JP2013511280A5 - - Google Patents
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- JP2013511280A5 JP2013511280A5 JP2012540120A JP2012540120A JP2013511280A5 JP 2013511280 A5 JP2013511280 A5 JP 2013511280A5 JP 2012540120 A JP2012540120 A JP 2012540120A JP 2012540120 A JP2012540120 A JP 2012540120A JP 2013511280 A5 JP2013511280 A5 JP 2013511280A5
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- 238000006467 substitution reaction Methods 0.000 claims 34
- 101700012728 BGL1 Proteins 0.000 claims 33
- 101710004392 BGLU18 Proteins 0.000 claims 33
- 101710027083 KLLA0C05324g Proteins 0.000 claims 31
- 101710025233 LLGL1 Proteins 0.000 claims 31
- 230000000694 effects Effects 0.000 claims 16
- 230000000875 corresponding Effects 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 102000006995 beta-Glucosidase Human genes 0.000 claims 3
- 108010047754 beta-Glucosidase Proteins 0.000 claims 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims 3
- 238000006460 hydrolysis reaction Methods 0.000 claims 3
- 101710004381 BGLU1 Proteins 0.000 claims 2
- 239000002028 Biomass Substances 0.000 claims 2
- 101710003491 GLU1 Proteins 0.000 claims 2
- 101700039703 MYRO1 Proteins 0.000 claims 2
- 241000209149 Zea Species 0.000 claims 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 235000005822 corn Nutrition 0.000 claims 2
- 235000005824 corn Nutrition 0.000 claims 2
- 240000007582 Corylus avellana Species 0.000 claims 1
- 235000007466 Corylus avellana Nutrition 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 230000003301 hydrolyzing Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000001105 regulatory Effects 0.000 claims 1
- 239000010907 stover Substances 0.000 claims 1
Claims (23)
- 配列番号３に少なくとも８０％のアミノ酸配列同一性を有するβ−グルコシダーゼ１（ＢＧＬ１）変異体において、
（ａ）前処理済みトウモロコシ茎葉（ＰＣＳ）加水分解活性、
（ｂ）セロビアーゼ活性、
（ｃ）タンパク質発現、
（ｄ）アンモニアで前処理したトウモロコシ穂軸（ＣＣ）の存在下でのセロビアーゼ活
性又はＣＣ加水分解活性のいずれかにより測定されるβ−グルコシダーゼ活性、
（ｅ）熱安定性、
（ｆ）リン酸膨潤セルロース（ＰＡＳＣ）加水分解活性、及び
（ｇ）グルコース存在下での加水分解活性、
からなる群から選択される少なくとも２つの活性が配列番号３のアミノ酸配列を有する野生型β−グルコシダーゼ１（ＢＧＬ１）よりも改良されており、
上記ＢＧＬ１変異体が配列番号３のＩ５６７、Ｑ２２６、Ｅ１７０、Ｎ２６３、Ｓ６８３、及びＮ２３８から選択される位置に対応する位置における置換を含むＢＧＬ１変異体。 - 請求項１に記載のＢＧＬ１変異体において、
（１）置換位置がＱ２２６である場合、前記置換はＱ２２６Ｙ又はＣ２２６Ｆであり、
（２）置換位置がＥ１７０である場合、前記置換はＥ１７０Ｆであり、
（３）置換位置がＮ２６３である場合、前記置換はＮ２６３Ｃ又はＮ２６３Ｔであり、
（４）置換位置がＳ６８３である場合、前記置換はＳ６８３Ｗであり、
（５）置換位置がＮ２３８である場合、前記置換はＮ２３８Ｆである、ＢＧＬ１変異体。 - 請求項２に記載のＢＧＬ１変異体において、該変異体が（１）の置換を有する場合、更に配列番号３のＥ１７０、Ｔ２４２、Ｓ３１２、Ｎ３６９、Ｇ３７２、Ｖ６０３、Ｐ６６１、及び／又はＴ６６６に対応する位置における１つ以上の置換を更に含むＢＧＬ１変異体。
