JP2008145109A - Evaluation device of horny layer cell - Google Patents
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- JP2008145109A JP2008145109A JP2006328955A JP2006328955A JP2008145109A JP 2008145109 A JP2008145109 A JP 2008145109A JP 2006328955 A JP2006328955 A JP 2006328955A JP 2006328955 A JP2006328955 A JP 2006328955A JP 2008145109 A JP2008145109 A JP 2008145109A
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Abstract
Description
本発明は、皮膚表面の角層細胞の評価装置に関する。より具体的には、非染色の角層細胞の鑑別において、角層細胞を採取した支持体を簡易に固定し、且つ、容易に移動できることを特徴とする、角層細胞の評価装置に関する。 The present invention relates to an evaluation apparatus for horny layer cells on the skin surface. More specifically, the present invention relates to a stratum corneum cell evaluation apparatus characterized in that, in differentiation of unstained stratum corneum cells, the support from which the stratum corneum cells are collected can be easily fixed and easily moved.
化粧料を使用するにあたり、重要な事項は適切な化粧料を選択することである。この様な適切な化粧料の選択を行う必要条件としては、皮膚の状態或いは特性を正しく鑑別することが挙げられる。このための皮膚の状態或いは特性を正しく鑑別するための技術としては、顔の頬等の部位より粘着テープ等を用いて、ストリッピングにより角層細胞を採取し、ゲンチアナバイオレット等の染色剤を用いて角層細胞を染色し（例えば、特許文献１、特許文献２参照）、角層細胞の形状を明確にし、角層細胞の面積、体積、厚さ、配列規則性、或いは角層細胞の剥がれ具合を測定して、その値を指標値にしてバリアー機能、肌質、肌性等の肌状態を鑑別する技術が既に確立されている（特許文献３、特許文献４、特許文献５参照）。即ち、面積や体積が小さいほど、或いは配列規則性が悪いほど角層細胞が充分成熟しないうちに最外部に上がってきてしまっており、皮膚バリア機能は低く、敏感肌であり、肌の状態が悪いと鑑別する。この様な鑑別の基礎は角層細胞の形状であり、そのため先に述べた染色剤による染色工程が不可欠であったが、この工程には所要時間、作業スペース及び環境問題等の大きな課題があった。 In using cosmetics, an important matter is to select appropriate cosmetics. A necessary condition for selecting such an appropriate cosmetic is to correctly distinguish the state or characteristics of the skin. For this purpose, as a technique for correctly distinguishing the state or characteristics of the skin, the stratum corneum cells are collected by stripping from an area such as the cheek of the face, and a stain such as gentian violet is used. The stratum corneum cells are stained (see, for example, Patent Document 1 and Patent Document 2), the shape of the stratum corneum cells is clarified, and the area, volume, thickness, regularity of the stratum corneum cells, or peeling of the stratum corneum cells The technique which measures a condition and discriminate | determines skin conditions, such as a barrier function, skin quality, and skin property, using the value as an index value is already established (refer patent document 3, patent document 4, patent document 5). In other words, the smaller the area and volume, or the poorer the regularity of the arrangement, the horny layer cells have come to the outermost part before they mature sufficiently, the skin barrier function is low, the skin is sensitive, and the skin condition is Differentiate from bad. The basis of such differentiation is the shape of the stratum corneum cells, and therefore, the dyeing process with the above-mentioned staining agent was indispensable. However, this process has major problems such as required time, work space and environmental problems. It was.
