JP2002302414A - Sphingolipid structural matter-containing emulsified composition and method for producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a sphingolipid structural matter-containing emulsified composition usable without anxiety even about sensitive-skinned persons, with high penetrativeness into the skin and high moisturizing effect. SOLUTION: The sphingolipid structural matter-containing emulsified composition is obtained by the following procedure: an oil phase component containing a sphingolipid structural matter represented by ceramide is heated to about 80-90 deg.C and then forced into an SPG membrane 0.1-0.27 μm in pore size heated to about 80-90 deg.C to prepare emulsion microparticles uniform in size and highly excellent in stability with time. The composition comprising the thus obtained sphingolipid structural matter-containing emulsion microparticles is highly excellent in penetrativeness into the skin and moisturizing effect.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to cosmetics, pharmaceuticals,
In particular, the present invention relates to an emulsified composition containing a sphingolipid structural substance, which can be suitably used as a raw material for cosmetics or external preparations for medicated skin for enhancing the moisturizing property of the skin, and a method for producing the same.

[0002]

2. Description of the Related Art Sphingolipid structural substances represented by ceramide are main components of intercellular lipids in the stratum corneum, and are important for water retention of the skin and formation of a water-permeable barrier. It is known to play a role. For this reason, cosmetics and external preparations for medicated skin,
Furthermore, it has been formulated into a hair material or the like that maintains the gloss of hair (for example, JP-A-7-165690, JP-A-8-109).
121, JP-A-9-157149, JP-A-2000-
18613, JP-A-2000-256188, etc.).

[0003] Conventionally, solubilization and emulsification of ceramide and ceramide-like structural substances have been carried out by dissolving in oil phase components at a high temperature due to low solubility in oil phase components, and adding this during emulsification and stirring vigorously. Has been taken. However, when cooled after the completion of solubilization and emulsification, there are problems in terms of stability over time, such as precipitation as crystals when crystals are stored and binding of particles during storage. For this reason, for example, Japanese Patent Application Laid-Open
No. 88 proposes a method of adding an alkylloyl lactic acid ester salt to ceramides and dispersing them in water stably by the interaction of the two.

[0004] Furthermore, ceramide and ceramide-like structural substances do not penetrate efficiently into the stratum corneum of the skin, and have a problem that it is difficult to sufficiently exert a moisturizing effect. In order to solve this problem, Japanese Patent Application Laid-Open No. 7-33633 proposes a skin external preparation having a high healing effect by using ceramide in a liquid crystal state. Some have pointed out that it may irritate the skin and pose a safety problem. In addition, cosmetics often contain lower alcohols such as ethyl alcohol and isopropyl alcohol to enhance skin penetration, but those with weak skin such as alcohol sensitivity, atopic dermatitis, and senile xeroderma There was also a problem that could not be used with peace of mind.

The present invention has been made in view of the above-mentioned problems caused by sphingolipid structural substances represented by ceramide, and has excellent stability over time, and has excellent skin sensitivity such as alcohol sensitivity, atopic dermatitis and senile xerosis. An object of the present invention is to provide an emulsified composition containing a sphingolipid structural substance, which can be used safely by people with weakness and has high penetration into skin and excellent moisture retention.

[0006]

[Means for Solving the Problems] To solve such problems,
The present inventors have conducted intensive studies on a method of emulsifying an oil phase component containing a sphingolipid structural substance, and found that emulsification was performed using a microporous glass (SPG membrane) having a small pore size, which was not considered before. Thus, the present invention succeeded in producing an emulsified composition containing a sphingolipid structural material having excellent properties, and conceived the present invention based on such findings. That is, the present invention heats an oil phase component containing a sphingolipid structural substance to about 80 to 90 ° C,
Production of a sphingolipid-structured substance-containing emulsified composition including a step of preparing emulsion particles by injecting the heated oil phase component into an SPG membrane having a pore diameter of 0.1 μm to 0.2 μm heated to about 80 to 90 ° C. Is the way.

[0007] Microporous glass (SPG film) is made of Ca
This is a porous glass film using micro-phase separation of O-Al ₂ O ₃ -B ₂ O ₃ -SiO ₂ glass. When this SPG film is used to emulsify the film, the particle size can be adjusted according to the application. It is known that can be designed.

