JP2003292417A - Epidermal cell-activating composition, enderonic fibroblast-activating composition, collagen production- promoting composition, and skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an epidermal cell-activating composition, enderonic fibroblast-activating composition or collagen production-promoting composition which activates epidermal cells to normalize their function and put their turnover in order, activates enderonic fibroblast to raise collagen production, thus keeps the skin healthy and is effective for preventing or ameliorating skin aging symptoms such as wrinkles and skin resilience decline due to various stresses including aging and ultraviolet radiation, and to provide a skin care preparation containing one or more of the compositions. <P>SOLUTION: The epidermal cell-activating composition, enderonic fibroblast- activating composition or collagen production-promoting composition is obtained by including the extract of a plant (preferably, water extract) belonging to the genus Clintonia, Uvulariaceae family, either directly or by including the extract in a carrier or external preparation base. The skin care preparation is obtained by including one or more of said compositions. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to activating epidermal cells to normalize their functions and arrange turnover, and to activate dermal fibroblasts to enhance collagen production to keep skin healthy. A composition for promoting epidermal cells, a composition for promoting dermal fibroblasts, a composition for promoting collagen production, which is effective in preventing or ameliorating skin aging symptoms such as wrinkles due to various stresses such as aging and ultraviolet rays and reduction in skin elasticity. And an external preparation for skin. For more details,
Ubulariaacea ( Uvulariaceae ) Family Swallow Omoto ( Clin
tonia ) plant extract, preferably a composition for promoting epidermal cells, a composition for promoting dermal fibroblasts, a composition for promoting collagen production, and a skin external preparation containing an extract of water.

[0002]

2. Description of the Related Art Conventionally, due to aging, exposure to ultraviolet rays, etc., the stratum corneum of the skin, epidermal cells and dermal cells are damaged, the number of cells in the epidermis is reduced, the metabolism of epidermal cells is reduced, and the skin turns. It is known that the overspeed becomes slow, the hyaluronic acid is reduced in the dermis, and collagen and elastin are denatured, causing aging of the skin such as wrinkle formation and deterioration of elasticity and rough skin symptoms. In order to prevent or improve these processes, many skin external preparations have been proposed. Recently, reflecting the natural and plant orientation, in plant extract components, there are active radicals such as hydroxy radicals, singlet oxygen, superoxide, and other active oxygen species;
Actively search for ingredients that suppress the degradation of collagen, elastin, hyaluronic acid, etc., which are the constituents of the dermal matrix, and that promote their production, and that activate epidermal cells and dermal fibroblasts. ing.

As the above-mentioned antioxidant component, various plant extracts are used, including plant extracts containing tannin, flavonoids, polyphenol compounds and the like. As a component having an action of inhibiting collagenase activity for degrading collagen, an extract of pomegranate, lemon balm, etc. (JP-A-7-196526), an extract of Pleurotus cornucopiae (JP-A-11-79970), etc., promotes collagen production. As an extract, an extract of a plant of the genus Elephantpath (Asteraceae) (Japanese Patent Laid-Open No. 9-87135), a shoot of a beech plant of the Beech family (Japanese Patent Laid-Open No. 10-203952), and the like are disclosed. Examples of components that inhibit the elastase activity that decomposes elastin include birch, keihi, fubodaiju, natsubodaiju, linden and the like (JP-A-11-171).
758), Bergenia classifolia, etc.
1-199504), and examples of components that inhibit hyaluronidase activity that decomposes hyaluronic acid include clove, areca and the like (JP-A-6-9371),
Chinpi, Kinjitsu et al. (JP-A-6-80576), sumacaceous plant (JP-A-7-10765), leguminous plant (JP-A-7-10768), arnica, dodami, etc.
-130162) and the like, as a component that promotes the production of hyaluronic acid, seaweed Anahaosa (JP-A-6-942).
In addition to 2), Mesooi (JP-A-9-87163), Lamiaceae plants (JP-A-10-95735), Moraceae plants (JP-A-11-60496) and the like are disclosed. In addition, as a component that activates dermal fibroblasts of the skin, almond, dandelion, elderberry, senkyu, senburi, sokuhaku, tonin, carrot, hop, mukuge, yokuinin (JP-A-10-36279) and the like, epidermal cells As a component for activating coconut, a combination of parsley extract and beech tree extract (JP-A-11-335257)
Etc. are disclosed.

