JP2001056438A5 - - Google Patents
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- JP2001056438A5 JP2001056438A5 JP2000169214A JP2000169214A JP2001056438A5 JP 2001056438 A5 JP2001056438 A5 JP 2001056438A5 JP 2000169214 A JP2000169214 A JP 2000169214A JP 2000169214 A JP2000169214 A JP 2000169214A JP 2001056438 A5 JP2001056438 A5 JP 2001056438A5
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Claims (19)
少なくとも試料と対物レンズのいずれか一方を光軸方向に移動させる移動手段と、
前記移動手段の移動により得られる複数のスライス像の同一座標の画素について光量が最大の画素の輝度情報と該輝度情報が得られる前記試料と対物レンズとの間の距離情報を検出する情報検出手段と、
前記情報検出手段により検出される画素ごとの前記距離情報に基づいて前記試料面の高さ情報を設定するとともに、前記情報検出手段により検出される画素ごとの前記輝度情報に基づいて該輝度情報に対しあらかじめ割り当てられている色情報を設定する三次元表示情報設定手段と、
この三次元表示情報設定手段で設定された高さ情報および色情報に基づいて三次元像を表示する表示手段とを具備したこと
を特徴とする共焦点走査型顕微鏡。In a confocal scanning microscope that scans spot light on the sample surface via an objective lens and receives reflected light from the sample surface,
Moving means for moving at least one of the sample and the objective lens in the optical axis direction;
Information detecting means for detecting the luminance information of the pixel having the maximum light quantity and the distance information between the sample and the objective lens from which the luminance information is obtained for the pixels at the same coordinates of the plurality of slice images obtained by the movement of the moving means When,
The height information of the sample surface is set based on the distance information for each pixel detected by the information detection unit, and the luminance information is set based on the luminance information for each pixel detected by the information detection unit. 3D display information setting means for setting color information assigned in advance,
A confocal scanning microscope comprising: display means for displaying a three-dimensional image based on height information and color information set by the three-dimensional display information setting means.
を特徴とする請求項１記載の共焦点走査型顕微鏡。The three-dimensional display information setting unit uses color information obtained by mixing, as color information, luminance information of a pixel whose light amount detected by the information detection unit is maximum and shadow information calculated from a visual field position and a light source position. The confocal scanning microscope according to claim 1.
を特徴とする請求項１記載の共焦点走査型顕微鏡。The three-dimensional display information setting means includes color information preliminarily allocated to the luminance information based on the luminance information and color information preliminarily allocated to the distance information based on the distance information. The confocal scanning microscope according to claim 1, wherein color information obtained by mixing the color information is used.
を特徴とする請求項１記載の共焦点走査型顕微鏡。The three-dimensional display information setting means includes color information preliminarily allocated to the luminance information based on the luminance information and color information preliminarily allocated to the distance information based on the distance information. The confocal scanning microscope according to claim 1, wherein color information obtained by adjusting a mixing ratio according to the luminance information is used.
を特徴とする請求項１記載の共焦点走査型顕微鏡。The three-dimensional display information setting means includes color information preliminarily allocated to the luminance information based on the luminance information and color information preliminarily allocated to the distance information based on the distance information. The confocal scanning microscope according to claim 1, wherein color information obtained by adjusting a mixing ratio according to the distance information is used.
を特徴とする請求項１記載の共焦点走査型顕微鏡。Furthermore, it has a contrast changing means for changing the contrast of the luminance information, and the three-dimensional display information setting means changes the contrast of the luminance information by the contrast changing means and is previously assigned to the luminance information. 2. The confocal scanning microscope according to claim 1, wherein color information is used.
前記試料と対物レンズとの相対的移動により得られる複数のスライス像の同一座標の画素について光量が最大の画素の輝度情報と該輝度情報が得られる前記試料と対物レンズとの間の距離情報を検出する第１の工程と、
前記第１の工程により検出される画素ごとの前記距離情報に基づいた前記試料面の高さ情報と、前記情報検出手段により検出される画素ごとの前記輝度情報に基づいて該輝度情報に対しあらかじめ割り当てられている色情報を三次元表示情報として設定する第２の工程と、を具備し、
前記第２の工程の三次元表示情報による高さ情報および色情報に基づいて三次元像を表示させること
を特徴とする共焦点走査型顕微鏡における画像表示方法。A confocal scanning type in which at least one of the sample and the objective lens is moved in the optical axis direction, spot light is scanned on the sample surface via the objective lens, and reflected light from the sample surface is received. An image display method in a microscope,
Luminance information of the pixel with the maximum light quantity and distance information between the sample and the objective lens from which the luminance information is obtained for pixels at the same coordinates of a plurality of slice images obtained by relative movement between the sample and the objective lens A first step of detecting;
The height information of the sample surface based on the distance information for each pixel detected by the first step and the luminance information based on the luminance information for each pixel detected by the information detection unit A second step of setting the assigned color information as three-dimensional display information,
An image display method in a confocal scanning microscope, wherein a three-dimensional image is displayed based on height information and color information based on three-dimensional display information in the second step.
