JP2016514957A5 - - Google Patents
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- JP2016514957A5 JP2016514957A5 JP2015561647A JP2015561647A JP2016514957A5 JP 2016514957 A5 JP2016514957 A5 JP 2016514957A5 JP 2015561647 A JP2015561647 A JP 2015561647A JP 2015561647 A JP2015561647 A JP 2015561647A JP 2016514957 A5 JP2016514957 A5 JP 2016514957A5
- Authority
- JP
- Japan
- Prior art keywords
- host cell
- cell according
- recombinant polypeptide
- acid sequence
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000004027 cells Anatomy 0.000 claims 22
- 229920001184 polypeptide Polymers 0.000 claims 11
- 150000007523 nucleic acids Chemical group 0.000 claims 5
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 4
- 102100001249 ALB Human genes 0.000 claims 2
- 101710027066 ALB Proteins 0.000 claims 2
- 101700063252 ERO1 Proteins 0.000 claims 2
- 102000033147 ERVK-25 Human genes 0.000 claims 2
- 101700023713 PADI1 Proteins 0.000 claims 2
- 108091005771 Peptidases Proteins 0.000 claims 2
- 241000235648 Pichia Species 0.000 claims 2
- 239000004365 Protease Substances 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 229940050528 albumin Drugs 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- DTHNMHAUYICORS-KTKZVXAJSA-N 107444-51-9 Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC=1N=CNC=1)[C@@H](C)O)[C@@H](C)O)C(C)C)C1=CC=CC=C1 DTHNMHAUYICORS-KTKZVXAJSA-N 0.000 claims 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims 1
- 210000002472 Endoplasmic Reticulum Anatomy 0.000 claims 1
- 101710042131 GCG Proteins 0.000 claims 1
- 101700071595 GRZ1 Proteins 0.000 claims 1
- 241000235649 Kluyveromyces Species 0.000 claims 1
- 101700076891 PAPA Proteins 0.000 claims 1
- 102000006010 Protein Disulfide-Isomerases Human genes 0.000 claims 1
- 108020003519 Protein Disulfide-Isomerases Proteins 0.000 claims 1
- 241000235070 Saccharomyces Species 0.000 claims 1
- 241000235346 Schizosaccharomyces Species 0.000 claims 1
- 241000311088 Schwanniomyces Species 0.000 claims 1
- 101710021109 UBE2D2 Proteins 0.000 claims 1
- 101700052173 YPS1 Proteins 0.000 claims 1
- 241000235013 Yarrowia Species 0.000 claims 1
- 102100005236 ZGLP1 Human genes 0.000 claims 1
- 101700078733 ZGLP1 Proteins 0.000 claims 1
- 102000037240 fusion proteins Human genes 0.000 claims 1
- 108020001507 fusion proteins Proteins 0.000 claims 1
- 230000005017 genetic modification Effects 0.000 claims 1
- 235000013617 genetically modified food Nutrition 0.000 claims 1
- 239000001963 growth media Substances 0.000 claims 1
- 230000002147 killing Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 101710013679 ogm1 Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000001225 therapeutic Effects 0.000 claims 1
- 101700085620 ubc4 Proteins 0.000 claims 1
Claims (14)
