JP2866983B2 - Method for mycorrhizal fungus whitening - Google Patents
Method for mycorrhizal fungus whiteningInfo
- Publication number
- JP2866983B2 JP2866983B2 JP2231062A JP23106290A JP2866983B2 JP 2866983 B2 JP2866983 B2 JP 2866983B2 JP 2231062 A JP2231062 A JP 2231062A JP 23106290 A JP23106290 A JP 23106290A JP 2866983 B2 JP2866983 B2 JP 2866983B2
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- Prior art keywords
- root system
- seedling
- mycorrhizal
- root
- host
- Prior art date
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Description
【発明の詳細な説明】 (産業上の利用分野) 本発明はマツタケ、ホンシメジ等のような菌根性茸の
活物寄生菌の菌根菌を、寄主の植物の苗木の根に接種
し、これを種菌苗として寄主樹林内に植栽してシロ形成
を図る方法、並びに、育成箱に数本の寄主樹を植栽し、
その根に菌根菌を接種して、菌根を作りシロを形成させ
る方法に関する。DETAILED DESCRIPTION OF THE INVENTION (Industrial application field) The present invention inoculates the roots of seedlings of a host plant with mycorrhizal fungi of an active parasite of mycorrhizal mushrooms such as matsutake mushrooms and hon-shimeji mushrooms. Planting seed trees as seed fungi in the host tree forest to achieve white formation, and planting several host trees in the growing box,
The present invention relates to a method of inoculating mycorrhizal fungi into the roots to form mycorrhiza and form whites.
(従来の技術) マツタケ、ホンシメジ等は、それらの特定の寄主植物
の生きた根に寄生して菌根を作り、その菌根が一定以上
の量に達してシロが形成される。更に、このシロに、養
分、水分、温度の好適条件がととのう時、子実体が発生
する。(Prior art) Matsutake, hon-shimeji mushrooms and the like parasitize the living roots of their specific host plants to form mycorrhiza, and the mycorrhiza reaches a certain amount or more to form whites. Furthermore, when the suitable conditions of nutrients, moisture and temperature are satisfied, fruiting bodies are generated.
従って、マツタケ、ホンシメジ等活物寄生菌のシロを
人為的に形成し、子実体を栽培するに当たっては、胞子
播種法、シロ移植法などが試みられてきた外、確実性の
高い方法として、マツタケ、ホンシメジ菌等を、その寄
主植物の苗木の根に接種、感染させて、この苗木を種菌
苗として、寄主樹林内に植栽し、成木の寄主樹の根に二
次感染させることにより、シロを形成させる方法が考え
られた。Therefore, in order to artificially form shiro of matsutake mushrooms, hon-shimeji mushrooms and other active organisms and cultivate fruiting bodies, spore seeding methods, shiro transplantation methods, etc. have been tried, but matsutake mushrooms are highly reliable. , By inoculating roots of the seedlings of the host plant with H. shimeji, etc., and infecting the seedlings, planting the seedlings as seed fungus seedlings in a host tree forest, and secondary infecting the roots of the adult tree host tree. A method of forming the slab was considered.
この種菌苗作製には、培養菌糸を使って植菌する方法
と、自然のシロを使ってシロ拡大方向の先端に接触して
寄主植物苗を植栽して、この苗に自然感染させる方法と
がある。There are two ways to inoculate this seedling: a method of inoculating it with a cultured mycelium, a method of infecting the seedling by planting a host plant seedling by contacting the tip of the white growing direction using a natural white. There is.
(本発明が解決しようとする課題) しかし、この種菌苗を、寄主樹林内に植栽してシロ形
成を図るときに、特にマツタケ菌の場合、その成長が非
常に遅いため、シロを形成し、子実体発生に至るまでに
数年かかるものとされる。そのためこの間に、この種菌
苗の枯損する率が非常に高いことが分かってきた。(Problems to be solved by the present invention) However, when this inoculum seedling is planted in a host tree forest to achieve white formation, especially in the case of Matsutake fungi, the growth is very slow, so that white seeds are formed. It will take several years before fruiting occurs. Therefore, during this time, it has been found that the rate of withering of the seed fungus is extremely high.