これらの課題を克服すべく、非染色的な評価技術として、皮膚表面形態の観察などに用いられていた、ビデオマイクロスコープを応用した観察装置（特許文献６、特許文献７参照）が開示されている。また、これらに対して画像処理技術を用い、人による評価に伴う作業負担の軽減と精度及び速度の向上を図ろうとする技術開発も試みられている。例えば、透過光量に基づいて角層細胞剥離量を判定する方法（特許文献８参照）、透過画像に対して画像処理を行って角層細胞剥離量と角層細胞剥離均一性の両方を解析する方法（特許文献９参照）、自己相関マスクパターンを用いた学習型画像計測装置による角層細胞面積やその剥がれ具合を分析する方法（特許文献１０参照）、多変量解析を利用した角層細胞面積と剥がれ具合の自動評価法（特許文献１１参照）、ニューラルネットワークを利用した角層細胞等の評価装置（特許文献１２参照）なども開示されている。 In order to overcome these problems, as a non-staining evaluation technique, an observation apparatus (see Patent Document 6 and Patent Document 7) using a video microscope, which has been used for observation of skin surface morphology, etc. is disclosed. Yes. In addition, an attempt has been made to develop a technique for reducing the work burden associated with human evaluation and improving accuracy and speed by using an image processing technique. For example, a method for determining the amount of stratum corneum detachment based on the amount of transmitted light (see Patent Document 8), and performing image processing on a transmitted image to analyze both the amount of stratum corneum detachment and the uniformity of stratum corneum detachment A method (see Patent Document 9), a method of analyzing the stratum corneum cell area and the degree of detachment by a learning image measuring device using an autocorrelation mask pattern (see Patent Document 10), and a stratum corneum cell area using multivariate analysis In addition, an automatic evaluation method for peeling degree (see Patent Document 11), an evaluation device for horny layer cells using a neural network (see Patent Document 12), and the like are also disclosed.
かような状況下において、角層細胞の評価に際して、現実の肌状態が変化する部位と変化しない部位がモザイク状に混在することから、実際の肌質と角層細胞の評価とを高精度に一致させるには、５０〜１００個程度の角層細胞を測定の対象とすることが極めて重要な要件である（特許文献１３参照）。このため、角層細胞を採取した支持体を簡易に固定し、且つ移動させて複数回数の観察を行うことが極めて重要な要素である。特に、非染色標本を用いた場合には、評価可能な細胞数の割合が非常に少なくなるため、この技術はより重要になってくる。更に、標本における細胞の部分と粘着剤の部分のコントラストを明確にすることも重要となってくる。しかしながら、実際には角層細胞を採取した支持体を単に台座に置いたり、粘着テープなどで固定する等の対応が為されており、かような技術については未だに得られていないのが現状といえる。コントラストについても何ら検討が加えられていないと言える。 Under such circumstances, when evaluating stratum corneum cells, the actual skin condition and stratum corneum cell evaluation can be performed with high accuracy because the areas where the actual skin condition changes and the areas where it does not change are mixed in a mosaic pattern. In order to make it coincide, it is a very important requirement to measure about 50 to 100 stratum corneum cells (see Patent Document 13). For this reason, it is an extremely important element that the support from which the stratum corneum cells are collected is simply fixed and moved and observed multiple times. In particular, when a non-stained specimen is used, this technique becomes more important because the ratio of the number of cells that can be evaluated becomes very small. It is also important to clarify the contrast between the cell portion and the adhesive portion in the specimen. However, in reality, the support from which the stratum corneum cells are collected is simply placed on a pedestal or fixed with an adhesive tape, etc., and such technology has not yet been obtained. I can say that. It can be said that no consideration has been given to contrast.
本発明はこのような状況下で為されたものであり、非染色角層細胞の評価に於いて、その精度が染色細胞と同程度に維持できる、角層細胞の評価装置を提供することを課題とする。 The present invention has been made under such circumstances, and provides an evaluation device for stratum corneum cells, which can maintain the same accuracy as stained cells in the evaluation of unstained stratum corneum cells. Let it be an issue.
