salt content of a food or drink without impairing the flavor of the food or drink.
本発明の食塩味増強剤を、例えば食塩の含有量を低減させた飲食品に添加することにより製造された飲食品は、食塩の含有量が低いにも関わらず、塩味を強く感じることができ、かつ風味もよい。 The food and drink produced by adding the salty taste enhancer of the present invention to a food and drink with reduced salt content, for example, can feel a strong salty taste even though the salt content is low. And the flavor is good.
本発明に用いられるペプチドとカルボニル化合物とのアミノ−カルボニル反応物(以下、単にアミノ−カルボニル反応物ともいう)の調製に用いられるペプチドは、ペプチド合成により得たものでもよいが、蛋白質を蛋白質分解酵素、酸等を用いて加水分解して得られる蛋白質加水分解物、該蛋白質加水分解物を限外ろ過、ゲルろ過等の分子量分画方法に供し、分取して得られるペプチドを含有する画分等のペプチド含有物等を用いてもよい。 The peptide used in the preparation of the amino-carbonyl reaction product (hereinafter also simply referred to as amino-carbonyl reaction product) between the peptide and the carbonyl compound used in the present invention may be obtained by peptide synthesis. A protein hydrolyzate obtained by hydrolysis using an enzyme, acid, etc., and a peptide containing a peptide obtained by subjecting the protein hydrolyzate to a molecular weight fractionation method such as ultrafiltration or gel filtration. Peptide-containing materials such as fractions may be used.
ペプチドを含有する画分としては、たとえば、蛋白質加水分解物を、分子量を指標として分画して得られる、分子量500以上、好ましくは1000〜20,000、より好ましくは1000〜10,000、さらに好ましくは分子量1000〜5000の画分が用いられる。 As a fraction containing a peptide, for example, a molecular weight of 500 or more, preferably 1000 to 20,000, more preferably 1000 to 10,000, obtained by fractionating a protein hydrolyzate with the molecular weight as an index, A fraction having a molecular weight of 1000 to 5000 is preferably used.
蛋白質加水分解物は、動物蛋白質加水分解物(HAP、EAP等)、植物蛋白質加水分解物(HVP、EVP等)、酵母エキス等として市販されているものを用いてもよく、大豆蛋白質、小麦蛋白質、トウモロコシ蛋白質等の植物蛋白質、ホエー蛋白質、カゼイン等の乳蛋白質、卵白蛋白質、卵黄蛋白質等の卵蛋白質、血漿蛋白質、血球蛋白質等の血液蛋白質、食肉蛋白質、魚肉蛋白質等の動物蛋白質、酵母菌体由来の蛋白質を加水分解処理に供して得られるものを用いてもよい。酵母菌体由来の蛋白質としては、ビール酵母、パン酵母、トルラ酵母等の酵母の菌体、および菌体に含まれる蛋白質を単離、精製したもののいずれを用いてもよい。 The protein hydrolyzate may be those commercially available as animal protein hydrolysates (HAP, EAP, etc.), plant protein hydrolysates (HVP, EVP, etc.), yeast extract, etc., soy protein, wheat protein Plant protein such as corn protein, milk protein such as whey protein and casein, egg protein such as egg white protein and egg yolk protein, blood protein such as plasma protein and blood cell protein, animal protein such as meat protein and fish protein, yeast cell A protein obtained by subjecting the derived protein to hydrolysis treatment may be used. As a protein derived from yeast cells, any of yeast cells such as brewer's yeast, baker's yeast, and torula yeast, and proteins isolated and purified from the cells may be used.
蛋白質としては、上記蛋白質に化学処理、酵素処理、物理処理等を施した蛋白質、例えばゼラチン、プラクアルブミン、メタプロテイン、プロテオース、ペプトン等を用いてもよい。ゼラチンとしては、酸処理により得られるゼラチン(タイプA)およびアルカリ処理により得られるゼラチン(タイプB)のいずれを用いてもよい。 As the protein, a protein obtained by subjecting the above protein to chemical treatment, enzyme treatment, physical treatment, etc., for example, gelatin, plac albumin, metaprotein, proteose, peptone or the like may be used. As gelatin, either gelatin obtained by acid treatment (type A) or gelatin obtained by alkali treatment (type B) may be used.
蛋白質は、上記蛋白質のいずれであってもよいが、大豆蛋白質、小麦蛋白質、ホエー蛋白質、カゼイン、血漿蛋白質、卵白蛋白質、ゼラチンまたは酵母菌体由来の蛋白質が好適に用いられる。 The protein may be any of the above-mentioned proteins, but soybean protein, wheat protein, whey protein, casein, plasma protein, egg white protein, gelatin, or a protein derived from yeast cells is preferably used.
蛋白質分解酵素としては、エンドペプチダーゼ(プロテイナーゼともいう)およびエキソペプチダーゼがあげられるが、エンドペプチダーゼを用いることが好ましい。エンドペプチダーゼとしては、エキソペプチダーゼ活性も有するエンドペプチダーゼを用いることが好ましい。エキソペプチダーゼ活性が低いエンドペプチダーゼを用いる場合、エキソペプチダーゼを別途混合して使用してもよい。 Examples of proteolytic enzymes include endopeptidases (also referred to as proteinases) and exopeptidases, but it is preferable to use endopeptidases. As the endopeptidase, an endopeptidase having an exopeptidase activity is preferably used. When an endopeptidase having low exopeptidase activity is used, exopeptidase may be separately mixed and used.
エンドペプチダーゼとしては、例えばトリプシン、キモトリプシン、ズブチリシン等のセリンプロテアーゼ、パパイン、ブロメライン、フィシン等のチオールプロテアーゼ、ペプシン、キモシン等のカルボキシプロテアーゼ、サーモリシン等のメタルプロテアーゼ等があげられる。市販されているエンドペプチダーゼとしては、例えばトリプシン、キモトリプシン、ペプシン、スミチームLP(新日本化学社製)、ビオプラーゼ(ナガセケムテックス社製)、アルカラーゼ(ノボザイムズジャパン社製)等があげられる。 Examples of endopeptidases include serine proteases such as trypsin, chymotrypsin and subtilisin, thiol proteases such as papain, bromelain and ficin, carboxyproteases such as pepsin and chymosin, and metal proteases such as thermolysin. Examples of commercially available endopeptidases include trypsin, chymotrypsin, pepsin, Sumiteam LP (manufactured by Shin Nippon Chemical Co., Ltd.), biopase (manufactured by Nagase ChemteX Corporation), alcalase (manufactured by Novozymes Japan), and the like.
