JP2012095630A - Parallel cultivation system for aquatic animal and plant by using microorganism activated with micro-nanobubble - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a system for converting ammoniacal materials excreted by aquatic animals to harmless nitrates by using microorganisms.SOLUTION: Ammonia oxidizing bacteria (aerobic bacteria) fixed to Kougaseki (Niijima-feldspar stone), etc., are activated by supplying micro-nanobubbles from below a net-type biological filter 1 and a box-type biological filter 2, and ammonia components in a water tank 3 are oxidized to nitrous acid by the action of the bacteria.

Description

  The present invention relates to an effective improvement of a system for purifying a particularly toxic ammonia-based component of excrement of aquatic animals cultivated in an aquarium into harmless nitrate using microorganisms. The present invention also relates to an aquatic animal capable of performing hydroponic cultivation of plants such as vegetables and the like, and a system for parallel cultivation of plants by using a component nitrated earlier as a fertilizer and circulating water in the aquarium. One of the most important things to be aware of when aquatic animals are cultivated or hydroponically is the occurrence of disease. The present invention relates to a system for suppressing the occurrence of diseases in the parallel cultivation.

  In recent years, it has become very difficult to secure aquatic animals as resources due to problems such as marine pollution, fishing rights, and environmental protection. As a method for solving this problem, Patent Document 1 proposes a system in which aquatic animals can be cultivated on land by eliminating the need for water change by purifying seawater with microorganisms.

  Further, the same patent document describes a method for cultivating a plant at the same time using nitrate from the excrement of aquatic animals to be cultured and using it as a fertilizer.

  In Patent Document 2, in order to efficiently purify an aquatic animal habitat contaminated water area or an aquarium containing natural water, micro-nano bubbles, which are fine bubbles in water, are brought into contact with microorganisms living in natural water areas. A method is disclosed in which the nitrification of ammonia and the like is accelerated by activating the nitrile.

JP2009-022282

JP 2006;

  In Patent Document 1, by performing the method described, aquatic animal farming inland that could not be put into practical use has been made possible by nitrifying ammonia and the like excreted by aquatic animals efficiently into nitrite and nitrate. A method for enabling is disclosed. Furthermore, a proposal was made to improve profitability when commercialized by using nitrate converted from excrement of aquatic animals as a fertilizer for hydroponics and growing plants at the same time as aquaculture. Has been.

  In order to further improve the profitability of this project, it is desirable to increase the aquatic animals cultivated in the same size tank and increase the yield of plants to be cultivated. The more aquatic animals that are cultivated, the more excreta can grow, and the nitrate that can be nitrified increases, so the yield of plants hydroponically grown using it as a fertilizer increases, and the profitability of the project can be expected to improve. .

  However, if more aquatic animals are cultivated, more ammonia will be excreted and nitrification by microorganisms may not be able to catch up. To increase nitrification efficiency, the number of nitrifying bacteria should be increased. However, the number of bacteria is determined by the environment, so it is not necessary to simply introduce nitrifying bacteria. It is necessary to plan. Therefore, in the prior art, it was necessary to secure a wide area of bacteria.

Patent Document 2 describes a method of activating nitrifying bacteria with microbubbles.
However, there was a problem that even if it was actually performed in this way, there were few opportunities for microbubbles to come into contact with nitrifying bacteria, and the effect did not increase.

  The first technical problem of the present invention lies in how to grow and activate nitrifying bacteria in an aquaculture tank of a limited size.

  In addition, it is necessary to prevent infectious diseases from occurring in aquatic animals to be cultivated and to prevent the effects of infectious diseases on hydroponically cultivated plants. Aquaculture in a narrow area such as an aquarium can be described as a fight between fish stress and infection. It is also necessary to solve this problem in order to increase profitability as a business that is the object of the present invention.

The inventor of the present patent application has been studying lactic acid bacteria for many years, and when lactic acid bacteria given to aquatic animals as feed remain in the aquarium and settle, no harmful germs are generated, and not only farmed animals but also hydroponically grown plants I found that it has a tremendous effect on suppressing the disease.
Therefore, if not only feeding lactic acid bacteria with food but also establishing a method for generating and fixing them in the aquarium, the health of farmed animals can be protected, and administration of antibiotics and the like can be made unnecessary.
The second technical problem of the present invention is to propagate and activate lactic acid bacteria in a water tank.

In addition, as a means for improving profitability as a business, Patent Document 1 discloses a method of performing aquatic animal culture and hydroponic cultivation of plants in the same water tank.
When aquatic plants and aquatic animals are cultivated in the same aquarium, if the roots of the plants extend into the aquarium, the aquatic animals to be cultivated may bite or break the roots. However, separating the aquaculture tank and the cultivation tank requires extra space.
The third technical problem of the present invention is to put it into practical use as a highly profitable business by performing aquatic animal farming and plant cultivation in parallel in as little space as possible.