そこで、種菌苗の枯損の原因は二つは考えられ、第一
は植栽した林内の日照不足。第二は菌根は透水性が悪く
水をはじくので、菌根の集団であるシロ内は、雨水が浸
透しにくくて乾燥するため、菌根を形成した根は、水分
や養分の吸収能が失われる。There are two possible causes for the death of the seed fungus, the first being lack of sunlight in the planted forest. The second is that mycorrhizas have poor water permeability and repel water, so the inside of the mycorrhizal population, Shiro, is hard to penetrate rainwater and dries, so the roots that have formed mycorrhiza have an ability to absorb moisture and nutrients. Lost.
これについて従来、マツタケ、シメジ等菌根菌のシロ
においては、菌根菌の生成する発根ホルモンのオーキシ
ン類や、サイトカイニン類が作用して、菌根を形成した
寄主樹の根は、テングス状に多数分岐し、細根量が増大
して、特有の形態をとることから、寄主樹は菌根菌の寄
生により、養水分の吸収が活発となりその成育が助長さ
れると考えられている。Conventionally, the roots of arbuscular mycorrhizal fungi such as matsutake and shimeji have been found to have a myxomycete-forming root auxin and cytokinins that act to form the mycorrhizal roots of the host tree. It is thought that the host tree absorbs nutrient water and promotes its growth due to the infestation of mycorrhizal fungi.
マツタケのシロでは菌糸がアカマツの根の表皮から細
胞の間に侵入し、一方では土中に広がって土中の水分や
養分を吸収してマツに送り、見返りとして糖分やビタミ
ンを受け取っている(「マツタケ山のつくり方」P3マツ
タケ研究懇話会編 創文刊 1983年)とされている。In matsutake shiro, hyphae penetrate between cells from the root epidermis of red pine, while they spread into the soil to absorb soil moisture and nutrients and send them to the pine, receiving sugar and vitamins in return ( "How to Make Matsutake Mt." P3 Matsutake Research Council, edited by Sobun 1983).
しかし本発明者は、特許出願公開昭53-2254(発明者
富永保人)「マツタケのトンネル方式栽培法」を昭和
53年以降10年以上にわたって試み、マツタケシロの上面
に人工かん水を実施した結果菌根の集合体であるシロは
極めて透水性が悪いことを知った。However, the present inventor has disclosed a patent application publication 53-2254 (inventor Yasuto Tominaga) "Matsutake Tunnel Cultivation Method" in Showa
Attempted for more than 10 years since 53 years, and artificial watering on the top of Matsutake Shiro found that Shiro, an aggregate of mycorrhiza, had extremely poor permeability.
そこで、種菌苗の枯損の主な原因は、それを植栽した
林内の日照不足だけでなく、菌根の不透水性、すなわち
撥水性が強いため、シロ内が乾燥して養水分の吸収が妨
げられることであると考えるに至った。Therefore, the main cause of the death of the seed fungus is not only the lack of sunshine in the forest where it was planted, but also the imperviousness of the mycorrhiza, that is, the strong water repellency, the inside of the white is dried and the absorption of nutrient water is absorbed. I came to consider it to be hindered.
(課題を解決するための手段) 本発明では、前記説明の種菌苗の枯損を防ぐため、苗
木の根系を分割し、一方を菌根形成根系とし、他方を養
水分吸収専用根系とした。(Means for Solving the Problems) In the present invention, in order to prevent the seed fungi described above from dying, the root system of the seedling is divided, and one of the root systems is a mycorrhiza-forming root system and the other is a root system dedicated to absorbing nutrients and moisture.
