JP2010502962A - 電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置 - Google Patents
電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置 Download PDFInfo
- Publication number
- JP2010502962A JP2010502962A JP2009526932A JP2009526932A JP2010502962A JP 2010502962 A JP2010502962 A JP 2010502962A JP 2009526932 A JP2009526932 A JP 2009526932A JP 2009526932 A JP2009526932 A JP 2009526932A JP 2010502962 A JP2010502962 A JP 2010502962A
- Authority
- JP
- Japan
- Prior art keywords
- well
- collection
- electrophoresis
- loading
- cassette
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 373
- 238000000034 method Methods 0.000 title claims abstract description 270
- 239000000499 gel Substances 0.000 title abstract description 259
- 230000005684 electric field Effects 0.000 claims abstract description 128
- 239000000872 buffer Substances 0.000 claims abstract description 57
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims description 97
- 238000000926 separation method Methods 0.000 claims description 83
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 36
- 238000010367 cloning Methods 0.000 claims description 35
- 102000004169 proteins and genes Human genes 0.000 claims description 31
- 238000012544 monitoring process Methods 0.000 claims description 30
- 108090000623 proteins and genes Proteins 0.000 claims description 30
- 229920000936 Agarose Polymers 0.000 claims description 22
- 238000011282 treatment Methods 0.000 claims description 20
- 239000012634 fragment Substances 0.000 claims description 18
- 229920002401 polyacrylamide Polymers 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000012545 processing Methods 0.000 claims description 9
- 239000012146 running buffer Substances 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 6
- 238000004949 mass spectrometry Methods 0.000 claims description 5
- 230000000712 assembly Effects 0.000 abstract description 17
- 238000000429 assembly Methods 0.000 abstract description 17
- 239000000523 sample Substances 0.000 description 146
- 238000012800 visualization Methods 0.000 description 35
- 239000011159 matrix material Substances 0.000 description 30
- 150000007523 nucleic acids Chemical class 0.000 description 26
- 108020004414 DNA Proteins 0.000 description 24
- 239000000975 dye Substances 0.000 description 23
- 108020004707 nucleic acids Proteins 0.000 description 23
- 102000039446 nucleic acids Human genes 0.000 description 23
- 238000002955 isolation Methods 0.000 description 22
- 102000004196 processed proteins & peptides Human genes 0.000 description 22
- 150000001413 amino acids Chemical class 0.000 description 21
- 239000011324 bead Substances 0.000 description 20
- 239000000126 substance Substances 0.000 description 18
- 239000002245 particle Substances 0.000 description 16
- 108091034117 Oligonucleotide Proteins 0.000 description 15
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
- 239000000203 mixture Substances 0.000 description 13
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 12
- 230000000670 limiting effect Effects 0.000 description 12
- -1 Cassettes Substances 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 10
- 229920001184 polypeptide Polymers 0.000 description 10
- 230000003851 biochemical process Effects 0.000 description 9
- 230000002441 reversible effect Effects 0.000 description 9
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 8
- 238000013508 migration Methods 0.000 description 8
- 230000005012 migration Effects 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 230000035620 dolor Effects 0.000 description 7
- 229920000642 polymer Polymers 0.000 description 7
- 102000053602 DNA Human genes 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000009423 ventilation Methods 0.000 description 6
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 108010024636 Glutathione Proteins 0.000 description 5
