JP3146883U - Gel electrophoresis molding device - Google Patents

Abstract

A gel electrophoresis molding apparatus is provided.
Gradient gel electrophoresis with high accuracy and stable quality can be formed without generating bubbles during molding gel electrophoresis, and a long slit is held at the bottom end of the mounting piece set. When a gel outlet corresponding to each other is installed below the slit and the liquid gel is continuously driven by the roller of the collection tank and continuously injected from the gel outlet and the slit into the mounting piece set, The liquid gel injected into is pushed up horizontally by the liquid gel injected later, gradually injected from the bottom up into the mounting piece set, and the injected liquid gel is solidified to obtain a finished gel electrophoresis product. If two types of liquid base materials are inserted from the collecting groove, the liquid gel injected into the mounting piece set has a gradient.
[Selection] Figure 3

Description

  The present invention relates to a technical means of gel electrophoresis molding, and more particularly to a gel electrophoresis molding apparatus suitable for forming gradient gel electrophoresis.

  Electrophoresis devices are widely used in the fields of molecular biology, genetics and biochemistry, and gel electrophoresis is often faced with problems in that it is not easy to generate bubbles and control gradients in manufacturing.

  FIG. 1 is a diagram showing a well-known gel electrophoresis molding mode (1). According to this, a sheet-like accommodation space 91 to be used for solidification molding after liquid gel injection is formed between the two glass pieces of the mounting piece set 90, and the liquid gel injection method is the drop tube 100. After the liquid gel flowing from the upper end to the bottom end of the sheet-like storage space 91 reaches a predetermined height, the liquid gel is allowed to stand for a certain time to solidify, thereby obtaining a finished gel electrophoresis product. However, in such an injection method using a drop tube dropping means, bubbles are easily mixed during gel electrophoresis. In addition, when manufacturing a product for gradient gel electrophoresis, it is considerably troublesome and time consuming to drop a liquid gel having a gradient drop for each layer by a manual method.

  FIG. 2 is a diagram (2) showing a known embodiment of gel electrophoresis. In order to improve the problem that air bubbles are mixed during gel electrophoresis, in this gel electrophoresis manufacturing method, a plurality of gel injection tubes 200 are inserted into the bottom end of the sheet-like accommodation space 91, and the tube port of each gel injection tube 200 is inserted. Is located at the bottom end of the sheet-like accommodation space 91, and the liquid gel to be injected diffuses in such a manner as to push the liquid gel previously injected toward the both sides gradually upward, centering on the gel injection tube 200. However, such an injection method in which the gel injection tube 200 is inserted into the bottom end of the sheet-like accommodation space 91 improves the problem of bubbles generated by the above-mentioned drop tube dropping means, but the gradient gel electrophoresis is performed. At the time of performing, the liquid gel is extruded so as to protrude upward and to both sides around the mouth of the gel injection tube 200 to draw an arc shape, and therefore, the horizontal concentration change value that appears in gradient gel electrophoresis. Becomes inaccurate and forms a situation where the same horizontal height gel concentration or mixing ratio is different.

  The main object of the present invention is to provide a kind of gel electrophoresis molding apparatus, to prevent air bubbles from being contaminated after gel electrophoresis molding, and to correct the horizontal density change value of the gradient gel electrophoresis to be produced.

In order to achieve the above object, the gel electrophoresis molding apparatus of the present invention comprises:
A seat with a long gel outlet with an upward opening;
A positioning assembly installed around the upper periphery of the elongate gel delivery port;
A mounting piece set installed upright on the positioning assembly, wherein the mounting piece set includes a sheet-shaped storage space, and a long slit is retained at the bottom end of the sheet-shaped storage space, And the width of the long gel delivery port coincides with the slit, and the long gel delivery port is located below the slit,
Is included.

  The liquid gel is composed of two types of liquid substrates, and the seat is provided with a collection groove having a width corresponding to the width of the upper end of the gel delivery port, and the diameter of the collection groove is greater than that of the collection groove. A slightly smaller roller is provided, and two receiving grooves for injecting the two kinds of liquid base materials are provided above the collecting grooves, respectively, and a collecting groove having a long cross section and a width at the bottom end of each receiving groove. A channel matching the width of the liquid is provided, a peristaltic pump for controlling the injection port of each liquid substrate and the injection sequence is provided above each receiving groove, and a check valve is provided in one of the channels. It has been.

  The mounting piece set described above includes a front mounting piece and a rear mounting piece, and two spacers that are sandwiched between the front mounting piece and the rear mounting piece to form the sheet-like accommodation space.

