JP2011017721A - Device and method for measuring luminous energy - Google Patents

Device and method for measuring luminous energy Download PDF

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JP2011017721A
JP2011017721A JP2010213947A JP2010213947A JP2011017721A JP 2011017721 A JP2011017721 A JP 2011017721A JP 2010213947 A JP2010213947 A JP 2010213947A JP 2010213947 A JP2010213947 A JP 2010213947A JP 2011017721 A JP2011017721 A JP 2011017721A
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light
amount
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calibration
luminous energy
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Muneki Ran
Takeo Tanaami
健雄 田名網
宗樹 蘭
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Yokogawa Electric Corp
横河電機株式会社
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Abstract

PROBLEM TO BE SOLVED: To provide a device and a method for measuring luminous energy capable of quantitatively evaluating luminous energy.SOLUTION: The device for measuring luminous energy which measures the luminous energy of light captured with a light-detecting means includes a luminous energy calculating means which calculates the luminous energy of fluorescence signal light based on the output signal of the light-detecting means; an excitation light irradiation means of irradiating excitation light; a means of generating standard fluorescence signal which generates a fluorescence signal used as the standard by irradiation of the excitation light; and a calibration means which calibrates the luminous energy calculated by the luminous energy calculating means, by providing the light-detecting means with light having a known luminous energy with the standard fluorescence signal.

Description

本発明は、光量を計測する光量計測装置および光量計測方法に関し、とくに光量を定量評価できる光量計測装置および光量計測方法に関する。 The present invention relates to a light amount measuring apparatus and a light amount measuring method for measuring a light amount, particularly relates to a light amount measuring apparatus and a light amount measuring method capable of quantitatively evaluating the amount of light.

DNA等の生体高分子を同定する方法として、マイクロアレイを用いた方法が知られている。 As a method for identifying a biopolymer such as DNA, a method using the microarray is known. 例えばDNAを同定する場合、既知の塩基配列を有するDNAプローブをマイクロアレイの各サイトに固定し、ハイブリダイゼーションによって相補的な塩基配列をもつDNAを各サイトに結合させるものである。 For example, to identify the DNA, the DNA probe having a known base sequence is fixed to each site of a microarray is a DNA having a complementary base sequence by hybridization intended to be bonded to each site. 結合したDNAを蛍光マーカで標識しておくことで、結合量を蛍光の光量として認識できる。 Bound DNA to that previously labeled with a fluorescent marker, may recognize a binding amount as the light amount of the fluorescence.

光量は専用の光量計測装置を用いて計測する。 Light amount measured by using the light amount measuring device only. しかし従来の装置では、絶対光量が計測できないため、遺伝子発現量を直接定量評価できない。 However, the conventional apparatus, since the absolute amount of light can not be measured, can not be directly quantitatively evaluated the gene expression level.

本発明の目的は、光量を定量評価できる光量計測装置および光量計測方法を提供することにある。 An object of the present invention is to provide a light amount measuring apparatus and a light amount measuring method capable of quantitatively evaluating the amount of light.

本発明の光量計測装置は、光検出手段で取り込んだ光の光量を計測する光量計測装置において、前記光検出手段の出力信号に基づいて蛍光信号光の光量を算出する光量算出手段と、励起光を照射する励起光照射手段と、この励起光を照射することにより、基準となる蛍光信号を発生する基準蛍光信号発生手段と、前記基準となる蛍光信号により既知の光量の光を前記光検出手段に与えることで、前記光量算出手段により算出される光量を校正する校正手段と、を備えることを特徴とする。 Light amount measuring apparatus of the present invention, the light amount measuring device for measuring the amount of light captured by the light detecting means, a light amount calculating means for calculating a light amount of fluorescent signal light based on an output signal of said light detecting means, the excitation light excitation light irradiating means for irradiating, by irradiating the excitation light, and the reference fluorescent signal generating means for generating a fluorescent signal as a reference, said light detecting means light of a known light intensity by the fluorescent signal serving as the reference by providing the, characterized in that it comprises a calibration means for calibrating the amount of light calculated by the light amount calculating section.
この光量計測装置によれば、別途校正用光源を具備せずに既知の光量の光を光検出手段に与えることができると共に、光量算出手段により算出される光量を校正するので、光量を定量的に評価できる。 According to the light amount measuring device, it is possible to provide a light detecting means light of a known amount without including the calibration light source separately, so calibrating the light amount calculated by the light amount calculating means, quantitative light quantity It can be evaluated on.

