JP2003275734A - Apparatus for removing harmful chemical substance - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a harmful chemical substance-removing apparatus which efficiently removes a harmful chemical substance from an object contaminated with the harmful chemical substance. <P>SOLUTION: Soil 101 contaminated with dioxins is blended with iron powder in an agitating blender 102 and the contaminated soil with which the iron powder is blended is carried to an iron powder recovering apparatus 103. In the iron powder recovering apparatus 103, the iron powder is recovered by an energized electromagnet. Treated soil 106 from which the iron powder is removed is charged into an enzyme tank 107 storing an angular dioxygenase which is developed in a coil bacillus and then is extracted to perform enzyme treatment. By a series of these operations, the dioxins are efficiently removed and are detoxicated. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a harmful chemical substance removing apparatus for removing harmful chemical substances from a polluted object such as polluted soil.

[0002]

BACKGROUND ART In recent years, diffusion of harmful chemical substances such as heavy metals and organic chlorine compounds into the environment has become a social problem. The origin of these harmful chemical substances is industrial waste, pesticides, pesticides, electrical appliances, plastics, etc., but when they are caused by industrial waste, etc., they are particularly highly diffusible and have a strong adverse effect on the environment. Once soil and rivers are contaminated, they tend to be difficult to remove. Such harmful chemical substances include chemical substances called endocrine disruptors, which are so-called environmental hormones. As a method of removing harmful chemical substances from pollutants such as soil, combustion ash, wastewater, and leachate contaminated with such harmful chemical substances, physical treatment such as dissolution, chemical treatment such as electrolysis and redox Treatments, biological treatments such as microbial degradation, etc. have been used.

For example, dioxin is produced as a by-product in chemical substance manufacturing processes, waste incineration, paper mills, metal smelters, etc., and is also mixed as an impurity in pesticides and the like. In order to detoxify dioxin from the soil polluted with dioxin, ring opening by microorganisms and the like are used.

[0004]

However, the ring opening by microorganisms alone takes too much time, and the management burden such as temperature control is heavy. In addition, the easiness of ring cleavage varies greatly depending on the chemical structure, and the more chlorine groups, the more difficult it is for ring cleavage. Therefore, there is a problem that the efficiency of detoxifying dioxin is low.

Therefore, the present invention has been made for the purpose of providing a toxic chemical substance removing device for efficiently removing and detoxifying a toxic chemical substance from a polluted object such as soil polluted with the toxic chemical substance.

[0006]

A method for removing harmful chemical substances according to the present invention comprises a mixing means for mixing a metal adsorbing a harmful chemical substance with an object to be polluted contaminated with the harmful chemical substance, and the metal for the contamination. It is characterized by comprising: a recovery means for recovering after mixing with the target, and an enzyme treatment means for treating the contaminated object after the metal is recovered by the recovery means with an enzyme.

In addition, the enzyme is the following formula 1 or formula 2
The compound may be ring-opened.

[Formula 1]

[Formula 2]

However, R is H or halogen. Further, the halogen may be Cl. Further, the enzyme may be an angular dioxygenase. Further, the metal may be iron. Further, the metal may be recovered by a magnet. Further, the harmful chemical substance may be an organic chlorine compound. Further, the harmful chemical substance may be dioxin.

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below with reference to the drawings.

<Embodiment 1> FIG. 1 shows an example of the overall configuration of the hazardous chemical substance removing apparatus of the present invention. Here, an example of a dioxin removing device that removes dioxin from the contaminated soil 101 will be described by taking the contaminated soil 101 contaminated with dioxin as an example of a contamination target contaminated with harmful chemical substances. This device is a mixer / mixer 10 as a mixing means.
2 is equipped with an iron powder recovery device 103 as recovery means. The iron powder recovery device 103 includes an endlessly rotating belt, and a lower surface of a part of the iron powder recovery device 103 faces the belt conveyor 104 over a predetermined distance and a lower surface of another part of the iron powder recovery device 103 is made of iron. It extends above the powder recovery box 105. This iron powder recovery device 103 uses an electromagnet as a magnet for recovering iron powder on a belt, and the belt is energized in a portion other than the upper part of the iron powder recovery box 105, and the energization unit Then it fulfills the function of a magnet. further,
This apparatus is equipped with an enzyme tank 107 that further performs an enzyme treatment for ring-cleaving dioxin from the treated soil 106 after the contaminated soil 101 is treated with iron powder.

First, contaminated soil 1 contaminated with dioxins
01 is put into the agitator / mixer 102. Further, for example, 100 wt. Contaminated soil 10 in the agitator / mixer 102
1 and iron powder are mixed by vigorous shaking for 4 hours. During this time, dioxin is adsorbed on the iron powder.

