IL114118A0 - Novel cell systems having specific interaction of peptide binding pairs - Google Patents
Novel cell systems having specific interaction of peptide binding pairsInfo
- Publication number
- IL114118A0 IL114118A0 IL11411895A IL11411895A IL114118A0 IL 114118 A0 IL114118 A0 IL 114118A0 IL 11411895 A IL11411895 A IL 11411895A IL 11411895 A IL11411895 A IL 11411895A IL 114118 A0 IL114118 A0 IL 114118A0
- Authority
- IL
- Israel
- Prior art keywords
- peptide
- transcriptional activation
- fusion protein
- protein
- host cell
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 7
- 230000027455 binding Effects 0.000 title abstract 4
- 230000003993 interaction Effects 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 6
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 abstract 5
- 108020001507 fusion proteins Proteins 0.000 abstract 4
- 102000037865 fusion proteins Human genes 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 230000004568 DNA-binding Effects 0.000 abstract 2
- 108700008625 Reporter Genes Proteins 0.000 abstract 1
- 239000013611 chromosomal DNA Substances 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Mobile Radio Communication Systems (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
This invention relates to novel modified host cells which express heterologous fused proteins and methods of screening for test samples having peptide-binding activity; wherein the modified host cell comprises: (a) a gene sequence encoding a heterologous fusion protein; said fusion protein comprising a first peptide of a peptide binding pair, or segment of said first peptide, which is joined to either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation protein; (b) a gene sequence encoding a heterologous fusion protein, said fusion protein comprising a second peptide of the peptide binding pair in (a), or a segment thereof, fused to either a DNA binding domain or its corresponding transcriptional activation domain, whichever one is not employed in (a); (c) a reporter gene operatively associated with the transcriptional activation protein, or a portion thereof; (d) optionally, a deletion or mutation in the chromosomal DNA of the host cell for the transcriptional activation protein if present in the selected host cell.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/259,609 US5989808A (en) | 1994-06-14 | 1994-06-14 | Identification of compounds affecting specific interaction of peptide binding pairs |
Publications (1)
Publication Number | Publication Date |
---|---|
IL114118A0 true IL114118A0 (en) | 1995-10-31 |
Family
ID=22985626
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL11411895A IL114118A0 (en) | 1994-06-14 | 1995-06-12 | Novel cell systems having specific interaction of peptide binding pairs |
Country Status (18)
Country | Link |
---|---|
US (3) | US5989808A (en) |
EP (1) | EP0765389B1 (en) |
AT (1) | ATE245189T1 (en) |
AU (1) | AU706173B2 (en) |
CA (1) | CA2195083A1 (en) |
DE (1) | DE69531302T2 (en) |
DK (1) | DK0765389T3 (en) |
ES (1) | ES2202366T3 (en) |
GE (1) | GEP20022619B (en) |
IL (1) | IL114118A0 (en) |
LT (1) | LT4230B (en) |
LV (1) | LV11906B (en) |
NZ (1) | NZ287372A (en) |
PT (1) | PT765389E (en) |
RU (1) | RU2208646C2 (en) |
SI (1) | SI9520073B (en) |
WO (1) | WO1995034646A1 (en) |
ZA (1) | ZA954892B (en) |
Families Citing this family (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7226761B2 (en) * | 1992-11-05 | 2007-06-05 | Danisco Sweeteners Oy | Manufacture of five-carbon sugars and sugar alcohols |
US6891021B2 (en) | 1993-02-12 | 2005-05-10 | Board Of Trustees Of The Leland Stanford Junior University | Regulated apoptosis |
US6972193B1 (en) * | 1993-02-12 | 2005-12-06 | Board Of Trustees Of Leland Stanford Junior University | Regulated transcription of targeted genes and other biological events |
