JP2010229111A - Hyaluronic acid production promoter - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a hyaluronic acid production promoter which promotes the hyaluronic acid production ability in humans to increase the amount of hyaluronic acid, wherein the promoter can be used safely and simply, and to provide a hyaluronic acid production promoter suitably used for an external preparation for skin, food and drink, an oral formulation, and the like. <P>SOLUTION: The hyaluronic acid production promoter includes a plant body of Phyllanthus emblica (Emblica officinale) or its extract. Furthermore, the promoter may contain collagen peptide and/or a plant body of Zingiberaceae mango ginger (Curcuma amada ROXB.) or its extract. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a hyaluronic acid production promoter containing a plant of Phyllanthus emblica or Emblica officinale or an extract thereof as an active ingredient. More specifically, the present invention relates to a hyaluronic acid production promoter that promotes hyaluronic acid production ability in humans. The hyaluronic acid production promoter can be effectively applied to prevent aging of human skin (skin elasticity and elasticity retention), prevention / treatment of arthritis, etc., initial treatment of burns, and the like.

  In recent years, research on aging has been promoted. As for the cause of skin aging, aging is an important factor when viewed macroscopically, but in addition to that, the effects of drying, oxidation, sunlight (ultraviolet rays) and the like are also directly related to skin aging. As specific phenomena of skin aging, reduction of mucopolysaccharides including hyaluronic acid, cross-linking reaction of collagen, and damage of cells by ultraviolet rays are known.

  Among them, hyaluronic acid retains moisture in the cell gap, retains cells based on the formation of a jelly-like matrix in the tissue, retains the lubricity and flexibility of the tissue, and resists external forces such as mechanical failure. It has many functions such as prevention of bacterial infection. For example, it is said that the amount of hyaluronic acid in the skin decreases with aging, and accompanying this, skin aging such as fine wrinkles and roughness appears. For this reason, many cosmetics containing hyaluronic acid and collagen have been proposed as an ameliorating agent for such aging skin. However, these conventional cosmetics only exhibit a moisturizing effect on the skin surface, and cannot essentially improve aging skin. In addition, cosmetics containing various vitamins and herbal medicines as skin cell activators have been proposed, but these have not yet been able to improve and treat aging skin.

  Furthermore, the hyaluronic acid contained in the joint fluid covers the surface of the articular cartilage and is useful for smooth operation of the joint function. The concentration of hyaluronic acid in normal human joint fluid is about 2.3 mg / mL, but in the case of rheumatoid arthritis, the concentration of hyaluronic acid in joint fluid decreases to about 1.2 mg / mL and the viscosity of joint fluid is also reduced. Remarkably reduced (Non-Patent Document 1). In addition, it is known that hyaluronic acid content decreases in pyogenic arthritis and gouty arthritis as in the case of rheumatoid arthritis (Non-patent Document 2). In the above diseases, it is conceivable to increase the amount of hyaluronic acid in synovial fluid in order to improve lubrication function, cover / protect articular cartilage, suppress pain, and improve or normalize pathological joint fluid. For example, it has been reported that the above improvement is observed when a joint injection therapy with sodium hyaluronate is performed on a patient with rheumatoid arthritis (Non-patent Document 3). Similarly, in traumatic arthritis, osteoarthritis and osteoarthritis, the above-mentioned improvement effect has been reported by joint injection therapy with hyaluronic acid. (Non-Patent Document 4).

  However, treatment of the above diseases is long-lasting and requires a doctor's prescription. Therefore, an external preparation for skin containing a hyaluronic acid production promoter that can be easily treated in daily life has been desired.

  In the healing process after burn injury, hyaluronic acid is known to increase significantly in the granulation during the period from the beginning of the granulation tissue growing from below the necrotic tissue until the entire tissue is replaced with granulation tissue. (Non-patent Document 5), hyaluronic acid production promoters are also expected as an early treatment for burns.

  On the other hand, drugs that produce hyaluronic acid in human cells include cytokines such as insulin-like growth factor-1, epidermal growth factor (Non-patent document 6) and interleukin-1 (Non-patent document 7), or phorbol esters ( Non-Patent Document 8) and the like are known, but none of them can be used simply and safely as cosmetics, bathing agents, pharmaceuticals and the like.