- キラーエクスプレッションプロテアーゼ（Ｋｅｘ２ｐ）またはその断片および／もしくは変異体をコードする少なくとも１つの異種核酸配列と、小胞体オキシドレダクチン（Ｅｒｏ１）またはその断片および／もしくは変異体をコードする少なくとも１つの異種核酸配列と、タンパク質ジスルフィドイソメラーゼ（Ｐｄｉ１）またはその断片および／もしくは変異体をコードする少なくとも１つの異種核酸配列とを含んでなる宿主細胞。
- 前記宿主細胞が培養系で増殖される際に、前記宿主細胞がＫｅｘ２ｐ、Ｐｄｉ１およびＥｒｏ１から選択されるタンパク質またはその断片および／もしくは変異体をコードする前記少なくとも１つの異種核酸配列の少なくとも１つの遺伝子産物を発現または過剰発現する、請求項１に記載の宿主細胞。
- 前記宿主細胞が酵母細胞である、請求項１または２に記載の宿主細胞。
- 前記酵母細胞の属がサッカロミセス、クルイベロミセス、カンジダ、ピキア、シゾサッカロミセス、ハンセヌラ、クロエケラ、シュワンニオミセス、およびヤロウイアからなる群から選択される、請求項３に記載の宿主細胞。
- 前記宿主細胞がＳ．セレビシエである、請求項１〜４のいずれか一項に記載の宿主細胞。
- 前記宿主細胞が下記遺伝子改変：ｐｅｐ４プロテアーゼノックアウト、野生型宿主細胞に比べて低いｕｂｃ４および／またはｕｂｃ５活性、ｙｐｓ１ノックアウト、ｈｓｐ１５０ノックアウト、ならびにｐｍｔ１ノックアウトのうち少なくとも１つをさらに含んでなる、請求項１〜５のいずれか一項に記載の宿主細胞。
- 組換えポリペプチドをコードする少なくとも１つの核酸を含んでなる、請求項１〜６のいずれか一項に記載の宿主細胞。
- 前記組換えポリペプチドが少なくとも１つのジスルフィド結合を有する、請求項７に記載の宿主細胞。
- 前記組換えポリペプチドがアルブミン融合タンパク質である、請求項７または８に記載の宿主細胞。
- 前記組換えポリペプチドが、アルブミンに遺伝子学的に融合された、ＧＬＰ−１活性を有する少なくとも１つの治療用ポリペプチドを含んでなる、請求項７〜９のいずれか一項に記載の宿主細胞。
- 前記組換えポリペプチドが、配列番号１に示されるアミノ酸配列と少なくとも９５％の配列同一性を有するポリペプチドを有する、請求項７〜１０のいずれか一項に記載の宿主細胞。
- 前記組換えポリペプチドが、配列番号１に示されるアミノ酸配列を含んでなる、請求項７〜１１のいずれか一項に記載の宿主細胞。
- 前記組換えポリペプチドがリーダー配列を含んでなる、請求項７〜１２のいずれか一項に記載の宿主細胞。
- 請求項７〜１３のいずれか一項に記載の宿主細胞を培養すること、および、培養培地から前記組換えポリペプチドを回収することを含んでなる、組換えポリペプチドを生産する方法。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361773329P | 2013-03-06 | 2013-03-06 | |
| US61/773,329 | 2013-03-06 | ||
| PCT/US2014/021137 WO2014138371A1 (en) | 2013-03-06 | 2014-03-06 | Host cells and methods of use |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019044077A Division JP2019122395A (ja) | 2013-03-06 | 2019-03-11 | 宿主細胞および使用方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016514957A JP2016514957A (ja) | 2016-05-26 |
| JP2016514957A5 true JP2016514957A5 (ja) | 2017-04-13 |
| JP6742729B2 JP6742729B2 (ja) | 2020-08-19 |
Family
ID=51491943
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015561647A Active JP6742729B2 (ja) | 2013-03-06 | 2014-03-06 | 宿主細胞および使用方法 |
| JP2019044077A Pending JP2019122395A (ja) | 2013-03-06 | 2019-03-11 | 宿主細胞および使用方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019044077A Pending JP2019122395A (ja) | 2013-03-06 | 2019-03-11 | 宿主細胞および使用方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9926570B2 (ja) |
| EP (1) | EP2964666B1 (ja) |
| JP (2) | JP6742729B2 (ja) |
| KR (1) | KR20150126839A (ja) |
| AU (1) | AU2014225678B2 (ja) |
| BR (1) | BR112015021960A2 (ja) |
| CA (1) | CA2903739A1 (ja) |
| ES (1) | ES2843679T3 (ja) |
| RU (1) | RU2663587C2 (ja) |
| SG (1) | SG11201504771RA (ja) |
| WO (1) | WO2014138371A1 (ja) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3016970B1 (en) | 2013-07-04 | 2019-05-22 | Glykos Finland Oy | O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof |
| WO2016012468A1 (en) | 2014-07-21 | 2016-01-28 | Novartis Ag | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| CN108431204A (zh) | 2015-12-22 | 2018-08-21 | 阿尔布梅迪克斯医疗有限公司 | 改良的表达蛋白质的菌株 |