- 230000031018 biological processes and functions Effects 0.000 description 5
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 5
- 229960005542 ethidium bromide Drugs 0.000 description 5
- 239000007850 fluorescent dye Substances 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 4
- 108010038807 Oligopeptides Proteins 0.000 description 4
- 102000015636 Oligopeptides Human genes 0.000 description 4
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
- 230000003196 chaotropic effect Effects 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- 238000005342 ion exchange Methods 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 239000012474 protein marker Substances 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 238000000378 two-dimensional separation method Methods 0.000 description 4
- 238000005349 anion exchange Methods 0.000 description 3
- 238000003491 array Methods 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000005341 cation exchange Methods 0.000 description 3
- 230000002860 competitive effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 229960003180 glutathione Drugs 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 3
- 238000007794 visualization technique Methods 0.000 description 3
- QZTKDVCDBIDYMD-UHFFFAOYSA-N 2,2'-[(2-amino-2-oxoethyl)imino]diacetic acid Chemical compound NC(=O)CN(CC(O)=O)CC(O)=O QZTKDVCDBIDYMD-UHFFFAOYSA-N 0.000 description 2
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- ACERFIHBIWMFOR-UHFFFAOYSA-N 2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)azaniumyl]propane-1-sulfonate Chemical compound OCC(C)(C)NCC(O)CS(O)(=O)=O ACERFIHBIWMFOR-UHFFFAOYSA-N 0.000 description 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 2
- RZQXOGQSPBYUKH-UHFFFAOYSA-N 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCC(CO)(CO)NCC(O)CS(O)(=O)=O RZQXOGQSPBYUKH-UHFFFAOYSA-N 0.000 description 2
- XCBLFURAFHFFJF-UHFFFAOYSA-N 3-[bis(2-hydroxyethyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCCN(CCO)CC(O)CS(O)(=O)=O XCBLFURAFHFFJF-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 108010074860 Factor Xa Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 description 2
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical compound NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 229920002319 Poly(methyl acrylate) Polymers 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229910002804 graphite Inorganic materials 0.000 description 2
- 239000010439 graphite Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000011133 lead Substances 0.000 description 2
- 239000004973 liquid crystal related substance Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000007769 metal material Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- 229920005615 natural polymer Polymers 0.000 description 2
- 229910052759 nickel Inorganic materials 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052763 palladium Inorganic materials 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 229920000139 polyethylene terephthalate Polymers 0.000 description 2
- 239000005020 polyethylene terephthalate Substances 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 229920001059 synthetic polymer Polymers 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 description 1
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 1
- AJTVSSFTXWNIRG-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid Chemical compound OCC[NH+](CCO)CCS([O-])(=O)=O AJTVSSFTXWNIRG-UHFFFAOYSA-N 0.000 description 1
- 125000000954 2-hydroxyethyl group Chemical group [H]C([*])([H])C([H])([H])O[H] 0.000 description 1
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 description 1
- 239000007991 ACES buffer Substances 0.000 description 1
- 108700016232 Arg(2)-Sar(4)- dermorphin (1-4) Proteins 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
- 150000008574 D-amino acids Chemical class 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 description 1
- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical compound OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 description 1
- GIZQLVPDAOBAFN-UHFFFAOYSA-N HEPPSO Chemical compound OCCN1CCN(CC(O)CS(O)(=O)=O)CC1 GIZQLVPDAOBAFN-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 150000008575 L-amino acids Chemical class 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
- 239000007997 Tricine buffer Substances 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 208000034953 Twin anemia-polycythemia sequence Diseases 0.000 description 1
- 108010006886 Vitrogen Proteins 0.000 description 1
- 150000001241 acetals Chemical class 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
- 239000007998 bicine buffer Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 108010053098 biotin receptor Proteins 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 210000001520 comb Anatomy 0.000 description 1
- 238000000748 compression moulding Methods 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 239000000112 cooling gas Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 1
- OGGXGZAMXPVRFZ-UHFFFAOYSA-M dimethylarsinate Chemical compound C[As](C)([O-])=O OGGXGZAMXPVRFZ-UHFFFAOYSA-M 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 238000004049 embossing Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- TWYVVGMYFLAQMU-UHFFFAOYSA-N gelgreen Chemical compound [I-].[I-].C1=C(N(C)C)C=C2[N+](CCCCCC(=O)NCCCOCCOCCOCCCNC(=O)CCCCC[N+]3=C4C=C(C=CC4=CC4=CC=C(C=C43)N(C)C)N(C)C)=C(C=C(C=C3)N(C)C)C3=CC2=C1 TWYVVGMYFLAQMU-UHFFFAOYSA-N 0.000 description 1
- JGBUYEVOKHLFID-UHFFFAOYSA-N gelred Chemical compound [I-].[I-].C=1C(N)=CC=C(C2=CC=C(N)C=C2[N+]=2CCCCCC(=O)NCCCOCCOCCOCCCNC(=O)CCCCC[N+]=3C4=CC(N)=CC=C4C4=CC=C(N)C=C4C=3C=3C=CC=CC=3)C=1C=2C1=CC=CC=C1 JGBUYEVOKHLFID-UHFFFAOYSA-N 0.000 description 1
- 239000011544 gradient gel Substances 0.000 description 1
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 150000002460 imidazoles Chemical class 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012806 monitoring device Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 239000012811 non-conductive material Substances 0.000 description 1
- 229910052755 nonmetal Inorganic materials 0.000 description 1
- 238000003499 nucleic acid array Methods 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- SCUZVMOVTVSBLE-UHFFFAOYSA-N prop-2-enenitrile;styrene Chemical compound C=CC#N.C=CC1=CC=CC=C1 SCUZVMOVTVSBLE-UHFFFAOYSA-N 0.000 description 1
- 238000003498 protein array Methods 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002342 ribonucleoside Substances 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000010979 ruby Substances 0.000 description 1
- 229910001750 ruby Inorganic materials 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 235000009518 sodium iodide Nutrition 0.000 description 1
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 description 1
- 229910001488 sodium perchlorate Inorganic materials 0.000 description 1
- UYCAUPASBSROMS-AWQJXPNKSA-M sodium;2,2,2-trifluoroacetate Chemical compound [Na+].[O-][13C](=O)[13C](F)(F)F UYCAUPASBSROMS-AWQJXPNKSA-M 0.000 description 1
- YCLWMUYXEGEIGD-UHFFFAOYSA-M sodium;2-hydroxy-3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonate Chemical compound [Na+].OCCN1CCN(CC(O)CS([O-])(=O)=O)CC1 YCLWMUYXEGEIGD-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 229920000638 styrene acrylonitrile Polymers 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003856 thermoforming Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Electrostatic Separation (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82421006P | 2006-08-31 | 2006-08-31 | |
| US82951706P | 2006-10-13 | 2006-10-13 | |
| PCT/US2007/077383 WO2008028127A1 (en) | 2006-08-31 | 2007-08-31 | Methods, cassettes, gels and apparatuses for isolation and collection of biomolecules from electrophoresis gels |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010502962A true JP2010502962A (ja) | 2010-01-28 |
