JP5369255B2 - Magnetic separator, magnetic separator, and magnetic separation method - Google Patents

Description

  The present invention relates to a magnetic separator, a magnetic separator, and a magnetic separation method for magnetically separating a magnetic substance from a fluid.

Cell and microorganism culture fluids, other extracts, plating wastewater, fluids in which heavy metals and rare metals are dissolved, other wastewater, groundwater, and other substances that are dissolved using magnetic beads or magnets with magnetism. The substance to be absorbed is absorbed and concentrated using a magnetic separation device.
A superconducting magnet that generates a strong magnetic field is used in a magnetic separation device that separates magnetic substances (magnetic beads and magnetic bodies) adsorbing these dissolved substances from the raw fluid.

  For example, the superconducting magnetic separation type fluid processing apparatus disclosed in Patent Document 1 is a superconducting magnetic separation type that magnetically separates a substance to be separated from a fluid containing the substance to be separated provided with magnetism in a bore of a superconducting coil. A fluid treatment device comprising an umbrella-shaped magnetic filter composed of a plurality of magnetic wires extending radially from the center in a bore of a superconducting coil, and a substance to be separated guided and captured by the magnetic wire in the center of the magnetic filter. It is comprised so that it may attract from a part or a peripheral part (Claim 5 of patent documents 1).

  Patent Document 1 describes that the superconducting coil has a maximum magnetic force at the center of the magnetic field in the center of the bore axis when the magnetic field in the bore is calculated.

JP 2009-106854 A

In the prior art, a fluid containing a material to be separated is flowed in one direction, and the material to be separated is separated and captured and collected by the magnetic force of a filter member (magnetic filter) made of a magnetic wire. Since the substance to be separated captured by the filter member may flow by the fluid, and the magnetic substance may block at the center of the magnetic field and the fluid may not flow. It is necessary to remove the magnetic substance from the conductive magnet and clean it.

It is an object of the present invention to provide a magnetic separation tool, a magnetic separation device, and a magnetic separation method that can solve such problems of the prior art.
In the present invention, the magnetic material is attracted from the raw fluid flowing into the cylinder to the center of the magnetic field by the magnetic separation unit by the magnetic separation, and the purified fluid after magnetic separation of the magnetic material is attracted by the inflow direction of the raw fluid and the magnetic material by the magnetic force. It is an object of the present invention to provide a magnetic separation tool, a magnetic separation device, and a magnetic separation method that can separate and accumulate magnetic materials more reliably by flowing in a direction opposite to the direction in which the magnetic material is flown.

Specific means for solving the problems in the present invention are as follows.
First, the magnetic separator is an inflow path through which the raw fluid S1 flows from the outside of the bore 2a toward the magnetic field center G side into the cylinder inserted from the outside of the superconducting magnet 2 toward the magnetic field center G side in the bore 2a. 12a, a magnetic separation unit M that separates the magnetic substance P from the raw fluid S1 by a magnetic force, an accumulation unit Q that accumulates the magnetic substance P separated by the magnetic separation unit M near the magnetic field center G, and the magnetic separation unit M And an outflow passage 12b through which the purified fluid S2 flows in the direction opposite to the inflow direction of the raw fluid S1 and the direction in which the magnetic substance P is attracted by the magnetic force and is discharged out of the bore 2a ,
The cylindrical body has an inner / outer double cylinder structure, and is inserted into the outer cylinder 3 and a bottomed cylindrical outer cylinder 3 that forms the magnetic separation part M and the accumulation part Q on the outer cylinder bottom wall 3a side. And the inner cylinder 4 forming the inflow path 12a, and the outflow path 12b is formed between the inner peripheral surface of the outer cylinder 3 and the outer peripheral surface of the inner cylinder 4 .

Secondly, the magnetic separator is an inflow path through which the raw fluid S1 flows from the outside of the bore 2a toward the magnetic field center G side into the cylinder inserted from the outside of the superconducting magnet 2 toward the magnetic field center G side in the bore 2a. 12a, a magnetic separation unit M that separates the magnetic substance P from the raw fluid S1 by a magnetic force, an accumulation unit Q that accumulates the magnetic substance P separated by the magnetic separation unit M near the magnetic field center G, and the magnetic separation unit M And an outflow passage 12b through which the purified fluid S2 flows in the direction opposite to the inflow direction of the raw fluid S1 and the direction in which the magnetic substance P is attracted by the magnetic force and is discharged out of the bore 2a,
The cylindrical body is magnetically separated by the magnetic separation unit M and the magnetic substance P to be stacked in the stacking unit Q in the intake magnetic separation device and the third, the cylindrical body is an integrated part Q conical concave bottom wall 3a And an extraction tube 5 that is magnetically separated in the magnetic separation part M and is collected in the accumulation part Q is connected to the center side of the bottom wall 3a. .

First, the magnetic separator is characterized in that the magnetic separator 1 is inserted into the bore 2a of the superconducting magnet 2 and is detachably mounted .
Secondly, the magnetic separation device inserts the magnetic separator 1 into the magnetic field center G side from one end of the bore 2a of the superconducting magnet 2 and attaches it detachably, and attaches the extraction tube 5 to the other end side of the bore 2a. It is characterized by being extended .

Magnetic separation device 3, the magnetic separation device 1, characterized in that it is arranged by inserting a pair of opposite form from both ends of the bore 2a of the superconducting magnet 2 to the magnetic field center G side.
In the magnetic separation method, first, the raw fluid S1 is caused to flow from the outside of the bore 2a toward the magnetic field center G side into the cylinder inserted from the outside of the superconducting magnet 2 to the magnetic field center G side in the bore 2a. The magnetic substance P is separated from the fluid S1 by a magnetic force and accumulated in the accumulation portion Q near the magnetic field center G, and the purified fluid S2 separated from the magnetic substance P is introduced into the original fluid S1 and the magnetic substance P is attracted by the magnetic force. And flowing out of the bore 2a , and the magnetic substance P accumulated by the magnetic force in the accumulating portion Q in the cylinder is sucked out by the extraction tube 5 and extracted .

