JP2014528726A - デヒドロゲナーゼ変異体およびこれをコードするポリヌクレオチド - Google Patents

デヒドロゲナーゼ変異体およびこれをコードするポリヌクレオチド Download PDF

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JP2014528726A
JP2014528726A JP2014533652A JP2014533652A JP2014528726A JP 2014528726 A JP2014528726 A JP 2014528726A JP 2014533652 A JP2014533652 A JP 2014533652A JP 2014533652 A JP2014533652 A JP 2014533652A JP 2014528726 A JP2014528726 A JP 2014528726A
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seq
variant
ala
host cell
polypeptide
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Japanese (ja)
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タソネ モニカ
タソネ モニカ
デ マリア レオナルド
デ マリア レオナルド
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ノボザイムス，インコーポレイティド
ノボザイムス アクティーゼルスカブ
ノボザイムス アクティーゼルスカブ
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Publication of JP2014528726A publication Critical patent/JP2014528726A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01059—3-Hydroxypropionate dehydrogenase (1.1.1.59)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01276—Serine 3-dehydrogenase (1.1.1.276)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01298—3-Hydroxypropionate dehydrogenase (NADP+) (1.1.1.298)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2014533652A 2011-09-30 2012-09-25 デヒドロゲナーゼ変異体およびこれをコードするポリヌクレオチド Pending JP2014528726A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161541363P 2011-09-30 2011-09-30
US61/541,363 2011-09-30
PCT/US2012/057134 WO2013049073A1 (en) 2011-09-30 2012-09-25 Dehydrogenase variants and polynucleotides encoding same

Related Child Applications (1)

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JP2017166889A Division JP2018033457A (ja) 2011-09-30 2017-08-31 デヒドロゲナーゼ変異体およびこれをコードするポリヌクレオチド

Publications (1)

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JP2014528726A true JP2014528726A (ja) 2014-10-30

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JP2014533652A Pending JP2014528726A (ja) 2011-09-30 2012-09-25 デヒドロゲナーゼ変異体およびこれをコードするポリヌクレオチド
JP2017166889A Pending JP2018033457A (ja) 2011-09-30 2017-08-31 デヒドロゲナーゼ変異体およびこれをコードするポリヌクレオチド
JP2019046076A Pending JP2019110916A (ja) 2011-09-30 2019-03-13 デヒドロゲナーゼ変異体およびこれをコードするポリヌクレオチド

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JP2017166889A Pending JP2018033457A (ja) 2011-09-30 2017-08-31 デヒドロゲナーゼ変異体およびこれをコードするポリヌクレオチド
JP2019046076A Pending JP2019110916A (ja) 2011-09-30 2019-03-13 デヒドロゲナーゼ変異体およびこれをコードするポリヌクレオチド

Country Status (10)

Country Link
US (3) US8728788B1 (forum.php)
EP (1) EP2751261B1 (forum.php)
JP (3) JP2014528726A (forum.php)
CN (1) CN103857791B (forum.php)
AU (1) AU2012316246B2 (forum.php)
BR (1) BR112014007647A2 (forum.php)
CA (1) CA2849303C (forum.php)
DK (1) DK2751261T3 (forum.php)
IN (1) IN2014CN03187A (forum.php)
WO (1) WO2013049073A1 (forum.php)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2849303C (en) * 2011-09-30 2019-09-17 Novozymes, Inc. Dehydrogenase variants and polynucleotides encoding same
WO2015017721A1 (en) 2013-07-31 2015-02-05 Novozymes A/S 3-hydroxypropionic acid production by recombinant yeasts expressing an insect aspartate 1-decarboxylase
US20180273915A1 (en) * 2014-12-19 2018-09-27 Novozymes A/S Recombinant Host Cells For The Production Of 3-Hydroxypropionic Acid
WO2016154046A2 (en) 2015-03-20 2016-09-29 Verdezyne, Inc. Biological methods for preparing 3-hydroxypropionic acid
US20180265902A1 (en) 2015-08-24 2018-09-20 Novozymes A/S Beta-Alanine Aminotransferases For The Production of 3-Hydroxypropionic Acid
WO2023168244A1 (en) * 2022-03-03 2023-09-07 Cargill, Incorporated Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate
WO2023168233A1 (en) * 2022-03-03 2023-09-07 Cargill, Incorporated Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate
KR102782737B1 (ko) * 2022-11-29 2025-03-19 씨제이제일제당 주식회사 신규한 프로피온알데하이드 디하이드로게나제 활성을 갖는 변이체 폴리펩티드 및 이의 용도

