JP2005130730A - Cell disrupter and, method for disrupting cell - Google Patents

Cell disrupter and, method for disrupting cell Download PDF

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JP2005130730A
JP2005130730A JP2003368172A JP2003368172A JP2005130730A JP 2005130730 A JP2005130730 A JP 2005130730A JP 2003368172 A JP2003368172 A JP 2003368172A JP 2003368172 A JP2003368172 A JP 2003368172A JP 2005130730 A JP2005130730 A JP 2005130730A
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storage cylinder
cooling
biological tissue
supply port
screw shaft
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Hitoshi Nagaoka
仁 長岡
Kenji Yamada
健二 山田
Masataka Morita
正隆 森田
Akio Nagata
明郎 永田
Tetsuya Kamataki
哲也 鎌滝
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Koki Holdings Co Ltd
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Hitachi Koki Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/08—Homogenizing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/20—Heating or cooling

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cell disrupter which can simply, quickly and perfectly freeze and disrupt tissue pieces including the liver, heart, and smooth muscle of an experimental animal or the like in high useful substance recovery and in good disruption efficiency. <P>SOLUTION: This cell disrupter that a screw shaft driven with a motor is rotatably supported in a receiving cylinder having a feeding port and that a fixed blade and a rotary blade for disrupting biological tissue pieces fed with the screw shaft are attached to the discharging side of the receiving cylinder is characterized by disposing a cooling tank for freezing the biological tissue pieces at a place near to the feeding port. The method for disrupting the cells is characterized by feeding frozen biological tissue pieces or the frozen biological tissue pieces and ice in a mixed state into a feeding port. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

本発明は生体組織を破砕することにより細胞または組織内の有用物質である酵素、ＤＮＡ、ＲＮＡ、タンパク質、抗がん性物質、細胞内微小組織等を取り出すことを目的とする細胞破砕装置に関し、取扱いが簡単で、大量の生態組織を迅速且つ安全に処理できるとともに、破砕効率、有用物質の回収率が高い細胞破砕装置に関する。   The present invention relates to a cell disruption device intended to extract enzymes, DNA, RNA, proteins, anticancer substances, intracellular micro-tissue, etc., which are useful substances in cells or tissues by disrupting living tissues, The present invention relates to a cell crushing apparatus that is easy to handle, can process a large amount of ecological tissue quickly and safely, and has high crushing efficiency and a high recovery rate of useful substances.

従来の細胞破砕装置は、例えば公表平２−５０４３６０号公報に示すもの、あるいは図６に示すものがある。これはホモジナイザーとも呼び、高速回転するスクリュウ形状の刃で破砕する方法または回転する内刃と固定された外刃との間で破砕を行う方法である。   Conventional cell crushing devices include, for example, those disclosed in Japanese Published Unexamined Patent Application No. 2-504360 or those shown in FIG. This is also called a homogenizer, and is a method of crushing with a screw-shaped blade rotating at high speed or a method of crushing between a rotating inner blade and a fixed outer blade.