  As will be described later, the present invention is a technique using an Amla extract, but Amla extract has so far been an MMP activity inhibitor (Patent Document 1), a blood flow improving agent (Patent Document 2), and a collagen production promoter. (Patent Document 3), use in testosterone-5α-reductase inhibitor (Patent Document 4) and the like have been reported. However, as far as the present inventors know, it has never been known at all that Amla extract has an effect of promoting hyaluronic acid production and has an action of positively increasing the amount of hyaluronic acid in human cells. JP 2003-300244 A (Patent Document 5) describes that philanthus emblica (= Amura) has a hyaluronidase activity inhibitory action and suppresses the degradation of hyaluronic acid. Inhibition (= enzyme activity inhibition) is completely different from the action of promoting the hyaluronic acid production action and positively promoting the increase of the amount of hyaluronic acid, and is not related to both actions.

  In addition to the above, as a document describing techniques related to the present invention, JP-A No. 2004-123637 (Patent Document 6) describes that a collagen peptide has a hyaluronic acid production promoting effect. However, the present inventors have confirmed that the amla extract has an extremely superior hyaluronic acid production effect as compared with the collagen peptide, as shown in the Examples section below. Further, when a mango ginger extract known to have a collagen synthesis promoting action, a tyrosinase inhibiting action or the like is further combined with hyaluronic acid as described in JP-A-2005-104886 (Patent Document 7), etc. It was also confirmed that the production promoting effect is synergistically increased. The promotion of hyaluronic acid production in the present invention means an increase in the amount of hyaluronic acid.

JP 2006-282561 A JP 2006-335708 A JP 2008-156287 A JP 2005-145902 A Japanese Patent Laid-Open No. 2003-300824 Japanese Patent Laid-Open No. 2004-123637 JP 2005-104886 A

"Arthritis Rheumatism", vol.10, p.357 (1967) “Combined Composition”, Kanehara Publishing, 481, 1984 "Inflammation", Japanese Society for Inflammation, 11, 16, 1991 "Connective tissue and diseases", Kodansha, p. 246, 1980 "Connective tissue and diseases", Kodansha, 153, 1980 "Biochemica Biophysica Acta", 1014, p.305 (1989) “Japan Society of Obstetrics and Gynecology” magazine, 41, 1943, 1989 "Experimental Cell Research", vol.148, p.377 (1983)

  The present invention has been made in view of the above circumstances, and provides a hyaluronic acid production promoter that promotes hyaluronic acid production ability in humans and increases the amount of hyaluronic acid, which can be used safely and conveniently. Objective. The hyaluronic acid production promoter of the present invention can be effectively applied to prevention of human skin aging (skin elasticity and elasticity retention), prevention / treatment of arthritis, initial treatment of burns, and the like. In particular, the formulation for hyaluronic acid production promoter provides an external preparation for skin that can maintain the firmness and elasticity of the skin, prevent wrinkles, and maintain a moist and youthful skin state.

  As a result of investigating hyaluronic acid production promoting ability of various substances for solving the above-mentioned problems, the present inventors have found that amla or an extract thereof has a very excellent hyaluronic acid production promoting action.

  That is, according to this invention, the hyaluronic acid production promoter containing the plant body of Amla (Phyllanthus emblica or Emblica officinale) or its extract is provided.

  The present invention also provides the hyaluronic acid production promoter further comprising a collagen peptide and / or a plant body of Curcuma amada ROXB. Or an extract thereof.

  The hyaluronic acid production promoter of the present invention has an excellent hyaluronic acid production promoting action, and is suitable for prevention of human skin aging (skin elasticity and elasticity retention), prevention / treatment of arthritis, etc., initial treatment of burns, etc. It can be applied effectively. In particular, by blending the hyaluronic acid production promoter of the present invention with an external preparation for skin (including pharmaceuticals, quasi-drugs, and cosmetics), the production of hyaluronic acid, which is one of the extracellular matrix components, is promoted. It has the effect of maintaining the firmness and elasticity of the skin to prevent wrinkles and maintaining a moist and youthful skin condition.

It is a graph which shows the result of the hyaluronic acid production promotion effect in an Example.

  Hereinafter, the present invention will be described in detail.

  Amla (Phyllanthus emblica or Emblica officinale) used in the present invention is a deciduous sub-tree belonging to the genus Euphorbiaceae, and is distributed from India to the Malaysian region and southern China, and India is considered the origin. Amla has a unique name depending on each region or language, and is also called, for example, citrus citrus, oil citrus, ammarok, malach, Indian gooseberry, and the like.

  Amla can be used raw or dried, but it is preferably used as a dry powder or a solvent extract from the viewpoints of usability and formulation.