エキソペプチダーゼ活性を有するエンドペプチダーゼとしては、例えばウマミザイム(天野エンザイム社製)、アクチナーゼ(科研ファルマ社製)等があげられる。 Examples of endopeptidases having exopeptidase activity include horseamizyme (manufactured by Amano Enzyme), actinase (manufactured by Kaken Pharma), and the like.
蛋白質分解酵素の使用量は、使用する酵素や蛋白質の種類等によって異なるため特に限定はないが、通常、加水分解処理する蛋白質100重量部に対して0.05〜10重量部である。 The amount of proteolytic enzyme used is not particularly limited because it varies depending on the enzyme used, the type of protein, and the like, but is usually 0.05 to 10 parts by weight with respect to 100 parts by weight of the protein to be hydrolyzed.
蛋白質の加水分解処理のpHや反応温度は、使用する蛋白質分解酵素の最適条件またはそれに近い条件を適宜用いればよい。pHは、塩酸、酢酸、乳酸、クエン酸、リン酸等の飲食品に許容される酸、または水酸化ナトリウム、水酸化カリウム、水酸化カルシウム等の飲食品に許容されるアルカリを添加することによって調整することができる。 The pH and reaction temperature for the protein hydrolysis treatment may be appropriately selected from the optimum conditions of the proteolytic enzyme used or conditions close thereto. The pH is adjusted by adding an acid acceptable for foods and drinks such as hydrochloric acid, acetic acid, lactic acid, citric acid and phosphoric acid, or an alkali acceptable for foods and drinks such as sodium hydroxide, potassium hydroxide and calcium hydroxide. Can be adjusted.
蛋白質の加水分解処理時間は、使用する蛋白質分解酵素の種類、その使用量、温度、pH条件等で異なるが、通常は1〜100時間が好ましく、6〜72時間がより好ましい。 The protein hydrolysis treatment time varies depending on the type of proteolytic enzyme used, the amount used, temperature, pH conditions, etc., but usually 1 to 100 hours are preferable, and 6 to 72 hours are more preferable.
蛋白質を酸により加水分解する場合、蛋白質を、0.1〜1mlo/lの塩酸、硫酸、硝酸等の無機酸、またはクエン酸等の有機酸の存在下で、70〜100℃で、0.5時間〜24時間、好ましくは1時間から5時間加熱処理する。 When the protein is hydrolyzed with an acid, the protein is added at a temperature of 70 to 100 ° C. in the presence of 0.1 to 1 mlo / l of an inorganic acid such as hydrochloric acid, sulfuric acid or nitric acid, or an organic acid such as citric acid. Heat treatment is performed for 5 hours to 24 hours, preferably 1 hour to 5 hours.
蛋白質の加水分解処理後、反応液は、そのまま次の処理に供してもよく、蛋白質分解酵素を用いた場合、加熱処理、酸処理等によって酵素を失活させた後に次の処理に供してもよい。 After the hydrolysis treatment of the protein, the reaction solution may be subjected to the next treatment as it is, or when a proteolytic enzyme is used, it may be subjected to the next treatment after inactivating the enzyme by heat treatment, acid treatment or the like. Good.
ペプチドと共存させるカルボニル化合物としては、カルボニル基をもつ有機化合物であればいずれも用いられるが、好ましくは還元糖、脂質の酸化によって生成するカルボニル化合物が、特に好ましくは還元糖が用いられる。 As the carbonyl compound coexisting with the peptide, any organic compound having a carbonyl group can be used. Preferably, a reducing sugar, a carbonyl compound produced by lipid oxidation, particularly preferably a reducing sugar is used.
還元糖としては、単糖、還元性をもつ二糖以上の多糖類糖があげられる。 Examples of reducing sugars include monosaccharides and polysaccharide sugars having a reducing ability.
単糖としては、トリオース、テトラオース、ペントース、ヘキソース、ヘプトース等があげられ、ペントースまたはヘキソースが好適に用いられる。 Examples of monosaccharides include triose, tetraose, pentose, hexose, heptose and the like, and pentose or hexose is preferably used.
ペントースとしては、L−アラビノース、D−キシロース、D−リボース、D−2−デオキシリボース等があげられ、D−キシロースまたはD−リボースが好適に用いられる。 Examples of the pentose include L-arabinose, D-xylose, D-ribose, D-2-deoxyribose and the like, and D-xylose or D-ribose is preferably used.
ヘキソースとしては、D−グルコース、D−フルクトース、D−ガラクトース、D−マンノース等があげられ、D−グルコースまたはD−フルクトースが好適に用いられる。また、ヘキスロン酸も用いることができ、グルクロン酸、ガラクツロン酸等があげられ、ガラクツロン酸が好適に用いられる。 Examples of hexose include D-glucose, D-fructose, D-galactose, D-mannose, and D-glucose or D-fructose is preferably used. Hexuronic acid can also be used, and examples thereof include glucuronic acid and galacturonic acid, and galacturonic acid is preferably used.
還元糖をもつ二糖以上の多糖類とは、単糖が二個以上結合してできたカルボニル基をもつ多糖類であり、かつアルカリ性でフェーリング溶液を還元するものをいう。例えばマルトース、ラクトース、イソマルトース、マルトトリオース、マルトテトラオース、デキストリン等があげられる。 The polysaccharides having disaccharides or more having a reducing sugar are polysaccharides having a carbonyl group formed by combining two or more monosaccharides, and are alkaline and reduce the Fering solution. Examples thereof include maltose, lactose, isomaltose, maltotriose, maltotetraose, dextrin and the like.
