JP2024530711A - 体積撮像 - Google Patents

体積撮像 Download PDF

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Publication number
JP2024530711A
JP2024530711A JP2024510338A JP2024510338A JP2024530711A JP 2024530711 A JP2024530711 A JP 2024530711A JP 2024510338 A JP2024510338 A JP 2024510338A JP 2024510338 A JP2024510338 A JP 2024510338A JP 2024530711 A JP2024530711 A JP 2024530711A
Authority
JP
Japan
Prior art keywords
light
pixels
image sensor
sample
sections
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2024510338A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2023021289A5 (https=
JP2024530711A5 (https=
Inventor
シュトゥロール、フロリアン
Original Assignee
ウニベルシテテット イ トロムソ－ノルゲス アークティスク ウニベルシテット
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB2111782.5A external-priority patent/GB202111782D0/en
Priority claimed from GBGB2206966.0A external-priority patent/GB202206966D0/en
Application filed by ウニベルシテテット イ トロムソ－ノルゲス アークティスク ウニベルシテット filed Critical ウニベルシテテット イ トロムソ－ノルゲス アークティスク ウニベルシテット
Publication of JP2024530711A publication Critical patent/JP2024530711A/ja
Publication of JPWO2023021289A5 publication Critical patent/JPWO2023021289A5/ja
Publication of JP2024530711A5 publication Critical patent/JP2024530711A5/ja
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/006—Optical details of the image generation focusing arrangements; selection of the plane to be imaged
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/1066—Beam splitting or combining systems for enhancing image performance, like resolution, pixel numbers, dual magnifications or dynamic range, by tiling, slicing or overlapping fields of view
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/178—Methods for obtaining spatial resolution of the property being measured
    • G01N2021/1785—Three dimensional
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6467—Axial flow and illumination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/064—Stray light conditioning
    • G01N2201/0648—Shutters

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Length Measuring Devices By Optical Means (AREA)
JP2024510338A 2021-08-17 2022-08-17 体積撮像 Pending JP2024530711A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB2111782.5A GB202111782D0 (en) 2021-08-17 2021-08-17 Volumetric imaging
GB2111782.5 2021-08-17
GBGB2206966.0A GB202206966D0 (en) 2022-05-12 2022-05-12 Volumetric imaging
GB2206966.0 2022-05-12
PCT/GB2022/052135 WO2023021289A1 (en) 2021-08-17 2022-08-17 Volumetric imaging

Publications (3)

Publication Number Publication Date
JP2024530711A true JP2024530711A (ja) 2024-08-23
JPWO2023021289A5 JPWO2023021289A5 (https=) 2025-08-13
JP2024530711A5 JP2024530711A5 (https=) 2025-08-13

Family

ID=83193236

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2024510338A Pending JP2024530711A (ja) 2021-08-17 2022-08-17 体積撮像

Country Status (4)

Country Link
US (1) US20240418652A1 (https=)
EP (1) EP4388362A1 (https=)
JP (1) JP2024530711A (https=)
WO (1) WO2023021289A1 (https=)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013106731A1 (en) * 2012-01-11 2013-07-18 Howard Hughes Medical Institute Multi-dimensional imaging using multi-focus microscopy
US9823457B2 (en) * 2014-01-08 2017-11-21 The Regents Of The University Of California Multiplane optical microscope
US10409052B2 (en) * 2016-09-28 2019-09-10 University Of Washington Inverted light-sheet microscope
WO2019118582A2 (en) * 2017-12-12 2019-06-20 Trustees Of Boston University Multi-z confocal imaging system
US11231570B2 (en) * 2018-04-30 2022-01-25 University Of Central Florida Research Foundation, Inc. Highly inclined swept tile (HIST) imaging apparatus, methods, and applications
DE102020209889A1 (de) * 2020-08-05 2022-02-10 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren zur mikroskopischen Bildaufnahme mit variabler Beleuchtung

Also Published As

Publication number Publication date
EP4388362A1 (en) 2024-06-26
WO2023021289A1 (en) 2023-02-23
US20240418652A1 (en) 2024-12-19

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