本発明の光量計測方法は、光検出手段で取り込んだ光の光量を計測する光量計測方法において、前記光検出手段の出力信号に基づいて蛍光信号光の光量を算出するステップと、励起光照射手段から基準となる蛍光信号を発生する基準蛍光信号発生手段に励起光を照射するステップと、前記基準となる蛍光信号により既知の光量の光を前記光検出手段に与えることで、前記光量算出手段により算出される光量を校正するステップと、を備えることを特徴とする光量計測方法。 Light amount measuring method of the present invention is a light amount measuring method for measuring the amount of light captured by the light detecting means, calculating a light amount of fluorescent signal light based on an output signal of said light detecting means, the excitation light illumination means a step of irradiating excitation light to a reference fluorescent signal generating means for generating a fluorescent signal as a reference from the fluorescent signal serving as the reference by giving the light of known amount on said light detecting means, by the light amount calculating means light amount measuring method characterized by comprising the steps of: calibrating the light amount calculated, the.
この光量計測方法によれば、別途校正用光源を具備せずに既知の光量の光を光検出手段に与えることができると共に、光量算出手段により算出される光量を校正するので、光量を定量的に評価できる。 According to the light amount measuring method, it is possible to provide a light detecting means light of a known amount without including the calibration light source separately, so calibrating the light amount calculated by the light amount calculating means, quantitative light quantity It can be evaluated on.

本発明の光量計測装置によれば、別途校正用光源を具備せずに既知の光量の光を光検出手段に与えることができると共に、光量算出手段により算出される光量を校正するので、光量を定量的に評価できる。 According to the light amount measuring apparatus of the present invention, it is possible to provide a light detecting means light of a known amount without including the calibration light source separately, so calibrating the light amount calculated by the light amount calculating means, the amount of light It can be quantitatively evaluated.

本発明の光量計測方法によれば、別途校正用光源を具備せずに既知の光量の光を光検出手段に与えることができると共に、光量算出手段により算出される光量を校正するので、光量を定量的に評価できる。 According to the light amount measuring method of the present invention, it is possible to provide a light detecting means light of a known amount without including the calibration light source separately, so calibrating the light amount calculated by the light amount calculating means, the amount of light It can be quantitatively evaluated.

本実施形態の光量計測装置の光学系の構成を示す図。 Diagram showing a structure of an optical system of a light amount measuring apparatus of the present embodiment. 本実施形態の光量計測装置の制御系の構成を示すブロック図。 Block diagram showing a configuration of a control system of the light amount measuring apparatus of the present embodiment.

以下、図1および図2を参照して、本発明による光量計測装置の一実施形態について説明する。 Hereinafter, with reference to FIGS. 1 and 2, it will be described an embodiment of a light amount measuring apparatus according to the present invention.

図1は本実施形態の光量計測装置の光学系の構成を示す図である。 Figure 1 is a diagram showing a configuration of an optical system of a light amount measuring apparatus of the present embodiment. 本実施形態は、DNAマイクロアレイ(マイクロアレイ上のDNAやRNAサンプル(以下バイオチップという。))に励起光を照射し、DNAマイクロアレイで発生する蛍光信号光の光量を計測する光量計測装置である。 This embodiment, DNA microarrays (on the microarray DNA or RNA sample (hereinafter referred to as biochip.)) Was irradiated with exciting light, a light amount measuring apparatus for measuring the amount of fluorescent signal light generated by DNA microarrays.