- 請求項３に記載のＢＧＬ１変異体において、前記置換がＥ１７０Ｆ、Ｔ２４２Ｓ，Ｓ３１２Ｙ、Ｎ３６９Ｙ、Ｇ３７２Ａ、Ｖ６０３Ｇ、Ｐ６６１Ｆ、及び／又はＴ６６６ＣであるＢＧＬ１変異体。
- 請求項２に記載のＢＧＬ１変異体において、該変異体が（２）の置換を有する場合、更に配列番号３のＱ２２６、Ｔ２４２，Ｓ３１２、Ｎ３６９、Ｇ３７２、Ｖ６０３、Ｐ６６１、及び／又はＴ６６６に対応する位置における１つ以上の置換を更に含むＢＧＬ１変異体。
- 請求項５に記載のＢＧＬ１変異体において、前記置換がＱ２２６Ｙ、Ｔ２４２Ｓ，Ｓ３１２Ｙ、Ｎ３６９Ｙ、Ｇ３７２Ａ、Ｖ６０３Ｇ、Ｐ６６１Ｆ、及び／又はＴ６６６ＣであるＢＧＬ１変異体。
- 請求項２に記載のＢＧＬ１変異体において、該変異体が（３）の置換を有する場合、更に配列番号３のＫ３４５、Ｎ３６９、Ｇ３７２、Ｋ４２８、Ｐ６６１、及び／又はＳ６８３に対応する位置における１つ以上の置換を更に含むＢＧＬ１変異体。
- 請求項７に記載のＢＧＬ１変異体において、前記置換がＫ３４５Ｅ、Ｎ３６９Ｅ、Ｎ３６９Ｔ、Ｇ３７２Ａ、Ｋ４２８Ｎ、Ｐ６６１Ｅ、及び／又はＳ６８３ＷであるＢＧＬ１変異体。
- 請求項２に記載のＢＧＬ１変異体において、該変異体が（４）の置換を有する場合、更に配列番号３のＮ２６３、Ｋ３４５、Ｎ３６９、Ｇ３７２、Ｋ４２８、及び／又はＰ６６１、に対応する位置における１つ以上の置換を更に含むＢＧＬ１変異体。
- 請求項９に記載のＢＧＬ１変異体において、前記置換がＮ２６３Ｃ、Ｋ３４５Ｅ、Ｎ３６９Ｅ、Ｎ３６９Ｔ、Ｇ３７２Ａ、Ｋ４２８Ｎ、Ｐ６６１Ｅ、Ｐ６６１Ｌ、及び／又はＳ６８３ＷであるＢＧＬ１変異体。
- 請求項２に記載のＢＧＬ１変異体において、該変異体が（５）の置換を有する場合、更に配列番号３のＲ１７９、Ｎ２３８、Ｎ２６４、Ｎ２６５、Ｎ３６９、Ｄ３７０、及び／又はＫ６５６に対応する位置における１つ以上の置換を更に含むＢＧＬ１変異体。
- 請求項１１に記載のＢＧＬ１変異体において、前記置換がＲ１７９Ｖ、Ｎ２３８Ｆ、Ｎ２６４Ｍ、Ｎ２６５Ｍ、Ｎ３６９Ｉ、Ｄ３７０Ｗ、及び／又はＫ６５６ＲであるＢＧＬ１変異体。
- 請求項１に記載のＢＧＬ１変異体において、
（ｉ）置換がＩ５６７Ｑである場合、改良された活性が（ａ）および（ｂ）である、
（ｉｉ）置換がＩ５６７Ｖである場合、改良された活性が（ｂ）、（ｃ）および（ｅ）である、
（ｉｉｉ）置換がＩ５６７Ｅである場合、改良された活性が（ｂ）および（ｄ）である、
（ｉｖ）置換がＩ５６７Ｋである場合、改良された活性が（ｂ）および（ｃ）である、
（ｖ）置換がＩ５６７Ｒである場合、改良された活性が（ｂ）および（ｃ）である、
（ｖｉ）置換がＩ５６７Ｓである場合、改良された活性が（ａ）および（ｄ）である、
（ｖｉｉ）置換がＩ５６７Ｖである場合、改良された活性が（ｂ）、（ｃ）、および（ｅ）である、
（ｖｉｉｉ）置換がＩ５６７Ｙである場合、改良された活性が（ｅ）および（ｇ）である、変異体。 - 請求項１乃至４のいずれか１項に記載のＢＧＬ１変異体であって、前記ＢＧＬ１変異体が配列番号３に対応する位置における１つ以上の置換を含み、前記置換が
Ｑ２２６Ｆ；
Ｅ１７０Ｆ｜Ｑ２２６Ｙ｜Ｔ２４２Ｓ｜Ｓ３１２Ｙ｜Ｇ３７２Ａ｜Ｖ６０３Ｇ｜Ｐ６６１Ｆ｜Ｔ６６６Ｃ；
Ｑ２２６Ｙ｜Ｔ６６６Ｃ；
Ｅ１７０Ｆ｜Ｑ２２６Ｙ｜Ｎ３６９Ｙ｜Ｇ３７２Ａ｜Ｐ６６１Ｆ；
Ｅ１７０Ｆ｜Ｑ２２６Ｙ｜Ｎ３６９Ｙ｜Ｇ３７２Ａ；
Ｑ２２６Ｙ｜Ｔ２４２Ｓ｜Ｇ３７２Ａ｜Ｐ６６１Ｆ；
Ｅ１７０Ｆ；
Ｅ１７０Ｆ｜Ｖ６０３Ｇ；
Ｅ１７０Ｆ｜Ｓ３１２Ｙ｜Ｎ３６９Ｙ；
Ｅ１７０Ｆ｜Ｑ２２６Ｙ｜Ｎ３６９Ｙ｜Ｇ３７２Ａ｜Ｔ６６６Ｃ；
Ｅ１７０Ｆ｜Ｔ２４２Ｓ｜Ｎ３６９Ｙ｜Ｇ３７２Ａ｜Ｖ６０３Ｇ｜Ｔ６６６Ｃ；
Ｎ２６３Ｃ｜Ｋ３４５Ｅ｜Ｎ３６９Ｅ；
Ｎ２６３Ｃ｜Ｋ３４５Ｅ｜Ｎ３６９Ｔ｜Ｋ４２８Ｎ；
Ｎ２６３Ｃ｜Ｋ３４５Ｅ｜Ｎ３６９Ｅ｜Ｐ６６１Ｌ；
Ｎ２６３Ｃ｜Ｋ３４５Ｅ｜Ｎ３６９Ｅ｜Ｇ３７２Ａ；
Ｎ２６３Ｃ｜Ｋ３４５Ｅ｜Ｎ３６９Ｅ｜Ｇ３７２Ａ｜Ｐ６６１Ｅ；
Ｎ２６３Ｃ｜Ｋ３４５Ｅ｜Ｎ３６９Ｅ｜Ｇ３７２Ａ｜Ｋ４２８Ｎ｜Ｐ６６１Ｅ｜Ｓ６８３Ｗ；
Ｓ６８３Ｗ；
Ｎ３６９Ｅ｜Ｓ６８３Ｗ；
Ｋ４２８Ｎ｜Ｓ６８３Ｗ；
Ｎ２６３Ｃ｜Ｎ３６９Ｔ｜Ｓ６８３Ｗ；
Ｎ２６３Ｃ｜Ｐ６６１Ｌ｜Ｓ６８３Ｗ；
Ｋ３４５Ｅ｜Ｎ３６９Ｅ｜Ｓ６８３Ｗ；
Ｋ３４５Ｅ｜Ｐ６６１Ｅ｜Ｓ６８３Ｗ；
Ｋ３４５Ｅ｜Ｋ４２８Ｎ｜Ｓ６８３Ｗ；
Ｎ３６９Ｔ｜Ｐ６６１Ｅ｜Ｓ６８３Ｗ；
Ｎ３６９Ｔ｜Ｐ６６１Ｌ｜Ｓ６８３Ｗ；
Ｇ３７２Ａ｜Ｐ６６１Ｅ｜Ｓ６８３Ｗ；
Ｎ２６３Ｃ｜Ｎ３６９Ｔ｜Ｐ６６１Ｅ｜Ｓ６８３Ｗ；
Ｋ３４５Ｅ｜Ｎ３６９Ｅ｜Ｇ３７２Ａ｜Ｓ６８３Ｗ；
Ｋ３４５Ｅ｜Ｎ３６９Ｅ｜Ｋ４２８Ｎ｜Ｓ６８３Ｗ；
Ｋ３４５Ｅ｜Ｎ３６９Ｅ｜Ｐ６６１Ｅ｜Ｓ６８３Ｗ；
Ｎ３６９Ｔ｜Ｇ３７２Ａ｜Ｋ４２８Ｎ｜Ｓ６８３Ｗ；
Ｎ３６９Ｔ｜Ｇ３７２Ａ｜Ｐ６６１Ｌ｜Ｓ６８３Ｗ；
Ｋ３４５Ｅ｜Ｎ３６９Ｔ｜Ｇ３７２Ａ｜Ｐ６６１Ｅ｜Ｓ６８３Ｗ；
Ｎ２６３Ｃ｜Ｋ３４５Ｅ｜Ｎ３６９Ｔ｜Ｇ３７２Ａ｜Ｋ４２８Ｎ｜Ｐ６６１Ｅ｜Ｓ６８３Ｗ；
Ｎ２３８Ｆ；
Ｒ１７９Ｖ｜Ｎ２３８Ｆ｜Ｄ３７０Ｗ；
Ｒ１７９Ｖ｜Ｎ２３８Ｆ｜Ｋ６５６Ｒ；
Ｒ１７９Ｖ｜Ｎ２３８Ｆ｜Ｒ２６５Ｍ；
Ｎ２３８Ｆ｜Ｒ２６５Ｍ｜Ｎ３６９Ｉ；
Ｎ２３８Ｆ｜Ｎ２６４Ｍ｜Ｒ２６５Ｍ｜Ｎ３６９Ｉ；から選択される、ＢＧＬ１変異体。 - （ａ）、（ｂ）、（ｃ）、（ｄ）、（ｅ）、（ｆ）、および（ｇ）から選択される任意の２つ、任意の３つ、任意の４つ、任意の５つ、任意の６つ、又は７つ全ての活性が改善されている請求項１乃至２１および１４のいずれか一項に記載のＢＧＬ１変異体。