この様な実状に鑑みて、本発明者らは、非染色角層細胞の評価に於いて、その精度が染色細胞と同程度に維持できる、角層細胞の評価装置を求めて、鋭意研究努力を重ねた結果、皮膚表面の角層細胞の評価装置であって、情報処理部、表示部、拡大ビデオカメラ部及び標本における焦点を変えることなく平面移動可能な、標本固定部からなり、該標本固定部は、拡大ビデオカメラ部に接続されるべき構造を有し、皮膚より採取した角層細胞を有する支持体部と、該支持体部を保持し且つ容易にスライドさせるためのスライド機構部とを有し、標本固定部における、ビデオカメラに対する底面が黒色に色調を調整されている装置が、多くの視野を効率よく確保でき、且つ、角層細胞と背景の粘着剤との間に優れたコントラストを付与する作用により、この様な精度を具現化出来ることを見いだし、発明を完成させるに至った。即ち、本発明は以下に示すとおりである。
（１）皮膚表面の角層細胞の評価装置であって、情報処理部、表示部、拡大ビデオカメラ部及び標本における焦点を変えることなく平面移動可能な、標本固定部からなり、該標本固定部は、拡大ビデオカメラ部に接続されるべき構造を有し、皮膚より採取した角層細胞を有する支持体部と、該支持体部を保持し且つ容易にスライドさせるためのスライド機構部とを有し、標本固定部における、ビデオカメラに対する底面が黒色に色調を調整されていることを特徴とする、角層細胞の評価装置。
（２）前記角層細胞の評価装置の標本固定部に設置されるべき標本は、片面に粘着部を有する、透明支持体の粘着部に皮膚を圧着させ、角層細胞を剥離したものであって、粘着部を有しない面をビデオカメラに向けて挿入されたものであることを特徴とする、（１）に記載の角層細胞の評価装置。
（３）前記スライド機構部に、前記支持体を挿入するための溝構造を有することを特徴とする、（１）又は（２）に記載の角層細胞の評価装置。
（４）前記スライド機構部に、前記支持体を保持する支持体保持器を有することを特徴とする、（１）〜（３）何れか１項に記載の角層細胞の評価装置
（５）前記支持体保持器が、プランジャ又はスプリングボールプランジャであることを特徴とする、（１）〜（４）に記載の角層細胞の評価装置。
（６）前記プランジャ又はスプリングボールプランジャを２個以上配置することを特徴とする、（１）〜（５）に記載の角層細胞の評価装置。
In view of such a situation, the present inventors have sought for an evaluation device for stratum corneum cells that can maintain the accuracy of unstained stratum corneum cells at the same level as stained cells. Is a device for evaluating horny layer cells on the skin surface, comprising an information processing section, a display section, an enlarged video camera section, and a specimen fixing section that can move in a plane without changing the focal point of the specimen. The fixing unit has a structure to be connected to the enlarged video camera unit, and includes a support unit having stratum corneum cells collected from the skin, and a slide mechanism unit for holding and easily sliding the support unit. The device with the bottom of the video camera in the specimen fixing part, which is adjusted to a black color tone, can efficiently secure many fields of view and is excellent between the stratum corneum cells and the background adhesive. For the effect of adding contrast Ri, found that can be embodied such a precision, has led to the completion of the invention. That is, the present invention is as follows.
(1) An apparatus for evaluating stratum corneum cells on the skin surface, comprising an information processing unit, a display unit, an enlarged video camera unit, and a sample fixing unit capable of moving in a plane without changing the focal point of the sample, the sample fixing unit Has a structure to be connected to the magnified video camera unit, and has a support part having stratum corneum cells collected from the skin, and a slide mechanism part for holding and easily sliding the support part. In addition, the stratum corneum cell evaluation apparatus is characterized in that the bottom surface of the specimen fixing unit with respect to the video camera is adjusted to a black color tone.
(2) The specimen to be placed in the specimen fixing part of the stratum corneum cell evaluation apparatus has a sticking part on one side, and the skin is pressure-bonded to the sticking part of the transparent support, and the stratum corneum cells are peeled off. The device for evaluating horny layer cells according to (1), wherein the surface having no adhesive portion is inserted toward the video camera.
(3) The stratum corneum cell evaluation apparatus according to (1) or (2), wherein the slide mechanism section has a groove structure for inserting the support.
(4) The evaluation device (5) for stratum corneum cells according to any one of (1) to (3), wherein the slide mechanism unit has a support holder for holding the support. The stratum corneum cell evaluation apparatus according to any one of (1) to (4), wherein the support holder is a plunger or a spring ball plunger.
(6) The stratum corneum cell evaluation apparatus according to any one of (1) to (5), wherein two or more of the plungers or spring ball plungers are arranged.
本発明によれば、非染色角層細胞の評価に於いて、その精度が染色細胞と同程度に維持できる、角層細胞の評価装置を提供することができる。 ADVANTAGE OF THE INVENTION According to this invention, in the evaluation of a non-stained stratum corneum cell, the evaluation apparatus of a stratum corneum cell which can maintain the precision comparable to a dyed cell can be provided.