すなわち、第1図により説明すると、寄主植物苗
(1)を隔離手段として2個のポット(4)(5)に根
系を(2)(3)に分割して植栽。一年間養成して根系
を発達させた後、一方のポット(4)内の根系(2)
に、マツタケ、シメジ等の菌根菌を接種して菌根を形成
させる。他方自然感染法をとる場合は寄主植物苗(1)
を前記同様隔離手段として2個のポットに根系を分割し
て一年間養成して、根系を発達させた後第2図のように
ポット(4)(5)をはずして苗(1)の根系(2)
(3)を露出し、一方の根系(2)をシロ(7)の進行
方向の先端に接触するように植栽して1年を経過、菌根
を形成させる。前記2方法いずれの場合も、菌根形成確
認後、第3図のように、寄主樹林内の適齢木(9)の二
次感染用の細根のあるところに移植し、二次感染を図
る。 この時、菌根形成根系(2)と養水分吸収根系
(3)の間に充分な間隙をあけるか、または根系の隔離
を確実にするため障壁(6)を設けるかいずれかの方法
で、菌根形成根系(2)の菌根菌が養水分吸収根系
(3)に感染しないようにする。That is, referring to FIG. 1, the root system is divided into two plants (2) and (3) and planted in two pots (4) and (5) using the host plant seedling (1) as an isolation means. After one year of training and root system development, the root system (2) in one pot (4)
Is then inoculated with mycorrhizal fungi such as matsutake and shimeji to form mycorrhiza. On the other hand, when taking the natural infection method, host plant seedlings (1)
In the same manner as above, the root system is divided into two pots and cultivated for one year. After the root system is developed, the pots (4) and (5) are removed as shown in FIG. (2)
(3) is exposed, and one root system (2) is planted so as to be in contact with the tip in the traveling direction of the white (7), and one year has passed to form mycorrhiza. In any of the above two methods, after confirmation of mycorrhizal formation, as shown in FIG. 3, the tree is transplanted to a suitable-aged tree (9) in a place where there is a fine root for secondary infection, and secondary infection is carried out. At this time, either a sufficient gap is provided between the mycorrhizal root system (2) and the nutrient-absorbing root system (3), or a barrier (6) is provided to ensure isolation of the root system. Prevent mycorrhizal fungi in the mycorrhizal root system (2) from infecting the nutrient and water absorbing root system (3).
障壁板(6)の大きさはポット(4)(5)の直径×
深さ程度の適宜でよく材質も菌の侵入を防止できればプ
ラスチック板等なんでもよい。The size of the barrier plate (6) is the diameter of the pots (4) and (5) x
Any material such as a plastic plate may be used as long as it can be set to an appropriate depth and the material can prevent the invasion of bacteria.
また第4図のように隔離手段として培土を入れた2個
の育成箱(10)を使用数本の寄主樹苗(1)を集団で植
栽し、根系を菌根形成根系(2)と養水分吸収根系
(3)に分割植栽することで(2)の根系の集団にシロ
を形成することができる。Also, as shown in Fig. 4, two growing boxes (10) containing soil are used as isolation means. Several host tree seedlings (1) are planted in groups, and the root system is cultivated as a mycorrhizal root system (2). By dividing and planting in the water-absorbing root system (3), whites can be formed in the root system group of (2).
次に第5図のように数本の寄主樹苗(1)を育成箱
(10)に培土を入れて植え、寄主樹(1)の直根(12)
を隔離手段として育成箱(10)の底にあけた穴から下に
出して、直下に受けた受皿(11)の中の水耕液、または
培土に根系を発達させて養水分吸収根系とし、育成箱
(10)中に発達した根系(2)に菌根菌を接種して菌根
形成根系としてここにシロを形成することができる。反
対に根系(2)を養水分吸収根系に、直根(12)の根系
を菌根菌接種根系とすることもできる。Next, as shown in Fig. 5, several host tree seedlings (1) are planted with cultivated soil in the breeding box (10), and the direct roots (12) of the host tree (1) are planted.
As a means of isolation, take out from the hole drilled at the bottom of the breeding box (10), and develop the root system in the hydroponic solution or cultivation in the saucer (11) received directly below, as a root system for absorbing nutrients and water, The root system (2) developed in the breeding box (10) can be inoculated with mycorrhizal fungi to form white here as a mycorrhizal root system. Conversely, the root system (2) may be used as a nutrient and water absorbing root system, and the root system (12) may be used as a root system inoculated with mycorrhizal fungi.
