JP2007040814A - Absorbance measuring sensor and method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an absorbance measuring sensor and an absorbance measuring method capable of obtaining a precise measured result over a wide range from a low value of absorbance up to a high value thereof. <P>SOLUTION: The first measuring chamber 14 having the largest volume is arranged in a position nearest to an injection port 12, the second measuring chamber 15 having the second-large volume is connected consecutively to the first measuring chamber, the third measuring chamber 16 having the third-large volume is connected consecutively to the second measuring chamber, and the fourth measuring chamber 17 having the smallest volume is connected consecutively to the third measuring chamber 16. The same specimen is measured by the plurality of measuring chambers different in heights, and the absorbance of a change lowest in the absorbance to a transmittance is selected, based on an absorbance measured result to attain the precise measurement. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to an absorbance measurement sensor and an absorbance measurement method for reacting a specimen with a reagent and measuring the absorbance of the reacted specimen.

  Conventionally, as shown in FIG. 12, the absorbance measurement sensor has an inlet 2 for injecting a specimen into the sensor body 1, a flow path 3 for transferring the injected specimen, and the absorbance of the transferred specimen. And a measurement chamber 4 for measuring

  Further, as shown in FIG. 13, the measuring apparatus using the absorbance measuring sensor includes a rotating unit 8 for rotating the sensor body 1, a light source 5 for irradiating the measurement chamber 4 with light 6, and a measurement. The light receiving unit 7 is configured to receive the light 6 transmitted through the chamber 4 and convert it into an electrical signal, and the signal processing unit 8 that processes the electrical signal from the light receiving unit 7.

  In the measurement method, a sample is first poured into the injection port 2, a sample having a difference in specific gravity is separated by the rotation of the rotating unit 9, and the separated sample is filled in the measurement chamber 4. Then, the light source 5 irradiates the measurement chamber 4 filled with the specimen with the light 6. The irradiated light 6 passes through the specimen, is received by the light receiving unit 7, converted into an electrical signal, and sent to the signal processing unit 8. The signal processing unit 8 processes electric signals and performs quantitative measurement of each component in the specimen.

For example, Patent Document 1 and Patent Document 2 are known as prior art document information relating to the invention of this application.
JP-A-3-225276 JP-A-2-269969

  As a method for quantitatively measuring each component of the specimen, it can be obtained by reacting the component to be measured with a reagent, irradiating the reacted specimen with light, and measuring the light absorption degree (absorbance). The absorbance ABS at that time is expressed by Equation (1) where Io is incident light and I is outgoing light.

ABS = log (Io / I) ......... Formula (1)
Here, when the change in absorbance of the sample to be measured is 0 to 2, for example, and Io is 1, I changes 1 to 0.01 and 100 times. At this time, if the coefficient of variation CV is set to 3% or less, the standard deviation σ = ± 0.0006 is obtained at the absorbance 0.02, and I is 0.95499 and σ = ± 0.00132. Further, at absorbance 2, σ = ± 0.06, and I is I = 0.01, σ = + 0.00148, and −0.00129.

  Thus, when trying to ensure accuracy, it is necessary for the apparatus side to read minute changes in I, particularly in the low and high absorbance regions. However, the stability of the amount of light emitted from the light source, the detection sensitivity and detection accuracy of I have finite values depending on the accuracy of components, temperature changes, and circuit noise levels, and it is difficult to raise them extremely. In this case, the apparatus side needs to lower the CV or narrow the absorbance range in which a desired CV can be secured.

  The present invention solves the above-described conventional problems, and an object thereof is to provide an absorbance measurement sensor and an absorbance measurement method capable of obtaining a highly accurate measurement result in a wide range from a low absorbance value to a high absorbance value.

  In order to solve the above-mentioned conventional problems, the absorbance measurement sensor according to the present invention analyzes the transmitted light generated by the light irradiated on the specimen contained in the measurement chamber provided on the disk passing through the specimen. In the absorbance measurement sensor for measuring the absorbance of the specimen, the measurement chamber includes a plurality of chambers having different optical path lengths of the transmitted light, and is optimal for measurement among the plurality of chambers having different optical path lengths of the transmitted light. Selection means for selecting a chamber is provided, and the absorbance of the specimen is measured by analyzing the transmitted light transmitted through the specimen contained in the chamber selected by the selection means.