By using such a production method, for example, an SPG membrane having a pore size of about 0.1 μm to 0.2 μm is prepared, and while an aqueous phase component is heated and fluidized on one side, a sphingolipid structural material is contained. The oil phase component is heated to about 80-90 ° C., and pressure is applied to the heated oil phase component to about 80-90 ° C.
An O / W (oil-in-water) emulsion having a particle diameter of about 0.3 μm to 0.6 μm and a standard deviation of 0.1 μm or less can be obtained by press-fitting the SPG film heated at −90 ° C. However, the production method of the present invention requires
/ W emulsion, an oil component can be pressed into an SPG film emulsified, and the permeated oil phase component can be allowed to flow in an air stream to produce an O emulsion. W / O / W and O / W / O emulsions can also be prepared by injecting into emulsification.

According to the method for producing an emulsified composition containing a sphingolipid structural substance of the present invention, the particle diameter is extremely fine (about 0.3 μm to 0.6 μm) and uniform (for example, the standard deviation of the particle diameter is 0.1 μm or less). ) Can be obtained. Surprisingly, the sphingolipid-structured substance-containing emulsion particles are extremely excellent in stability over time without any change over time such as crystallization or particle size change due to bonding between particles. In addition, it has been found that the skin permeability and the moisture retention are clearly superior. Conventionally, in cosmetics and the like, a lower alcohol such as ethanol or isopropyl alcohol has been blended in order to enhance the penetration into the skin, but according to the present invention, even if such a lower alcohol is not contained, the skin can be added to the skin. Provides cosmetics and skin external preparations with excellent moisturizing properties that can enhance the penetration of the skin and can be used safely by people with weak skin such as alcohol sensitivity, atopic dermatitis, and senile xerosis. can do.

[0010] The emulsified composition obtained by the present invention includes, for example, cream, milky lotion, serum, lotion, pack, lotion for preventing rough skin, lotion for preventing sunshine, shaving cream, after shaving lotion, lipstick / lip cream, foundation , Hair cosmetics such as mascara, manicure, pressed powder, hair cream and skull prosthesis, raw materials for skin external preparation for treatment of skin diseases such as atopic dermatitis and senile xeroderma, etc.
It can be blended as an active ingredient in a composition that is expected to have a cosmetic action and a pharmaceutical action. Among them, the emulsified composition obtained by the present invention is excellent in permeability into the skin stratum corneum, and therefore, a skin application liquid, for example, an emulsion, an essence, a beauty liquid,
It is particularly excellent as an active ingredient in lotions, packs, lotions for preventing rough skin, lotions for preventing sunshine, shaving creams and after shaving lotions.

In the present invention, the term "sphingolipid structural substance" means a substance having a sphingolipid structure, and in addition to ceramides such as ceramides I to VI, derivatives of ceramides such as sphingosine and phytosphingosine and lipid precursor substances. And the like. The term "ceramides" means ceramide and these ceramide-like structures.

[0012]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described based on examples.

In this embodiment, a ceramide-containing O / W emulsion was produced by utilizing the SPG film emulsification method.

(Prescription) In this example, a ceramide-containing O / W emulsion was produced according to the following prescription. Oil phase components include N-stearoyl phytosphingosine, bird 2
-Glyceryl ethylhexanoate, POE (40) pyroglutamic acid isostearate, hydrogenated castor oil, sucrose stearic acid ester, natural vitamin E, 1,2-pentanediol, and 2-phenoxyethanol, 1,3 as an aqueous phase component Butylene glycol, acrylic acid / alkyl methacrylate copolymer, sodium hydroxide, and purified water were prepared.

