JP2012193992A - Object substance collection device and collection method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a collection device capable of collecting each of gas and substances in the outer atmosphere which are object substances released from e.g. a skin surface as a sample.SOLUTION: A collection device is provided where a sheet to collect gas released from a skin surface and a sheet to collect air in the environment are bonded together such that each collection surface faces each other.

Description

  The present invention relates to an apparatus for collecting a target substance released from a sample and a collection method using the apparatus.

  In recent years, it has been suggested that the state of health can be measured from the concentration of gas components released from the human skin surface. For example, a correlation between the concentration of acetone in a gas component released from the skin and the concentration of β-hydroxybutyric acid in blood has been reported (Non-Patent Document 1). According to this, unlike blood collection, it is possible to determine the health state from a sample collected non-invasively with respect to the subject.

  Patent Document 1 describes a method of collecting gas released from the human skin surface, and a gas collection container has been devised. Patent Document 2 discloses a gas adsorption sheet. These all facilitate introduction into the analyzer by transferring the gas released from the skin to a container or sheet.

Japanese Patent No. 3850662 JP 2006-214747 A

Kazutoshi Nose, Takaharu Kondo, Shuki Araki, Takao Tsuda: Analytical Chemistry: Vol. 54, p. 161 (2005)

  In order to improve the accuracy of measurement, it is important to distinguish between the target substance released from the skin surface and the same kind of substance contained in the air. The present invention provides a target substance collecting device that collects a target substance in a state where the same kind of gas contained in the air is not mixed with the gas released from the skin surface.

According to the present invention,
A first member having a collection part for collecting a target substance released from the sample by contacting a part of the sample, and a substance in the space by being exposed to the space to which the sample is exposed And a second member having a collecting part for collecting
Holding means for holding the first member and the second member in an overlapping manner so that the collection portion of the first member and the collection portion of the second member are both shielded from outside air and held. There is provided a target substance collecting device characterized by comprising:

  According to the present invention, the target substance released from the sample can be collected without touching the outside, for example, air, by contacting the collection part of the first member to a part of the sample. Furthermore, since the collection part of the second member is exposed to the outside world at the same timing as when the first member is used, collection of substances in the outside world, for example, a reference gas can be started simultaneously.

It is a schematic diagram which shows the structure of the target substance collection apparatus which concerns on 1st Embodiment. It is a schematic diagram which shows the time of use of the target substance collection apparatus which concerns on 1st Embodiment. It is the schematic diagram which looked at the 1st member of the target substance collection device concerning a 1st embodiment from the collection part. It is a schematic diagram which shows the target substance collection apparatus which concerns on 2nd Embodiment. It is a schematic diagram which shows the target substance collection apparatus which concerns on 3rd Embodiment.

(First embodiment)
The target substance collection device according to the first embodiment is a form in which a first member and a second member are pasted with their collection parts facing each other.

  FIG. 1 is a schematic diagram showing the configuration of the target substance collection device according to the first embodiment.

  The first member includes a cover sheet 101, a collection sheet 103 that is a collection unit, and a protection sheet 107. The second member includes a cover sheet 102, a collection sheet 104 that is a collection unit, and a protection sheet 108.

  The first member is a member that sticks to the surface of a sample such as skin, and the second member is a reference member.

  The collection sheet 103 is disposed so as to face the skin, and takes in a target gas (target substance) released from the skin via the protective sheet 107. The gas released from the skin contains the target gas.

  The cover sheet 101, the collection sheet 103, and the protection sheet 107 are all flexible. Thereby, it can respond to the sample which bends, such as an elbow and a knee.

  The cover sheet 105 that is a holding means is adhesive, and its surface (adhesive surface) sticks to the sample surface and does not peel off at least during collection. The collection sheet 103 has a non-woven fabric or filter paper as a base material. Silica gel is provided there, and it collects and changes color by touching acetone (substance) released from the skin. Silica gel has a solvatochromic material. The solvatochromic material is, for example, a Reichardt's dye such as (2,6-diphenyl-4- (2,4,6-triphenylpyridino) phenolate) or a nickel complex such as [Ni (acac) (tmen)] BPh4. .

