JP2008522144A5 - - Google Patents
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- Publication number
- JP2008522144A5 JP2008522144A5 JP2007542164A JP2007542164A JP2008522144A5 JP 2008522144 A5 JP2008522144 A5 JP 2008522144A5 JP 2007542164 A JP2007542164 A JP 2007542164A JP 2007542164 A JP2007542164 A JP 2007542164A JP 2008522144 A5 JP2008522144 A5 JP 2008522144A5
- Authority
- JP
- Japan
- Prior art keywords
- matrix
- electrode
- electrophoresis
- dimensional electrophoresis
- plane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001962 electrophoresis Methods 0.000 claims description 17
- 230000005684 electric field Effects 0.000 claims description 13
- 239000011159 matrix material Substances 0.000 claims 43
- 238000000034 method Methods 0.000 claims 26
- 238000000539 two dimensional gel electrophoresis Methods 0.000 claims 24
- 239000012472 biological sample Substances 0.000 claims 12
- 239000000523 sample Substances 0.000 claims 11
- 238000000926 separation method Methods 0.000 claims 7
- 239000000126 substance Substances 0.000 claims 4
- 229920000936 Agarose Polymers 0.000 claims 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 3
- 238000004925 denaturation Methods 0.000 claims 3
- 230000036425 denaturation Effects 0.000 claims 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims 2
- 230000029936 alkylation Effects 0.000 claims 2
- 238000005804 alkylation reaction Methods 0.000 claims 2
- 229920002301 cellulose acetate Polymers 0.000 claims 2
- 239000003638 chemical reducing agent Substances 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 239000004020 conductor Substances 0.000 claims 2
- 239000008151 electrolyte solution Substances 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 239000004033 plastic Substances 0.000 claims 2
- 229920003023 plastic Polymers 0.000 claims 2
- 229920000642 polymer Polymers 0.000 claims 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 claims 2
- KGIGUEBEKRSTEW-UHFFFAOYSA-N 2-vinylpyridine Chemical compound C=CC1=CC=CC=N1 KGIGUEBEKRSTEW-UHFFFAOYSA-N 0.000 claims 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims 1
- 235000003392 Curcuma domestica Nutrition 0.000 claims 1
- 244000008991 Curcuma longa Species 0.000 claims 1
- 229920002307 Dextran Polymers 0.000 claims 1
- -1 N-substituted acrylamide Chemical class 0.000 claims 1
- 239000004677 Nylon Substances 0.000 claims 1
- 238000000376 autoradiography Methods 0.000 claims 1
- 230000004071 biological effect Effects 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 235000003373 curcuma longa Nutrition 0.000 claims 1
- 239000003398 denaturant Substances 0.000 claims 1
- 238000000326 densiometry Methods 0.000 claims 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims 1
- 239000003365 glass fiber Substances 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 238000011065 in-situ storage Methods 0.000 claims 1
- PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 claims 1
- 238000002218 isotachophoresis Methods 0.000 claims 1
- 239000012139 lysis buffer Substances 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 claims 1
- 229920001778 nylon Polymers 0.000 claims 1
- 239000003960 organic solvent Substances 0.000 claims 1
- 229920002401 polyacrylamide Polymers 0.000 claims 1
- 238000006116 polymerization reaction Methods 0.000 claims 1
- 239000004094 surface-active agent Substances 0.000 claims 1
- TUQOTMZNTHZOKS-UHFFFAOYSA-N tributylphosphine Chemical compound CCCCP(CCCC)CCCC TUQOTMZNTHZOKS-UHFFFAOYSA-N 0.000 claims 1
- 235000013976 turmeric Nutrition 0.000 claims 1
- 238000012800 visualization Methods 0.000 claims 1
- 238000007693 zone electrophoresis Methods 0.000 claims 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT000301A ITPD20040301A1 (it) | 2004-11-26 | 2004-11-26 | Metodo ed apparato per la separazione simultanea di molecole biologiche mediante elettroforesi bidimensionale |
| ITPD2004A000301 | 2004-11-26 | ||
| PCT/IB2005/003519 WO2006056861A1 (en) | 2004-11-26 | 2005-11-24 | Method and apparatus for the simultaneous separation of biological molecules by two dimensional electrophoresis |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008522144A JP2008522144A (ja) | 2008-06-26 |
| JP2008522144A5 true JP2008522144A5 (https=) | 2012-03-29 |
| JP5006790B2 JP5006790B2 (ja) | 2012-08-22 |
Family
ID=36124054