(実施例1) 初年度春3月〜4月に、2年生赤松苗の根部を滅菌剤
ベンレート500倍液に1時間浸漬して、滅菌をした後洗
浄、根系を2つに分けて2個の直径15cmのビニールポッ
トの内側に同径の網ポットを重ねた後培土を入れて植
え、支柱に固定して幹を支える。(Example 1) From March to April in the first year of spring, the roots of the two-year-old red pine seedlings were immersed in a 500-fold solution of a sterilizing agent Benrate for 1 hour, sterilized, washed, and divided into two root systems. After laying a net pot of the same diameter inside a plastic pot with a diameter of 15cm, put the cultivation and plant it, fix it to a support and support the trunk.
これを適宜かん水をしながら、9月まで養成する。
別に浜田水培地(水1000cc、ブドウ糖20g、エビオス5
g、PH5に調整)で1、2ヵ月間培養したマツタケ菌糸を
精製水で洗浄し前記の赤松苗の二分した根系の一方に
接種して、これを9月から翌年5月まで養成する。It is cultivated until September while watering it appropriately.
Separately, Hamada water culture medium (water 1000cc, glucose 20g, ebios 5
g, adjusted to PH5) and washed with purified water for 1 to 2 months, inoculated into one of the two divided root systems of the red pine seedling, and cultivated from September to the following May.
次に30年位の樹齢の赤松林内のマツタケ未発生の赤松
細根の多い場所を選んでのマツタケ菌接種苗を、ポッ
トをはずして植栽、この時、マツタケ菌接種根系と非接
種根系の中間に20cm×15cmの大きさのプラスチック板を
置いて、マツタケ菌接種根系から、非接種根系へのマツ
タケ菌の感染を防ぎながら、成木の赤松根系へのマツタ
ケ菌の感染を図る。Next, in a red pine forest about 30 years old, select a place with a lot of red pine fine roots where matsutake does not occur, plant the seedlings inoculated with matsutake fungi in the pot, and plant at this time. A 20cm x 15cm plastic plate is placed on the pine to prevent the infection of the matsutake fungi from the inoculum root system to the non-inoculated root system, while infecting the matsutake fungi on the adult red pine root system.
(実施例2) 初年度実施例1ののようにして育成した赤松2年苗
を次年度春、マツタケシロの前方に、根部を網ポットに
入れたまま、ビニールポットより取り出して、ふたつの
根系のうち一方をマツタケシロの先端に接触するように
して植栽する。(Example 2) In the spring of the next fiscal year, the two-year-old red pine seedlings grown as in Example 1 of the first year were taken out of the plastic pot in front of Matsutake Shiro, with the roots still in the net pot, and two root systems were obtained. One of them is planted in contact with the tip of Matsutake Shiro.
翌年春、この根系にマツタケシロの菌が伸びて赤松苗
の根系まで達して感染するのを確認後、網ポットごと掘
り取り、マツタケ未発生赤松林内の赤松細根の多い場所
に移植する。この時、実施例1ののようにマツタケ菌
感染根系と未感染根系の中間にプラスチックの20cm×15
cmの大きさの障壁板を置いて未感染根系へのマツタケ菌
の侵入を防ぎながら適齢寄主樹の根系への感染を図る。In the spring of the following year, after confirming that the fungus of matsutake shiro grows on the root system and reaches the root system of the red pine seedlings, it is digged together with a net pot and transplanted to a place where there are many red pine fine roots in the pine forest where no matsutake occurs. At this time, as in Example 1, a plastic 20 cm × 15 was placed between the root system infected with Matsutake fungus and the root system uninfected.
A barrier plate with a size of cm is placed to prevent the infestation of Matsutake fungi into the uninfected root system and to infect the root system of the appropriate-age host tree.
