JP2008239168A - Multiplex sterilized package and method for warehousing sterilized object using the same - Google Patents

Multiplex sterilized package and method for warehousing sterilized object using the same Download PDF

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JP2008239168A
JP2008239168A JP2007078776A JP2007078776A JP2008239168A JP 2008239168 A JP2008239168 A JP 2008239168A JP 2007078776 A JP2007078776 A JP 2007078776A JP 2007078776 A JP2007078776 A JP 2007078776A JP 2008239168 A JP2008239168 A JP 2008239168A
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sterilized
package
sterilization
area
cleanness
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Katsumi Nakajima
勝己 中嶋
Takashi Sakurai
隆 櫻井
Takashi Saeki
尚 佐伯
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Kawasaki Heavy Industries Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/14Bags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus

Abstract

<P>PROBLEM TO BE SOLVED: To provide a multiplex sterilized package for warehousing a sterilized consumable item or the like in a highly clean area while maintaining a high degree of cleanness, and to provide a method for warehousing a sterilized object. <P>SOLUTION: The lid of a pass box 25 is opened, the outer most sterilized bag 5c of a triple sterilized bag 6 is torn and a double sterilized package 6' is placed into the pass box 25. Then, the lid of the pass box is opened from the clean room side, and the double sterilized package is conveyed in it. Subsequently, the door of a glove box 15 is opened, the outermost sterilized bag 5b of the double sterilized package is torn, and a single sterilized package 6'' in it is taken out and placed into the glove box. Then, the process waits until the degree of cleanness in the glove box reaches 100 or below. Thereafter, the single sterilized package is torn using gloves attached to the operation holes 18a and 18b of the glove box, and a truck 23 having thereon a consumable item in the package is conveyed to a communicating zone 16, in which the consumable item on the truck is taken into an automatic cell culture apparatus by an operation robot. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

本発明は、多重滅菌包装物及びこれを用いた滅菌物の入庫方法に関し、更に詳細には、自動細胞培養装置内部等のクリーン度の高いエリアに、滅菌済みの消耗品等を高いクリーン度を保ったまま入庫するための多重滅菌包装物及びこれを用いた滅菌物の入庫方法に関する。   The present invention relates to a multiple sterilized package and a method for storing a sterilized product using the same, and more specifically, a high degree of cleanliness of sterilized consumables and the like in an area with a high degree of cleanness such as the inside of an automatic cell culture apparatus. The present invention relates to a multiple sterilized package for warehousing and a sterilized warehousing method using the same.

自動細胞培養装置内部等はクリーン度の高い状態にあり、ここにピペット用チップ、ディッシュ、遠心管、サンプル管等の消耗品を入庫する際には、菌で汚染されないように特に注意が必要である。従来より、滅菌済みの消耗品は滅菌袋入りの状態で供給されており、これをクリーンベンチに搬入する場合には、滅菌袋にアルコールを噴霧してクリーンベンチ内に入庫し、クリーンベンチ内で滅菌袋から滅菌済みの消耗品を取り出すのが通常である。しかし、アルコール噴霧では滅菌袋に付いた全ての雑菌が除去できるわけではなく、また、作業者によってアルコール噴霧の程度がまちまちで、人的要因により滅が残る可能性がある。   The inside of the automatic cell culture device etc. is in a clean state, and when consumables such as pipette tips, dishes, centrifuge tubes, sample tubes, etc. are stored here, special care must be taken to prevent contamination with bacteria. is there. Conventionally, sterilized consumables have been supplied in a sterilized bag. When transporting this product to a clean bench, the sterilized bag is sprayed with alcohol and stored in the clean bench. It is usual to remove sterilized consumables from the sterilization bag. However, the alcohol spray cannot remove all the germs attached to the sterilization bag, and the level of the alcohol spray varies depending on the worker, and there is a possibility that it may be destroyed by human factors.