  Furthermore, the absorbance measurement sensor of the present invention includes an injection chamber for receiving the sample, and the sample received by the injection chamber is transferred to the measurement chamber through a flow path.

  Furthermore, in the absorbance measurement sensor of the present invention, a plurality of flow paths formed by branching the flow paths are formed, and the plurality of flow paths are provided in all chambers having different optical path lengths of the transmitted light constituting the measurement chamber. Through the channel, the specimen is transferred from the injection chamber to each chamber.

  Furthermore, the absorbance measurement sensor of the present invention is configured such that all chambers having different optical path lengths of the transmitted light constituting the measurement chamber are integrally formed.

  Furthermore, the absorbance measurement sensor of the present invention is formed by cascading all the chambers having different optical path lengths of the transmitted light that constitute the measurement chamber via the flow path.

  Furthermore, the absorbance measurement sensor of the present invention is configured so that all the chambers constituting the measurement chamber having different optical path lengths of the transmitted light are separated from the injection chamber in order from the chamber in which the volume of the portion containing the specimen is large. The channel length is arranged at a short position.

  Furthermore, in the absorbance measurement sensor according to the present invention, the selection means has a minimum change in absorbance with respect to the transmittance of the sample contained in the chamber among the plurality of chambers having different optical path lengths of the transmitted light in the measurement chamber. Select the chamber to be

  Furthermore, in the absorbance measurement sensor of the present invention, the selection means is within an absorbance range in which the change in absorbance with respect to the transmittance is determined based on the optical path length of the chamber and the molar extinction coefficient of the specimen. Among the chambers, a chamber having a measured absorbance within the absorbance range is selected.

  Further, the absorbance measurement sensor according to the present invention is configured such that the plurality of chambers having different optical path lengths of the transmitted light are configured such that the specimens to be accommodated can be transferred to each other via the flow path, and the disk is further rotated. A control means for controlling, wherein the measured absorbance is determined based on the optical path length of the chamber and the molar extinction coefficient of the sample, and when the change in absorbance with respect to the transmittance is not within the smallest absorbance range, the selection The means changes the selected chamber and selects another chamber having a different optical path length of the transmitted light, and the control means controls the rotation of the disk to transfer the specimen to the other chamber. .

  Furthermore, in the absorbance measurement sensor of the present invention, the light irradiated to the specimen housed in the measurement chamber composed of a plurality of chambers provided on the disk and having different optical path lengths of the transmitted light is transmitted through the specimen. In the absorbance measurement method for analyzing the transmitted light and measuring the absorbance of the specimen, the first step of measuring the absorbance in a plurality of chambers having different optical path lengths of the transmitted light, and the absorbance measured in the first step A second step of determining whether or not a change in absorbance with respect to transmittance among the plurality of chambers is measured by a chamber having the smallest absorbance, and a chamber having the smallest change in absorbance with respect to transmittance in the absorbance measured in the first step. And a third step of outputting a measurement value.

  Furthermore, in the absorbance measurement sensor of the present invention, the light irradiated to the specimen housed in the measurement chamber composed of a plurality of chambers provided on the disk and having different optical path lengths of the transmitted light is transmitted through the specimen. In the absorbance measurement method for analyzing the transmitted light and measuring the absorbance of the specimen, the first step of measuring the absorbance in the first chamber among the plurality of chambers having different optical path lengths of the transmitted light, and the measurement in the first step A second step of determining whether the chamber having the smallest change in absorbance with respect to the transmittance among the plurality of chambers is the first chamber; and the transmittance in the absorbance measured in the first step. A third step of outputting a measurement value of the first chamber when the chamber having the smallest change in absorbance with respect to the first chamber is the first chamber; That.

  According to the present invention, it is possible to provide an absorbance measurement sensor and an absorbance measurement method capable of obtaining a highly accurate measurement result in a low absorbance region and a high absorbance region.