(実施例3) 実施例1の、のようにして養成した赤松種菌苗を
2個の育成箱(10)を連結して培土を入れて一方にマツ
タケ菌接種根系を植え、他方にはマツタケ菌非接種根系
を植え、これを5、6本連続して植栽する。(Example 3) Two seedlings (10) of the Akamatsu seed fungus grown in the same manner as in Example 1 were connected to each other, and cultivation was put in the seedlings. A non-inoculated root system is planted, and five or six of these are continuously planted.
非接種根系には施肥をして松苗を肥育して、間接的に
マツタケ菌接種根系を発達させ、シロを形成して子実体
を発生させる。The non-inoculated root system is fertilized to grow pine seedlings, indirectly develop the matsutake fungus inoculated root system, form whites, and generate fruiting bodies.
(実施例4) 初年度春、第5図のように、2年生赤松苗(1)を育
成箱(10)に培土を入れて、20cm間隔に数本植栽する。(Example 4) In the spring of the first year, as shown in Fig. 5, two-year-old red pine seedlings (1) are cultivated in a breeding box (10), and several are planted at intervals of 20 cm.
この時育成箱(10)の底に直径1〜2cmの穴をあけ
て、赤松苗(1)の直根(12)を、この穴から外に出
し、この直根の先端を、育成箱(10)の直下すれすれに
受けるようにして設置した受皿(11)に入れた窒素、リ
ン酸、カリのほか微量成分を含んだ水耕液に浸すように
して、1年間この赤松苗(1)を養成する。At this time, a hole having a diameter of 1 to 2 cm was made in the bottom of the growing box (10), and the straight root (12) of the red pine seedling (1) was taken out of the hole. The red pine seedlings (1) were immersed for one year in a hydroponic solution containing nitrogen, phosphoric acid, potassium, and other minor components in a saucer (11) placed just below 10). Train.
この間に赤松苗(1)の側根(2)は育成箱(10)の
中の培土中に発達し、菌根菌接種好適根系となり、赤松
苗(1)の直根(12)の先端は水耕液中に細根を発達さ
せ養水分吸収根系となる 次年度春、根系(2)に実施例1ののようにして育
成箱(10)の中の根系(2)に菌根菌を接種する。During this time, the lateral roots (2) of the red pine seedlings (1) develop in the cultivation soil in the breeding box (10) and become a suitable root system for mycorrhizal fungi inoculation. In the spring of next year, the root system (2) is inoculated with the mycorrhizal fungi in the root system (2) in the growing box (10) as in Example 1 in the spring of the following year. .
この後1年間、受皿(11)中の水耕液の濃度を適度に
調整、維持しながら、育成箱(10)の培土に必要量のか
ん水をして赤松苗(1)を養成すると、育成箱(10)の
中に根系(2)に接種した菌根菌が菌根を作り、シロが
形成される。After one year, while adjusting and maintaining the concentration of the hydroponic solution in the saucer (11) appropriately, watering the cultivation soil in the cultivation box (10) with the required amount of water to grow the red pine seedlings (1) Mycorrhizal fungi inoculated into the root system (2) in the box (10) form mycorrhiza, forming whites.
(発明の効果) 従来の寄主植物の苗の根系をひとつにした種菌苗の方
法では寄主樹成木林内に種菌苗を植栽して苗根菌の寄主
成木の細根に二次感染するのを待つ期間中に、種菌苗自
身の根系中の菌根菌感染域が拡大し、相対的に養水分吸
収域が減少するため水分や養分が吸収できにくくなり種
菌苗の枯死を招いた。(Effect of the Invention) According to the conventional method of seed fungus seedling in which the root system of the seedlings of the host plant is united, the seed fungus seedlings are planted in the host tree-grown tree forest, and the roots of the seedling fungus are secondary infected to the fine roots of the host tree. During the waiting period, the area of mycorrhizal fungal infection in the root system of the seed fungus itself expanded and the absorption area of nutrient water decreased, making it difficult to absorb water and nutrients, resulting in the death of the seed fungus.