  By solving these technical problems and improving profitability as a business, for example, it is possible to develop aquaculture business even in spaces such as fallow land in a depopulated mountainous area or abandoned school ruins. The inventor's purpose is to contribute as much as possible to the solution of the conversion problem.

  As a water purification system using microorganisms, a net-type biofiltration device and a box-type biofiltration device invented by the present applicant are effective.

  A net-type biofiltration device is a unit in which a porous material that easily settles bacteria, such as coga stone, is stored in a net and an air diffuser pipe that sends air below the net can be arranged. By sending air from under the net, the ammonia-oxidizing bacteria (aerobic bacteria) that have settled on the corrugated stone are activated, and the ammonia component in the water tank is oxidized and converted into nitrous acid by its action.

  At the same time, nitrite-oxidizing bacteria (aerobic bacteria) that have settled on corrugated stones are also activated, and the nitrite component in the water tank is oxidized and converted into nitrate by its action. Although these two actions can be performed in parallel in the net filtration device, a dedicated net-type biofiltration device for oxidizing the nitrite component and converting it into nitrate may be provided in the water tank.

Moreover, the biofiltration apparatus of another method can also be comprised as follows.
A low-height hollow three-dimensional object is formed of a porous or fine net-like material that can permeate water, and the lower end of the air pump is connected through the hollow portion. This is called a bottom filter.

  The air pump places an air diffuser in the lower part of a cylinder arranged from below to above in the water tank, and the water in the cylinder also rises as the air discharged from the air diffuser rises in the form of bubbles in the cylinder. Raise. The rising air bubbles serve as a pump that pumps the water sucked from the lower part of the cylinder to the upper part of the cylinder.

A box in which the bottom filter is inserted is disposed in the filtration water tank, and a corrugated stone in which bacteria are fixed is disposed on the bottom filter. By pumping out the water inside the bottom filter with an air pump, a falling water flow can be generated around the coga stone in the box, and filtration by bacteria can be performed.
A device in which this system is integrated is called a box-type biofiltration device.

  The feature of the present invention resides in that micro / nano bubbles, which are fine underwater bubbles, are added to the water in the net-type biofiltration apparatus and the box-type biofiltration apparatus.

  In recent years, the application of fine air bubbles has been actively researched and used. These are so-called micro bubbles and nano bubbles. Microbubbles and nanobubbles are distinguished by the size of their bubbles (bubbles), but in the literature related to this patent application, microbubbles are bubbles having a diameter of 50 μm or less. Nano bubbles are bubbles with a diameter of 1 μm or less. Micro / nano bubbles are defined as a general term for micro bubbles and nano bubbles. This definition is commonly used by researchers in this field.

  Micro-nano bubbles have been used in various products in recent years by applying the appearance of unique properties depending on the bubble size. In particular, nano-bubbles are still being researched in various fields. The size of the bubbles is not constant and changes with time. For example, air bubbles in water tend to shrink with time.

  An apparatus for generating micro / nano bubbles is referred to as a micro / nano bubble generating apparatus. Although there is a method of finely cutting bubbles with a blade rotating in water, a method of sending air bubbles into water through a thin curtain with fine holes is well known unless it is used in a large amount as in the present invention. Uniform micro / nano bubbles can be generated by the size of the fine holes and the pressure of pushing out the air.

  Microbubbles float in the water without rising immediately because they are fine, and as time passes, they are absorbed into the water and the bubbles become smaller. Some of them dissolve in the water and disappear. It looks as if a shark drifts in the water. On the other hand, nanobubbles are difficult to see even if they are present in water because of their smaller bubbles.

  The net-type biofiltration device and the box-type biofiltration device are effective for nitrifying ammonia. However, when micro / nanobubbles are brought into contact with koga stones in which bacteria have settled in water, the bacteria are activated and innovative. It was found that the nitrification speed was increased.

  However, the presence of micro-nano bubbles floating in the water does not improve the effect because the contact with bacteria is not sufficient. When micro-nano bubbles are added to the water flow inside the net-type biofiltration device or the box-type biofiltration device according to the research of the patent inventor of the present application, the micro-nanobubbles are generated by the strong ascending or descending water flow in combination with the conventional diffuser. It was observed that the nitrification effect of ammonia was dramatically improved.

  By adopting this method, it has become possible to cultivate a greater number of aquatic animals in a tank of the same size as before.

  Therefore, a large amount of ammonia is excreted by farm animals, and a large amount of nitrate is generated when it is nitrified. Therefore, if plants are grown using the nitrate as a fertilizer, the yield increases.