(Embodiment 1)
Hereinafter, an absorbance measurement sensor and an absorbance measurement method according to Embodiment 1 of the present invention will be described with reference to the drawings.

  FIG. 1 shows an overview of the absorbance measurement sensor according to the present embodiment.

  In FIG. 1, reference numeral 11 denotes a sensor body, and the sensor body 11 is formed of a transparent or translucent material. Reference numeral 12 denotes an injection chamber for injecting a specimen formed inside the sensor body 11, and a hole for specimen injection is provided in the whole or a part of the upper surface. Reference numeral 14 denotes a first measurement chamber, 15 denotes a second measurement chamber, 16 denotes a third measurement chamber, and 17 denotes a fourth measurement chamber.

  Reference numeral 13 denotes a flow path A for transferring the injected specimen to the measurement chamber, one of which is connected to the injection chamber 12 and the other branching into four, the first measurement chamber 14 and the second measurement chamber 15. Are connected to the third measurement chamber 16 and the fourth measurement chamber 17, respectively. The height of the first measurement chamber is 3, the height of the second measurement chamber is 2, the height of the third measurement chamber is 1, and the height of the fourth measurement chamber is 0.5. The measurement chamber is arranged concentrically from the center of the sensor body 11.

  FIG. 2 shows an overview of another absorbance measurement sensor according to the present embodiment.

In FIG. 2, 19 is a measurement chamber in which a first measurement chamber 14, a second measurement chamber 15, a third measurement chamber 16, and a fourth measurement chamber 17 are integrated, and 18 is an injection chamber 12 and a measurement chamber. It is the flow path B which connects 19 one-on-one.
Other sensors for measuring absorbance are the ones that integrate the four measurement chambers of the sensor for measuring absorbance, and the operation is exactly the same as the sensor for measuring absorbance. There is a merit that can be done.

  FIG. 3 is a cross-sectional view of the measurement chamber.

  In FIG. 3, 21 is a chamber having a height L, and 20 is a specimen having a molar extinction coefficient ε filled in the chamber 21.

  Here, when the solution concentration is C, the relationship between the optical path length L and the absorbance ABS in the solution is expressed by Equation (2). Furthermore, the transmittance is expressed by equation (3) from equations (1) and (2).

ABS = εCL ......... Formula (2)
I / Io = 10 ⁻ ε ^CL = 10− ^ABS Equation (3)
Here, if ε = 1, L = 0.5, 1, 2, 3 and C changes from 0 to 2, Equation (3) is graphed as shown in FIG.
4 is an enlarged graph of the low absorbance region in FIG. 4, and FIG. 6 is an enlarged graph of the high absorbance region.

  In measuring the absorbance, the outgoing light I by absorption is measured, and the absorbance is calculated using the incident light Io measured in advance and the equation (2). In such a case, in order to measure the absorbance more accurately, it is necessary to increase the measurement accuracy of the transmittance. However, as described above, the stability of the amount of light emitted from the light source and the detection of I Sensitivity and detection accuracy have finite values depending on component accuracy, temperature change, and circuit noise level, and are difficult to raise extremely. However, even when the fundamental transmittance measurement accuracy is the same, it is possible to increase the measurement accuracy of the final absorbance by reducing the change in absorbance relative to the transmittance in the process of converting the transmittance to absorbance. It is.

  In FIG. 5, among the four graphs, the graph with L = 3 shows the smallest change in absorbance with respect to the transmittance. From this, in the region where the absorbance is low, the measurement accuracy of the absorbance can be increased by using the measurement chamber of L = 3 rather than the measurement chambers of L = 0.5, 1, and 2.

  In FIG. 6, among the four graphs, the graph with L = 0.5 has the smallest change in absorbance with respect to the transmittance. For this reason, in the region where the absorbance is high, it is possible to increase the measurement accuracy of the absorbance by using the measurement chamber with L = 0.5 rather than the measurement chamber with L = 1,2,3.

  Here, the change in absorbance with respect to the transmittance is represented by the slope of the graph. In FIGS. 4, 5, and 6, the greater the slope, the smaller the change in absorbance with respect to the transmittance and the better the measurement accuracy of absorbance.