この点を考慮し、作業室内で消耗品を過酸化水素蒸気を使用して滅菌することが検討されている(特許文献1)。しかし、この方法では、例えば密閉し得る蓋のある容器では過酸化水素蒸気が容器内に浸入しないため、滅菌を行うことはできない。また、蓋を緩めておけば蒸気は浸入するが、滅菌修了後においても蒸気が容器内に残留するという問題点がある。
特開2005−278565号公報、段落番号〔0007〕
Considering this point, it has been studied to sterilize consumables using hydrogen peroxide vapor in a working chamber (Patent Document 1). However, in this method, for example, in a container having a lid that can be sealed, hydrogen peroxide vapor does not enter the container, so that sterilization cannot be performed. In addition, if the lid is loosened, the vapor enters, but there is a problem that the vapor remains in the container even after the completion of sterilization.
Japanese Patent Laying-Open No. 2005-278565, paragraph number [0007]

本発明は、このような従来技術の問題点を解決するために為されたものであり、本発明の目的は、自動細胞培養装置内部等のクリーン度の高いエリアに、滅菌済みの消耗品等を高いクリーン度を保ったまま入庫するための多重滅菌包装物及びこれを用いた滅菌物の入庫方法を提供することである。   The present invention has been made to solve such problems of the prior art, and the object of the present invention is to provide sterilized consumables, etc. in an area with a high degree of cleanness such as the inside of an automatic cell culture apparatus. Is to provide a multiple sterilized package for warehousing while maintaining a high degree of cleanliness, and a sterilized material warehousing method using the same.

本発明の多重滅菌包装物は、滅菌物を滅菌袋を用いて多重包装した多重滅菌包装物であって、最外部の滅菌袋の外側を除き、各滅菌袋の内側及び外側が滅菌されていることを特徴とする。   The multiple sterilization package of the present invention is a multiple sterilization package in which a sterilized product is packaged in multiples using a sterilization bag, and the inside and outside of each sterilization bag are sterilized except for the outside of the outermost sterilization bag. It is characterized by that.

このように滅菌物を滅菌袋を用いて無菌状態で多重包装した多重滅菌包装物を使用すれば、クリーン度の高いエリアに搬入する直前に最外部の滅菌袋を破って中身を取り出し、これを高いクリーン度のエリアに搬入することができ、滅菌物への菌の付着を極力避けることが可能となる。   In this way, if multiple sterilized packages are used, the sterilized products are packaged aseptically using a sterilized bag, the outermost sterilized bag is broken and the contents are taken out immediately before being brought into a clean area. It can be carried into an area with a high degree of cleanliness, and it is possible to avoid the adhesion of bacteria to sterilized materials as much as possible.

ここで、滅菌物としては、例えば、滅菌済みのピペット用チップ、ディッシュ、遠心管、サンプル管等を挙げることができる。   Here, examples of the sterilized product include sterilized pipette tips, dishes, centrifuge tubes, and sample tubes.

本発明の滅菌物の入庫方法は、クリーン度の低いエリアからクリーン度の高いエリアに滅菌物を移動させて入庫を行う上記多重滅菌包装物を用いた滅菌物の入庫方法であって、前記多重滅菌包装物を収容している所定のクリーン度のエリアにおいて、前記多重滅菌包装物の最外部の滅菌袋から中身を取り出して、クリーン度の高いエリアに搬入することを特徴とする。   The sterilized product storage method of the present invention is a sterilized product storage method using the above-described multiple sterilized package in which a sterilized product is moved from an area with a low cleanness to an area with a high cleanness. In an area of a predetermined cleanliness containing the sterilized package, the contents are taken out from the outermost sterilization bag of the multiple sterilized package and carried into an area with a high cleanliness.

また、上記の方法では、前記所定のクリーン度のエリアにおいて、クリーン度が搬入先のクリーン度の高いエリアのクリーン度と同じになった後に、前記多重滅菌包装物の最外部の滅菌袋から中身を取り出して前記クリーン度の次に高いエリアに搬入してもよい。   Further, in the above method, after the cleanness becomes the same as the cleanness of the area where the cleanliness is high in the destination, the contents from the outermost sterilization bag of the multiple sterilization package are obtained. May be taken out and carried into an area with the next highest cleanliness.

更に、上記何れかの滅菌物の入庫方法を複数回繰り返してもよい。   Furthermore, any of the above-described methods for storing a sterilized product may be repeated a plurality of times.