- 請求項１乃至１５のいずれか一項に記載のＢＧＬ１変異体を含む組成物。
- 前記ＢＧＬ１変異体を豊富に含む組成物である、請求項１６に記載の組成物。
- 請求項１乃至１５のいずれか一項に記載のＢＧＬ１変異体をコードする単離核酸。
- 請求項１８の核酸を含む発現ベクター。
- 前記単離された核酸が調節配列に制御可能に結合している、請求項１９の発現ベクター。
- 請求項１８の核酸を含む宿主細胞。
- 好適な条件下で請求項２１の宿主細胞を培養してＢＧＬ１変異体を生成する工程を含むＢＧＬ１変異体の生産方法。
- 請求項１乃至１５のいずれか一項に記載のＢＧＬ１変異体とバイオマスとを接触させる工程を含むバイオマスを糖へと変換させる方法。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26324009P | 2009-11-20 | 2009-11-20 | |
| US61/263,240 | 2009-11-20 | ||
| PCT/US2010/057531 WO2011063308A2 (en) | 2009-11-20 | 2010-11-19 | Beta-glucosidase i variants with improved properties |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015120937A Division JP2015211678A (ja) | 2009-11-20 | 2015-06-16 | 改良された特性を有するβ−グルコシダーゼＩ変異体 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013511280A JP2013511280A (ja) | 2013-04-04 |
| JP2013511280A5 true JP2013511280A5 (ja) | 2014-01-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2012540120A Pending JP2013511280A (ja) | 2009-11-20 | 2010-11-19 | 改良された特性を有するβ−グルコシダーゼＩ変異体 |
| JP2015120937A Withdrawn JP2015211678A (ja) | 2009-11-20 | 2015-06-16 | 改良された特性を有するβ−グルコシダーゼＩ変異体 |
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| JP2015120937A Withdrawn JP2015211678A (ja) | 2009-11-20 | 2015-06-16 | 改良された特性を有するβ−グルコシダーゼＩ変異体 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US9447400B2 (ja) |
| EP (6) | EP2599864B1 (ja) |
| JP (2) | JP2013511280A (ja) |
| CN (2) | CN105316303B (ja) |
| BR (1) | BR112012011711A2 (ja) |
| CA (1) | CA2780767A1 (ja) |
| DK (6) | DK2501806T3 (ja) |
| IN (1) | IN2012DN03825A (ja) |
| RU (1) | RU2012125624A (ja) |
| WO (1) | WO2011063308A2 (ja) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2010298308A1 (en) | 2009-09-23 | 2012-03-01 | Danisco Us Inc. | Novel glycosyl hydrolase enzymes and uses thereof |
| MX2012006016A (es) | 2009-11-25 | 2012-06-25 | Codexis Inc | Variantes de beta-glucosidasa recombinante para produccion de azucares solubles de biomasa celulosica. |
| WO2011079048A2 (en) | 2009-12-23 | 2011-06-30 | Danisco Us Inc. | Methods for improving the efficiency of simultaneous saccharification and fermentation reactions |
| EP2622069B1 (en) | 2010-10-01 | 2015-11-25 | Novozymes, Inc. | Beta-glucosidase variants and polynucleotides encoding same |
| KR20130132789A (ko) * | 2010-11-05 | 2013-12-05 | 아사히 가라스 가부시키가이샤 | 스키조사카로미세스속 효모의 형질 전환체 및 그 제조 방법 |