Secondly, the magnetic separation method allows the raw fluid S1 to flow from the outside of the bore 2a toward the magnetic field center G side into the cylinder inserted from the outside of the superconducting magnet 2 to the magnetic field center G side in the bore 2a. The magnetic substance P is separated from the fluid S1 by a magnetic force and accumulated in the accumulation portion Q near the magnetic field center G, and the purified fluid S2 separated from the magnetic substance P is introduced into the original fluid S1 and the magnetic substance P is attracted by the magnetic force. And flow out of the bore 2a,
The raw fluid S1 and the purified fluid S2 that flows in the opposite direction to the raw fluid S1 are separated from the raw fluid S1.
It is made to flow by the water head difference of the inlet 4a and the discharge port 3b of the purification fluid S2 .

Third, the magnetic separation method collects the magnetic substance P from the outer peripheral part of the cylindrical body to the central side by making the accumulation part Q conical concave, and the magnetic substance P flows from the central side into the raw fluid S1 through the extraction pipe 5. It is taken out in the direction .

According to the present invention, after separating the magnetic substance from the raw fluid that has flowed into the cylindrical body on the magnetic field center side, the purified fluid flows in the direction opposite to the inflow direction of the original fluid and the direction in which the magnetic substance is attracted by the magnetic force. The magnetic substance can be more reliably separated, and the separated magnetic substances can be reliably collected.
That is, the magnetic separator firstly forms an inflow passage 12a, a magnetic separation portion M, an accumulation portion Q, and an outflow passage 12b in the cylinder, and the inflow direction of the raw fluid S1 and the magnetic substance P from the magnetic separation portion M. Since the purified fluid S2 is caused to flow in a direction opposite to the direction attracted by the magnetic force, the flow of the magnetic material P by the purified fluid S2 can be reduced, and the magnetic material P can be more reliably separated, and the magnetically separated magnetic material P is separated. Can be reliably accumulated in the accumulating portion Q that is not affected by the flow of the purification fluid S2.

Secondly, the magnetic separator has an inner / outer double cylinder structure, so that the magnetic separation part M and the accumulation part Q are arranged on the outer cylinder bottom wall 3 a side of the outer cylinder 3, and the outer cylinder 3 and the inner cylinder 4. The outflow passage 12b can be easily and easily formed between the two.
Thirdly, the magnetic separator is provided with the extraction tube 5 in the cylindrical body, so that the magnetic substance P that is magnetically separated in the magnetic separation part M and accumulated in the accumulation part Q near the magnetic field center G can be easily and reliably obtained. Suction can be taken out, and the raw fluid S1 can be continuously flowed in for continuous magnetic separation.

Fourth, the magnetic separator is provided with the high gradient magnetic capture member 25 in the cylindrical body, so that the magnetic substance P can be captured from the raw fluid S1, and the magnetic substance P is densely separated from the high gradient magnetic capture member 25. Even so, it can be accumulated on the magnetic field center G side in the flow direction of the raw fluid S1.
Fifthly, in the magnetic separator, when the cross-sectional area of the inflow passage 12a and the cross-sectional area of the outflow passage 12b are set to be substantially the same, the flow rates of the raw fluid S1 and the purified fluid S2 are substantially the same, and the fluid S is directional. Only by changing the flow, the flow is caused by the difference in water head formed by the inlet 4a and the outlet 3b, and the magnetic substance P is hardly affected by the flow of the fluid S in the magnetic separation part M and the accumulation part Q.

The magnetic separator can be mounted by inserting the magnetic separator 1 into the bore 2a of the superconducting magnet 2 after the magnetic separator 1 is assembled.
In the magnetic separation method, first, the magnetic material P is magnetically separated by flowing the raw fluid S1 to the magnetic separation unit M, and the magnetic material P that has been magnetically separated is reliably accumulated in the accumulation unit Q near the magnetic field center G. Since the purifying fluid S2 flows in the direction opposite to the inflow direction of the original fluid S1 from the magnetic separation unit M and the direction in which the magnetic substance P is attracted by the magnetic force, the magnetic substance P accumulated in the accumulating part Q is influenced by the flow of the purifying fluid S2. Therefore, the flow of the magnetic substance P by the purification fluid S2 can be reduced, and the magnetic substance P can be more reliably separated and accumulated.

Secondly, the magnetic separation method allows the magnetic substance P, which is magnetically separated in the magnetic separation unit M and accumulated in the accumulation unit Q near the magnetic field center G, to be easily and reliably attracted and extracted by the extraction tube 5. The magnetic separation can be continuously performed by continuously flowing the raw fluid S1.
Third, the magnetic separation method can capture the magnetic material P by the high gradient magnetic capture member 25 from the raw fluid S1 before reaching the magnetic field center G side, and the magnetic material P is densely separated from the high gradient magnetic capture member 25. Even so, it can be accumulated on the magnetic field center G side in the flow direction of the raw fluid S1.

  Thirdly, in the magnetic separation method, when the raw fluid S1 and the purified fluid S2 flowing in the opposite direction of the raw fluid S1 are caused to flow due to a water head difference between the inlet 4a of the raw fluid S1 and the outlet 3b of the purified fluid S2. The fluid S hardly changes the flow of the fluid S to the magnetic substance P in the magnetic separation part M and the accumulation part Q only by changing the direction.

It is a section front view showing a 1st embodiment of the present invention. It is the XX sectional view taken on the line of FIG. It is a section front view showing a 2nd embodiment. It is a side view which shows 2nd Embodiment. It is a section front view showing a 3rd embodiment. FIG. 6 is a cross-sectional view taken along line YY in FIG. 5. It is a section front view showing a 4th embodiment. It is a side view which shows 5th Embodiment. It is a section front view showing a 6th embodiment. It is a section front view showing a 7th embodiment. It is an isomagnetic field diagram which shows magnetic strength distribution of the magnetic field in the bore | bore of a superconducting magnet.