脂質の酸化によって生成するカルボニル化合物としては、脂質の酸化によって生じるハイドロパーオキサイドが分解して生成する飽和アルデヒド、不飽和アルデヒド等のアルデヒド化合物等があげられる。 Examples of the carbonyl compound produced by lipid oxidation include aldehyde compounds such as saturated aldehyde and unsaturated aldehyde produced by decomposition of hydroperoxide produced by lipid oxidation.
飽和アルデヒドとしては、プロパナール、ヘキサナール、オクタナール、ノナナール等があげられ、ヘキサナールまたはノナナールが好適に用いられる。 Examples of the saturated aldehyde include propanal, hexanal, octanal, nonanal and the like, and hexanal or nonanal is preferably used.
不飽和アルデヒドとしては、2−ブテナール(クロトンアルデヒド)、2−ヘキセナール、2−デセナール、2−ウンデセナール、2,4−ヘプタジエナール、2,4−デカジエナール等があげられ、2−ブテナールまたは2−ヘキセナールが好適に用いられる。 Examples of unsaturated aldehydes include 2-butenal (crotonaldehyde), 2-hexenal, 2-decenal, 2-undecenal, 2,4-heptadienal, 2,4-decadienal, and 2-butenal or 2-hexenal. Preferably used.
本発明のアミノ−カルボニル反応物の調製方法を以下に例示する。 The preparation method of the amino-carbonyl reactant of the present invention is exemplified below.
水、または水を主成分とし、他の成分、例えばアルコール、アミノ酸、金属イオン等を含む溶媒中に、ペプチドを1〜60%(w/v)、好ましくは1〜10%(w/v)となるように溶解し、また、カルボニル化合物を0.001〜30%(w/v)、好ましくは0.01〜10%(w/v)となるように溶解して、ペプチドとカルボニル化合物の混合水溶液を調製する。 1 to 60% (w / v), preferably 1 to 10% (w / v) of peptide in water or a solvent containing water as a main component and containing other components such as alcohol, amino acid, metal ion and the like In addition, the carbonyl compound is dissolved in an amount of 0.001 to 30% (w / v), preferably 0.01 to 10% (w / v). A mixed aqueous solution is prepared.
該混合水溶液をpH3〜9、好ましくはpH5〜7に調整し、30〜180℃、好ましくは60〜120℃で、1時間〜数ヶ月、好ましくは1〜6時間反応させることにより、本発明に用いられるアミノ−カルボニル反応物を含有する溶液が調製される。 The mixed aqueous solution is adjusted to pH 3 to 9, preferably pH 5 to 7, and reacted at 30 to 180 ° C, preferably 60 to 120 ° C for 1 hour to several months, preferably 1 to 6 hours. A solution containing the amino-carbonyl reactant used is prepared.
得られたアミノ−カルボニル反応物を含有する溶液は、そのまま本発明の食塩味増強剤の調製に用いてもよいし、必要に応じて活性炭、限外ろ過等による脱色処理、クロマトグラフィー、膜分離等による分離精製処理、減圧濃縮等による濃縮処理などの処理をして、脱色液、精製液、濃縮液等の液体を調製し、これを本発明の食塩味増強剤の調製に用いてもよく、さらに、該液体を減圧乾燥、噴霧乾燥等の乾燥処理などの処理に供して、固形物、粉末等の固体を調製し、これを本発明の食塩味増強剤の調製に用いてもよい。 The resulting solution containing the amino-carbonyl reactant may be used as it is for the preparation of the salty taste enhancer of the present invention, and if necessary, decolorization treatment by activated carbon, ultrafiltration, etc., chromatography, membrane separation. A liquid such as a decolorizing solution, a purified solution, and a concentrated solution may be prepared by performing a separation and purification treatment using a method such as a concentration treatment under reduced pressure, etc., and used for the preparation of the salty taste enhancer of the present invention. Further, the liquid may be subjected to a treatment such as drying under reduced pressure or spray drying to prepare a solid such as a solid or a powder, which may be used for the preparation of the salty taste enhancer of the present invention.
なお、上記アミノ−カルボニル反応物の調製において、ペプチドとカルボニル化合物の混合水溶液のpHを調製後、凍結乾燥、噴霧乾燥等の乾燥処理などを行って得られる乾燥粉末を、相対湿度50〜90%、好ましくは60〜80%に調整し、30〜180℃、好ましくは60〜120℃で、数時間〜数ヶ月間、好ましくは3〜10日間反応させることによっても、アミノ−カルボニル反応物を得ることができる。 In the preparation of the amino-carbonyl reaction product, after adjusting the pH of the mixed aqueous solution of the peptide and the carbonyl compound, a dry powder obtained by performing a drying treatment such as freeze-drying or spray-drying has a relative humidity of 50 to 90%. The amino-carbonyl reactant is also obtained by reacting at 30 to 180 ° C., preferably 60 to 120 ° C., preferably for several hours to several months, preferably for 3 to 10 days. be able to.
本発明において、アミノ−カルボニル反応物とともに用いられる有機酸としては、一般食品用に使用されている有機酸であれば特に限定されないが、アジピン酸、アスコルビン酸、クエン酸、コハク酸、酢酸、シュウ酸、酒石酸、乳酸、リンゴ酸等があげられ、好ましくはコハク酸、アジピン酸またはリンゴ酸があげられ、さらに好ましくはコハク酸またはリンゴ酸があげられる。 In the present invention, the organic acid used together with the amino-carbonyl reactant is not particularly limited as long as it is an organic acid used for general foods, but adipic acid, ascorbic acid, citric acid, succinic acid, acetic acid, oxalic acid. Acid, tartaric acid, lactic acid, malic acid and the like are preferable, succinic acid, adipic acid and malic acid are preferable, and succinic acid and malic acid are more preferable.
本発明の食塩味増強剤は、たとえば、アミノ−カルボニル反応物および有機酸を混合、または水もしくは水を主成分とし他の成分、例えばアルコール、アミノ酸、金属イオン等を含む溶媒中に溶解させて、アミノ−カルボニル反応物および有機酸を含有する組成物として調製することができる。 The salty taste enhancer of the present invention is, for example, mixed with an amino-carbonyl reactant and an organic acid, or dissolved in a solvent containing water or water as a main component, for example, an alcohol, an amino acid, a metal ion or the like. , An amino-carbonyl reactant and an organic acid-containing composition.