| DK2686434T3 (da) | 2011-03-17 | 2021-10-11 | Danisco Us Inc | Fremgangsmåde til reduktion af viskositet i saccharificeringsproces |
| CN102816748B (zh) * | 2011-06-09 | 2014-07-16 | 中国科学院上海生命科学研究院 | 一种新型β-葡萄糖苷酶及其编码基因和应用 |
| CA2892054A1 (en) | 2012-12-07 | 2014-06-12 | Ling Hua | Compositions and methods of use |
| ES2521940B1 (es) | 2013-05-10 | 2015-09-09 | Abengoa Bioenergía Nuevas Tecnologías, S.A. | Variantes de beta-glucosidasa con actividad de transglicosilación reducida |
| JP2016533741A (ja) | 2013-07-29 | 2016-11-04 | ダニスコ・ユーエス・インク | 酵素の変異体 |
| CN105745322A (zh) * | 2013-09-17 | 2016-07-06 | 易登尼有限公司 | 用于增加生物质糖化的改良的β-葡萄糖苷酶 |
| NZ722003A (en) * | 2014-02-26 | 2023-06-30 | Chr Hansen As | Variants of chymosin with improved milk-clotting properties |
| US10053671B2 (en) | 2014-06-20 | 2018-08-21 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
| AP2016009640A0 (en) * | 2014-08-08 | 2016-12-31 | Xyleco Inc | Aglycosylated enzyme and uses thereof |
| WO2016100837A1 (en) | 2014-12-18 | 2016-06-23 | Danisco Us Inc | Engineered multifunctional enzymes and methods of use |
| WO2016100825A1 (en) | 2014-12-18 | 2016-06-23 | Danisco Us Inc | Engineered multifunctional enzymes and methods of use |
| CN107429267B (zh) * | 2014-12-19 | 2021-07-16 | 丹尼斯科美国公司 | 葡糖淀粉酶共混物 |
| BR112017027729A2 (pt) | 2015-07-07 | 2018-09-11 | Danisco Us Inc | indução de expressão de gene com o uso de uma mistura de açúcar de concentração alta |
| CN108368530A (zh) * | 2015-12-10 | 2018-08-03 | 帝斯曼知识产权资产管理有限公司 | β-葡糖苷酶及其用途 |
| WO2017097896A1 (en) * | 2015-12-10 | 2017-06-15 | Dsm Ip Assets B.V. | Beta-glucosidase and uses thereof |
| CN108884481A (zh) * | 2015-12-10 | 2018-11-23 | 帝斯曼知识产权资产管理有限公司 | β-葡糖苷酶及其用途 |
| EP3336180A1 (en) | 2016-07-04 | 2018-06-20 | Evonik Degussa GmbH | Mutant alkb gene |
| WO2018053058A1 (en) | 2016-09-14 | 2018-03-22 | Danisco Us Inc. | Lignocellulosic biomass fermentation-based processes |
| KR102593668B1 (ko) | 2016-10-04 | 2023-10-24 | 다니스코 유에스 인크. | 유도 기질 부재 하의 사상 진균 세포에서의 단백질 생산 |
| WO2018106656A1 (en) | 2016-12-06 | 2018-06-14 | Danisco Us Inc | Truncated lpmo enzymes and use thereof |
| MX2019007335A (es) | 2016-12-21 | 2019-09-09 | Dupont Nutrition Biosci Aps | Métodos para usar serina proteasas termoestables. |
| EP3592429A1 (en) | 2017-03-06 | 2020-01-15 | DuPont Nutrition Biosciences ApS | Novel fungal fucosidases and their use in preventing and/or treating a pathogenic infection in an animal |