  The positioning assembly has a positioning frame fixed on a seat body, push plates positioned on both front sides of the positioning frame, and a positioning groove for installing the mounting piece set is provided in front of the positioning frame. A long through hole connected to the gel delivery port is provided at the bottom of the positioning groove. There are two height adjustment grooves installed upright on the two sides of the front wall surface of the positioning groove, and a height control plate is provided in each height adjustment groove, and an upper recess is formed in the groove wall of the height adjustment groove. A lower recess is provided, and the height control plate is provided with a positioning protrusion corresponding to the upper recess and the lower recess. The above-described height control plate is provided with a convex extending piece that sandwiches the upper and lower portions of the rear mounting piece, and two pressure control cams that contact the upper end of the rear mounting piece are provided at the upper front end of the positioning groove.

  A connecting groove into which the positioning assembly is inserted vertically is provided around the above-mentioned gel delivery port, and a lateral positioning groove is provided on both sides of the positioning assembly, and the seat body is provided with each lateral positioning groove. A corresponding positioning handle is provided, and a locking plate is provided at the end of the positioning handle so as to rotate laterally and enter the lateral positioning groove.

  As a result, the present invention forms a sheet-shaped accommodation space for coagulation molding after liquid gel injection by an upright mounting piece set, and a long slit is reserved at the bottom end position of the sheet-shaped accommodation space of the mounting piece set. And a long gel delivery port whose width coincides with the lower side of the slit and whose opening is directed upward, and the liquid gel passes through the slit from the gel delivery port by the roller in the collection groove, and the bottom end of the sheet-like accommodation space The liquid gel injected earlier is pushed up horizontally by the liquid gel injected later, gradually injected from bottom to top into the sheet-like accommodation space, and after the injected liquid gel solidifies A finished gel electrophoresis product is obtained.

  In addition, in the present invention, the liquid gel continuously fed into the mounting piece set has a gradient by the above-described peristaltic pump, collecting groove, and roller. The gradient formation process is such that the first type liquid substrate is continuously sent to the collecting groove at a constant flow rate, and the roller is continuously rotated when the second type liquid substrate is further sent to the collecting groove. Two kinds of liquid substrates collected directly in the collecting groove are continuously delivered, so that the second kind of liquid substrate is. The liquid gel that is injected into the first type liquid substrate in a manner in which the mixing ratio is gradually increased, that is, the liquid gel that is continuously delivered forms a gradient by gradually increasing the second type liquid substrate mixing ratio.

  Compared with the prior art, the present invention uses a peristaltic pump to control the injection amount and injection timing of two types of liquid substrates, and continuously injects two types of liquid substrates that are injected by a rotating roller. As the liquid gel gradually rises in the sheet-like storage space, the concentration of the liquid gel gradually increases, and a gradient gel electrophoresis in which the concentration gradually increases from top to bottom can be formed. In addition, the present invention is applied to a gradient gel electrode in which the liquid gel is pushed up horizontally from bottom to top, and the liquid gel at the same horizontal altitude has the same concentration and mixing ratio, thereby achieving high accuracy and stable quality. Electrophoresis can be formed and no bubbles are generated during the production process.

  3 and 4 are a three-dimensional external view and a three-dimensional exploded view of the best embodiment of the device of the present invention, according to which the gel electrophoresis molding device of the present invention includes a seat 10, a positioning assembly 20 and a mounting. Includes a piece set 30. Among them, the positioning assembly 20 mounts the mounting piece set 30 and is fixed on the seat body 10, and the liquid gel is injected into the mounting piece set 30 by the seat body 10 to solidify the liquid gel, Gel electrophoresis is complete.

  As shown in FIGS. 4 and 5, the seat body 10 includes a long gel delivery port 11, and the opening of the gel delivery port 11 faces upward. A cylindrical collection groove 12 having a width corresponding to the upper end of the gel delivery port 11 is provided in the seat body 10, and a roller 13 having a diameter slightly smaller than that of the collection groove 12 is provided in the collection groove 12. Two storage grooves 14 for injecting different liquid base materials are provided in a portion above the collection groove 12, and the bottom end of each storage groove 14 has a long cross section and the width is the same as the collection groove 12. A matching channel 15 is provided, a peristaltic pump 16 for controlling the injection amount of each liquid substrate is connected above each receiving groove 14, and a check valve 151 is provided in at least one of the channels 15; A connecting groove 17 for inserting the positioning assembly 20 vertically is provided around the gel delivery port 11 of the seat body 10.