US6770446B1 (en) * | 1994-06-14 | 2004-08-03 | Wyeth | Cell systems having specific interaction of peptide binding pairs |
US5610015A (en) * | 1995-03-23 | 1997-03-11 | Wisconsin Alumni Research Foundation | System to detect protein-RNA interactions |
AU716893B2 (en) * | 1995-04-11 | 2000-03-09 | General Hospital Corporation, The | Reverse two-hybrid systems |
US5837844A (en) * | 1995-06-07 | 1998-11-17 | Biogen, Inc. | CAIP-like gene family |
US6423824B1 (en) | 1995-06-07 | 2002-07-23 | Biogen, Inc. | CAIP-like gene family |
US6171800B1 (en) | 1995-06-07 | 2001-01-09 | Biogen, Inc. | Method of making and binding CAIP polypeptides |
DE29724617U1 (en) * | 1996-04-26 | 2002-06-13 | Massachusetts Institute Of Technology, Cambridge, Mass. | Three hybrid kit for molecular selection |
JP2000508923A (en) * | 1996-04-26 | 2000-07-18 | マサチューセッツ・インスティテュート・オブ・テクノロジー | 3-Hybrid screening assay |
US6696251B1 (en) | 1996-05-31 | 2004-02-24 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
WO1997049808A1 (en) * | 1996-06-27 | 1997-12-31 | Biogen, Inc. | The caip-like gene family |
FR2761697B1 (en) * | 1997-04-03 | 1999-05-28 | Inst Nat Sante Rech Med | METHODS OF DETECTING INTERACTIONS BETWEEN MULTIPLE PROTEINS |
DE19715683C2 (en) * | 1997-04-15 | 1999-11-11 | Max Planck Gesellschaft | New reporter vectors for one-hybrid and two-hybrid systems |
US7005295B1 (en) | 1997-04-16 | 2006-02-28 | Wyeth | β-amyloid peptide-binding proteins and polynucleotides encoding the same |
US6787319B2 (en) | 1997-04-16 | 2004-09-07 | American Home Products Corp. | β-amyloid peptide-binding proteins and polynucleotides encoding the same |
PT977846E (en) | 1997-04-25 | 2002-11-29 | Wyeth Corp | NEURONAL MORTIE ISOFORMS |
US6479653B1 (en) | 1997-08-26 | 2002-11-12 | Ariad Gene Therapeutics, Inc. | Compositions and method for regulation of transcription |
JP4296608B2 (en) * | 1997-08-27 | 2009-07-15 | 田辺三菱製薬株式会社 | PPAR agonist and antagonist screening method |
WO1999019518A1 (en) | 1997-10-15 | 1999-04-22 | Diversa, Inc. | Screening for novel compounds which regulate biological interactions |
EP1149920A1 (en) * | 1997-10-15 | 2001-10-31 | Diversa, Inc. | Screening for novel compounds which regulate biological interactions |
US6261842B1 (en) | 1997-10-23 | 2001-07-17 | Wisconsin Alumni Research Foundation | Microorganism genomics, compositions and methods related thereto |
CA2309763A1 (en) * | 1997-11-14 | 1999-05-27 | Roche Diagnostics Corporation | Variable region fusion peptides that form effector complexes in the presence of antigen |
CA2317816C (en) | 1998-01-09 | 2009-02-17 | Tvw Telethon Institute For Child Health Research | Peptide detection method |
EP0974649A1 (en) * | 1998-06-22 | 2000-01-26 | Universität Zürich | Screening system |
US7166475B2 (en) | 1999-02-26 | 2007-01-23 | Cyclacel Ltd. | Compositions and methods for monitoring the modification state of a pair of polypeptides |
GB9907687D0 (en) * | 1999-04-01 | 1999-05-26 | Kudos Pharm Ltd | Assays methods and means |
WO2000063667A2 (en) | 1999-04-21 | 2000-10-26 | American Home Products Corporation | Methods for identifying modulators of n-type ion channel inactivation |
AU2001257157A1 (en) * | 2000-04-24 | 2001-11-07 | Wyeth | Novel cell systems having specific interaction of peptide binding pairs |
ZA200301579B (en) | 2000-08-25 | 2004-06-21 | Basf Plant Science Gmbh | Plant polynucleotides encoding prenyl proteases. |
WO2002046400A2 (en) * | 2000-12-08 | 2002-06-13 | The Board Of Trustees Of The University Of Illinois | Mutated class ii major histocompatibility proteins |
NZ538464A (en) | 2002-08-21 | 2008-01-31 | Revivicor Inc | Porcine animals lacking any expression of functional alpha 1,3 galactosyltransferase |
US9453251B2 (en) | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