このように多重滅菌包装物の最外部の滅菌袋を破ってから中身を取り出してクリーン度高いエリアに搬入することにより、滅菌物への菌の付着を極力避けることが可能となる。   In this way, by tearing the outermost sterilization bag of the multiple sterilized package and taking out the contents and carrying it into an area with a high degree of cleanliness, it is possible to avoid the attachment of bacteria to the sterilized product as much as possible.

本発明の多重滅菌包装物を使用し、本発明の滅菌物の入庫方法を実施することにより、自動細胞培養装置内部等のクリーン度の高いエリアに、滅菌済みの消耗品等を高いクリーン度を保ったまま入庫することが可能となる。また、この操作を繰り返し行うことにより、菌で汚染されることなく滅菌物の入庫を行うことが可能となる。しかも、人的要因により菌で汚染させることもない。   By using the multiple sterilized package of the present invention and carrying out the sterilized product storage method of the present invention, a high degree of cleanliness of sterilized consumables, etc. can be obtained in a high clean area such as the inside of an automatic cell culture apparatus. It is possible to enter the warehouse while keeping it. Further, by repeating this operation, it becomes possible to store a sterilized product without being contaminated with bacteria. Moreover, it is not contaminated with bacteria due to human factors.

本発明の実施形態について、図面を参照しながら説明する。図1は本発明の多重滅菌包装物によって包装される消耗品を図式的に例示したものである。本実施形態において、多重滅菌包装物によって包装される消耗品は、ピペット用チップ1、ディッシュ2、遠心管3及びサンプル管4であり、実際に包装されるのは、ピペット用チップ1及びチップ用スタンド1a(同図(a))、ラベル2bを貼付したディッシュ2及びディッシュ用スタンド2a(同図(b))、ラベル3bを貼付した遠心管3及び遠心管用スタンド3a(同図(c))、ラベル4bを貼付したサンプル管4及びサンプル管用スタンド4a(同図(d))である。図1(a)〜(d)に示した包装対象物は、図2に示すように、それぞれ三重に滅菌袋によって包装される。即ち、最も内側の滅菌袋5a、2番目の滅菌袋5b及び最外部の滅菌袋5cにより、三重に包装される。このように三重包装された包装物は、電子線照射、ガンマ線照射等により滅菌されて、それぞれチップ1の三重滅菌包装物6、ディッシュ2の三重滅菌包装物7、遠心管3の三重滅菌包装物8及びサンプル管4の三重滅菌包装物9が得られる。   Embodiments of the present invention will be described with reference to the drawings. FIG. 1 schematically illustrates consumables packaged by the multiple sterilization package of the present invention. In this embodiment, the consumables packaged by the multiple sterilization package are the pipette tip 1, the dish 2, the centrifuge tube 3 and the sample tube 4, and the pipette tip 1 and the tip are actually packaged. Stand 1a (FIG. (A)), dish 2 with label 2b and dish stand 2a (FIG. (B)), centrifuge tube 3 with label 3b and centrifuge tube stand 3a (FIG. (C)) The sample tube 4 and the sample tube stand 4a with the label 4b attached thereto ((d) in the figure). The packaging objects shown in FIGS. 1 (a) to 1 (d) are each wrapped in three sterilized bags as shown in FIG. That is, the innermost sterilization bag 5a, the second sterilization bag 5b, and the outermost sterilization bag 5c are wrapped in triplicate. The three-packed package is sterilized by electron beam irradiation, gamma-ray irradiation, etc., and the chip 1 triple sterilized package 6, the dish 2 triple sterilized package 7, and the centrifuge tube 3 triple sterilized package, respectively. 8 and a triple sterile package 9 of the sample tube 4 is obtained.