It was also found that micro / nano bubbles are effective not only for nitrifying bacteria in the biofiltration apparatus but also for activating lactic acid bacteria.
This was discovered by the inventor when the aquatic animal feed was cultivated with lactic acid bacteria mixed in, and it was discovered that the occurrence of disease in the aquaculture tank could be suppressed. In order to do this, I was researching a method for effectively cultivating lactic acid bacteria. Among them, lactic acid bacteria were activated by adding lactic acid bacteria into the water tank and adding micro / nano bubbles, and the effect could be greatly enhanced.

  In addition, when mixed culture is carried out with saccharifying bacteria instead of lactic acid bacteria alone, the effect is further improved. The main metabolite of saccharifying bacteria is amylase, which works to promote the growth of lactic acid bacteria. When lactic acid bacteria are cultivated alone in a medium composed of nutrients mainly composed of starch, if lactic acid bacteria and saccharifying bacteria are mixed and cultured while the lactic acid bacteria grow about 10 times, the lactic acid bacteria will be about It proliferates about 100 times, and it is proved that the symbiotic relationship is materialized between both bacteria. It is also known that Bacillus NATTO, which is used when making natto, is a kind of saccharifying bacterium, but when lactic acid bacterium and natto bacterium are put into a water tank and experimented, the expected effect can be obtained. It was.

  The present invention proposes a method for suppressing the infectious disease of farm animals and increasing the yield by adding lactic acid bacteria and saccharifying bacteria in a water tank and adding micro / nano bubbles in the water tank.

  Separate aquatic animal farming and plant aquariums take up space. In order to enable aquaculture in a small space, it is desirable to cultivate plants in the upper part of an aquarium for aquatic animals. To achieve this, it is necessary to create an environment where plants can grow even if the roots do not extend into the aquarium.

  A promising way to achieve this is to lay a hydrogel film on the aquaculture tank and cultivate plants on it. Hydrogel films absorb moisture but do not permeate it, and do not permeate plant roots. Therefore, when a hydrogel film is placed on the water surface of the aquarium and planted on the top, the roots stick to the surface of the hydrogel film and absorb the moisture in the gel. Nitrate, which is a nutrient for plants, is absorbed into the gel, but viruses and bacteria cannot enter.

Depending on the plant to be cultivated, the supply of nitrogen fertilizer (nitrate) may be insufficient, in which case trace elements and nutrient deficiencies are supplied from the outside.
For this purpose, a trace element supply tank is prepared outside, and trace elements and insufficient nutrients are inserted therein. Trace elements and deficient nutrients are supplied from the tank to the roots of the plants to be grown through a trace element supply tube. The trace element supply tube uses a root-penetrating permeation tube made of fine mesh fibers or a hydrogel tube formed in a ring shape. They are permeable to moisture and nutrients, but prevent roots from entering the middle. By entwining the root of the plant in this tube, the plant can absorb trace elements and nutrient deficiencies.

  However, if a root shield penetration tube is used as a trace element supply tube, the substance to be supplied may hang on the hydrogel film, and depending on the form of trace elements and nutrient deficiencies (for example, components that are not polymerized) There is a possibility of passing through the hydrogel film. Depending on its components, it may have a negative effect on farmed animals. In order to prevent this, a permeation prevention tray is provided so that trace elements and insufficient nutrients leaked from the root penetration permeation tube do not spill on the hydrogel film. There is no such concern when using hydrogel tubes.

  By using a net-type biofiltration device and a box-type biofiltration device as a water purification system using microorganisms, micro-nano bubbles are generated in water, nitrifying bacteria are activated by the bubbles, and nitrification of ammonia is promoted. Since nitrification efficiency can be increased, farm animals can be increased in the aquarium.

  In addition, the amount of ammonia excreted by increasing the number of aquaculture organisms increases, and it becomes possible to increase the yield of plants grown using the increased nitrate as a fertilizer by nitrifying it. The yield of hydroponically cultivated plants using it as a fertilizer will increase, and the profitability of the project can be expected to improve.

  In addition, since micro / nano bubbles have the effect of activating lactic acid bacteria in water and promoting their growth, infection of aquatic animals and cultivated plants can be prevented. Furthermore, by utilizing the symbiosis of lactic acid bacteria and saccharifying bacteria, lactic acid bacteria can be proliferated and the effect of preventing infectious diseases can be enhanced. This leads to an increase in profitability as a business, which is the object of the present invention.

  Also, even if aquaculture plants and aquatic animals are cultivated in the same aquarium, the use of hydrogel film does not cause the roots of the plants to extend into the aquarium. And there is an effect that the space can be reduced.

  In addition, the hydrogel film laid on the aquaculture tank absorbs water and nitrate, which is a nutrient for the plant, but it cannot infect bacteria as well as bacteria so that the plant is not affected by infection.

