IL135242A0 - Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna - Google Patents

Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna

Info

Publication number
IL135242A0
IL135242A0 IL13524298A IL13524298A IL135242A0 IL 135242 A0 IL135242 A0 IL 135242A0 IL 13524298 A IL13524298 A IL 13524298A IL 13524298 A IL13524298 A IL 13524298A IL 135242 A0 IL135242 A0 IL 135242A0
Authority
IL
Israel
Prior art keywords
expression
gene
genes
methods
nucleic acid
Prior art date
Application number
IL13524298A
Other languages
English (en)
Original Assignee
Athersys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Athersys Inc filed Critical Athersys Inc
Publication of IL135242A0 publication Critical patent/IL135242A0/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
IL13524298A 1997-09-26 1998-09-25 Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna IL135242A0 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94122397A 1997-09-26 1997-09-26
US15964398A 1998-09-24 1998-09-24
PCT/US1998/020094 WO1999015650A1 (en) 1997-09-26 1998-09-25 Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna

Publications (1)

Publication Number Publication Date
IL135242A0 true IL135242A0 (en) 2001-05-20

Family

ID=26856150

Family Applications (2)

Application Number Title Priority Date Filing Date
IL13524298A IL135242A0 (en) 1997-09-26 1998-09-25 Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna
IL135242A IL135242A (en) 1997-09-26 2000-03-23 Methods for creating a cell that produces a protein product of an endogenous gene, cloned and isolated eukaryotic cells that express a protein encoded by an endogenous gene and their use

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL135242A IL135242A (en) 1997-09-26 2000-03-23 Methods for creating a cell that produces a protein product of an endogenous gene, cloned and isolated eukaryotic cells that express a protein encoded by an endogenous gene and their use

Country Status (15)

Country Link
EP (1) EP1017803B1 (ja)
JP (1) JP5377806B2 (ja)
KR (1) KR20010024296A (ja)
CN (1) CN100582230C (ja)
AT (1) ATE471373T1 (ja)
AU (1) AU750025B2 (ja)
BR (1) BR9812540A (ja)
CA (1) CA2304642C (ja)
DE (1) DE69841723D1 (ja)
DK (1) DK1017803T3 (ja)
HK (1) HK1027375A1 (ja)
IL (2) IL135242A0 (ja)
NO (1) NO20001497L (ja)
NZ (1) NZ504185A (ja)
WO (1) WO1999015650A1 (ja)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7316923B1 (en) 1997-09-26 2008-01-08 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
CN1384881A (zh) * 1999-02-19 2002-12-11 阿瑟西斯公司 非靶向活化内源基因的组合物和方法
EP1305412A2 (en) 1999-10-14 2003-05-02 Clontech Laboratories Inc. Anthozoa derived chromo/fluoroproteins and methods for using the same
CA2413673C (en) 2000-06-28 2018-01-09 Amgen Inc. Thymic stromal lymphopoietin receptor molecules and uses thereof
GB0018876D0 (en) * 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
WO2002036760A2 (en) * 2000-10-30 2002-05-10 Novozymes Biotech, Inc. Methods for expressing endogenous genes by restriction enzyme mediated integration
EP1451295B1 (en) * 2001-11-02 2015-06-03 Athersys, Inc. Methods for mutating genes in cells and animals using insertional mutagenesis
DE60233347D1 (de) 2001-12-19 2009-09-24 Univ Chicago Schnellreifende fluoreszierende proteine und verfahren zu deren anwendung
US7662924B2 (en) 2002-02-22 2010-02-16 The Board Of Trustees Of The University Of Illinois Beta chain-associated regulator of apoptosis
NZ538464A (en) 2002-08-21 2008-01-31 Revivicor Inc Porcine animals lacking any expression of functional alpha 1,3 galactosyltransferase
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US8021833B2 (en) 2003-02-12 2011-09-20 Functional Genetics, Inc. Method for reducing HIV viral budding by administering a VPS28-specfic antibody that disrupts Gag-TSG101-VPS28 binding interactions
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
KR20070002057A (ko) 2004-03-17 2007-01-04 레비비코르 인코포레이션 기능성 알파 1,3 갈락토실트랜스펙션스페라제의 어떠한발현도 결여된 동물로부터 유래된 조직 산물
JP4755174B2 (ja) 2004-04-07 2011-08-24 ザ ユニヴァーシティー オヴ シカゴ 単量体赤色蛍光タンパク質
EP1774017B1 (en) 2004-07-26 2013-05-15 Pfenex Inc. Process for improved protein expression by strain engineering
CN101389214A (zh) 2004-10-22 2009-03-18 雷维维科公司 具有基因修饰免疫系统的有蹄类动物
PA8660701A1 (es) 2005-02-04 2006-09-22 Pfizer Prod Inc Agonistas de pyy y sus usos
ES2772674T3 (es) 2005-05-12 2020-07-08 Zymogenetics Inc Composiciones y métodos para modular respuestas inmunitarias
WO2007035213A2 (en) 2005-08-09 2007-03-29 Revivicor, Inc. Transgenic ungulates expressing ctla4-ig and uses thereof
US7749704B2 (en) 2005-11-01 2010-07-06 Mayo Foundation For Medical Education And Research Promoter polymorphisms of the BLyS gene and use in diagnostic methods
US8014957B2 (en) 2005-12-15 2011-09-06 Fred Hutchinson Cancer Research Center Genes associated with progression and response in chronic myeloid leukemia and uses thereof
KR101193722B1 (ko) 2006-07-24 2013-01-11 바이오렉시스 파마슈티칼 코포레이션 엑센딘 융합 단백질
CA2685326A1 (en) 2007-04-27 2008-11-06 Dow Global Technologies Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US8679749B2 (en) 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
WO2009073511A2 (en) 2007-11-30 2009-06-11 Mayo Foundation For Medical Education And Research Polymorphisms of the blys gene and use in diagnostic methods
EP2525808A2 (en) 2010-01-19 2012-11-28 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for male reproductive disorders
CN102669057B (zh) * 2011-03-18 2014-11-26 上海南方模式生物科技发展有限公司 一种调控动物内源基因表达的遗传修饰方法
KR101948248B1 (ko) * 2011-11-18 2019-02-14 한국생명공학연구원 단백질 발현 미세 조절방법
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
US10052364B2 (en) 2013-03-15 2018-08-21 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for cognitive disorders
HUE065691T2 (hu) * 2014-03-14 2024-06-28 Cibus Us Llc Eljárások és készítmények célzott génmódosítás hatékonyságának növelésére oligonukleotid-közvetített génjavítás alkalmazásával
US11191811B2 (en) 2014-11-19 2021-12-07 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for frailty associated with aging
CA3100376A1 (en) * 2018-05-15 2019-11-21 Duke University Systems and methods for genetic manipulation of akkermansia species