このような細胞破砕装置においては、小さく裁断された柔らかい少量の生体組織片の破砕には適しているが、動物の心臓等の平滑筋を含む組織あるいは骨等の硬い組織に対しては、回転刃のみでは破砕効率が悪く、組織片から蛋白質、ＤＮＡ、ＲＮＡ等を採集できるまでの大きさに破砕にするのに時間かり、また回転内刃と固定外刃との間に裁断され難い平滑筋や硬い組織が挟まり、破砕能力が著しく低下し、処理能力は満足できるものではない。さらに、上記細胞破砕装置に置いては破砕するための回転刃が高速（例えば、毎分２７,０００回転以上）で回転する為、細胞を破砕するときに、回転刃が組織片に高速で何回も当り、回転刃と組織との摩擦により摩擦熱が発生し、細胞から取り出すことを目的とするタンパク質、ＤＮＡ、ＲＮＡ等の熱に弱い物質は変質し、また、回転刃により回収する物質である、タンパク質、ＤＮＡ、ＲＮＡ等を傷め易いので、著しく回収率が低下する。
さらに、上記組織は冷凍保存されることがあり、冷凍されたままの組織を粉砕したいという要望もある。 In such a cell crushing device, it is suitable for crushing a small amount of soft living tissue pieces that are cut into small pieces, but it is not suitable for rotating tissues that contain smooth muscle such as animal hearts or hard tissues such as bones. Smooth muscles that have low crushing efficiency with only the blade, take time to crush to a size that can collect protein, DNA, RNA, etc. from tissue pieces, and are difficult to cut between the rotating inner blade and the fixed outer blade Or a hard structure is pinched, crushing capacity falls remarkably, and processing capacity is not satisfactory. Furthermore, since the rotary blade for crushing in the cell crushing apparatus rotates at a high speed (for example, 27,000 revolutions per minute or more), when the cell is disrupted, the rotary blade does not touch the tissue piece at a high speed. Friction heat is generated due to friction between the rotating blade and the tissue, and heat-sensitive materials such as proteins, DNA, RNA, etc. intended to be taken out from the cells are altered, and substances recovered by the rotating blade Since certain proteins, DNA, RNA and the like are easily damaged, the recovery rate is remarkably reduced.
Furthermore, the tissue may be stored frozen, and there is a desire to grind the tissue that has been frozen.

公表平２−５０４３６０号公報Publication 2-504360

本発明は、上記問題点を改善し、取扱いが簡単で、大量の生態組織を迅速且つ安全に処理できると共に、破砕効率、有用物質の回収率が高い細胞破砕装置を提供することを目的とする。   An object of the present invention is to provide a cell crushing apparatus that improves the above problems, is easy to handle, can process a large amount of ecological tissue quickly and safely, and has high crushing efficiency and a high recovery rate of useful substances. .

供給口を有する収納筒内にモータにより駆動されるスクリュー軸を回転可能に支持し、この収納筒の排出側にはスクリュー軸により送られた生体組織片を破砕する固定刃及び回転刃が取付けられている細胞破砕装置において、前記供給口近傍に生体組織片を凍結保存できる1個または複数の冷却槽を設けることによって達成される。   A screw shaft driven by a motor is rotatably supported in a storage cylinder having a supply port, and a fixed blade and a rotary blade for crushing a biological tissue piece sent by the screw shaft are attached to the discharge side of the storage cylinder. This is achieved by providing one or a plurality of cooling tanks capable of cryopreserving biological tissue pieces in the vicinity of the supply port.

前記冷却槽は冷却液を収納する1個または複数の着脱自在な冷却容器であることで、達成される。   The cooling tank is achieved by being one or a plurality of detachable cooling containers for storing a cooling liquid.

供給口を有する収納筒内にモータにより駆動されるスクリュー軸を回転可能に支持し、この収納筒の排出側にはスクリュー軸により送られた生体組織片を破砕する固定刃及び回転刃が取付けられている細胞破砕装置において、前記供給口及び収納筒に冷却手段を設けることで達成される。   A screw shaft driven by a motor is rotatably supported in a storage cylinder having a supply port, and a fixed blade and a rotary blade for crushing a biological tissue piece sent by the screw shaft are attached to the discharge side of the storage cylinder. This is achieved by providing cooling means in the supply port and the storage cylinder.

前記供給口及び前記収納筒に設けた冷却手段は、冷却液を循環させるための配管であることで達成される。   The cooling means provided in the supply port and the storage cylinder is achieved by being a pipe for circulating the coolant.

前記供給口及び前記収納筒に設けた冷却手段は、冷却液を収納する１個または複数の冷却槽であることで、達成される。   The cooling means provided in the supply port and the storage cylinder is achieved by being one or a plurality of cooling tanks that store the coolant.

前記供給口及び前記収納筒に設けた冷却液を収納する前記冷却層は、１個または複数の着脱自在な冷却容器であることで、達成される。   The cooling layer for storing the coolant provided in the supply port and the storage cylinder is achieved by being one or a plurality of detachable cooling containers.