本発明の角層細胞の評価装置は、複数回数の角層細胞の評価を容易、高速且つ高精度に行うことを目的とし、皮膚表面の角層細胞の評価装置であって、情報処理部、表示部、拡大ビデオカメラ部及び標本における焦点を変えることなく平面移動可能な、標本固定部からなり、該標本固定部は、拡大ビデオカメラ部に接続されるべき構造を有し、角層細胞を採取した支持体を簡易に固定し且つ容易に移動させる機構を備えた、角層細胞の評価のための評価装置に関する。前記拡大ビデオカメラシステムは、拡大画像情報を取り込むビデオカメラ部、該ビデオカメラ部で取り込まれた拡大画像情報を演算処理するパーソナルコンピュータなどからなる情報処理部、該情報処理結果を表示する、ＣＲＴ等の表示部を有し、具体的には、特許文献８、特許文献９に開示された拡大ビデオシステムを用いることができる。 The evaluation device for horny layer cells of the present invention is an evaluation device for horny layer cells on the skin surface for the purpose of easy, high-speed and high-precision evaluation of a plurality of times of horny layer cells, comprising an information processing unit, A display unit, an enlarged video camera unit, and a specimen fixing unit that can be moved in a plane without changing the focal point in the specimen. The specimen fixing unit has a structure to be connected to the enlarged video camera unit, The present invention relates to an evaluation apparatus for evaluating stratum corneum cells, which is provided with a mechanism for easily fixing a collected support and moving it easily. The enlarged video camera system includes a video camera unit that captures enlarged image information, an information processing unit that includes a personal computer that performs arithmetic processing on the enlarged image information captured by the video camera unit, a CRT that displays the information processing result, and the like. Specifically, the enlarged video system disclosed in Patent Literature 8 and Patent Literature 9 can be used.
又、観察、評価されるべき角層細胞の標本としては、皮膚より採取して後、なるべく処理を加えない状態で観察、評価に供されることが、標本の採取時の形態の維持性と、評価結果の利用可能性の面で好ましい。即ち、採取より評価結果がアウトプットされるまでの時間が長すぎた場合には、評価結果がスキンケアの為の化粧料選択などの応用用途に供しにくくなることが存する為である。この様な視点から、片面に粘着部を有する、透明支持体の粘着部に皮膚を圧着させ、角層細胞を剥離したものを用いることが好ましい。この様な形態の標本は、予めガラスやプラスチックなどの透明支持体の片面に粘着剤を塗工した採取用具を用意しておき、観察時に、採取用具を皮膚に圧着させ、剥離し、そのまま標本として観察することにより、リアルタイムの処理がなしうるからである。従って、本発明の装置はこの様な標本を観察、評価対象にしたものである。 In addition, as a sample of stratum corneum cells to be observed and evaluated, after being collected from the skin, it may be subjected to observation and evaluation in a state where treatment is not performed as much as possible. This is preferable in terms of availability of evaluation results. That is, if the time from the collection to the output of the evaluation result is too long, the evaluation result may be difficult to be used for application such as selection of cosmetics for skin care. From such a point of view, it is preferable to use one having an adhesive part on one side and having the skin pressure-bonded to the adhesive part of the transparent support and exfoliating the stratum corneum cells. For specimens of this type, prepare a sampling tool that has been coated with adhesive on one side of a transparent support such as glass or plastic in advance. This is because real-time processing can be performed by observing as follows. Therefore, the apparatus of the present invention is for observing and evaluating such a specimen.
本発明の評価装置を用いて行う角層細胞の評価項目とは、例えば、角層の剥がれ具合、角層細胞の配列規則性、或いは角層細胞面積等である。前記角層の剥がれ具合とは、角層細胞がまとまった剥離が頻繁に観察されるか、何層も重なっているのか、若しくは重ならないで１層であるのか、であり、前記角層細胞の配列規則性とは、角層細胞の大きさが揃っておりそのバラツキが少ないことと、細胞個々の形状が形の崩れていない六角形構造をしっかり維持し角層細胞同士の配列が規則的であることとを、どの程度満たしているかということで定義されており、また、角層細胞面積とは、個々の角層細胞面積、その平均や分散である。角層のバリアー機能、肌性や肌質等の重要な肌特性を知るためには、これらの評価項目を評価することが極めて重要な要素である。 The evaluation items of the stratum corneum performed using the evaluation apparatus of the present invention are, for example, the degree of peeling of the stratum corneum, the arrangement regularity of the stratum corneum, or the stratum corneum cell area. The degree of exfoliation of the stratum corneum is whether the exfoliation of the stratum corneum cells is frequently observed, whether there are multiple layers, or one layer without overlapping, The regularity of the stratum corneum is that the size of the stratum corneum cells is uniform and there is little variation. It is defined by the degree to which one is satisfied, and the stratum corneum cell area is an individual stratum corneum cell area, its average or variance. In order to know important skin characteristics such as the barrier function of the stratum corneum, skin properties and skin quality, it is extremely important to evaluate these evaluation items.