有機酸の使用量は、該組成物中の有機酸の含有量が、アミノ−カルボニル反応物100重量部に対して、遊離の有機酸換算で0.1〜50重量部となる量が好ましく、0.5〜30重量部となる量がさらに好ましい。 The amount of the organic acid used is preferably such that the content of the organic acid in the composition is 0.1 to 50 parts by weight in terms of free organic acid with respect to 100 parts by weight of the amino-carbonyl reactant. More preferred is an amount of 0.5 to 30 parts by weight.
本発明の食塩味増強剤は、粉体、粒体、粉粒体等の固体状、溶液等の液体状のいずれの形態であってもよい。 The salty taste enhancer of the present invention may be in the form of a solid such as a powder, a granule or a powder or a liquid such as a solution.
本発明の食塩味増強剤は、アミノ−カルボニル反応物および有機酸以外に、必要に応じて、塩化ナトリウム、塩化カリウム等の無機塩、グルタミン酸ナトリウム、グリシン、アラニン等のアミノ酸、イノシン酸ナトリウム、グアニル酸ナトリウム等の核酸、ショ糖、ブドウ糖、乳糖等の糖、醤油、味噌、畜肉エキス、家禽エキス、魚介エキス、酵母エキス、蛋白質加水分解物等の天然調味料、スパイス類、ハーブ類等の香辛料、デキストリン、各種澱粉等の賦形剤等の飲食品に使用可能な添加物を含有してもよい。食塩(塩化ナトリウム)を含有する本発明の食塩味増強剤は、食塩味調味料として用いてもよい。 The salty taste enhancer of the present invention includes, in addition to the amino-carbonyl reactant and organic acid, an inorganic salt such as sodium chloride and potassium chloride, an amino acid such as sodium glutamate, glycine and alanine, sodium inosinate, and guanyl as necessary. Nucleic acids such as sodium acid, sugars such as sucrose, glucose, and lactose, soy sauce, miso, meat extract, poultry extract, seafood extract, yeast extract, protein hydrolyzate, and other natural seasonings, spices such as spices and herbs Additives that can be used for food and drink such as excipients such as dextrin and various starches. You may use the salty taste enhancer of this invention containing salt (sodium chloride) as a salty seasoning.
本発明の食塩味増強方法としては、本発明の食塩味増強剤を、食塩を含有する飲食品を製造する際に素材の一部として添加する方法、製品となっている食塩を含有する飲食品を加熱調理、電子レンジ調理、真空調理等の調理する際または摂食の際に添加する方法等があげられる。あるいは、本発明の食塩味増強方法としては、前記のアミノ−カルボニル反応物と有機酸とを、食塩を含有する飲食品を製造する際に素材の一部として添加する方法等であってもよい。添加量は、適宜設定すればよい。 As a salty taste enhancing method of the present invention, a method of adding the salty taste enhancing agent of the present invention as a part of a raw material when producing a food or drink containing salt, a food or drink containing salt that is a product And the like, when cooking such as heating cooking, microwave cooking, vacuum cooking, etc. or when eating. Alternatively, the salty taste enhancing method of the present invention may be a method of adding the amino-carbonyl reaction product and the organic acid as a part of the raw material when producing a food or drink containing salt. . What is necessary is just to set the addition amount suitably.
本発明における飲食品は、醤油、めんつゆ、だし汁、煮物調味液、漬物調味液、中華スープ、ドレッシング、味噌、味噌汁、ラーメンスープ(醤油ラーメンスープ、味噌ラーメンスープ等)、塩から、蒲鉾、ちくわ等の水産練加工品、ハム、ソーセージ等の畜肉加工品、和風惣菜、米菓、梅干、佃煮、各種麺、スポーツドリンク、クリーム、バター等の乳製品、コンソメ、ポタージュ等のスープ、トマトソース、ブラウンソース、デミグラスソース、パスタソース等の洋風ソース、各種畜肉エキス、フォン、ブイヨン、和洋中の肉、野菜惣菜、等の食塩を含有するいずれの飲食品であってもよいが、食塩(塩化ナトリウム)の含有量が、これらの飲食品の通常の含有量の10〜90重量%、好ましくは20〜80重量%である飲食品である減塩飲食品が好ましい。 The food and drink in the present invention includes soy sauce, noodle soup, dashi soup, boiled seasoning, pickled seasoning, Chinese soup, dressing, miso, miso soup, ramen soup (soy sauce ramen soup, miso ramen soup, etc.), salt, salmon, chikuwa, etc. Processed fishery products, processed meat products such as ham and sausage, Japanese-style side dishes, rice crackers, plum boiled, boiled sardines, dairy products such as various noodles, sports drinks, cream, butter, soups such as consomme and potage, tomato sauce, brown Any food or beverage containing salt such as sauce, demiglace sauce, pasta sauce, Western-style sauces, various animal meat extracts, von, bouillon, Japanese and Western meats, vegetable side dishes, etc. Reduced content of food and drink with a content of 10 to 90% by weight, preferably 20 to 80% by weight of the normal content of these foods and drinks Food and drink is preferred.
以下、実施例により本発明をさらに具体的に説明する。但し、本発明はこれらに限定されるものではない。 Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to these.
(実施例1)
分離脱脂大豆蛋白質を蛋白質分解酵素で加水分解して得られた分離脱脂大豆蛋白質加水分解物(協和発酵フーズ社製)5gおよび0.12gのn−ヘキサナールを水100mlに分散させ、溶液のpHを6に調整した後、90℃で4時間加熱した。溶液を冷却後、凍結乾燥して粉末1を得た。
Example 1
5 g of a separated defatted soybean protein hydrolyzate (manufactured by Kyowa Hakko Foods) and 0.12 g of n-hexanal obtained by hydrolyzing the separated defatted soybean protein with a proteolytic enzyme are dispersed in 100 ml of water, and the pH of the solution is adjusted. After adjusting to 6, it was heated at 90 ° C. for 4 hours. The solution was cooled and lyophilized to obtain Powder 1.
