IE67035B1 - Production and use of non-glycosylated IL-6 - Google Patents
Production and use of non-glycosylated IL-6Info
- Publication number
- IE67035B1 IE67035B1 IE172687A IE172687A IE67035B1 IE 67035 B1 IE67035 B1 IE 67035B1 IE 172687 A IE172687 A IE 172687A IE 172687 A IE172687 A IE 172687A IE 67035 B1 IE67035 B1 IE 67035B1
- Authority
- IE
- Ireland
- Prior art keywords
- polypeptide
- sequence
- expression
- pharmaceutical composition
- dna sequence
- Prior art date
Links
- 108090001005 Interleukin-6 Proteins 0.000 title claims abstract description 92
- 238000004519 manufacturing process Methods 0.000 title description 12
- 238000000034 method Methods 0.000 claims abstract description 25
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 25
- 238000011282 treatment Methods 0.000 claims abstract description 17
- 102000004889 Interleukin-6 Human genes 0.000 claims description 87
- 210000004027 cell Anatomy 0.000 claims description 40
- 230000001580 bacterial effect Effects 0.000 claims description 33
- 108090000623 proteins and genes Proteins 0.000 claims description 30
- 102000004169 proteins and genes Human genes 0.000 claims description 27
- 239000013598 vector Substances 0.000 claims description 24
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 23
- 230000000694 effects Effects 0.000 claims description 22
- 229920001184 polypeptide Polymers 0.000 claims description 18
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 18
- 238000003556 assay Methods 0.000 claims description 16
- 210000001185 bone marrow Anatomy 0.000 claims description 12
- 108010002350 Interleukin-2 Proteins 0.000 claims description 11
- 108010002386 Interleukin-3 Proteins 0.000 claims description 8
- 239000008194 pharmaceutical composition Substances 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 230000028327 secretion Effects 0.000 claims description 7
- 230000004048 modification Effects 0.000 claims description 6
- 238000012986 modification Methods 0.000 claims description 6
- 102000008070 Interferon-gamma Human genes 0.000 claims description 5
- 108010074328 Interferon-gamma Proteins 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 229940044627 gamma-interferon Drugs 0.000 claims description 5
- 239000003102 growth factor Substances 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 241001529936 Murinae Species 0.000 claims description 4
- 210000004698 lymphocyte Anatomy 0.000 claims description 4
- 230000003248 secreting effect Effects 0.000 claims description 4
- 102000015696 Interleukins Human genes 0.000 claims description 3
- 108010063738 Interleukins Proteins 0.000 claims description 3
- 206010028980 Neoplasm Diseases 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 210000001322 periplasm Anatomy 0.000 claims description 3
- 208000036142 Viral infection Diseases 0.000 claims description 2
- 201000011510 cancer Diseases 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims description 2
- 230000002068 genetic effect Effects 0.000 claims description 2
- 230000035772 mutation Effects 0.000 claims description 2
- 210000000066 myeloid cell Anatomy 0.000 claims description 2
- 230000009385 viral infection Effects 0.000 claims description 2
- 102000012428 Hematopoietic Cell Growth Factors Human genes 0.000 abstract description 9
- 108010022580 Hematopoietic Cell Growth Factors Proteins 0.000 abstract description 9
- 210000003958 hematopoietic stem cell Anatomy 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 230000007812 deficiency Effects 0.000 abstract description 2
- 238000011275 oncology therapy Methods 0.000 abstract description 2
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 208000035475 disorder Diseases 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 description 26
- 235000018102 proteins Nutrition 0.000 description 26
- 102000004594 DNA Polymerase I Human genes 0.000 description 13