Hereinafter, embodiments of the present invention will be described with reference to the drawings.
In the first embodiment shown in FIGS. 1 and 2, the magnetic separation device 11 includes a superconducting magnet 2 and a magnetic separator 1 </ b> A inserted into the superconducting magnet 2, and cultures cells and microorganisms. Liquids, other extracts, plating wastewater, fluids in which heavy metals and rare metals are dissolved, other wastewater, materials dissolved in groundwater, etc., using magnetic substances (magnetic beads and magnetic bodies with magnetism) P The magnetic substance P is separated from the fluid by using the magnetic force of the superconducting magnet 2 and accumulated, and continuously collected in a concentrated state.

The superconducting magnet 2 has a bore 2a formed in a superconducting coil, and is arranged vertically so that the axis of the bore 2a is vertical.
The magnetic force in the magnetic field of the superconducting magnet 2 is different in each part in the bore 2a. As shown in FIG. 11, the part F1 in the center of the bore 2a in the axial center direction and near the inner peripheral surface of the bore 2a is the highest. The part F2 that is separated from the part F1 in the axial direction of the bore 2a and reaches the axis from the inner peripheral surface of the bore 2a is high, and the part F3 that is separated from the part F2 outward in the axial direction of the bore 2a is high. The magnetic force decreases as the distance from the portion F3 increases outward in the axial direction of the bore 2a.

For example, in the superconducting magnet 2 with the bore 2a having an inner diameter of 30 cm, when the highest magnetic field part F1 is 24,000 gauss, the high magnetic field part F2 is 20,000 gauss and 10,000 gauss per part F12.
As described above, the magnetic field of the superconducting magnet 2 has the maximum magnetic field at the magnetic field center G and the largest magnetic force, and the magnetic separator 1A is inserted from the outside of the bore 2a to the vicinity of the magnetic field center G.

  The magnetic separator 1A is a cylindrical body having an inner and outer double structure, and has a bottomed cylinder having a discharge port 3b for fluid (purified fluid, waste liquid) S2 on the outer end side and an outer cylinder bottom wall 3a on the inner end side. An outer cylinder 3 having a shape, and an inner cylinder 4 having an intake 4a inserted into the outer cylinder 3 and taking in a raw fluid (raw solution) S1 containing a magnetic substance P on the outer end side, The inner tube 4 has an extraction tube 5 that is inserted inward from the outer end side, and whose tip protrudes from the inner end of the inner tube 4 to the outer tube bottom wall 3a side.

  The outer cylinder 3, the inner cylinder 4 and the extraction pipe 5 are substantially concentric triple pipes, which are arranged substantially concentrically with the axis C of the bore 2a. The outer tube 3, the inner tube 4 and the extraction tube 5 are made of a nonmagnetic material such as metal, resin or glass, and can be made of, for example, stainless steel, polypropylene (PP), transparent acrylic resin, etc. Application is preferred from the viewpoint of adsorption prevention.

The outer cylinder 3 is formed in a bottomed cylindrical shape whose outer peripheral surface is close to or in close contact with the inner surface of the bore 2a, forms a casing, and an inner end inserted into the bore 2a is an outer cylinder bottom wall 3a. The outer end is closed by a closing member 15 provided between the inner cylinder 4 and a discharge port 3b is formed on the outer peripheral surface near the outer end, and a discharge pipe 16 is connected to the discharge port 3b. Yes.
The outer cylinder bottom wall 3a of the outer cylinder 3 is inserted into the bore 2a and disposed in the vicinity of the magnetic field center G and beyond the magnetic field center G, and the upper side of the outer cylinder bottom wall 3a serves as a pool for the magnetic substance P. The accumulating portion Q is a portion where the raw fluid S1 coming out from the inner end of the inner cylinder 4 changes the flow in the opposite direction as the purified fluid S2, in which the magnetic substance P is a magnetic field. The magnetic separation part M is separated by being pulled by a magnetic force in the center G direction.

The outer cylinder 3 is placed in a vertically inserted state in the bore 2a of the superconducting magnet 2 via a mounting tool 6 and is detachably attached to the superconducting magnet 2. The mounting tool 6 includes an annular mounting plate 6a that is fixed to the outer periphery of an intermediate portion in the axial direction of the outer cylinder 3, a gap member 6b that is provided on the outer periphery of the inner end of the outer cylinder 3, and a magnetic separation from the annular mounting plate 6a. A spacer 22 is interposed between the tool 1A and the outer cylinder bottom wall 3a (inner end side) of the outer cylinder 3 is inserted into the bore 2a, and the gap 2b is adjusted with the gap member 6b. The upper surface of the annular mounting plate 6a and the magnetic separator 1A is fixed with a fastener such as a bolt.

The inner cylinder 4 is formed in a cylindrical shape having openings at both ends, the opening at the inner end inserted into the bore 2a is disposed to face the outer cylinder bottom wall 3a at a distance in the axial direction, and the outer end is an extraction pipe. The intake port 4a is formed on the outer peripheral surface in the vicinity of the outer end, and the inflow pipe 18 is connected to the intake port 4a.
The inner cylinder 4 is attached in the outer cylinder 3 by a support 7 provided between the inner cylinder 4 and the outer cylinder 3. The support 7 includes the closing member 15 fixed to the outer periphery of the middle portion of the inner cylinder 4 in the axial direction, the outer cylinder flange 6c fixed to the outer peripheral surface of the outer cylinder 3, and the closing member 15 and the outer cylinder. And a spacer 23 interposed between the flange 6c, the closing member 15 is detachably fixed to the outer cylinder flange 6c from above, and the position of the inner cylinder 4 is fixed with respect to the outer cylinder 3. Close the top of the gap between them.

  The inner cylinder 4 forms an inflow path 12a through which the raw fluid S1 introduced from the inflow pipe 18 through the inlet 4a flows toward the magnetic separation portion M of the outer cylinder 3, and the inner cylinder 4 and the outer cylinder 3 Are formed with an annular outflow passage 12b through which the purified fluid S2 after separating the magnetic substance P from the magnetic separation portion M is discharged from the discharge pipe 16 through the discharge port 3b. The fluid S containing the magnetic substance P flows into the superconducting magnet 2 from the outside, and then the flow path 12 is formed which leads from the inside of the superconducting magnet 2 to the outside again.

