JP2018522565A5 - - Google Patents
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- JP2018522565A5 JP2018522565A5 JP2018506219A JP2018506219A JP2018522565A5 JP 2018522565 A5 JP2018522565 A5 JP 2018522565A5 JP 2018506219 A JP2018506219 A JP 2018506219A JP 2018506219 A JP2018506219 A JP 2018506219A JP 2018522565 A5 JP2018522565 A5 JP 2018522565A5
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- Japan
- Prior art keywords
- seq
- promoter
- nucleotide sequence
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- nos
- Prior art date
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- 239000002773 nucleotide Substances 0.000 claims description 48
- 125000003729 nucleotide group Chemical group 0.000 claims description 48
- 230000001105 regulatory effect Effects 0.000 claims description 44
- 108091023040 Transcription factor Proteins 0.000 claims description 25
- 102000040945 Transcription factor Human genes 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 23
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 22
- 210000004027 cell Anatomy 0.000 claims description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 13
- 229910052799 carbon Inorganic materials 0.000 claims description 13
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
- 102000039446 nucleic acids Human genes 0.000 claims description 9
- 108020004707 nucleic acids Proteins 0.000 claims description 9
- 150000007523 nucleic acids Chemical class 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 230000000153 supplemental effect Effects 0.000 claims description 6
- 238000012217 deletion Methods 0.000 claims description 5
- 230000037430 deletion Effects 0.000 claims description 5
- 238000011144 upstream manufacturing Methods 0.000 claims description 5
- 101100099277 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) rgt-1 gene Proteins 0.000 claims description 4
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 4
- 230000006698 induction Effects 0.000 claims description 4
- 238000013518 transcription Methods 0.000 claims description 4
- 230000035897 transcription Effects 0.000 claims description 4
- 210000005253 yeast cell Anatomy 0.000 claims description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 3
- 241000235648 Pichia Species 0.000 claims description 3
- 241000235070 Saccharomyces Species 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 230000014621 translational initiation Effects 0.000 claims description 3
- 230000000295 complement effect Effects 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 230000002538 fungal effect Effects 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 2
- 239000011701 zinc Substances 0.000 claims 2
- 229910052725 zinc Inorganic materials 0.000 claims 2
- 102000018779 Replication Protein C Human genes 0.000 claims 1
- 108010027647 Replication Protein C Proteins 0.000 claims 1
- 239000012190 activator Substances 0.000 claims 1
- 230000006377 glucose transport Effects 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 229940113082 thymine Drugs 0.000 claims 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 241000235058 Komagataella pastoris Species 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 239000012526 feed medium Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- -1 sucrose Chemical class 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 241001099157 Komagataella Species 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP2015068024 | 2015-08-05 | ||
| EPPCT/EP2015/068024 | 2015-08-05 | ||
| EP16163932 | 2016-04-05 | ||
| EP16163932.3 | 2016-04-05 | ||
| PCT/EP2016/068784 WO2017021541A1 (en) | 2015-08-05 | 2016-08-05 | Promoter variants |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018522565A JP2018522565A (ja) | 2018-08-16 |
| JP2018522565A5 true JP2018522565A5 (https=) | 2020-08-06 |
| JP6833812B2 JP6833812B2 (ja) | 2021-02-24 |
Family
ID=56684642
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018506219A Active JP6833812B2 (ja) | 2015-08-05 | 2016-08-05 | プロモーター変異体 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US10752907B2 (https=) |
| EP (1) | EP3332005B1 (https=) |
| JP (1) | JP6833812B2 (https=) |
| KR (1) | KR102253479B1 (https=) |
| CN (1) | CN108350447B (https=) |
| AU (1) | AU2016303026B2 (https=) |
| BR (1) | BR112018002395A2 (https=) |
| CA (1) | CA2992170C (https=) |
| DK (1) | DK3332005T3 (https=) |
| ES (1) | ES2902044T3 (https=) |
| IL (1) | IL257000B (https=) |
| TW (1) | TW201718859A (https=) |