- 108010017826 DNA Polymerase I Proteins 0.000 description 13
- 125000003729 nucleotide group Chemical group 0.000 description 11
- 239000012634 fragment Substances 0.000 description 10
- 239000002773 nucleotide Substances 0.000 description 10
- 108091026890 Coding region Proteins 0.000 description 9
- 102000000588 Interleukin-2 Human genes 0.000 description 9
- 235000001014 amino acid Nutrition 0.000 description 9
- 239000002299 complementary DNA Substances 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 230000001225 therapeutic effect Effects 0.000 description 9
- 229940024606 amino acid Drugs 0.000 description 8
- 150000001413 amino acids Chemical class 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 7
- 102000000646 Interleukin-3 Human genes 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- 230000029087 digestion Effects 0.000 description 5
- 239000013604 expression vector Substances 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 102000007644 Colony-Stimulating Factors Human genes 0.000 description 4
- 108010071942 Colony-Stimulating Factors Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 108090000144 Human Proteins Proteins 0.000 description 4
- 102000003839 Human Proteins Human genes 0.000 description 4
- 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 4
- 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 4
- 235000004279 alanine Nutrition 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 229940047120 colony stimulating factors Drugs 0.000 description 4
- 239000003636 conditioned culture medium Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 108020004705 Codon Proteins 0.000 description 3
- 108010002616 Interleukin-5 Proteins 0.000 description 3
- 102000000743 Interleukin-5 Human genes 0.000 description 3
- 101710163270 Nuclease Proteins 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 238000011260 co-administration Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 108700028369 Alleles Proteins 0.000 description 2
- 101100353517 Caenorhabditis elegans pas-2 gene Proteins 0.000 description 2
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 2
- 241001646716 Escherichia coli K-12 Species 0.000 description 2
- 241000701959 Escherichia virus Lambda Species 0.000 description 2
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 2
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 2
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 2
- 102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 description 2
- 101001076408 Homo sapiens Interleukin-6 Proteins 0.000 description 2
- 206010061598 Immunodeficiency Diseases 0.000 description 2
- 208000029462 Immunodeficiency disease Diseases 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 210000002798 bone marrow cell Anatomy 0.000 description 2
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000013613 expression plasmid Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 230000013595 glycosylation Effects 0.000 description 2
- 238000006206 glycosylation reaction Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 230000007813 immunodeficiency Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 201000002364 leukopenia Diseases 0.000 description 2
- 231100001022 leukopenia Toxicity 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 229940124597 therapeutic agent Drugs 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 101100269496 Alcaligenes faecalis aioA gene Proteins 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 101100290380 Caenorhabditis elegans cel-1 gene Proteins 0.000 description 1
- 108091033380 Coding strand Proteins 0.000 description 1
- 241000143940 Colias Species 0.000 description 1
- 102100024746 Dihydrofolate reductase Human genes 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000003951 Erythropoietin Human genes 0.000 description 1
- 108090000394 Erythropoietin Proteins 0.000 description 1
