JP2006197863A - Culture medium for culturing lyophllum ulmarium - Google Patents

Culture medium for culturing lyophllum ulmarium Download PDF

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Publication number
JP2006197863A
JP2006197863A JP2005013679A JP2005013679A JP2006197863A JP 2006197863 A JP2006197863 A JP 2006197863A JP 2005013679 A JP2005013679 A JP 2005013679A JP 2005013679 A JP2005013679 A JP 2005013679A JP 2006197863 A JP2006197863 A JP 2006197863A
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culture medium
coconut
bunashimeji
bean
husk
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JP2005013679A
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Japanese (ja)
Inventor
Toshihiro Nishigata
Yasuo Ohira
Yoshinobu Ohira
Mitsuharu Yu
光治 勇
喜信 大平
安夫 大平
寿弘 西潟
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Yukiguni Maitake Co Ltd
株式会社雪国まいたけ
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Priority to JP2005013679A priority Critical patent/JP2006197863A/en
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Abstract

<P>PROBLEM TO BE SOLVED: To provide culture medium for culturing Lyophllum ulmarium obtained by utilizing sub-standard bean sprouts, beanstalks and the like treated as industrial wastes for part of culture medium for culturing Lyophllum ulmarium so as to ensure the same productivity as conventional one, solve industrial-waste problems becoming an object of public concern, and reduce costs for treating the wastes or load on the natural environment. <P>SOLUTION: The culture medium for culturing Lyophllum ulmarium is mixed with bean sprouts/beanstalks. The culture medium comprises at least sawdust, corn cob meal and bran, and is mixed with bean sprouts/beanstalks. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a culture medium for cultivation of bunashimeji mixed with bean sprouts and bean husks.
  Conventionally, a mixture of plant-derived raw materials obtained by mixing and agitating an appropriate amount of corn cobmeal, which is a pulverized product of corn cobs, and bran, which is wheat seed coat, together with water, is widely used as a culture medium for fungus bed of beech shimeji mushroom. Is used. This mixture is filled in about 850 ml of polypropylene bottles, and beech shimeji is grown in the medium.
  By the way, in sprouts production, non-standard sprouts, cut roots, sprouts of sprouts, etc. are generated in large quantities as residues, and these residues are treated as industrial waste.
  However, it is not preferable to treat the residue generated in the above-mentioned production of palm as industrial waste in terms of costs associated with waste treatment and a load on the natural environment.
  The present invention can ensure productivity equivalent to the conventional one by using non-standard sprout and sprout bean husk treated as industrial waste as a part of the culture medium for beech shimeji, It is an object of the present invention to provide a culture medium for cultivating beech shimeji which can solve the problem of industrial waste that is a problem and can reduce the cost of waste treatment and the load on the natural environment.
  The gist of the present invention will be described.
  The present invention relates to a culture medium for cultivating beech shimeji mushroom, which is mixed with bean sprouts.
  In addition, the present invention relates to a culture medium for cultivating beech shimeji consisting of at least sawdust, corn cobmeal and bran, wherein coconut bean husk is mixed.
  The culture medium for cultivation of bunashimeji according to any one of claims 1 and 2, wherein the bean sprout coconut shell is a dried product or an undried product. It is.
  The culture medium for cultivation of bunashimeji according to any one of claims 1 to 3, wherein the bean sprout coconut shell is a pulverized product or an unground product. It is.
  Moreover, in the culture medium for beech shimeji mushroom according to any one of claims 2 to 4, sawdust and corn cobmeal function as a support, and bran functions as a nutrient. Relates to a culture medium for cultivating Bunashimeji, which functions as a support or a nutrient.
  A culture medium for cultivating beech shimeji mushroom according to claim 5, wherein a maximum of 12% (dry weight) of sawdust and corn cobmeal is replaced with coconut palm coconut shell. It is concerned.
  In addition, in the culture medium for bunashimeji according to any one of claims 5 and 6, a maximum of 13% (dry weight) of the bran is replaced with bean sprouts and coconut bean husks. It concerns the medium.