  That is, in the cylindrical body constituting the magnetic separator 1, the inflow path 12 a for allowing the raw fluid S 1 containing the magnetic substance P to flow from the outside of the bore 2 a toward the magnetic field center G side and the raw fluid S 1 on the magnetic field center G side. A magnetic separation unit M that magnetically separates the magnetic substance P, and a purification fluid S2 is allowed to flow from the magnetic separation part M in a direction opposite to the inflow direction of the raw fluid S1 and the direction in which the magnetic substance P is attracted by magnetic force, and is discharged out of the bore 2a. The outflow passage 12b and the accumulation portion Q for accumulating the magnetic substance P magnetically separated by the magnetic separation portion M are formed in series.

The cross-sectional area of the outflow path 12b is set larger than that of the inflow path 12a, but may be the same. In any case, the cross-sectional area of the magnetic separation portion M from the inner end of the inner cylinder 4 to the outer cylinder bottom wall 3a is the inner cylinder. 4 is larger than the cross-sectional area.
The extraction tube 5 is a thin tube, and is inserted inward from the outer end side of the inner cylinder 4, and its tip protrudes from the inner end opening of the inner cylinder 4 to the accumulation portion Q side, and the tip is the outer cylinder bottom A funnel-shaped suction port 5a that opens toward the wall 3a and extends toward the outer cylinder bottom wall 3a is formed.

  The distal end suction port 5 a of the extraction tube 5 is disposed in the accumulation field Q at a substantially magnetic field center G. That is, the tip suction port 5a is disposed above or in front of the magnetic field center G, is separated from the outer cylinder bottom wall 3a, and is disposed at a position where the magnetic substance P accumulated in the accumulation portion Q is easily attracted. Has been. Although the tip suction port 5a may be cut off, the funnel shape is opposed to the outer cylinder bottom wall 3a over a wider area, and can effectively suck the magnetic substance P.

  The extraction tube 5 is attached to the inner tube 4 by a tube fitting 8 provided between the extraction tube 5 and the inner tube 4. The pipe fitting 8 is constituted by the lid member 17 penetrating the outer end side of the extraction pipe 5 positioned outside the bore 2a, and an inner cylinder flange 4b provided on the outer periphery of the outer end of the inner cylinder 4. The lid member 17 is detachably fixed to the inner cylinder flange 4b, thereby fixing the position of the extraction pipe 5 with respect to the inner cylinder 4 and closing the upper end of the gap between the two.

  The outer cylinder 3 can be inserted into and removed from the superconducting magnet 2 and the insertion position can be adjusted by changing the thickness of the spacer 22, and the inner cylinder 4 can be inserted into and removed from the outer cylinder 3. The insertion position can be adjusted by changing the thickness of the spacer 23, and the extraction pipe 5 can be inserted into and removed from the inner cylinder 4, and the position of the inner cylinder 4 can be changed or the inner cylinder flange can be changed. By inserting a spacer between 4b and the lid member 17, the insertion position of the extraction tube 5 can also be adjusted.

Therefore, the magnetic separation tool 1A can be configured separately from the superconducting magnet 2 and can be inserted and mounted in the superconducting magnet 2, and the extraction tube 5, the inner cylinder 4 and the like can be replaced.
Next, a magnetic separation method in the magnetic separation apparatus 11 incorporating the magnetic separation tool 1A will be described.
A magnetic separator 1A is mounted in the bore 2a of the superconducting magnet 2, and a magnetic field is generated by the superconducting magnet 2. The raw fluid S1 containing the magnetic substance P to which magnetism is imparted flows from the intake 4a of the inner cylinder 4, and the inside of the inner cylinder 4 and the outer cylinder 3 is filled with the fluid S. When the raw fluid S1 further flows in continuously, the raw fluid S1 flows through the inflow passage 12a in the inner cylinder 4 toward the outer cylinder bottom wall 3a of the outer cylinder 3, and the magnetic separation part on the magnetic field center G side. By entering the M and flowing from the magnetic separation part M to the outflow passage 12b in the outer cylinder 3, while the flow direction of the fluid S is reversed during this time, and by accumulating in the magnetic separation part M The flow rate of the raw fluid S1 is temporarily stopped or extremely low.

When the raw fluid S1 is temporarily stopped or extremely slow, it is superconducting with respect to the behavior of the fluid S while it reaches the magnetic separation part M from the inner cylinder 4 and stays in the magnetic separation part M. The magnetic substance P is caused to move differently by the magnetic force of the magnet 2 and separated from the fluid S, and the separated magnetic substance P is further drawn from the magnetic separation part M to the magnetic field center G side and accumulated in the accumulation part Q.
The magnetic substance P in the accumulation part Q is directed in the direction in which the magnetic field strength is stronger due to the magnetic force, that is, in the center of the highest bore 2a in the axial center and near the inner peripheral surface of the bore 2a. Pulled and accumulated, and accumulated along the highest magnetic field part F1 and also accumulated in the high magnetic field part F2, so that the magnetic substance P forms a wall in the accumulation part Q. The bottom wall 3a is filled.

The fluid S in the magnetic separation part M is pushed out as the purified fluid S2 from the outflow path 12b between the inner cylinder 4 and the outer cylinder 3 when the raw fluid S1 enters the magnetic separation part M from the inflow path 12a. It will be a movement.
When the fluid S flows from the magnetic separation part M to the outflow passage 12b, the direction is opposite to the inflow direction of the raw fluid S1, opposite to the direction in which the magnetic substance P is attracted by the magnetic force, and the flow velocity is low. Therefore, the magnetic force overcomes the movement of the fluid S to reliably pull the magnetic material P away from the fluid S and reduce the amount of the magnetic material P discharged together with the purification fluid S2.