以下に、図面を参照して、本発明を実施するための最良の形態を、実施例に基づいて例示的に詳しく説明する。但し、この実施例に記載されている構成部品の寸法、材質、形状、その相対配置等は、特に特定な記載がない限りは、この発明の範囲をそれらのみに限定する趣旨のものではない。図１は、本発明にかかる一実施例の角層細胞の観察に用いる評価装置の全体構成を示す図である。図１の装置は、角層細胞を採取した支持体を簡易に固定し、且つ角層細胞標本の焦点を変えることなく平面移動可能な機能を有する標本固定部（１０）であって該固定部の標本に面した面は黒色に塗工されており、該標本固定部と接続された拡大ビデオカメラ部（２０）と、該拡大ビデオカメラ部よりの拡大画像情報を演算処理する情報処理部・パーソナルコンピュータ（３０）と、該拡大画像や該画像の演算処理結果を表示する表示部・ＣＲＴ（４０）、とにより構成されている。 The best mode for carrying out the present invention will be exemplarily described in detail below based on examples with reference to the drawings. However, the dimensions, materials, shapes, relative arrangements, and the like of the components described in this embodiment are not intended to limit the scope of the present invention only to those unless otherwise specified. FIG. 1 is a diagram showing an overall configuration of an evaluation apparatus used for observation of stratum corneum cells according to one embodiment of the present invention. The apparatus of FIG. 1 is a specimen fixing section (10) having a function of easily fixing a support from which horny layer cells have been collected and capable of moving in a plane without changing the focal point of the horny layer cell specimen. The surface facing the specimen is coated in black, an enlarged video camera section (20) connected to the specimen fixing section, and an information processing section for processing the enlarged image information from the enlarged video camera section A personal computer (30) and a display unit / CRT (40) for displaying the enlarged image and the calculation processing result of the image are configured.
図２に、標本固定部（１０）の立面図及び平面図を、また図４にその斜視図を示すが、これらを用いてさらに詳細に説明する。拡大ビデオカメラ部（２０）のレンズを保持したレンズドーム（２１）は、３つのパーツからなる本体部Ａ（１１）、本体部Ｂ（１２）及びスライド機構部（１３）に装着されている。本体Ａには、Ｏリング（１５）及び締め付けボルト（１４）が装着され、レンズドームとの固定をより強固にしている。則ち、レンズドームと本体Ａとは、共に樹脂製であるために、ゴム製のＯリングを介してボルトで締め付けることでより強固な締め付けが可能であり、また、ピント調整やレンズ交換の操作性も容易となる。レンズを装着する場合は、レンズドームが本体Ｂに当たる位置まで挿入するが、これはレンズの装着位置（レンズドームの先端）を常に所定のポイントに位置決めできる為である。また、後述する支持体部（１７）を所定のポイント（レンズドーム先端からの距離）に位置決めできる機能も有している。 FIG. 2 shows an elevational view and a plan view of the specimen fixing portion (10), and FIG. 4 shows a perspective view thereof, which will be described in further detail. The lens dome (21) holding the lens of the magnifying video camera unit (20) is mounted on a main body part A (11), a main body part B (12) and a slide mechanism part (13) comprising three parts. The main body A is provided with an O-ring (15) and a tightening bolt (14) to further secure the lens dome. In other words, since the lens dome and the main body A are both made of resin, they can be tightened with bolts via a rubber O-ring, and the focus adjustment and lens replacement operations can be performed. Also becomes easier. When the lens is mounted, the lens dome is inserted to a position where it comes into contact with the main body B. This is because the lens mounting position (tip of the lens dome) can always be positioned at a predetermined point. Moreover, it has the function which can position the support body part (17) mentioned later to the predetermined | prescribed point (distance from a lens dome front-end | tip).