  By integrating the above effects, it is possible to improve profitability as a business compared to conventional aquatic animal farming. For example, it is possible to develop aquaculture business even in a small space in a mountainous depopulated area. It can contribute to solving problems and depopulation problems.

  Since the roots of plants are said to be nitrifying and absorbing organs, in the case of freshwater fish farming, the roots can be put directly into the liquid fertilizer, but the problems of hydroponics and liquid fertilizer cultivation are weak against pathogenic bacteria such as root rot That is. In order to stably grow useful microorganisms and useful lactic acid bacteria in the hydroponic cultivation tank and to optimally manage the environment of the hydroponic cultivation tank, the lactic acid bacteria are stably grown in the lactic acid bacteria culture device equipped with a microbubble generator. It is to replenish.

  Until now, hydroponics tanks and aquaculture tanks are separated separately, and there is a difference in height with hydroponics tanks, and if you make it the same as the aquaculture tank, there is a space in the lower part, using this as a breeding space aquaculture space U-block block tank or concave block tank of the same material on both sides of two rows of rectangular aquarium tanks because the feeding part of the breeding fish is blocked because the upper part is covered with hydroponics part (No drawing) was connected and the upper space was used as a feeding place. The fish could move freely and the breeding place expanded and the feeding place was secured.

  Along with this, the introduction of a block water tank has made it easy to reduce the introduction cost and the subsequent temperature control, and it is easy to transport by making it into a block. ) And amine-group polyether chemical products produced by ARMA urea-bonded finishes, the initial investment and introduction cost issues have progressed towards solving.

  Since this system has a space in the central part, a filtration water tank part was installed in this part, and a place for placing a lactic acid bacteria culture device next to it was secured, and it was possible to further reduce the space. In order to secure the optimal environment for the growth of cultured fish, the occurrence of bacterial infections is controlled by inhibiting the growth of pathogenic bacteria using lactic acid bacteria and inhibiting the occurrence of diseases and enhancing the immune power of cultured fish. These environments are secured in hydroponics tanks, and plant disease prevention measures and ensuring the optimal environment in each tank are the most important issues of this system. Mass growth technology for these lactic acid bacteria and rapid nitrification of ammonia and nitrous acid were achieved with the aid of microbubbles.

  Until now, the problem in realizing complete land culture without complete water exchange has been the removal of nitrate nitrogen, but by adopting plant cultivation, nitrate nitrogen is absorbed and a complete circulation system can be achieved. As a result, it became possible to simultaneously perform aquaculture and plant cultivation in the upper and lower parts of the same tank.

  And the system which can supply useful lactic acid bacteria stably in a water tank was built, and the circulation environment of water was established. The problem was that fish farming profits deteriorated due to fish disease. The solution to this problem is that large quantities of fine particles can be generated to cultivate lactic acid bacteria explosively. In addition, the fine bubbles of microbubbles are useful for the cultivation and proliferation of bacteria and lactic acid bacteria, and the synergistic effect is expected to escape the crisis of bacterial infection and enable stable management.

  In the fish breeding and aquaculture industries, the water exchange system was susceptible to natural disasters because it was bred with a weir in the river and a fish farm in the sea. The effects of disasters can be avoided because aquaculture is possible. In addition, the business of exchanging water was costly due to the cost of electricity and water charges, but if these were lost, it would lead to cost reduction. In addition, since it is an indoor work process, it is possible to participate even if there is no experience or experience in agriculture or fishery.

  And in order to use pupae in the process of producing lactic acid bacteria-based probiotics feed, I was plagued by the peculiar odor that came out of pupae. Lactic acid bacteria solved this. Plant lactic acid bacteria are more effective than animal lactic acid bacteria, and the strong odor of pupae is more effective for fishing that attracts fish. End up. Plant lactic acid bacteria solved this problem.

  In addition, countermeasures against pathogenic bacteria in hydroponics tanks are also a stable supply of useful lactic acid bacteria. Utilizing this odor-improving effect and pathogen-controlling effect, countermeasures and solutions for malodors in food factories, poultry farms, pig farms, etc. In addition, the technology for mass cultivation of lactic acid bacteria using microbubbles, which can be used to combat bad odors of garbage and composting of garbage, expands the industrial applicability.