  FIG. 7 is a graph showing the absolute value of the slope of the transmittance with respect to the absorbance obtained by differentiating the graph of FIG. 4 to obtain the absolute value.

  In FIG. 7, it can be seen that the absorbance range in which the slope increases in the four graphs is different. L = 3 at an absorbance of 0 to 0.15, L = 2 at an absorbance of 0.15 to 0.3, L = 1 at an absorbance of 0.3 to 0.6, and L = 0. 5 shows that the slope is the largest compared to the other graphs. From this, the absorbance value taken by the measurement object can be measured more accurately by measuring in the chamber having the optical path length of the graph having the largest slope.

Hereinafter, the measurement apparatus in the present embodiment will be described with reference to FIG.
In FIG. 8, 22 is a light source having directivity, 23 is a moving unit for moving the light source 22 in the inner and outer peripheral directions of the sensor body 11, 24 is a rotating unit for rotating the sensor body 11, and 25 is emitted from the light source 22. Light, 26 is a light receiving unit that receives the light 25 transmitted through the sensor body 11 and converts it into an analog electric signal, 27 is an amplifier that amplifies the analog electric signal from the light receiving unit 26, and 28 converts the analog electric signal into digital data. ADC 29 for calculating the absorbance using digital data from the ADC 28, 30 for referring to the result of the absorbance calculator 29, and selecting the optimum value from among them, 31 for the result It is a display unit that displays the value selected by the selection unit 31.

First, the sample to be measured is injected into the injection chamber 12 with a pipette or the like. Thereafter, the sensor 11 is set on the rotating unit 24. After the setting, the sensor 11 rotates at a predetermined rotational speed, and the specimen passes through the flow path A13 by the centrifugal force, and the first measurement chamber 14, the second measurement chamber 15, the third measurement chamber 16, and the fourth measurement. Equally transferred to the chamber 17.
Here, the reagent is applied in the flow path A13 or a reagent chamber (not shown), and a chemical reaction such as a color reaction or an agglutination reaction occurs when the specimen passes therethrough. If necessary, a centrifuge chamber is provided in the flow path A13, and when the sample is in the centrifuge chamber, the rotating unit 24 rotates at a high speed to separate samples having different specific gravity and extract necessary components. It is also possible.

  It is also possible to provide filter paper or a filter in the injection chamber 12 or the flow path A13 and extract only necessary components. Further, if the height of the sample liquid surface varies, a difference occurs in the optical path length and an error occurs in the absorbance. Therefore, it is necessary to always maintain a constant height. For this purpose, the structure is such that the measurement chamber is always filled with centrifugal force while maintaining a rotation of a predetermined number of rotations or more, or an overflow chamber is provided and flows into the chamber when the height exceeds a certain level. Thus, it is necessary to keep the specimen height constant.

  The light source 22 can be moved in the inner and outer peripheral directions of the sensor main body 11 by the moving unit 23, and in measurement, the first light source 22 is arranged concentrically from the center of the sensor main body 11 rotating at a predetermined number of rotations. The measurement chamber 14, the second measurement chamber 15, the third measurement chamber 16, the fourth measurement chamber 17, and a reference chamber (not shown) are located immediately below, and the same amount of light is applied to all measurement chambers and a reference chamber (not shown). The light 25 is irradiated.

  Here, the reference chamber (not shown) is for measuring the amount of incident light Io as a reference for calculating the absorbance, and the chamber is filled with air or a substance having the same refractive index as the specimen. Yes.

  The irradiated light 25 passes through all measurement chambers and reference chambers filled with the specimen, and the transmitted light is received by the light detection unit 26. The light received by the light detection unit 26 is converted into an electrical signal, amplified by an amplifier 27 to a level that can take a dynamic range necessary for measurement, and input to the ADC 28. The electrical signal input to the ADC 28 is converted into digital data and sent to the absorbance calculation unit 29.