図3は本発明の滅菌物の入庫方法が実施されて多重滅菌包装物が搬入される自動細胞培養装置20の模式図である。本実施形態における自動細胞培養装置20は、所定のクリーン度に設定されたクリーンルーム(図示せず)内に設置されて使用されるものであり、機器設置部10内に設置された操作ロボット11と、細胞の培養などを行う上下に配されたインキュベータ部12,12とを備えている。また、自動細胞培養装置20内には、細胞の遠心分離、ピペット装置等の細胞培養に必要な機器を備えた操作部13を備えている。更に、本実施形態では、滅菌済みのピペット用チップ、ディッシュ、遠心管、サンプル管等の入庫を行う入庫部14を備えており、この入庫部14は、グローブボックス15と、連通部16とにより構成されている。   FIG. 3 is a schematic view of an automatic cell culture apparatus 20 in which a sterilized product storage method according to the present invention is carried and multiple sterilized packages are carried. The automatic cell culture device 20 according to the present embodiment is used by being installed in a clean room (not shown) set to a predetermined cleanness level, and an operation robot 11 installed in the device installation unit 10 and And incubator units 12 and 12 arranged above and below for culturing cells and the like. Further, the automatic cell culture device 20 includes an operation unit 13 equipped with devices necessary for cell culture such as cell centrifugation and pipetting. Furthermore, in this embodiment, it is provided with a warehousing part 14 for warehousing of sterilized pipette tips, dishes, centrifuge tubes, sample tubes, etc., and this warehousing part 14 includes a glove box 15 and a communication part 16. It is configured.

図4は入庫部14の詳細を示す斜視図である。入庫部14のグローブボックス15は、ボックス本体19と扉17とを備え、ボックス本体19の前面には、2つの操作穴18a,18bを備えている。操作穴18a,18bには、それぞれグローブボックス15内で無菌的に人手で作業するためのグローブがボックス本体19の内側に向けて取り付けられている(図示せず)。また、ボックス本体19内には、搬入品を搬送する台車23と、台車23が走行するためのレール24とが設けられている。更に、ボックス本体19の上下には、ボックス本体19内にクリーンなエアーを供給するためのフィルター21及び22が取り付けられている。   FIG. 4 is a perspective view showing details of the warehousing unit 14. The glove box 15 of the warehousing unit 14 includes a box main body 19 and a door 17, and the front surface of the box main body 19 includes two operation holes 18 a and 18 b. In the operation holes 18a and 18b, gloves for working aseptically in the glove box 15 are attached to the inside of the box body 19 (not shown). In the box body 19, there are provided a carriage 23 for transporting the incoming goods and a rail 24 for the carriage 23 to travel. Further, filters 21 and 22 for supplying clean air into the box body 19 are attached to the top and bottom of the box body 19.

入庫部14の連通部16は、グローブボックス15内の搬入品を機器設置部10に取り入れるために設けられており、その内部にはボックス本体19から延伸するレール24を備え、連通部16と機器設置部10との間には、扉16aが設けられている。搬入物を載せた台車23がレール24上を走行し、連通部16内で搬送物は操作ロボット11(図1)によって把持され、自動細胞培養装置20内に取り入れられる。   The communication part 16 of the warehousing part 14 is provided in order to take the carry-in goods in the glove box 15 into the equipment installation part 10, and has a rail 24 extending from the box body 19 in the interior, and the communication part 16 and the equipment A door 16 a is provided between the installation unit 10. A carriage 23 on which a load is loaded travels on a rail 24, and the transported object is gripped by the operating robot 11 (FIG. 1) in the communication unit 16 and taken into the automatic cell culture device 20.

図5は、外部からクリーンルーム及び入庫部14を介して自動細胞培養装置20内に三重滅菌包装物内の滅菌物を入庫する本発明の滅菌物の入庫方法の一実施形態を示しており、図6はそのフローチャートを示している。本実施形態では、外部(図示せず)のクリーン度は100,000より大きく、クリーンルーム(図示せず)のクリーン度は100,000以下であり、グローブボックス15内のクリーン度は100,000から100以下にまでクリーンなエアーを供給することにより、下げることができるように構成されている。自動細胞培養装置20内のクリーン度は100である。なお、図5及び図6では、三重滅菌包装物6に包装されているピペット用チップ1を自動細胞培養装置20内に入庫する場合について説明するが、ディッシュ2の三重滅菌包装物7、遠心管3の三重滅菌包装物8及びサンプル管4の三重滅菌包装物9を自動細胞培養装置20内に入庫する場合も同様である。   FIG. 5 shows an embodiment of the sterilized product storage method of the present invention in which the sterilized product in the triple sterilized package is stored in the automatic cell culture apparatus 20 from the outside via the clean room and the storage unit 14. 6 shows the flowchart. In the present embodiment, the cleanness of the outside (not shown) is greater than 100,000, the cleanness of the clean room (not shown) is 100,000 or less, and the cleanliness in the glove box 15 is from 100,000. It can be lowered by supplying clean air to 100 or less. The degree of cleanliness in the automatic cell culture apparatus 20 is 100. 5 and 6, the case where the pipette tip 1 packaged in the triple sterilization package 6 is stored in the automatic cell culture apparatus 20 will be described. The triple sterilization package 7 of the dish 2, the centrifuge tube, and the like. The same applies when the three sterilized packages 8 and the three sterilized packages 9 of the sample tube 4 are stored in the automatic cell culture apparatus 20.