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
ATE174058T1 (de) * 1989-11-06 1998-12-15 Cell Genesys Inc Herstellung von proteinen mittels homologer rekombination
US5204939A (en) * 1989-12-14 1993-04-20 Fujitsu Limited Rule base processing system and rule evaluation control method therein
US5272071A (en) * 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6270989B1 (en) * 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
US5561053A (en) * 1994-08-05 1996-10-01 Genentech, Inc. Method for selecting high-expressing host cells
CN1384881A (zh) * 1999-02-19 2002-12-11 阿瑟西斯公司 非靶向活化内源基因的组合物和方法

Also Published As

Publication number Publication date
JP2003535564A (ja) 2003-12-02
JP5377806B2 (ja) 2013-12-25
WO1999015650A9 (en) 2000-09-08
EP1017803A1 (en) 2000-07-12
IL135242A (en) 2011-06-30
CN100582230C (zh) 2010-01-20
CA2304642A1 (en) 1999-04-01
AU750025B2 (en) 2002-07-11
DE69841723D1 (de) 2010-07-29
AU9581698A (en) 1999-04-12
CA2304642C (en) 2014-11-18
HK1027375A1 (en) 2001-01-12
BR9812540A (pt) 2001-12-26
CN1280615A (zh) 2001-01-17
NZ504185A (en) 2003-02-28
ATE471373T1 (de) 2010-07-15
NO20001497L (no) 2000-05-25
EP1017803B1 (en) 2010-06-16
NO20001497D0 (no) 2000-03-22
WO1999015650A1 (en) 1999-04-01
EP1017803A4 (en) 2002-01-09
DK1017803T3 (da) 2010-08-23
KR20010024296A (ko) 2001-03-26

Similar Documents

Publication Publication Date Title
IL135242A0 (en) Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna
SG141233A1 (en) Control of gene expression
WO2000049162A8 (en) Compositions and methods for non-targeted activation of endogenous genes
MX9606476A (es) Inhibicion citoplasmica de expresion de genes.
AU7599500A (en) Novel gl50 molecules and uses therefor
ATE171212T1 (de) Corticotropin freisetzende rezeptoren des faktor 2
AU5559499A (en) Identification of the gene causing the mouse scurfy phenotype and its human ortholog
AU6724794A (en) GRB3-3 gene, its variants and their uses
WO2002002760A3 (en) 25869, a human carboxylesterase and uses thereof
AU2002320301A1 (en) Novel pgc-1 isoforms and uses therefor
WO2001092495A3 (en) A human lysyl ixodase (47765) and uses thereof
AU2294102A (en) Mutant muscle-specific enhancers
WO2001079489A8 (en) 39228, a human alcohol dehydrogenase and uses therefor
WO2001075121A3 (en) 16836, a human phospholipase c family member and uses thereof
CA2487290A1 (en) Ehd1 gene promoting plant flowering and utilization thereof
WO2001075076A3 (en) 33167, a novel human hydrolase and uses therefor
WO2002006485A3 (en) 47885, a novel human ubiquitin-activating enzyme and uses therefor
AU2001253258A1 (en) Human trp-like calcium channel protein-2 (tlcc-2)
WO2001081584A3 (en) 27960, a novel ubiquitin conjugating enzyme family member and uses therefor
WO2001090145A3 (en) 57805, a human cadherin family member and uses thereof
AU2002234132A1 (en) 13245, a novel human myotonic dystrophy type protein kinase and uses therefor
AU2001285204A1 (en) Atcr-1, a human acyltransferase and uses thereof
WO2002029037A3 (en) 57800, a novel human cadherin and uses therefor
WO2001074854A3 (en) 32449, a putative human transporter and uses thereof
WO2001079293A3 (en) Human cadherin molecules and uses therefor