However, among the plant extract components that have been used conventionally, the effects of preventing and improving the symptoms of skin aging and rough skin are not sufficient, and when incorporated in a skin external preparation,
There have been some that must be used in large amounts or that must be used in combination with other components having anti-aging or anti-inflammatory effects. In addition, there were some which could not obtain an extract having stable and constant quality.

[0005]

Therefore, in the present invention, a component having an action of activating epidermal cells and dermal fibroblasts and promoting collagen production is searched for, and more effective prevention of skin aging and rough skin symptoms, The object was to obtain a composition for promoting epidermal cells, a composition for promoting dermal fibroblasts, a composition for promoting collagen production, and an external preparation for skin which have an improving effect.

[0006]

[Means for Solving the Problems] In order to solve the above-mentioned problems, when a component that activates epidermal cells and dermal fibroblasts without showing cytotoxicity and promotes collagen production was screened, Ubularia cere ( Uvulariace
It was found that an extract of the genus Clintonia of the family ae ) has a high activity of activating epidermal cells, activating dermal fibroblasts, and promoting collagen production. Furthermore, they have found that the application of these to the skin can effectively prevent and improve the skin aging and rough skin symptoms, and have completed the present invention.

That is, in the present invention, the extract of the plant of the genus Clintonia of the family Ubulariaceae ( Uvulariaceae ) is used as it is, or is contained in a carrier or a base, and a composition for utilizing epidermal cells, a composition for utilizing dermal fibroblasts, A composition for promoting collagen production. Alternatively, a skin external preparation is prepared by incorporating these into an external preparation base.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The composition for promoting epidermal cells, the composition for promoting dermal fibroblasts, the composition for promoting collagen production, and the composition for promoting skin production of the present invention, which is used for obtaining an external preparation for skin, is a vulbereaceae family, a swallowfish moto ( Uvulariaceae ). Clintonia )
The genus plant is sometimes classified into the Liliaceae family and is a perennial plant that is isolated and distributed in North America and East Asia.
Clint near-Andre cattle Ana (C. Andrewsiana),
Clint near Borealis (C. Borealis), Clint near-Udenshisu (C. Udensis), Clint near-Unberurata (C. Umbellulata), Clint near-Uni Flora (C. Uniflora) is known, easy to obtain From this point, Clintonia borealis ( C. borealis ) and Clintonia udensis ( C. udensis ) can be preferably used. For extraction, one or more species selected from each part of flowers, fruits, stems, leaves, roots, etc., or whole grass is used.

Although these may be subjected to the extraction operation as they are, it is preferable to carry out the extraction after the processing such as shredding, drying and crushing is taken into consideration in consideration of the extraction efficiency. The extraction is performed by immersing it in an extraction solvent. Stirring to improve extraction efficiency,
You may homogenize in an extraction solvent. As the extraction temperature, it is appropriate to set the temperature to about 5 ° C. to a temperature not higher than the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is suitable to be about 4 hours to 14 days.

As the extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, diethyl ether and dipropyl. Polar organic solvents such as ethers such as ethers, esters such as ethyl acetate and butyl acetate, ketones such as acetone and ethyl methyl ketone are preferably used, and one or more kinds thereof are selected and used. Alternatively, physiological saline, phosphate buffer, phosphate buffered saline, etc. may be used.

The extraction solvent is not particularly limited as long as it is the above solvent, but a polar solvent is preferable, and water is most preferably used.

The extract of the plant of the genus Swallowweed ( Clintonia ) of the family Uvulariaceae belonging to the above-mentioned solvent is used as it is as a composition for promoting epidermal cells, a composition for promoting dermal fibroblasts, and a composition for promoting collagen production. However, it can be used after reconstitution of concentrated and dried solids in water or polar solvent, or after purification or fractionation such as decolorization, deodorization, desalting, etc. within the range that does not impair its action. Good. For storage, it is preferable to freeze-dry after purification and dissolve in a solvent before use. It can also be encapsulated in vesicles such as liposomes or microcapsules.