- 206010073306 Exposure to radiation Diseases 0.000 description 1
- GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 1
- 241000598436 Human T-cell lymphotropic virus Species 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000000589 Interleukin-1 Human genes 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- 102000004125 Interleukin-1alpha Human genes 0.000 description 1
- 108010082786 Interleukin-1alpha Proteins 0.000 description 1
- 102100039064 Interleukin-3 Human genes 0.000 description 1
- 102100026019 Interleukin-6 Human genes 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- -1 Meg-CSF Proteins 0.000 description 1
- 101001033276 Mus musculus Interleukin-3 Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 230000024932 T cell mediated immunity Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000003969 blast cell Anatomy 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 101150070926 ct gene Proteins 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 108020001096 dihydrofolate reductase Proteins 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 229940105423 erythropoietin Drugs 0.000 description 1
- 238000001400 expression cloning Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 229960002949 fluorouracil Drugs 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002489 hematologic effect Effects 0.000 description 1
- 210000000777 hematopoietic system Anatomy 0.000 description 1
- 230000002607 hemopoietic effect Effects 0.000 description 1
- 102000052611 human IL6 Human genes 0.000 description 1
- 230000028996 humoral immune response Effects 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 230000003832 immune regulation Effects 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 101150018339 lacS gene Proteins 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 208000037819 metastatic cancer Diseases 0.000 description 1
- 208000011575 metastatic malignant neoplasm Diseases 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229940075461 other therapeutic product in atc Drugs 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 229940038237 tumor antigen vaccine Drugs 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5412—IL-6
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
- A61K38/20—Interleukins [IL]
- A61K38/2013—IL-2
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
- A61K38/20—Interleukins [IL]
- A61K38/204—IL-6
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
- A61K38/21—Interferons [IFN]
- A61K38/217—IFN-gamma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Epidemiology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Saccharide Compounds (AREA)
- Steroid Compounds (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US88320786A | 1986-07-08 | 1986-07-08 | |
US88590586A | 1986-07-15 | 1986-07-15 | |
US4795787A | 1987-05-08 | 1987-05-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
IE871726L IE871726L (en) | 1988-01-08 |
IE67035B1 true IE67035B1 (en) | 1996-02-21 |
Family
ID=27367237
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IE172687A IE67035B1 (en) | 1986-07-08 | 1987-06-26 | Production and use of non-glycosylated IL-6 |
Country Status (18)
Country | Link |
---|---|
EP (2) | EP0574955B1 (de) |
JP (1) | JP2664698B2 (de) |
KR (1) | KR960013601B1 (de) |
AT (2) | ATE124959T1 (de) |
AU (1) | AU615630B2 (de) |
CA (1) | CA1341355C (de) |
DE (2) | DE3751922T2 (de) |
DK (1) | DK173279B1 (de) |
ES (1) | ES2006776A6 (de) |
GR (1) | GR871027B (de) |
IE (1) | IE67035B1 (de) |
IL (1) | IL83137A (de) |
MY (1) | MY102865A (de) |
NO (1) | NO176665C (de) |
PL (1) | PL159182B1 (de) |
PT (1) | PT85285B (de) |
SK (1) | SK279144B6 (de) |
WO (1) | WO1988000206A1 (de) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5554513A (en) * | 1979-11-21 | 1996-09-10 | Yeda Research & Development Co. Ltd. | Production of recombinant human interferon-beta2 |