前記着脱自在な冷却容器のうち、少なくとも１つは破砕前の生体組織片を冷却収納できるような開口部を持つでことで、達成される。   At least one of the detachable cooling containers is achieved by having an opening that can cool and store the biological tissue piece before crushing.

組織片を供給するための供給口を有する収納筒と、該収納筒内に回転可能に支持されたスクリュー軸と、該スクリュー軸を駆動するためのモータと、前記収納筒の排出側にはスクリュー軸により送られた生体組織片を破砕する固定刃及び回転刃とを有した細胞破砕装置による細胞破砕方法において、凍結した組織片または凍結した組織片と氷を混在させた状態で供給することで達成される。   A storage cylinder having a supply port for supplying a tissue piece, a screw shaft rotatably supported in the storage cylinder, a motor for driving the screw shaft, and a screw on the discharge side of the storage cylinder In a cell crushing method using a cell crushing apparatus having a fixed blade and a rotating blade that crush biological tissue pieces sent by a shaft, by supplying frozen tissue pieces or frozen tissue pieces mixed with ice Achieved.

以上、説明したように本発明においては、凍結した生体組織片および骨等を有した生態組織片または、凍結した生体組織片および骨等を有した生態組織片と氷を混在させた状態で細胞破砕装置のモータにより駆動されるスクリュー軸と回転刃により破砕することで、短時間に大量の試料を破砕する事が可能で、且つスクリュー軸の回転が低速（約毎分１０〜１００回転なので、破砕時の刃物と生体組織片との摩擦による発熱は最小限に抑えられる。その結果、取扱いが簡単で、迅速且つ安全に処理できるとともに、破砕効率、有用物質である酵素、ＤＮＡ、ＲＮＡ、タンパク質、抗がん性物質、細胞内微小組織の回収率が高い細胞破砕装置を提供することすることができる。   As described above, in the present invention, in the present invention, a cell in a state where ice is mixed with an ecological tissue piece having frozen biological tissue pieces and bones, or an ecological tissue piece having frozen biological tissue pieces and bones, etc. By crushing with a screw shaft and rotary blade driven by the motor of the crushing device, it is possible to crush a large amount of sample in a short time, and the rotation of the screw shaft is low speed (about 10 to 100 revolutions per minute, Heat generation due to friction between the blade and the tissue piece during crushing is minimized, resulting in easy handling, quick and safe processing, crushing efficiency, and useful substances such as enzymes, DNA, RNA, and proteins In addition, it is possible to provide a cell crushing apparatus having a high recovery rate of anticancer substances and intracellular microtissues.

本発明の一実施例について図面を参照しながら説明する。
本発明の実施例を図１〜図５を用いて説明する。図１は細胞破砕装置の一実施例を示す構造及び部品構成を説明する側面図である。図２は細胞破砕装置の一実施例を示す斜視図である。図３、図４、図５は他の一実施例を示す側面図である。 An embodiment of the present invention will be described with reference to the drawings.
An embodiment of the present invention will be described with reference to FIGS. FIG. 1 is a side view illustrating a structure and a component configuration showing an embodiment of a cell disrupting apparatus. FIG. 2 is a perspective view showing an embodiment of the cell crushing apparatus. 3, 4 and 5 are side views showing another embodiment.