Thereafter, the belt conveyor 104 conveys the contaminated soil 101 mixed with the iron powder to the iron powder recovery device 103. In the iron powder recovery device 103, only iron powder is adsorbed from the contaminated soil 101 on the belt conveyor 104 by the energized electromagnet. The iron powder adsorbed on the belt of the iron powder recovery device 103 is carried to the recovery box, but since no electric current flows in the belt above it, the magnetic force disappears, and the iron powder separates from the belt, and inside the recovery box 105. It is dropped and collected.

The treated soil 106 from which dioxin has been removed to some extent is conveyed as it is on the belt conveyor 104,
Next, it is put into the enzyme treatment tank 107 and subjected to the enzyme treatment. The enzyme treatment tank 107 is prepared as follows. First, the angular dioxygenase gene DbfA1A2 isolated from the Terrabacter bacterium DBF63 strain
Is cloned into an E. coli expression vector such as pET (trade name), and named pET-DfbA1A2. The pET vector can induce the expression of DfbA1A2 inserted downstream thereof by the IPTG-inducible T7lac promoter. For transformation of the pET vector, an Escherichia coli strain capable of supplying T7 RNA polymerase, for example, Tuner (DE3) pLacI strain (trade name) is used.

Tuner (DE3) p transformed with pET-DfbA1A2
The LacI strain is mass-cultured according to the protocol recommended by Novagen, and when DbfA1A2 is expressed by adding IPTG, angular dioxygenase is synthesized in Escherichia coli. Homogenizer (homogenizer) and sonicator (sonicator)
Escherichia coli is pulverized with, for example, and cell debris is removed by centrifugation or the like to obtain a culture solution containing a large amount of angular dioxygenase.

After the culture solution thus obtained is placed in the enzyme treatment tank 107, treated soil 106 from which dioxin has been removed to some extent using iron powder is introduced. The culture solution in the enzyme treatment tank 107 is kept for 3 hours for 18 hours.
Dioxins are detoxified by angular dioxygenase by maintaining at 0 ° C. and gently stirring.

In detoxifying dioxin, ring-opening tends to be less likely to occur as the number of chlorine atoms in the dioxin molecule is increased only by enzymatic treatment with an enzyme. On the contrary, when a dioxin molecule is adsorbed on iron, the more dioxin molecules have chlorine atoms, the easier it is to adsorb. Therefore, by sequentially performing these methods, all kinds of dioxins can be easily removed and rendered harmless.

<Embodiment 2> FIG. 2 shows another example of the entire structure of the hazardous chemical substance removing apparatus of the present invention. Here, as an object to be polluted with harmful chemical substances, contaminated water contaminated with dioxin will be taken as an example, and an example of a dioxin removing device for removing dioxin from this contaminated water will be described.
This device has a filter 202 having a pore size smaller than that of iron powder of 100 mesh on both ends of a column 201.
Is installed, and is filled only with iron powder.

Dioxin in the contaminated water can be removed to some extent by passing contaminated water contaminated with dioxin through this column and adsorbing the dioxin in the contaminated water to the iron powder.

After that, the treated water treated with iron powder is put into the enzyme treatment tank 107 containing the culture solution containing a large amount of angular dioxygenase in the same manner as in Example 1 to perform the enzyme treatment.

Also in this example, after the treatment with iron,
As in Example 1, it is possible to easily remove and detoxify all kinds of dioxins by sequentially performing the enzyme treatment.

<Other Embodiments> In the above embodiments, soil and water have been taken as examples of the objects to be contaminated, but the present invention is also applicable to ash such as incinerated ash and sand.

Although iron powder is taken as an example of the metal that adsorbs dioxin, a metal magnet, for example, an iron magnet may be used. In that case, not only the magnet but also the magnet is recovered.
A metal such as iron that attracts a magnet may be used.

The size of iron powder particles that adsorb dioxin was 100 mesh in the above example.
Considering the balance between adsorption efficiency and recovery efficiency, 60-30
It is preferably 0 mesh, but the shape and size are not particularly limited.

The addition rate of iron powder was set to 5%, but it is preferably 1 to 10% from the viewpoint of adsorption efficiency.

Although an electromagnet is used to recover the iron that has adsorbed dioxin, the invention is not limited to this, and a metal magnet or a plastic magnet may be used.

The enzyme for ring opening of dioxin is
Although it was produced using the angular dioxygenase gene DbfA1A2 isolated from the Terrabactor bacterium DBF63 strain, it may be produced using the dioxygenase gene isolated from the Pseudomonas strain DB10 strain,
Other enzymes that can decompose harmful chemical substances may be used.

