JP2005296778A - Method for removing heavy metal from mushroom extract - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for simply removing heavy metals from a mushroom extract at ordinary temperature. <P>SOLUTION: The method for removing heavy metals from a mushroom extract comprises bringing the mushroom extract into contact with a chelating fiber. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method for removing heavy metals from extracts of various mushrooms including Agaricus blazei.

Various mushrooms including Agaricus blazei are said to have a beneficial pharmacological action for the human body such as an immunostimulatory action. In recent years, the demand for mushroom extract containing β-glucan, which is considered to be an active ingredient, has been increasing. The method for producing a mushroom extract is based on extracting an extract from a mushroom cultivated using a solid medium or using a liquid medium using an extraction solvent such as water or hot water. Since mushrooms have the property of accumulating heavy metals such as cadmium, when the used medium contains heavy metals, the mushroom extract contains the heavy metals contained in the medium. Of course, the amount of heavy metal contained in the extract of mushrooms supplied to the market today is such that it does not affect the human body due to strict control of the amount of heavy metal contained in the medium used. Although attention is paid, it is desirable that the amount be reduced as much as possible in consideration of safety. Therefore, for example, in Patent Document 1 below, a method using apatite is proposed as a method for removing heavy metals from an extract of mushrooms.
JP 2001-259303 A

However, since the method described in Patent Document 1 has a preferable processing temperature as high as 70 ° C. to 100 ° C., the active ingredient contained in the extract may be deteriorated at high temperature.
Then, an object of this invention is to provide the method of removing a heavy metal simply from a mushroom extract at normal temperature.

The method for removing heavy metals from the mushroom extract of the present invention made in view of the above points is characterized in that, as described in claim 1, a treatment for bringing a chelate fiber into contact with the mushroom extract is performed.
The method according to claim 2 is characterized in that, in the method according to claim 1, the mushroom is Agaricus blazei.

  According to the present invention, a method for easily removing heavy metals from a mushroom extract at room temperature is provided.

  The chelate fiber used in the method for removing heavy metals from the mushroom extract of the present invention has a functional group having a chelate-forming ability for heavy metal ions such as cadmium ions introduced into the fiber substrate, Any material may be used as long as it has the property that it can be adsorbed and captured and removed from the system. Among commercially available chelate fibers, for example, Kirest Fiber (registered trademark) manufactured by Kirest Co., Ltd. can be suitably used.

  The treatment for bringing the chelate fiber into contact with the mushroom extract is, for example, adding the chelate fiber to the mushroom extract and stirring the mixture at a room temperature (15 ° C. to 30 ° C.) for a predetermined time and then absorbing and capturing the heavy metal. May be performed by solid-liquid separation from the extract by centrifugation or the like (batch feed treatment). Considering the efficiency and simplicity of treatment, the mushroom extract can be diluted with water as necessary to form a solid containing 10 w / v% to 30 w / v%, or the pH can be adjusted to 5-7. It is desirable to use for processing. In addition, the amount of chelate fiber added to the mushroom extract is different depending on the amount of the mushroom extract to be processed and the amount of heavy metals expected to be contained. It is desirable that it is 0.1 w / w%-20 w / w%. The stirring time is generally desirably 10 hours to 30 hours.

  The treatment of bringing the chelate fiber into contact with the mushroom extract is performed by passing the mushroom extract extract through a column filled with the chelate fiber, adsorbing and capturing heavy metals on the chelate fibers in the column, and removing the mushroom extract from which the heavy metals have been removed. You may carry out by collect | recovering (column liquid treatment). Considering the efficiency and simplicity of treatment, the mushroom extract can be diluted with water as necessary to form a solid containing 10 w / v% to 30 w / v%, or the pH can be adjusted to 5-7. In this case, it is desirable to use the processing in the same manner as in the batch feed processing.

  The mushroom extract may be prepared by a method known per se using an extraction solvent such as water or hot water.

  The mushroom extract from which heavy metals have been removed by the method of the present invention can be prepared and used in liquid preparations or granules in accordance with conventional methods.

  Hereinafter, the present invention will be described in more detail with reference to examples in which cadmium is removed from an extract of Agaricus blazei, but the present invention is not construed as being limited to the following description. In the following examples, 8 times the amount of water was added to dried Agaricus brazei fruit body, boiled with heating, extracted for 2 hours, filtered through a mesh of 60 mesh, and the obtained filtrate was decompressed at 60 ° C. or lower. Agaricus blazei extract (solid content 20 w / v%) prepared by concentration was used.

Example 1: Method by Batch Feed Processing Chelate fiber (Cylest) in each ratio of 0.1, 0.2, 0.3, 0.5, 1.0, and 2.0 w / v% to Agaricus blazei extract Fiber IRY: Kirest Fiber is a registered trademark of Kirest Co., Ltd. and stirred with a magnetic stirrer for 16 hours (pH of Agaricus brazei extract is 5.88, liquid temperature is 18 ° C.) at 3000 rpm for 10 minutes Centrifugation was performed for solid-liquid separation, and the supernatant was advantec-toyo no. Batch feed processing was performed by obtaining a filtrate by suction filtration using 5C filter paper.
The cadmium concentration was measured for each Agaricus blazei extract before and after the treatment. The cadmium concentration was measured using an atomic absorption photometer (polarized Zeeman atomic absorption spectrophotometer Z-7000 type: Hitachi, Ltd.) after acid decomposition of the sample. The measurement results are shown in the graph of FIG. As is clear from FIG. 1, the cadmium concentration of the extract before the treatment (untreated) was 1.60 ppm, but the cadmium concentration of the extract after the treatment was 0.06 ppm or less at all addition amounts, which was excellent. Cadmium removal effect was observed. In addition, even if this process was performed, the remarkable reduction | decrease of the quantity of (beta) -glucan contained in Agaricus blazei extract was not recognized.

Example 2: Method by column passage treatment Agaricus blazei extract was centrifuged at 3000 rpm for 10 minutes for solid-liquid separation, and the supernatant was added to ADVANTEC-TOYO No. 1 Suction filtration was performed using 5C filter paper, and the obtained filtrate was passed through a column cartridge (Kyrest Fiber IRY-C10: manufactured by Kyrest Co., Ltd.) filled with chelate fibers at a flow rate of 121 ml / min (filter before column passing). The pH of the liquid was 5.90, the liquid temperature was 21 ° C.), and the drainage liquid was collected to perform column flow treatment.
The cadmium concentration was measured in the same manner as in Example 1 for the Agaricus blazei extract before and after the treatment. The measurement results are shown in the graph of FIG. As apparent from FIG. 2, the cadmium concentration of the extract before treatment (untreated) was 1.15 ppm, but the cadmium concentration of the extract after treatment was 0.10 ppm, and an excellent cadmium removal effect was observed. It was. In addition, even if this process was performed, the remarkable reduction | decrease of the quantity of (beta) -glucan contained in Agaricus blazei extract was not recognized.

  The present invention has industrial applicability in that it can provide a method for easily removing heavy metals from a mushroom extract at room temperature.

3 is a graph showing the cadmium removal effect in Example 1. 5 is a graph showing the cadmium removal effect in Example 2.

Claims (2)

  A method for removing heavy metals from a mushroom extract, which comprises subjecting the mushroom extract to contact with a chelating fiber.   The method of claim 1, wherein the mushroom is Agaricus blazei.

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