| JP2010502962A5 JP2010502962A5 (https=) | 2011-05-26 |
Family
ID=39136297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009526932A Pending JP2010502962A (ja) | 2006-08-31 | 2007-08-31 | 電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置 |
Country Status (4)
| Country | Link |
|---|---|
| US (3) | US20080057557A1 (https=) |
| EP (1) | EP2062036A4 (https=) |
| JP (1) | JP2010502962A (https=) |
| WO (1) | WO2008028127A1 (https=) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014517314A (ja) * | 2011-06-16 | 2014-07-17 | ブリティッシュ コロンビア キャンサー エイジェンシー ブランチ | 核酸の自動サイズ選択 |
| JP2020008513A (ja) * | 2018-07-12 | 2020-01-16 | 株式会社日立ハイテクノロジーズ | 電気泳動方法、電気泳動システム及び電気泳動ゲル |
| WO2023002775A1 (ja) * | 2021-07-19 | 2023-01-26 | 株式会社ベックス | 電極および電極キット |
| US12467897B2 (en) | 2020-09-02 | 2025-11-11 | Hitachi High-Tech Corporation | Electrophoresis device and biological substance recovery method |
| US12578304B2 (en) | 2019-06-20 | 2026-03-17 | Hitachi High-Tech Corporation | Method for recovering biological substance and device for recovering biological substance |
| US12590924B2 (en) | 2021-06-28 | 2026-03-31 | Hitachi High-Tech Corporation | Electrophoresis apparatus and electrophoresis method |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009057794A1 (ja) * | 2007-10-31 | 2009-05-07 | Arkray, Inc. | コントロール液の判別方法および分析装置 |
| US8361299B2 (en) * | 2008-10-08 | 2013-01-29 | Sage Science, Inc. | Multichannel preparative electrophoresis system |
| AU2009302248B2 (en) * | 2008-10-08 | 2014-03-06 | Sage Science, Inc. | Multichannel preparative electrophoresis system |
| TWI358539B (en) * | 2008-12-09 | 2012-02-21 | Univ Nat Taiwan | Integrated electrophoresis device and operation th |
| JP5304352B2 (ja) * | 2009-03-13 | 2013-10-02 | 凸版印刷株式会社 | ろ紙を不要とした転写装置 |
| US20100326827A1 (en) * | 2009-06-24 | 2010-12-30 | Shang-Chi Lin | Method and apparatus for isolating and purifying bio-molecules |
| WO2011025781A1 (en) * | 2009-08-24 | 2011-03-03 | Life Technologies Corporation | Gel electrophoresis, imaging, and analysis methods, devices, systems, and materials |
| JP2012029676A (ja) * | 2010-07-07 | 2012-02-16 | Sony Corp | 核酸濃縮回収用カートリッジ、核酸濃縮回収方法、及び該カートリッジの製造方法 |
| US9599590B2 (en) | 2012-10-12 | 2017-03-21 | Sage Science, Inc. | Side-eluting molecular fractionator |
| GB2517864B (en) * | 2013-03-07 | 2015-12-09 | Univ California | Electrophoretic separation devices and methods for using the same |
| CN103555799B (zh) * | 2013-10-26 | 2015-09-23 | 吉林大学 | 一种免疫活性肽冻干粉及其制备方法 |
| WO2016061416A1 (en) * | 2014-10-15 | 2016-04-21 | Sage Science, Inc. | Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation |
| EP3353538B1 (en) | 2015-09-22 | 2024-04-10 | Bio-Rad Laboratories, Inc. | Electrophoresis receptacle and method |
| WO2017087979A1 (en) | 2015-11-20 | 2017-05-26 | Washington University | Preparative electrophoretic method for targeted purification of genomic dna fragments |
| JP2020515250A (ja) | 2017-04-07 | 2020-05-28 | セージ サイエンス， インコーポレイテッド | 統合型電気泳動ｄｎａ精製を使用する遺伝的構造変動を検出するためのシステムおよび方法 |
| CN109470760B (zh) * | 2018-12-26 | 2024-05-31 | 天康生物制药有限公司 | 用于制备琼脂糖凝胶的装置、琼脂糖凝胶电泳方法和应用 |
| KR102250148B1 (ko) * | 2019-11-12 | 2021-05-10 | 주식회사 티맥 | 수평형 전기영동장치 |
| DE102020109087A1 (de) * | 2020-04-01 | 2021-10-07 | MobiCron GmbH | Elektrophoresevorrichtung zur Verwendung bei einem Electroclearing-Verfahren |
| US20220099619A1 (en) * | 2020-09-30 | 2022-03-31 | Icare Diagnostics International Co. Ltd. | Electrode and application thereof |
| CN113607714B (zh) * | 2021-10-08 | 2022-01-11 | 成都齐碳科技有限公司 | 分子膜成膜或表征器件、装置、方法以及生物芯片 |
| EP4419901A4 (en) * | 2021-10-21 | 2025-11-19 | Zadeh Cameron O | HIGH-SPEED MULTIPLEXED ELECTROPHORETIC GEL APPARATUS AND ASSOCIATED PROCESSES |
| WO2025233688A1 (en) | 2024-05-08 | 2025-11-13 | Axor Biosystems Inc. | Microplate-based electrophoresis system |
