JP2011010616A - Container for treating adipose tissue - Google Patents

Abstract

PROBLEM TO BE SOLVED: To smoothly perform the treatment of an adipose tissue by sufficiently discharging only tumescent liquid.SOLUTION: This container 1 for treating the adipose tissue includes a container body 2 housing the adipose tissue A sucked out from a body and tumescent liquid B and having a discharging port 2b opening up at a bottom surface 2a, piping 4 connected with the discharging port 2b and discharging fluid from the inside of the container body 2, a filter 3 covering the discharging port 2b in the container body 2 and having a hole having a smaller diameter dimension than that of the adipose tissue A, detecting parts 5a, 5b for detecting transmittance at the inside of the piping 4 in the vicinity of the discharging port 2b, and a controlling part 7 stopping the discharge from the discharging port 2b based on the change of the transmittance detected by the detecting parts 5a, 5b during the discharge of the fluid.

Description

  The present invention relates to an adipose tissue processing container.

Conventionally, an apparatus for separating fat-derived cells such as stem cells from adipose tissue collected from the body is known (see, for example, Patent Document 1). The collected fat-derived cells are transplanted into a patient's body and used for regenerative medicine, for example.
In addition, as a method for collecting adipose tissue, a tumescent solution in which a hemostatic agent or an anesthetic is added to physiological saline is injected into the adipose tissue site, and the adipose tissue is swollen with the tumescent solution and loosened softly, and then these are simultaneously aspirated. A tumecent method is known (for example, see Non-Patent Document 1).

JP 2005-519883 Gazette

Worrisome Medicine-Liposuction / Lipolysis Injection, Tumescnet, [online], [Search January 26, 2009], Internet <http://www.k-igaku.com/liposuction/term_105 .html>

  When adipose tissue is collected by the tumecent method, impurities such as blood that are simultaneously aspirated from the body need to be washed and removed. However, in order to reduce the number and time of washing and improve the efficiency of the work, the aspiration tissue was aspirated. It is essential to first sufficiently remove the portion separated from the adipose tissue in the tumecent liquid. However, since the adipose tissue swells by sucking the tumecent liquid, the amount of the tumecent liquid discharged is reduced compared to the amount injected, and the amount of the tumecent liquid sucked by the adipose tissue varies depending on the adipose tissue. Therefore, the actual discharge amount is not known in advance.

  Accordingly, there is a problem in that when the tumecent liquid is discharged by the amount injected into the body, the discharged amount becomes excessive and adipose tissue is also discharged. At this time, if the container is provided with a filter for preventing the discharge of adipose tissue, the adipose tissue is excessively sucked and clogged in the hole of the filter or pressed against the filter and closely adhered. For this reason, there are problems such as that it cannot be smoothly discharged in a subsequent processing step, and that when the cleaning liquid is injected, the adipose tissue is in close contact with the filter and does not re-float in the cleaning liquid and cannot be cleaned normally. Furthermore, if the discharge is continued, the container may be damaged.

  The present invention has been made in view of the above-described circumstances, and provides a fat tissue processing container capable of sufficiently discharging a tumecent liquid while leaving only a fat tissue and smoothly processing the fat tissue. It is an object.

In order to achieve the above object, the present invention provides the following means.
The present invention contains a container body having a discharge port opened at the bottom, containing adipose tissue and a tumecent liquid sucked from the body, a pipe connected to the discharge port and discharging the liquid from the container body, A filter that covers the discharge port in the container body and has a hole with a diameter smaller than that of the fat tissue, a detection unit that detects the transmittance in the pipe in the vicinity of the discharge port, and the detection during the discharge of the liquid And a control unit that stops discharge from the discharge port based on a change in transmittance detected by the unit.

  According to the present invention, when the adipose tissue and the tumecent liquid are separated into layers by the difference in specific gravity in the container body and discharged from the outlet, the tumecent liquid separated into the lower layer and the middle layer, respectively, and the fat content contained in the adipose tissue Among them, the liquefied fat is sequentially discharged, and the upper layer fatty tissue that does not pass through the filter remains in the container body, and the fat tissue can be subsequently processed in the container body.