In the present invention, the extract of the plant of the genus Clintonia of the family Uvulariaceae or the treated product is used as it is, or a base such as an aqueous carrier such as water or alcohol, an emulsion, a gel, a cream, an ointment or the like. To give a composition for promoting epidermal cells, a composition for promoting dermal fibroblasts, and a composition for promoting collagen production. Alternatively, these are contained in an external preparation base to provide an epidermal cell stimulating composition, a dermal fibroblast stimulating composition, a collagen production promoting composition, and a skin external preparation.

The external preparation for skin according to the present invention can be provided in the form of lotion, emulsion, gel, cream, ointment and the like. In addition, skin cosmetics such as lotions, emulsions, creams and packs, makeup base lotions, makeup base creams, emulsions or cream-like or ointment type foundations, makeup cosmetics such as eye colors and cheek colors, hand products. It can also be provided as body cosmetics such as creams, leg creams and body lotions, cleaning agents such as body shampoos and soaps, hair cosmetics such as treatments and setting agents.

The epidermal cell utilization composition according to the present invention,
In addition to the extract according to the present invention, oils, surfactants, humectants, ultraviolet absorbers, pigments, dermal fibroblast stimulating compositions, collagen production promoting compositions, and skin external preparations can be used.
General raw materials for pharmaceuticals and cosmetics such as fragrances and preservatives,
Physiologically active ingredients such as active oxygen scavengers, anti-inflammatory agents, and whitening agents can also be included. The amount of the extract according to the present invention to be added to the external preparation for skin varies depending on the dosage form, but is preferably about 0.0001 to 10.0% by weight.

[0016]

The present invention will be described in more detail with reference to Examples.

Swallow's moto plant extract 1 After drying and crushing the whole plant of Clintonia borealis,
500 g was immersed in 1000 mL of purified water and allowed to stand at 25 ° C. for 7 days. Then, the insoluble matter was removed by filtration to obtain a swallow moto plant extract 1.

Swallow's moto plant extract 2 Clintonia uddensis was dried and crushed, and then 500
g was immersed in 1000 mL of purified water and allowed to stand at 25 ° C. for 7 days. After that, the insoluble matter was removed by filtration to obtain a genus Plant extract 2 of the genus Swallow.

Swallow moto plant extract 3 The total amount of the swallow moto extract 1 prepared above is 100.
Concentrate under reduced pressure to reach mL. Soy lecithin 80
g was added and suspended at 65 ° C., and then treated with ultrasonic waves to prepare liposomes, which were collected by centrifugation to give an extract 3 of the genus Swallowweed.

Swallow's moto genus extract 4 The solvent of the swallow's moto genus extract 2 prepared above is
After concentration under reduced pressure and removal, the mixture is dried. This was added to 500 mL of 1,3-butylene glycol and dissolved therein to obtain a swallow moto plant extract 4.

Epidermal Cell Activating Effect The extract of the plant of the genus Swallowtail motogenus 1 of the present invention was evaluated for epidermal cell activating effect. The evaluation method was as follows: Normal human epidermal keratinocytes were seeded in a 96-well microplate so that the number of cells per well was 2.0 × 10 ⁴ , and the swallow moto plant extract 1 was tested at 40 μg / mL to 5000 μg / mL in each test. Medium for growing epidermal keratinocytes (HuMedia
a-KG2) at 37 ° C. for 24 hours, then 2-
100 μg / m of (4,5-dimethyl-2-thiazolyl) -3,5-diphenyltetrazolium bromide (MTT)
1-containing medium and incubated at 37 ° C. for 2 hours to give 550 formazan produced by ring opening of the tetrazolium ring.
A method of quantifying by absorbance at nm was used. In addition, the system cultivated without addition of the swallowwort genus plant extract 1 was used as a control. The results are shown in Table 1 by the activation index in which the absorbance in the control is 100.

[0022]

[Table 1]

[0023] As shown in Table 1, the swallow moto plant extract 1 according to the present invention contains 5000 μg / mL.
Epidermal keratinocytes were significantly activated at this concentration. In addition,
No cytotoxicity was observed in the concentration range tested.