Essence (100%) N-stearoyl phytosphingosine 0.2 glyceryl tri-2-ethylhexanoate 17.0 pyroglutamic acid isostearyl acid POE (40) hydrogenated castor oil 2.0 sucrose stearate 2.0 Natural vitamin E 0.05 1,2-pentanediol 2.0 2-phenoxyethanol 0.3 1,3 butylene glycol 2.0 Acrylic acid / alkyl methacrylate copolymer 0.2 Sodium hydroxide 0.05 Suitable amount of purified water (total 100%)

(Oil Phase Component) In this example, N-stearoylphytosphingosine, which is a ceramide derived from yeast, was used as a sphingolipid structural substance. However, the sphingolipid structural substance targeted by the present invention is not limited to such ceramides, but it may be pig skin, bovine brain,
Eggs, blood cells, natural ceramides of animal sphingolipids and plant sphingolipids obtained from natural extracts obtained from plants, synthetic ceramides, etc., regardless of their origin, ceramides I to VI, sphingosine, phytosphingosine, etc. Ceramide-like structures (ceramide derivatives, lipid precursors, etc. (JP-A-54-117421, JP-A-54-144308, JP-A-54-147937)
JP-A-62-228048, JP-A-63-216
852, etc.)).

Glyceryl tri-2-ethylhexanoate is
It is an oily component for dissolving ceramide. Examples of the oily component include hydrocarbon oils, ester oils, triglycerides, waxes, silicone oils, animal and vegetable oils, higher alcohols, fatty acids, fatty acid esters, polyhydric alcohol fatty acid esters, fatty acid octyldodecyl, polyglycerin fatty acid esters, Polyoxyethylene glycerin fatty acid ester, glycerin fatty acid ester, propylene glycol fatty acid ester, sugar fatty acid ester, lecithin derivative, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, and more specifically, for example, octyldodecanol, vaseline, ceresin, Beeswax, diisostearin, isostearic acid, diisopropyl adipate, isopropyl myristate, sunflower oil, rice oil, teaseed oil, evening primrose oil, kukui nut oil,
Used as a base material for cosmetics, pharmaceuticals, foods, etc., including vegetable oils such as rosehip oil, animal oils such as horse oil, mink oil and squalane, α-olefin oligomer, glyceryl tri (caprin / caprate), diisopropyl adipate Any one or more of the oily components used can be used in combination.

1,2-pentanediol is used as a crystallization inhibitor and a preservative. According to the present invention, it is possible to produce emulsion particles that are stable over time by emulsification using an SPG film having an extremely small diameter, so it can be said that there is no need to add a crystallization inhibitor, but it is safe as a product In this example, a crystallization inhibitor was intentionally added to provide it. As a crystallization inhibitor,
In addition to 1,2-pentanediol, for example, a polyhydric alcohol having 2 to 6 hydroxyl groups in a molecule, more specifically, glycerin, ethylene glycol, 1,3-butylene glycol, dipropylene glycol, propylene glycol, and isopropylene Glycol, sorbitol, 1,4
-Butylene glycol, hexylene glycol, 1,5
-One or a combination of two or more of pentanediol and the like can be used as appropriate. However, for those having no preservative effect, preservatives such as parabens and pionin are preferably added as appropriate.

Pyroglutamic acid isostearate POE
(40) Hardened castor oil and sucrose stearate are surfactants to help dissolve ceramide,
Other lipophilic surfactants can be used. Examples of lipophilic surfactants include, for example, natural surfactants such as sorbitan sesquiolate, glycerol monooleate, lanolin, cholesterol, soybean lecithin, egg yolk lecithin, polyethylene surfactants such as lipophilic polyglycerin fatty acid esters, nonionic Surfactants, synthetic surfactants and the like can be mentioned. Natural vitamin E is included as an antioxidant. In the present invention, since the oil phase component is heated to a high temperature in order to enhance the solubility of the ceramides, it is preferable to add an antioxidant. As the antioxidant, in addition to natural vitamin E, for example, one or a combination of two or more substances having an antioxidant effect such as tocophenol and BHT can be used. In the present invention, one or more oily active ingredients such as anti-inflammatory agents such as stearyl glycyrrhetinate, blood circulation promoters such as dl-α-tocopherol acetate, and physiologically active ingredients such as γ-oryzanol are also used. It can be used in combination.