Due to the strong magnetic force of the magnetic field center G and the decrease in the flow velocity, the magnetic substance P in the raw fluid S1 is effectively accumulated in the accumulation portion Q on the front side of the outer cylinder bottom wall 3a and opens at or near the magnetic field center G. The accumulated magnetic substance P is sucked in a concentrated state and taken out of the superconducting magnet 2 from the suction port 5a at the tip of the extraction tube 5 that is in operation.
In the magnetic separator 1A, when the cross-sectional area of the inflow passage 12a and the cross-sectional area of the outflow passage 12b are set to be substantially the same, the flow rates of the raw fluid S1 and the purified fluid S2 are substantially the same, and the fluid S is directional. Even if a slight turbulence occurs, the air flows from the inflow path 12a to the outflow path 12b. This is a flow caused by a water head difference caused by the intake port 4a and the discharge port 3b.

The flow due to the head difference hardly affects the magnetic substance P in the magnetic separation part M and the accumulation part Q, and the magnetic separation part M has an outer cylinder having a strong magnetic field strength. A pulling force in the direction toward the bottom wall 3a acts, and in the accumulation portion Q, a force attracting the magnetic substance P toward the magnetic field center G side acts.
If the magnetic substance P that accumulates in the accumulation part Q increases and accumulates up to the magnetic separation part M, the flow of the fluid S will be hindered. By continuously taking out the integrated magnetic substance P, the raw fluid S1 can be continuously processed.

A magnetic substance such as a magnetic bead on which a ligand capable of specifically binding a target substance is supplied to a culture solution of cells or microorganisms, or other extract, and supplied to the magnetic separation device 11 as a raw fluid S1. .
Since the magnetic separation device 11 has a strong magnetic field in the magnetic separation unit M and can separate the magnetic substance P in a short time, and has a gentle magnetic field gradient, it can suppress the aggregation of the magnetic substance P as much as possible. Re-dispersion can easily occur.

In the purification of proteins and enzymes, there is a target substance that has only a weak binding force with respect to a ligand immobilized on a magnetic substance, and excessive external force that causes the removal of the binding is prohibited. 11, the superconducting magnet 2 can generate a very high magnetic field,
Even if the supply of the fluid S is increased, the flow of the fluid S from the inflow path 12a to the outflow path 12b through the accumulation portion Q is gentle, so that there is little aggregation and no excessive stress is applied. It can be re-dispersed without applying extra external force. These can contribute to high purification efficiency.

In the second embodiment shown in FIGS. 3 and 4, a horizontal magnetic separation device 11 is shown, and the superconducting magnet 2 is disposed horizontally so that the axis of the bore 2a is horizontal, and the superconducting magnet 2 is superconducting. A magnetic separator 1B is inserted into the magnet 2 from the side so as to be freely inserted and removed.
The outer end 3 of the bottomed cylindrical outer cylinder 3 is closed at the outer end by a closing member 15 provided between the inner periphery of the outer end and the inner cylinder 4. The mounting tool 6 that is arranged horizontally in the bore 2a and is detachably mounted on the superconducting magnet 2 has a plurality of abutments that abut the outer periphery of the outer cylinder 3 at a plurality of positions in the axial direction. A plurality (three in the embodiment) of arcuate mounting plates 6d are connected by connecting plates 6e extending in the axial direction in a plurality of bores 2a in the circumferential direction.

The mounting tool 6 has an arc shape that receives a part of the outer periphery of the outer cylinder 3, but may be formed in a shape that surrounds the entire outer periphery of the outer cylinder 3.
The inner cylinder 4 formed in a cylindrical shape with openings at both ends is arranged so that the inner end opening faces the outer cylinder bottom wall 3 a at a distance, and the outer end is closed by a lid member 17 provided between the extraction pipe 5. ing.
A support 7 for attaching the inner cylinder 4 to the outer cylinder 3 includes a sealing member 15 with a seal on the outer end side located outside the bore 2a, and a plurality of axial directions on the inner end side located in the outer cylinder 3. A plurality of support pieces 19 are arranged at intervals in the circumferential direction so as not to block the outflow passage 12b having a substantially annular cross section between the inner cylinder 4 and the outer cylinder 3. ing.

A pipe fitting 8 for attaching the extraction pipe 5 to the inner cylinder 4 includes the lid member 17 penetrating the outer end side of the extraction pipe 5 located outside the bore 2a, and an attachment provided in the inner end of the inner cylinder 4. A plurality of the mounting pieces 20 are provided at intervals in the circumferential direction so as not to block the inflow passage 12a in the inner cylinder 4.
The other configuration of the magnetic separator 1B of the second embodiment is the same as that of the magnetic separator 1A. The extraction pipe 5 has the tip suction port 5a disposed on the magnetic field center G, and the lid member 17 and the mounting piece 20 are separated from each other. Can be adjusted.

  In the magnetic separator 1 </ b> B, the raw fluid S <b> 1 is caused to flow to the magnetic separation unit M, and the magnetic substance P is pulled by the magnetic field to the high magnetic field portion F <b> 2 of the magnetic separation unit M to be separated from the fluid S and accumulated in the accumulation unit Q. Since the purifying fluid S2 flows from the magnetic separation part M in the direction opposite to the inflow direction of the original fluid S1 and the direction in which the magnetic substance P is attracted by the magnetic force, the flow of the magnetic substance P by the purifying fluid S2 can be reduced. P can be reliably separated from the fluid S by magnetic force and accumulated in the accumulating portion Q.

In the third embodiment shown in FIGS. 5 and 6, a magnetic separation tool 1 </ b> C that can be used in a vertically-oriented or horizontal-type magnetic separation device 11 is shown, and a cylindrical body includes an outer cylinder 3 and an inner cylinder 4. The extraction tube 5 is disposed between the outer tube 3 and the inner tube 4 in parallel with each other, and a high gradient magnetic capturing member 25 is provided at the inner end of the inner tube 4.
Two extraction pipes 5 are provided by being displaced 180 degrees in the circumferential direction between the outer cylinder 3 and the inner cylinder 4, and may be one or three or more at equal intervals in the circumferential direction. The magnetic material from the position where the mouth 5a is disposed on the magnetic field center G and in the vicinity of the inner peripheral surface of the outer cylinder 3 at the highest magnetic field site F1 of the superconducting magnet 2 and the magnetic material P is accumulated most in the accumulation portion Q. It arrange | positions so that P can be attracted | sucked.