US5510472A (en) * | 1979-11-21 | 1996-04-23 | Yeda Research And Development Co. Ltd. | Production of recombinant human interferon-beta2 |
DE3688671T3 (de) * | 1985-10-14 | 2001-05-03 | Yeda Research And Development Co., Ltd. | Menschliches Interferon-beta2A ; Vektoren, die Gene enthalten, die für das genannte Interferon kodieren; Zellinien, die dieses produzieren und Verwendung des genannten Interferons als Pharmazeutikum. |
JP2648860B2 (ja) * | 1986-05-08 | 1997-09-03 | イエダ リサーチ アンド デベロップメント コンパニー リミテッド | ヒトインターフエロン−β2A及びヒトインターフエロン−β2B、該インターフエロンをコードする遺伝子を含むベクター、該インターフエロンを産生するセルライン及び該インターフエロンの医薬品としての用途 |
JP2676336B2 (ja) * | 1986-08-06 | 1997-11-12 | 忠三 岸本 | ヒトb細胞分化因子の遺伝子 |
JPS6356291A (ja) * | 1986-08-27 | 1988-03-10 | Chuzo Kishimoto | リコンビナントbcdf |
EP0257406B2 (de) * | 1986-08-06 | 1998-08-12 | Ajinomoto Co., Inc. | Rekombinanter B-Zell-Differenzierungsfaktor |
NZ226799A (en) * | 1987-11-06 | 1991-08-27 | Oncogen | Breast cancer inhibitory factor and method for inhibiting proliferation of neoplastic cells and compositions therefor |
IL85204A0 (en) * | 1988-01-26 | 1988-07-31 | Yeda Res & Dev | Recombinant human interferon beta2,its preparation and pharmaceutical compositions comprising it |
CA1341588C (en) * | 1988-01-26 | 2009-01-06 | Michel Revel | Human ifn-beta2/i1-6, its purification and use |
HUT52962A (en) * | 1988-03-04 | 1990-09-28 | Sandoz Ag | Process for production of medical compositions eliminating bone-absorption and promoting forming of bones |
JPH022353A (ja) * | 1988-03-10 | 1990-01-08 | Tosoh Corp | ヒトb細胞分化因子の製造法 |
JPH022354A (ja) * | 1988-03-31 | 1990-01-08 | Tosoh Corp | ヒトb細胞分化因子の製造法 |
GB2217327B (en) * | 1988-04-12 | 1992-01-29 | British Bio Technology | Synthetic interleukin-6 gene |
US5223407A (en) * | 1988-08-31 | 1993-06-29 | Allelix Inc. | Excretion of heterologous proteins from e. coli |
US5646015A (en) * | 1988-08-31 | 1997-07-08 | Astra Ab | Excretion of heterologous proteins from E. coli |
US5271931A (en) * | 1988-09-14 | 1993-12-21 | The Scripps Research Institute | Methods for increasing C1 inhibitor concentrations using interferon-gamma and/or interleukin-6 |
JPH0372429A (ja) * | 1988-10-07 | 1991-03-27 | Chugai Pharmaceut Co Ltd | 血小板減少症の治療剤 |
US5811523A (en) | 1988-11-10 | 1998-09-22 | Trinchieri; Giorgio | Antibodies to natural killer stimulatory factor |
JP2805224B2 (ja) * | 1989-01-13 | 1998-09-30 | 味の素株式会社 | 血小板減少症治療剤 |
JPH0331215A (ja) * | 1989-06-27 | 1991-02-12 | Ajinomoto Co Inc | 制癌療法支持剤 |
DE3939706C1 (de) * | 1989-12-01 | 1991-03-21 | Centre Regional De Transfusion Sanguine, Besancon, Fr | |
JPH04218000A (ja) * | 1990-02-13 | 1992-08-07 | Kirin Amgen Inc | 修飾ポリペプチド |
DE69122764T2 (de) * | 1990-08-31 | 1997-03-20 | Teruo Nishida | Arznei gegen hornhautbeschädigungen |
US5641868A (en) * | 1991-04-18 | 1997-06-24 | Toray Industries, Inc. | Interlekin-6 compositions, and a production process thereof |
ES2164050T3 (es) * | 1991-04-18 | 2002-02-16 | Toray Industries | Preparacion de compuestos de interleuquina-6. |
ZA924674B (en) * | 1991-07-02 | 1993-04-28 | Imclone Systems Inc | Cysteine depleted il-6 mutein |
US5338833A (en) * | 1992-07-23 | 1994-08-16 | The University Of North Carolina At Chapel Hill | Carboxy terminal IL-6 muteins |
US5338834A (en) * | 1993-01-26 | 1994-08-16 | Allelix Biopharmaceuticals Inc. | Ultrapure human interleukin-6 |
WO1994028919A1 (en) * | 1993-06-08 | 1994-12-22 | Ajinomoto Co., Inc. | Hematopoietic cell proliferation accelerator |
US5578301A (en) * | 1993-12-14 | 1996-11-26 | Sandoz Ltd. | Method for using GM-CSF to reduce the acute phase response in a patient being administered IL-6 therapy |
US5599536A (en) * | 1993-12-13 | 1997-02-04 | Sandoz Ltd. | Method for suppressing the acute phase response in a patient receiving IL-6 therapy |
US5641657A (en) * | 1994-05-19 | 1997-06-24 | Human Genome Sciences, Inc. | DNA encoding an interleukin-6 splice variant |