  The culture medium for cultivation of bunashimeji according to any one of claims 5 to 7, wherein the dry weight ratio between the support and the nutrient is 1: 1 to 10: 1. This relates to the culture medium for
  The culture medium for bunashimeji according to any one of claims 1 to 8, wherein the coconut palm coconut shell is a mixture of coconut palm and coconut bean husk or coconut bean husk. It concerns a culture medium for cultivation.
  The cultivation medium for bunashimeji according to claim 9, wherein the bean sprouts and bean husks are 4: 1 to 0: 5 as a dry weight ratio of the bean sprouts and coconut bean husks. It concerns the medium.
  The present invention uses non-standard sprout or sprout bean husk treated as industrial waste as a part of the culture medium for beech shimeji, so it solves the industrial waste problem and costs associated with waste processing. It is possible to reduce the burden on the natural environment, and further, it becomes a culture medium for cultivation of bunashimeji that can secure the same productivity of bunashhimeji as before.
  The culture medium for fungus bed cultivation of Bunashimeji according to the present invention is characterized in that a part of the usual cultivation medium for fungus bed cultivation of Bunashimeji is replaced with bean sprouts.
  Therefore, the present invention uses non-standard sprout and sprout bean husk treated as industrial waste as a part of the culture medium for beech shimeji so that it can solve the industrial waste problem for waste disposal. It becomes the culture medium for cultivation of bunashimeji which can reduce the cost and the load with respect to a natural environment which concerns, and also can ensure the productivity of bunashimeji equivalent to the past.
  This example is a culture medium for cultivation of fungus bed of Bunashimeji comprising a support, nutrients, and bean sprouts.
  Hereinafter, the culture medium for cultivating the bed of beech shimeji mushroom in this example will be described in detail.
  Sawdust and corn cob meal are used as the support for the culture medium of this example.
  It is preferable to use hardwood sawdust as sawdust. In addition to hardwood sawdust, coniferous sawdust, waste hodwood grown on mushroom raw wood, and waste fungus bed after mushroom fungus bed cultivation can also be used.
  Corn cob meal is a crushed product of corn cobs.
  Since sawdust and corncob meal are organic substances, they have a function as a support as well as a function as a support.
  In addition to the above, a support that retains the medium voids, has water retention, and has little nutritional effect may be mixed.
  Moreover, bran is used as a nutrient of the culture medium of a present Example.
  The bran is wheat seed coat.
  The bran also has a function as a support due to its own volume.
  In addition, you may mix the rice bran, bean hull, corn bran, beer lees, okara, etc. as a nutrient alone or in a mixture of two or more.
  In addition to the above, the cultivation medium of a present Example is mixed with sprout and sprout bean husk, and the sprout and sprout bean husk functions as a support or a nutrient. A lot of fiber is contained in a sprout and a sprout bean husk, and this fiber can become a skeleton (support) in the culture medium for bunashimeji, and can also become a substrate (nutrient) in the culture medium for bunashimeji.
  In addition, the ratio of the support and the nutrient in this example varies depending on the particle size and density of the support and the nutrient used, and the container used. For example, hardwood sawdust and corn cob meal as the support, In the case of using bran as a product, the weight ratio is preferably 1: 1 to 10: 1, more preferably 2: 1 to 4: 1. It has been confirmed that the yield of Bunashimeji is reduced when the ratio of the support to the nutrient is other than this.
  The coconut palm coconut shell of this example is a residue such as non-standard coconut palms, cut roots, and coconut husks produced in the production of coconut palm, which may be either dried or undried products, The residue may be either a pulverized product or an unpulverized product, but these residues contain 90% or more of moisture and are susceptible to spoilage. At this time, a harmful substance with a strong irritating odor is produced, and to the medium. In this example, it is a dry product.
  This bean sprouts are dried using a dryer using waste heat from a boiler used in a factory or a normal hot air dryer.
  In the present embodiment, the residue is cut to about 5 mm for easy handling. The shape is not particularly limited, but it is easy to handle if it is cut to at least about 5 mm or less.