US7285269B2 (en) | 2002-12-02 | 2007-10-23 | Amgen Fremont, Inc. | Antibodies directed to tumor necrosis factor |
WO2005049804A2 (en) * | 2003-11-17 | 2005-06-02 | Georgia Tech Research Corporation | Engineering enzymes through genetic selection |
EP1544307A1 (en) * | 2003-12-18 | 2005-06-22 | AXXAM S.r.l. | LAC9 chimeric receptor and uses thereof |
EP1774017B1 (en) | 2004-07-26 | 2013-05-15 | Pfenex Inc. | Process for improved protein expression by strain engineering |
WO2007035213A2 (en) | 2005-08-09 | 2007-03-29 | Revivicor, Inc. | Transgenic ungulates expressing ctla4-ig and uses thereof |
US20070196872A1 (en) * | 2006-02-07 | 2007-08-23 | Wyeth | Materials and methods for identifying agents that modulate Norrin, Norrin mimetics, and agents identified thereby |
US9580719B2 (en) | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
CA2685326A1 (en) | 2007-04-27 | 2008-11-06 | Dow Global Technologies Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
CN101131363B (en) * | 2007-09-30 | 2010-12-01 | 南京大学 | Yeast triple-hybridization based small molecule compound detecting method |
WO2016154452A2 (en) | 2015-03-24 | 2016-09-29 | Osiris Therapeutics, Inc. | Compositions comprising meniscal tissues and uses thereof |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5283173A (en) * | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
US5322801A (en) * | 1990-04-19 | 1994-06-21 | The General Hospital Corporation | Protein partner screening assays and uses thereof |
JP3537141B2 (en) * | 1992-10-30 | 2004-06-14 | ザ ゼネラル ホスピタル コーポレーション | Interaction-based capture system for separation of new proteins |
US5512473A (en) * | 1993-01-29 | 1996-04-30 | Brent; Roger | Max-interacting proteins and related molecules and methods |
US5582995A (en) * | 1993-06-11 | 1996-12-10 | The General Hospital Corporation | Methods of screening for compounds which inhibit the direct binding of Ras to Raf |
WO1995018380A1 (en) * | 1993-12-30 | 1995-07-06 | The Salk Institute For Biological Studies | Novel uses for gal4-receptor constructs |
ATE190984T1 (en) * | 1994-01-21 | 2000-04-15 | Icos Corp | MATERIALS AND METHODS RELATING TO CASEIN KINASE I INTERACTING PROTEINS |
US5525490A (en) * | 1994-03-29 | 1996-06-11 | Onyx Pharmaceuticals, Inc. | Reverse two-hybrid method |
US5691147A (en) * | 1994-06-02 | 1997-11-25 | Mitotix, Inc. | CDK4 binding assay |
-
1994
- 1994-06-14 US US08/259,609 patent/US5989808A/en not_active Expired - Fee Related
-
1995
- 1995-05-31 WO PCT/US1995/006895 patent/WO1995034646A1/en active IP Right Grant
- 1995-05-31 EP EP95920689A patent/EP0765389B1/en not_active Expired - Lifetime
- 1995-05-31 DK DK95920689T patent/DK0765389T3/en active
- 1995-05-31 NZ NZ287372A patent/NZ287372A/en not_active IP Right Cessation
- 1995-05-31 DE DE69531302T patent/DE69531302T2/en not_active Expired - Lifetime
- 1995-05-31 SI SI9520073A patent/SI9520073B/en not_active IP Right Cessation
- 1995-05-31 RU RU97100776/13A patent/RU2208646C2/en not_active IP Right Cessation
- 1995-05-31 ES ES95920689T patent/ES2202366T3/en not_active Expired - Lifetime
- 1995-05-31 AT AT95920689T patent/ATE245189T1/en not_active IP Right Cessation
- 1995-05-31 AU AU26066/95A patent/AU706173B2/en not_active Ceased
- 1995-05-31 GE GEAP19953531A patent/GEP20022619B/en unknown
- 1995-05-31 PT PT95920689T patent/PT765389E/en unknown
- 1995-05-31 CA CA002195083A patent/CA2195083A1/en not_active Abandoned
- 1995-06-12 IL IL11411895A patent/IL114118A0/en not_active IP Right Cessation
- 1995-06-13 ZA ZA954892A patent/ZA954892B/en unknown
-
1997
- 1997-01-13 LT LT97-004A patent/LT4230B/en not_active IP Right Cessation
- 1997-02-14 LV LVP-97-04A patent/LV11906B/en unknown
-
1999
- 1999-03-08 US US09/263,944 patent/US6251602B1/en not_active Expired - Fee Related