  The cover sheet 101 is a sheet that does not transmit gas, and the material thereof is polyvinyl fluoride.

  The protective sheet 107 is a sheet for preventing dirt on the skin from directly touching the collection sheet 103.

  As illustrated in FIG. 1, the collecting portion of the first member and the second member is shielded from the outside air by the first member and the second member being bonded together. Thereby, the collection sheet 103 is protected until acetone is collected from the skin.

  The second member has the same configuration as the first member. Among these, the collection sheet 104 of the second member has the same configuration as the collection sheet 103 of the first member. The first member and the second member are peeled off from the pasted state, the first member is stuck to the skin, and at the same time, the second member is not stuck to the skin and is exposed to the outside air. Accordingly, while the first member collects acetone, which is a substance in the space, the second member takes in acetone in the outside air and changes its color.

  While the collection sheet 103 of the first member is collecting acetone released from the skin, the collection sheet 103 is not exposed to the outside air. Therefore, the degree of change of the collection sheet 103 is affected by the amount of acetone released from the skin.

  On the other hand, the reason why acetone contained in the outside air is collected by the collecting sheet 104 is to use the acetone.

  Specifically, it is separation of the amount of acetone derived from the skin and the amount of acetone in the atmosphere out of the total amount of acetone collected in the collection sheet 103. The collection sheet 103 collects both acetone derived from the outside world and acetone released from the skin. On the other hand, only the external acetone is collected on the collection sheet 104. Since the collection sheets 103 and 104 are exposed to the outside world at the same timing, it is possible to accurately grasp the amount of acetone released from the skin by taking the difference between the two. The amount of acetone is, for example, the concentration.

  The difference between the two will be described. The difference is the difference between the degree of discoloration of the collection sheet 103 and that of the collection sheet 104. A plurality of references having different color levels depending on the density are prepared in advance. The reference corresponds to the degree of color and density. If such a reference is used, the reference closest to the degree of discoloration of the respective collection sheets 103 and 104 can be selected, and the difference between the densities can be calculated.

  The cover sheets of the first and second members of the target substance collection device according to the present embodiment do not transmit the target gas, but in the present invention, the cover sheet uses another gas other than the target gas. It may be transparent.

  Although both the first member and the second member according to the present embodiment are flexible, at least one of the cover sheets is difficult to bend, specifically, a cover sheet such as a metal plate that does not bend. It may be.

  The sample according to the present embodiment is skin in a mammalian part such as an extremity or a back, but the sample according to the present invention may be an internal organ other than the skin. The sample may be a fish, a plant or the like.

  The target substance collected by the first member according to the present embodiment is a gas (gas), but the target substance according to the present invention may be an individual or a liquid. Preferably, the gas is easily mixed with outside air.

  In addition to acetone, the gas may be alcohols, aldehydes, isoprene, nitrogen oxides, or the like. Acetone is preferred. This is because there is a correlation between the concentration of ketone bodies in the blood and the concentration of acetone in the gas collected from the skin.

  In the first member according to the present embodiment, the cover sheet has adhesiveness, but in the present invention, if the first member or the second member is held in a stacked state, the one that enables it is held. Means. In addition, for example, at least one of the first member and the second member may have adhesiveness, and neither member has adhesiveness, and a member having adhesiveness on both sides, for example, is provided. It may be used. Specifically, a member having adhesiveness on both surfaces may be disposed on a surface where both members are opposed to each other. Further, both members may be bonded with scissors such as a clamp as in a third embodiment described later.

  Although the collection sheet 103 according to the present embodiment includes silica gel, the collection sheet may be a molecular sieve instead.

