JP5006790B2 - 2次元電気泳動による生体分子の同時分離に関する方法及び装置 - Google Patents

2次元電気泳動による生体分子の同時分離に関する方法及び装置 Download PDF

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JP5006790B2
JP5006790B2 JP2007542164A JP2007542164A JP5006790B2 JP 5006790 B2 JP5006790 B2 JP 5006790B2 JP 2007542164 A JP2007542164 A JP 2007542164A JP 2007542164 A JP2007542164 A JP 2007542164A JP 5006790 B2 JP5006790 B2 JP 5006790B2
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matrix
electrophoresis
electrode
dimensional electrophoresis
biomolecules
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JP2008522144A5 (https=
JP2008522144A (ja
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ミッリオニ・レナト
グアダグニノ・アントニオ
ミッゾ・マニュエラ
バリナ・エリサ
サラタ・トッマソ
リゲッティ・ピエロジオロジオ
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エレットロフォ エス.エイ.エス.デイ ルッジェロ マッシモ アンド シー.
ジップ ソシエタ ア レスポンサビリタ リミタータ
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • G01N27/44773Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2007542164A 2004-11-26 2005-11-24 2次元電気泳動による生体分子の同時分離に関する方法及び装置 Expired - Fee Related JP5006790B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IT000301A ITPD20040301A1 (it) 2004-11-26 2004-11-26 Metodo ed apparato per la separazione simultanea di molecole biologiche mediante elettroforesi bidimensionale
ITPD2004A000301 2004-11-26
PCT/IB2005/003519 WO2006056861A1 (en) 2004-11-26 2005-11-24 Method and apparatus for the simultaneous separation of biological molecules by two dimensional electrophoresis

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JP2008522144A JP2008522144A (ja) 2008-06-26
JP2008522144A5 JP2008522144A5 (https=) 2012-03-29
JP5006790B2 true JP5006790B2 (ja) 2012-08-22

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JP2007542164A Expired - Fee Related JP5006790B2 (ja) 2004-11-26 2005-11-24 2次元電気泳動による生体分子の同時分離に関する方法及び装置

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US (1) US20080202934A1 (https=)
EP (1) EP1828760A1 (https=)
JP (1) JP5006790B2 (https=)
KR (1) KR20070089918A (https=)
CN (1) CN101111762B (https=)
CA (1) CA2589232A1 (https=)
IT (1) ITPD20040301A1 (https=)
WO (1) WO2006056861A1 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN105498540A (zh) * 2015-12-08 2016-04-20 北京大学第一医院 一种具有浓缩功能的电泳仪
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894590B1 (en) 2018-12-10 2025-10-22 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
CN111560063B (zh) * 2020-05-12 2022-11-01 蚌埠医学院 一种动物胰脏来源胰岛素原料药纯化装置及使用方法
CN116134308B (zh) 2020-05-19 2025-05-27 10X基因组学有限公司 电泳盒和仪器
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
EP4491742B1 (en) 2020-09-18 2026-03-04 10X Genomics, Inc. Sample handling apparatus and image registration methods
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844925A (en) * 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation
EP0497077B1 (de) * 1991-01-28 1996-07-17 Ciba-Geigy Ag Vorrichtung zur Vorbereitung von Proben insbesondere für Analysezwecke
US6071394A (en) * 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US6207031B1 (en) * 1997-09-15 2001-03-27 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
IT1309430B1 (it) * 1999-05-18 2002-01-23 Guerrieri Roberto Metodo ed apparato per la manipolazione di particelle per mezzo delladielettroforesi
ATE359502T1 (de) * 1999-07-16 2007-05-15 Applera Corp Vorrichtung und verfahren für hochdichte elektrophorese
US6328870B1 (en) * 2000-01-27 2001-12-11 Cbm Intellectural Properties, Inc. Electrophoresis gel running plate
GB0010957D0 (en) * 2000-05-05 2000-06-28 Novartis Ag Compound & method
EP1211510A1 (en) * 2000-11-29 2002-06-05 Proteologics, Inc. Methods and apparatus for nonlinear mobility electrophoresis separation
AUPR222300A0 (en) * 2000-12-21 2001-01-25 Life Therapeutics Limited Electrophoresis device and method
EP1379864A1 (en) * 2001-04-17 2004-01-14 Nextgen Sciences Ltd Electrophoretic separation system
GB0130235D0 (en) * 2001-12-18 2002-02-06 Deltadot Ltd Centrifugal spectrometer
JP3672910B2 (ja) * 2002-07-12 2005-07-20 太陽誘電株式会社 マイクロキャピラリーを有するディスク状光記録媒体、及び該ディスク状光記録媒体を用いた試料分析装置

Also Published As

Publication number Publication date
EP1828760A1 (en) 2007-09-05
US20080202934A1 (en) 2008-08-28
CN101111762A (zh) 2008-01-23
WO2006056861A1 (en) 2006-06-01
CA2589232A1 (en) 2006-06-01
CN101111762B (zh) 2011-01-12
KR20070089918A (ko) 2007-09-04
ITPD20040301A1 (it) 2005-02-26
JP2008522144A (ja) 2008-06-26

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