The high-gradient magnetic capture member 25 is made of stainless steel and has a windmill shape. A large number of thin wires 25b are implanted on the outer periphery of the cylindrical shaft 25a at regular intervals in the axial direction and the circumferential direction. The thin wire 25b has a maximum diameter at the center of the cylinder shaft 25a and a minimum at both ends, and the arrangement shape of the tip is a substantially spherical shape.
The high gradient magnetic capture member 25 is rotatably supported by a bearing portion 26 having shaft portions 25c at both ends of a cylindrical shaft 25a projecting from the inner tube 4 toward the outer tube bottom wall 3a. Is inserted on the inner end side of the inner cylinder 4 and arranged so as to approach the magnetic field center G from the inner cylinder 4 side to the outer cylinder bottom wall 3a side.

When the high gradient magnetic capturing member 25 is placed in the magnetic field of the superconducting magnet 2, a very large magnetic gradient can be created on the surface of the stainless thin wire 25b, and the raw fluid S flowing in the inner cylinder 4 can be produced.
1 is distributed, the magnetic substance P contained therein is attached and captured, and the magnetic substance P is magnetically separated from the raw fluid S1.
When the magnetic substance P adhering to the stainless steel thin wire 25b is gradually accumulated and becomes larger, it is caused to flow by the hydraulic pressure of the raw fluid S1, and passes through the magnetic separation part M on the magnetic field center G side to accumulate part Q. Is accumulated. Further, when an imbalance occurs in the accumulated amount of the adhering magnetic substance P and the high-gradient magnetic capturing member 25 rotates, the stainless thin wire 25b rotates from the inner cylinder 4 side to the magnetic separation part M side, and reaches the magnetic field center G. When approaching, the large magnetic substance P is delivered by the strong magnetic force, and the magnetic substance P is accumulated in the accumulation part Q on the front side of the outer cylinder bottom wall 3a.

  In the magnetic separation tool 1C of the third embodiment, when the raw fluid S1 reaches the magnetic separation part M from the inflow path 12a, the magnetic substance P is separated and captured from the fluid S by the magnetic force of the high gradient magnetic capturing member 25 and The accumulated magnetic substance P is separated from the high gradient magnetic capturing member 25 and accumulated in the highest magnetic field part F1 and the high magnetic field part F2 of the accumulation part Q, and the flow of the purified fluid S2 to the outflow path 12b is also the original fluid Since the inflow direction of S1 and the direction in which the magnetic substance P is attracted by the magnetic force are reversed, the magnetic substance P can be reliably separated by the magnetic force. In addition, since a plurality of extraction tubes 5 are arranged at the highest magnetic field site F1 in the accumulating portion Q, the suction efficiency of the magnetic substance P can also be increased.

  In the fourth embodiment shown in FIG. 7, a magnetic separator 1 </ b> D that can be used in a vertically-oriented or horizontally-placed magnetic separator 11 is shown, and the cylinder includes an outer cylinder 3 and an inner cylinder 4. The outer cylinder bottom wall 3a of the outer cylinder 3 is arranged away from the magnetic field center G, the extraction pipe 5 is arranged in the inner cylinder 4 so as to be shifted (or concentrically) from the cylinder center, and its tip is An annular (or C-shaped) suction portion 5b is formed, and a plurality of suction ports 5a are formed on the outer peripheral surface of the suction portion 5b.

  In the magnetic separator 1D of the fourth embodiment, the capacity of the accumulating part Q is large, and the raw fluid S1 reaches the magnetic separation part M from the inflow path 12a and accumulates in the magnetic separation part M and then flows to the outflow path 12b. The magnetic material P flows in the direction opposite to the inflow direction of the original fluid S1, and the magnetic substance P is separated from the fluid S by the magnetic force in the magnetic separation unit M and is accumulated in a large amount in the accumulation unit Q. The magnetic substance P is efficiently sucked from the suction port 5a.

In the magnetic separator 1D, the extraction pipe 5 may be rotated around the cylinder center of the inner cylinder 4 so that the magnetic substance P in the entire accumulation section Q can be more reliably collected.
In the fifth embodiment shown in FIG. 8, a magnetic separator 1 </ b> E that can be used in a vertically-oriented or horizontal-type magnetic separator 11 is shown, and the cylinder includes an outer cylinder 3 and an inner cylinder 4. The extraction tube 5 extends from the outer cylinder bottom wall 3a of the outer cylinder 3 in the opposite direction to the inner cylinder 4 side, and the outer cylinder 3 and the inner cylinder 4 are provided with high gradient magnetic capture members 25, respectively. It has been.

The outer cylinder bottom wall 3a of the outer cylinder 3 has a conical concave shape, that is, a shape in which the magnetic substance P accumulated in the accumulating portion Q is collected from the outer peripheral portion to the central portion as much as possible. The extraction pipe 5 has a suction port 5a opened at the center of the outer cylinder bottom wall 3a, and extends from the outer cylinder bottom wall 3a in the opposite direction to the inner cylinder 4.
Each of the high-gradient magnetic capture members 25 is formed in a circular mesh shape with a large number of fine stainless steel wires, can create a large magnetic gradient in the magnetic field of the superconducting magnet 2, and the raw fluid S1 can flow. The first high gradient magnetic capture member 25A is attached to the inner end of the inner cylinder 4, and the second high gradient magnetic capture member 25B is attached to the outer cylinder 3 before the outer cylinder bottom wall 3a.

The first high-gradient magnetic capturing member 25A attaches and captures the magnetic substance P from the raw fluid S1 flowing through the inflow path 12a, and when the captured magnetic substance P is gradually accumulated and becomes larger, Flowed by hydraulic pressure, it is captured by a second high gradient magnetic capture member 25B that creates a larger magnetic gradient.
The magnetic separation tool 1E is provided with a high gradient magnetic capture member 25 so as not to give an excessive external force that causes a disconnection in the purification of a protein or enzyme containing a target substance having only a weak binding force. It is preferable to remove it.