| WO (1) | WO2017021541A1 (https=) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI560272B (en) | 2011-10-07 | 2016-12-01 | Lonza Ag | Regulatable promoter |
| JP7717459B2 (ja) | 2018-02-12 | 2025-08-04 | ロンザ リミテッド | 目的タンパク質を産生するための宿主細胞 |
| EP3536784A1 (en) | 2018-03-05 | 2019-09-11 | ACIB GmbH | Host cell engineered for improved metabolite production |
| EP3802832A1 (en) * | 2018-06-07 | 2021-04-14 | Basf Se | Promoter for yeast |
| EP3824083A4 (en) * | 2018-09-24 | 2022-01-05 | Orta Dogu Teknik Universitesi | Design of alcohol dehydrogenase 2 (adh2) promoter variants by promoter engineering |
| JP2022502018A (ja) * | 2018-09-24 | 2022-01-11 | オルタ ドグ テクニク ユニヴェルシテシ | 改変されたａｏｘ１プロモータバリアント |
| DK3874047T4 (da) * | 2019-01-11 | 2025-02-03 | Lonza Ag | Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle |
| RS64248B1 (sr) * | 2019-01-29 | 2023-06-30 | Clariant Produkte Deutschland Gmbh | Himerni promotor za upotrebu u metaboličkom inženjeringu |
| AU2020252241B2 (en) | 2019-04-01 | 2024-03-21 | Universität Für Bodenkultur Wien | Mut- methylotrophic yeast |
| WO2020200414A1 (en) | 2019-04-01 | 2020-10-08 | Lonza Ltd | Protein production in mut-methylotrophic yeast |
| WO2020200415A1 (en) | 2019-04-01 | 2020-10-08 | Lonza Ltd | Mut- methylotrophic yeast |
| US11618900B2 (en) * | 2019-11-01 | 2023-04-04 | The Johns Hopkins University | Modulating SYNGAP |
| EP4127185B1 (en) | 2020-04-01 | 2025-06-04 | Lonza Ltd. | Helper factors for expressing proteins in yeast |
| CA3174666A1 (en) * | 2020-04-09 | 2021-10-14 | Robert Schlegl | Improved methods of producing a lipidated protein |
| CA3193155A1 (en) | 2020-09-30 | 2022-04-07 | Lonza Ltd | Host cells overexpressing translational factors |
| IL304910A (en) | 2021-02-12 | 2023-10-01 | Boehringer Ingelheim Rcv Gmbh | Signal peptides for increased protein secretion |
| CN114657190B (zh) * | 2022-04-06 | 2023-08-29 | 暨南大学 | Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用 |
| CN114908092B (zh) * | 2022-05-12 | 2023-07-18 | 华南理工大学 | 一种长效、高强度的杂合启动子及其重组菌株和应用 |
| EP4353825A1 (en) * | 2022-10-10 | 2024-04-17 | bisy GmbH | Modified promoter sequences |
| WO2024133538A1 (en) | 2022-12-20 | 2024-06-27 | Lonza Ltd | Host cells with pat1 knockout for increased specific protein productivity |
| EP4726051A1 (en) * | 2023-06-06 | 2026-04-15 | FUJIFILM Corporation | Recombinant yeast, method for producing target protein, and kit and method for producing recombinant yeast |
| CN121358867A (zh) * | 2023-06-15 | 2026-01-16 | 丹斯塔发酵股份公司 | C2碳源响应性启动子 |
| WO2025247966A1 (en) | 2024-05-29 | 2025-12-04 | Mybios Gmbh | Process for protein production in methylotrophic yeast host cell. |
| WO2026027251A1 (en) | 2024-08-01 | 2026-02-05 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Signal peptides for increased protein secretion |
| BE1032887B1 (nl) * | 2024-08-26 | 2026-04-14 | Anheuser Busch Inbev Sa | Geoptimaliseerd promotorontwerp voor industriëe toepassing |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT501955B1 (de) | 2005-02-23 | 2007-08-15 | Univ Graz Tech | Mutierte aox1-promotoren |
| US8105811B2 (en) | 2008-06-13 | 2012-01-31 | Wisconsin Alumni Research Foundation | Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use |
| EP2494053A4 (en) * | 2009-10-30 | 2013-10-30 | Merck Sharp & Dohme | PROCESS FOR PRODUCING RECOMBINANT PROTEINS WITH INCREASED SECRETION EFFICIENCY |
| TWI560272B (en) * | 2011-10-07 | 2016-12-01 | Lonza Ag | Regulatable promoter |
| AU2013341049B2 (en) | 2012-10-29 | 2017-07-20 | Lonza Ltd | Expression sequences |
-
2016
- 2016-08-05 DK DK16751266.4T patent/DK3332005T3/da active
- 2016-08-05 AU AU2016303026A patent/AU2016303026B2/en active Active
- 2016-08-05 KR KR1020187006217A patent/KR102253479B1/ko active Active
- 2016-08-05 WO PCT/EP2016/068784 patent/WO2017021541A1/en not_active Ceased
- 2016-08-05 BR BR112018002395A patent/BR112018002395A2/pt not_active IP Right Cessation
- 2016-08-05 JP JP2018506219A patent/JP6833812B2/ja active Active
- 2016-08-05 ES ES16751266T patent/ES2902044T3/es active Active
- 2016-08-05 TW TW105125044A patent/TW201718859A/zh unknown
- 2016-08-05 US US15/750,334 patent/US10752907B2/en active Active
- 2016-08-05 EP EP16751266.4A patent/EP3332005B1/en active Active
- 2016-08-05 CA CA2992170A patent/CA2992170C/en active Active
- 2016-08-05 CN CN201680058388.8A patent/CN108350447B/zh active Active
-
2018
- 2018-01-18 IL IL257000A patent/IL257000B/en unknown
-
2020
- 2020-06-29 US US16/914,866 patent/US12522837B2/en active Active
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