図1に示すように、本実施形態の光量計測装置は、DNAマイクロアレイ(バイオチップ)30に励起光を照射するための光学系として、励起光を発生する緑色レーザ光源1および赤色レーザ光源2と、緑色レーザ光源1からの照射光を折り曲げるミラー3と、それぞれ、緑色レーザ光源1および赤色レーザ光源2からの照射光の光路に配置されるダイクロイックミラー5、マイクロレンズアレイ6、ミラー7、ダイクロイックミラー8および可動ミラー9と、を備える。 As shown in FIG. 1, a light amount measuring apparatus of the present embodiment, as an optical system for irradiating excitation light to a DNA microarray (biochip) 30, a green laser light source 1 and the red laser light source 2 for generating excitation light , a mirror 3 for bending irradiated light from the green laser light source 1, respectively, a dichroic mirror 5 which is disposed in an optical path of the illumination light from the green laser light source 1 and the red laser light source 2, the microlens array 6, the mirror 7, the dichroic mirror comprising 8 and a movable mirror 9, a.

緑色レーザ光源1および赤色レーザ光源2の波長は、cy3やcy5の蛍光色素の励起光に合致するものである。 Wavelength of the green laser light source 1 and the red laser light source 2 is one that matches the excitation light of the fluorescent dye cy3 or cy5.

また、本実施形態の光量計測装置は、DNAマイクロアレイ30で発生する蛍光信号光を受けるための光学系として、信号光の光路に配置されたリレーレンズ11と、リレーレンズ11を通過した光を受ける高感度のCCDカメラ12と、を備える。 Moreover, light amount measuring apparatus of the present embodiment, as an optical system for receiving fluorescent signal light generated at the DNA microarray 30, a relay lens 11 disposed in the optical path of the signal light, receives the light passing through the relay lens 11 includes a CCD camera 12 sensitive, the.

さらに、本実施形態の光量計測装置は、光量の校正を行うための光学系として、校正のためのレーザ光を出力する校正用レーザ光源21と、校正用レーザ光源21から出力されたレーザ光を減衰させる光アッテネータ22と、を備える。 Moreover, light amount measuring apparatus of the present embodiment, as an optical system for calibrating the light amount, the calibration laser light source 21 for outputting laser light for calibration, the laser light outputted from the calibration laser light source 21 It includes an optical attenuator 22 for attenuating the. なお、校正用レーザ光源および光アッテネータ22は、装置に内蔵する代りに校正時に取り付けるようにしてもよい。 Incidentally, the calibration laser light source and the optical attenuator 22 may be attached during calibration instead of the built-in device.

また、CCDカメラ12の前面側には、CCDカメラ12への入射光を遮断する校正用シャッター23が設けられている。 In front of the CCD camera 12, the calibration shutter 23 for blocking the incident light to the CCD camera 12 is provided.

図1に示すように、DNAマイクロアレイ30はテーブル41に載置される。 As shown in FIG. 1, DNA microarray 30 is mounted on the table 41.

図2は本実施形態の光量計測装置の制御系の構成を示すブロック図である。 Figure 2 is a block diagram showing a configuration of a control system of the light amount measuring apparatus of the present embodiment.

図2に示すように、本実施形態の光量計測装置は、CCDカメラ12で捉えた光の光量を算出する光量算出部51と、校正用シャッター23を駆動する校正用シャッター駆動部53と、ミラー9を駆動するミラー駆動部55と、光量算出部51の校正を行う校正部56と、緑色レーザ光源1、赤色レーザ光源2、CCDカメラ12、光量算出部51、校正用レーザ光源21、光アッテネータ22、校正用シャッター駆動部53、ミラー駆動部55および校正部56を制御する制御部61と、を備える。 As shown in FIG. 2, the light amount measuring apparatus of the present embodiment includes a light amount calculating section 51 for calculating the quantity of light captured by the CCD camera 12, a calibration shutter driving section 53 for driving the calibration shutter 23, a mirror a mirror driving section 55 for driving the 9, and calibration unit 56 for calibrating the light amount calculating section 51, the green laser light source 1, the red laser light source 2, CCD camera 12, the light amount calculating section 51, the calibration laser light source 21, an optical attenuator 22, the calibration shutter driving section 53, a control unit 61 for controlling the mirror driving section 55 and the calibration unit 56, a.