  In this case, when the tumecent liquid containing red blood cells or the like collected simultaneously from the body is discharged from the discharge port, light is absorbed by the red blood cells and the like, so that a low transmittance is detected by the detection unit. Then, after the tubular liquid and the liquefied fat are discharged, a space is created between the fat tissue blocked by the filter and the discharge port, and air enters the pipe, thereby increasing the transmittance.

  Therefore, according to the present invention, the control unit stops the discharge after detecting that the tumescent liquid and the liquefied fat are actually discharged, so that the tubent liquid and the liquefied fat are contained in the container body. Can be fully discharged. In addition, since the drainage is stopped in a sufficiently short time after the tumescent liquid and the liquefied fat are discharged from the container body, the fat tissue is excessively sucked and clogged in the filter hole or adhered to the filter. It is prevented from disappearing. Thereby, the process of the fat tissue performed continuously can be advanced smoothly.

In the above invention, the control unit may stop the discharge from the discharge port when the transmittance detected by the detection unit increases at a change rate larger than a predetermined threshold.
Moreover, in the said invention, the said control part is good also as stopping discharge | emission from the said discharge port, when the transmittance | permeability detected by the said detection part rises to a predetermined threshold value.
In this way, it is possible to quickly and accurately determine that the tuccent fluid and the liquefied fat have been sufficiently discharged from the container body and the air has entered the pipe, and the excessive suction of the adipose tissue can be performed in advance. Can be prevented.

In the above invention, the storage unit stores the transmittance in the pipe before discharging the liquid, and the control unit stores the transmittance detected by the detection unit in the storage unit. The discharge from the discharge port may be stopped when the transmittance increases.
By doing so, the discharge is stopped when the inside of the pipe is sufficiently filled with air and the state is the same as the state filled with air or a transparent liquid before discharging the tumecent liquid, The tumecent liquid and the liquefied fat can be sufficiently discharged.

In the above invention, the pipe has a window portion made of a transparent material at a position facing at least the radial direction of the side surface in the vicinity of the discharge port, and the detection section radiates the pipe outside the window section. It is good also as providing the light emission part which is arrange | positioned on both sides, and radiate | emits detection light in the direction of the said window part, and the light-receiving part which light-receives the said detection light which has passed through the said window and the said piping.
In this way, the transmittance in the pipe can be detected from the outside of the pipe in a non-contact manner with the adipose tissue and the tubular liquid.

  According to the present invention, there is an effect that the treatment of the adipose tissue can be performed smoothly by leaving only the adipose tissue and sufficiently discharging the tumecent liquid.

1 is an overall configuration diagram of an adipose tissue processing container according to an embodiment of the present invention. It is a graph which shows the result of having measured the transmittance | permeability in piping when discharging | emitting a tumecent liquid using the fat tissue processing container of FIG.

An adipose tissue processing container 1 according to an embodiment of the present invention will be described below with reference to FIGS. 1 and 2.
As shown in FIG. 1, the adipose tissue processing container 1 according to the present embodiment includes a container body 2 having a bottom surface 2a with an outlet 2b, a filter 3 provided in the container body 2, and an outlet 2b. Connected to the pipe 4, a photoelectric sensor (detector, hereinafter also referred to as photoelectric sensors 5a and 5b) provided in the middle of the pipe 4 and having a light emitting part 5a and a light receiving part 5b, and connected to the light receiving part 5b. A storage unit 6 and a control unit 7 are provided.

  The container body 2 has a cylindrical shape, and the upper part is closed by a lid 2c. The lid 2c is provided with a suction port 2d to which a cannula or the like for sucking the adipose tissue A from the body is connected, and an injection port 2e for injecting a washing liquid for washing the collected adipose tissue A. Adipose tissue A and tumescent fluid B are accommodated. Further, the bottom surface 2a of the container body 2 is inclined in one direction, and a discharge port 2b is opened at the lowest position of the bottom surface 2a.

The filter 3 has a semicircular shape having substantially the same diameter as the inner diameter of the container main body 2, and is arranged horizontally with a clearance between the inner surface of the container main body 2 on the lower side of the bottom surface 2 a so that the discharge port 2 b is provided. Covering. Further, the filter 3 has a hole having a diameter smaller than that of the fat tissue A, and prohibits the passage of the fat tissue A.
The pipe 4 is provided in the vicinity of the discharge port 2b at a position where a window 4a made of a highly transparent material such as glass faces in the radial direction. The pipe 4 is connected to a liquid feed pump 8.