  Moreover, the sprout and sprout bean husk used in the present example is specifically a mixture of sprout and sprout bean husk or a sprout bean husk, and the dry weight ratio of sprout and sprout bean husk is 4: 1 to 0: 5. It is preferable that
  In addition, the maximum 12% (dry weight) of sawdust and corn cob meal contained in the culture medium of this example can be replaced with sprouts and sprouts. It has been confirmed that the yield of Bunashimeji is reduced when more than 12% (dry weight) of sawdust and corn cobmeal in the cultivation medium are replaced with bean sprouts.
  Moreover, the maximum 13% (dry weight) of the bran contained in the cultivation medium of the present embodiment can be replaced with sprouts. It has been confirmed that when the bran in the cultivation medium is replaced with more than 13% (dry weight) of bean sprouts, the yield of bunashimeji is reduced.
  In this example, after adjusting the water content of the cultivation medium containing sprout and sprout bean husk to 50 to 80% as described above, it is filled into an 850 ml bottle, and then this medium. The 850 ml bottle filled with is sterilized with a high-pressure or normal-pressure sterilization kettle or the like, allowed to cool, and then the short culture of Bunashimeji (used strain: Takara No. 1) on the cooled medium. Aseptically inoculated and spread the mycelium over the medium for 90 days under the conditions of temperature 20-30 ° C. and humidity 50-70%, then remove the stopper and gently press the center of the inoculum with a dish The inoculum part was left in the shape of a mountain by scraping, and about 10 ml of water was added thereto, and Buna shimeji was harvested by generating fruit bodies under conditions of a temperature of 10 to 20 ° C. and a humidity of 85% or more (central part of the inoculum) While gently pressing the Leaving the seed part to the mountain type by Nikki come, the operation of adding it to the water 10ml back and forth hereinafter, referred to as a scratching bacteria.).
  In addition to the fungus bed cultivation method by bottle cultivation of this example, the fungus bed cultivation method using bag cultivation or the like as a container can be cited as the cultivation method of beech shimeji, and the temperature, humidity, etc. are controlled as the form of cultivation. However, the present embodiment is not limited to these forms and systems. However, the present embodiment is not limited to these forms and methods.
  The strain of Bunashimeji used may be a commercially available strain (for example, Takara No. 1), a strain collected from a natural fruiting body, or a newly bred strain.
  In this example, non-standard sprout and sprout bean husk treated as industrial waste was used as a part of the cultivation medium for bunashimeji, which is the next production amount after Shiitake mushrooms and enoki mushrooms. By solving this problem, it is possible to reduce the cost related to waste disposal and the load on the natural environment, and further, it becomes a culture medium for cultivation of beech shimeji that can secure the same productivity of bun shimeji mushroom as before.
  Hereinafter, experimental examples that more specifically show the effects of the present embodiment will be described.
Experimental example 1
In Experimental Example 1, in the culture medium for fungus bed of beech shimeji consisting of hardwood beech, oak etc. sawdust, corn cobmeal and bran, when a part of sawdust and corn cobmeal is replaced with sprouts The effect of the culture medium for cultivating fungus bed on Bunashimeji on the yield of Bunashimeji is shown.
  Specifically, in Experimental Example 1, as shown in Table 1, sprout, sprout bean husk, sawdust and corn cob meal were mixed in a dry weight of 0 g, 65 g and 78 g, respectively, in a control group, 4.2 g, 60 g and 72 g. A mixed 4.2 g section, 8.4 g, 58 g and 70 g mixed 8.4 g section, and 16.7 g, 54 g and 65 g mixed 16.7 g section were prepared, respectively, and 60 g (Nutrition) is mixed and adjusted so that the water content becomes 65%. Each of the medium preparations thus prepared is packed in a polypropylene 850 ml wide-mouth bottle, 48 pieces, and a hole of about 1 cm is formed in the center. After opening, it was stoppered to make a fungus bed medium.
  In addition, the sawdust and corn cobmeal in Experimental Example 1 are 0% (dry weight) in the control group, 3.08% (dry weight) in the 4.2 g group, and 8.4 g group in the palm group. 6.15% (dry weight) and 12.3% (dry weight) are substituted in the 16.7g section.
