JP2011508185A5 - - Google Patents
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- JP2011508185A5 JP2011508185A5 JP2010534405A JP2010534405A JP2011508185A5 JP 2011508185 A5 JP2011508185 A5 JP 2011508185A5 JP 2010534405 A JP2010534405 A JP 2010534405A JP 2010534405 A JP2010534405 A JP 2010534405A JP 2011508185 A5 JP2011508185 A5 JP 2011508185A5
- Authority
- JP
- Japan
- Prior art keywords
- sample
- ultra
- sample carrier
- rapid freezing
- cooling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims description 204
- 238000007710 freezing Methods 0.000 claims description 136
- 230000008014 freezing Effects 0.000 claims description 135
- 238000001816 cooling Methods 0.000 claims description 86
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- 238000000034 method Methods 0.000 claims description 58
- 238000010438 heat treatment Methods 0.000 claims description 51
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- 239000012472 biological sample Substances 0.000 claims description 46
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 33
- 239000007788 liquid Substances 0.000 claims description 25
- 238000003384 imaging method Methods 0.000 claims description 21
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- 229910052782 aluminium Inorganic materials 0.000 description 2
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- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
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- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US98916507P | 2007-11-20 | 2007-11-20 | |
| US60/989,165 | 2007-11-20 | ||
| PCT/EP2008/009825 WO2009065585A2 (en) | 2007-11-20 | 2008-11-20 | Ultra-rapid freezing device and method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011508185A JP2011508185A (ja) | 2011-03-10 |
| JP2011508185A5 true JP2011508185A5 (https=) | 2012-01-12 |
| JP5261496B2 JP5261496B2 (ja) | 2013-08-14 |
Family
ID=40637943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010534405A Expired - Fee Related JP5261496B2 (ja) | 2007-11-20 | 2008-11-20 | 超急速冷凍装置及び超急速冷凍方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US8256232B2 (https=) |
| EP (1) | EP2224800B1 (https=) |
| JP (1) | JP5261496B2 (https=) |
| WO (1) | WO2009065585A2 (https=) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5261496B2 (ja) | 2007-11-20 | 2013-08-14 | マックス プランク ゲゼルシャフト ツゥアー フェデルゥン デル ヴィッセンシャフテン エー フォー | 超急速冷凍装置及び超急速冷凍方法 |
| US9700038B2 (en) | 2009-02-25 | 2017-07-11 | Genea Limited | Cryopreservation of biological cells and tissues |
| CN103179852B (zh) | 2010-05-28 | 2015-04-08 | 格尼亚有限公司 | 改良的显微操作和储存装置以及方法 |
| WO2012038484A1 (en) * | 2010-09-21 | 2012-03-29 | University Of Utrecht Holding B.V. | Method of preparing a biological sample for inspection with electron microscopy and fluorescent light microscopy |
| US9863670B2 (en) | 2011-09-20 | 2018-01-09 | Lockheed Martin Corporation | Extended travel flexure bearing and micro check valve |
| AT512287B1 (de) * | 2012-03-02 | 2013-07-15 | Leica Microsysteme Gmbh | Vorrichtung zur Lichtstimulation und Kryokonservierung biologischer Proben |
| WO2013152239A1 (en) * | 2012-04-04 | 2013-10-10 | National University Of Singapore | Cryo-preparation systems and methods for near-instantaneous vitrification of biological samples |
| US9784505B2 (en) | 2012-05-15 | 2017-10-10 | Lockheed Martin Corporation | System, apparatus, and method for micro-capillary heat exchanger |
| DE102012014791A1 (de) | 2012-07-25 | 2014-01-30 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren und Vorrichtung zur Hochgeschwindigkeits-Temperierung einer Probe |
| EP2738539A1 (en) * | 2012-11-28 | 2014-06-04 | Fei Company | Signal based sample preparation |
| WO2014085801A1 (en) * | 2012-11-30 | 2014-06-05 | The Broad Institute, Inc. | Cryo-treatment in a microfluidic device |
| CN104378979B (zh) * | 2013-06-21 | 2016-05-11 | 徐小杨 | 活体细胞快速玻璃化冷冻的自动操作方法及系统 |
| US9683766B1 (en) | 2013-07-12 | 2017-06-20 | Lockheed Martin Corporation | System and method for electronic de-clogging of microcoolers |
| JP6173879B2 (ja) * | 2013-10-28 | 2017-08-02 | 日本電子株式会社 | 荷電粒子線装置、および試料容器 |
| US9999885B1 (en) * | 2014-05-30 | 2018-06-19 | Lockheed Martin Corporation | Integrated functional and fluidic circuits in Joule-Thompson microcoolers |
| DE102015100278B3 (de) * | 2015-01-09 | 2016-05-04 | Rheotec Messtechnik Gmbh | Temperieranordnung mit Kombinationswärmeübertrager zur Heizung und Kühlung einer Messplatte eines Messgerätes |