しかし本発明によれば、寄主樹の根系を隔離すること
により養水分吸収根系への菌根菌の感染が妨げられて種
菌苗の枯損がなくなり、寄主樹成木の根系への二次感
染、すなわちシロの形成が達成される。However, according to the present invention, by isolating the root system of the host tree, the infection of the mycorrhizal fungi into the nutrient and water absorption root system is prevented, the seed fungus seedlings do not die, and the secondary infection of the root system of the host tree, that is, White formation is achieved.
また、従来菌根形成苗には雑菌の侵入を防ぐため無施
肥、無養分の培土が必要なため、苗の成育が貧弱であっ
たが、本発明によれば根系を隔離し菌根形成根系は無施
肥として、養水分吸収根系には施肥が可能となるため、
菌根菌接種苗は強健に成育するので、この寄主樹苗を集
団で植栽することにより、実施例(3)、(4)のよう
に育成箱の中に菌根菌のシロの形成が可能となる。In addition, conventional mycorrhizal seedlings require no fertilization and nourishment to prevent the invasion of various bacteria, so that the growth of the seedlings was poor.However, according to the present invention, the root system was isolated and the mycorrhizal root system was isolated. No fertilization, fertilization is possible for the nutrient absorption root system,
Since the mycorrhizal fungi-inoculated seedling grows vigorously, by planting this host tree seedling in a group, it is possible to form mycorrhizal fungi in the growing box as in Examples (3) and (4). Becomes
第1図は養成中の寄主植物苗の斜視図 第2図は自然シロを使って菌根菌を感染させるための寄
主植物苗の配置の説明図 第3図は種菌苗を寄主樹林内に植栽して二次感染を図る
斜視図 第4図は育成箱に種菌苗を植えてシロを形成させる実施
例を示す斜視図 第5図は種菌苗の側根を菌根菌接種根系とし、同じ種菌
苗の直根を水耕液中に発達させて養分吸収根系としたシ
ロ形成実施例の説明図。 1:寄主植物苗、2:菌根菌接種根系 3:菌根菌非接種根系、4:ポット 5:ポット、6:障壁板 7:自然シロ、8:寄主樹成木 9:未感染寄主樹成木、10:育成箱 11:水耕液受皿、12:寄主植物苗の直根Fig. 1 is a perspective view of a host plant seedling during training. Fig. 2 is an explanatory view of the arrangement of a host plant seedling for infecting mycorrhizal fungi using natural white. Fig. 3 is planting seed fungus seedlings in a host tree forest. Fig. 4 is a perspective view showing an embodiment in which a seed fungus is planted in a growth box to form a white spot. Fig. 5 is a side view of the seed fungus seedling as a mycorrhizal fungus inoculating root system. Explanatory drawing of the white forming example which developed the direct root of a seedling in a hydroponic solution and made it a nutrient absorption root system. 1: Host plant seedling, 2: Mycorrhizal fungi inoculated root system 3: Non-mycorrhizal fungal inoculated root system, 4: Pot 5: Pot, 6: Barrier plate 7: Natural white, 8: Host tree 9: Uninfected host tree Adult tree, 10: Cultivation box 11: Hydroponic liquid receiving tray, 12: Direct root of host plant seedling
Claims (5)
し、一部の根系に菌根菌を接種して菌根を形成させ、残
余の根系を養水分吸収根系とする種菌苗を使用すること
を特徴とする菌根菌のシロ形成方法。1. The root system of a host tree is divided through an isolation means, a part of the root system is inoculated with mycorrhizal fungi to form mycorrhiza, and the seed fungus seedling which uses the remaining root system as a nutrient and water absorbing root system. A method for forming whites of mycorrhizal fungi, which is used.
トに寄主樹の苗木の根系を分割して植え、一方のポット
内の根系に菌根菌を接種して菌根を形成させ、他方のポ
ット内の根系を養水分吸収根系とする種菌苗を、寄主樹
林内に植栽して、菌根菌の二次感染を図りシロを形成さ
せる特許請求の範囲第1項記載の菌根菌のシロ形成方
法。2. As an isolation means, a root system of a seedling of a host tree is divided and planted in two pots containing soil, and the root system in one pot is inoculated with mycorrhizal fungi to form mycorrhizas. The mycorrhiza according to claim 1, wherein a seed fungus seedling having the root system in the other pot as a nutrient and water-absorbing root system is planted in a host tree forest to achieve secondary infection of mycorrhizal fungi and form whites. A method for forming fungi.