Here, the flow from ADC data acquisition to result display will be described with reference to FIG.
9, the absorbance calculation section 29, transmitted light quantity I ₁₄ of the first measurement chamber 14 transmitted as digital data from the ADC 28, the transmitted light quantity I ₁₅ of the second measuring chamber 15, transmission of the third measurement chamber The light amount I ₁₆ , the transmitted light amount I ₁₇ of the fourth measurement chamber, and the transmitted light amount Io of the reference chamber are received. Here, the transmitted light amounts I ₁₄ to _{17 of} each measurement chamber and the transmitted light amount Io of the reference chamber correspond to I and Io in the equation (2), respectively. It is represented by (4), formula (5), formula (6), and formula (7). The absorbance calculation unit 29 calculates the first measurement chamber 14, the second measurement chamber 15, the third measurement chamber 16, the fourth measurement from the expressions (4), (5), (6), and (7). The absorbances ABS ₁₄ , ABS ₁₅ , ABS ₁₆ , and ABS ₁₇ of each measurement chamber 17 are calculated and transferred to the result selection unit 30.

ABS ₁₄ = 1/3 * log (Io / I ₁₄ ) Equation (4)
ABS ₁₅ = 1/2 * log (Io / I ₁₅ ) (5)
ABS ₁₆ = log (Io / I ₁₆ ) ……… Formula (6)
ABS ₁₇ = 2 * log (Io / I ₁₇ ) ......... Formula (7)
Next, the result selection unit 30 checks whether or not the ABS ₁₄ is in the range of 0 to 0.15, and if it is within the range, sends the calculated ABS ₁₄ to the display unit 31. If it is out of range, it is checked whether or not ABS ₁₅ is in the range of 0.15 to 0.3, and if it is within range, the calculated ABS ₁₅ is sent to the display unit 31. If it is outside the range, it is checked whether or not the ABS ₁₆ is in the range of 0.3 to 0.6. If it is within the range, the calculated ABS ₁₆ is sent to the display unit 31. If it is still out of range, the ABS ₁₇ is unconditionally sent to the display unit 31.

  As described above, in the present embodiment, the same specimen is measured in a plurality of measurement chambers having different heights, and the absorbance value obtained in the measurement chamber having a small change in absorbance with respect to the transmittance is selected from the absorbance results. By doing so, measurement with higher accuracy becomes possible.

(Embodiment 2)
FIG. 10 is an overview of the absorbance measurement sensor according to Embodiment 2 of the present invention. In the second embodiment, the same components as those in the first embodiment are denoted by the same reference numerals, and the description is simplified.

  In FIG. 10, reference numeral 32 denotes a flow path C having a siphon structure in which measurement chambers are cascade-connected and a specimen is transferred by capillary force. In addition, the first measurement chamber 14 having the largest volume is disposed at the position closest to the inlet 12, and then the second measurement chamber 15 having the second largest volume is connected to the first measurement chamber, and then The third measurement chamber 16 having the third largest volume is connected to the second measurement chamber, and finally the fourth measurement chamber 17 having the smallest volume is connected to the third measurement chamber 14.

  Next, the operation will be described using the absorbance measurement flowchart of FIG.

  First, the specimen placed in the injection port 12 is sent to the first measurement chamber 14 by the centrifugal force generated by the rotation of the rotating unit 24. When the specimen is in the first measurement chamber 14, the light source 22 is moved immediately below the first measurement chamber 14 by the moving unit 23, and the first measurement chamber 14 is irradiated with light 25 there. The transmitted light is received by the light detection unit 26. At this time, in the sensor body 11, the light received by the light detection unit 26 is converted into an electric signal, amplified by the amplifier 27 to a level at which a dynamic range required for measurement can be taken, and input to the ADC 28.

The electrical signal input to the ADC 28 is converted into digital data and sent to the absorbance calculation unit 29, where the absorbance ABS _{14 is} calculated from the transmitted light amount I ₁₄ , the transmitted light amount Io of a reference chamber (not shown) obtained in advance and the equation (4). Is calculated. The calculated absorbance ABS ₁₄ is sent to the result selection unit 30. Next, the result selection unit 30 checks whether or not the ABS ₁₄ is in the range of 0 to 0.15, and if it is within the range, sends the calculated ABS ₁₄ to the display unit 31. When it is out of the range, the rotation unit 23 stops the rotation that has been rotated at the predetermined rotation number or reduces the rotation number. Accordingly, the capillary force exceeds the centrifugal force, and the specimen is sent from the first measurement chamber 14 to the second measurement chamber 15 through the flow path C32. After being sent, the rotating unit 24 rotates again at a predetermined rotational speed.