- 1999-05-06 US US09/305,483 patent/US6284519B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US6251602B1 (en) | 2001-06-26 |
LV11906B (en) | 1998-06-20 |
GEP20022619B (en) | 2002-01-25 |
NZ287372A (en) | 1997-11-24 |
ES2202366T3 (en) | 2004-04-01 |
PT765389E (en) | 2003-11-28 |
US5989808A (en) | 1999-11-23 |
CA2195083A1 (en) | 1995-12-21 |
DE69531302T2 (en) | 2004-04-22 |
ATE245189T1 (en) | 2003-08-15 |
LT4230B (en) | 1997-10-27 |
AU706173B2 (en) | 1999-06-10 |
AU2606695A (en) | 1996-01-05 |
EP0765389A1 (en) | 1997-04-02 |
DK0765389T3 (en) | 2003-10-27 |
RU2208646C2 (en) | 2003-07-20 |
EP0765389B1 (en) | 2003-07-16 |
ZA954892B (en) | 1996-01-30 |
LV11906A (en) | 1997-12-20 |
LT97004A (en) | 1997-07-25 |
WO1995034646A1 (en) | 1995-12-21 |
SI9520073A (en) | 1998-02-28 |
US6284519B1 (en) | 2001-09-04 |
DE69531302D1 (en) | 2003-08-21 |
SI9520073B (en) | 2004-06-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
LT97004A (en) | Novel cell systems having specific interaction of peptide binding pairs | |
ES8602119A1 (en) | Recombinant immunoglobulin preparations, methods for their preparation, DNA sequences, expression vectors and recombinant host cells therefor. | |
EP1559776A3 (en) | Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system and promoters and terminators for use therein | |
DK600486D0 (en) | IgG binding PROTEIN | |
ATE238550T1 (en) | MARKING AND SELECTION OF MOLECULES | |
DE69535147D1 (en) | OVEREXPRESSION OF MAMMAL AND VIRUS PROTEINS | |
DK61595A (en) | Recombinant DNA molecules, host cell transformation thereof, host cells containing them, and method for producing an IFN alpha type polypeptide | |
HK1013847A1 (en) | Gdf-1 and uog-1 proteins | |
WO1999043808A8 (en) | Human c-maf compositions and methods of use thereof | |
ATE311443T1 (en) | METHODS FOR SCREENING PROTEINS | |
WO2000009730A3 (en) | Cell type specific gene transfer using retroviral vectors containing antibody-envelope fusion proteins and wild-type envelope fusion proteins | |
AU3217100A (en) | Methods of use of a novel lysyl oxidase-related protein | |
WO1995014778A3 (en) | Dna sequence coding for a bmp receptor | |
WO1994026891A3 (en) | Purified mammalian flt3 ligands and agonists and antagonists thereof | |
Board | Genetic heterogeneity of the B subunit of coagulation factor XIII: resolution of type 2 | |
WO2001094383A3 (en) | Mutated furin polypeptides having improved characteristics | |
WO2000012544A3 (en) | Novel irap-bp polypeptide and nucleic acid molecules and uses therefor | |
DK0750637T3 (en) | CAI resistance encoding a protein and its use | |
ATE346919T1 (en) | Epididymis-SPECIFIC RECEPTOR PROTEIN AND USE THEREOF | |
WO2002050104A3 (en) | Methods and cells for detecting modulators of rgs proteins | |
WO1999047673A3 (en) | Isolated mammalian membrane protein genes and related reagents | |
NO20012471L (en) | Potassium channel interactors and uses thereof | |
WO2000027874A3 (en) | Nucleic acids encoding transferrin receptor-like proteins and products related thereto | |
EA200300420A1 (en) | Isolated nucleic acid molecules, isolated polypeptides AND METHOD FOR PRODUCTION THEREOF, METHOD FOR DETECTING THEM present in the sample (VARIANTS) host cell (VARIANTS) antibody kit (OPTIONS), a method of modulating activity of a polypeptide, IDENTIFICATION METHOD (VARIANTS) AND APPLICATION OF COMPOUNDS METHOD OF IDENTIFICATION OF THE SUBJECT (OPTIONS) AND METHOD OF TREATING THE SUBJECT WITH DAMAGE ASSOCIATED WITH POTASSIUM CHANNELS (OPTIONS) | |
EP0688224A4 (en) | A mutant protein and methods and materials for making and using it |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FF | Patent granted | ||
KB | Patent renewed | ||
KB | Patent renewed | ||
KB | Patent renewed | ||
MM9K | Patent not in force due to non-payment of renewal fees |