図１において、細胞破砕装置１内には駆動用のモータ２が内蔵されている。破砕部３は細胞破砕装置１に着脱自在に装着され、破砕部３を構成する収納筒７内部にはモータ２の回転軸に着脱自在に嵌合するスクリュー軸４、回転刃５、を回転可能に支持し、さらに、収納筒７の一端には多孔穴６ａを有する固定刃６が固定刃ホルダ２０と図示されていない回転防止部材によって回転できないよう保持されている。さらに、収納筒７には、破砕部３に凍結した生体組織片等を供給するための供給口８が設けられている。   In FIG. 1, a driving motor 2 is built in the cell crushing apparatus 1. The crushing unit 3 is detachably attached to the cell crushing device 1, and a screw shaft 4 and a rotary blade 5 that can be detachably fitted to the rotating shaft of the motor 2 can be rotated inside the storage cylinder 7 constituting the crushing unit 3. Furthermore, the fixed blade 6 having the porous hole 6a is held at one end of the storage cylinder 7 so that it cannot be rotated by the fixed blade holder 20 and a rotation preventing member (not shown). Further, the storage cylinder 7 is provided with a supply port 8 for supplying a frozen living tissue piece or the like to the crushing section 3.

上記構成において、細胞破砕装置１の図示しないスイッチをＯＮすることによりモータ２が駆動して、スクリュー軸４および回転刃５が回転する。
液体窒素等で凍結した生体組織片および骨等を有した生態組織片または、凍結した生体組織片および骨等を有した生態組織片と氷を混在させた状態で供給口８より投入すると、スクリュー軸４に設けられた羽根部４ａによって回転刃５の方向へ押出される。 In the above configuration, the motor 2 is driven by turning on a switch (not shown) of the cell crushing apparatus 1, and the screw shaft 4 and the rotary blade 5 are rotated.
When the biological tissue piece frozen with liquid nitrogen or the like and the biological tissue piece having bones or the like, or the frozen biological tissue piece and the biological tissue piece having bones or the like and ice are mixed and introduced through the supply port 8, the screw Extruded in the direction of the rotary blade 5 by a blade portion 4 a provided on the shaft 4.

スクリュー軸４と収納筒７との隙間は排出口２１側に向うほど徐々に小さくなり、液体窒素等で凍結した生体組織片と氷は圧縮力を受け、細かく砕かれながら回転刃５の方向へ押出され、回転刃５と固定刃６の多孔穴６ａでさらに細かく破砕され、生体組織は凍結したまま、或は、シャーベット状の状態で、排出口２１から破砕部３外に排出される。   The gap between the screw shaft 4 and the storage cylinder 7 gradually decreases toward the discharge port 21, and the biological tissue piece and ice frozen with liquid nitrogen or the like are subjected to compressive force and are finely crushed toward the rotary blade 5. Extruded and further finely crushed in the perforated holes 6a of the rotary blade 5 and the fixed blade 6, and the living tissue is discharged out of the crushing section 3 from the discharge port 21 in a frozen state or in a sherbet-like state.

このような構成とすることで、凍結した生体組織片は従来の細胞破砕装置と比較して、ごく短時間に粉砕されるので破砕時の熱による影響は最小限に抑えられ、かつ回転式ホモジナイザーと比較して生体組織片が高速で回転する回転刃によって傷つけられることもなく、また、摩擦熱によるタンパク質、ＤＮＡ、ＲＮＡ等の熱に弱い物質の変質による回収率の低下や分子量の大きいタンパク等の抽出のような場合おいても、タンパク質等の回収物を傷つけることが少なくなり、生体組織抽出率の飛躍的な上昇につながった。例えば人肝臓からのミクロゾーム抽出に用いた場合、このミクロゾームが薬物を代謝する能力は、従来法で抽出したものに比べ数％向上する結果を得られた。   By adopting such a configuration, frozen biological tissue fragments are pulverized in a very short time compared to conventional cell crushing devices, so that the influence of heat during crushing can be minimized, and a rotary homogenizer Compared to the above, biological tissue fragments are not damaged by a rotating blade that rotates at high speed, and the recovery rate is reduced due to alteration of heat-sensitive materials such as proteins, DNA, RNA, etc. due to frictional heat, proteins with large molecular weight, etc. Even in the case of extraction, the recovery of proteins and the like is less likely to be damaged, leading to a dramatic increase in the extraction rate of living tissue. For example, when microsomes were extracted from human liver, the ability of these microsomes to metabolize drugs was improved by several percent compared to those extracted by conventional methods.