Next, the light source 22 moves directly below the second measurement chamber 15 and irradiates the light 25 as in the previous time to obtain the transmitted light amount I ₁₅ . The absorbance calculation unit 29 calculates the absorbance ABS ₁₅ using the transmitted light amounts I ₁₅ and Io and equation (5). The calculated absorbance ABS ₁₅ is sent to the result selection unit 30 as in the previous time. Next, the result selection unit 30 checks whether or not the ABS ₁₅ is in the range of 0.15 to 0.3, and if it is within the range, sends the calculated ABS ₁₅ to the display unit 31. When it is out of the range, the rotation unit 23 stops the rotation that has been rotated at the predetermined rotation number or reduces the rotation number. Thereby, the specimen is sent from the second measurement chamber 15 to the third measurement chamber 16 through the flow path C32.

Then light source 22 is moved directly below the third measurement chamber 16, the quantity of transmitted light I ₁₆ acquired, calculating the absorbance ABS ₁₆ by using the Io and Equation (6). The calculated absorbance ABS ₁₆ is sent to the result selection unit 30 as in the previous time. The result selection unit 30 checks whether or not the ABS ₁₆ is in the range of 0.3 to 0.6, and if it is within the range, sends the calculated ABS ₁₆ to the display unit 31. When it is out of the range, the rotating unit 23 stops the rotation that has been rotated at the predetermined number of rotations or reduces the number of rotations, and the specimen passes from the third measurement chamber 16 to the fourth measurement chamber 17 through the flow path C32. Sent.

Next, the light source 22 moves directly below the fourth measurement chamber 17, acquires the transmitted light amount I ₁₇ , and calculates the absorbance ABS ₁₇ using Io and Equation (7). The calculated absorbance ABS ₁₇ is sent to the result selection unit 30 as in the previous time. The result selection unit 30 sends the result to the ABS ₁₇ display unit 31.

  As described above, in the present embodiment, the same specimen is measured in a plurality of measurement chambers having different heights, and the absorbance value obtained in the measurement chamber having a small change in absorbance with respect to the transmittance is selected from the absorbance results. By doing so, measurement with higher accuracy becomes possible. In addition, since the sample is moved and sequentially measured, the amount of the sample is much smaller than that of the first embodiment.

  According to the absorbance measurement sensor, measurement apparatus, and measurement method according to the present invention, the measurement error can be reduced by selecting a measurement chamber having an optical path length with a small change in absorbance with respect to the transmittance in the absorbance value of the measurement object. Since it can be reduced, it can be applied to a highly accurate absorbance measuring apparatus.

Overview of absorbance measurement sensor according to Embodiment 1 of the present invention Overview of another absorbance measurement sensor according to Embodiment 1 of the present invention Sectional drawing of the measurement chamber in Embodiment 1 of this invention Absorbance vs. transmittance graph in Embodiment 1 of the present invention Absorbance (low absorbance value) vs. transmittance graph in Embodiment 1 of the present invention Absorbance (absorbance high value) vs. transmittance graph in Embodiment 1 of the present invention Absorbance vs. transmittance slope graph in Embodiment 1 of the present invention Measuring device block diagram in Embodiment 1 of the present invention Absorbance measurement flowchart in Embodiment 1 of the present invention Overview of absorbance measuring sensor according to Embodiment 2 of the present invention Absorbance measurement flowchart in Embodiment 2 of the present invention Overview of conventional absorbance measurement sensor Block diagram of a conventional absorbance measurement device

Explanation of symbols

11 Sensor body 12 Injection chamber 13 Flow path A
14 First measurement chamber 15 Second measurement chamber 16 Third measurement chamber 17 Fourth measurement chamber 18 Flow path B
19 Integrated measurement chamber 20 Sample 21 Measurement chamber 22 Light source 23 Moving unit 24 Rotating unit 25 Light 26 Light receiving unit 27 Amplifier 28 ADC
29 Absorbance calculation unit 30 Result selection unit 31 Display unit 32 Channel C