(Aqueous phase component) 2-phenoxyethanol is used as a preservative. As the preservative, preservatives such as parabens, pionin, propionic acid, and glycine used in cosmetics, pharmaceuticals, foods, and the like can be appropriately added. 1,3-butylene glycol is used to enhance the preservative power, feeling of use, and moisture retention. Ceramide has the function of enhancing the moisturizing properties, but exhibits a more moisturizing effect when used in combination with other moisturizing agents. Examples of humectants include amino acids such as L-proline, polyhydric alcohols, organic acid salts such as diethylene glycol monoethyl ether, PCA (pyrrolidone carboxylate), lactic acid, hyaluronic acid and derivatives thereof, and mucopolysaccharides such as chondroitin sulfate. Water-soluble collagen, royal jelly extract, honey, propolis, placenta extract, chitin such as succinyl chitosan, chitosan and its derivatives, and the like. The acrylic acid / alkyl methacrylate copolymer is used as a thickener. Examples of the thickener include aliphatic monohydric alcohols having 1 to 3 carbon atoms, xanthan gum, carboxymethylcellulose salts, polyacrylic acid, sodium alginate, carboxylvinyl polymers, hyaluronic acid, carrageenan, etc. Thickeners such as conductive polymers can be used as well. Sodium hydroxide is used as a neutralizing agent and is used for cosmetics, pharmaceuticals,
PH adjusters used in foods and the like can also be used. The aqueous phase component in the present invention is optional, and various water-soluble components can be appropriately added in addition to water as needed. Examples of such a water-soluble component include a preservative, a compound having a function to enhance a feeling of use, a thickener, a neutralizer, an antibacterial agent, a humectant, and a stability improver. In addition, when making an anhydrous cream, it is not necessary to mix water.

(Emulsifying Apparatus) In the present embodiment, the SPG membrane emulsifying apparatus 1 (a small membrane emulsifying apparatus manufactured by Kiyomoto) shown in FIG.
Was used to emulsify. SPG (Shirasu Porous Glas
s) is a porous glass made of Shirasu volcanic ash, which has a unique porous structure in which pores are intertwined. The pore diameter is uniform and has the characteristic that the pore diameter can be controlled. FIG. 1 is a view showing the structure of an SPG membrane emulsifying apparatus 1, in which 2 is a disperse phase pressurized tank, 3 is an SPG module, 4 is an SPG membrane therein (in this embodiment, a pore diameter of 0.2 μm). SPG membrane was used.),
Reference numeral 5 denotes a continuous phase storage unit, and reference numeral 6 denotes a circulation pump. SP
The membrane emulsification method using G is described in SPG research papers (198
9) p35, introduced by SPG Study Group, Tadao Nakajima, Masataka Shimizu). As shown in FIG. 2, an aqueous phase is circulated inside the cylindrically formed SPG membrane 4 and the oil is circulated from the outside. When the oil phase permeates through the SPG membrane 4 and expands into a sphere of a certain size and separates from the SPG membrane 4, the O /
A W emulsion is formed.

(Manufacturing method) In the present embodiment, the dispersed phase pressurizing tank 2 and the SPG module 3 of the SPG film emulsifying apparatus 1 were previously heated to about 85 ° C. (Step 1). The above oil phase components (N-stearoyl phytosphingosine, bird 2
-Glyceryl ethylhexanoate, POE (40) pyroglutamate isostearate, hydrogenated castor oil, sucrose stearate, natural vitamin E, 1,2-pentanediol) were placed in a container and dissolved by heating at about 85 ° C (step 2). ). On the other hand, the aqueous phase component (2-phenoxyethanol, 1,3-butylene glycol, acrylic acid / alkyl methacrylate copolymer, sodium hydroxide, purified water)
Was dissolved in a separate container and dissolved by heating at about 75 ° C. (Step 3). Next, the aqueous phase component was put into the continuous phase storage section 5 of the SPG membrane emulsification apparatus 1 and circulated by driving the circulation pump 6, and the continuous phase was heated so as to maintain about 75 ° C. (Step 4). Then, the oil phase component was put into the dispersion phase pressurized tank 2, and nitrogen gas was fed at a pressure of 12 kgf / cm ² to perform membrane emulsification (step 5). The resulting emulsion composition (ceramide-containing O / W emulsion) was cooled to 38 ° C. in a water bath (step 6).