Dermal Fibroblast Activating Action The dermal fibroblast activating action of the swallowtail motophyte extract 1 of the present invention was evaluated. As an evaluation method, normal human fibroblasts were seeded on a 96-well microplate so that the number of cells per well was 2.0 × 10 ⁴ , and the swallow moto plant extract 1 was 7.82 μg / mL to 1000 μg / m 2.
Cultured in Dulbecco's modified basal medium (DMEM) containing 1.0% by volume of fetal bovine serum containing L of each test concentration for 2 days at 37 ° C., and then 2- (4,5-dimethyl-2-thiazolyl) -3, 5-diphenyltetrazolium bromide (MT
T) was replaced with a medium containing 400 μg / ml, and the temperature was 37 ° C.
After culturing for 2 hours at room temperature, the method of quantifying the formazan generated by the opening of the tetrazolium ring by the absorbance at 550 to 650 nm was used. It should be noted that DMEM containing 1.0% by volume of fetal bovine serum was added without the addition of the extract of the genus Swallowweed.
The system cultivated by using as a control was used as a control, and the system culturing by DMEM supplemented with 5.0 volume% fetal bovine serum was used as a positive control. The results are shown in Table 2 by the activation index with the absorbance in the control being 100.

[0025]

[Table 2]

As shown in Table 2, the swallow moto plant extract 1 according to the present invention significantly activates dermal fibroblasts in the tested concentration range, which is a very dilute test concentration. At 82 μg / mL, it is the same level as the positive control, and further 31.25 μg / mL to 500 μg / mL.
In the concentration range of mL, the value was higher than that of the positive control. No cytotoxicity was observed in the tested concentration range.

Collagen production accelerating effect The effect of the swallow moto plant extract 1 of the present invention on the collagen producing ability of normal human dermal fibroblasts was examined. That is, normal human dermal fibroblasts were seeded on a 96-well microplate so that the number of cells per well was 2.0 × 10 ⁴ , and 7.8% of the swallow moto plant extract 1 was seeded.
After culture for 5 days in Dulbecco's modified basal medium (DMEM) containing 0.5% by volume of fetal bovine serum containing each test concentration of 3 μg / mL to 250 μg / mL, the amount of collagen in the culture supernatant was assayed by enzyme-linked immunosorbent assay. (Enzyme-linked
Immunosorbent assay (ELISA) was used, and at the same time, the number of fibroblasts was counted and the amount of collagen produced per cell was calculated. It should be noted that 0.5% by volume of fetal calf serum was added without addition of the swallow moto plant extract 1D
A system cultured in MEM alone was used as a control, and a system cultured in DMEM supplemented with 50 μM magnesium ascorbyl phosphate-0.5 vol% fetal bovine serum was used as a positive control. The results are shown in Table 3 by the activation index in which the amount of collagen produced in the control was 100.

[0028]

[Table 3]

As shown in Table 3, the swallow moto plant extract 1 according to the present invention was 62.5 μg / mL.
Collagen production was significantly promoted in the concentration range of ˜250 μg / mL. No cytotoxicity was observed in the tested concentration range.

Next, the formulation of the examples of the external preparation for skin according to the present invention will be shown. Further, unless otherwise specified, the unit weight is shown in% by weight.

[Example 1] Lotion for skin (1) Ethanol 10.0 (2) Hydroxyethyl cellulose 1.0 (3) Swallow's moto plant extract 1 0.5 (4) Swallow's moto plant extract 20. 5 (5) Methyl paraoxybenzoate 0.1 (6) Purified water Amount with 100 as the total amount Production method: (1) to (5) are sequentially added to (6) and uniformly dissolved.

Example 2 Skin Emulsion (1) Stearic Acid 0.20 (2) Cetanol 1.50 (3) Vaseline 3.00 (4) Liquid Paraffin 7.00 (5) Polyoxyethylene (10E. O.) Monooleate 1.50 (6) Tocopherol acetate 0.50 (7) Glycerin 5.00 (8) Methyl paraoxybenzoate 0.10 (9) Potassium hydroxide 0.02 (10) Purified water Total amount (11) Swallow's moto plant extract 1 0.50 (12) Swallow's moto plant extract 2 0.50 Preparation method: Mix the oil phase components of (1) to (6) and heat to homogeneity. Melt to 70 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and heated to homogenize the mixture to 70 ° C. The oil phase component was gradually added to this water phase component while stirring to emulsify,
After cooling, (11) and (12) are added and mixed at 40 ° C.

