JP2017072490A - Transparent sheet with dummy ovum protrusion and observation method using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a transparent sheet with a dummy ovum protrusion to be coated with semen, which is a tool used in observation through a simplified microscope.SOLUTION: A transparent sheet with a dummy ovum protrusion is exclusively for use with a simplified microscope comprising a single objective lens configured to be brought into contact with one surface of the transparent sheet for observation, and is provided with a protrusion formed on the other surface that does not come into contact with the lens and designed to act as an obstruction to sperms. Provision of the transparent sheet with a dummy ovum protrusion to be coated with semen for use with a simplified microscope comprising a single objective lens allows for personally viewing sperms penetrating into the dummy ovum using a digital camera, such as a portable terminal device, and deriving sperm concentration using the same.SELECTED DRAWING: Figure 2

Description

The present invention relates to a transparent sheet for applying semen for observing the state of sperm with a simple microscope and an observation method using the same.

The present inventor manufactures and sells a simple microscope that observes sperm by applying semen to a transparent sheet, but the sperm misinterprets the sperm as an egg in Non-Patent Document 1 below and attempts to fertilize As a result, it was considered whether air bubbles could be fixed to the transparent sheet. However, it is very difficult to generate a bubble instead of an egg with the desired size and fix it at a constant position on the transparent sheet, so use a solid material instead of the gas that forms the bubble. Thus, the present application was completed, and the present application was determined to be patentable with respect to a series of sperm count methods for calculating sperm concentration using the present invention.

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The problem underlying the present invention is an opportunity to consult with a specialist at an early stage in order to eliminate the fact that infertility treatment, which is essential for early detection due to shame, delays infertility treatment and makes it impossible to make children. It is a thing. For that purpose, it is better for an individual to recognize the possibility of infertility to some extent by himself. Therefore, I thought it would be very good if I could observe the state of my sperm entering the bubbles with a simple microscope. In view of this, the first object of the present invention is to provide a transparent sheet with pseudo-oval projections on which semen, which is an instrument used for observation with a simple microscope, is applied.
In addition, when calculating the sperm concentration used for conventional indicators of infertility, the sperm concentration is obtained using its own simple microscope and a digital camera such as a portable terminal (hereinafter referred to as a digital camera such as a portable terminal). For this purpose, when counting the number of sperm, the objective lens part of the simple microscope is attached to the opening of the camera lens, but the attachment distance between the objective lens of the microscope and the lens of a digital camera such as a portable terminal Magnification and field of view differ depending on the difference in type and model, and the size of the field of view image displayed on the monitor is not constant. Therefore, even if the number of spermatozoa is counted in a round field of view frame on the monitor, the sperm count obtained from digital cameras such as mobile terminals owned by each person is not general, so it is compared with other data. There was no objectivity. Therefore, it is the second object of the present invention to solve the problem of generalization of the count number of sperm.

The simple microscope used for solving the problems of the present invention is a so-called Leeuwen hook (Lowen hook) type high-magnification object with a very short focal length, and the thickness of the spermatozoa observed by the microscope. The objective lens is brought into contact with one side of a thin transparent sheet, and the semen applied to the opposite side of the surface is observed. The transparent sheet forms a part of the transparent bag, and the sample is placed in the bag. In some cases, an objective lens is brought into contact with the outside and observed.

The transparent sheet with pseudo-egg projections of the present invention provided in the first problem is a product in which a projection portion that prevents the movement of sperm is provided on one side of a synthetic resin-like thin transparent sheet. The material of the protrusion may be soft or hard as long as it is not toxic to sperm. The shape of the protrusion is convenient if the cross-sectional shape of the protrusion is circular or square. For production, the female mold of this shape may be manufactured by integral molding, or spherical beads or synthetic resin sheets may be cut into a circle or square on the adhesive side of the transparent adhesive tape and adhesively fixed. A plurality of projections may be provided. Furthermore, since the transparent sheet with pseudo ovum protrusion is thin and soft, it is convenient to use a structure that is attached to a holding plate having a firm thickness.

Apply the semen to the protrusion side of the transparent sheet with pseudo-egg protrusions made in this way, and bring the objective lens of the simple microscope into contact with the opposite side to see the appearance of sperm in the semen applied to the protrusion side. Observe and record the sperm with a digital camera such as a portable terminal.
Thus, when observed, sperm can be observed as a sperm that mistakes for an egg and tries to enter the protrusion. Some instantaneously transform the head into a triangle. Such sperm can be seen as being highly fertilized sperm, but if you have no such sperm and you are weak or dead, you will surely think this is bad. Anyone who really wants to have a child will want to consult with a specialist at an early stage, and the problem underlying the present invention can be achieved to some extent, if not 100%.
The size of the ovum is a sphere having a diameter of about 0.1 mm. However, the shape (transverse cross-sectional shape) of the projection of the pseudo ovum according to the present invention is about 0.05 to It can be round or square of about 0.1 mm (depending on the magnification of the objective lens of the simple microscope). Once the optimum size is determined, it can be manufactured in the same size and shape, and the number of protrusions By unifying with the individual, the total number of spermatozoa per unit time to enter the pseudo-oval process can be used as an index value of fertilization ability.

