JP2000515377A - ｐＨ最適値、基質特異性及び熱安定性を増大するためのグルコアミラーゼのタンパク質操作 - Google Patents

ｐＨ最適値、基質特異性及び熱安定性を増大するためのグルコアミラーゼのタンパク質操作

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Publication number
JP2000515377A
JP2000515377A JP10507223A JP50722398A JP2000515377A JP 2000515377 A JP2000515377 A JP 2000515377A JP 10507223 A JP10507223 A JP 10507223A JP 50722398 A JP50722398 A JP 50722398A JP 2000515377 A JP2000515377 A JP 2000515377A
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JP
Japan
Prior art keywords
mutation
glucoamylase
mutations
mutant
reduced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP10507223A
Other languages
English (en)
Japanese (ja)
Inventor
マーティン アレン
ツェイ ユン ファン
ユーシン リ
スアン リーアン リウ
シウ メイ チェン
ペドロ クータンオー
リチャード ホンザトコ
クラーク フォード
Original Assignee
アイオワ ステイト ユニヴァーシティ リサーチ ファウンデーション インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アイオワ ステイト ユニヴァーシティ リサーチ ファウンデーション インコーポレイテッド filed Critical アイオワ ステイト ユニヴァーシティ リサーチ ファウンデーション インコーポレイテッド
Publication of JP2000515377A publication Critical patent/JP2000515377A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP10507223A 1996-07-24 1997-07-24 ｐＨ最適値、基質特異性及び熱安定性を増大するためのグルコアミラーゼのタンパク質操作 Pending JP2000515377A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US2257896P 1996-07-24 1996-07-24
US60/022,578 1996-07-24
US2307796P 1996-08-02 1996-08-02
US60/023,077 1996-08-02
PCT/US1997/012983 WO1998003639A1 (en) 1996-07-24 1997-07-24 PROTEIN ENGINEERING OF GLUCOAMYLASE TO INCREASE pH OPTIMUM, SUBSTRATE SPECIFICITY AND THERMOSTABILITY

Publications (1)

Publication Number Publication Date
JP2000515377A true JP2000515377A (ja) 2000-11-21

Family

ID=26696092

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10507223A Pending JP2000515377A (ja) 1996-07-24 1997-07-24 ｐＨ最適値、基質特異性及び熱安定性を増大するためのグルコアミラーゼのタンパク質操作

Country Status (6)

Country Link
EP (1) EP0970193A1 (zh)
JP (1) JP2000515377A (zh)
CN (1) CN1238009A (zh)
AU (1) AU3892397A (zh)
CA (1) CA2259958A1 (zh)
WO (1) WO1998003639A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013502211A (ja) * 2009-08-19 2013-01-24 ダニスコ・アクティーゼルスカブ グルコアミラーゼの変異体

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537792B1 (en) * 1996-07-24 2003-03-25 Iowa State University Protein engineering of glucoamylase to increase pH optimum, substrate specificity and thermostability
KR20010032497A (ko) * 1997-11-26 2001-04-25 에이.이. 스테일리 매니팩츄어링 컴파니 막 분리 단계를 포함하는 효소성 전분 당화
US6352851B1 (en) 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
EP1914306A3 (en) * 1998-07-15 2008-09-10 Novozymes A/S Glucoamylase Variants
EP1097196B2 (en) * 1998-07-15 2011-04-20 Novozymes A/S Glucoamylase variants
WO2000075296A1 (en) * 1999-06-02 2000-12-14 Novozymes A/S Novel glucoamylase
CA2374009A1 (en) 1999-07-09 2001-01-18 Novozymes A/S Glucoamylase variant
WO2003029449A2 (en) 2001-10-01 2003-04-10 Novozymes A/S Glucoamylase variants
CN102994474B (zh) * 2012-12-31 2015-04-15 江南大学 一种热稳定性提高的淀粉酶突变体及其应用
CN103409392B (zh) * 2013-07-25 2015-06-03 江南大学 一种热稳定的淀粉酶突变体及其制备方法和应用
CN105960176A (zh) 2014-02-07 2016-09-21 诺维信公司 用于生产葡萄糖糖浆的组合物
WO2020239064A1 (zh) * 2019-05-31 2020-12-03 南京百斯杰生物工程有限公司 一种热稳定性葡萄糖氧化酶
CN114381448B (zh) * 2022-01-10 2024-02-20 鑫缘茧丝绸集团股份有限公司 一种葡聚糖酶突变体及其应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013502211A (ja) * 2009-08-19 2013-01-24 ダニスコ・アクティーゼルスカブ グルコアミラーゼの変異体

Also Published As

Publication number Publication date
CA2259958A1 (en) 1998-01-29
CN1238009A (zh) 1999-12-08
AU3892397A (en) 1998-02-10
WO1998003639A1 (en) 1998-01-29
EP0970193A1 (en) 2000-01-12

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