Claims (11)

In an absorbance measurement sensor for analyzing the transmitted light generated when light irradiated to a sample contained in a measurement chamber provided on a disk passes through the sample and measuring the absorbance of the sample, the measurement chamber transmits the transmission A plurality of chambers having different optical path lengths for light, and a selection means for selecting a chamber most suitable for measurement among the plurality of chambers having different optical path lengths for the transmitted light, and accommodated in the chamber selected by the selection means A sensor for measuring absorbance obtained by analyzing transmitted light transmitted through the sample and measuring the absorbance of the sample. The absorbance measurement sensor according to claim 1, further comprising an injection chamber that receives the specimen, wherein the specimen received by the injection chamber is transferred to the measurement chamber through a flow path. A plurality of flow paths formed by branching the flow paths are formed, and the analyte is chambered from the injection chamber to all the chambers having different optical path lengths of the transmitted light constituting the measurement chamber through the plurality of flow paths. The absorbance measurement sensor according to claim 2, wherein the absorbance measurement sensor is transferred every time. The absorbance measurement sensor according to claim 3, wherein all chambers having different optical path lengths of the transmitted light constituting the measurement chamber are integrally formed. The absorbance measurement sensor according to claim 2, wherein all chambers having different optical path lengths of the transmitted light constituting the measurement chamber are cascade-connected through a flow path. All the chambers having different optical path lengths of the transmitted light constituting the measurement chamber are arranged in a position where the flow path length from the injection chamber is short in order from the chamber having the larger volume of the portion for accommodating the specimen. The absorbance measurement sensor according to claim 5. The said selection means selects the chamber from which the change of the light absorbency with respect to the transmittance | permeability of the test substance accommodated in the said chamber becomes the minimum among several chambers from which the optical path length of the said transmitted light in the said measurement chamber differs. The absorbance measurement sensor according to 1. Among the chambers in which the selection means is within the absorbance range in which the change in absorbance with respect to the transmittance is determined based on the optical path length of the chamber and the molar extinction coefficient of the specimen, the measured absorbance is the absorbance range. The absorbance measuring sensor according to claim 7, wherein a chamber in the chamber is selected. The plurality of chambers having different optical path lengths of the transmitted light are configured such that the specimens to be accommodated can be transferred to each other via the flow path, and further include control means for controlling the rotation of the disk, and the measured absorbance is If the change in absorbance with respect to transmittance is not within the smallest absorbance range determined based on the optical path length of the chamber and the molar extinction coefficient of the sample, the selection means replaces the chamber to be selected and transmits light. 8. The absorbance measurement sensor according to claim 7, wherein another chamber having a different optical path length is selected, and the control means controls the rotation of the disk to transfer the specimen to the other chamber. . Analyzing the transmitted light generated by the light irradiated on the specimen housed in the measurement chamber composed of a plurality of chambers with different optical path lengths of the transmitted light provided on the disk, and measuring the absorbance of the specimen. In the absorbance measurement method to be measured, a first step of measuring absorbance in a plurality of chambers having different optical path lengths of the transmitted light, and a change in absorbance with respect to transmittance of the plurality of chambers measured in the first step. A second step of determining whether or not is measured by the smallest chamber, and a third step of outputting the measured value of the chamber having the smallest change in absorbance with respect to the transmittance in the absorbance measured in the first step. An absorbance measurement method comprising: Analyzing the transmitted light generated by the light irradiated on the specimen housed in the measurement chamber composed of a plurality of chambers with different optical path lengths of the transmitted light provided on the disk, and measuring the absorbance of the specimen. In the absorbance measurement method to be measured, a first step of measuring absorbance in a first chamber among a plurality of chambers having different optical path lengths of the transmitted light, and transmission of the plurality of chambers in the absorbance measured in the first step. A second step of determining whether or not the chamber having the smallest change in absorbance with respect to the rate is the first chamber; and the chamber having the smallest change in absorbance with respect to the transmittance in the absorbance measured in the first step. And a third step of outputting a measurement value of the first chamber when it is one chamber.

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