In addition, in order to obtain the sperm concentration, which is one index for judging fertilization ability, by using a digital camera such as a simple microscope and a portable terminal and the transparent sheet with pseudo ovulation of the present invention, the sperm count number can be calculated. Can be generalized. As described in the section of the problem to be solved by the invention, the focal point in the round field frame displayed on the monitor screen depends on the mounting distance to the lens part of the digital camera such as a simple microscope and a portable terminal and the model. The number of spermatozoa has no generality, but the method of making it exist is the following idea of the present invention.

Among the transparent sheets with pseudo-oval projections, even if the area is unknown (it is better if it is known), the shape that is always constant is regarded as a gauge for setting magnification. For example, the projection of the transparent sheet with pseudo ovum of the present invention is regarded as a gauge, and is displayed on a monitor screen of a digital camera such as a portable terminal through a simple microscope, and the size of the image is forcibly defined as a predetermined area. The overall magnification of a digital camera such as a simple microscope and a portable terminal is determined by matching with the shape of a template with a fixed shape, and then a large hole template with a predetermined area of that is used to create a sperm. This is a method for generalizing the number of sperms by determining the count range and counting only the sperm at the inner focal point.
If a relational expression between the number of spermatozoa obtained in this way and the sperm concentration in the sperm motility analysis system CASA based on the WHO guidelines is obtained from a lot of data, the degree of infertility can be determined to some extent by individuals using the method of the present invention. You will be able to judge.

Specifically, an objective lens portion of a simple microscope for observation is attached and fixed to an opening of a digital camera such as a portable terminal so that the lens image is at an appropriate position on the screen of the portable terminal. Next, apply semen to the transparent sheet with pseudo ovulation (attached to the holding plate) and attach it to the objective lens part of the simple microscope, and display an image of the pseudo ovation on the monitor screen, Place the small hole of the template on the monitor screen, bring the hole on the image of the pseudo ovulation, and enlarge or reduce the figure magnification so that the image of the pseudo ovation fits in the hole. Take a picture and record it, and keep it unchanged. By doing this, the magnification of the image of a digital camera such as a portable terminal is the same even if the distance between the lenses when the simple microscope is attached or the model is different.
In addition, if the image of the pseudo ovum projection is circular or square, the diameter or the length of one side of the image is measured with a commercially available ruler so that the default length is 20 mm if it is for example 20 mm. The image is recorded by zooming in or out, and is not changed thereafter, so that the magnification can be made constant without using the graphic template regardless of the mounting distance or the model.
Next, move the position of the transparent sheet with pseudo ovulations to which semen is applied, or apply semen to another transparent sheet without pseudo ovulations, and only the semen containing the sperm of the part where there is no image of the pseudo ovation part Shoot and record the visible range. Place a template with a large hole on the image of the semen recorded in this way on the monitor screen and count only the focused sperm inside the hole. This is to limit the range of counting sperm. In this way, it is possible to obtain a generalized sperm count number that does not depend on the mounting distance of a simple microscope attached to a digital camera such as a portable terminal or the camera model, and the second problem of the present invention is Solvable. Here again, if it is not desired to use a large hole in the graphic template, a similar graphic having a predetermined multiple of the predetermined length may be drawn and pasted with graphics software.

In addition, when analyzing the information of the camera image in a personal computer, it is an image including a pseudo-oval projection that has been pre-scaled and recorded by the above method, and has a round or square shape that fits the pseudo-oval projection image. Draw a figure with graphics software and paste it, then draw a large hole figure with the specified ratio of the area of the large and small holes of the figure, and record it by the above method If you paste it on an image of only the semen part of a computer and count the sperm only in that figure, you can get a general numerical value based on the same idea as the above template and camera monitor screen. There is no change in the shape of the template, whether it is the operation of the actual template or the operation of the actual template. The idea of the invention in the screen of Tarukamera is the same thing for.

Therefore, in the method for solving the second problem of the present invention, the pseudo ovum projection of the transparent sheet with a pseudo ovum projection is regarded as a magnification determining gauge, and the size on the monitor screen of the camera is changed to a figure of a predetermined area. In this method, the total magnification of a simple microscope and a digital camera such as a smartphone is made constant, and the number of sperm in a figure larger than the figure of the predetermined area is counted to generalize the sperm count.