  Next, the microbial bed medium was sterilized in a high-pressure sterilization pot at 120 ° C. for 60 minutes. After the sterilization was completed, the medium was allowed to cool and inoculated with Buna shimeji inoculum. This was cultured for 90 days in a culture room at a temperature of 23 ° C. and a humidity of about 50 to 70%, then the stopper was removed and the bacteria were scraped.
  Next, this was moved to a generation room having a temperature of 15 ° C. and a humidity of 90 to 100%, and the yield per bottle of buna shimeji that was harvested by generating fruit bodies under conditions of illuminance of 1000 lux or less was examined (Table 1). .
  According to Table 1 above, in all of the 4.2 g, 8.4 g and 16.7 g groups, the yield of bunashimeji is equivalent to that of the control group to which no sprout or sprout husk is added. The number of days from scraping to harvesting was also the same as the control.
  Therefore, even when up to 12.3% (dry weight) of hardwood beech and oak sawdust and corn cob meal are replaced with sprout and sprout bean hulls, the productivity of the same beech shimeji in the same number of days as before It was confirmed that
Experimental example 2
Experimental Example 2 shows that when a part of the bran is replaced with sprout and sprout husk in a medium for cultivation of fungus bed of beech shimeji consisting of hardwood beech, oak, etc., corn cob meal and bran, It shows how the culture medium for floor cultivation affects the yield of Bunashimeji.
  Specifically, as shown in Table 2, Experimental Example 2 is a control group in which 0 g and 60 g were mixed as a nutrient, and 4 g and 8 g in which 4 g and 56 g were mixed. And 8 g section mixed with 52 g and 16 g section mixed with 16 g and 44 g, respectively, and after mixing each of these nutrients with a support made of sawdust and corn cob meal in the amounts shown in Table 2, The water content was adjusted to 65%, and each of the medium preparations prepared in this way was packed into polypropylene 850 ml wide-mouthed bottles, 64 pieces each, and the yield per bottle of beech shimeji harvested in the same manner as in Experimental Example 1 was obtained. Investigated (Table 2).
  The bran of Experimental Example 2 is 0% (dry weight) in the control group, 6.65% (dry weight) in the 4g section, 13.3% (dry weight) in the 8g section, using the sprout and coconut husk. The 16g section is replaced with 26.6% (dry weight) and the 32g section is replaced with 53.2% (dry weight).
  According to Table 2 above, the yield of beech shimeji was the same in the 4 g and 8 g groups as that of the control group to which no sprout / coconut husk was added, but in the 16 g and 32 g groups, the yield of bun shimeji was obtained. Was clearly reduced. In addition, the days from fungus scraping to harvesting were the same as the control group in all the groups.
  Therefore, it was confirmed that even if a maximum of 13.3% (dry weight) of the bran was replaced with bean sprouts and bean husks, it was possible to obtain the same bunashimeji productivity with the same number of cultivation days as before.
Experimental example 3
Experimental Example 3 shows how the culture medium for fungus bed cultivation of beech shimeji mixed with various ratios of bean husk and bean sprout affects the yield of beech shimeji.
  Specifically, in Experimental Example 3, the ratio of sprout bean husk and sprout is mixed so that the ratio is 0: 5, 1: 4, 2.5: 2.5, 4: 1 and 5: 0. Yes.
  In Experimental Example 3, 58 g, 63 g and 60 g of sawdust, corn cobmeal and bran were mixed, respectively, and sprouts and sprouts were mixed at a dry weight ratio of 5 g and 0 g, respectively (0: 5). Mixed in a ratio of 4 g and 1 g (1: 4), mixed in 2.5 g and 2.5 g (2.5: 2.5) mixed in a ratio of 1 g and 4 g (4: 1) In addition, the control group in which the mixture was mixed at a ratio of 0 g and 5 g (5: 0), and the control group in which the sprout and the sprout husk were not mixed was prepared, and the water content was adjusted to 65%. Each of the prepared medium preparations was packed into a 850 ml wide-mouth bottle made of polypropylene, 48 pieces each, and the yield per bin of beech shimeji harvested in the same manner as in Experimental Examples 1 and 2 was examined (Table 3).
