JP3208586B2 - Method for producing sheet-like bone filling material - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sheet bone used for fixing a root canal filling material, a bone filling material, bone cement, and pyorrhea.
The present invention relates to a method for producing a filler .

[0002]

2. Description of the Related Art Conventionally, as a curable composition using chitosan which is a natural polymer, an acidic aqueous solution in which chitosan is dissolved and a basic water-curable inorganic substance are mixed to make the entire system basic. There is a water-curable inorganic substance characterized in that:
This curable composition is based on the fact that the solubility of chitosan decreases in the basic region and becomes insoluble. As the basic water-hardened inorganic composition, lime, Portland cement, alumina cement and the like are used. About the basicity of the whole system after mixing, pH 9 or more is desirable. Other examples of the curable composition include an aqueous chitosan solution, hydroxyapatite, zinc oxide and / or zinc oxide.
Alternatively, a curable composition is produced by mixing magnesium oxide. Since the curable composition contains apatite, it has excellent affinity for teeth, bones, and the like, and has a characteristic of being cured near neutrality. (Japanese Unexamined Patent Publication No.
(See JP-A-208347) Chitosan is a calcium and calcium salt that is found in crustaceans, insect shells and fungi.
Present as a complex of proteins. This chitosan is obtained by deacetylating chitin and is a polysaccharide having an amino group. Furthermore, chitosan has antibacterial properties, and is used for plant fiber, cosmetics, porous carriers, adsorbents, soil conditioners, and the like. These are based on the fact that chitosan is a polyamine and easily forms a complex.

[0003] On the other hand, in the field of dentistry, dental cements such as coalescence of restorations, temporary filling, and root canal filling are used. Examples of dental cements include zinc linsan, zinc / eugenol, carboxylate, and the like. It is used by mixing powder and liquid at the time of use, and has a curing time of 4 to 10 minutes.

[0004]

However, any paste prepared by mixing a cement powder and a liquid in a dental cement has a pH of 2 to 4, that is, is acidic, and causes pulp damage in vivo. There is a problem.

[0005] In addition, granular apatite used as a bone filling material moves from the initial filling position,
For example, there is a problem that gingiva is inflamed due to occlusal pressure by entering between gingiva and bone.

[0006] Furthermore, the curable composition containing the apatite granules described above is not effective for bone formation because the granules may move and be excluded.

[0007] Therefore, an object of the present invention is to provide a non-toxic substance in the living body, a product near the neutrality after the completion of the chemical reaction, to prevent the recurrence of periodontal disease, An object of the present invention is to provide a sheet-shaped bone filling material that is excellent in bone formation without causing inflammation.

[0008]

According to the present invention, an aperture
Mixed with chitosan sol dissolved by acid
A mixing step of forming a mixture, and forming the mixture into a sheet.
Sheet forming process to make sheets, sodium chloride, heavy carbon
Sodium acid, sodium polyphosphate, calcium oxide
By a solution of at least one compound selected from
And a neutralizing step of neutralizing the sheet.
Thus, a method for producing a sheet-like bone filling material can be obtained.

Further, according to the present invention, animal bone is calcined and
A crushing step of crushing the animal bone of the nature to produce animal bone meal;
Mixing the animal bone meal with chitosan sol dissolved by acid
Mixing to form a mixture, and forming the mixture into a sheet.
Sheet forming process to make a sheet, sodium chloride,
Sodium bicarbonate, sodium polyphosphate, calcium oxide
Solution of at least one compound selected from the group consisting of
And a neutralizing step of neutralizing the sheet.
Thus, a method for producing a sheet-like bone filling material can be obtained.

[0010] According to the present invention, apatite and dynamic
Bone bone and crush the mineral animal bone to produce animal bone meal
Pulverizing step, chitosa dissolved by the animal bone meal and acid
A mixing step of mixing a mixture with the sol to form a mixture;
Forming a sheet by forming a sheet into a sheet,
Sodium chloride, sodium bicarbonate, sodium polyphosphate
At least one selected from calcium oxide and calcium oxide
Neutralizing the sheet with a solution of the compound.
Thus, a method for producing a sheet-like bone filling material is obtained.

Further, according to the present invention, the sheet is provided
Of a sheet-shaped bone filling material including a cutting step of cutting to a size to be
A manufacturing method is obtained.

[0012]

[0013]

[0014]

[0015]

The sheet-shaped bone filling material is prepared by mixing a chemically synthesized apatite and a chitosan sol dissolved by an acid to form a mixture, and forming the mixture into a sheet to form a sheet. Also, the sheet is neutralized with an aqueous solution containing at least one of the group of Group I and Group II metals. In addition, the mixture is apatite, animal bone meal alone,
Or use them in combination. The sheet thus prepared has a pH in the range of 6 to 8, and can be cut to a desired size according to the size of the affected part. That is, the sheet-shaped bone filling material is inserted between the jaw bone and the gingiva after surgery for periodontal disease or the like, thereby preventing reconstruction of the jaw bone or recurrence of periodontal disease or the like.

[0016]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The sheet-like bone filling material of the present invention is an osteoconductive material. The osteoconductive material has a property of producing bone when a substance is filled near or in bone in the field of dental care.