The obtained emulsified composition (ceramide-containing O / W
Emulsion) was clearly finer and more uniform than a normal ceramide-containing emulsion. Moreover, as a result of observation over time, no change over time such as crystallization or change in particle diameter due to bonding of particles was observed. Also,
As a result of a sensory test, it was found that the skin has excellent permeability and moisture retention.

In the above manufacturing method, the oil phase component, the dispersed phase pressurizing tank 2 and the SPG module 3 are all heated to about 85 ° C., but the same effect can be obtained even if the temperature is raised to about 80 to 90 ° C. It turned out to be obtained. On the other hand, if the temperature is lower than 80 ° C. by 5 ° C. or more, the ceramide may not be sufficiently dissolved and may not pass through the SPG membrane. May have undesirable effects. Further, in the above manufacturing method, an SPG film having a pore diameter of 0.2 μm was used.
Similar findings were obtained using a μm SPG membrane. In the manufacturing method of the present invention, the order of steps 1, 2, and 3 is arbitrary.

The emulsified composition obtained in this example, ie, a ceramide-containing emulsion, can be blended in cosmetics, pharmaceuticals, foods, and the like. For example, when used in cosmetics, additives commonly used in cosmetics such as ultraviolet absorbers, hair root activators, antioxidants, whitening agents, astringents, nutrients, fragrances, pigments, preservatives, chelating agents, etc. The agents can be combined and compounded. When used in quasi-drugs, in addition to the above additives, quasi-drug main ingredients such as anti-inflammatory agents, bactericides, keratolytic agents and the like can be combined and blended. When used for pharmaceuticals, it can be combined with various pharmaceutical ingredients such as anti-inflammatory agents, antihistamines, blood circulation promoters, hormonal agents, analgesics, and antipruritic agents in addition to the above-mentioned additives.

(Test 1) Emulsion particles of emulsion emulsified by using an SPG film (referred to as “SPG film method”) and emulsion particles of emulsion emulsified by using a vacuum emulsifier (referred to as “conventional method”) Were measured and compared.

(Preparation of Sample) In the conventional method, emulsification was carried out using a vacuum emulsifier (VQ-1-75 manufactured by Mizuho Industry Co., Ltd.). That is, an oil phase component (ceramides (N-stearoyl phytosphingosine) 0.2%, glyceryl tri-2-ethylhexanoate 17.0%, pyroglutamic acid isostearate POE (40) hydrogenated castor oil 2.0%,
2.0% sucrose fatty acid ester, natural vitamin E 0.0
5%, 1,2-pentanediol 2.0%) was dissolved in a dissolution tank by heating to 85 ° C, and this was dissolved in an aqueous phase component previously heated and dissolved at 75 ° C in an emulsifying apparatus ( 2-
Phenoxyethanol 0.3%, 1,3-butylene glycol 2.0%, acrylic acid / alkyl methacrylate copolymer 0.2%, sodium hydroxide 0.05%, purified water (appropriate amount)) Stir with a mixing mixer (number of rotations:
While cooling at 20 to 50 rpm, a comparative sample was obtained. In addition,% of each component composition is a mixing ratio when the total amount of the oil phase component and the water phase component is 100%.

In the SPG method, an SPG membrane emulsifier (a small membrane emulsifier manufactured by Kiyomoto, SPG membrane pore size: 0.2 μm)
Was used to emulsify. Dispersed phase pressurized tank and SPG
The module was previously heated to about 85 ° C., and the oil phase components (ceramides (N-stearoyl phytosphingosine) 0.2%, tri-2-ethylhexanoate glyceryl 17.0%, pyroglutamic acid isostearic acid) PO
E (40) hydrogenated castor oil 2.0%, sucrose fatty acid ester 2.0%, natural vitamin E 0.05%, 1,2-pentanediol 2.0%) in a container and heat-dissolved at about 85 ° C did. On the other hand, an aqueous phase component (0.3% of 2-phenoxyethanol, 2.0% of 1,3-butylene glycol, 0.2% of an acrylic acid / alkyl methacrylate copolymer, 0.05% of sodium hydroxide, purified water (appropriate amount) )) Was placed in a separate container, dissolved and heated and melted at about 75 ° C. In addition,% of each component composition is 1% of the total amount of the oil phase component and the aqueous phase component.
This is the mixing ratio when 00% is set. Next, this aqueous phase component is put into the continuous phase storage section of the SPG membrane emulsification apparatus, and is circulated by driving a circulation pump.
Was heated to keep Then, the oil phase component was put into a dispersion phase pressurized tank, and nitrogen gas was sent at a pressure of 12 kgf / cm ² to perform film emulsification. The resulting emulsified composition (ceramide-containing O / W emulsion) was cooled to 38 ° C. in a water bath to obtain an example sample.