The method for producing the dioxygenase by expressing the dioxygenase gene in E. coli is not limited to the pET vector system used here, but pGEX (trade name) and
An E. coli expression vector such as pFLAG (trade name) may be used, and a host bacterium may be a bacterium compatible with the vector.

Although a method of expressing the dioxygenase gene in Escherichia coli was used to produce dioxygenase, the bacterium expressing the enzyme such as dioxygenase is not limited to Escherichia coli, and the enzyme such as dioxygenase is also used. The enzyme may be produced by proliferating a bacterium having a gene encoding the enzyme and inducing the expression of the enzyme.

It is also possible to decompose the harmful chemical substance by mixing the microorganism expressing the enzyme with a harmful chemical substance such as dioxin without extracting the enzyme such as dioxygenase from the microorganism.

The toxic chemical substance is not limited to dioxins, and may be an organochlorine compound having a chlorine group such as trichlorethylene, or a toxic chemical substance other than the organochlorine compound.

Although other embodiments according to the present invention have been described above, the objects, features, advantages and ideas of the present invention will be apparent to those skilled in the art from the description of this specification.
Therefore, the above description of the embodiments of the invention and the description of specific examples and the like show preferred embodiments of the present invention, and are presented here only for the purpose of explanation. It should be understood that, within the spirit and scope of the invention disclosed herein, various alterations and modifications will be apparent to those skilled in the art based on their description.

[0031]

The present invention has been made for the purpose of providing a toxic chemical substance removing device for efficiently removing and detoxifying a toxic chemical substance from a polluted object such as soil polluted with the toxic chemical substance.

[Brief description of drawings]

FIG. 1 is an overall configuration diagram of a hazardous chemical substance removing device according to an embodiment of the present invention.

FIG. 2 is an overall configuration diagram of a hazardous chemical substance removing device according to another embodiment of the present invention.

[Explanation of symbols]

101 Contaminated soil 102 Stirrer / Mixer 103 Iron powder recovery device 104 belt conveyor 105 Collection Box 106 Treated soil treated with iron powder 107 enzyme treatment tank 201 column 202 filter

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) B09C 1/08 A62D 3/00 ZAB 4G066 C02F 1/28 C12N 9/02 1/58 B09B 3/00 304K C12N 15/09 5/00 S // A62D 3/00 ZAB C12N 15/00 A C12N 9/02 (71) Applicant 502101777 Hiroshi Habe 2-9-17 Kishimachi, Kita-ku, Tokyo Lions Mansion Higashijujo 3 Room 307 (72) Hirokazu Tsuji 4-640 Shimo-Seido, Kiyose-shi, Tokyo Inside the Obayashi Technical Research Institute Co., Ltd. (72) Inventor, consistently, 4-640 Shimo-Seido, Kiyose-shi Tokyo Metropolitan Institute F-Term (Obayashi Technical Research Institute) Reference) 4B024 AA03 AA17 BA08 CA02 DA06 4B050 CC03 DD02 FF01 LL05 4D004 AA16 AA36 AA41 AB07 CA09 CA15 CA20 CA47 CC07 CC11 4D024 AA10 AB11 BA14 B B01 BC01 CA01 DB18 4D038 AA10 AB14 BA04 BB06 4G066 AA02B BA20 CA33 DA08

Claims (8)

[Claims] 1. A mixing means for mixing a metal that adsorbs a harmful chemical substance with a contamination target contaminated with the harmful chemical substance, a recovery means for recovering the metal after mixing with the contamination target, and the recovery means. An apparatus for removing a harmful chemical substance, comprising: an enzyme treatment unit that treats the contamination target after the metal is recovered by an enzyme. 2. The hazardous chemical substance removing device according to claim 1, wherein the enzyme ring-opens a compound of the following formula 1 or formula 2. [Formula 1] [Formula 2] However, R is H or halogen. 3. The hazardous chemical substance removing device according to claim 2, wherein the halogen is Cl. 4. The hazardous chemical substance removing device according to claim 1, wherein the enzyme is an angular dioxygenase. 5. The harmful chemical substance removing device according to claim 1, wherein the metal is iron. 6. The hazardous chemical substance removing device according to claim 1, wherein the metal is recovered by a magnet. 7. The harmful chemical substance removing apparatus according to claim 1, wherein the harmful chemical substance is an organic chlorine compound. 8. The harmful chemical substance removing apparatus according to claim 1, wherein the harmful chemical substance is dioxin.