| AU2018268865A1 (en) * | 2017-05-16 | 2019-12-05 | The Curators Of The University Of Missouri | Immobilized enzyme complexes and related methods |
| WO2019074828A1 (en) | 2017-10-09 | 2019-04-18 | Danisco Us Inc | CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE |
| JP7051491B2 (ja) * | 2018-02-26 | 2022-04-11 | 花王株式会社 | 変異β－グルコシダーゼ |
| CA3093378A1 (en) | 2018-03-09 | 2019-09-12 | Danisco Us Inc | Glucoamylases and methods of use thereof |
| CA3105609A1 (en) | 2018-07-04 | 2020-01-09 | Danisco Us Inc | Glucoamylases and methods of use, thereof |
| KR20210094587A (ko) | 2018-11-20 | 2021-07-29 | 듀폰 뉴트리션 바이오사이언시즈 에이피에스 | 조작된 견고한 고 Tm-피타제 클레이드 폴리펩타이드 및 이의 단편 |
| US11241492B2 (en) * | 2019-01-23 | 2022-02-08 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to genes in influenza viruses |
| CN113874496A (zh) | 2019-02-08 | 2021-12-31 | 威斯康星校友研究基金会(Warf) | 人源化细胞系 |
| EP4022046A2 (en) | 2019-08-27 | 2022-07-06 | Wisconsin Alumni Research Foundation (WARF) | Recombinant influenza viruses with stabilized ha for replication in eggs |
| US20230240334A1 (en) | 2020-02-28 | 2023-08-03 | Dupont Nutrition Biosciences Aps | Feed compositions |
| CN115478060B (zh) * | 2021-06-16 | 2023-11-28 | 弈柯莱生物科技(集团)股份有限公司 | 一种糖基转移酶及其应用 |
| CN113842454A (zh) * | 2021-09-30 | 2021-12-28 | 中国人民解放军空军军医大学 | 一种基于vsv载体的htnv疫苗及其制备方法和应用 |
| CA3234874A1 (en) | 2021-10-15 | 2023-04-20 | Martijn Scheffers | Glucoamylase variants and methods for use thereof |
| WO2023092018A1 (en) | 2021-11-17 | 2023-05-25 | Dupont Nutrition Biosciences Aps | Improved enzymatic modification of galactolipids in food |
| CN116426506B (zh) * | 2023-03-10 | 2024-01-30 | 云南师范大学 | 低温活性提高的β-木糖苷酶突变体D259G及其应用 |
Family Cites Families (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4822516A (en) | 1986-12-08 | 1989-04-18 | Kao Corporation | Detergent composition for clothing incorporating a cellulase |
| EP0406314B1 (en) | 1988-03-24 | 1993-12-01 | Novo Nordisk A/S | A cellulase preparation |
| US5776757A (en) | 1988-03-24 | 1998-07-07 | Novo Nordisk A/S | Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof |
| DK494089D0 (ja) | 1989-10-06 | 1989-10-06 | Novo Nordisk As | |
| US5326477A (en) | 1990-05-07 | 1994-07-05 | Bio-Sep, Inc. | Process for digesting solid waste |
| US5246853A (en) | 1990-10-05 | 1993-09-21 | Genencor International, Inc. | Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I |