  Please refer to FIG. 4 and FIG. 7 is a three-dimensional exploded view of the best embodiment of the positioning assembly of the present invention. The positioning assembly 20 is pivotally mounted on both front sides of the positioning frame 21 which can be inserted into the connecting groove 17 in the vertical direction. The pressed plate 22 is included.

  A lateral positioning groove 211 is provided on both sides of the positioning frame 21, a positioning handle 18 corresponding to the lateral positioning groove 211 is provided on the seat body 10, and the end of the positioning handle 18 is rotated laterally. A locking plate 19 that can enter the lateral positioning groove 211 is provided.

  In front of the positioning frame 21. A positioning groove 23 for placing the mounting piece set 30 is provided. A long through hole 24 connected to the gel delivery port 11 is provided at the bottom of the positioning groove 23, and 2 on the front wall surface of the positioning groove 23. Two height adjustment grooves 25 installed upright are provided on the side, and a height control plate 26 is provided in each height adjustment groove 25, and an upper recess 251 and a lower recess 252 are formed in the groove wall of the height adjustment groove 25. The height control plate 26 is provided with one positioning convex portion 261 corresponding to the upper concave portion 251 and the lower concave portion 252, and the upper portion of the rear mounting piece 32 is provided at the bottom portion of the height control plate 26. A convex extending piece 262 that sandwiches the lower part of the positioning groove 23 is provided, and a pressure control cam 27 that contacts the upper end of the rear mounting piece 32 is provided at the upper front end of the positioning groove 23.

  As shown in FIGS. 4 to 6, the mounting piece set 30 is sandwiched between the front mounting piece 31, the rear mounting piece 32, and the two sides between the front mounting piece 31 and the rear mounting piece 32. The spacer 33 is included, an appropriate distance is maintained between the front mounting piece 31 and the rear mounting piece 32, a sheet-like accommodation space 34 is formed, and the bottom of the sheet-like accommodation space 34 of the placement piece set 30. A long slit 35 is retained at the end, and the aforementioned gel delivery port 11 has a width that matches the slit 35 and is positioned below the slit 35.

  As shown in FIG. 4 to FIG. 8, with the above-described configuration, the gradient gel electrophoresis using the present invention controls the injection amounts of two kinds of liquid substrates by two peristaltic pumps 16, and two kinds of liquid bases. The liquid base material is injected into the collecting groove 12 of the seat body 10. When the first type liquid substrate is continuously injected into the collecting groove 12 at a constant flow rate and the second type liquid substrate is further injected into the collecting groove 12, the roller 13 is continuously rotated to directly collect the collecting groove 12. The two kinds of liquid base materials are sent out, thereby forming a mode in which the mixing ratio is gradually increased in the second type liquid base material and injected into the first type liquid base material, that is, in the liquid gel that is continuously delivered. A gradient is formed by gradually increasing the second type liquid substrate.

  When the liquid gel is continuously injected from the slit 35 through the elongated through-hole 24 from the long and long gel delivery port 11, the liquid gel exhibits a horizontally rising shape, and continues to the mounting piece set 30. It enters the bottom end of the sheet-like accommodation space 34.

  As shown in FIG. 8, when the above-described liquid gel is continuously injected into the sheet-like accommodation space 34 of the mounting piece set 30, the previously injected liquid gel is pushed up horizontally by the subsequently injected liquid gel. As the concentration of the liquid gel to be injected later increases gradually, the concentration gradient gel electrophoresis is formed in which the concentration gradually increases from the top to the bottom. 9 and FIG. 10, after the liquid gel injection is completed, the pressure control cam 27 is pushed down to lower the rear mounting piece 32 together with the height control plate 26, and the bottom of the rear mounting piece 32 is long. The slit 35 can be closed by closing the scale-shaped through hole 24. At this time, after the liquid gel is solidified, the positioning handle 20 of the seat body 10 can be turned to release the positioning assembly 20, and the liquid gel can be released. Injection The rest piece set 30 permanently than the seat body 10 with the positioning assembly 20, thus it is possible to obtain a gradient gel electrophoresis in the finished product.

  Using the present invention, a liquid gel with a gradient is pushed up horizontally from bottom to top, so that liquid gels of the same horizontal height have the same concentration and mixing ratio, which results in high accuracy and stable quality Gradient gel electrophoresis can be obtained and no bubbles are generated during the manufacturing process.