さらに従来の細胞破砕装置では、連続的に生体組織片投入し、破砕することはできなかったが、本発明では連続的に生体組織片等の試料を供給口８より投入することも可能で、従来の細胞破砕機と比較し大幅な処理量の増加が達成でき、製薬企業等の工業的利用が可能となった。   Furthermore, in the conventional cell crushing apparatus, it was not possible to continuously put and crush biological tissue pieces, but in the present invention, it is also possible to continuously feed samples such as biological tissue pieces from the supply port 8, Compared to conventional cell disrupters, a significant increase in throughput was achieved, enabling industrial use by pharmaceutical companies and the like.

また、凍結した生体組織片と氷を混在させた状態で供給口８から投入した場合は、氷が破砕助剤となり生体組織片単体で破砕するよりも破砕効率が向上し、さらに破砕すべき生体組織がなくなった後、氷のみを供給口８から投入することにより、破砕部３に残留していた生体組織も氷に押し出され、無駄のない高い回収率で破砕することができる。なおかつ、生体組織内の生体成分を温度の変化や酸化から守るために緩衝液の凍結氷を用いることで、タンパク質、ＤＮＡ、ＲＮＡ等が水に触れることによる変性を防止する効果の向上を図ることができた。   In addition, when the frozen biological tissue piece and ice are mixed and introduced from the supply port 8, the crushing efficiency is improved compared to the case where ice becomes a crushing aid and the biological tissue piece alone is crushed, and further the living body to be crushed. By supplying only ice from the supply port 8 after the tissue disappears, the living tissue remaining in the crushing unit 3 is also pushed out to the ice, and can be crushed with a high recovery rate without waste. In addition, the use of frozen ice in the buffer solution to protect the biological components in the living tissue from temperature changes and oxidation improves the effect of preventing denaturation due to the contact of proteins, DNA, RNA, etc. with water. I was able to.

さらに図１において処理前の生体組織片等の試料を液体窒素等の冷却液で瞬時凍結する為の凍結槽９を供給口８近傍に設置した。破砕する試料は凍結槽内の液体窒素など瞬時凍結し、凍結槽９から直ちに供給口８に挿入、破砕する事ができるため、解凍することなく瞬時に破砕する事が可能となりタンパク質、ＤＮＡ、ＲＮＡ等の変性を防止する大きな効果が得られる。   Further, in FIG. 1, a freezing tank 9 for instantly freezing a sample such as a biological tissue piece before processing with a cooling liquid such as liquid nitrogen is installed in the vicinity of the supply port 8. The sample to be crushed can be instantly frozen, such as liquid nitrogen in the freezing tank, and immediately inserted into the supply port 8 from the freezing tank 9 and crushed. A great effect of preventing such denaturation is obtained.

また図２に示す実施例では凍結槽は本体から着脱自在な冷却容器１０とする事で、装置から離れた場所に試料ごと保管することも可能となり、かつ破砕後の凍結槽の洗浄も冷却容器１０を取り外すことで容易にすることができる。   In the embodiment shown in FIG. 2, the freezing tank is a cooling container 10 that is detachable from the main body, so that the sample can be stored in a place away from the apparatus, and the freezing tank after crushing can be washed as a cooling container. This can be facilitated by removing 10.