次に、DNAマイクロアレイ30の測定時の動作について説明する。 Next, the operation at the time of measurement of the DNA microarray 30.

測定時には、可動ミラー9および校正用シャッター23は、それぞれ光路から退避した破線で示す位置に駆動される(図1)。 During the measurement, the movable mirror 9 and the calibration shutter 23 is driven to the position indicated by the broken line retracted from the optical path respectively (Figure 1).

緑色レーザ光源1または赤色レーザ光源2で出力されたレーザ光はミラー3、ダイクロイックミラー5、マイクロレンズアレイ6、ミラー7、およびダイクロイックミラー8を介してDNAマイクロアレイ30に照射される。 Green laser light source 1 or the red laser laser light output by the light source 2 is a mirror 3, the dichroic mirror 5, the microlens array 6, via the mirror 7 and the dichroic mirror 8 and is irradiated to the DNA microarray 30.

レーザ光による励起により発生したDNAマイクロアレイ30からの蛍光信号光は、ダイクロイックミラー8およびリレーレンズ11を介してCCDカメラ12に入射する。 Fluorescent signal light from the DNA microarray 30 generated by excitation by laser light enters the CCD camera 12 via the dichroic mirror 8 and the relay lens 11. CCDカメラ12の出力信号は光量算出部51に送られ、光量算出部51では信号光の光量が算出される。 The output signal of the CCD camera 12 is sent to the light amount calculating section 51, the light amount of the signal light quantity calculating unit 51 is calculated.

なお、バックグラウンドノイズに埋もれるような低レベルの信号光については、繰り返し加算によるアベレージング処理でランダムなノイズを相対的に低減し、S/N比を向上させてもよい。 Note that the low level of the signal light as buried in background noise, random noise averaging process by repeatedly adding relatively reduced, may improve the S / N ratio.

一般に遺伝子発現量は広いレンジに分布するため、遺伝子発現量を定量的に計測するためには、低いレベルまで光量を高精度に算出することが要求される。 In general for gene expression amount is distributed in a wide range, in order to quantitatively measure the gene expression amount, it is required to calculate the amount of light with high accuracy to a low level. しかし、低レベルの光量は背景光(バックグラウンドノイズ)に埋もれやすく、とくに低レベルの光量において算出精度が問題となる。 However, the amount of low level easily buried in the background light (background noise), in particular calculation accuracy at low levels of light quantity becomes a problem.

次に、校正時の動作について説明する。 Next, a description will be given of the operation at the time of calibration.

本実施形態では、CCDカメラ12の無信号状態を作り、ゼロ点調整を行う。 In this embodiment, creating a no-signal state of the CCD camera 12, performs zero-point adjustment. このとき、校正用シャッター23は光路を遮断する実線で示す位置に駆動される(図1)。 In this case, the calibration shutter 23 is driven to the position indicated by the solid line to block the optical path (Fig. 1). これにより、迷光がCCDカメラ12に入らない状態が確保される。 Thus, a state in which stray light does not enter the CCD camera 12 is ensured. このときのCCDカメラ12の出力信号は光量算出部51に送られ、校正部56は光量算出部51に対し、この出力信号値を基準(ゼロ点)とする校正を実行する。 The output signal of the CCD camera 12 at this time is sent to the light amount calculating section 51, the calibration unit 56 to the light amount calculating section 51, to perform the calibration of the output signal value as a reference (zero point).