を特徴とする請求項７記載の共焦点走査型顕微鏡における画像表示方法。In the second step, as the color information of the three-dimensional display information, the luminance information of the pixel having the maximum light amount detected in the first step is mixed with the shadow information calculated from the visual field position and the light source position. 8. The image display method in a confocal scanning microscope according to claim 7, wherein color information is used.
を特徴とする請求項７記載の共焦点走査型顕微鏡における画像表示方法。In the second step, as color information in the three-dimensional display information, color information previously assigned to the luminance information based on the luminance information is preliminarily assigned to the distance information based on the distance information. 8. The image display method in a confocal scanning microscope according to claim 7, wherein color information obtained by mixing the assigned color information is used.
を特徴とする請求項７記載の共焦点走査型顕微鏡における画像表示方法。In the second step, as color information in the three-dimensional display information, color information previously assigned to the luminance information based on the luminance information is preliminarily assigned to the distance information based on the distance information. 8. The method of displaying an image in a confocal scanning microscope according to claim 7, wherein the allocated color information is color information obtained by mixing the color information by adjusting a mixing ratio according to the luminance information.
を特徴とする請求項７記載の共焦点走査型顕微鏡における画像表示方法。In the second step, as color information in the three-dimensional display information, color information previously assigned to the luminance information based on the luminance information is preliminarily assigned to the distance information based on the distance information. 8. The method of displaying an image in a confocal scanning microscope according to claim 7, wherein the assigned color information is color information obtained by mixing the color information by adjusting a mixing ratio according to the distance information.
を特徴とする請求項７記載の共焦点走査型顕微鏡における画像表示方法。8. The second step uses color information preliminarily assigned to the luminance information while changing contrast of the luminance information as color information of the three-dimensional display information. Image display method in a confocal scanning microscope.
前記試料と対物レンズとの相対的移動により得られる複数のスライス像の同一座標の画素について光量が最大の画素の輝度情報と該輝度情報が得られる前記試料と対物レンズとの間の距離情報を検出し、この検出される画素ごとの前記距離情報に基づいて前記試料面の高さ情報を設定するとともに、前記検出される画素ごとの前記輝度情報に基づいて該輝度情報に対しあらかじめ割り当てられている色情報を三次元表示情報として設定し、この設定された高さ情報および色情報に基づいて三次元像を表示させることを特徴とする画像表示処理をするための処理プログラムを記録した記録媒体。A confocal scanning type in which at least one of the sample and the objective lens is moved in the optical axis direction, spot light is scanned on the sample surface via the objective lens, and reflected light from the sample surface is received. A recording medium recording a processing program for image display processing by a computer used in a microscope,
Luminance information of the pixel with the maximum light quantity and distance information between the sample and the objective lens from which the luminance information is obtained for pixels at the same coordinates of a plurality of slice images obtained by relative movement of the sample and the objective lens Detecting and setting height information of the sample surface based on the distance information for each detected pixel, and pre-assigned to the luminance information based on the luminance information for each detected pixel Recording medium storing a processing program for image display processing, characterized in that the color information is set as three-dimensional display information and a three-dimensional image is displayed based on the set height information and color information .
を特徴とする請求項１３記載の画像表示処理をするための処理プログラムを記録した記録媒体。The color information of the three-dimensional display information is color information obtained by mixing shade information calculated from a visual field position and a light source position with luminance information of the pixel having the maximum detected light amount. A recording medium on which a processing program for performing the image display processing according to 13 is recorded.
を特徴とする請求項１３記載の画像表示処理をするための処理プログラムを記録した記録媒体。As color information in the three-dimensional display information, color information pre-assigned to the luminance information based on the luminance information, color information pre-assigned to the distance information based on the distance information, 14. The recording medium recording a processing program for performing image display processing according to claim 13, wherein mixed color information is used.
を特徴とする請求項１３記載の画像表示処理をするための処理プログラムを記録した記録媒体。As color information in the three-dimensional display information, color information pre-assigned to the luminance information based on the luminance information, color information pre-assigned to the distance information based on the distance information, 14. A recording medium recording a processing program for performing image display processing according to claim 13, wherein color information mixed by adjusting a mixing ratio according to the luminance information is used.