さらに図３では細胞破砕装置１の供給口８及び収納筒７に冷却機構１１を設け、破砕時の熱を安定的に抑える事を工夫した。これにより大量の試料を処理する場合でも熱による回収率低下が無くなり、生体組織抽出率の安定的な維持が可能となった。
本実施例では冷却機構１１として冷却液を流す冷却パイプを供給口８及び収納筒７に巻きつけた構造とし、冷却機構１１の入り口側冷却パイプ１１ａからフロン等の冷媒を流し、出口側冷却パイプ１１ｂより回収する。冷却機構１１外周には発泡ウレタン等の断熱材１１ｃを設置する事で冷却効果は向上する。またモータ２からの熱の流入を防ぐため、熱伝導度の低いプラスチック製のカップリング２５を介してモータ２の軸とスクリュー軸４を結合する事でさらに冷却効果を向上することができる。 Furthermore, in FIG. 3, the cooling mechanism 11 was provided in the supply port 8 and the storage cylinder 7 of the cell crushing apparatus 1, and it devised to suppress the heat | fever at the time of crushing stably. As a result, even when a large amount of sample is processed, there is no decrease in the recovery rate due to heat, and it is possible to stably maintain the biological tissue extraction rate.
In this embodiment, the cooling mechanism 11 has a cooling pipe for flowing a cooling liquid around the supply port 8 and the storage cylinder 7, and a refrigerant such as chlorofluorocarbon is flowed from the inlet side cooling pipe 11 a of the cooling mechanism 11, and the outlet side cooling pipe It collects from 11b. The cooling effect is improved by installing a heat insulating material 11c such as urethane foam on the outer periphery of the cooling mechanism 11. Further, in order to prevent heat from flowing from the motor 2, the cooling effect can be further improved by coupling the shaft of the motor 2 and the screw shaft 4 via a plastic coupling 25 having low thermal conductivity.

図４に示す細胞破砕装置１では冷却機構１１を冷却液１３を収納する1個または複数の冷却槽１２とした。冷却槽１２の中に直接液体窒素等の冷却液を満たしておく事で冷凍機等の大掛かりな装置を必要とせずに、破砕時の熱を安定的に抑えることが可能となった。   In the cell disruption apparatus 1 shown in FIG. 4, the cooling mechanism 11 is one or a plurality of cooling tanks 12 that store the cooling liquid 13. By directly filling the cooling tank 12 with a cooling liquid such as liquid nitrogen, it is possible to stably suppress heat during crushing without requiring a large-scale device such as a refrigerator.

図５に示す実施例では冷却機構１１を冷却液１３を入れる1個または複数の着脱自在な冷却容器１４とした。これにより液体窒素等の冷却液を補給するときには冷却容器のみを持ち運び、補給すればよく、破砕作業がより簡単、迅速、安全となった。
冷却容器１４と破砕部３との接触部には凹凸を付け表面積を広げて冷却効率を良くした。また接触部には熱伝導グリース等を塗布することにより、冷却効率の向上を図ることができる。 In the embodiment shown in FIG. 5, the cooling mechanism 11 is one or a plurality of detachable cooling containers 14 into which the cooling liquid 13 is put. As a result, when replenishing a cooling liquid such as liquid nitrogen, it is only necessary to carry and replenish the cooling container, making the crushing operation easier, quicker and safer.
The contact portion between the cooling container 14 and the crushing portion 3 is provided with unevenness to increase the surface area, thereby improving the cooling efficiency. In addition, it is possible to improve the cooling efficiency by applying thermal conductive grease or the like to the contact portion.

さらに冷却容器１４の一つは大きな開口部を持つ冷却容器１５とする事で、前記した生体組織片等の試料を液体窒素等の冷却液で瞬時凍結する為の凍結槽としても兼ねることができ、より効率の良い破砕作業が実現できた。   Furthermore, one of the cooling containers 14 is a cooling container 15 having a large opening, so that it can also serve as a freezing tank for instantly freezing a sample such as a biological tissue piece with a cooling liquid such as liquid nitrogen. A more efficient crushing operation was realized.

本発明の一実施例を示す細胞破砕装置の構造及び部品構成を説明する側面図である。It is a side view explaining the structure and component structure of a cell crushing apparatus which shows one Example of this invention. 本発明の一実施例を示す細胞破砕装置の斜視図である。It is a perspective view of the cell crushing apparatus which shows one Example of this invention. 本発明の一実施例による細胞破砕装置の側面図である。It is a side view of the cell crushing apparatus by one Example of this invention. 本発明の一実施例による細胞破砕装置の側面図である。It is a side view of the cell crushing apparatus by one Example of this invention. 本発明の一実施例による細胞破砕装置の側面図である。It is a side view of the cell crushing apparatus by one Example of this invention. 本従来の細胞破砕装置の断面図である。It is sectional drawing of this conventional cell crushing apparatus.