The light emitting unit 5a and the light receiving unit 5b of the photoelectric sensor are arranged outside the window 4a with the pipe 4 interposed in the radial direction. The detection light L emitted from the light emitting unit 5a toward the light receiving unit 5b passes through the window 4a and the pipe 4 in the radial direction and is received by the light receiving unit 5b, whereby the transmittance in the pipe 4 is detected. It is like that.
Further, the light receiving unit 5 b detects the intensity of the received detection light L as a voltage and outputs information on the voltage value to the control unit 7. Note that the intensity and voltage value of the detection light L are proportional to each other.

  When the adipose tissue A and the tumescent fluid B are allowed to stand in the container main body 2, the adipose tissue A is contained in the upper layer due to the difference in specific gravity, and the tumescent fluid B containing impurities such as blood is contained in the lower layer and the adipose tissue A. Of the fat, liquefied fat C is separated into the middle layer. When the liquid feed pump 8 is operated in this state, it is discharged from the lower outlet tubular liquid B first through the outlet 2b and passes through the position of the detection light L.

  At this time, since the detection light L is absorbed by red blood cells or the like contained in the tubular liquid B, the transmittance is greatly reduced as compared with air or a transparent liquid. Further, when the discharge is continued and the tubular liquid B and the liquefied fat C are removed from the container body 2, the adipose tissue A is held at a position away from the discharge port 2 b by the filter 3. Penetrates and the transmittance increases rapidly.

The storage unit 6 stores an initial state before the tubular liquid B is discharged from the discharge port 2b and a voltage value of the detection light L detected by the light receiving unit 5b during the discharge.
The controller 7 starts the discharge from the container body 2 and the voltage value sequentially input from the light receiving unit 2b is smaller than a predetermined ratio with respect to the initial voltage value stored in the storage unit 6. Then, when the voltage value sequentially stored in the storage unit 6 rises at a rate of change exceeding a predetermined threshold, the liquid feed pump 8 is stopped.

The operation of the adipose tissue processing container 1 configured as described above will be described below.
In order to process the adipose tissue A using the adipose tissue processing container 1 according to the present embodiment, as a pretreatment, a transparent cleaning liquid is injected into the container body 2 from the injection port 2e and then discharged from the discharge port 2b. The inside of the container body 2 and the pipe 4 is cleaned. Then, the voltage value of the detection light L detected by the light receiving unit 5 b in a state where the cleaning liquid is filled in the pipe 4 is stored in the storage unit 6.

  Next, a cannula or the like is connected to the suction port 2d, and the adipose tissue A and the tubular liquid B are collected by the tubular method and accommodated in the container body 2, and these are separated into layers. Then, when the liquid feeding pump 8 is operated, the liquid feeding pump 8 is stopped at the time when the liquefied fat C is sufficiently discharged after the lower layer of the tumecent liquid B is discharged. The adipose tissue A from which the liquid B has been removed is left in the container body 2. Subsequently, when a treatment liquid such as a washing liquid is injected into the container body 2 from the injection port 2e, the adipose tissue A can be processed.

  FIG. 2 is a graph showing the results of measuring the time change of the transmittance in the pipe 4 when the tumecent liquid B is discharged in the vicinity of the discharge port 2b, and the horizontal axis indicates the discharge time from the start of discharge, The vertical axis represents the voltage value of the detection light L detected by the light receiving unit 5b. The vertical axis represents the ratio to the voltage value (initial voltage value) detected when the cleaning liquid is injected during the pretreatment.

  Immediately after the discharge is started, the cleaning liquid remaining in the pipe 4 passes through the position of the detection light L, so that a high transmittance is maintained, and the tumescent liquid B discharged from the discharge port 2b passes after about 20 seconds. Thus, the transmittance is reduced by about 80%. When about 150 seconds elapse, the liquefied fat C subsequently passes through the Tuccent liquid B, and the transmittance further decreases, and then the transmittance rapidly increases due to the passage of air (FIG. 2). (See the arrow of.)