[Example 3] Liposomal agent for skin (1) Glycerin 2.0 (2) 1,3-butylene glycol 3.0 (3) Polyoxyethylene (25 EO) oleyl ether 0.2 (4 ) Ethanol 10.0 (5) Methyl paraoxybenzoate 0.1 (6) Perfume 0.1 (7) Purified water Amount based on 100 as the total amount (8) Swallow moto plant extract 3 10.0 Production method: (5) , (6) is dissolved in (4), along with (1)-(3)
Add to (7) and mix uniformly, then add (8) to this and disperse.

Example 4 Skin Cream (1) Beeswax 6.0 (2) Cetanol 5.0 (3) Reduced Lanolin 8.0 (4) Squalane 27.5 (5) Glyceryl Fatty Acid Ester 4.0 ( 6) Lipophilic glyceryl monostearic acid ester 2.0 (7) Polyoxyethylene (10 EO) sorbitan monolauric acid ester 5.0 (8) Propylene glycol 5.0 (9) Swallow moto plant extract 4 3 0.0 (10) Methyl paraoxybenzoate 0.1 (11) Purified water Amount with 100 as the total amount Production method: Mix and dissolve the oil phase components of (1) to (7) to 75 ° C. On the other hand, the water phase components (8) to (11) are mixed, dissolved and heated to 75 ° C. Next, the oil phase component is added to this aqueous phase component to pre-emulsify it, then homogenize with a homomixer and cool.

Next, for Examples 1 to 4 of the present invention, an actual use test for 6 months was conducted. At this time, an extract 1 of the genus Plant of the genus Swallowtail and an extract 2 of the plant of the genus Swallowtail moto
Was prepared by substituting the purified water for the swallow-tailed moto plant extract 3 in the liposome prepared in the same manner without adding the plant extract, and replaced the swallow-tailed moto plant extract 4 with 1,3-butylene glycol. An actual use test was simultaneously performed for Examples 1 to 4.

In a practical use test for evaluation of aging symptom improvement, a group of 20 females in their 40s to 60s who exhibited remarkable skin aging symptoms such as wrinkles and decreased skin elasticity were used as panelists for each group. The examples and the comparative examples were each made to be used in the blind twice a day. The skin condition was observed before and after the start of the use test, and the improvement status of wrinkles and skin elasticity was evaluated in three grades of "improved", "slightly improved", and "no change". The degree of wrinkles was evaluated by photographing and replica sampling, and the elasticity of the skin was evaluated by measuring with a cute meter. The results are shown in Table 4 by the number of panelists who obtained each evaluation.

[0037]

[Table 4]

As is clear from Table 4, in all of the groups used in the examples of the present invention, both panel wrinkles and skin elasticity showed a tendency of improvement in symptoms, and wrinkles 5 were observed.
0% or more, and 70% or more of the panelists showed a clear improvement in skin elasticity. On the other hand, although good wrinkles and improvement in skin elasticity may be observed in the comparative use group, the extent thereof is smaller than that of the corresponding use group.

In the actual use test for the evaluation of the improvement of the rough skin condition, as a panelist, 20 women each having a remarkable rough skin condition were used as a group, and each of the examples and the comparative examples were blinded twice a day. I used it. The state of the skin was observed before and after the start of the use test, and the improvement status of the rough skin condition was evaluated in three stages of "improvement", "slight improvement", and "no change". The results are shown in Table 5 by the number of panelists who obtained each evaluation.

[0040]

[Table 5]

As is clear from Table 5, in any of the groups used in the examples of the present invention, remarkable improvement in rough skin was observed, and the skin condition was improved to a substantially good condition after the end of the use test. . On the other hand, in the case of using the comparative example, although the improvement of the rough skin may be recognized in some cases, the extent of the improvement was smaller than that of the corresponding group using the example.

Next, 20 panelists having skin symptoms such as hyperproliferation and parakeratosis of keratin were used as one group, and the above-mentioned examples and comparative examples were blinded to each group.
The state of keratinocytes collected by the tape stripping method was observed before and after use by applying 1 g twice a day to the affected area for one month. The results were scored according to the criteria in Table 6 and the average value of 20 persons was calculated and shown in Table 7.