| US5475101A (en) | 1990-10-05 | 1995-12-12 | Genencor International, Inc. | DNA sequence encoding endoglucanase III cellulase |
| WO1992006209A1 (en) | 1990-10-05 | 1992-04-16 | Genencor International Inc. | Trichoderma reesei containing deleted and/or enriched cellulase and other enzyme genes and cellulase compositions derived therefrom |
| DE69133612D1 (de) | 1990-12-10 | 2009-04-02 | Genencor Int | Verbesserte Saccharifizierung von Zellulose durch Klonierung und Vervielfältigung des beta-Glukosidase Genes aus Trichoderma |
| US6103464A (en) * | 1990-12-10 | 2000-08-15 | Genencor International, Inc. | Method of detecting DNA encoding a β-glucosidase from a filamentous fungus |
| US6021536A (en) | 1993-03-04 | 2000-02-08 | Wasinger; Eric | Mechanical desizing and abrading device |
| FI932521A0 (fi) | 1993-06-02 | 1993-06-02 | Alko Ab Oy | Nytt endoglukanasenzym |
| US6426200B1 (en) | 1994-09-15 | 2002-07-30 | University Of Georgia Research Foundation, Inc. | Methods for enzymatic deinking of waste paper |
| JP3307947B2 (ja) | 1995-01-26 | 2002-07-29 | ノボザイムス アクティーゼルスカブ | キシラナーゼを含有する動物飼料添加剤 |
| WO1997004160A1 (en) | 1995-07-19 | 1997-02-06 | Novo Nordisk A/S | Treatment of fabrics |
| TR199800866T2 (xx) | 1995-11-15 | 2001-03-21 | Novozymes A/S | Boyanmış denimin yapışkanının giderilmesi ve taşlanması yöntemi |
| CN1209838A (zh) | 1996-01-29 | 1999-03-03 | 诺沃挪第克公司 | 纤维素织物的脱浆方法 |
| US6254722B1 (en) | 1996-03-27 | 2001-07-03 | North Carolina State University | Method for making dissolving pulp from paper products containing hardwood fibers |
| US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| US6069122A (en) | 1997-06-16 | 2000-05-30 | The Procter & Gamble Company | Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution |
| US6265204B1 (en) | 1997-01-17 | 2001-07-24 | Genencor International, Inc. | DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi |
| BR9807941A (pt) | 1997-04-07 | 2000-02-22 | Unilever Nv | Processos para a produção e para cultivar um bolor transformado, e, bolor transformado |
| WO1998056958A1 (en) | 1997-06-10 | 1998-12-17 | Xyrofin Oy | Process for the production of xylose from a paper-grade, hardwood pulp |
| ATE234913T1 (de) | 1997-11-10 | 2003-04-15 | Procter & Gamble | Verfahren zur herstellung einer waschmitteltablette |
| US6187580B1 (en) | 1997-11-24 | 2001-02-13 | Novo Nordisk A/S | Pectate lyases |