また、本実施形態では、CCDカメラ12に入射する光量を制御することで、とくに低光量領域での光量算出の校正を行う。 Further, in the present embodiment, by controlling the amount of light incident on the CCD camera 12, in particular to calibrate the light intensity calculator in low light areas. このとき、校正用シャッター23は光路から退避する破線で示す位置に駆動される。 In this case, the calibration shutter 23 is driven to the position indicated by the broken line retracted from the optical path. また、可動ミラー9は光路に進入した実線で示す位置に駆動される(図1)。 Further, the movable mirror 9 is driven to the position indicated by the solid line that enters the optical path (Fig. 1).

図1に示すように、校正用レーザ光源21から出力された光は光アッテネータ22により減衰され、可動ミラー9およびリレーレンズ11を介してCCDカメラ12に入射する。 As shown in FIG. 1, light outputted from the calibration laser light source 21 is attenuated by the optical attenuator 22, is incident on the CCD camera 12 via the movable mirror 9 and the relay lens 11. 校正用レーザ光源21から出力されるレーザ光の光量、および光アッテネータ22による減衰量は既知であるため、CCDカメラ12に入射される光量も既知である。 Since the laser light quantity to be outputted from the calibration laser light source 21, and the attenuation amount of the optical attenuator 22 is known, the amount of light incident on the CCD camera 12 is also known. また、校正用レーザ光源21から出力されるレーザ光は、例えば、cy3の蛍光信号光の波長(570nm)に近い波長を有する。 The laser beam outputted from the calibration laser light source 21, for example, having a wavelength close to the wavelength (570 nm) of the fluorescent signal light cy3.

光アッテネータ22の減衰量を切り替えることで、例えば、0dBm、−10dBm、−20dBm等、正しく値付けされた光をCCDカメラ12に入射させる。 By switching the attenuation amount of the optical attenuator 22, for example, 0dBm, -10dBm, -20 dBm or the like, is incident correctly priced light to the CCD camera 12. このときのCCDカメラ12の出力信号は光量算出部51に送られ、校正部56は各出力信号値がそれぞれの光量に正しく対応するように、光量算出部51に対する校正を実行する。 The output signal of the CCD camera 12 at this time is sent to the light amount calculating section 51, the calibration unit 56 the output signal value to correspond correctly to the respective light quantity to perform the calibration for the light amount calculating section 51.

このように本実施形態では、校正用シャッター23を用いてゼロ点の校正を行うとともに、光アッテネータ22の減衰量を切り替えることで、とくに低レベルの光量のリニアリティ補正を行うことができる。 As described above, in this embodiment, it performs calibration of the zero point by using the calibration shutter 23, by switching the attenuation amount of the optical attenuator 22 can be particularly performing linearity correction of low levels of light intensity. このため背景光に近い低レベルまで、光量を定量的に正確に計測できる。 Therefore to low level close to the background light, it can be quantitatively measured accurately the amount of light. また、背景光の光量を正しく評価できる。 Also, it can properly evaluate the light intensity of the background light.

上記実施形態では、校正用レーザ光源21と光アッテネータ22とを用いて、既知の光量の光をCCDカメラ12に与えているが、光量を調整可能な光源をこれらに代えて用いてもよい。 In the above embodiment, by using the calibration laser light source 21 and the optical attenuator 22, while giving light of a known light intensity to the CCD camera 12, an adjustable light source light quantity may be used instead of these. 例えば、光源としてLEDを用い、駆動電流によって光量を制御してもよい。 For example, using an LED as a light source, it may be controlled quantity by the drive current.