  These are preferred when collecting acetone. In addition, an adsorbing porous material, a non-porous material that collects acetone by an interaction force, an amine compound that specifically adsorbs acetone, and the like can be used. Similarly, the collection sheet 104 may have one selected from these. The collection sheets 103 and 104 may have the same thing or may have different things.

  When using a substance that collects the target substance such as silica gel, molecular sieves, other porous materials, non-porous materials, or amine compounds, the target substance may be recovered by applying heat after collection, and the concentration may be measured. In this case, the concentration of the collected target substance can be measured using, for example, gas chromatography. In the case of using gas chromatography, an accurate concentration measurement can be performed by preparing a calibration curve in advance using a target substance having a known concentration.

  Both the first member and the second member according to the present embodiment have the protective sheets 107 and 108, respectively, but at least one of them may be omitted. For example, if at least one of the cover sheets 101 and 102 has adhesiveness, at least one of the protective sheets 107 and 108 may be omitted. Alternatively, at least one of the cover sheets 101 and 102 may be provided with at least one of the protective sheets 107 and 108 having an adhesive property.

  FIG. 2 is a schematic diagram illustrating the use of the target substance collection device according to the first embodiment. Specifically, it is a schematic view of a state in which skin gas is collected using the gas collection sheet shown in FIG.

  The first member 201 is attached to a part of the skin 203 (part of the sample), and the second member 202 is allowed to stand so that the collection sheet 104 is exposed to the outside air of the skin.

  Drawing 3 is a mimetic diagram which looked at the 1st member from the collection part of the target substance collection device concerning a 1st embodiment. The protective sheet 107 is disposed outside the collection sheet 103 and has an edge of the cover sheet 101 on the outside. The protective sheet 107 is wider than the collection sheet 103 and protects the surface of the collection sheet 103. The collection sheet 103 is sandwiched between the protection sheet 107 and the cover sheet 101. When the collection sheet 103 contains a powdery substance, it is not scattered by the protective sheet 107.

(Second Embodiment)
In the target substance collection device according to the second embodiment of the present invention, the collection sheets 103 and 104 are less than half the area of the cover sheets 101 and 102, and each is close to one side of the cover sheet 101.
When the first member and the second member are bonded together, the collection sheets are shifted without facing each other. Others are the same as in the first embodiment.

(Third embodiment)
The target substance collection device according to the second embodiment of the present invention is a form in which the first and second members are bonded together with scissors such as a clamp. Other than that, it is the same as either the first or second embodiment.

  For example, gas released from the skin can be collected by the gas collection sheet described in the present invention.

103, 104 Collection part 105 Holding means 201 First member 202 Second member

Claims (7)

A first member having a collection part for collecting a target substance released from the sample by contacting a part of the sample, and a substance in the space by being exposed to the space to which the sample is exposed And a second member having a collecting part for collecting
Holding means for holding the first member and the second member in an overlapping manner so that the collection portion of the first member and the collection portion of the second member are both shielded from outside air and held. The target substance collection apparatus characterized by having.   The target substance collecting apparatus according to claim 1, wherein each of the first member and the second member is a sheet.   The target substance collection device according to claim 1, wherein the substance in the space is the target substance.   The target substance collection device according to any one of claims 1 to 3, wherein the target substance is acetone.   5. The target substance collecting apparatus according to claim 1, wherein the holding unit is an adhesive surface included in at least one of the first member and the second member.   5. The target substance collecting apparatus according to claim 1, wherein the holding unit is a unit that sandwiches the first member and the second member. 6. The target material collection device in which the collection part of the first member and the collection part of the second member are blocked from the outside by overlapping the first member and the second member A collection method for collecting the target substance by:
Peel off the first member and the second member that overlap each other,
The collection part of the first member is in contact with a part of the sample,
Exposing the collection part of the second member to the space where the sample is exposed;
Collecting the target substance released from the sample by the collecting part of the first member;
The collection method which collects the substance in the said space by the collection part of a said 2nd member.

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