  The use site of Amla is not particularly limited, and may be arbitrarily used such as fruits (immature fruits, ripe fruits, dried fruits), leaves, tuberous roots, flowers, seeds and the like. Of these, fruits are preferably used. The Amla extract can be obtained by a conventional method. For example, Amla is dried if necessary, then immersed in an extraction solvent for a certain period of time, or contacted with an extraction solvent that is heated to reflux, and then filtered and concentrated. Can be obtained. As the extraction solvent, any solvent that is usually used for extraction can be used. For example, water, methanol, ethanol, propylene glycol, 1,3-butylene glycol, glycerol and other alcohols, chloroform, dichloroethane, four Organic solvents such as carbon chloride, acetone and ethyl acetate can be used alone or in combination. The extract obtained by extraction with the above solvent is used as it is, or concentrated extracts are used, or these extracts are removed by using an adsorption method, for example, ion exchange resin, or a porous polymer (for example, Amberlite XAD- After adsorbing on the column of 2), elution with methanol or ethanol and concentration can also be used. Further, a partitioning method, for example, an extract extracted with water / ethyl acetate can be used.

  The Amla extract thus obtained is highly safe and has an excellent hyaluronic acid production promoting action. It has not been known at all that Amla and its extract have a hyaluronic acid production promoting action, and it has been confirmed for the first time by the present inventor that these actions can be obtained. Amla extract is also marketed, for example as "San Amla" from Taiyo Kagaku Co., Ltd., and these commercially available products may be used.

  The hyaluronic acid production promoter of the present invention may be used in combination with other components such as collagen peptides, mango ginger, etc., as well as when it consists of Amla plants or extracts thereof. The present inventors have confirmed that when combined with these components, the hyaluronic acid production promoting effect is synergistically improved and the amount of hyaluronic acid is greatly increased.

  Collagen peptide refers to a product obtained by degrading collagen by enzymatic treatment to lower the molecular weight. Collagen that is a starting material for the collagen peptide is not particularly limited, and any of collagens of type I to XIII can be used, and a mixed collagen that is a mixture of these can also be used. Examples of collagen sources include fish (eg, flounder, salmon, sardines, tuna, sharks, etc.), animals (eg, cows, pigs, birds, whales, etc.) and the like. Collagen extraction / purification can be carried out using generally known methods. Specifically, for example, after pulverizing a tissue containing collagen such as bone, skin, tendon, meat, duck, scale, etc., washing with water, extraction with a dilute salt solution, extraction with an acid or alkali solution, pepsin, trypsin or hyaluronidase It can be obtained by purifying collagen by extraction with an enzyme such as salt, and applying known purification means such as salting out and dialysis. It can also be obtained as “regenerated collagen” by a generally known method. Commercially available collagen can also be used as a starting material for collagen peptides. Gelatin is a water-soluble protein obtained by heating and extracting the aforementioned collagen with water. In the present invention, gelatin produced by a generally known method can be used as a starting material for the above-described collagen peptide, and a commercially available product can also be used. A collagen peptide having a molecular weight of about 500 to 5,000 is preferably used. Collagen peptides are commercially available, for example, as “Iquos HDL-50DR” (manufactured by Nitta Gelatin Co., Ltd.), “Marine Collagen DF” (manufactured by Chisso Corp.), etc., and these commercially available products may be used.

  Mango ginger (Curcuma amada ROXB.) Is a plant belonging to the genus Turmeric of Ginger family, and is an annual herb that is distributed or cultivated in the wild in India. Mango ginger (C. amada) can be used raw or dried, but it is preferably used as a dry powder or a solvent extract from the viewpoints of usability and formulation.

  Mango ginger can be used at any site such as whole grass, leaves, tuberous roots, flowers, seeds and the like. Of these, the tuberous root is preferably used. The extraction method is not particularly limited, and can be carried out according to the method described in the above Amla extraction method. Mango ginger extract is commercially available, for example, as “Mango Ginger Dry Extract F” (manufactured by Maruzen Pharmaceutical Co., Ltd.), and these commercially available products may be used.

  When the above components are combined and combined, it is preferable that Amla and any of collagen peptide and mango ginger are combined and mixed at a ratio of approximately 1:10 to 10: 1 (mass ratio, dry mass), more preferably 1: 5-5: 1. In the case of a three-component system, it is preferable to use a combination as appropriate in the above-mentioned combination ratio.