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008027742A1 (en) * 2006-08-30 2008-03-06 Cargill, Incorporated Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production
JP2012085635A (ja) * 2010-03-23 2012-05-10 Nippon Shokubai Co Ltd ３−ヒドロキシプロピオン酸の製造方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL97645A (en) 1990-03-23 1997-03-18 Gist Brocades Nv Production of enzymes in seeds and their use
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
CA2191718A1 (en) 1994-06-03 1995-12-14 Randy M. Berka Phosphonyldipeptides useful in the treatment of cardiovascular diseases
JP3167729B2 (ja) 1994-06-30 2001-05-21 ノボ ノルディスク バイオテック，インコーポレイティド 無毒、非毒素原性、非病原性の発現系、並びにその中で利用するためのプロモーター及びターミネーター
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
ATE332968T1 (de) 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
CN1940067A (zh) 1999-03-22 2007-04-04 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
WO2001016346A1 (en) 1999-08-30 2001-03-08 Wisconsin Alumni Research Foundation Production of 3-hydroxypropionic acid in recombinant organisms
CA2429039A1 (en) 2000-11-20 2002-05-30 Cargill, Incorporated 3-hydroxypropionic acid and other organic compounds
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
ATE375388T1 (de) 2001-07-27 2007-10-15 Us Gov Health & Human Serv Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden
CA2473716A1 (en) 2002-01-18 2003-07-31 Cargill, Incorporated Alanine 2,3- aminomutase
WO2003082795A2 (en) 2002-03-25 2003-10-09 Cargill, Incorporated METHODS OF MANUFACTURING DERIVATIVES OF β-HYDROXYCARBOXYLIC ACIDS
ATE543873T1 (de) * 2003-12-04 2012-02-15 Novozymes As Herstellung von 3-hydroxypropionsäure mittels beta-alanine-pyruvat-aminotrransferase
AU2004322803A1 (en) 2004-07-30 2006-03-02 Cargill, Incorporated Alanine 2, 3 aminomutases
EP1805304A2 (en) 2004-10-25 2007-07-11 Codexis, Inc. Improved alanine 2,3-aminomutases and related polynucleotides
DE102005048818A1 (de) 2005-10-10 2007-04-12 Degussa Ag Mikrobiologische Herstellung von 3-Hydroxypropionsäure
CA2675026A1 (en) 2007-01-12 2008-07-24 The Regents Of The University Of Colorado, A Body Corporate Compositions and methods for enhancing tolerance for the production of organic chemicals produced by microorganisms
US8673601B2 (en) 2007-01-22 2014-03-18 Genomatica, Inc. Methods and organisms for growth-coupled production of 3-hydroxypropionic acid
WO2009089457A1 (en) 2008-01-11 2009-07-16 Novozymes A/S Methods for producing 3-hydroxypropionic acid and compounds thereof
US20100021978A1 (en) 2008-07-23 2010-01-28 Genomatica, Inc. Methods and organisms for production of 3-hydroxypropionic acid
EP2326709A4 (en) 2008-09-15 2013-01-23 Opx Biotechnologies Inc METHODS, SYSTEMS AND COMPOSITIONS RELATED TO REDUCED MICROBIAL PRODUCTION OF 3-HYDROXYPROPIONIC ACID (3-HP) IN ALDEHYDMETABOLITE
US20100151345A1 (en) 2008-12-17 2010-06-17 Saint-Gobain Ceramics & Plastics, Inc. Electrode Gas Channel Supports and Methods for Forming Internal Channels
CA2775390C (en) * 2009-09-27 2021-06-29 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
DK2534240T3 (da) * 2010-02-12 2019-08-05 Gevo Inc Gærmikroorganismer med reduceret biprodukt-akkumulering til forbedret fremstilling af brændstoffer, kemikalier og aminosyrer
WO2012074818A2 (en) 2010-11-22 2012-06-07 Novozymes, Inc. Compositions and methods for 3-hydroxypropionic acid production
WO2013043758A2 (en) 2011-09-19 2013-03-28 Opx Biotechnologies, Inc. Compositions and methods regarding direct nadh utilization to produce 3-hydroxypropionic acid, derived chemicals and further derived products
CA2849303C (en) * 2011-09-30 2019-09-17 Novozymes, Inc. Dehydrogenase variants and polynucleotides encoding same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008027742A1 (en) * 2006-08-30 2008-03-06 Cargill, Incorporated Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production
JP2012085635A (ja) * 2010-03-23 2012-05-10 Nippon Shokubai Co Ltd ３−ヒドロキシプロピオン酸の製造方法

Also Published As

Publication number Publication date
AU2012316246B2 (en) 2017-10-26
EP2751261A1 (en) 2014-07-09
US9163220B2 (en) 2015-10-20
CN103857791B (zh) 2016-11-23
US9404091B2 (en) 2016-08-02
BR112014007647A2 (pt) 2017-04-18
CA2849303C (en) 2019-09-17
WO2013049073A1 (en) 2013-04-04
US20140212938A1 (en) 2014-07-31
IN2014CN03187A (forum.php) 2015-07-03
US8728788B1 (en) 2014-05-20
JP2018033457A (ja) 2018-03-08
CA2849303A1 (en) 2013-04-04
US20160017296A1 (en) 2016-01-21
JP2019110916A (ja) 2019-07-11
AU2012316246A1 (en) 2014-03-20
DK2751261T3 (en) 2018-12-10
CN103857791A (zh) 2014-06-11
EP2751261B1 (en) 2018-08-29

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