図２において、スライド機構部（１３）には、皮膚より採取した角層細胞を有する支持体部（１７）を挿入するための溝構造を備えている。かような溝構造によって、支持体部に付着した、皮膚より採取した角層細胞の観察を行う際に、容易に移動させて複数回の観察を行い、評価データを得ることができる。又、スライド機構部の支持体部に面した面は黒く塗工されている。かかる塗工により、非染色の標本でも角層細胞と背景となっている粘着部との間のコントラストが確保されており、これにより視野を多くサーベイすることにより、非染色の条件でも精度の高い角層細胞の面積を求めることが出来る。スライド機構部（１３）の基盤となる部位の図（図面代用写真）を図５に示す。 In FIG. 2, the slide mechanism part (13) is provided with a groove structure for inserting a support part (17) having stratum corneum cells collected from the skin. With such a groove structure, when the stratum corneum cells collected from the skin attached to the support body are observed, it is easily moved and observed a plurality of times to obtain evaluation data. The surface of the slide mechanism that faces the support is painted black. By this coating, the contrast between the stratum corneum cells and the background adhesive part is ensured even in the unstained specimen, and by this, by surveying a large field of view, it is highly accurate even in the unstained condition. The area of stratum corneum cells can be determined. FIG. 5 shows a diagram (photograph substituted for a drawing) of a portion serving as a base of the slide mechanism portion (13).
図３に、標本固定部の構成要素を示す。標本固定部のスライド機構部（１３）には、支持体（１７）を保持する支持体保持器（１９）を有することが好ましい。該支持体保持器は、採取した角層細胞を有する支持体を一定の力で保持し、且つ支持体の位置を微妙に移動させることが可能な機能を有する機構である。支持体部を保持するための支持体保持器に要する力は、１．３Ｎ〜６．４Ｎ程度が望ましい。かような力を発揮できる機能を持つものとしては、例えば、板バネ、皿バネ、コイルバネ、スプリング、プランジャ又はスプリングボールプランジャ等を例示でき、材質はステンレス、鉄、銅、アルミなど特に限定することなく利用することができる。 FIG. 3 shows components of the specimen fixing unit. The slide mechanism part (13) of the specimen fixing part preferably has a support holder (19) that holds the support (17). The support holder is a mechanism having a function capable of holding a support having collected stratum corneum cells with a constant force and finely moving the position of the support. The force required for the support holder for holding the support section is preferably about 1.3N to 6.4N. Examples of such a function capable of exerting such a force include a leaf spring, a disc spring, a coil spring, a spring, a plunger, or a spring ball plunger, and the material is particularly limited to stainless steel, iron, copper, aluminum, and the like. It can be used without.
前記プランジャ（１８）又はスプリングボールプランジャ（１８）は、図２に示されるような形状を有し、各種形状・タイプのプランジャが産業用機械部品として市販（例えば、株式会社ナベヤ、株式会社タカイコーポレーション）されており、特に限定することなく用いることができるが、円筒形のスプリング部の先端にボール（球状突起）を内蔵したタイプのスプリングボールプランジャが特に好ましい。該スプリングボールプランジャの材質は、ボール部及びスプリング部とも、ステンレス、真鍮或いはプラスティック等であるが、いずれであっても精度及び耐久性とも十分であり特に限定されず、ボールやスプリング等のサイズも、特に限定を受けず、支持体との相対的サイズによって定まる。また、その取り付け位置は、例えば、図３のように、採取した角層細胞を挟むような対称的位置が好ましく、スライド機構部に２個以上を装着して、角層細胞を有する支持体部の樹脂部分に接触するようにすることが望ましい。則ち、スプリングボールプランジャはそのスプリングによる圧をボール先端を介し、支持体を本体Ｂに押し付けることで、支持体とレンズドームの先端の距離を一定且つ水平に保つ機能を有している。角層細胞の評価項目において、多数の角層細胞の視野を観察するために、３〜１０回の支持体の位置を微妙に移動させるが、その際に、かようなスプリングボールプランジャによる本体Ｂへの適切な圧迫によって、支持体の位置の微妙な調整が容易に行え、且つ水平維持機能によりピントも殆どずれることがないという、極めて有利な効果を発揮している。これにより多くの視野をピントずれを起こさずにサーべーできる。 The plunger (18) or the spring ball plunger (18) has a shape as shown in FIG. 2, and various shapes and types of plungers are commercially available as industrial machine parts (for example, Nabeya Co., Ltd., Takai Corporation). However, a spring ball plunger of a type in which a ball (spherical protrusion) is built in the tip of a cylindrical spring portion is particularly preferable. The material of the spring ball plunger is stainless steel, brass, plastic, or the like for both the ball part and the spring part. There is no particular limitation, and it is determined by the relative size to the support. Further, the attachment position is preferably a symmetrical position, for example, as shown in FIG. 3 so as to sandwich the collected horny layer cells. Two or more slide mechanism parts are attached to the support part having the horny layer cells. It is desirable to be in contact with the resin part of. In other words, the spring ball plunger has a function of keeping the distance between the support body and the tip of the lens dome constant and horizontal by pressing the support body against the main body B through the pressure of the spring through the tip of the ball. In the evaluation item of the stratum corneum, in order to observe the field of view of a large number of stratum corneum cells, the position of the support is slightly moved 3 to 10 times. With appropriate compression, the position of the support can be easily finely adjusted, and a very advantageous effect is achieved in that the focus is hardly shifted by the horizontal maintenance function. As a result, many fields of view can be surveyed without causing out of focus.