It is a shaping | molding mode display figure (1) of well-known gel electrophoresis. It is a shaping | molding mode display figure (2) of well-known gel electrophoresis. It is a three-dimensional external view of the best embodiment of the device of the present invention. FIG. 2 is an exploded view of the best embodiment of the device of the present invention. 1 is a cross-sectional view of the best embodiment of the device of the present invention. It is a local enlarged view of FIG. 1 is an exploded view of a positioning assembly of the best embodiment of the device of the present invention. FIG. It is a liquid gel injection | pouring display figure of the best Example of the apparatus of this invention. It is a slit closing display of the best embodiment of the device of the present invention. FIG. 10 is a locally enlarged view of FIG. 9.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Seat 11 Gel delivery port 12 Collection groove 13 Roller 14 Accommodating groove 15 Channel 151 Check valve 16 Peristaltic pump 17 Connecting groove 18 Positioning handle 19 Locking plate 20 Positioning assembly 21 Positioning frame 211 Lateral positioning groove 22 Pushing plate 23 Positioning groove 24 long through hole 25 height adjustment groove 251 upper recess 252 lower recess 26 height control plate 27 pressure control cam 30 mounting piece set 31 front mounting piece 32 rear mounting piece 33 spacer 34 sheet-like accommodation space 35 slit

Claims (8)

In gel electrophoresis molding equipment,
A liquid body that is a seat body, has a long gel delivery port with an opening facing upward, has a cylindrical collection groove connected to the lower side of the gel delivery port, and forms a liquid gel from the collection groove. The seat body that is fed, a roller having a diameter smaller than the collection groove is installed in the collection groove, and the liquid substrate is sent to the gel delivery port by rotation of the roller;
A positioning assembly installed in the upper periphery of the gel delivery port;
A mounting piece set installed upright on the positioning assembly, wherein the mounting piece set has a sheet-like storage space, and the mounting piece set is elongated at the bottom end of the sheet-like storage space. Retaining the slit, the width of the slit coincides with the gel delivery port, and the liquid substrate is injected into the sheet-like accommodation space,
A gel electrophoresis molding apparatus comprising:   2. The gel electrophoresis molding apparatus according to claim 1, wherein the liquid gel is a mixture of two types of liquid base materials, and two containers for injecting the two types of liquid base materials above the collection groove. A gel electrophoresis molding apparatus, characterized in that a groove is provided, and a channel having a long cross section and a width matching the collection groove is formed at the bottom end of each receiving groove.   The gel electrophoresis molding apparatus according to claim 2, wherein a width of the collection groove coincides with the gel delivery port.   3. The gel electrophoresis molding apparatus according to claim 2, wherein a peristaltic pump is connected above each receiving groove, and each of the two peristaltic pumps injects a liquid substrate into the receiving groove. Electrophoresis molding device.   3. The gel electrophoresis molding apparatus according to claim 2, wherein a check valve is provided in one of the channels.   3. The gel electrophoresis molding apparatus according to claim 2, wherein the mounting piece set includes a front mounting piece, a rear mounting piece, and two spacers provided between both sides of the front mounting piece and the rear mounting piece. A gel electrophoresis molding device comprising: a sheet-like accommodation space formed between the front placement piece, the rear placement piece, and the two spacers.   The gel electrophoresis molding apparatus according to claim 6, wherein the positioning assembly includes a positioning frame fixed on a seat body, and push plates positioned on both front sides of the positioning frame, and is placed in front of the positioning frame. A positioning groove is provided for installation of the piece set, and a long through hole connected to the gel delivery port is provided at the bottom of the positioning groove, and two on the two sides of the front wall surface of the positioning groove. There are height adjustment grooves installed upright, a height control plate is provided in each height adjustment groove, an upper recess and a lower recess are provided in the groove wall of the height adjustment groove, and the height control plate Are provided with positioning protrusions corresponding to the upper and lower recesses, and the above-described height control plate is provided with a projecting extension piece that sandwiches the upper and lower parts of the rear mounting piece. Two suppression cams that come into contact with the upper end of the mounting piece are provided, Gel electrophoresis molding device.   2. The gel electrophoresis molding apparatus according to claim 1, wherein a connecting groove into which the positioning assembly is inserted vertically is provided around the gel delivery port, and lateral positioning grooves are provided on both sides of the positioning assembly. The seat body is provided with a positioning handle corresponding to each lateral positioning groove, and an end of the positioning handle is provided with a locking plate that can rotate laterally and enter the lateral positioning groove. A gel electrophoresis molding apparatus.

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