Conventionally, when measuring the sperm concentration with an optical microscope based on the WHO guidelines, it is necessary to measure with a constant semen thickness depending on the instrument on which the semen sample is placed (coated).
For example, depending on the slide glass and cover glass, or in the McLar sperm analysis count chamber, the thickness of the semen is accurately made constant by two parts of the bottom of the container and the cover glass on the top. However, if a simple microscope used in the present invention consisting of a single high-magnification objective lens is used, a special member for making the thickness constant is unnecessary because the depth of field is extremely shallow, and semen is applied to a transparent sheet. Since it is only necessary to record with a digital camera such as a portable terminal, it is possible to obtain the sperm concentration very easily and inexpensively, and the transparent sheet with pseudo ovum projection of the present invention and the number of sperm to be counted using the same It is possible to obtain information about the possibility of infertility to some extent by individuals without shame, and to open the way to starting early infertility treatment, and to solve the problem of infertility worldwide It may be insignificant, but it seems to be able to contribute.

1 is a schematic plan view of one embodiment of the present invention. 1 is a schematic side view of an embodiment of the present invention. 1 is a schematic side view of an embodiment of the present invention. 1 is a schematic cross-sectional view of an embodiment of the present invention. Illustration of a simple microscope used in the present invention Schematic sectional view of a simple microscope used in the present invention Illustration of the template of the present invention Illustration of the template of the present invention

The simple microscope used in the present invention is the one shown in FIG. 5, and the objective lens 6 is superposed on the lens holding plate 5 and the sheet holding plate 7 on which the semen coating transparent sheet 8 is attached to the opening 9. FIG. 5 shows a simple microscope composed of a single objective lens that allows the semen coating transparent sheet 8 to be brought into surface contact as shown in FIG. 6 to see the state of sperm. FIG. 5 shows that the semen coating transparent sheet 8 is fixed to a digital camera such as a portable terminal. 6 is a part of the lens holding plate 5, and the transparent sheet holding plate 7 can be freely moved, attached and detached.
The mode for carrying out the invention is as follows. As shown by reference numeral 1 in FIG. 1, a transparent sheet having a thickness of about 0.05 mm and a size of about 15 × 20 mm or a diameter of about 20 mm is made of a synthetic material that is softer than the lens. A glass or polystyrene spherical bead 2 having a diameter of about 0.05 to 0.1 mm is fixed as a resin pseudo-egg by fusing, adhering or sticking as a sperm obstacle protrusion as shown in the side view of FIG. A sperm obstruction that has a diameter of about 0.05 to 0.1 mm or a square or a thickness of about 0.01 mm on the transparent sheet as shown in the side view of FIG. This is a product made by molding a substrate with a thickness of 0.05mm using a nano-imprint technology as a projection, using a nanoimprint technology. It is over door.
Further, since the transparent sheet with pseudo-egg protrusions is thin as it is, it is difficult to handle, so it is stuck on the transparent sheet holding plate 4 (same as reference numeral 7 in FIG. 5) as shown in the schematic sectional view of FIG. Use it.
Hereinafter, details of the present invention will be described with reference to the drawings.

FIG. 1 is a schematic plan view of an embodiment of a transparent sheet with pseudo ovum projections of the present invention. A glass spherical bead 2 having a diameter of 0.07 mm as a pseudo ovum has a thickness of 0.05 mm as shown in the schematic side view of FIG. It is a thing fixed to the transparent adhesive tape made from polyester.

FIG. 3 shows a transparent sheet with pseudo-oval projections of the present invention in which a 0.1 × 0.1 mm square polyethylene film having a thickness of 0.05 mm is fixed to a transparent adhesive tape made of polyester having a thickness of 0.05 mm. This is one example. In addition, this embodiment is a transparent PET having a thickness of 0.01 mm and a thickness of 0.05 mm, which is a base of a round or square embossed protrusion having a diameter or a side of about 0.05 to 0.1 mm. The sheet can be molded with a photo-curable resin using nanoimprint technology. If the side surface of the emboss is perpendicular to the surface of the transparent sheet 1, the magnification of the simple microscope is different, and the cross-sectional shape of the image side of the objective lens of the simple microscope is constant even if the focal position changes. It can be used as a gauge having a large area.

FIG. 4 is a product in which the transparent sheet 1 with pseudo ovum projection of the present invention shown in FIG. 2 is attached to the opening 9 of the transparent sheet holding plate 4, thereby handling the transparent sheet with pseudo ovum projection, The damage to the protrusions is prevented and the utility is further increased. In use, the semen to be observed is applied from the opening 9, and the objective lens of the simple microscope is brought into surface contact with the surface opposite to the semen application surface of the transparent sheet 1 and observed as in the cross section of FIG.

