JP2023540934A - Ｒａｓタンパク質のターゲットエンゲージメントアッセイ - Google Patents
Ｒａｓタンパク質のターゲットエンゲージメントアッセイ Download PDFInfo
- Publication number
- JP2023540934A JP2023540934A JP2023514021A JP2023514021A JP2023540934A JP 2023540934 A JP2023540934 A JP 2023540934A JP 2023514021 A JP2023514021 A JP 2023514021A JP 2023514021 A JP2023514021 A JP 2023514021A JP 2023540934 A JP2023540934 A JP 2023540934A
- Authority
- JP
- Japan
- Prior art keywords
- ras
- kras
- methyl
- protein
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063071694P | 2020-08-28 | 2020-08-28 | |
| US63/071,694 | 2020-08-28 | ||
| US202063117080P | 2020-11-23 | 2020-11-23 | |
| US63/117,080 | 2020-11-23 | ||
| US202163160120P | 2021-03-12 | 2021-03-12 | |
| US63/160,120 | 2021-03-12 | ||
| PCT/US2021/047998 WO2022047186A1 (en) | 2020-08-28 | 2021-08-27 | Target engagement assay for ras proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2023540934A true JP2023540934A (ja) | 2023-09-27 |
| JPWO2022047186A5 JPWO2022047186A5 (https=) | 2024-09-03 |
| JP2023540934A5 JP2023540934A5 (https=) | 2024-09-03 |
Family
ID=77914456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023514021A Pending JP2023540934A (ja) | 2020-08-28 | 2021-08-27 | Ｒａｓタンパク質のターゲットエンゲージメントアッセイ |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20220064696A1 (https=) |
| EP (1) | EP4204808A1 (https=) |
| JP (1) | JP2023540934A (https=) |
| CN (1) | CN116391129A (https=) |
| WO (1) | WO2022047186A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20210006356A (ko) | 2018-04-04 | 2021-01-18 | 아비나스 오퍼레이션스, 인코포레이티드 | 단백질분해 조절제 및 연관된 사용 방법 |
| CN121419983A (zh) | 2023-01-26 | 2026-01-27 | 阿尔维纳斯运营股份有限公司 | 基于小脑蛋白的kras降解protac及其相关用途 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016500263A (ja) * | 2012-12-12 | 2016-01-12 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4812409A (en) | 1986-01-31 | 1989-03-14 | Eastman Kodak Company | Hydrolyzable fluorescent substrates and analytical determinations using same |
| US4810636A (en) | 1986-12-09 | 1989-03-07 | Miles Inc. | Chromogenic acridinone enzyme substrates |
| US6162931A (en) | 1996-04-12 | 2000-12-19 | Molecular Probes, Inc. | Fluorinated xanthene derivatives |
| US7429472B2 (en) | 2003-01-31 | 2008-09-30 | Promega Corporation | Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule |
| EP1594962B1 (en) | 2003-01-31 | 2014-08-27 | Promega Corporation | Covalent tethering of functional groups to proteins |
| US7425436B2 (en) | 2004-07-30 | 2008-09-16 | Promega Corporation | Covalent tethering of functional groups to proteins and substrates therefor |
| WO2008118445A1 (en) | 2007-03-26 | 2008-10-02 | Promega Corporation | Methods to quench light from optical reactions |
| GB0905840D0 (en) | 2009-04-06 | 2009-05-20 | Sagentia Ltd | Apparatus and methods |
| ES2795287T3 (es) | 2009-05-01 | 2020-11-23 | Promega Corp | Luciferasas de Oplophorus sintéticas con mayor emisión de luz |
| JP5677041B2 (ja) | 2009-11-11 | 2015-02-25 | 株式会社ニデック | 眼科装置 |
| SG10202103336SA (en) | 2010-11-02 | 2021-04-29 | Promega Corp | Novel coelenterazine substrates and methods of use |
| GB201103631D0 (en) * | 2011-03-03 | 2011-04-13 | Univ Leeds | Identification of candidate therapeutics |
| JP5751030B2 (ja) | 2011-06-03 | 2015-07-22 | ソニー株式会社 | 表示制御装置、表示制御方法、及びプログラム |
| WO2013078244A1 (en) | 2011-11-21 | 2013-05-30 | Promega Corporation | Carboxy x rhodamine analogs |
| US20140099654A1 (en) | 2012-09-26 | 2014-04-10 | Promega Corporation | Real-time monitoring |
| US10067149B2 (en) * | 2012-12-12 | 2018-09-04 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
| ES2926463T3 (es) | 2013-03-15 | 2022-10-26 | Promega Corp | Activación de la bioluminiscencia mediante complementación estructural |
| JP2018533939A (ja) * | 2015-10-19 | 2018-11-22 | アラクセス ファーマ エルエルシー | Ｒａｓの阻害剤をスクリーニングするための方法 |
| JP7092677B2 (ja) | 2016-02-15 | 2022-06-28 | プロメガ コーポレイション | セレンテラジン類似体 |
| JP7212612B2 (ja) | 2016-07-28 | 2023-01-25 | プロメガ コーポレイション | セレンテラジン類縁体 |
| WO2019173683A1 (en) * | 2018-03-08 | 2019-09-12 | Stc. Unm | Discovery of novel molecules and repurposed drugs for ras family gtpases |
| WO2019241438A2 (en) | 2018-06-12 | 2019-12-19 | Promega Corporation | Multipartite luciferase |
-
2021
- 2021-08-27 EP EP21777898.4A patent/EP4204808A1/en active Pending
- 2021-08-27 WO PCT/US2021/047998 patent/WO2022047186A1/en not_active Ceased
- 2021-08-27 US US17/459,502 patent/US20220064696A1/en active Pending
- 2021-08-27 JP JP2023514021A patent/JP2023540934A/ja active Pending
- 2021-08-27 CN CN202180074299.3A patent/CN116391129A/zh active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016500263A (ja) * | 2012-12-12 | 2016-01-12 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
Non-Patent Citations (3)
| Title |
|---|
| ACS CHEM. BIOL., vol. 11, JPN6025034983, 2016, pages 400 - 408, ISSN: 0005772342 * |
| METHODS MOL BIOL., vol. 1888, JPN6025034982, 2019, pages 45 - 71, ISSN: 0005772344 * |
| PNAS, vol. 116(32), JPN6025034981, 2019, pages 15823 - 15829, ISSN: 0005772343 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20220064696A1 (en) | 2022-03-03 |
| EP4204808A1 (en) | 2023-07-05 |
| WO2022047186A1 (en) | 2022-03-03 |
| CN116391129A (zh) | 2023-07-04 |
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