また、濃い口醤油、だし汁、砂糖、鶏がらスープ、生姜、ニンニク、水等を用いて、常法により塩化ナトリウム濃度1.26重量%の醤油ラーメンスープ(通常タイプ)、および塩化ナトリウム濃度を0.96重量%とした醤油ラーメンスープ(減塩タイプ)を調製した。 Also, using soy sauce ramen soup (normal type) with a sodium chloride concentration of 1.26% by weight and a sodium chloride concentration of 0. A 96% by weight soy sauce ramen soup (low salt type) was prepared.
醤油ラーメンスープ(減塩タイプ)100mlに、粉末1およびコハク酸を、第1表に示すように、単独で、または組み合わせて添加した。 As shown in Table 1, powder 1 and succinic acid were added alone or in combination to 100 ml of soy sauce ramen soup (reduced salt type).
これらの醤油ラーメンスープの中で感じられる塩味、醤油の風味および醤油ラーメンスープとしての好ましさについて、7名のトレーニングされたパネラーにより官能評価を行った。 The sensory evaluation was carried out by seven trained panelists regarding the salty taste, soy sauce flavor, and the preference for soy sauce ramen soup.
評価は、各評価項目において、評価の最も低いものを1点、評価の最も高いものを7点とし、醤油ラーメンスープ(減塩タイプ)を3点とする7点評価法で行った。 In each evaluation item, the evaluation was performed by a 7-point evaluation method in which the lowest evaluation was 1 point, the highest evaluation was 7 points, and soy sauce ramen soup (salted salt type) was 3 points.
結果を第1表に示す。
第1表に示すとおり、醤油ラーメンスープ(減塩タイプ)に、分離脱脂大豆蛋白質加水分解物とn−ヘキサナールとをアミノ−カルボニル反応させて得た粉末1およびコハク酸を添加した試験区3の醤油ラーメンスープは、塩味が最も強く、かつ風味もよいものであった。 As shown in Table 1, powder 1 obtained by subjecting soy sauce ramen soup (salt-reduced type) to amino-carbonyl reaction of separated defatted soybean protein hydrolyzate and n-hexanal and succinic acid were added. The soy sauce ramen soup had the strongest saltiness and good flavor.
(実施例2)
分離脱脂大豆蛋白質加水分解物のかわりにゼラチンを蛋白質分解酵素で加水分解して得られたゼラチン加水分解物(協和発酵フーズ社製)を用いる以外は実施例1と同様の操作を行って粉末2を得た。
(Example 2)
Powder 2 was prepared in the same manner as in Example 1 except that gelatin hydrolyzate obtained by hydrolyzing gelatin with proteolytic enzyme (manufactured by Kyowa Hakko Foods) was used in place of the separated defatted soybean protein hydrolyzate. Got.
実施例1で調製した醤油ラーメンスープ(減塩タイプ)100mlに、粉末2およびコハク酸を、第2表に示すように、単独で、または組み合わせて添加した。 As shown in Table 2, powder 2 and succinic acid were added alone or in combination to 100 ml of soy sauce ramen soup (salt-reduced type) prepared in Example 1.
これらの醤油ラーメンスープの中で感じられる塩味、醤油の風味および醤油ラーメンスープとしての好ましさについて、7名のトレーニングされたパネラーにより官能評価を行った。 The sensory evaluation was carried out by seven trained panelists regarding the salty taste, soy sauce flavor, and the preference for soy sauce ramen soup.
評価は、各評価項目において、評価の最も低いものを1点、評価の最も高いものを7点とし、醤油ラーメンスープ(減塩タイプ)を3点とする7点評価法で行った。 In each evaluation item, the evaluation was performed by a 7-point evaluation method in which the lowest evaluation was 1 point, the highest evaluation was 7 points, and soy sauce ramen soup (salted salt type) was 3 points.
結果を第2表に示す。
第2表に示すとおり、醤油ラーメンスープ(減塩タイプ)に、ゼラチン加水分解物とn−ヘキサナールとをアミノ−カルボニル反応させて得た粉末2およびコハク酸を添加した試験区3の醤油ラーメンスープは、食塩味が最も強く、かつ風味もよいものであった。 As shown in Table 2, the soy sauce ramen soup in Test Zone 3 was prepared by adding powder 2 obtained by subjecting a soy sauce ramen soup (reduced salt type) to an amino-carbonyl reaction of gelatin hydrolyzate and n-hexanal and succinic acid. Had the strongest salty taste and good flavor.
(実施例3)
5gの分離脱脂大豆蛋白質加水分解物のかわりに4gの酵母エキス(協和発酵フーズ社製)を用い、0.12gのn−ヘキサナールのかわりに1gのグルコースを用いる以外は実施例1と同様の操作を行って粉末3を得た。
(Example 3)
The same operation as in Example 1 except that 4 g of yeast extract (manufactured by Kyowa Hakko Foods Co., Ltd.) was used instead of 5 g of separated defatted soybean protein hydrolyzate, and 1 g of glucose was used instead of 0.12 g of n-hexanal. To obtain powder 3.
また、濃い口醤油、だし汁、砂糖、醸造調味料、カツオエキス、昆布エキス、水等を用いて、常法により塩化ナトリウム濃度1.23重量%のめんつゆ(通常タイプ)、および塩化ナトリウム濃度を0.93重量%としためんつゆ(減塩タイプ)を調製した。 Also, using a thick mouth soy sauce, soup stock, sugar, brewed seasoning, bonito extract, kelp extract, water, etc., noodle soup (normal type) with a sodium chloride concentration of 1.23% by weight and a sodium chloride concentration of 0. A soup sauce (reduced salt type) was prepared at 93% by weight.
めんつゆ(減塩タイプ)100mlに、粉末3およびコハク酸を、第3表に示すように、単独で、または組み合わせて添加した。 As shown in Table 3, Powder 3 and succinic acid were added alone or in combination to 100 ml of mentsuyu (reduced salt type).