符号の説明Explanation of symbols

１ 細胞破砕装置本体１、２ モータ、３ 破砕部、４ スクリュー軸、５ 回転刃、６ 固定刃、６ａ 多孔穴、７ 収納筒、８ 供給口、９ 冷却槽、１０冷却容器、１１ 冷却機構、１１ａ入り口側冷却パイプ、１１ｂ 出口側冷却パイプ、１２ 冷却槽、１３ 冷却液、１４ 冷却容器、１５ 開口部を持つ冷却容器
DESCRIPTION OF SYMBOLS 1 Cell crusher main body 1, 2 Motor, 3 Crushing part, 4 Screw shaft, 5 Rotary blade, 6 Fixed blade, 6a Porous hole, 7 Storage cylinder, 8 Supply port, 9 Cooling tank, 10 cooling container, 11 Cooling mechanism, 11a Inlet side cooling pipe, 11b Outlet side cooling pipe, 12 Cooling tank, 13 Coolant, 14 Cooling container, 15 Cooling container having an opening

Claims (8)

供給口を有する収納筒内にモータにより駆動されるスクリュー軸を回転可能に支持し、この収納筒の排出側にはスクリュー軸により送られた生体組織片を破砕する固定刃及び回転刃が取付けられている細胞破砕装置において、
前記供給口近傍に生体組織片を凍結保存できる1個または複数の冷却槽を設けた事を特徴とする細胞破砕装置。 A screw shaft driven by a motor is rotatably supported in a storage cylinder having a supply port, and a fixed blade and a rotary blade for crushing a biological tissue piece sent by the screw shaft are attached to the discharge side of the storage cylinder. Cell disruption equipment
A cell disruption apparatus characterized in that one or more cooling tanks capable of cryopreserving biological tissue fragments are provided in the vicinity of the supply port. 前記冷却槽は冷却液を収納する1個または複数の着脱自在な冷却容器である事を特徴とする請求項２記載の細胞破砕装置。 The cell disruption apparatus according to claim 2, wherein the cooling tank is one or a plurality of detachable cooling containers for storing a cooling liquid. 供給口を有する収納筒内にモータにより駆動されるスクリュー軸を回転可能に支持し、この収納筒の排出側にはスクリュー軸により送られた生体組織片を破砕する固定刃及び回転刃が取付けられている細胞破砕装置において、
前記供給口及び収納筒に冷却手段を設けた事を特徴とする細胞破砕装置。 A screw shaft driven by a motor is rotatably supported in a storage cylinder having a supply port, and a fixed blade and a rotary blade for crushing a biological tissue piece sent by the screw shaft are attached to the discharge side of the storage cylinder. Cell disruption equipment
A cell disruption apparatus characterized in that a cooling means is provided in the supply port and the storage cylinder. 前記供給口及び前記収納筒に設けた冷却手段は、冷却液を循環させるための配管であることを特徴とする請求項３記載の細胞破砕装置。 The cell disruption apparatus according to claim 3, wherein the cooling means provided in the supply port and the storage cylinder is a pipe for circulating a coolant. 前記供給口及び前記収納筒に設けた冷却手段は、冷却液を収納する1個または複数の冷却槽である事を特徴とする請求項３記載の細胞破砕装置。 4. The cell disruption apparatus according to claim 3, wherein the cooling means provided in the supply port and the storage cylinder is one or a plurality of cooling tanks that store a coolant. 前記供給口及び前記収納筒に設けた冷却液を収納する前記冷却層は、１個または複数の着脱自在な冷却容器である事を特徴とする請求項５記載の細胞破砕装置。 6. The cell disruption apparatus according to claim 5, wherein the cooling layer storing the coolant provided in the supply port and the storage cylinder is one or a plurality of detachable cooling containers. 前記着脱自在な冷却容器のうち、少なくとも１つは破砕前の生体組織片を冷却収納できるような開口部を持つ事を特徴とする請求項６記載の細胞破砕装置。 7. The cell disruption apparatus according to claim 6, wherein at least one of the detachable cooling containers has an opening that can cool and store the biological tissue piece before being disrupted. 組織片を供給するための供給口を有する収納筒と、
該収納筒内に回転可能に支持されたスクリュー軸と、
該スクリュー軸を駆動するためのモータと、
前記収納筒の排出側にはスクリュー軸により送られた生体組織片を破砕する固定刃及び回転刃とを有した細胞破砕装置による細胞破砕方法において、
凍結した組織片または凍結した組織片と氷を混在させた状態で供給することを特徴とする細胞破砕方法。
A storage cylinder having a supply port for supplying tissue pieces;
A screw shaft rotatably supported in the storage cylinder;
A motor for driving the screw shaft;
In the cell crushing method by the cell crushing device having a fixed blade and a rotating blade for crushing the biological tissue piece sent by the screw shaft on the discharge side of the storage cylinder,
A cell disruption method comprising supplying a frozen tissue piece or a frozen tissue piece mixed with ice.
JP2003368172A 2003-10-28 2003-10-28 Cell disrupter and, method for disrupting cell Withdrawn JP2005130730A (en)