When the magnetic substance P captured by the second high gradient magnetic capture member 25B is gradually accumulated and becomes larger, the second high gradient magnetic capture member 25 is caused by the fluid pressure of the raw fluid S1, the suction force of the extraction pipe 5, and the like.
It is separated from B, accumulated in the accumulating portion Q by a strong magnetic force on the magnetic field center G side, and attracted by the extraction tube 5. The purified fluid S2 after separation of the magnetic substance P flows out in the opposite direction to the raw fluid S1 through the outflow passage 12b between the outer cylinder 3 and the inner cylinder 4.

  In the magnetic separator 1E of the fifth embodiment, the flow directions of the raw fluid S1 and the purified fluid S2 are substantially the same as those of the magnetic separator 1D. However, the magnetic material is separated from the raw fluid S1 by the two-stage high gradient magnetic capture member 25. P can be magnetically separated, and the magnetic material P accumulated and enlarged in the high gradient magnetic capture member 25 can be accumulated on the center side of the conical concave outer cylinder bottom wall 3a of the accumulating portion Q. The magnetic substance P can be efficiently sucked by the extraction tube 5 from the center side of the bottom wall 3a.

  In the sixth embodiment shown in FIG. 9, a magnetic separator 1 </ b> F that can be used mainly in a horizontal type magnetic separator 11 is shown, and a pair of opposed superconductor magnets 2 are bored into the bore 2 a. A magnetic separator 1F is inserted and arranged. The cylinder of each magnetic separation tool 1F has a double cylinder structure having an outer cylinder 3 and an inner cylinder 4, the outer cylinder bottom wall 3a of the outer cylinder 3 has a conical convex shape, and the extraction pipe 5 has a bent end. Has been.

Both magnetic separation tools 1F face the outer cylinder bottom wall 3a of the outer cylinder 3 across the magnetic field center G, and use the magnetic field from the magnetic field center G one by one to the magnetic separation part M in the outer cylinder 3. Magnetic separation is performed, and the separated magnetic substance P is accumulated in the accumulation portion Q on the highest magnetic field site F1 side. Coupled with the fact that the outer cylinder bottom wall 3a has a conical convex shape, the magnetic substance P is collected on the outer peripheral side of the accumulation portion Q.
The extraction pipe 5 is arranged concentrically with the outer cylinder 3 and the inner cylinder 4, but its tip is bent and inclined toward the inner peripheral surface of the outer cylinder 3, and the suction port 5a at the tip is the outer cylinder bottom wall 3a. The magnetic material P is disposed on the outer peripheral side of the magnetic field P and disposed so as to be able to efficiently attract the magnetic substance P accumulated in the highest magnetic field site F1.

The magnetic separation device 11 of the sixth embodiment can double the magnetic field of the superconducting magnet 2, and both magnetic separation tools 1F may perform the magnetic separation by flowing the same raw fluid S1 from the inner cylinder 4. However, the purified fluid S2 after magnetic separation of the magnetic substance P by one magnetic separation tool 1F can be supplied to the other magnetic separation tool 1F as a raw fluid S1 to constitute a two-stage magnetic separation device.
In the case of the magnetic separator 1F, the magnetic substance P in the accumulating portion Q is easily accumulated in a ring shape. Therefore, a plurality of extraction tubes 5 are formed as in the third embodiment, or in a ring shape as in the fourth embodiment. It is preferable to do.

  In the seventh embodiment shown in FIG. 10, a magnetic separator 1G that can be used in a vertically-oriented or horizontal-type magnetic separator 11 is shown, and the cylinder has no distinction between the inner and outer cylinders, and the bottomed cylinder 30 is partitioned by a partition wall 31 from the outer end to the middle (before the magnetic field center G) to form the inflow path 12a and the outflow path 12b, and the magnetic separation section M and the stacking section are located on the far side where the partition wall 31 does not exist. Q.

  That is, the inflow path 12a for allowing the raw fluid S1 to flow from the outside of the bore 2a toward the magnetic field center G side into the cylinder inserted from the outside of the superconducting magnet 2 toward the magnetic field center G in the bore 2a, and the magnetic field center G The magnetic separation part M that magnetically separates the magnetic substance P from the raw fluid S1 on the side, and the purified fluid S2 is caused to flow in a direction opposite to the inflow direction of the raw fluid S1 and the direction in which the magnetic substance P is attracted by the magnetic force from the magnetic separation part M And the outflow path 12b discharged | emitted out of the bore 2a and the accumulation | aggregation part Q in front of the bottom wall 30a in the back of the magnetic separation part M are formed.

  The partition wall 31 is arranged in the diametrical direction in the bottomed cylindrical body 30, and the inflow passage 12a and the outflow passage 12b are formed in a semicircular flow passage having the same cross-sectional area. Since the partition wall 31 and the bottom wall 30a of the bottomed cylindrical body 30 are separated from each other, the flow of the raw fluid S1 flowing through the inflow path 12a is reversed to the outflow path 12b. A turn-around point through which the purification fluid S2 flows is formed, and this turn-back portion is a magnetic separation part M.

The magnetic substance P contained in the raw fluid S1 is pulled by the magnetic separation part M by the magnetic force of the highest magnetic field part F1 and the high magnetic field part F2, is separated from the fluid S, and is collected along the inner peripheral surface of the accumulation part Q. As the accumulation proceeds, the cross-sectional drum shape as shown in FIG. 10 is obtained, and further, the bottom wall 30a far from the magnetic field center G is accumulated.
The magnetic substance P accumulated in the accumulation part Q may be sucked out by inserting the extraction tube 5 from the upper part of the bottomed cylindrical body 30 as in the above embodiments, but the amount of the raw fluid S1 to be processed is small. If the amount is small, the magnetic separation tool 1G itself can be removed from the magnetic separation device 11 to recover the magnetic substance P.

In the present invention, the shape of each member and the positional relationship between the front, rear, left, and right in the embodiment are best configured as shown in FIGS. However, it is not limited to the said embodiment, The member of each embodiment and a structure can be variously deformed, and a combination can also be changed.
For example, the magnetic separation tool 1 is formed by bending one cylindrical body into a U shape or a U shape, forming a magnetic separation portion M and an accumulation portion Q in the middle, and an inflow path 12a and an outflow path 12b on both sides. May be formed.