| CN105950491B (zh) * | 2016-05-23 | 2019-08-06 | 江南大学 | 一种高效表达碱性果胶酶的菌株及其构建与应用 |
| JP7282693B2 (ja) | 2017-06-20 | 2023-05-29 | アルブミディクス リミティド | 改良されたタンパク質発現株 |
| KR101984957B1 (ko) * | 2017-08-09 | 2019-06-04 | 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 | 효모의 단백질 접힘 기작(protein-folding machinery) 개량을 통한 진세노사이드 생산 증대 |
| US11135276B2 (en) | 2019-05-17 | 2021-10-05 | Imam Abdulrahman Bin Faisal University | Method of making PDIA2 and compositions containing PDIA2 |
| KR20220031543A (ko) | 2019-06-28 | 2022-03-11 | 미츠비시 가스 가가쿠 가부시키가이샤 | 수지 조성물, 수지 시트, 적층체, 수지 조성물층 부착 반도체 웨이퍼, 수지 조성물층 부착 반도체 탑재용 기판, 및 반도체 장치 |
| EP4402257A1 (en) * | 2021-09-17 | 2024-07-24 | The University of North Carolina at Chapel Hill | Modified protein disulfide isomerase and uses thereof |
| GB202301988D0 (en) * | 2023-02-13 | 2023-03-29 | Phenotypeca Ltd | Breeding process |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5077204A (en) | 1984-06-21 | 1991-12-31 | Chiron Corporation | Yeast endopeptidase for basic amino-acid site cleavage, preparation and use |
| JP2643968B2 (ja) | 1988-02-03 | 1997-08-25 | サントリー株式会社 | Ｋｅｘ２エンドプロテアーゼ及びその製造方法 |
| FR2645174B1 (fr) | 1989-03-31 | 1994-01-07 | Transgene Sa | Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant |
| US5545618A (en) | 1990-01-24 | 1996-08-13 | Buckley; Douglas I. | GLP-1 analogs useful for diabetes treatment |
| GB9015825D0 (en) | 1990-07-18 | 1990-09-05 | Ciba Geigy Ag | In vitro processing of fusion proteins |
| ATE157703T1 (de) | 1991-12-16 | 1997-09-15 | Ciba Geigy Ag | Endoplasmatisches retikulum-ständige rekombinante dibasische endoprotease und deren verwendungen |
| US6291205B1 (en) | 1992-06-12 | 2001-09-18 | Merck & Co., Inc. | Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae |
| GB9404270D0 (en) | 1994-03-05 | 1994-04-20 | Delta Biotechnology Ltd | Yeast strains and modified albumins |
| GB9411356D0 (en) * | 1994-06-07 | 1994-07-27 | Delta Biotechnology Ltd | Yeast strains |
| CA2198968C (en) | 1996-03-04 | 2010-02-09 | Toyofumi Masuda | Process for production of secretory kex2 derivatives |
| IL127583A0 (en) * | 1996-07-05 | 1999-10-28 | Novo Nordisk As | Method for the production of polypeptides |
| US7504493B2 (en) | 1997-01-23 | 2009-03-17 | The John Hopkins University | Characterization of the yeast transcriptome |
| GB9713412D0 (en) | 1997-06-26 | 1997-08-27 | Delta Biotechnology Ltd | Improved protein expression strains |
| WO1999007727A1 (en) * | 1997-08-12 | 1999-02-18 | Massachusetts Institute Of Technology | Eukaryotic disulfide bond-forming proteins and related molecules and methods |
| IL127127A0 (en) | 1998-11-18 | 1999-09-22 | Peptor Ltd | Small functional units of antibody heavy chain variable regions |