Overall view of a water tank with a moving partition with a net-type biofiltration device with a built-in micro / nanobubble generator and a box-type biofiltration device Plan view of water tank with each device and partition plate of the present invention The filtration part with a micro / nano bubble generator of the present invention, a net type biofiltration device and a box type biofiltration device overall view and cross-sectional view Overall view of the combined biofiltration device of the filtration device of the present invention, a box-type biofiltration device with a micro / nanobubble generator and a net-type biofiltration device Inside view of the filtration device for aquarium of the present invention, a combined biofiltration device with a microbubble generator Micronutrient supplementation system in hydroponics tank of the present invention Overall view and cross-sectional view of the net-type biofiltration device of the present invention attached with a submersible pump type micro / nano bubble generator The figure which attached the submersible pump type micro nano bubble generator to the box type biofiltration device of the present invention, and a sectional view Overall view and sectional view of lactic acid bacteria culture apparatus of the present invention The front view of the whole system which connected culture and hydroponics using the water tank filtration device of the present invention, and the lactic acid bacteria culture device General view of a system in which an aquaculture tank and a hydroponic culture tank and a lactic acid bacteria culture apparatus using the filtration apparatus for aquarium of the present invention are connected Sectional view of the whole system in which aquaculture and hydroponic cultivation using the aquarium filtration device of the present invention and a lactic acid bacteria culture device are connected, and a partition plate in the lactic acid bacteria culture device The hydrogel floor, the micro / nano bubble generator installation sectional view, and the sectional view which connected the lactic acid bacteria culture device with a submersible pump type micro nano bubble generator to the hydroponics water tank of the present invention. (Viewed from the K ← K ← side. Enlarged sectional view of the portion of K ← K ← in FIG. 11) A plan view in which a U-shaped corner water tank is attached to a rectangular two-row water tank blocked according to the present invention, and a filtration water tank part and a lactic acid bacteria culture apparatus part are arranged inside. The front view which installed the U-shaped corner part water tank blocked in the rectangular-shaped two-line water tank blocked according to the present invention, and arranged the filtration water tank part and the lactic acid bacteria culture device part inside Cross-sectional view of a rectangular corner water tank made into a block according to the present invention and a cross-sectional view (right side) where a lactic acid bacteria culturing device section as viewed from B ← to B ← side in FIG. The top view and sectional view of the U-shaped corner section water tank which made the present invention block A plan view and a cross-sectional view of the rectangular water tank of the present invention Example 1 Photograph of nitrifying ammonia to nitrous acid Example 1 Experimental data for nitrifying ammonia to nitrous acid Example 2 Lactic Acid Bacteria Culture and Culture Data Utilizing Symbiotic Relationship between Lactobacillus and Natto Tomato hydroponics photo of Example 3

Embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a general view of a water tank in which the filtration apparatus 1 and 2 are accommodated and the filtration apparatus hidden partition plate 5 can move and is fixed to 6 fixed to the water tank by two joints 7 fixed to the partition plate. is there. FIG. 2 is a plan view of the water tank 3 with the moving partition plate 5.
FIG. 3 is an overall view of two types of water tank filtration units with moving partition plates 5. Conventionally, the partition plate is fixed to the water tank and the filter medium is directly taken in and out. In addition, since there is a flange 71 for preventing water pressure, it is possible to avoid putting in and out the filter medium and the net-type biofiltration device in a fixed space, and to make the net-type biodevice 1 and the box-type biodevice 2 independent. I devised a new way to move 5 and close the front side.

  FIG. 4 shows the overall view of the device 21 in which the net-type biofiltration device 1 and the box-type biofiltration device 2 are combined, and the lid 25 removed. FIG. 5 shows a general view drawn with the outside removed, a sectional view of an air pump, a microbubble generator 13 used by being inserted into a bottom filtration punch plate 15 and a normal air diffuser air stone 16.

  Fig. 6 shows a micronutrient replenishment system that provides important nutrients to plants in addition to nitrate nitrogen. A trace element supply tank 32 is installed in a hydroponics floor tank 36, and a trace element solution is supplied from there. Refill from tank 32. There is a permeation prevention receptacle 48 and a cap liquid stopper 26 by disposing a root-penetrating permeation tube or hydrofilm utilization tube 41 on the surface of the hydrogel film, and an overall view and a cross-sectional view of supplying trace elements and deficient nutrients to plants. is there.

  FIG. 7 shows the submersible pump 44 and the microbubble generator 51 connected to the net-type biofiltration device 1 and attached and fixed to the bottom net box portion 17, and the air stone 16 and the submersible pump attached to the top of the bottom net box portion 17. It is a general view and a sectional view of a micro-bubble generating biofiltration device 62.

  FIG. 8 shows an underwater device in which a submersible pump and a microbubble generator 51 are connected to a box-type biofiltration device below the bottom filtration punch plate 15 and a conventional scattering tube air stone 16 is attached to the punch plate. It is the whole figure and sectional view of pump type microbubble generation box biofiltration device 63.

  FIG. 9 shows an overall view of the lactic acid bacteria culture device 53 and the generator 51 fed with water from the adjacent hydroponics aquarium with the submersible pump 44, replenishes the air with the self-feeding pipe 57, and raises the bubbles from the microbubble generator 51. It discharges | emits toward the inoculum net bag 50 which rides on the net shelf 49, and, thereby, lactic acid bacteria are cultured.