FIG. 7 and FIG. 8 are diagrams of an embodiment of a template with a hole used for counting the number of sperm used as a basis for calculating the sperm concentration using the camera function of a simple microscope and a portable terminal. , 10 in the figure is a transparent PVC sheet having a rectangular shape of 100 × 70 mm and a thickness of 0.2 mm, and a hole of circular 11 or square 112 is formed in the sheet, and the hole has the same shape and 9 times the area corresponding to the hole. The template provided with 12 or 122 is shown. That is, one transparent sheet with a small round hole and a large round hole, or one transparent sheet with a small square hole and a large square hole is shown in FIG. FIG. 8 shows a transparent sheet opened.
The small hole is a round image of the monitor screen when the projection of the transparent sheet with pseudo ovum projection of the present invention is photographed with a digital camera such as a portable terminal device through the objective lens of a simple microscope, for example, FIG. In the case of 110 out of 8, it may be scaled up or down so as to be the same as the round hole 11 of the area figure larger than the image, or it may be matched to the square hole 112 so as to be an inscribed circle. If the cross-sectional shape of the protrusion is square, it is aligned with the hole 112. The area of the small hole template is equal to or larger than the image of the pseudo-oval projection on most portable terminals commercially available.

  If the present invention is recommended in a hospital, it is possible to send an image from a home to a hospital with a portable terminal and receive a medical examination, etc., and a man's shame can be eased.

Reference numeral 1 denotes a transparent sheet 2, and spherical beads 3 denote circular or square plate-like bodies or embossed protrusions.
4 is a transparent sheet holding plate 5 with a pseudo ovum projection 5 lens holding plate 6 objective lens 7 transparent sheet holding plate 8 transparent sheet 9 opening 10 transparent sheet 11 small round hole 12 large round hole 110 The image 112 of the handheld terminal with a circular pseudo-oval projection is a small square hole 122 is a large square hole

Claims (12)

Providing a projection that is an obstacle for sperm on the side of the transparent sheet that does not come into contact with the lens for exclusive use with a simple microscope consisting of a single objective lens for observation by bringing the objective lens into contact with one side of the transparent sheet A transparent sheet with pseudo-oval projections. The transparent sheet with pseudo-egg projections according to claim 1, wherein the transparent sheet forms a part of a transparent bag. 2. The transparent sheet with pseudo-egg projections according to claim 1, wherein the projection has a substantially circular cross-sectional shape. The transparent sheet with pseudo ovum protrusions according to claim 1, wherein the protrusion has a substantially square cross-sectional shape. The transparent sheet with pseudo ovum protrusions according to claim 1, wherein the protrusions are spherical beads. 2. The transparent sheet with pseudo-egg projections according to claim 1, wherein the transparent sheet is a transparent adhesive tape, and spherical beads are fixed to the adhesive side. The transparent sheet with pseudo ovum protrusions according to claim 1, wherein the transparent sheet is a transparent adhesive tape, and a square flat plate of about 0.05 to 0.1 mm is fixed to the adhesive side. The semen is applied to the protrusion side of the transparent sheet with pseudo ovum projection according to claim 1, and the objective lens of a simple microscope consisting of one objective lens is brought into contact with the surface opposite to the surface of the sperm so that the behavior of the sperm The observation method using the transparent sheet | seat with a pseudo ovulation to observe. The sperm in the semen applied to the transparent sheet with pseudo-ovulations was used as a transparent sheet with pseudo-ovulations, with the total number of sperm trying to enter the protrusions per unit time as an index value of the male fertility Observation method. A small sheet and a large hole corresponding to the image of the protrusion are opened in a transparent sheet that is large enough to be placed on the monitor screen of a digital camera in a portable terminal with a predetermined ratio that does not protrude from the frame of the monitor screen. The observation method using the transparent sheet | seat with a pseudo ovum process characterized by using the template for the count of the spermatozoa performed. The template according to claim 10, wherein the size of the monitor image of a digital camera such as a portable terminal through the simple microscope of the protrusion having a cross section of the transparent sheet with pseudo ovum protrusion according to claim 3. Sperm for counting the number of spermatozoa in the larger hole while maintaining the same condition, with the overall magnification of the digital camera, such as a simple microscope and a portable terminal, fixed to the same size as the smaller hole The observation method using the transparent sheet | seat with a pseudo ovulation characterized by using the template for several counts. The pseudo-oval projections on the transparent sheet with pseudo-oval projections are regarded as a magnification determining gauge, and the size of the gauge on the monitor image of a digital camera, such as a portable terminal, is adjusted to a figure with a predetermined area through a simple microscope. Then, using a transparent sheet with pseudo-oval projections, characterized in that the total magnification of a digital camera such as a simple microscope and a portable terminal is determined to be constant, and the number of sperm in a figure larger than the figure of the predetermined area is counted. Was the observation method.

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