| CN118547047A (zh) * | 2024-05-29 | 2024-08-27 | 苏州中析生物信息有限公司 | 一种核酸分离纯化方法及分离纯化装置 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63222254A (ja) * | 1987-03-12 | 1988-09-16 | Kusano Hiroshi | 電気泳動装置用回収セル |
| JP2004155687A (ja) * | 2002-11-05 | 2004-06-03 | Forestry & Forest Products Research Institute | 等電点電気泳動法によるタンパク質溶出方法。 |
| JP2004294317A (ja) * | 2003-03-27 | 2004-10-21 | Matsushita Electric Ind Co Ltd | 生体物質回収装置 |
| JP2004290109A (ja) * | 2003-03-27 | 2004-10-21 | Matsushita Electric Ind Co Ltd | 生体物質回収装置 |
| JP2004309326A (ja) * | 2003-04-08 | 2004-11-04 | Yokogawa Electric Corp | 生体高分子の抽出方法および抽出装置 |
| JP2005241477A (ja) * | 2004-02-26 | 2005-09-08 | Japan Science & Technology Agency | 電気泳動用ゲルおよびその利用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1530326A (en) * | 1923-01-04 | 1925-03-17 | Harry A Prindle | Centrifugal pump |
| US2827268A (en) * | 1953-12-07 | 1958-03-18 | Staaf Gustaf Adolf | Liquid transporting apparatus |
| US3512375A (en) * | 1968-11-27 | 1970-05-19 | Sealectro Corp | Flexible coupling for shafts |
| US3856272A (en) * | 1972-06-08 | 1974-12-24 | Richards Of Rockford Inc | Floating mixer |
| US4030859A (en) * | 1975-09-02 | 1977-06-21 | Lake Aid Inc. | Floating aerator having means to vary the length of the draft pipe |
| US4179243A (en) * | 1977-06-21 | 1979-12-18 | Aide Richard J | Floatation pump device |
| US4151065A (en) * | 1978-01-30 | 1979-04-24 | The Regents Of The University Of California | Horizontal slab gel electrophoresis |
| US4764313A (en) * | 1986-11-03 | 1988-08-16 | Sunset Solar Systems Ltd. | Air driven water circulation mill |
| US5139637A (en) * | 1991-03-13 | 1992-08-18 | Macconnell William P | Plasmid purification system and method |
| US5217593A (en) * | 1991-03-13 | 1993-06-08 | Macconnell William P | Nucleic acid purification system and method |
| US5384022A (en) * | 1993-09-08 | 1995-01-24 | Cornell Research Foundation, Inc. | Method and apparatus for electrophoretic DNA band isolation |
| US6379516B1 (en) * | 1995-04-26 | 2002-04-30 | Ethrog Biotechnology Ltd. | Apparatus and method for electrophoresis |
| US5785835A (en) * | 1996-04-12 | 1998-07-28 | One Lambda | Electrophoresis method and devices |
| US5800691A (en) * | 1996-12-23 | 1998-09-01 | Guest Elchrom Scientific Ag | Electrophoresis gels containing sample wells with enlarged loading area |
| US6146511A (en) * | 1998-01-30 | 2000-11-14 | The Perkin-Elmer Corporation | Electrophoretic nucleic acid purification method |
| US6521111B1 (en) * | 2000-04-10 | 2003-02-18 | Invitrogen Corporation | Methods and articles for labeling polymer gels |
| US6562213B1 (en) * | 2000-08-30 | 2003-05-13 | Ethrog Biotechnology Ltd. | Electrophoresis apparatus for simultaneous loading of multiple samples |
| US6602391B2 (en) * | 2001-03-05 | 2003-08-05 | Vladimir B. Serikov | Apparatus and method for combined capillary separation and blotting of biological macromolecules |
| US6632340B2 (en) * | 2001-03-23 | 2003-10-14 | Bio-Rad Laboratories, Inc. | Precast gel and tray combination for submerged gel electrophoresis |
| WO2005033342A1 (en) * | 2003-09-30 | 2005-04-14 | Molecular Probes, Inc. | Detection of immobilized nucleic acid |
-
2007
- 2007-08-31 JP JP2009526932A patent/JP2010502962A/ja active Pending
- 2007-08-31 US US11/848,412 patent/US20080057557A1/en not_active Abandoned
- 2007-08-31 EP EP07841717.7A patent/EP2062036A4/en not_active Withdrawn
- 2007-08-31 WO PCT/US2007/077383 patent/WO2008028127A1/en not_active Ceased
-
2008
- 2008-10-28 US US12/259,823 patent/US20090145759A1/en not_active Abandoned
-
2012
- 2012-07-16 US US13/550,332 patent/US20130020199A1/en not_active Abandoned
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63222254A (ja) * | 1987-03-12 | 1988-09-16 | Kusano Hiroshi | 電気泳動装置用回収セル |
| JP2004155687A (ja) * | 2002-11-05 | 2004-06-03 | Forestry & Forest Products Research Institute | 等電点電気泳動法によるタンパク質溶出方法。 |