A first embodiment of the sheet-like bone filling material of the present invention will be described below. The sheet-shaped bone filling material has a sheet formed by a mixture of apatite capable of forming a living bone and chitosan sol having an antibacterial action dissolved by an acid. The sheet is neutralized by a solution of a compound containing at least one element selected from the group consisting of Group I and Group II metal elements. As the apatite, chemically synthesized hydroxyapatite, α-tricalcium phosphate, β-tricalcium phosphate, or the like is used. When the sheet is neutralized with the aqueous solution, a sheet-like bone filling material having a predetermined strength is obtained. The solution containing the compound of the Group I metal element uses physiological saline, for example, at least one of sodium chloride, sodium bicarbonate, and sodium polyphosphate.
Calcium oxide is used as the compound containing a Group II metal element.

The method for producing the sheet-like bone filling material of the first embodiment will be described with reference to FIG.

[0019] and apatite, and mixing step S ₁₁ of mixing the chitosan sol prepared by dissolving the acid make mixture, a sheet-forming step S ₂₁ to make a sheet form the mixture into a sheet, I group metals and II with an aqueous solution containing at least one element compound selected from among the group of group metal element and a neutralization step S ₃₁ to neutralize the sheet. In addition, the sheet made in this manner is freely cut into a desired size according to the size of the affected part. That is, the sheet-shaped bone filling material is inserted between the jaw bone and the gingiva after surgery for periodontal disease or the like to prevent reconstruction of the jaw bone and recurrence of periodontal disease or the like.

Further, as a second embodiment, the sheet-shaped bone filling material has a sheet formed by mixing a chitosan sol dissolved by an acid and a calcined and pulverized inorganic animal bone powder into a sheet. ing. Also in this sheet, as described in the first embodiment, the sheet is neutralized by a solution of a compound containing a compound of at least one element selected from the group consisting of a group I metal and a group II metal element. By forming a mixture of animal bone meal and chitosan sol in the form of a sheet, a neutral and uniform cured product can be obtained. In particular, the present invention focused on the fact that calcined inorganic animal bone powder is about twice as fast as apatite in conducting biological bone. The animal bone meal described herein is obtained by crushing bones of non-human animals. For example, animal bone meal is selected from bones of animals living on land such as cow bones and pig bones and bones of animals living in the sea.
In the sheet-shaped bone filling material, kneaded mud is obtained by kneading the mixture and chitosan sol.

The manufacturing method in the second embodiment is as follows.
This is performed as shown in FIG. 800 ° C ~ 110
And 0 ℃ the firing 3-7 hours grinding step S ₁ to make the animal bone powder of the inorganic by milling, and mixing step S ₁₁ that by mixing the chitosan sol prepared by dissolving the animal bone powder and the acid make mixture, mixture the sheet forming step S ₂₁ to make a sheet formed into a sheet, to neutralize the sheet with a solution containing a compound of at least one element the sheet is selected from the group of group I metals and group II metal element and a neutralization step S _31. In addition, the sheet made in this manner is freely cut into a desired size according to the size of the affected part.

Next, as a third embodiment, the sheet-shaped bone filling material has a sheet formed of a mixture of apatite, animal bone powder, and chitosan sol dissolved by an acid. The sheet is neutralized with a solution containing a compound of at least one element selected from the group comprising Group I and Group II metal elements.

As a third embodiment, a method for producing a sheet-like bone filling material will be described below with reference to FIG. 80 animal bones
And 0 ° C. C. to 1100 was fired ° C. in 3-7 hours pulverizing step S ₁ to make only animals bone powder mineral, mixing step S make the animal bone powder, and apatite, a mixture by mixing the chitosan sol prepared by dissolving the acid _11, a sheet-forming step S ₂₁ that the mixture formed into a sheet making sheet, a sheet with an aqueous solution containing at least one element compound selected from the group of at least group I metals and group II metal element sheet and a neutralization step S ₃₁ to neutralize. In addition, the sheet made in this manner is freely cut into a desired size according to the size of the affected part.

The apatite granules containing the apatite granules shown in the first and third embodiments do not move or are excluded. That is, since the chitosan sol gels and fixes the apatite granules, an osteoid matrix is recognized at the time when the chitosan is absorbed by the living body, and the chitosan is replaced with bone.

When bovine bone meal is used as an inorganic animal bone powder, as described above, bovine bone powder has a characteristic that the speed of conduction of biological bone is higher than that of apatite.
The time for forming the bone of the living body is short. Therefore, when the ratio between the bovine bone meal and the apatite is shown, the first embodiment contains only the bovine bone meal without the apatite. In the third embodiment, the ratio of beef bone meal to apatite was 9%.
When 9 W%, bovine bone meal is 1 W%, and apatite 50
When it is W%, beef bone meal is 50 W%. 100 beef bone meal
μm or less, preferably 74 μm or less. Further, the kneaded mud hardens in the neutralizing agent solution in 3 to 60 minutes, and a sheet-like bone filling material having a pH in the range of 6 to 8 is obtained.