本実施形態では、クリーンルームとその外部との間に、緩衝域としてパスボックス25(図5(a))が設けられている。三重滅菌包装物6のクリーンルーム内への搬入に際しては、まず、パスボックス25の蓋を開け、続いて三重滅菌包装物6の最外部の滅菌袋5cを破り、その中身の二重滅菌包装物6’を取り出す。この二重滅菌包装物6’を即座にパスボックス25内に入れ、蓋を閉める(図6のS1)。ここで、パスボックス25の蓋を開けてから閉めるまでの一連の動作は、二重滅菌包装物6’の最外部の滅菌袋5bへの菌の付着及びクリーンルームへの雑菌の侵入を防止するために、手早く行う必要がある。   In the present embodiment, a pass box 25 (FIG. 5A) is provided as a buffer area between the clean room and the outside thereof. When carrying the triple sterilized package 6 into the clean room, firstly, the lid of the pass box 25 is opened, then the outermost sterilization bag 5c of the triple sterilized package 6 is broken, and the contents of the double sterilized package 6 'Take out. The double sterilized package 6 'is immediately put in the pass box 25 and the lid is closed (S1 in FIG. 6). Here, a series of operations from opening the lid of the pass box 25 to closing it is for preventing bacteria from adhering to the outermost sterilization bag 5b of the double sterilized package 6 'and invasion of germs into the clean room. It is necessary to do it quickly.

次に、二重滅菌包装物6’を搬入すべきクリーン度の高いクリーンルーム側からパスボックス25の扉を開け、パスボックス25内のクリーン度が100,000以下になれば、図5(b)に示すように、クリーンルーム内に二重滅菌包装物6’を搬入する(S3)。なお、クリーンルーム内への二重滅菌包装物6’の搬入に先だち、紫外線照射を行う場合もある(S2)。   Next, when the door of the pass box 25 is opened from the clean room side where the double sterilized package 6 'is to be carried in, and the clean degree in the pass box 25 becomes 100,000 or less, FIG. As shown in FIG. 3, the double sterilized package 6 ′ is carried into the clean room (S3). In some cases, ultraviolet irradiation may be performed prior to carrying the double sterilized package 6 'into the clean room (S2).

次に、図5(c)に示すように、グローブボックス15の扉17(図4)を開け、続いて二重滅菌包装物6’の最外部の滅菌袋5bを破り、その中身の一重滅菌包装物6”を取り出してグローブボックス15内に即座に収容するとともに、扉17を即座に閉める(S4)。この時点では、グローブボックス15内のクリーン度はクリーンルーム内のクリーン度とほぼ同じレベルである。ここで、扉17を開けてから閉めるまでの一連の動作は、グローブボックス15内及び一重滅菌包装物6”の滅菌袋5aへの菌の付着をできる限り抑制するため、手早く行う必要がある。次に、グローブボックス15内にクリーンなエアーを供給し、グローブボックス15内のクリーン度が100以下になるまで待機する(S5)。   Next, as shown in FIG. 5 (c), the door 17 (FIG. 4) of the glove box 15 is opened, then the outermost sterilization bag 5b of the double sterilized package 6 ′ is broken, and the contents are single sterilized. The package 6 "is taken out and immediately stored in the glove box 15, and the door 17 is immediately closed (S4). At this time, the cleanliness in the glovebox 15 is almost the same as the cleanliness in the clean room. Here, a series of operations from opening the door 17 to closing it must be performed quickly in order to suppress as much as possible the adhesion of bacteria in the glove box 15 and the sterilized bag 5a of the single sterilized package 6 ''. is there. Next, clean air is supplied into the glove box 15 and waits until the cleanliness in the glove box 15 becomes 100 or less (S5).