  FIG. 10 shows the culture water sent from the aquaculture tank 43 to the filtration water tank 46, and the filtered culture water absorbed in the hydroponic cultivation water tank 36 was sent to the lactic acid bacteria culture device 53, and mixed with the lactic acid bacteria. FIG. 3 is a front view of the circulation system that returns to the aquaculture tank 43.

  11 shows the net-type biofiltration device 62 of FIG. 3 in which the culture sewage containing ammonia generated in the aquaculture tank 43 is stored in the filtration water tank, the box-type biofiltration device 63, the combined biofiltration device 21 of FIG. Furthermore, ammonia and nitrous acid are nitrified by the net type 62 to which the submersible pump of FIG. 7 and FIG. 8 and the microbubble generator are connected and the box type biofiltration device 63 to leave nitrate nitrogen, and the next hydroponics It is sent to the cultivating aquarium 36 where it is absorbed from the roots as a nutrient source for the plant, and there are no substances harmful to aquatic organisms or plants in the water. Therefore, the circulation system that cultivates lactic acid bacteria called macrophages (macrophages) that have the ability to suppress the growth of dangerous harmful bacteria when breeding in the aquatic environment and sends them to aquaculture and hydroponics tanks. FIG.

  FIG. 12 is a cross-sectional view connected to the hydroponics tank 36, the lactic acid bacteria culture device 53, and the aquaculture tank 43 and a diagram of the partition plate 59.

  FIG. 13 is an enlarged cross-sectional view of a portion from K ← to K ← in FIG. There is a microbubble generator 51 installed on the bottom of the hydroponics tank 36, a net panel 47 for placing a hydrogel film on the top, and a hydrogel film 34 for planting a plant. Water is supplied to the culture device 53. The generator 51 that has taken in water from the fed water and the self-contained pipe 57 discharges microbubbles and hangs on the inoculum: bag 50 placed on the upper net shelf 49. Lactic acid bacteria whose cells are activated by the fine bubbles of microbubbles grow in the culture apparatus.

  Up to now, hydroponics has been either placing the cultivation tank on the ground or preparing a stand in consideration of work efficiency and placing it on it, either of which is inefficient at the top or bottom. 14 is an overall view of a circulation system connected to two rows of block aquariums 66 by a circulation type U-shaped or concave (not shown) block aquarium 65. . Two rows of aquariums connected by U-shaped and concave block aquariums 65 have a space in the center, and the filtered water tank 46 is filled with the sewage from the aquaculture sewage inflow port 38 from the net-type biofiltration device 1 to the partition plate 70. Filter through the box-type filtration device 2, the combined bio-filtration device 21, the net-type bio filtration device 62 with the submersible pump microbubble generator 51, and the box-type biofiltration device 63 with the submersible pump microbubble generator. The mixed water enters from the filtered water intake 61 (not shown) at the bottom of the lactic acid bacteria culture apparatus 53 (mixed with the lactic acid bacteria cultivated) (from the overflow pipe 47) is mixed with filtered water from the outlet 56 and into the water tank from the outlet 69. Return.

  FIG. 15 is a front view of the circulation block water tank 72, where the myobubble generator 13 is provided at the bottom, and a hydrogel film 34 and a hydroponics floor receiving net panel 27 are prepared at the top.

  FIG. 16 is a cross-sectional view of the circulating water tank 72. A hydroponics floor device 27 (using a net at the bottom) 27 is prepared on a crosspiece 67 on which a hydroponics floor receiving net panel is placed, and a hydrogel film 34 is set thereon. ing. 14 is a cross-sectional view from B ← to B ← in FIG. 14, the microbubble generator 13 is prepared at the bottom of the lactic acid bacteria culture device 53, and there is a net shelf at the top thereof. A system that discharges microbubbles from below (Lactic acid bacteria culture data is collected by inspection with this device, attached)

  FIG. 17 is a plan view and a cross-sectional view of a corner block water tank 65 of U shape and concave shape (not shown).

FIG. 18 is a plan view and a sectional view of the rectangular block water tank 66.
The aquarium for carrying out these aquaculture and plant cultivation was made of expensive concrete, FRP, or cheap cloth. These products are unstable in maintaining and managing the water temperature, and when they are large, construction and transportation on site are difficult and expensive.

  In order to solve this problem, each part is made into a block and can be easily bonded with a water tank that is assembled on site, and the larger size can be planned and industrialized if the water pressure is taken into account. For example, on-site construction is easy with a spray system, light on-site construction is easy during transportation, surface treatment and water leakage countermeasures are generated by a chemical reaction of isocyanate (curing agent) and amine group polyether, reaction of isocyanate and amine Is faster than the reaction between isocyanate and water, so that a strong ARMA urea bond is formed in a chemically stable and short time. And a firm product is completed as an aquarium. This reaction cures in seconds to seconds to tens of seconds.