| ES3029563T3 (en) | 2015-12-07 | 2025-06-24 | Coopersurgical Inc | Low temperature specimen carriers |
| EP3179229B1 (en) * | 2015-12-11 | 2019-01-30 | FEI Company | Preparation of cryogenic sample for charged-particle microscopy |
| KR101750745B1 (ko) * | 2016-01-22 | 2017-06-27 | 한국과학기술연구원 | 투과형 엑스선 회절 분석장치용 퍼니스 및 이를 이용한 투과형 엑스선 회절 분석장치 |
| EP3418710B1 (en) | 2016-02-19 | 2022-08-24 | Nikon Corporation | Sample preparation method and sample preparation device |
| EP3260839B1 (en) * | 2016-06-22 | 2021-01-27 | Universiteit Maastricht | Method for preparing samples for imaging or diffraction experiments under cryogenic conditions |
| GB201613173D0 (en) * | 2016-07-29 | 2016-09-14 | Medical Res Council | Electron microscopy |
| CN108168177A (zh) * | 2018-01-29 | 2018-06-15 | 南通天工深冷新材料强化有限公司 | 一种新型生物样品保藏柜 |
| CN110596113B (zh) * | 2019-10-12 | 2021-08-20 | 燕山大学 | 电渗混合效率测量装置 |
| US11856947B2 (en) | 2020-02-17 | 2024-01-02 | Cook Medical Technologies Llc | System for automated permeation of a biological material and method of using same |
| CN111624180B (zh) * | 2020-05-22 | 2021-06-22 | 西安交通大学 | 捕获微通道内运动大分子形态的冷冻荧光显微成像系统及方法 |
| CN114322406B (zh) * | 2020-09-27 | 2023-11-14 | 青岛海尔电冰箱有限公司 | 冰箱 |
| CN112747995A (zh) * | 2020-12-28 | 2021-05-04 | 生物岛实验室 | 冷冻芯片、冷冻系统、样品测试系统及方法 |
| CN115494054B (zh) * | 2021-06-18 | 2025-04-18 | 上海交通大学 | 细胞低温超快速冷冻过程观测的可视化冷冻系统 |
| CN115615037B (zh) * | 2021-07-15 | 2025-10-21 | 中国科学院长春光学精密机械与物理研究所 | 一种微流控芯片制冷系统及控温方法 |
| US20240060864A1 (en) * | 2022-08-19 | 2024-02-22 | Alliance For Sustainable Energy, Llc | In situ biasing and freezing of cyrogenic electron microscopy samples |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4388814A (en) * | 1982-03-26 | 1983-06-21 | Schilling Dean W | Cryogenic device and method |
| US4510169A (en) * | 1983-08-23 | 1985-04-09 | The Board Of Regents, The University Of Texas | Method and apparatus for cryopreparing biological tissue for ultrastructural analysis |
| US4745771A (en) * | 1983-08-23 | 1988-05-24 | Board Of Regents, The University Of Texas System | Apparatus and method for cryopreparing biological tissue for ultrastructural analysis |
| JPS6372601A (ja) * | 1986-09-16 | 1988-04-02 | Hoxan Corp | 生体細胞の凍結装置 |
| US4707998A (en) * | 1986-12-03 | 1987-11-24 | The Board Of Regents, The University Of Texas | Apparatus and method for ultrarapid cooling of biological samples |
| US5044165A (en) * | 1986-12-03 | 1991-09-03 | Board Of Regents, The University Of Texas | Cryo-slammer |
| JPH0814337B2 (ja) * | 1988-11-11 | 1996-02-14 | 株式会社日立製作所 | 流体自体の相変化を利用した流路の開閉制御弁及び開閉制御方法 |
| JP3028826B2 (ja) * | 1990-03-09 | 2000-04-04 | 株式会社日立製作所 | 流体流路の開閉装置及びその開閉制御方法及び閉止確認方法 |
| JPH06167431A (ja) * | 1992-11-27 | 1994-06-14 | Hitachi Ltd | 凍結保存装置 |
| JP3356636B2 (ja) * | 1996-11-08 | 2002-12-16 | 株式会社日立製作所 | 急速凍結装置 |
| DE59805560D1 (de) * | 1997-01-13 | 2002-10-24 | Daniel Studer | Probenhalter für wasserhaltige Proben sowie Verfahren zu deren Verwendung |
| JP3334608B2 (ja) * | 1997-09-17 | 2002-10-15 | 株式会社島津製作所 | 冷却装置および試料導入装置 |
| GB2330516A (en) * | 1997-10-22 | 1999-04-28 | Elizabeth Acton | Cryopreservation of cell suspensions |
| US6403376B1 (en) * | 1998-11-16 | 2002-06-11 | General Hospital Corporation | Ultra rapid freezing for cell cryopreservation |
| US6300130B1 (en) * | 1998-11-16 | 2001-10-09 | The General Hospital Corporation And University Of Massachusetts | Ultra rapid freezing for cell cryopreservation |
| AU2003272397A1 (en) * | 2002-09-16 | 2004-04-30 | Waters Investments Limited | Freeze-thaw valve limiting cryogenic usage |
| JP2004156871A (ja) * | 2002-11-07 | 2004-06-03 | Sumitomo Electric Ind Ltd | 薄型流路形成体その製造方法 |
| DE102004047965B4 (de) * | 2004-10-01 | 2007-03-01 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Kryoeinrichtung und zugehöriges Betriebsverfahren |
| JP5261496B2 (ja) | 2007-11-20 | 2013-08-14 | マックス プランク ゲゼルシャフト ツゥアー フェデルゥン デル ヴィッセンシャフテン エー フォー | 超急速冷凍装置及び超急速冷凍方法 |
-
2008
- 2008-11-20 JP JP2010534405A patent/JP5261496B2/ja not_active Expired - Fee Related
- 2008-11-20 WO PCT/EP2008/009825 patent/WO2009065585A2/en not_active Ceased
- 2008-11-20 EP EP08851857A patent/EP2224800B1/en not_active Not-in-force
- 2008-11-20 US US12/274,653 patent/US8256232B2/en not_active Expired - Fee Related
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