JP3184868U - An LED excitation light source device capable of observing fluorescence in the same manner as a fluorescence microscope using an eyepiece tube of a bright field microscope. - Google Patents

Abstract

Provided is an LED excitation light source device that can accurately irradiate a target and can perform fluorescence observation similar to a fluorescence microscope using a bright field microscope at low cost.
An LED excitation light source unit (an LED light source installed in a housing 2) is obtained by removing an eyepiece lens using one of two normal eyepieces 1 of a bright field microscope (an upright microscope and an inverted microscope). 5 and the condensing lens 4 and the excitation filter 3) are set, and the fluorescence emitted by the target is observed through the observation emission filter 12 set on the other eyepiece 13.
[Selection] Figure 1

Description

  The present invention makes possible fluorescence observation similar to a fluorescence microscope by using an eyepiece tube of a bright field microscope and attaching an LED excitation light source to the eyepiece tube.

As a fluorescence observation method of biological samples, fluorescence observation is generally performed using an external excitation light source in a fluorescence microscope or bright field microscope. However, the fluorescence microscope is expensive and complicated to handle, while the bright field microscope is not used. In the case of an attached excitation light source, the target portion could not be excited precisely and the operability of the microscope was reduced.

The present invention makes it possible to accurately irradiate a target using the eyepiece tube of a bright-field microscope for the fluorescence observation that could not be obtained with an external excitation light source, and to perform the same fluorescence observation as a fluorescence microscope at low cost. It tries to solve it as possible.

The present invention solves the problem of the external excitation light source by attaching the LED excitation light source to the eyepiece tube of the bright field microscope and using the original optical path of the microscope to accurately excite the observation target.

The present invention is useful for research in the field of fluorescence observation because the bright field microscope can be easily and inexpensively transferred to a fluorescence microscope.

B) LED excitation light source unit of the present invention b) Emission filter unit for observation of the present invention

Using one of the two eyepiece tubes of bright field microscopes (upright microscope and inverted microscope), remove the eyepiece, set the LED excitation light source, and pass through the observation emission filter set on the other eyepiece The fluorescence emitted from the target can be observed with fluorescence.

Hereinafter, an embodiment will be described with reference to the accompanying drawings.
B) The excitation light source unit and the constant current power supply unit for the excitation light source set in the bright field microscope main body and the bright field microscope eyepiece tube unit. B) shows the observation emission filter set in the eyepiece tube unit.

1 Light field microscope main body (upright microscope or inverted microscope) and eyepiece tube mounting details 2 Excitation light source housing case 3 Excitation light source filter
4LED lens 5LED
6 LED board 7 Aluminum rod for heat radiation 8 DC cord for LED 9 Constant current power supply unit for LED 10 Objective lens 11 Fluorescence dyeing sample 12 Emission filter for observation
13 eyepiece (no light source inserted)

Claims (1)

Illumination light from the excitation light source unit (2) to (8) attached to the bright field microscope barrel unit (1) through the microscope optical path (10) below the objective lens (upright microscope) or on the objective lens (inverted microscope) (11) An LED excitation light source device capable of observing fluorescence by irradiating the fluorescently-stained target sample and passing the fluorescence emitted from the target through the observation emission filter (12).

Images