| GB9902000D0 (en) * | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
| EP2990417A1 (en) * | 2001-12-21 | 2016-03-02 | Human Genome Sciences, Inc. | Albumin insulin fusion protein |
| PL1729795T3 (pl) | 2004-02-09 | 2016-08-31 | Human Genome Sciences Inc | Białka fuzyjne albuminy |
| US20060046253A1 (en) * | 2004-09-02 | 2006-03-02 | Suntory Limited | Method for analyzing genes of industrial yeasts |
| ATE411384T1 (de) | 2005-05-04 | 2008-10-15 | Signalomics Gmbh | Kompetitives n-hybrid system |
| JP2009514900A (ja) * | 2005-11-04 | 2009-04-09 | スミスクライン・ビーチャム・コーポレイション | 血糖降下剤の投与方法 |
| JP5354559B2 (ja) | 2005-11-24 | 2013-11-27 | 独立行政法人産業技術総合研究所 | 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系 |
| DK2046951T5 (da) | 2006-07-05 | 2012-01-23 | Catalyst Biosciences Inc | Proteasesreeningsmetoder og proteaser identificeret dermed |
| US8034607B2 (en) * | 2007-09-12 | 2011-10-11 | Wisconsin Alumni Research Foundation | Methods of enhanced heterologous protein secretion |
| EP2210940B2 (en) * | 2007-10-31 | 2018-07-18 | Daiichi Sankyo Company, Limited | Method for high-level secretion and production of protein |
| JP2011507507A (ja) | 2007-12-21 | 2011-03-10 | フジフィルム・マニュファクチュアリング・ヨーロッパ・ベスローテン・フエンノートシャップ | マルチマー性前駆体のｉｎｖｉｖｏタンパク質分解的プロセシングによる関心対象のタンパク質の分泌収量の改善 |
| US20110129872A1 (en) | 2009-12-01 | 2011-06-02 | Mogam Biotechnology Research Institute | Method for a production of a recombinant protein using yeast co-expression system |
| RU2427645C1 (ru) * | 2010-06-10 | 2011-08-27 | Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") | СПОСОБ МИКРОБИОЛОГИЧЕСКОГО СИНТЕЗА ЗРЕЛОГО ИНТЕРФЕРОНА АЛЬФА-2 ЧЕЛОВЕКА, ШТАММ Saccharomyces cerevisiae - ПРОДУЦЕНТ ЗРЕЛОГО ИНТЕРФЕРОНА АЛЬФА-2 ЧЕЛОВЕКА (ВАРИАНТЫ) |
| JP6487328B2 (ja) * | 2012-11-08 | 2019-03-20 | アルブミディクス リミティド | アルブミン変異体 |
-
2014
- 2014-03-06 AU AU2014225678A patent/AU2014225678B2/en not_active Ceased
- 2014-03-06 RU RU2015135980A patent/RU2663587C2/ru not_active IP Right Cessation
- 2014-03-06 ES ES14760330T patent/ES2843679T3/es active Active
- 2014-03-06 CA CA2903739A patent/CA2903739A1/en not_active Abandoned
- 2014-03-06 JP JP2015561647A patent/JP6742729B2/ja active Active
- 2014-03-06 BR BR112015021960A patent/BR112015021960A2/pt not_active Application Discontinuation
- 2014-03-06 EP EP14760330.2A patent/EP2964666B1/en active Active
- 2014-03-06 WO PCT/US2014/021137 patent/WO2014138371A1/en active Application Filing
- 2014-03-06 SG SG11201504771RA patent/SG11201504771RA/en unknown
- 2014-03-06 US US14/772,436 patent/US9926570B2/en active Active
- 2014-03-31 KR KR1020157023780A patent/KR20150126839A/ko not_active Application Discontinuation
-
2019
- 2019-03-11 JP JP2019044077A patent/JP2019122395A/ja active Pending
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