かくして、皮膚の諸観察に汎用性のある、拡大ビデオシステムを利用した、本発明の角層細胞の評価装置を用いることにより、角層細胞を採取した支持体を、非染色の条件で、簡易に固定し且つ容易に移動させて、複数回数の角層細胞の評価を容易、高速且つ高精度に行うことが可能となる。 Thus, by using the corneal cell evaluation apparatus of the present invention using an enlarged video system that is versatile for various skin observations, the support from which the horny layer cells are collected can be easily obtained under non-staining conditions. It is possible to easily evaluate the stratum corneum cells a plurality of times at high speed and with high accuracy.
このように、角層細胞を有する支持体部を簡易に固定し且つ容易に移動させて、複数回数の角層細胞の評価を、容易、高速且つ高精度に行うことが可能となり、黒色の背景効果により、角層細胞と粘着剤部分のコントラストも高まり、非染色の条件でも角層細胞の面積を精度高く短時間に計測できる効果を奏する。則ち、かような機構を有していない場合、例えば、角層細胞を採取した支持体を単に台座に置いたり、粘着テープなどで固定した場合では、現実の肌状態が変化する部位と変化しない部位がモザイク状に混在することから、実際の肌質と角層細胞の評価とを高精度に一致させるために、境界明瞭な５０〜１００個程度の角層細胞を測定の対象とする必要があり、角層細胞を有する支持体を幾度も移動させ、その都度角層細胞のピント調整を行うことが必須である。しかし、本発明によって、そのような操作が不要となり、本発明の対象となる角層細胞面積、角層の剥がれ具合、或いは角層細胞の配列規則性等を容易、高速且つ高精度に行うことが可能となる。又、背景が白い場合には面積を境界が非鮮明になり、角層細胞の面積を正確に測定することは容易ではない。 In this way, it is possible to easily fix and easily move the support portion having the stratum corneum cells, and to evaluate the stratum corneum cells a plurality of times easily, at high speed and with high accuracy. As a result, the contrast between the stratum corneum cells and the pressure-sensitive adhesive portion is increased, and the area of the stratum corneum cells can be measured with high accuracy and in a short time even under non-staining conditions. In other words, when such a mechanism is not provided, for example, when the support from which the stratum corneum cells are collected is simply placed on a pedestal or fixed with an adhesive tape, the actual skin condition changes and changes. In order to match the actual skin quality and the evaluation of the stratum corneum with high accuracy, it is necessary to measure about 50 to 100 stratum corneum cells with clear boundaries. It is essential to move the support having stratum corneum cells several times and to adjust the focus of the stratum corneum cells each time. However, according to the present invention, such an operation becomes unnecessary, and the stratum corneum cell area, the stratum corneum peeling, or the regularity of the stratum corneum cells, which is the object of the present invention, can be easily, rapidly and accurately performed. Is possible. In addition, when the background is white, the boundary of the area becomes unclear, and it is not easy to accurately measure the area of the stratum corneum cells.
以下に、図１、２に示す本発明の角層細胞の評価装置を用いて、評価した試験例を挙げて、さらに詳細に説明を加えるが、本発明がこれら実施例、試験例にのみ限定されないことは言うまでもない。 The test examples evaluated using the evaluation apparatus for horny layer cells of the present invention shown in FIGS. 1 and 2 will be described below in further detail, but the present invention is limited only to these examples and test examples. It goes without saying that it is not done.