次に、グローブボックス15内のクリーン度が100以下になれば、図5(d)に示すように、グローブボックス15の操作穴18a,18b(図4)に取り付けたグローブを用いて一重滅菌包装物6”の包装を破り、内部のピペット用チップ1及びチップ用スタンド1aを台車23に載せる(S6)。ピペット用チップ1及びチップ用スタンド1aを載せた台車23は連通部16に搬送される。操作ロボット11は台車23上のチップ用スタンド1aを把持し、自動細胞培養装置20内に取り込むことにより(S7)、ピペット用チップ1の入庫が完了する。   Next, when the cleanliness in the glove box 15 becomes 100 or less, as shown in FIG. 5 (d), single sterilization packaging is performed using the gloves attached to the operation holes 18a and 18b (FIG. 4) of the glove box 15. The packaging of the object 6 ″ is broken and the internal pipette tip 1 and the tip stand 1a are placed on the carriage 23 (S6). The carriage 23 carrying the pipette tip 1 and the tip stand 1a is conveyed to the communicating portion 16. The operating robot 11 grasps the chip stand 1a on the carriage 23 and takes it into the automatic cell culture device 20 (S7), whereby the storage of the pipette chip 1 is completed.

本実施形態の三重滅菌包装物を使用すれば、滅菌済みのピペット用チップ、ディッシュ、遠心管、サンプル管等の消耗品を、その付随物であるスタンドやラベルなどを含めてクリーン度の高い自動細胞培養装置20内に搬入することができる。また、本実施形態の滅菌物の入庫方法によれば、上記三重滅菌包装物を使用して、クリーン度の低い外部から順次クリーン度の高いエリアに高いクリーン度を維持したまま滅菌物を搬入することが可能となる。   If the triple sterilization package of this embodiment is used, sterilized pipette tips, dishes, centrifuge tubes, sample tubes, and other consumables are automatically cleaned with a high degree of cleanliness, including their accompanying stands and labels. It can be carried into the cell culture device 20. In addition, according to the method for storing a sterilized product according to the present embodiment, the sterilized product is transferred from the outside with a low cleanness to an area with a high cleanliness level while maintaining a high cleanness level. It becomes possible.

なお、上記では滅菌物を滅菌袋を用いて三重に包装した三重滅菌包装物について説明したが、本発明はこれに限定されるものではなく、要求されるクリーン度に応じて何重に包装するかを決定することができる。   In addition, although the above demonstrated the triple sterilization package which packaged the sterilization thing three times using the sterilization bag, this invention is not limited to this, It wraps several layers according to the required cleanliness Can be determined.

本発明の多重滅菌包装物及び滅菌物の入庫方法を使用すれば、クリーン度を殆ど低下させることなく滅菌物の入庫を行うことが可能なので、細胞培養装置、無菌培養装置等の分野で利用可能である。   Use of the multiple sterilized packaging and sterilized product storage method of the present invention enables storage of sterilized products with almost no reduction in cleanliness, and can be used in the field of cell culture devices, aseptic culture devices, etc. It is.

本発明の多重滅菌包装物によって包装される消耗品を表す模式図である。It is a schematic diagram showing the consumable goods packaged with the multiple sterilization package of this invention. 図1の消耗品を多重包装した多重滅菌包装物を表す模式図である。It is a schematic diagram showing the multiple sterilization package which carried out the multiple packaging of the consumable goods of FIG. 本発明の多重滅菌包装物が搬入され、本発明の滅菌物の入庫方法が実施される自動細胞培養装置の模式図である。It is a schematic diagram of the automatic cell culture apparatus in which the multiple sterilization package of this invention is carried in and the storage method of the sterilization thing of this invention is implemented. 図3の入庫部の詳細を示す斜視図である。It is a perspective view which shows the detail of the warehousing part of FIG. 外部からクリーンルーム及び入庫部を介して自動細胞培養装置内に三重滅菌包装物内の滅菌物を入庫する本発明の滅菌物の入庫方法の一実施形態を示す模式図である。It is a schematic diagram which shows one Embodiment of the storage method of the sterilized material of this invention which stores the sterilized material in a triple sterilization package in an automatic cell culture apparatus from the outside via a clean room and a storage part. 図5の入庫方法を示すフローチャートである。It is a flowchart which shows the warehousing method of FIG.