  The hyaluronic acid production promoter of the present invention can be blended with a topical skin preparation and used for humans and animals, and can be blended with various foods and drinks, feeds (such as pet food) and ingested. It can also be administered to humans and animals as a pharmaceutical formulation.

  When mix | blending the amla of this invention with a skin external preparation, the compounding quantity (dry mass) of an amla extract is 0.0001-70 mass% in the external preparation whole quantity, More preferably, it is 0.001-30 mass%. is there. Furthermore, when compounding collagen and a mango ginger in combination, it is preferable to mix | blend so that a total amount may be about 0.0001-70 mass%.

  When the hyaluronic acid production promoter of the present invention is applied to an external preparation for skin, in addition to the above components, it is usually used for an external preparation for skin such as cosmetics and pharmaceuticals as long as it does not impair the effects of the present invention. Ingredients such as antioxidants, oils, UV protection agents, surfactants, thickeners, alcohols, powder components, coloring materials, aqueous components, water, various skin nutrients, etc. may be appropriately blended as necessary. it can.

  Furthermore, edetate disodium, edetate trisodium, sodium citrate, sodium polyphosphate, sodium metaphosphate, sequestering agents such as gluconic acid, preservatives such as methylparaben, ethylparaben, butylparaben, caffeine, tannin, Verapamil, tranexamic acid and its derivatives, licorice extract, grabrizine, hot water extract of karin fruit, various herbal medicines, tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, Whitening agents such as ascorbic acid glucoside, arbutin, and kojic acid, and sugars such as glucose, fructose, mannose, sucrose, and trehalose can be appropriately blended.

  The external preparation for skin can be applied to cosmetics, quasi-drugs, etc., particularly preferably cosmetics applied to the outer skin, and the dosage form can also be applied to the skin. Any dosage form such as solution system, solubilization system, emulsification system, powder dispersion system, water-oil two-layer system, water-oil-powder three-layer system, ointment, lotion, gel, aerosol, etc. is applicable. Is done.

  The use form is also arbitrary, and for example, it can be used for facial cosmetics such as lotions, emulsions, creams and packs, makeup cosmetics such as foundations, lipsticks and eye shadows, aromatic cosmetics, bath preparations and the like.

  In addition, makeup cosmetics can be widely applied to foundations and the like, and toiletries such as body soaps and soaps. Furthermore, if it is a quasi-drug, it can be widely applied to various ointment forms. And the form which the hyaluronic acid production promoter of this invention can take is not limited to these dosage forms and forms.

  When the hyaluronic acid production promoter of the present invention is blended in foods and drinks, feeds, etc., the blending amount (dry mass) of the amla extract can be appropriately determined according to the type, purpose, form, usage method, and the like. For example, it can be made into about 0.1-90 mass% in food-drinks whole quantity. In particular, when used as a health food or drink, it is preferable to contain the active ingredient of the present invention in such an amount that a predetermined effect is sufficiently exhibited.

  As a form of food-drinks or feed, it can be arbitrarily shaped into, for example, granules, granules, pastes, gels, solids, or liquids. These include various known substances that are recognized to be contained in foods and drinks, such as binders, disintegrants, thickeners, dispersants, reabsorption accelerators, flavoring agents, buffers, and surfactants. Excipients such as solubilizers, preservatives, emulsifiers, tonicity agents, stabilizers and pH adjusters may be included as appropriate.

  When the hyaluronic acid production promoter of the present invention is used as a pharmaceutical preparation, the preparation is appropriately used orally or parenterally (intravenous administration, intraperitoneal administration, etc.). The dosage form is also arbitrary, for example, oral solid preparations such as tablets, granules, powders and capsules, oral liquid preparations such as internal liquids and syrups, and parenteral liquid preparations such as injections. These forms can also be appropriately prepared by known methods. For these pharmaceutical preparations, commonly used binders, disintegrants, thickeners, dispersants, reabsorption accelerators, corrigents, buffers, surfactants, solubilizers, preservatives, emulsifiers, isotonicity Excipients such as agents, stabilizers and pH adjusters may be used as appropriate.

  When the hyaluronic acid production promoter of the present invention is used as an external preparation for skin, foods and drinks, feeds, pharmaceutical preparations, etc., it is used for treatment, prevention, improvement, etc. of various symptoms, diseases, pathologies and the like related to a decrease in the amount of hyaluronic acid. Useful. As specific application examples, for example, it is suitably used for prevention of human skin aging (skin elasticity and elasticity retention), prevention / treatment of arthritis, etc., initial treatment of burns, and the like. In addition, with the concept of physiological functions such as treatment, prevention, and improvement of the above symptoms, diseases, and pathological conditions, it should be applied to external preparations for skin, functional foods, foods for the sick, foods for specified health use, etc. Can do.