これらのめんつゆの中で感じられる食塩味、醤油の風味およびめんつゆとしての好ましさについて、7名のトレーニングされたパネラーにより官能評価を行った。 Sensory evaluation was performed by 7 trained panelists regarding the salty taste, soy sauce flavor, and the preference for noodle soup that can be felt in these noodle soups.
評価は、各評価項目において、評価の最も低いものを1点、評価の最も高いものを7点とし、めんつゆ(減塩タイプ)を3点とする7点評価法で行った。 In each evaluation item, the evaluation was performed by a 7-point evaluation method in which the lowest evaluation was 1 point, the highest evaluation was 7 points, and noodle soup (salt reduction type) was 3 points.
結果を第3表に示す。
第3表に示すとおり、めんつゆ(減塩タイプ)に、酵母エキスとグルコースとをアミノ−カルボニル反応させて得た粉末3およびコハク酸を添加した試験区3のめんつゆは、塩味が最も強く、かつ風味もよいものであった。 As shown in Table 3, the powder 3 obtained by subjecting the yeast extract and glucose to amino-carbonyl reaction to the noodle soup (salt-reducing type) and the noodle soup in the test section 3 to which succinic acid was added had the strongest salty taste, and The flavor was also good.
(実施例4)
分離脱脂大豆蛋白質200gを水1800mlに分散させ、アルカラーゼ(ノボザイムズ社製)を4ml加えて、50℃で30分間反応させた。さらに水酸化ナトリウムでpH8に維持しながら50℃で20時間反応させた。
Example 4
200 g of separated defatted soybean protein was dispersed in 1800 ml of water, 4 ml of Alcalase (manufactured by Novozymes) was added, and the mixture was reacted at 50 ° C. for 30 minutes. The mixture was further reacted at 50 ° C. for 20 hours while maintaining the pH at 8 with sodium hydroxide.
反応終了後、85〜90℃で20分間加熱して酵素を失活させた後、遠心分離した。得られた上清をろ過し、ろ液を限外ろ過(分画分子量1000〜5000の限外ろ過膜を使用)に供し、分子量1000〜5000の画分を得た。得られた画分を凍結乾燥させて粉末を得た。 After completion of the reaction, the enzyme was inactivated by heating at 85 to 90 ° C. for 20 minutes, and then centrifuged. The obtained supernatant was filtered, and the filtrate was subjected to ultrafiltration (using an ultrafiltration membrane having a molecular weight cut off of 1000 to 5000) to obtain a fraction having a molecular weight of 1000 to 5000. The obtained fraction was freeze-dried to obtain a powder.
該粉末4gを、0.8gのキシロースを含む水溶液100mlに溶解し、95℃で4時間反応させた。 4 g of the powder was dissolved in 100 ml of an aqueous solution containing 0.8 g of xylose and reacted at 95 ° C. for 4 hours.
得られた反応液を限外ろ過(分画分子量1000の限外ろ過膜を使用)に供し、分子量1000以上の画分を得た。該画分を凍結乾燥して約2gの粉末4を得た。 The obtained reaction solution was subjected to ultrafiltration (using an ultrafiltration membrane having a fractional molecular weight of 1000) to obtain a fraction having a molecular weight of 1,000 or more. The fraction was freeze-dried to obtain about 2 g of powder 4.
また、0.9重量%の塩化ナトリウム水溶液を調製し、該水溶液に粉末4およびコハク酸を、第4表に示すように、それぞれ単独で、または組み合わせて添加した。 In addition, a 0.9 wt% sodium chloride aqueous solution was prepared, and powder 4 and succinic acid were added to the aqueous solution alone or in combination as shown in Table 4.
得られた各溶液の塩味について、7名のトレーニングされたパネラーにより官能評価を行った。 About the salty taste of each obtained solution, sensory evaluation was performed by seven trained panelists.
評価は、以下の指標に従って7点評価で行い、各パネラーの評価の平均値を求めた。 Evaluation was performed by 7-point evaluation according to the following indices, and an average value of evaluation of each panel was obtained.
結果を第4表に示す。 The results are shown in Table 4.
7点:非常に強く塩味を感じる
6点:塩味を強く感じる
5点:塩味を少し強く感じる
4点:塩味を感じる
3点:少し塩味を感じる
2点:わずかに塩味を感じる
1点:まったく塩味を感じない
6 points: feels a strong saltiness 5 points: feels a little salty 4 points: feels a salty 3 points: feels a little salty 2 points: feels a little salty 1 point: does not feel a salty taste at all
第4表に示すとおり、分離脱脂大豆蛋白質由来の分子量1000〜5000のペプチドとキシロースとをアミノ−カルボニル反応させて得た粉末4およびコハク酸を添加した試験区3では強めの塩味が感じられ、かつコハク酸による異風味も感じられなかった。 As shown in Table 4, the powder 4 obtained by amino-carbonyl reaction of a peptide having a molecular weight of 1000 to 5000 derived from separated defatted soy protein and xylose and the test group 3 to which succinic acid was added had a strong salty taste. In addition, an unusual flavor due to succinic acid was not felt.
(実施例5)
実施例1で調製した醤油ラーメンスープ(減塩タイプ)100mlに、実施例4で得た粉末4およびコハク酸を、第5表に示すように、単独で、または組み合わせて添加した。
(Example 5)
As shown in Table 5, the powder 4 obtained in Example 4 and succinic acid were added alone or in combination to 100 ml of soy sauce ramen soup prepared in Example 1 (reduced salt type).
これらの醤油ラーメンスープの中で感じられる塩味、醤油特有の風味および醤油ラーメンスープとしての好ましさについて、7名のトレーニングされたパネラーにより官能評価を行った。 The sensory evaluation was performed by 7 trained panelists about the salty taste, the unique flavor of soy sauce, and the preference of soy sauce ramen soup.
評価は、各評価項目において、評価の最も低いものを1点、評価の最も高いものを7点とし、醤油ラーメンスープ(減塩タイプ)を3点とする7点評価法で行った。 In each evaluation item, the evaluation was performed by a 7-point evaluation method in which the lowest evaluation was 1 point, the highest evaluation was 7 points, and soy sauce ramen soup (salted salt type) was 3 points.