  As described above, according to the present embodiment, by stopping the discharge when the transmittance once decreases to a predetermined ratio with respect to the initial state and then increases at a change rate exceeding a predetermined threshold, There is an advantage that the discharge of the liquefied fat C can be actually detected and the discharge can be surely continued until these are sufficiently removed from the container body 2. Moreover, there is an advantage that the adipose tissue A can be efficiently cleaned by sufficiently removing the tumecent liquid B from the container body 2 in this way.

  Further, when the control unit 7 stops the discharge based on the change rate of the transmittance, it is quickly detected that the tumecent liquid B and the liquefied fat C are sufficiently discharged and the air enters the pipe 4. The discharge is stopped. As a result, the adipose tissue A is excessively aspirated and clogged in the pores of the filter 3, making it difficult to discharge, or the adipose tissue A is in close contact with the filter 3 and does not re-float in the cleaning solution and cannot be washed normally. There is an advantage that the processing of the adipose tissue A can be continued and proceeded smoothly. Further, it is possible to prevent damage such as deformation of the container body 2 due to excessive suction of the inside of the container body 2 due to continued suction from the discharge port 2b.

  In addition, by providing the pipe 4 with the window 4a, the photoelectric sensors 5a and 5b can be arranged outside the pipe 4 so that the transmittance can be measured without contact with the adipose tissue A and the tumescent liquid B. It is possible to replace only the container main body 2 and the pipe 4 other than 5a and 5b using disposable ones.

  Further, the pipe 4 and the photoelectric sensors 5a and 5b are exchanged by detecting the intensity of the received detection light L as a voltage and detecting the discharge of the tumecent liquid B based on the rate of decrease with respect to the initial voltage value. Even if the measurement conditions change due to the difference or state of these solids, it is possible to accurately detect the discharge of the tubular liquid B every time.

In the above embodiment, the control unit 7 stops the liquid feeding pump 8 when the transmittance increases at a rate of change larger than the predetermined threshold. Instead, the transmittance is set to a predetermined threshold. It is good also as stopping the liquid feeding pump 8 when exceeding.
In the case of FIG. 2, the tumecent liquid B has a transmittance of about 20% with respect to the cleaning liquid. Therefore, by setting the threshold value to, for example, 30% of the voltage value of the cleaning liquid, it is discharged in a sufficiently short time after these are discharged while sufficiently discharging the chucent liquid B and the liquefied fat C. Can be stopped.

DESCRIPTION OF SYMBOLS 1 Adipose tissue processing container 2 Container body 2a Bottom surface 2b Discharge port 2c Lid 2d Suction port 2e Inlet 3 Filter 4 Piping 4a Window (window part)
5a Photoelectric sensor, light emitting part (detection part)
5b Photoelectric sensor, light receiving part (detection part)
6 Storage Unit 7 Control Unit 8 Liquid Feed Pump A Adipose Tissue B Tumescent Solution C Liquefied Fat Content L Detection Light

Claims (5)

A container main body that contains adipose tissue and a tumecent liquid sucked from the body and has a discharge port opened in the bottom surface;
A pipe connected to the outlet and for discharging liquid from the container body;
A filter that covers the outlet in the container body and has a hole with a smaller diameter than the fat tissue;
A detector for detecting the transmittance in the pipe in the vicinity of the outlet;
A fat tissue processing container comprising: a control unit that stops discharge from the discharge port based on a change in transmittance detected by the detection unit during discharge of the liquid.   2. The adipose tissue processing container according to claim 1, wherein the control unit stops the discharge from the discharge port when the transmittance detected by the detection unit increases at a change rate larger than a predetermined threshold.   The adipose tissue processing container according to claim 1, wherein the control unit stops the discharge from the discharge port when the transmittance detected by the detection unit rises to a predetermined threshold value. A storage unit for storing the transmittance in the pipe before discharging the liquid;
2. The adipose tissue processing container according to claim 1, wherein the control unit stops discharging from the discharge port when the transmittance detected by the detecting unit rises to the transmittance stored in the storage unit. The pipe has a window portion made of a transparent material at a position facing at least the radial direction of the side surface near the discharge port,
The detection part is disposed outside the window part with the pipe interposed in the radial direction, and emits detection light in the direction of the window part, and the detection light that has passed through the window and the pipe. The adipose tissue processing container according to claim 1, further comprising a light receiving unit that receives light.

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