を特徴とする請求項１３記載の画像表示処理をするための処理プログラムを記録した記録媒体。As color information in the three-dimensional display information, color information pre-assigned to the luminance information based on the luminance information, color information pre-assigned to the distance information based on the distance information, 14. The recording medium recording a processing program for performing image display processing according to claim 13, wherein color information mixed by adjusting a mixing ratio according to the distance information is used.
を特徴とする請求項１３記載の画像表示処理をするための処理プログラムを記録した記録媒体。14. The image display process according to claim 13, wherein contrast is changed for the luminance information as the color information of the three-dimensional display information, and color information previously assigned to the luminance information is used. A recording medium on which a processing program is recorded.
少なくとも試料と対物レンズのいずれか一方を光軸方向に移動させる移動手段と、Moving means for moving at least one of the sample and the objective lens in the optical axis direction;
前記移動手段の移動により得られる複数のスライス像の同一座標の画素について光量が最大の画素の輝度情報と該輝度情報が得られる前記試料と対物レンズとの間の距離情報を検出する情報検出手段と、Information detecting means for detecting the luminance information of the pixel having the maximum light quantity and the distance information between the sample and the objective lens from which the luminance information is obtained for the pixels at the same coordinates of the plurality of slice images obtained by the movement of the moving means. When,
前記情報検出手段により検出される画素ごとの前記距離情報に基づいて前記試料面の高さ情報を設定するとともに、該カラー共焦点走査型顕微鏡から得られる色情報をそのまま色情報として設定する三次元表示情報設定手段と、A three-dimensional setting that sets the height information of the sample surface based on the distance information for each pixel detected by the information detection means, and sets the color information obtained from the color confocal scanning microscope as it is as color information Display information setting means;
この三次元表示情報設定手段で設定された高さ情報および色情報に基づいて三次元像を表示する表示手段とを具備したことDisplay means for displaying a three-dimensional image based on height information and color information set by the three-dimensional display information setting means
を特徴とするカラー共焦点走査型顕微鏡。A color confocal scanning microscope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000169214A JP4229573B2 (en) | 1999-06-09 | 2000-06-06 | Confocal scanning microscope, image display method in confocal scanning microscope, and recording medium recording processing program for image display processing |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16243499 | 1999-06-09 | ||
| JP11-162434 | 1999-06-09 | ||
| JP2000169214A JP4229573B2 (en) | 1999-06-09 | 2000-06-06 | Confocal scanning microscope, image display method in confocal scanning microscope, and recording medium recording processing program for image display processing |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2001056438A JP2001056438A (en) | 2001-02-27 |
| JP2001056438A5 true JP2001056438A5 (en) | 2005-10-27 |
| JP4229573B2 JP4229573B2 (en) | 2009-02-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2000169214A Expired - Fee Related JP4229573B2 (en) | 1999-06-09 | 2000-06-06 | Confocal scanning microscope, image display method in confocal scanning microscope, and recording medium recording processing program for image display processing |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4511105B2 (en) * | 2001-07-04 | 2010-07-28 | オリンパス株式会社 | Scanning laser microscope and external signal recording method |
| WO2003038503A1 (en) * | 2001-11-02 | 2003-05-08 | Olympus Corporation | Scanning con-focal microscope |
| JP4994587B2 (en) * | 2004-10-27 | 2012-08-08 | オリンパス株式会社 | Image display method, program, and scanning confocal microscope |
| JP2007286147A (en) * | 2006-04-13 | 2007-11-01 | Jasco Corp | Infrared microscope |
| JP5716459B2 (en) * | 2011-03-02 | 2015-05-13 | 国立大学法人 新潟大学 | Cam surface observation method |
| JP5918658B2 (en) | 2012-08-29 | 2016-05-18 | 株式会社日立エルジーデータストレージ | Optical device |
| CN107065160A (en) * | 2017-06-07 | 2017-08-18 | 李昕昱 | A kind of full-automatic micro- scanner |
| CN111238383B (en) * | 2020-01-21 | 2021-10-12 | 武汉工程大学 | Colloid three-dimensional reconstruction and thickness measurement method and system based on spectrum confocal |
| WO2023053238A1 (en) * | 2021-09-29 | 2023-04-06 | 日本電気株式会社 | Shape detecting device, shape detecting system, and shape detecting method |
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- 2000-06-06 JP JP2000169214A patent/JP4229573B2/en not_active Expired - Fee Related
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