  EXAMPLES Hereinafter, although this invention is demonstrated further in detail based on an Example, this invention is not limited to a following example.

1. Preparation of samples The following samples were prepared and used as test samples.
-Amla extract: "Sun Amla" manufactured by Taiyo Kagaku Co., Ltd. was used.
Collagen peptide: A commercially available fish-derived collagen hydrolyzate (average molecular weight of 5000 or less) was used.
-Amla extract + collagen peptide: The sample which mixed the said amla extract and collagen peptide in the ratio of 1: 1 (mass ratio) was used.
Amla extract + collagen peptide + mango ginger extract: Amla extract, collagen peptide, and mango ginger extract (oil obtained by extracting mango ginger rootstock with ethanol and evaporating the solvent) A sample mixed at a ratio of 1: 1 (mass ratio) was used.

2. Measurement of hyaluronic acid content 1. The hyaluronic acid production promoting action of each sample obtained in the above was evaluated. That is, when human dermal fibroblasts were seeded in a 24-well plate at a concentration of 5.0 × 10 ⁴ cells / well and brought to a confluent state, the above-mentioned 1. 500 μL of DMEM (0.5% FBS) culture solution containing each sample group (final concentration 5 μg / mL) was added. After the addition, after 72 hours of incubation, the amount of hyaluronic acid produced was measured by ELISA. A DMSO / PBS solution (no sample added) was used as a control example (control). For the measurement, a Hyaluronan assay kit (manufactured by Seikagaku Biobusiness) was used. The results are shown in FIG. In the figure, the vertical axis represents the production amount (ng / mL) of hyaluronan (synonymous with hyaluronic acid).

  As is clear from the results in FIG. 1, the amount of hyaluronic acid in the cells cultured in the Amla extract-containing medium was significantly increased compared to the amount of hyaluronic acid in the control cells. That is, it was confirmed that Amla extract has a very excellent hyaluronic acid production promoting effect. The collagen peptide was almost the same as the control example (in FIG. 1, it is shown as a slightly lower graph than the control example, but this is due to measurement error). In addition, in the sample in which the collagen peptide was combined with the amla extract and the sample in which the mango ginger was further combined therewith, the hyaluronic acid production promoting effect was synergistically improved as compared with the case of amla alone.

  The test (measurement of hyaluronic acid amount) method is usually used as a method for evaluating the hyaluronic acid production promoting effect, and is not a method for evaluating the hyaluronidase degradation inhibitory effect. In the evaluation of the hyaluronidase degradation inhibitory effect, the hyaluronic acid amount is 1.5 times or more (vs. control) in a short period of 72 hours as described above, and no significant increase in hyaluronic acid amount can be considered. If this is not involved, such a result cannot be obtained.

  An example of blending formulation is shown below. In the following, “POE” means “polyoxyethylene”. The amount of each plant extract is dry mass.

[Formulation Example 1: Candy]
2000mg sugar
Minamata 1926mg
Pearl extract 2mg
Lotus germ extract 2mg
Hyaluronic acid production promoter: Amla extract (dry mass) 30mg
Fragrance 40mg
(Total) 4000mg

[Formulation Example 2: Tablet]
Sucrose ester 70mg
Crystalline cellulose 74mg
Methylcellulose 36mg
Glycerin 25mg
Winged bean extract (dry weight) 300mg
Collagen peptide 90mg
Pearl barley extract 10mg
Hyaluronic acid production promoter: Amla extract (dry mass) 90mg
N-acetylglucosamine 180mg
Hyaluronic acid 150mg
Vitamin E 30mg
Vitamin B ₆ 20mg
Vitamin B ₂ 10mg
α-Lipoic acid 20mg
Coenzyme Q10 40mg
Ceramide (konjac extract) 55mg
L-proline 300mg
(Total) 1500mg

[Formulation Example 3: Soft Capsule A]
Edible soybean oil 528mg
Pearl barley extract (dry mass) 8mg
Lotus germ extract (dry mass) 16mg
Hyaluronic acid production promoter: Amla extract (dry mass) 135mg
Royal Jelly 70mg
Maca 60mg
GABA (= γ-aminobutyric acid) 30 mg
Beeswax 60mg
Gelatin 375mg
Glycerin 113mg
Glycerin fatty acid ester 105mg
(Total) 1500mg