箱を使用、少なくとも1本の寄主樹の苗木の根系を分割
してそれぞれに植え、一方の育成箱内の根系に菌根菌を
接種して菌根を形成させ、他方の育成箱内の根系を養水
分吸収根系とする特許請求の範囲第1項記載の菌根菌の
シロ形成方法。3. A method for isolating two cultivation boxes containing cultivated soil, dividing and cultivating at least one root tree seedling root system, and arranging mycorrhizal fungi in one of the cultivation boxes. 2. The method of claim 1, wherein the root system in the other growing box is used as a nutrient and water-absorbing root system.
直根に発達した根系とを隔離して、そのいずれか一方を
養水分吸収根系とし、他方を菌根形成根系とする種菌苗
を使用することを特徴とする特許請求の範囲第1項記載
の菌根菌のシロ形成方法。4. The host system according to claim 1, wherein the root system is a root system developed in lateral roots.
2. The method according to claim 1, wherein the root system developed in the direct root is isolated, and a seed fungus seedling having one of the root system as a nutrient / water absorbing root system and the other as a mycorrhizal root system is used. A method for forming mycorrhizal whites.
寄主樹の苗を植え、隔離手段として、この寄主樹の苗の
直根を育成箱下部にあけた穴を通して、下に設置した水
耕液または培土を入れた受皿にたらして根系を形成さ
せ、育成箱の培土の中に発達した根系とのいずれか一方
を養水分吸収根系とし、他方を菌根形成根系とする特許
請求の範囲第4項記載の菌根菌のシロ形成方法。5. A seedling of at least one host tree is planted in a breeding box containing soil, and as a means of isolation, a direct root of the seedling of the host tree is placed below through a hole drilled at the bottom of the breeding box. Claims that the root system is formed by placing it on a saucer containing a hydroponic solution or soil, and one of the root system developed in the soil of the growing box as a nutrient-absorbing root system and the other as a mycorrhizal root system. 5. The method for forming white mold of mycorrhizal fungi according to claim 4.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2231062A JP2866983B2 (en) | 1990-08-31 | 1990-08-31 | Method for mycorrhizal fungus whitening |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2231062A JP2866983B2 (en) | 1990-08-31 | 1990-08-31 | Method for mycorrhizal fungus whitening |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH04112724A JPH04112724A (en) | 1992-04-14 |
JP2866983B2 true JP2866983B2 (en) | 1999-03-08 |
Family
ID=16917691
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2231062A Expired - Lifetime JP2866983B2 (en) | 1990-08-31 | 1990-08-31 | Method for mycorrhizal fungus whitening |
Country Status (1)
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JP (1) | JP2866983B2 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6907691B2 (en) * | 2002-06-26 | 2005-06-21 | Stewart C. Miller | Cultivation of morchella |
JP5612340B2 (en) * | 2010-03-19 | 2014-10-22 | 国立大学法人鳥取大学 | Novel method of mycorrhiza formation |
CN106718717B (en) * | 2016-11-17 | 2020-10-09 | 华侨大学 | Cultivation method for improving quality of turmeric medicinal material |
JP6845541B2 (en) * | 2017-07-07 | 2021-03-17 | 地方独立行政法人北海道立総合研究機構 | How to make Matsutake mushroom root seedlings |
CN115316252B (en) * | 2022-10-10 | 2023-01-31 | 中国科学院昆明植物研究所 | Method for establishing root division system in herbaceous root hemiparasitic plant vessel |
-
1990
- 1990-08-31 JP JP2231062A patent/JP2866983B2/en not_active Expired - Lifetime
Also Published As
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JPH04112724A (en) | 1992-04-14 |
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