＜試験例１＞
本発明の対象である、角層細胞の面積について、本発明の、標本の背景が黒になるように黒く塗工した、スライド機構部を有する角層細胞の評価装置を用いた場合（本発明１）と、更にスプリングボールプランジャを備えた、標本の背景が黒になるように黒く塗工した、スライド機構部を有する角層細胞の評価装置を用いた場合（本発明２）と、単に角層細胞を採取した支持体を台座に置いて移動させた場合（従来法）における、容易性、高速性を所要時間として比較した。（対照１）は、全ての計測の終了後にこの標本をゲンチアナバイオレットで染色し、本発明２の装置で測定したものとした。（対照２）は、この染色標本を本発明２の装置の標本に面した面を白く塗工したもので計測したものとした。則ち、１８〜６５才までの女性被験者の頬部より、定法によりテープストリッピング法で採取した支持体上の角層細胞を非染色で、粘着剤の塗工していない側をカメラに向けて設置し、これを観察し、角層細胞の面積測定に適した角層細胞標本５０個を選択し、これを画像として取込み、５０個の細胞の平均面積と標準偏差を求めた。この結果を表１に示す。これより、本発明の装置においては、非染色の条件でも染色条件と同様の精度を有することがわかる。
<Test Example 1>
When the horny layer cell evaluation apparatus having the slide mechanism portion coated with black so that the background of the specimen is black is used for the area of the horny layer cell which is the object of the present invention (the present invention) 1) and a case of using an evaluation device for stratum corneum cells having a slide mechanism part, which is further provided with a spring ball plunger and is coated black so that the background of the specimen is black (invention 2), When the support from which the layer cells were collected was moved on a pedestal (conventional method), the ease and high speed were compared as the required time. In (Control 1), this sample was stained with gentian violet after completion of all measurements, and measured with the apparatus of the present invention 2. (Control 2) was obtained by measuring the stained specimen by coating the surface facing the specimen of the apparatus of the present invention 2 in white. In other words, from the cheeks of female subjects aged 18 to 65, the stratum corneum cells on the support collected by the tape stripping method are not stained and the side not coated with the adhesive is pointed at the camera. The sample was placed and observed, and 50 horny layer cell specimens suitable for measuring the area of the horny layer cells were selected. The results are shown in Table 1. From this, it can be seen that the apparatus of the present invention has the same accuracy as the staining condition even in the non-staining condition.
＜試験例２＞
非染色条件での角層細胞像への、背景となる支持体の基盤の塗工色の影響を調べた。実施例１の本発明２で得られた画像を図６に示す。対照２の装置で非染色標本を観察して得られた画像を図７に示す。参考としての対照２の画像を図８に示す。これより背景色を黒にすることにより角層細胞の辺縁が明確になっていることがわかる。
<Test Example 2>
The effect of the coating color of the base material of the support as a background on the stratum corneum cell image under non-staining conditions was examined. The image obtained by the present invention 2 of Example 1 is shown in FIG. FIG. 7 shows an image obtained by observing the unstained specimen with the control 2 apparatus. An image of Control 2 as a reference is shown in FIG. From this, it can be seen that the edge of the stratum corneum cells is clear by setting the background color to black.
本発明は化粧料の選択などに有用な情報となる角層細胞の計測に応用できる。 The present invention can be applied to the measurement of horny layer cells, which is useful information for selecting cosmetics.
１０ 標本固定部
２０ 拡大ビデオカメラ部
３０ 情報処理部（パーソナルコンピュータ）
４０ 表示部（ＣＲＴ）
１１ 本体部Ａ
１２ 本体部Ｂ
１３ スライド機構部
１４ 締め付けボルト
１５ Ｏリング
１７ 支持体
１８ プランジャ（スプリングボールプランジャ）
１９ 支持体保持器
２１ レンズドーム
10
40 Display (CRT)
11 Body A
12 Body B
13
19 Support holder 21 Lens dome
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| JP2024039083A (en) * | 2022-09-09 | 2024-03-22 | 株式会社ナリス化粧品 | Skin condition feature estimation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2024039083A (en) * | 2022-09-09 | 2024-03-22 | 株式会社ナリス化粧品 | Skin condition feature estimation method |
| JP7853060B2 (en) | 2022-09-09 | 2026-04-28 | 株式会社ナリス化粧品 | Method for estimating skin condition features |
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