EP0721780A3 (de) * | 1994-12-16 | 1997-12-17 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Mittel zur Förderung von Plättchen und/oder Leucozytenherstellung |
WO1998003657A1 (en) * | 1996-07-19 | 1998-01-29 | Dade Behring Marburg Gmbh | Viral interleukin-6 |
SE9702401D0 (sv) | 1997-06-19 | 1997-06-19 | Astra Ab | Pharmaceutical use |
US7479540B1 (en) | 2003-12-22 | 2009-01-20 | Chandan Prasad | Adipomodulin and related molecules and methods |
US20090136444A1 (en) * | 2005-02-15 | 2009-05-28 | Apollo Life Sciences Limited | molecule and chimeric molecules thereof |
ES2302402B1 (es) * | 2005-06-16 | 2009-05-08 | Proyecto De Biomedicina Cima, S.L. | Uso de una citoquina de la familia de interleuquina-6 en la preparacion de una composicion para administracion combinada con interferon-alfa. |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL58765A (en) * | 1979-11-21 | 1986-09-30 | Yeda Res & Dev | Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta |
US4675285A (en) * | 1984-09-19 | 1987-06-23 | Genetics Institute, Inc. | Method for identification and isolation of DNA encoding a desired protein |
DE3688671T3 (de) * | 1985-10-14 | 2001-05-03 | Yeda Research And Development Co., Ltd. | Menschliches Interferon-beta2A ; Vektoren, die Gene enthalten, die für das genannte Interferon kodieren; Zellinien, die dieses produzieren und Verwendung des genannten Interferons als Pharmazeutikum. |
EP0257406B2 (de) * | 1986-08-06 | 1998-08-12 | Ajinomoto Co., Inc. | Rekombinanter B-Zell-Differenzierungsfaktor |
-
1987
- 1987-06-26 IE IE172687A patent/IE67035B1/en not_active IP Right Cessation
- 1987-06-30 GR GR871027A patent/GR871027B/el unknown
- 1987-07-03 CA CA000541216A patent/CA1341355C/en not_active Expired - Fee Related
- 1987-07-07 MY MYPI87000953A patent/MY102865A/en unknown
- 1987-07-07 SK SK5150-87A patent/SK279144B6/sk unknown
- 1987-07-07 PL PL1987266680A patent/PL159182B1/pl unknown
- 1987-07-07 DE DE3751922T patent/DE3751922T2/de not_active Expired - Fee Related
- 1987-07-07 WO PCT/US1987/001611 patent/WO1988000206A1/en not_active Application Discontinuation
- 1987-07-07 AU AU76962/87A patent/AU615630B2/en not_active Ceased
- 1987-07-07 EP EP93110770A patent/EP0574955B1/de not_active Expired - Lifetime
- 1987-07-07 AT AT87904625T patent/ATE124959T1/de not_active IP Right Cessation
- 1987-07-07 DE DE3751406T patent/DE3751406T2/de not_active Revoked
- 1987-07-07 ES ES8701985A patent/ES2006776A6/es not_active Expired
- 1987-07-07 KR KR1019880700256A patent/KR960013601B1/ko not_active IP Right Cessation
- 1987-07-07 AT AT93110770T patent/ATE143811T1/de not_active IP Right Cessation
- 1987-07-07 EP EP87904625A patent/EP0316319B1/de not_active Revoked
- 1987-07-07 JP JP62504291A patent/JP2664698B2/ja not_active Expired - Fee Related
- 1987-07-08 PT PT85285A patent/PT85285B/pt active IP Right Revival
- 1987-07-09 IL IL8313787A patent/IL83137A/en not_active IP Right Cessation
-
1988
- 1988-03-02 NO NO880929A patent/NO176665C/no unknown
- 1988-03-07 DK DK198801217A patent/DK173279B1/da not_active IP Right Cessation
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0316319B1 (de) | Herstellung und Verwendung von nicht-glycosiliertem IL-6 | |
US5573763A (en) | Family of CSF-l proteins | |
US4868119A (en) | Hematopoietic growth factors | |
US6066317A (en) | Method of using IL-11 for treating deficiencies in hematopoietic progenitor or stem cells | |
AU637620B2 (en) | A human cytokine, interleukin-9 | |
PH26757A (en) | Recombinant DNA encoding novel family of primate hematop-poietic growth factors | |
US6348191B1 (en) | Production and use of IL-6 | |
US6284237B1 (en) | Methods of treatment using IL-6 | |
AU644389C (en) | A mammalian cytokine, IL-11 | |
IE70605B1 (en) | A novel family of primate hematopoietic growth factors |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM4A | Patent lapsed |