| JP2004294317A (ja) * | 2003-03-27 | 2004-10-21 | Matsushita Electric Ind Co Ltd | 生体物質回収装置 |
| JP2004290109A (ja) * | 2003-03-27 | 2004-10-21 | Matsushita Electric Ind Co Ltd | 生体物質回収装置 |
| JP2004309326A (ja) * | 2003-04-08 | 2004-11-04 | Yokogawa Electric Corp | 生体高分子の抽出方法および抽出装置 |
| JP2005241477A (ja) * | 2004-02-26 | 2005-09-08 | Japan Science & Technology Agency | 電気泳動用ゲルおよびその利用 |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014517314A (ja) * | 2011-06-16 | 2014-07-17 | ブリティッシュ コロンビア キャンサー エイジェンシー ブランチ | 核酸の自動サイズ選択 |
| US9952176B2 (en) | 2011-06-16 | 2018-04-24 | British Columbia Cancer Agency Branch | Automated size selection of nucleic acids |
| JP2020008513A (ja) * | 2018-07-12 | 2020-01-16 | 株式会社日立ハイテクノロジーズ | 電気泳動方法、電気泳動システム及び電気泳動ゲル |
| WO2020012805A1 (ja) * | 2018-07-12 | 2020-01-16 | 株式会社日立ハイテクノロジーズ | 電気泳動方法、電気泳動システム及び電気泳動ゲル |
| US11493477B2 (en) | 2018-07-12 | 2022-11-08 | Hitachi High-Tech Corporation | Electrophoresis method, electrophoresis system, and electrophoresis gel |
| US12578304B2 (en) | 2019-06-20 | 2026-03-17 | Hitachi High-Tech Corporation | Method for recovering biological substance and device for recovering biological substance |
| US12467897B2 (en) | 2020-09-02 | 2025-11-11 | Hitachi High-Tech Corporation | Electrophoresis device and biological substance recovery method |
| US12590924B2 (en) | 2021-06-28 | 2026-03-31 | Hitachi High-Tech Corporation | Electrophoresis apparatus and electrophoresis method |
| WO2023002775A1 (ja) * | 2021-07-19 | 2023-01-26 | 株式会社ベックス | 電極および電極キット |
| JPWO2023002775A1 (https=) * | 2021-07-19 | 2023-01-26 | ||
| JP7329295B2 (ja) | 2021-07-19 | 2023-08-18 | 株式会社ベックス | 電極および電極キット |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2062036A4 (en) | 2016-09-21 |
| EP2062036A1 (en) | 2009-05-27 |
| US20090145759A1 (en) | 2009-06-11 |
| WO2008028127A1 (en) | 2008-03-06 |
| US20080057557A1 (en) | 2008-03-06 |
| US20130020199A1 (en) | 2013-01-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2010502962A (ja) | 電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置 | |
| US8361299B2 (en) | Multichannel preparative electrophoresis system | |
| EP1421373B1 (en) | Apparatus and method for separating an analyte | |
| US6562213B1 (en) | Electrophoresis apparatus for simultaneous loading of multiple samples | |
| US8926817B2 (en) | Programmable electrophoretic notch filter systems and methods | |
| JP6141989B2 (ja) | 側方溶出型分子分画装置 | |
| US20100126862A1 (en) | Multichannel preparative electrophoresis system | |
| EP2184602A1 (en) | Micro-channel chip for electrophoresis and method for electrophoresis | |
| EP2986978B1 (en) | Mini-gel comb | |
| EP3695905A1 (en) | Molecular separation by diffusion using an ewod device | |
| US10710079B2 (en) | Electro-kinectic device for species exchange | |
| US20050072674A1 (en) | Method and device for introducing a sample into an electrophoretic apparatus | |
| US20030087290A1 (en) | Bio-affinity porous matrix in microfluidic channels | |
| Kowalski et al. | High-resolution two-dimensional polyacrylamide gel electrophoresis: a tool for identification of polymorphic and modified linker histone components | |
| JP2012058039A (ja) | キャピラリー電気泳動法による試料の分析方法、キャピラリー電気泳動装置及びキャピラリー電気泳動用チップ | |
| WO2025111055A1 (en) | Systems, devices, and methods for performing preparative electrophoresis | |
| KR20170000603U (ko) | 다중채널 파이펫 사용이 가능한 유전자 전기영동용 아가로즈 겔 | |
| IL190930A (en) | Electrophoresis apparatus for simultaneous loading of multiple samples |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100426 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20100426 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110330 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110921 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20111215 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20111222 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20120517 |