Further, the extraction tube 5 may be connected to the accumulation portion Q from the outside of the diameter without being arranged inside the cylinder or the outer cylinder 3.
The magnetic separator 11 may be a room temperature electromagnet or a permanent magnet that can generate a strong magnetic field in place of the superconducting magnet 2.

DESCRIPTION OF SYMBOLS 1 Magnetic separator 2 Superconducting magnet 2a Bore 3 Outer cylinder 3a Outer cylinder bottom wall 3b Outlet 4 Inner cylinder 4a Inlet 5 Extraction pipe 5a Inlet 11 Magnetic separation device 12 Channel 12a Inflow path 12b Outflow path 25 High gradient magnetism Capturing member 30 Bottomed cylinder F Magnetic field part G Magnetic field center H Magnetic field line M Magnetic separation part P Magnetic substance Q Accumulation part S Fluid S1 Raw fluid S2 Purification fluid

Claims (9)

The raw fluid (S1) is directed from the outside of the bore (2a) to the magnetic field center (G) side into the cylinder inserted from the outside of the superconducting magnet (2) to the magnetic field center (G) side in the bore (2a). An inflow path (12a) for inflow, a magnetic separation part (M) for separating the magnetic substance (P) from the raw fluid (S1) by magnetic force, and a magnetic substance (P) separated by the magnetic separation part (M) as a magnetic field A collecting part (Q) that accumulates in the vicinity of the center (G), and a purified fluid (in a direction opposite to the inflow direction of the raw fluid (S1) from the magnetic separation part (M) and the direction in which the magnetic substance (P) is attracted by magnetic force ( Forming an outflow passage (12b) through which S2) flows and is discharged out of the bore (2a) ;
The cylinder has an inner / outer double cylinder structure, and an outer cylinder (3) having a bottomed cylindrical shape that forms the magnetic separation part (M) and the accumulation part (Q) on the outer cylinder bottom wall (3a) side; An inner cylinder (4) which is inserted into the outer cylinder (3) and forms the inflow passage (12a), and an inner peripheral surface of the outer cylinder (3) and an outer peripheral surface of the inner cylinder (4), The outflow passage (12b) is formed between the magnetic separators. The raw fluid (S1) is directed from the outside of the bore (2a) to the magnetic field center (G) side into the cylinder inserted from the outside of the superconducting magnet (2) to the magnetic field center (G) side in the bore (2a). An inflow path (12a) for inflow, a magnetic separation part (M) for separating the magnetic substance (P) from the raw fluid (S1) by magnetic force, and a magnetic substance (P) separated by the magnetic separation part (M) as a magnetic field A collecting part (Q) that accumulates in the vicinity of the center (G), and a purified fluid (in a direction opposite to the inflow direction of the raw fluid (S1) from the magnetic separation part (M) and the direction in which the magnetic substance (P) is attracted by magnetic force ( Forming an outflow passage (12b) through which S2) flows and is discharged out of the bore (2a);
The cylindrical body has an extraction tube (5) having a magnetic substance (P) that is magnetically separated in the magnetic separation part (M) and collected in the accumulation part (Q). Ingredients. The cylindrical body has a conical concave bottom wall (3a) in the accumulating portion (Q), and the magnetic substance (P) magnetically separated in the magnetic separating portion (M) and accumulated in the accumulating portion (Q). The magnetic separator according to claim 1 or 2, characterized in that an extraction tube (5) for suction and extraction is connected to the center side of the bottom wall (3a) . The magnetic separator (1) according to any one of claims 1 to 3 is inserted into a bore (2a) of a superconducting magnet (2) and is detachably mounted. Magnetic separation device. The magnetic separator (1) according to claim 3 is inserted from one end of the bore (2a) of the superconducting magnet (2) to the magnetic field center (G) side and is detachably mounted, and the extraction tube (5) magnetic separation apparatus characterized in that it extends to the other end of the bore (2a). A pair of magnetic separators (1) according to any one of claims 1 to 3 are inserted oppositely from both ends of the bore (2a) of the superconducting magnet (2) to the magnetic field center (G) side. Magnetic separation device characterized by being arranged . The raw fluid (S1) is directed from the outside of the bore (2a) to the magnetic field center (G) side into the cylinder inserted from the outside of the superconducting magnet (2) to the magnetic field center (G) side in the bore (2a). The purified fluid (S2) from which the magnetic substance (P) is separated by separating the magnetic substance (P) from the original fluid (S1) by separating the magnetic substance (P) by a magnetic force and collecting it in the collecting part (Q) near the magnetic field center (G). The raw fluid (S1) flows in the direction opposite to the direction in which the magnetic material (P) is attracted by the magnetic force and flows out of the bore (2a), and is accumulated by the magnetic force in the accumulation portion (Q) in the cylinder. The magnetic separation method, wherein the magnetic substance (P) is sucked out by the extraction tube (5) . The raw fluid (S1) is directed from the outside of the bore (2a) to the magnetic field center (G) side into the cylinder inserted from the outside of the superconducting magnet (2) to the magnetic field center (G) side in the bore (2a). The purified fluid (S2) from which the magnetic substance (P) is separated by separating the magnetic substance (P) from the original fluid (S1) by separating the magnetic substance (P) by a magnetic force and collecting it in the collecting part (Q) near the magnetic field center (G). The inflow direction of the raw fluid (S1) and the magnetic substance (P) are caused to flow in a direction opposite to the direction in which the magnetic substance (P) is attracted by the magnetic force and flow out of the bore (2a).
The raw fluid (S1) and the purified fluid (S2) flowing in the opposite direction to the raw fluid (S1) are divided into an inlet (4a) for the raw fluid (S1) and an outlet (3b) for the purified fluid (S2). The magnetic separation method is characterized by causing the fluid to flow by the difference in water head . The accumulating part (Q) is conically concave, and the magnetic substance (P) is collected from the outer peripheral part of the cylindrical body to the central side, and the magnetic substance (P) is collected from the central side through the extraction pipe (5) to the raw fluid (S1). The magnetic separation method according to claim 7, wherein the magnetic separation method is taken out in an inflow direction .

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