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JP2007222811A (en) * 2006-02-24 2007-09-06 Higashi Nippon Gakuen Hokkaido Iryo Daigaku Evulsed tooth crushing article and evulsed tooth-originating delimed powder applicable to high-tech medical treatment, and method and crusher for preparing complex of delimed powder and apatite
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007222811A (en) * 2006-02-24 2007-09-06 Higashi Nippon Gakuen Hokkaido Iryo Daigaku Evulsed tooth crushing article and evulsed tooth-originating delimed powder applicable to high-tech medical treatment, and method and crusher for preparing complex of delimed powder and apatite
WO2007099861A1 (en) * 2006-02-24 2007-09-07 Health Sciences University Of Hokkaido Milled product of extracted tooth usable in highly advanced medical treatment, decalcified powder originating in extracted tooth, method of preparing composite of decalcified powder with apatite and milling machine
US8752777B2 (en) 2006-02-24 2014-06-17 Health Sciences University Of Hokkaido Method and pulverizing apparatus for preparing pulverized product of extracted tooth, demineralized powder originated from extracted tooth, and composite of demineralized powder and apatite, suitable for use in highly advanced medical treatments
CN108187854A (en) * 2018-01-12 2018-06-22 义乌市晶凯机械设备有限公司 A kind of biological medicine milling apparatus
CN108212406A (en) * 2018-01-30 2018-06-29 陈汝杏 A kind of modified clinical waste collecting and transport device
WO2020004698A1 (en) * 2018-06-27 2020-01-02 (주) 티아라줄기세포연구소 Preparation method for cell culture medium composition, preparation method for stem cell lysate extract (shelled stem cell) using preparation method for cell culture medium composition and three-conditional extraction method of effective ingredient from stem cell, anti-arthritis therapeutic composition using same, anti-inflammatory therapeutic composition using same, and cell-regenerating therapeutic composition
KR20200001370A (en) * 2018-06-27 2020-01-06 주식회사 티아라줄기세포연구소 Crushed Stem Cell Extract(Shelled Stem Cell) Manufacturing Method Using Mass Culture Medium Composition Method and Constituent 3-low Extracting Method and A Treating Composition for Anti-Inflammatory and A Treating Composition for Cell Regeneration
KR102121602B1 (en) * 2018-06-27 2020-06-10 주식회사 티아라줄기세포연구소 Crushed Stem Cell Extract(Shelled Stem Cell) Manufacturing Method Using Mass Culture Medium Composition Method and Constituent 3-low Extracting Method and A Treating Composition for Anti-Inflammatory and A Treating Composition for Cell Regeneration
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