  According to Table 3 above, the yield of bunashimeji is (1: 4), (2.5: 2.5), (4: 1), and (5: 0) in sprouts In the (0: 5) group, the yield of Bunashimeji was reduced, although it was the same as the control group to which no shell was added. In addition, the days from fungus scraping to harvesting were the same as the control group in all the groups.
  Therefore, it has been confirmed that by combining the ratio of sprout and sprout bean husk so that at least the ratio of sprout bean husk does not become zero, the same productivity of buna shimeji can be obtained with the same number of cultivation days as before. It was.
Experimental Example 4
Experimental Example 4 shows how the presence / absence of drying and pulverization of sprout and sprout bean husk mixed in the culture medium for fungus bed cultivation of beech shimeji affects the yield of beech shimeji.
  Specifically, Experimental Example 4 is a dry / pulverized section in which the ratio of sprout and sprout bean husk is between 4: 1 and 0: 5, and then pulverized to about 5 mm after drying. An undried and unpulverized section that is not pulverized, an undried and pulverized section that is pulverized without drying, and a control section that is not mixed with bean sprouts and coconut bean husks are prepared. 5g each was mixed with 74g sawdust and 65g corn cob meal, and each of the medium preparations prepared in this way was packed into polypropylene 850ml wide-mouthed bottles, 16 each, and harvested in the same manner as in Experimental Examples 1-3. The yield per bottle of the beech shimeji was examined (Table 4).
  According to Table 4 above, the yield of Bunashimeji was equivalent to that of the control group in all the groups to which sprout and sprout husk were added. In addition, the days from fungus scraping to harvesting were the same as the control group in all the groups.
  Therefore, it is confirmed that bean-shimeji bean shimeji can be obtained in the same number of cultivation days as before, whether it is undried, unground or dried / ground. It was done.
  Therefore, if it is a fresh sprout and a sprout bean husk, it can be mixed in the culture medium for the fungus bed cultivation of Bunashimeji without the cost for drying and crushing.
  It should be noted that these residues generated in the production of sprouts contain 90% or more of moisture, are easily spoiled, and are difficult to add to the medium.

Claims (10)

  1.   A culture medium for cultivating beech shimeji mushroom, characterized by being mixed with bean sprouts.
  2.   A culture medium for cultivating beech shimeji comprising at least sawdust, corn cobmeal and bran, which is mixed with bean sprouts and coconut bean shells.
  3.   The culture medium for cultivating bunashimeji according to any one of claims 1 and 2, wherein the coconut palm husk is a dried product or an undried product.
  4.   The culture medium for cultivating beech shimeji mushroom according to any one of claims 1 to 3, wherein the bean sprouts or coconut bean shell is a pulverized product or an unground product.
  5.   The culture medium for bunashimeji according to any one of claims 2 to 4, wherein sawdust and corn cobmeal function as a support, bran functions as a nutrient, and mixed coconut palm husk is supported. A culture medium for cultivation of Bunashimeji, which functions as a body or a nutrient.
  6.   6. A culture medium for cultivating bunashimeji according to claim 5, wherein at most 12% (dry weight) of sawdust and corn cobmeal is replaced with coconut palm husk.
  7.   The culture medium for cultivation of bunashimeji according to any one of claims 5 and 6, characterized in that a maximum of 13% (dry weight) of the bran is replaced with bean sprouts.
  8.   The cultivation medium for bunashimeji according to any one of claims 5 to 7, wherein the dry weight ratio between the support and the nutrient is 1: 1 to 10: 1. Medium.
  9.   The culture medium for cultivation of bunashimeji according to any one of claims 1 to 8, wherein the coconut palm husk is a mixture of coconut palm and coconut husk or coconut beans husk. Culture medium.
  10. The culture medium for cultivating bun shimeji mushroom according to claim 9, wherein the dry coconut bean husk is 4: 1 to 0: 5 as a dry weight ratio between coconut coconut and coconut bean husk.
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