Example 1: Comparison experiment between micro bubbles and air stone (aeration tube) when ammonia is nitrified to nitrite by nitrifying bacteria.
Two water tanks (with 90 liters) are prepared this time, and an air stone is attached to the bottom of the net biofilter and a microbubble generating device is attached to the N side.

At 7:00 on August 7, 2010, 5 cc is put into each water tank in the left S water tank and the right N water tank (Wako Pure Chemical Industries, Ltd. ammonia nitrogen standard solution) (Photo 1). Photo 2 shows S and N water tanks with values of ammonia concentration of 5.0 mg / L or more. Since the number of bacteria in the net bio device is small, the value does not change until August 20, and the ammonia in the S tank and N tank reaches 0 mg / L at 21:00 on August 21 (Photo 3).
At 21:30 on August 21st, 5cc of ammonia is put into the S and N tanks (Photo 4). At 7:00 on August 23, S and N tanks became 0 mg / L (Photo 6). It was found that the numerical values of the S and N water tanks did not change, and the fine bubbles of the microbubbles on the N water tank side could not completely enter the filter medium.

  Therefore, along with the microbubble generator attached to the bottom of the net biofilter on the N water tank side, the conventional air stone (aeration tube) is used in combination and returned to the N water tank. At 7:30 on August 23, 5 cc of ammonia solution was added to the N and S water tanks and inspected with reagents, confirming that it was 5.0 mg / L or more (Photos 7 and 8). Tested at 7:30 on August 24th, 0 mg / L only on the N water tank side, S water tank becomes 0.25-1.5 mg / L, and here for the first time S water tank (air stone) and N water tank (micro bubble The difference in the numerical value of the generator and air stone) was confirmed, and it was confirmed that the N water tank was nitrified with ammonia in 24 hours (Photo 9). 5cc of the solution is added and it is confirmed that the value becomes 5.0 mg / L or more at 10:30 (Photos 10 and 11). At 10:00 on August 26, it was confirmed that the test was 0 mg / L, and in 24 hours, ammonia was nitrified and changed to nitrous acid (Photo 12).

  This combined system of microbubble generator and air stone (aeration tube) has the ability of micro / nano bubbles that generate fine bubbles, so it does not get into the filter material and has few opportunities to contact nitrifying bacteria. There wasn't. Nitrifying bacteria are absolute aerobic bacteria, and the presence of oxygen is essential for their growth. Therefore, shortening the nitrification time requires supplementation of oxygen to the nitrifying bacteria. Therefore, a microbubble generator and air stone are used in combination. As a result, oxygen can be replenished to every corner of the biofiltration device. Therefore, it took 35 hours from 22:30 on August 21 to 7:00 on August 23 in the second inspection after the filtration system was started up. After that, the combination of micro / nano bubble generator and air stone 2 Each inspection is within 24 hours. Submit photo (Figure 19-1) and experimental data (Figure 19-2).

Example 2: Results of the number of lactic acid bacteria and ph value
This time, two micro / nano bubble generators are installed separately at the bottom of two net containers, and 15 g of Shiso leaf salted and 15 g of apple boiled in sugar are prepared in two net bags. Is closed and put into a net container, and the remaining net bag is filled with 10 g of dry natto, which is a saccharified natto bacterium, alive. Two tanks are prepared for 90 liters, and the tank containing the net bag containing the shiso leaves, dried natto, and apple sugar boiled is called the sn side. The water tank containing the net bag is called the n-side water tank. Shiso leaves are abundant with plant lactic acid bacteria, preserved by salting them, and boiled apples are secured as food for lactic acid bacteria. It is said that the lactic acid bacteria grow about 100 times after culturing due to the symbiotic relationship between lactic acid bacteria and saccharifying natto bacteria.

  In this experiment, there are numerical data of the growth of a culture solution of sn lactic acid bacteria containing dried natto and a single culture medium of n lactic acid bacteria. Submit experimental data photo (Figure 20).

Example 3: Seawater culture and hydroponics Hydrogel films are suitable for plant cultivation because they pass nutrients such as ions, amino acids, and sugars. Seawater aquaculture and general plant cultivation are not possible due to the problem of salt damage, but for example, the African baraf plants are resistant to salt and contain salt in the body, so seawater aquaculture and seawater fish farming Is possible. In addition, it is possible to grow tomatoes on this film, and the newly developed biofiltration device is more capable of filtering seawater than fresh water. For this reason, it is possible to select a plant that is resistant to salt and achieve seawater aquaculture and seawater hydroponics at the same time.
In the experiment, we succeeded in cultivating the tomatoes from the seeds by filtering the feces and uneaten bait (remove ammonia and nitrous acid with a filtration system). This method mixes a little seawater with the feces and uneaten bait. Was added daily to a freshwater aquarium to give a filtered version. Submit a photo of the experiment (Figure 21).