また、校正用光源として、レーザ光源に代えて白色光源を用いてもよい。 Further, as a calibration light source may be used a white light source instead of a laser light source.
さらに、校正用光源と光アッテネータの組み合わせではなく、基準となる蛍光信号を発生する対象物(基準蛍光信号発生手段)をバイオチップ30の代わりに設置し、そこに緑色レーザ光源や赤色レーザ光源(励起用光照射手段)から励起光を照射し、校正用光源として用いても良い。 Further, instead of the combination of the calibration light source and the optical attenuator, installed object generates fluorescence signal as a reference (reference fluorescent signal generating means) in place of the biochip 30, there green laser light source and a red laser light source ( irradiated with excitation light from the excitation light irradiation means), it may be used as a calibration light source. また、異なる基準蛍光信号を発生する対象物を複数用意することにより、リニアリティ校正ができる。 Further, by preparing a plurality of objects for generating different reference fluorescent signals can linearity calibration.

上記実施形態では、CCDカメラ12に入射する光の光量を正確に制御することで、光量の校正を行っているが、CCDカメラ12に入射する光量を正確に測定してもよい。 In the above embodiment, by precisely controlling the amount of light incident on the CCD camera 12, is performed to calibrate the amount of light, the quantity of light incident on the CCD camera 12 may be accurately measured. 例えば、CCDカメラ12の位置に、光量が正確に測定できるパワーメータを取り付けることで、実際にCCDカメラ12に入射する光量を正確に測定し、同一条件でのCCDカメラ12の出力信号と、予め測定された光量とを対応付けるように校正することができる。 For example, the position of the CCD camera 12, the amount of light by attaching a power meter capable of accurately measuring actually accurately measure the amount of light entering the CCD camera 12, an output signal of the CCD camera 12 under the same conditions, in advance a measured amount of light can be calibrated to associate. このようなパワーメータを用いた校正方法として、特開2004−191232号公報に開示された方法を本発明に適用することができる。 As a calibration method using the power meter, a method disclosed in JP-A-2004-191232 can be applied to the present invention.

上記実施形態では、校正に用いる校正部を装置に設けているが、校正のための校正部を校正時に取り付けることで、同様の校正方法を実行してもよい。 In the above embodiment, it is provided with the calibration unit used to calibrate the apparatus, by attaching the calibrating section for calibration when calibration may be performed similar calibration method. ここで校正部とは、校正用光源21,光アッテネータ22および校正用シャッタ23である。 The calibration unit where the calibration light source 21, an optical attenuator 22 and the calibration shutter 23.

上記実施形態では、DNAマイクロアレイを測定対象とする場合を例示したが、本発明は、タンパク質、糖鎖、メタボローム等、種々の生体物質の検出に対し同様に適用できる。 In the above embodiment, a case has been exemplified that the DNA microarray measured, the present invention is a protein, carbohydrate, metabolome, etc., it can be similarly applied to detection of various biological materials. また、本発明はマイクロアレイによる物質検出に止まらず、半導体プロセスにおける蛍光性塵埃、プラズマディスプレイパネルの蛍光面の評価等にも適用できる。 Further, the present invention does not stop the material detection by microarray can be applied fluorescent dust, in evaluation of the phosphor screen of a plasma display panel in a semiconductor process.

本発明の適用範囲は上記実施形態に限定されることはない。 Scope of the present invention is not limited to the above embodiment. 本発明は、光検出手段の出力信号に基づいて蛍光信号光の光量を算出する光量計測装置および光量計測方法に対し、広く適用することができる。 The present invention is, with respect to the light amount measuring apparatus and a light amount measuring method for calculating a light amount of fluorescent signal light based on an output signal of the light detecting means can be widely applied.

1 緑色レーザ光源(励起光照射手段) 1 green laser light source (excitation light irradiating means)
2 赤色レーザ光源(励起光照射手段) 2 red laser light source (excitation light irradiating means)
12 CCDカメラ(光検出手段) 12 CCD camera (light detecting means)
21 校正用レーザ光源(校正用光源) 21 calibration laser light source (calibration source)
22 光アッテネータ(校正用光源) 22 optical attenuator (calibration source)
23 校正用シャッター 51 光量算出部(光量算出手段) 23 calibration shutter 51 light quantity calculating section (light amount calculating unit)
56 校正部(校正手段) 56 Calibration section (calibration means)