[0043]

[Table 6]

[0044]

[Table 7]

As is clear from Table 7, the morphology of keratinocytes collected before use was poor in most panelists, the abundance of nucleated cells was high, and multiple exfoliation was frequently observed. In each of the groups used in Examples of the invention, recovery of cell morphology was observed after use, and significant decrease in the number of nucleated cells and the degree of multiple detachment were observed. In some cases, the degree of improvement was also recognized in the group using the comparative example, but the degree was smaller than that in the group using the corresponding example.

As described above, the external preparations for skin of the examples of the present invention had an excellent effect of improving the aging symptoms and rough skin symptoms of the skin, as compared with the conventional comparative examples.

In addition, in the swallow moto plant extract 1 to the swallow moto plant extract 4 of the present invention, when stored at 10 ° C. or lower, epidermal cell activating action, dermal fibroblast activating action, collagen production for 6 months. The promoting effect was maintained with almost no change. In addition, in Examples 1 to 4 of the present invention, no change in state was observed when stored at 25 ° C for 6 months. Furthermore, in the above-mentioned actual use test, no panelists recognized skin irritation reaction or skin sensitization reaction in the use group of Example of the present invention, and irritation sensation such as pain and warmth, tingling sensation, and tingling sensation during use. Or, there was no panelist who complained of discomfort.

Example 5 Oil-in-Water Emulsion Ointment (1) White Vaseline 25.0 (2) Stearyl Alcohol 25.0 (3) Glycerin 12.0 (4) Sodium Lauryl Sulfate 1.0 (5) Tsubamemoto Genus plant extract 1 1.7 (6) Methyl paraoxybenzoate 0.1 (7) Purified water Amount with 100 as the total amount Production method: Mix the oil phase components of (1) to (4) and heat to homogeneity Melt to 75 ° C. On the other hand, the water phase components (5) to (7) are mixed and heated to homogenize the mixture to 75 ° C. The oil phase component is gradually added to this water phase component while stirring to emulsify and cool.

Example 6 Water-in-oil type emollient cream (1) Liquid paraffin 30.00 (2) Microcrystalline wax 2.00 (3) Vaseline 5.00 (4) Diglyceryl dioleate 5.00 (5) Sodium L-glutamate 1.60 (6) L-serine 0.40 (7) Propylene glycol 3.00 (8) Swallowtail plant extract 2 0.05 (9) Methyl paraoxybenzoate 0.10 ( 10) Purified water Amount with 100 as the total amount (11) Perfume 0.10 Manufacturing method: (5) and (6) were dissolved in a part of (10) to 50 ° C and preheated to 50 ° C (4 ) Is added slowly with stirring. This was mixed in advance and uniformly dispersed in (1) to (3) which was heated and dissolved at 70 ° C, and then (7) to (9).
What was melt | dissolved in the remainder of (10) and what was heated at 70 degreeC is added with stirring, and it emulsifies with a homomixer. After cooling
Add (11) at 50 ° C. and mix.

Example 7 Makeup Base Cream (1) Stearic Acid 12.00 (2) Cetanol 2.00 (3) Glyceryl Tri-2-ethylhexanoate 2.50 (4) Self-emulsifying Glyceryl Monostearin Acid ester 2.00 (5) Propylene glycol 10.00 (6) Potassium hydroxide 0.30 (7) Swallow moto plant extract 4 0.05 (8) Methyl paraoxybenzoate 0.10 (9) Purified water Total amount (10) Titanium oxide 1.00 (11) Red iron oxide 0.40 (12) Yellow iron oxide 0.10 (13) Perfume 0.10 Production method: The oil phase components of (1) to (4) are added. Mix and dissolve to 75 ° C. On the other hand, the water phase components (5) to (9) are mixed, heated and dissolved, and the pigment components (10) to (12) are added thereto, and the mixture is uniformly dispersed with a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, (13) is added and mixed at 40 ° C.

Example 8 Milky liquid foundation (1) Stearic acid 2.00 (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 (5) Decaglyceryl monoiso Palmitate 9.00 (6) 1,3-Butylene glycol 6.00 (7) Potassium hydroxide 0.08 (8) Methyl paraoxybenzoate 0.10 (9) Swallow moto plant extract 1 0.15 (10) Purified water Amount based on 100 (11) Titanium oxide 9.00 (12) Talc 7.40 (13) Red iron oxide 0.50 (14) Yellow iron oxide 1.10 (15) Black iron oxide 0. 10 (16) Perfume 0.15 Production method: Mix and dissolve the oil phase components (1) to (5) to 75 ° C. On the other hand, the aqueous phase components (6) to (10) are mixed, heated and dissolved, and the pigment components (11) to (15) are added thereto, and the mixture is uniformly dispersed with a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, (16) is added and mixed at 40 ° C.