DESCRIPTION OF SYMBOLS 1 Net-type biofiltration apparatus 2 Box-type biofiltration apparatus 3 Water tank with filtration apparatus concealed moving partition plate 4 Lower water intake port 5 Movement partition plate 6 Fixed partition plate 7 Portion where movable partition plate is joined to fixed partition 8 Air or Air pump (through tube or pipe) leading to water discharge pipe 9 punch plate 10
11 Plaster for air discharge,
12 Air or water discharge pipe 13 Micro / nano bubble generator (compressor)
14 Packing 15 Punch plate for bottom filtration 16 Air stone (aeration tube)
17 Net box 18 for bottom surface Filter material (co-ga stone, foam glass)
19 Expansion prevention net box 20 Stone injection hole 21 for generating air stone and micro / nano bubble bubble 21 Combined biofiltration device. (To reach the air compressor)
22 In the combined biofiltration device, the partition plate 23 Bottom water intake 24 Upper water transfer port 25 Combined filtration device upper lid (with air or water discharge pipe through hole)
26 Cap stopper 27 Hydroponics floor equipment (uses net at bottom)
29 Water tank window 30 Drip needle or insertion tube 31 Hydrogel material using protrusion 32 Trace element supply tank 34 Hydrogel film 35 Filtration hidden partition stopper 36 Hydroponic water tank 37 Trace element supply toyo 38 Cultured sewage Inlet 39 Cultured sewage outlet 40 Trace element supply rod 41 Trace element supply tube 43 Aquaculture tank 44 Submersible pump 45 Water supply pipe 46 Water tank for filtration device 47 Hydrogel film receiving net panel 48 Penetration prevention receptacle 49 Net shelf (of lactic acid bacteria Put the inoculum)
50 Inoculum bag of lactic acid bacteria.
51 Submersible Pump Micro / Nano Bubble Generator 52 Air Self-Supply Port 53 Lactic Acid Bacteria Culture Device 55 Pump Connection Pipe 56 Lactic Acid Bacteria Micro / Nano Bubble Water Outlet 57 Air Self-Supply Port Connection Pipe 58 Trace Element Supply Pipe 59 Lactic Acid Bacteria Culture Device Partition Plate 60 Pump Water Intake Mouth 61 Filtration water inlet 62 Net type bio device with submersible pump type micro / nano bubble generator 63 Box type bio unit with submersible pump type micro / nano bubble generator 64 Lid 65 U type block water tank 66 Rectangular block water tank 67 Net panel (cultivation equipment) ) A crosspiece 68 A bottom groove 69 A mixed water outlet 70 A partition plate 71 A flange for water tank 72 A circulating block water tank

Claims (8)

  A micro / nano bubble generating device, a diffuser tube, and a porous material inhabiting nitrifying bacteria are inserted inside the aquarium, and the bubbles generated from the diffuser tube together with the micro / nano bubbles generated by the micro / nano bubble generating device are An aquatic animal culture tank, wherein the micro / nano bubble generator and the air diffuser are arranged so as to be in contact with a porous material.   The micro / nano bubble generator is arranged inside a net-type biofiltration device in which a porous material inhabiting the nitrifying bacteria is inserted into a net and a device in which the air diffuser is arranged at the bottom of the net is integrated. The aquatic aquaculture tank according to claim 1, characterized in that   The micro-nano bubble generator is disposed so that the lactic acid bacteria seed bag containing the lactic acid bacteria directly inserted or the lactic acid bacteria inserted is inserted, and the micro-nano bubbles are in contact with the lactic acid bacteria or the lactic acid bacteria seed bacteria bag. The aquatic aquaculture tank described.   The aquatic aquaculture tank according to claim 3, wherein saccharifying bacteria are mixed together with the lactic acid bacteria.   The aquaculture tank according to claim 1, wherein a hydrogel film is laid in contact with the water surface of the aquaculture tank, and a plant for cultivation is planted on the top surface thereof.   The aquaculture tank according to claim 5, wherein trace elements or nutrients are supplied from an upper surface of the hydrogel film.   The aquaculture tank according to claim 6, wherein the trace element or nutrient is supplied using a trace element supply tube that allows water or an aqueous solution to pass therethrough and does not pass through plant roots.   In the aquatic animal culture tank formed in a ring shape, a purification system in which a micro / nano bubble generating device, a diffuser tube and a porous material inhabiting nitrifying bacteria are inserted is arranged inside the ring of the water tank, Item 8. A tank for aquatic animal cultivation according to item 1.

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