0.5 ml of the comparative sample or 0.5 ml of the example sample was diluted with 9.5 ml of distilled water.
ml was sampled and measured with a laser analysis type particle size distribution analyzer (manufactured by Shimadzu Corporation: SALD-2000). The results are shown in FIGS.

(Test 2) Storage stability of emulsion emulsified using an SPG membrane (referred to as “SPG membrane method”) and emulsion emulsified using a vacuum emulsifier (referred to as “conventional method”) Were compared.

While the sample of the example and the sample of the comparative example obtained in the test 1 were stored at room temperature for one year without controlling the humidity, the sample of the example and the comparative example obtained in the test 1 were observed. The sample was placed in a thermo-hygrostat and stored for 6 months in an environment of 75% humidity and 40 ° C. for observation. At this time, observation of each sample was performed with the naked eye and a microscope (OLYMPUS
(Manufactured by Sharp Corporation: System Biological Microscope BX40).

As a result, when stored at room temperature, no change was observed with the naked eye in the sample of Example, and uniform particles were maintained when observed with a microscope. On the other hand, in the comparative sample, it was observed that the particles were separated into two layers with the naked eye, and the particles were observed to be in a non-uniform state with the microscope. When stored at a constant temperature and humidity, no change was observed with the naked eye in the sample of the example, and it was observed that uniform particles were maintained under a microscope. On the other hand, in the comparative example sample, two layers were separated with the naked eye, and a gel-like sediment was observed. From this, it was found that in the case of the example sample, even when stored in any environment, integration between particles and polymerization did not occur, a uniform particle diameter was maintained, and a stable emulsion over time was obtained.

(Test 3) The permeability between the emulsion emulsified using an SPG membrane (referred to as “SPG membrane method”) and the emulsion emulsified using a vacuum emulsifier (referred to as “conventional method”). Compared.

In the conventional method, emulsification was carried out using a vacuum emulsifying apparatus (VQ-1-75 manufactured by Mizuho Industry Co., Ltd.). That is, oil phase components (ceramides (ceramides I, II, III, VI or sphingosine) 0.02%, glyceryl tri-2-ethylhexanoate 0.8%, POE (40) pyroglutamic acid isostearate hydrogenated castor oil 1. 5%, sucrose fatty acid ester 0.1%, natural vitamin E 0.05%, 1,
2-pentanediol (2%) was heated to 85 ° C. in a dissolving tank to dissolve it,
Aqueous phase components (0.3% of 2-phenoxyethanol, 3.7% of 1,3 butylene glycol,
Acrylic acid / alkyl methacrylate copolymer 0.05
%, Sodium hydroxide 0.0125%, purified water (appropriate amount)) and stirred with a scraping mixer (rotation speed: 20
(50 rpm) to obtain a comparative sample. In addition,% of each component composition is a mixing ratio when the total amount of the oil phase component and the water phase component is 100%.