結果を第5表に示す。
第5表に示すとおり、減塩タイプの醤油ラーメンスープに、分離脱脂大豆蛋白質由来の分子量1000〜5000のペプチドとキシロースとをアミノ−カルボニル反応させて得た粉末4およびコハク酸を添加した試験区3の醤油ラーメンスープは、塩味が最も強く、かつ風味もよいものであった。 As shown in Table 5, powder 4 obtained by subjecting a salt-reduced soy sauce ramen soup to an amino-carbonyl reaction between a peptide having a molecular weight of 1000 to 5000 derived from separated defatted soybean protein and xylose and succinic acid were added. No. 3 soy sauce ramen soup had the strongest salty taste and good flavor.
(実施例6)
実施例3で調製しためんつゆ(減塩タイプ)100mlに、実施例4で得た粉末4およびコハク酸を、第6表に示すように、単独で、または組み合わせて添加した。
(Example 6)
As shown in Table 6, the powder 4 obtained in Example 4 and succinic acid were added alone or in combination to 100 ml of soy sauce prepared in Example 3 (reduced salt type).
これらのめんつゆの中で感じられる塩味、醤油特有の風味およびめんつゆとしての好ましさについて、7名のトレーニングされたパネラーにより官能評価を行った。 The sensory evaluation was performed by seven trained panelists regarding the salty taste, soy sauce-specific flavor, and preference for noodle soup that can be felt in these noodle soups.
評価は、各評価項目において、評価の最も低いものを1点、評価の最も高いものを7点とし、めんつゆ(減塩タイプ)を3点とする7点評価法で行った。 In each evaluation item, the evaluation was performed by a 7-point evaluation method in which the lowest evaluation was 1 point, the highest evaluation was 7 points, and noodle soup (salt reduction type) was 3 points.
結果を第6表に示す。
第6表に示すとおり、減塩タイプのめんつゆに、分離脱脂大豆蛋白質由来の分子量1000〜5000のペプチドとキシロースとをアミノ−カルボニル反応させて得た粉末4およびコハク酸を添加した試験区3のめんつゆは、塩味が最も強く、かつ風味もよいものであった。 As shown in Table 6, powder 4 obtained by amino-carbonyl reaction of a peptide having a molecular weight of 1000 to 5000 derived from separated defatted soy protein and xylose to salt-reduced noodle soup and succinic acid were added. Mentsuyu had the strongest salty taste and good flavor.
(実施例7)
加工用赤味噌および水を用いて、常法により塩化ナトリウム濃度1.46重量%の味噌汁(通常タイプ)、および塩化ナトリウム濃度を1.18重量%とした味噌汁(減塩タイプ)を調製した。
(Example 7)
Miso soup with a sodium chloride concentration of 1.46% by weight (normal type) and miso soup with a sodium chloride concentration of 1.18% by weight (reduced salt type) were prepared by a conventional method using red miso for processing and water.
味噌汁(減塩タイプ)100mlに、実施例4で得た粉末4およびコハク酸を、第7表に示すように、単独で、または組み合わせて添加した。 As shown in Table 7, the powder 4 obtained in Example 4 and succinic acid were added singly or in combination to 100 ml of miso soup (salt-reduced type).
これらの味噌汁の中で感じられる食塩味、味噌の風味および味噌汁としての好ましさについて、7名のトレーニングされたパネラーにより官能評価を行った。 The sensory evaluation was conducted by 7 trained panelists about the salty taste, miso flavor and taste preference of these miso soups.
評価は、各評価項目において、評価の最も低いものを1点、評価の最も高いものを7点とし、味噌汁(減塩タイプ)を3点とする7点評価法で行った。 In each evaluation item, the evaluation was performed by a 7-point evaluation method in which the lowest evaluation was 1 point, the highest evaluation was 7 points, and miso soup (salt-reducing type) was 3 points.
結果を第7表に示す。
第7表に示すとおり、減塩味噌汁に、分離大豆蛋白質由来の分子量1000〜5000のペプチドとキシロースとをアミノ−カルボニル反応させて得た粉末4およびコハク酸を添加した試験区3の味噌汁は、塩味が最も強く、かつ風味もよいものであった。 As shown in Table 7, the powder 4 obtained by amino-carbonyl reaction of a peptide having a molecular weight of 1000 to 5000 derived from isolated soy protein and xylose to the reduced salt miso soup, and the miso soup of test section 3 to which succinic acid was added, It had the strongest salty taste and good flavor.
(実施例8)
実施例4で得た分子量1000〜5000の画分の粉末4gを、0.8gのガラクツロン酸を含む水溶液100mlに溶解し、95℃で4時間反応させた。得られた反応液を限外ろ過(分画分子量1000の限外ろ過膜を使用)に供し、分子量1000以上の画分を得た。該画分を凍結乾燥して約2gの粉末5を得た。
(Example 8)
4 g of the powder having a molecular weight of 1000 to 5000 obtained in Example 4 was dissolved in 100 ml of an aqueous solution containing 0.8 g of galacturonic acid and reacted at 95 ° C. for 4 hours. The obtained reaction solution was subjected to ultrafiltration (using an ultrafiltration membrane having a fractional molecular weight of 1000) to obtain a fraction having a molecular weight of 1,000 or more. The fraction was freeze-dried to obtain about 2 g of powder 5.
また、ホワイトルゥ、バター、粉末油脂、脱脂粉乳、コーンスターチ、チキンブイヨン、野菜ブイヨン等を用いて、塩化ナトリウム濃度0.88重量%とする以外は、常法によりクリームスープ(減塩タイプ)を調製した。 Also, cream soup (salt-reduced type) is prepared in a conventional manner except that white sodium chloride, butter, powdered oil and fat, nonfat dry milk, corn starch, chicken bouillon, vegetable bouillon, etc. is used with a sodium chloride concentration of 0.88% by weight. did.
該クリームスープ100mlに、粉末5およびコハク酸を、第8表に示すように、単独で、または組み合わせて添加した。 To 100 ml of the cream soup, powder 5 and succinic acid were added alone or in combination as shown in Table 8.