[Formulation Example 4: Soft Capsule B]
Brown rice germ oil 650mg
Hyaluronic acid production promoter:
(Breakdown)
Amla extract (dry mass) 185mg
Mango ginger extract (dry weight) 185mg
Collagen peptide 185mg
Pearl barley extract (dry mass) 50mg
Resveratrol 5mg
Elastin 180mg
DNA 30mg
Folic acid 30mg
(Total) 1500mg

[Formulation 5: Granules]
Hyaluronic acid production promoter:
(Breakdown)
Collagen peptide 70mg
Amla extract (dry mass) 140mg
Pearl barley extract 20mg
Lotus germ extract 40mg
Vitamin C 150mg
Soy isoflavone 270mg
Reduced lactose 360mg
Soybean oligosaccharide 36mg
Erythritol 36mg
Dextrin 30mg
Fragrance 24mg
Citric acid 24mg
(Total) 1200mg

[Composition Example 6: Drink (50 mL)]
Hyaluronic acid production promoter 15mg
(Breakdown)
Mango ginger extract (dry weight) 5mg
Collagen peptide 5mg
Amla extract (dry mass) 5mg
Pearl barley extract (dry mass) 2mg
Reduced maltose starch syrup 28mg
Erythritol 8mg
Citric acid 2mg
Fragrance 1.3mg
N-acetylglucosamine 1mg
Hyaluronic acid 0.5mg
Vitamin E 0.3mg
α-Lipoic acid 0.2mg
Coenzyme Q10 1.2mg
Water residue

[Formulation example 7: serum]
(Compounding ingredients) (mass%)
(Phase A)
95% ethyl alcohol 10.0
POE (20) Octyldodecanol 1.0
Pantothenyl ethyl ether 0.1
Hyaluronic acid production promoter: Amla extract (dry mass) 1.5
Methylparaben 0.15
(Phase B)
Potassium hydroxide 0.1
(Phase C)
Glycerin 5.0
Dipropylene glycol 10.0
Sodium bisulfite 0.03
Carboxyvinyl polymer 0.2
Purified water residue

[Formulation Example 8: Pack]
(Compounding ingredients) (mass%)
(Phase A)
Dipropylene glycol 5.0
Polyoxyethylene (60 mol) hydrogenated castor oil 5.0
(Phase B)
Hyaluronic acid production promoter:
(Breakdown)
Amla extract (dry mass) 0.01
Collagen peptide 0.01
Olive oil 5.0
Tocopherol acetate 0.2
Ethylparaben 0.2
Fragrance 0.2
(Phase C)
Sodium bisulfite 0.03
Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2000) 13.0
Ethanol 7.0
Purified water residue

[Formulation Example 9: Emulsion]
(Compounding ingredients) (mass%)
Microcrystalline wax 1.0
Beeswax 2.0
Lanolin 20.0
Liquid paraffin 10.0
Squalane 5.0
Sorbitan sesquioleate ester 4.0
POE (20) sorbitan monooleate 1.0
Propylene glycol 7.0
Hyaluronic acid production promoter:
(Breakdown)
Amla extract (dry mass) 10.0
Collagen peptide 1.0
Mango ginger extract (dry weight) 5.0
Sodium bisulfite 0.01
Ethylparaben 0.3
Perfume Appropriate amount Purified water Residual

[Formulation Example 10: Cream]
(Compounding ingredients) (mass%)
Solid paraffin 5.0
Beeswax 10.0
Vaseline 15.0
Glycerin monostearate ester 2.0
POE (20) sorbitan monolaurate 3.0
Soap powder 0.1
Borax 0.2
Hyaluronic acid production promoter: Amla extract (dry mass) 0.05
Sodium bisulfite 0.03
Ethylparaben 0.3
Perfume Appropriate amount Purified water Residual

  The present invention provides a hyaluronic acid production promoter that is safe, has no side effects even after long-term use, can be used easily, and is extremely excellent in increasing the amount of hyaluronic acid produced.

Claims (2)

  A hyaluronic acid production promoter comprising a plant of Amla (Phyllanthus emblica or Emblica officinale) or an extract thereof.   Furthermore, the hyaluronic acid production promoter of Claim 1 containing the plant body of collagen peptide and / or the ginger family mango ginger (Curcuma amada ROXB.), Or its extract.