| EP1082482A1 (en) | 1998-05-01 | 2001-03-14 | Novozymes A/S | Enhancers such as n-hydroxyacetanilide |
| DE19824705A1 (de) | 1998-06-03 | 1999-12-09 | Henkel Kgaa | Amylase und Protease enthaltende Wasch- und Reinigungsmittel |
| US5980581A (en) | 1998-09-08 | 1999-11-09 | The Virkler Company | Process for desizing and cleaning woven fabrics and garments |
| US6024766A (en) | 1999-01-27 | 2000-02-15 | Wasinger; Eric M. | Process for enzymatic desizing of garments and enzyme deactivation |
| US6982159B2 (en) | 2001-09-21 | 2006-01-03 | Genencor International, Inc. | Trichoderma β-glucosidase |
| US7045332B2 (en) | 2001-12-18 | 2006-05-16 | Genencor International, Inc. | BGL4 β-glucosidase and nucleic acids encoding the same |
| US7005289B2 (en) | 2001-12-18 | 2006-02-28 | Genencor International, Inc. | BGL5 β-glucosidase and nucleic acids encoding the same |
| WO2004099228A2 (en) * | 2003-05-02 | 2004-11-18 | Novozymes Inc. | Variants of beta-glucosidases |
| US20060041961A1 (en) | 2004-03-25 | 2006-02-23 | Abad Mark S | Genes and uses for pant improvement |
| US7413887B2 (en) | 2004-05-27 | 2008-08-19 | Genecor International, Inc. | Trichoderma reesei glucoamylase and homologs thereof |
| JP5149785B2 (ja) | 2005-04-12 | 2013-02-20 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | エタノールを得るためのバイオマスの処理 |
| ES2368165T3 (es) * | 2005-08-04 | 2011-11-15 | Novozymes, Inc. | Polipéptidos con actividad de beta-glucosidasa y polinucleótidos que codifican los mismos. |
| US7923236B2 (en) * | 2007-08-02 | 2011-04-12 | Dyadic International (Usa), Inc. | Fungal enzymes |
| MX2010002474A (es) | 2007-09-07 | 2010-03-29 | Danisco Us Inc | Composiciones de celulasa completa fungica filamentosa mejoradas de beta-glucosidasa y metodos de uso. |
| EP2479265B1 (en) | 2007-10-09 | 2014-06-11 | Danisco US Inc. | Glucoamylase variants with altered properties |
| CA2716806A1 (en) | 2008-02-29 | 2009-09-03 | University Of Central Florida Research Foundation, Inc. | Production and use of plant degrading materials |
| CN102482656A (zh) * | 2008-08-29 | 2012-05-30 | 埃欧金能源公司 | 具有提高的稳定性的经修饰的β-糖苷酶 |
| FR2935986B1 (fr) * | 2008-09-12 | 2012-11-23 | Inst Francais Du Petrole | Variants de beta-glucosidase a activite amelioree et leurs utilisations. |
| WO2010135836A1 (en) * | 2009-05-29 | 2010-12-02 | Iogen Energy Corporation | Novel beta-glucosidase enzymes |