In the SPG method, an SPG membrane emulsifying apparatus (a small membrane emulsifying apparatus manufactured by Kiyomoto, SPG membrane pore size: 0.2 μm)
Was used to emulsify. Dispersed phase pressurized tank and SPG
The module was previously heated to about 85 ° C., and the oil phase components (ceramides (ceramide I, II, III, VI or sphingosine) 0.02%, tri-2-ethylhexanoate glyceryl 0.8%, pyroglutamic acid Isostearic acid POE
(40) Hardened castor oil 1.5%, sucrose fatty acid ester 0.1%, natural vitamin E 0.05%, 1,2-pentanediol 2%) were put in a container and dissolved by heating at about 85 ° C. On the other hand, the aqueous phase component (2-phenoxyethanol 0.3
%, 1,3 butylene glycol 3.7%, acrylic acid
0.05% of an alkyl methacrylate copolymer, 0.0125% of sodium hydroxide, and purified water (appropriate amount)) were placed in another container, dissolved and heated and dissolved at about 75 ° C. In addition,% of each component composition is 1% of the total amount of the oil phase component and the aqueous phase component.
This is the mixing ratio when 00% is set. Next, the aqueous phase component is put into the continuous phase storage section of the SPG membrane emulsifying apparatus, and a circulation pump is driven to circulate the solution.
Was heated to keep Then, the oil phase component was put into a dispersion phase pressurized tank, and nitrogen gas was fed at a pressure of 12 kgf / cm ² to perform membrane emulsification. The resulting emulsified composition (ceramide-containing O / W emulsion) was cooled to 38 ° C. in a water bath to obtain an example sample.

The permeability test was conducted on 26 healthy men and women (average 37.46 years) at room temperature of 22.1 ± 1.8 ° C.
The subject was kept at rest in an environment of a humidity of 30.2 ± 2.6% for 60 minutes, and the pre-epidermal water content of the epidermis before application of the emulsion was measured.
Next, 2 μL of each sample was taken, and 1 cm ×
The sample was applied to the 1 cm inner part of the forearm, and the water content of the stratum corneum was measured 5 minutes and 10 minutes after the application, and the results are summarized in FIG.

[0037]

[Table 1]

The measurement of the water content of the stratum corneum is carried out by a high-frequency impedance method ("Recent measurement method of skin moisture", Tatsuro Yamamura, "FRAG
RANCE JOURNAL ”1993-10 P36)
0 (manufactured by IBS). Incidentally, the high-frequency impedance method is currently the most widely used method for evaluating the moisture content on the skin surface.

As a result, it was found that, among ceramides, ceramides I, III and VI, especially ceramides I and III, among which ceramides I and III, had increased skin permeability, and ceramide III was most remarkable. .

[Brief description of the drawings]

FIG. 1 is a diagram showing a configuration of an SPG membrane emulsification device used in an embodiment of the present invention.

FIG. 2 is a diagram showing a state inside an SPG module in the apparatus of FIG. 1;

FIG. 3 is a graph showing an emulsion particle size distribution of an emulsion emulsified by an SPG film.

FIG. 4 is a graph showing an emulsion particle size distribution of an emulsion emulsified by a vacuum emulsification apparatus.

5 minutes or 1 minute after application of an emulsion emulsified by an SPG film (Example) or an emulsion emulsified by a vacuum emulsifier (conventional example)
It is the graph which showed the epidermis stratum corneum water content of 0 minutes.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 SPG membrane emulsification apparatus 2 Dispersed phase pressurized tank 3 SPG module 4 SPG membrane 5 Continuous phase storage part 6 Circulation pump

 ──────────────────────────────────────────────────続 き Continued on the front page F term (reference) 4C076 AA17 BB31 CC18 DD63N FF34 4C083 AB032 AC112 AC152 AC422 AC432 AC901 AC902 AD092 AD222 AD662 DD31 EE12 4G035 AB40 4G065 AA01 AB35Y AB40Y BB06 CA03 DA02 EA03 EA05 FA02

Claims (3)

[Claims] 1. An oil phase component containing a sphingolipid structural substance is heated to about 80 to 90 ° C.
Pore diameter 0.1μm-0.2μm heated to about 80-90 ° C
A method for producing an emulsified composition containing a sphingolipid structural material, comprising a step of preparing emulsion particles by press-fitting the composition into an SPG membrane. 2. An oil phase component containing a sphingolipid structural substance is heated to about 80 to 90 ° C., and the heated oil phase component is
Pore diameter 0.1μm-0.2μm heated to about 80-90 ° C
An emulsified composition containing a sphingolipid structural material containing emulsion particles obtained by press-fitting into an SPG membrane. 3. An emulsion containing a sphingolipid structural substance, wherein the average particle diameter of the sphingolipid structural substance-containing emulsion particles is 0.3 to 0.6 μm and the standard deviation of the particle diameter is 0.1 μm or less. Composition.