Claims (2)

  1. 光検出手段で取り込んだ光の光量を計測する光量計測装置において、 In the light amount measuring device for measuring the amount of light captured by the light detecting means,
    前記光検出手段の出力信号に基づいて蛍光信号光の光量を算出する光量算出手段と、 A light amount calculating means for calculating a light amount of fluorescent signal light based on an output signal of said light detecting means,
    励起光を照射する励起光照射手段と、 Excitation light irradiating means for irradiating excitation light,
    この励起光を照射することにより、基準となる蛍光信号を発生する基準蛍光信号発生手段と、 By irradiating the excitation light, and the reference fluorescent signal generating means for generating a fluorescent signal as a reference,
    前記基準となる蛍光信号により既知の光量の光を前記光検出手段に与えることで、前記光量算出手段により算出される光量を校正する校正手段と、 By giving to the light detecting means light of a known light intensity by the fluorescent signal serving as the reference, a calibration means for calibrating the amount of light calculated by the light amount calculating means,
    を備えることを特徴とする光量計測装置。 Light amount measuring apparatus comprising: a.
  2. 光検出手段で取り込んだ光の光量を計測する光量計測方法において、 In the light amount measuring method for measuring the amount of light captured by the light detecting means,
    前記光検出手段の出力信号に基づいて蛍光信号光の光量を算出するステップと、 Calculating a light amount of fluorescent signal light based on an output signal of said light detecting means,
    励起光照射手段から基準となる蛍光信号を発生する基準蛍光信号発生手段に励起光を照射するステップと、 A step of irradiating excitation light to a reference fluorescent signal generating means for generating a fluorescent signal as a reference from the excitation light emitting means,
    前記基準となる蛍光信号により既知の光量の光を前記光検出手段に与えることで、前記光量算出手段により算出される光量を校正するステップと、 By giving to the light detecting means light of a known light intensity by the fluorescent signal serving as the reference, the steps of calibrating the light amount calculated by the light amount calculating means,
    を備えることを特徴とする光量計測方法。 Light amount measuring method characterized by comprising a.
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JPH10311789A (en) * 1997-05-13 1998-11-24 Nikon Corp Photometer
JP2003524754A (en) * 1998-05-16 2003-08-19 ピーイー コーポレイション (エヌワイ) Apparatus for monitoring a polymerase chain reaction Dna
WO2004018623A2 (en) * 2002-08-16 2004-03-04 Clinical Microarrays, Inc. Substrates for isolating, reacting and microscopically analyzing materials
WO2004019019A1 (en) * 2002-08-21 2004-03-04 Amersham Biosiciences Uk Limited Fluorescence reference plate
JP2005515473A (en) * 2002-01-18 2005-05-26 ニユートン・ラボラトリーズ・インコーポレーテツド Spectroscopic diagnostic methods and systems

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03120445A (en) * 1989-10-04 1991-05-22 Hitachi Ltd Automatic fluorescence intensity measuring instrument
JPH10311789A (en) * 1997-05-13 1998-11-24 Nikon Corp Photometer
JP2003524754A (en) * 1998-05-16 2003-08-19 ピーイー コーポレイション (エヌワイ) Apparatus for monitoring a polymerase chain reaction Dna
JP2005515473A (en) * 2002-01-18 2005-05-26 ニユートン・ラボラトリーズ・インコーポレーテツド Spectroscopic diagnostic methods and systems
WO2004018623A2 (en) * 2002-08-16 2004-03-04 Clinical Microarrays, Inc. Substrates for isolating, reacting and microscopically analyzing materials
JP2005535909A (en) * 2002-08-16 2005-11-24 ディシジョン バイオマーカーズ インコーポレイテッド Substrate for the separation of materials, reaction, and microscopic analysis
WO2004019019A1 (en) * 2002-08-21 2004-03-04 Amersham Biosiciences Uk Limited Fluorescence reference plate
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