| KR20140027154A (ko) | 2011-03-17 | 2014-03-06 | 다니스코 유에스 인크. | 글리코실 가수분해효소 및 바이오매스 가수분해를 위한 그의 용도 |
| DK2686434T3 (da) | 2011-03-17 | 2021-10-11 | Danisco Us Inc | Fremgangsmåde til reduktion af viskositet i saccharificeringsproces |
| US8468683B2 (en) * | 2011-10-31 | 2013-06-25 | HGST Netherlands B.V. | High bevel angle magnetic writer pole fabrication process |
| US20150252343A1 (en) | 2012-10-31 | 2015-09-10 | Danisco Us Inc. | Beta-glucosidase from magnaporthe grisea |
| JP2015533293A (ja) | 2012-10-31 | 2015-11-24 | ダニスコ・ユーエス・インク | 組成物及び使用方法 |
| EP2914718A1 (en) | 2012-10-31 | 2015-09-09 | Danisco US Inc. | Beta-glucosidase from neurospora crassa |
-
2010
- 2010-11-19 EP EP12199332.3A patent/EP2599864B1/en not_active Not-in-force
- 2010-11-19 EP EP10782511.9A patent/EP2501806B1/en not_active Not-in-force
- 2010-11-19 DK DK10782511.9T patent/DK2501806T3/da active
- 2010-11-19 DK DK12199329.9T patent/DK2599862T3/da active
- 2010-11-19 EP EP12199328.1A patent/EP2602317B1/en not_active Not-in-force
- 2010-11-19 CN CN201510672711.8A patent/CN105316303B/zh not_active Expired - Fee Related
- 2010-11-19 EP EP12199327.3A patent/EP2599861B1/en not_active Not-in-force
- 2010-11-19 CA CA2780767A patent/CA2780767A1/en not_active Abandoned
- 2010-11-19 BR BR112012011711A patent/BR112012011711A2/pt not_active IP Right Cessation
- 2010-11-19 DK DK12199330.7T patent/DK2599863T3/da active
- 2010-11-19 DK DK12199328.1T patent/DK2602317T3/da active
- 2010-11-19 DK DK12199327.3T patent/DK2599861T3/da active
- 2010-11-19 EP EP12199330.7A patent/EP2599863B1/en not_active Not-in-force
- 2010-11-19 IN IN3825DEN2012 patent/IN2012DN03825A/en unknown
- 2010-11-19 US US13/510,902 patent/US9447400B2/en not_active Expired - Fee Related
- 2010-11-19 WO PCT/US2010/057531 patent/WO2011063308A2/en active Application Filing
- 2010-11-19 EP EP12199329.9A patent/EP2599862B1/en not_active Not-in-force
- 2010-11-19 DK DK12199332.3T patent/DK2599864T3/da active
- 2010-11-19 CN CN201080061975.5A patent/CN102844429B/zh not_active Expired - Fee Related
- 2010-11-19 RU RU2012125624/10A patent/RU2012125624A/ru not_active Application Discontinuation
- 2010-11-19 JP JP2012540120A patent/JP2013511280A/ja active Pending
-
2015
- 2015-06-16 JP JP2015120937A patent/JP2015211678A/ja not_active Withdrawn
-
2016
- 2016-08-29 US US15/250,626 patent/US20170145396A1/en not_active Abandoned
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