得られたクリームスープの中で感じられる食塩味、乳製品特有のクリーム風味およびクリームスープとしての好ましさについて、7名のトレーニングされたパネラーにより官能評価を行った。 Sensory evaluation was performed by seven trained panelists on the salty taste, the cream flavor peculiar to dairy products, and the preference of the cream soup as felt in the obtained cream soup.
評価は、各評価項目において、評価の最も低いものを1点、評価の最も高いものを7点とし、クリームスープ(減塩タイプ)を3点とする7点評価法で行った。 For each evaluation item, the evaluation was performed by a 7-point evaluation method in which the lowest evaluation was 1 point, the highest evaluation was 7 points, and the cream soup (salt reduction type) was 3 points.
結果を第8表に示す。
第8表に示すとおり、クリームスープ(減塩タイプ)に、分離大豆蛋白質由来の分子量1000〜5000のペプチドとガラクツロン酸とをアミノ−カルボニル反応させて得た粉末5およびコハク酸を添加した試験区3のクリームスープは、塩味が最も強く、かつ風味もよいものであった。 As shown in Table 8, powder 5 obtained by subjecting cream soup (salt-reduced type) to amino-carbonyl reaction of a peptide having a molecular weight of 1000 to 5000 derived from isolated soy protein and galacturonic acid and succinic acid were added. The cream soup of 3 had the strongest salty taste and good flavor.
(実施例9)
実施例1で調製した醤油ラーメンスープ(減塩タイプ)100mlに、実施例8で得た粉末5およびリンゴ酸を、第9表に示すように、単独で、または組み合わせて添加した。
Example 9
As shown in Table 9, the powder 5 and malic acid obtained in Example 8 were added alone or in combination to 100 ml of soy sauce ramen soup prepared in Example 1 (reduced salt type).
これらの醤油ラーメンスープの中で感じられる食塩味、醤油の風味および醤油ラーメンスープとしての好ましさについて、7名のトレーニングされたパネラーにより官能評価を行った。 Sensory evaluation was performed by 7 trained panelists regarding the salty taste, soy sauce flavor, and the preference for soy sauce ramen soup.
評価は、各評価項目において、評価の最も低いものを1点、評価の最も高いものを7点とし、醤油ラーメンスープ(減塩タイプ)を3点とする7点評価法で行った。 In each evaluation item, the evaluation was performed by a 7-point evaluation method in which the lowest evaluation was 1 point, the highest evaluation was 7 points, and soy sauce ramen soup (salted salt type) was 3 points.
結果を第9表に示す。
第9表に示すとおり、醤油ラーメンスープ(減塩タイプ)に、分離脱脂大豆蛋白質由来の分子量1000〜5000のペプチドとガラクツロン酸とをアミノ−カルボニル反応させて得た粉末5およびリンゴ酸を添加した試験区3の醤油ラーメンスープは、食塩味が強く、かつ風味もよいものであった。 As shown in Table 9, powder 5 and malic acid obtained by amino-carbonyl reaction of a peptide having a molecular weight of 1000 to 5000 derived from separated defatted soybean protein and galacturonic acid were added to soy sauce ramen soup (low salt type). The soy sauce ramen soup in Test Zone 3 had a strong salty taste and a good flavor.
(実施例10)
実施例4で得た分離脱脂大豆蛋白質由来の分子量1000〜5000の画分の粉末4gを、0.8gのデキストリン(パインデックス#4:松谷化学社)を含む水溶液100mlに溶解し、95℃で4時間反応させた。得られた反応液を限外ろ過(分画分子量1000の限外ろ過膜を使用)に供して分子量1000以上の画分を約2gのアミノ−カルボニル反応物6として得た。
(Example 10)
4 g of the powder having a molecular weight of 1000 to 5000 derived from the separated defatted soybean protein obtained in Example 4 was dissolved in 100 ml of an aqueous solution containing 0.8 g of dextrin (Paindex # 4: Matsutani Chemical Co., Ltd.) at 95 ° C. The reaction was performed for 4 hours. The obtained reaction solution was subjected to ultrafiltration (using an ultrafiltration membrane having a molecular weight cut off of 1000) to obtain a fraction having a molecular weight of 1000 or more as about 2 g of amino-carbonyl reactant 6.
実施例3で調製しためんつゆ(減塩タイプ)100mlに、上記のように調製したアミノ−カルボニル反応物およびリンゴ酸を、第10表に示すように、単独で、または組み合わせて添加した。 As shown in Table 10, the amino-carbonyl reactant and malic acid prepared as described above were added to 100 ml of the soup soup prepared in Example 3 (reduced salt type) alone or in combination.
これらのめんつゆの中で感じられる食塩味、醤油の風味およびめんつゆとしての好ましさについて、4名のトレーニングされたパネラーにより官能評価を行った。 Sensory evaluation was performed by four trained panelists regarding the salty taste, soy sauce flavor and the preference for noodle soup that can be felt in these noodle soups.
評価は、各評価項目において、評価の最も低いものを1点、評価の最も高いものを7点とし、めんつゆ(減塩タイプ)を3点とする7点評価法で行った。 In each evaluation item, the evaluation was performed by a 7-point evaluation method in which the lowest evaluation was 1 point, the highest evaluation was 7 points, and noodle soup (salt reduction type) was 3 points.
結果を第10表に示す。
第10表に示すとおり、分離脱脂大豆蛋白質由来の分子量1000〜5000のペプチドとデキストリンとをアミノ−カルボニル反応させて得られたアミノ−カルボニル反応物6およびリンゴ酸を添加した試験区3のめんつゆは食塩味が強く、かつめんつゆとしての好ましさもより強いものであった。 As shown in Table 10, the amino-carbonyl reactant 6 obtained by subjecting a peptide having a molecular weight of 1000 to 5000 derived from separated defatted soybean protein and dextrin to amino-carbonyl reaction and malt acid in test group 3 were added. The salty taste was strong and the preference for bonito soup was stronger.
本発明は、食塩の含有量を低減した減塩飲食品の製造・加工分野において利用できる。 INDUSTRIAL APPLICABILITY The present invention can be used in the field of manufacturing and processing reduced-salt foods and drinks with a reduced salt content.
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