符号の説明Explanation of symbols

1 ピペット用チップ
2 ディッシュ
3 遠心管
4 サンプル管
1a チップ用スタンド
2a ディッシュ用スタンド
3a 遠心管用スタンド
4a サンプル管用スタンド
2b,3b,4b ラベル
5a,5b,5c 滅菌袋
6,7,8,9 三重滅菌包装物
20 自動細胞培養装置
10 機器設置部
11 操作ロボット
12 インキュベータ部
13 操作部
14 入庫部
15 グローブボックス
16 連通部
17 扉
18a,18b 操作穴
19 ボックス本体
23 台車
24 レール
25 パスボックス
6’ 二重滅菌包装物
6” 一重滅菌包装物
1 Pipette tip
2 dishes
3 Centrifuge tube
4 Sample tube
1a Stand for chip
2a Dish stand
3a Centrifuge tube stand
4a Sample tube stand 2b, 3b, 4b Label 5a, 5b, 5c Sterilization bag 6, 7, 8, 9 Triple sterilization package
20 Automatic cell culture equipment
10 Equipment Installation Department
11 Operation robot
12 Incubator
13 Operation part
14 Receipt Department
15 Glove box
16 communication part
17 Door
18a, 18b Operation hole
19 Box body
23 trolleys
24 rails
25 pass box
6 'Double sterile packaging
6 "single sterilized packaging

Claims (5)

滅菌物を滅菌袋を用いて多重包装した多重滅菌包装物であって、最外部の滅菌袋の外側を除き、各滅菌袋の内側及び外側が滅菌状態であることを特徴とする多重滅菌包装物。   A multiple sterilization package in which a sterilization product is multiple-wrapped using a sterilization bag, and the inside and outside of each sterilization bag are in a sterilized state except for the outside of the outermost sterilization bag. . 前記滅菌物が、滅菌済みのピペット用チップ、ディッシュ、遠心管及びサンプル管の何れかである請求項1記載の多重滅菌包装物。   The multiple sterilized package according to claim 1, wherein the sterilized product is one of a sterilized pipette tip, a dish, a centrifuge tube, and a sample tube. クリーン度の低いエリアからクリーン度の高いエリアに滅菌物を移動させて入庫を行う請求項1又は2に記載の多重滅菌包装物を用いた滅菌物の入庫方法であって、前記多重滅菌包装物を収容している所定のクリーン度のエリアにおいて、前記多重滅菌包装物の最外部の滅菌袋から中身を取り出して、クリーン度の次に高いエリアに搬入することを特徴とする滅菌物の入庫方法。   The sterilized product storage method using the multiple sterilized package according to claim 1 or 2, wherein the sterilized product is moved from an area with a low cleanness to an area with a high cleanness, and the sterilized product is stored. Sterilized article storage method, in which the contents are taken out from the outermost sterilization bag of the multiple sterilized package in an area of a predetermined cleanliness containing the sterilized product and are carried into an area having the next highest cleanliness . 前記所定のクリーン度のエリアにおいて、クリーン度がクリーン度の次に高いエリアのクリーン度と同じになった後に、前記多重滅菌包装物の最外部の滅菌袋から中身を取り出して前記クリーン度の次に高いエリアに搬入することを特徴とする請求項3に記載の滅菌物の入庫方法。   In the area of the predetermined cleanness level, after the cleanliness level becomes the same as the cleanliness level of the next higher cleanness area, the contents are taken out from the outermost sterilization bag of the multiple sterilization package and the next cleanliness level is obtained. 4. The sterilized product storage method according to claim 3, wherein the sterilized product is stored in a high area. 請求項3又は4に記載の滅菌物の入庫方法を複数回繰り返すことを特徴とする滅菌物の入庫方法。   5. A method for storing a sterilized product, wherein the method for storing a sterilized product according to claim 3 or 4 is repeated a plurality of times.
JP2007078776A 2007-03-26 2007-03-26 Multiplex sterilized package and method for warehousing sterilized object using the same Pending JP2008239168A (en)

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