The acid used for chitosan may be any acid that dissolves chitosan. Specifically, as an acid,
Acetic acid, formic acid, lactic acid, malic acid, citric acid, adipic acid,
Tartaric acid, malonic acid and the like are used. With this acid,
The dissolved chitosan can be made into an acidic sol.

Next, 0.5 g of chitosan, 0.5 g of malic acid, 10 c of physiological saline were added to the sheet-like bone filling material of the present invention.
0.2 g of hydroxyapatite was mixed with the chitosan sol prepared in c above, neutralized in a CaO 2 W% solution, and the compressive strength was measured. As a result, the compression strength is 22.8 kg
/ Cm ² . The pH value of this sheet-shaped bone filling material is
7.46. The pH value when mixing 0.2 g of hydroxyapatite with a chitosan sol made with 0.5 g of chitosan, 0.5 g of malic acid and 10 cc of physiological saline was 3.49.

On the other hand, 0.5 g of chitosan, 0.5 g of malonic acid
g, chitosan sol made with 10 cc of physiological saline and 0.2 g of hydroxyapatite mixed with CaO
The solution was neutralized in a 2 W% solution and the compressive strength was measured. as a result,
The compressive strength was 30.4 kg / cm ² . The pH value of this sheet-shaped bone filling material was 7.32. In addition, 0.5 g of chitosan, 0.5 g of malonic acid, 10 c of physiological saline
When 0.2 g of hydroxyapatite was mixed with the chitosan sol prepared in c, the pH value was 3.03.

Also, 0.5 g of malic acid, malonic acid, citric acid and the like are dissolved in 10 cc of physiological saline or distilled water to dissolve 0.5 g of chitosan, and the amounts of various apatites and animal bone meal are as follows. 0.1 to 10 g can be added.

Further, in the observation of the structure, malonic acid 0.5
g, beef bone flour 0.2 g was kneaded into a chitosan sol made with chitosan 0.5 g, and a sheet neutralized with a CaO 2W% solution was peeled from the gingiva of an adult dog's jawbone. After cutting to a dimension of 5 mm in length and inserting, it was sutured. Four weeks later, the adult dog was sacrificed, and the tissue was observed. As a result, it was confirmed by eosin staining that bone had been formed at the sheet insertion portion.

[0031]

As described above, according to the sheet-shaped bone filling material of the present invention, the pH of the prepared cured product is in the range of 6 to 8, so that it is not suitable for living organisms. The product is a hardened product near neutrality, and has excellent bone formation such as root canal filling material, bone filling material, bone cement, fixation of pyorrhea, etc. without pulp disorders.

In addition, since the sheet-like bone filling material can be inserted into the affected part after cutting the sheet according to the size of the affected part,
It is possible to prevent the rebuilding of bones of the living body and the recurrence of periodontal disease and the like.

[Brief description of the drawings]

FIG. 1 is a process explanatory view showing a method for producing a sheet-like bone filling material according to a first embodiment of the present invention.

FIG. 2 is a process explanatory view showing a method for producing a sheet-like bone filling material according to a second embodiment of the present invention.

FIG. 3 is a process explanatory view showing a method for producing a sheet-like bone filling material according to a third embodiment of the present invention.

[Explanation of symbols]

S ₁ grinding process S ₁₁ mixing process S ₂₁ sheet forming process S ₃₁ neutralizing process

──────────────────────────────────────────────────続 き Continued on front page (58) Field surveyed (Int. Cl. ⁷ , DB name) A61L 27/00-27/58 A61C 13/08-13/107

Claims (4)

(57) [Claims] Apatite and chito dissolved by acid
A mixing step of mixing with a sun sol to form a mixture;
A sheet forming step of forming a sheet by forming the body in a sheet shape,
Sodium chloride, sodium bicarbonate, sodium polyphosphate
At least one selected from calcium oxide and calcium oxide
A neutralization step of neutralizing the sheet with a solution of a compound;
A method for producing a sheet-like bone filling material, characterized by comprising: 2. An animal bone is calcined to crush the inorganic animal bone.
Grinding process to produce animal bone meal, and
Mixing to make a mixture by mixing with dissolved chitosan sol
And forming the mixture into a sheet to form a sheet.
And sodium chloride, sodium bicarbonate,
Selected from sodium phosphite and calcium oxide
Neutralize the sheet with a solution of at least one compound
Bone filling comprising a neutralizing step
Method of manufacturing the agent. 3. An inorganic material comprising calcined apatite and animal bone.
A crushing step of crushing the animal bone to produce animal bone meal;
Mix and mix bone meal and chitosan sol dissolved by acid.
A mixing step of forming a unity, and forming the mixture into a sheet to form a sheet.
Sheet forming process to make a sheet, sodium chloride, bicarbonate
Sodium, sodium polyphosphate, calcium oxide
By a solution of at least one compound selected from
And a neutralizing step for neutralizing the sheet.
A method for producing a sheet-like bone filling material. 4. A cutting device for cutting said sheet into a desired size.
The sheet-shaped bone replacement according to claim 1, 2 or 3, further comprising a cutting step.
Method of manufacturing the agent.