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007542164A Expired - Fee Related JP5006790B2 (ja) | 2004-11-26 | 2005-11-24 | ２次元電気泳動による生体分子の同時分離に関する方法及び装置 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20080202934A1 (https=) |
| EP (1) | EP1828760A1 (https=) |
| JP (1) | JP5006790B2 (https=) |
| KR (1) | KR20070089918A (https=) |
| CN (1) | CN101111762B (https=) |
| CA (1) | CA2589232A1 (https=) |
| IT (1) | ITPD20040301A1 (https=) |
| WO (1) | WO2006056861A1 (https=) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| WO2014060483A1 (en) | 2012-10-17 | 2014-04-24 | Spatial Transcriptomics Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| EP3013983B1 (en) | 2013-06-25 | 2023-02-15 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| US10774374B2 (en) | 2015-04-10 | 2020-09-15 | Spatial Transcriptomics AB and Illumina, Inc. | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| CN105498540A (zh) * | 2015-12-08 | 2016-04-20 | 北京大学第一医院 | 一种具有浓缩功能的电泳仪 |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| EP3894590B1 (en) | 2018-12-10 | 2025-10-22 | 10X Genomics, Inc. | Methods of using master / copy arrays for spatial detection |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| CN111560063B (zh) * | 2020-05-12 | 2022-11-01 | 蚌埠医学院 | 一种动物胰脏来源胰岛素原料药纯化装置及使用方法 |
| CN116134308B (zh) | 2020-05-19 | 2025-05-27 | 10X基因组学有限公司 | 电泳盒和仪器 |
| EP4153775B1 (en) | 2020-05-22 | 2024-07-24 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| WO2021252747A1 (en) | 2020-06-10 | 2021-12-16 | 1Ox Genomics, Inc. | Fluid delivery methods |
| EP4491742B1 (en) | 2020-09-18 | 2026-03-04 | 10X Genomics, Inc. | Sample handling apparatus and image registration methods |
| EP4729631A2 (en) | 2020-12-21 | 2026-04-22 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| WO2022178267A2 (en) | 2021-02-19 | 2022-08-25 | 10X Genomics, Inc. | Modular assay support devices |
| EP4582555A3 (en) | 2021-06-03 | 2025-10-22 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| US12553805B2 (en) | 2021-08-02 | 2026-02-17 | 10X Genomics, Inc. | Methods of preserving a biological sample |
| USD1064308S1 (en) | 2021-09-17 | 2025-02-25 | 10X Genomics, Inc. | Sample handling device |
| EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| EP4441711A1 (en) | 2021-12-20 | 2024-10-09 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3844925A (en) * | 1973-07-02 | 1974-10-29 | Center For Blood Res | Molecular fractionation |
| EP0497077B1 (de) * | 1991-01-28 | 1996-07-17 | Ciba-Geigy Ag | Vorrichtung zur Vorbereitung von Proben insbesondere für Analysezwecke |
| US6071394A (en) * | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
| US6207031B1 (en) * | 1997-09-15 | 2001-03-27 | Whitehead Institute For Biomedical Research | Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device |
| IT1309430B1 (it) * | 1999-05-18 | 2002-01-23 | Guerrieri Roberto | Metodo ed apparato per la manipolazione di particelle per mezzo delladielettroforesi |
| ATE359502T1 (de) * | 1999-07-16 | 2007-05-15 | Applera Corp | Vorrichtung und verfahren für hochdichte elektrophorese |
| US6328870B1 (en) * | 2000-01-27 | 2001-12-11 | Cbm Intellectural Properties, Inc. | Electrophoresis gel running plate |
| GB0010957D0 (en) * | 2000-05-05 | 2000-06-28 | Novartis Ag | Compound & method |
| EP1211510A1 (en) * | 2000-11-29 | 2002-06-05 | Proteologics, Inc. | Methods and apparatus for nonlinear mobility electrophoresis separation |
| AUPR222300A0 (en) * | 2000-12-21 | 2001-01-25 | Life Therapeutics Limited | Electrophoresis device and method |
| EP1379864A1 (en) * | 2001-04-17 | 2004-01-14 | Nextgen Sciences Ltd | Electrophoretic separation system |
| GB0130235D0 (en) * | 2001-12-18 | 2002-02-06 | Deltadot Ltd | Centrifugal spectrometer |
| JP3672910B2 (ja) * | 2002-07-12 | 2005-07-20 | 太陽誘電株式会社 | マイクロキャピラリーを有するディスク状光記録媒体、及び該ディスク状光記録媒体を用いた試料分析装置 |
-
2004
- 2004-11-26 IT IT000301A patent/ITPD20040301A1/it unknown
-
2005
- 2005-11-24 EP EP05850670A patent/EP1828760A1/en not_active Withdrawn
- 2005-11-24 CN CN2005800405505A patent/CN101111762B/zh not_active Expired - Fee Related
- 2005-11-24 US US11/791,728 patent/US20080202934A1/en not_active Abandoned
- 2005-11-24 JP JP2007542164A patent/JP5006790B2/ja not_active Expired - Fee Related
- 2005-11-24 KR KR1020077011797A patent/KR20070089918A/ko not_active Abandoned
- 2005-11-24 CA CA002589232A patent/CA2589232A1/en not_active Abandoned
- 2005-11-24 WO PCT/IB2005/003519 patent/WO2006056861A1/en not_active Ceased
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