Example 9 Set Lotion (1) Dimethyldiallylammonium Chloride / Acrylamide Copolymer 5.0 (2) Paraoxybenzoic Acid Ester 0.1 (3) Perfume 0.2 (4) Ethanol 30.0 ( 5) Purified water Total amount 100 (6) Swallow moto plant extract 3 3.0 (7) Glycerin 2.0 Manufacturing method: (1) to (4), (5) to (7) are mixed and dissolved, respectively. The ethanol and water phases. The aqueous phase is then added to the ethanol phase and mixed.

Example 10 Body Cleanser (1) Lauric Acid 9.00 (2) Myristic Acid 5.00 (3) Palmitic Acid 1.00 (4) Coconut Oil Fatty Acid Sarcosine Sodium 4.00 (5) Coconut oil fatty acid diethanolamide 1.00 (6) Purified water 100 (7) 1,3-butylene glycol 5.00 (8) Hydroxyethyl cellulose 0.50 (9) Potassium hydroxide 2.85 (10) ) Paraoxybenzoic acid ester 0.05 (11) Ethanol 2.00 (12) Perfume 0.10 (13) Swallow moto plant extract 4 1.00 Production method: (1) to (5) at 75 to 85 ° C. Then, the mixture is heated and dissolved, and to this, (6) to (11), which have been mixed and homogenized in advance and adjusted to 75 to 85 ° C., are added to completely saponify. Next, cooling is started, and at 45 to 50 ° C. (12), (13)
After adding, the mixture is further cooled to room temperature.

In addition, the extract of the genus Plant of the genus Swallow moto 1 to the extract of the plant of the genus Swallow moto 4 and Examples 1 to 10 are used as a composition for utilizing epidermal cells, a composition for utilizing dermal fibroblasts, and a composition for promoting collagen production. It can also be used to improve each symptom.

[0055]

INDUSTRIAL APPLICABILITY As described above in detail, according to the present invention, epidermal cells having excellent epidermal cell activating action, dermal fibroblast activating action, collagen production promoting action, and excellent stability and safety. It was possible to obtain an applied composition, a dermal fibroblast applied composition, a collagen production promoting composition, and a skin external preparation. Further, by further applying it, a skin external preparation capable of effectively preventing and improving the symptoms of skin aging and rough skin can be obtained.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 35/78 A61K 35/78 V A61P 17/00 A61P 17/00 (72) Inventor Hiroto Torii Hyogo 6-13-1 Minatojima Nakamachi, Chuo-ku, Kobe City Noevir Co., Ltd. Kobe Institute (72) Inventor Shinichi Fujimoto 2-3-1 Kudan Minami 2-chome, Chiyoda-ku, Tokyo F-term (High Performance Materials Department, GS AI Creos Co., Ltd.) (Reference) 4C083 AA082 AA111 AA112 AB032 AB052 AB232 AB242 AB432 AC012 AC022 AC072 AC122 AC242 AC342 AC392 AC422 AC442 AC482 AC582 AC642 AC662 AC782 AD132 AD282 AD512 AD662 CC02 CC04 CC05 CC12 CC23 DD23 DD27 DD31 DD32 DD33 DD45 EE11 4013 MA22 MA24 MA28 MA63 ZA89

Claims (6)

[Claims] 1. An epidermal cell activating composition comprising an extract of a plant of the genus Clintonia of the family Uvulariaceae . 2. A dermal fibroblast activating composition comprising an extract of a plant of the genus Clintonia of the family Uvulariaceae . 3. A composition for promoting collagen production, which comprises an extract of a plant of the genus Clintonia of the family Uvulariaceae . 4. The composition according to claim 1, wherein the plant extract of the genus Clintonia of the family Uvulariaceae is a water extract. 5. The plant of the genus Swallow's moto ( Clintonia ) of the family of Uvulariaceae is one or two selected from Clintonia borealis and Clintonia udensis. The composition according to claims 1 to 4. 6. An external preparation for skin, comprising the composition according to claim 1.