ID26107A - Metoda untuk amplifikasi tak spesifik dari asam nukleat - Google Patents
Metoda untuk amplifikasi tak spesifik dari asam nukleatInfo
- Publication number
- ID26107A ID26107A IDW20001643A ID20001643A ID26107A ID 26107 A ID26107 A ID 26107A ID W20001643 A IDW20001643 A ID W20001643A ID 20001643 A ID20001643 A ID 20001643A ID 26107 A ID26107 A ID 26107A
- Authority
- ID
- Indonesia
- Prior art keywords
- rna
- sequence
- enzyme
- mrna
- rna polymerase
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 9
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 5
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 abstract 4
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 4
- 108090000790 Enzymes Proteins 0.000 abstract 4
- 230000000694 effects Effects 0.000 abstract 4
- 108020004999 messenger RNA Proteins 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 2
- 102100034343 Integrase Human genes 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 239000011541 reaction mixture Substances 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 1
- 101710203526 Integrase Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 101710086015 RNA ligase Proteins 0.000 abstract 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 1
- 238000010804 cDNA synthesis Methods 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
- 230000005026 transcription initiation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Steroid Compounds (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98200619 | 1998-02-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
ID26107A true ID26107A (id) | 2000-11-23 |
Family
ID=8233425
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IDW20001643A ID26107A (id) | 1998-02-27 | 1999-01-21 | Metoda untuk amplifikasi tak spesifik dari asam nukleat |
Country Status (14)
Country | Link |
---|---|
US (3) | US6338954B1 (fr) |
EP (2) | EP1056884B9 (fr) |
JP (1) | JP3641206B2 (fr) |
KR (1) | KR100440044B1 (fr) |
AT (1) | ATE210736T1 (fr) |
AU (1) | AU748847B2 (fr) |
CA (1) | CA2322073C (fr) |
DE (1) | DE69900592T2 (fr) |
DK (1) | DK1056884T3 (fr) |
ES (1) | ES2169943T3 (fr) |
ID (1) | ID26107A (fr) |
PT (1) | PT1056884E (fr) |
WO (1) | WO1999043850A1 (fr) |
ZA (1) | ZA99617B (fr) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6582938B1 (en) * | 2001-05-11 | 2003-06-24 | Affymetrix, Inc. | Amplification of nucleic acids |
US6794138B1 (en) * | 1999-12-16 | 2004-09-21 | Affymetrix, Inc. | Methods of small sample amplification |
WO2001064959A1 (fr) * | 2000-03-02 | 2001-09-07 | Akzo Nobel N.V. | Detection d'arn du virus de l'hepatite b |
US9261460B2 (en) * | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
US7727722B2 (en) * | 2001-08-29 | 2010-06-01 | General Electric Company | Ligation amplification |
US7166478B2 (en) * | 2002-03-12 | 2007-01-23 | Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. | Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses |
US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
CA2501983A1 (fr) * | 2002-10-30 | 2004-05-27 | Pamgene B.V. | Procedes ameliores permettant de generer de multiples copies d'arn |
US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
US6852494B2 (en) | 2003-01-10 | 2005-02-08 | Linden Technologies, Inc. | Nucleic acid amplification |
AU2004239283B2 (en) * | 2003-05-09 | 2010-01-28 | Genisphere, Llc | Methods for amplification of nucleic acid sequences using staggered ligation |
US20050153333A1 (en) * | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
CN1985006B (zh) | 2004-04-01 | 2010-12-08 | 杰尼斯菲尔股份有限公司 | 用启动子模板扩增核苷序列的方法 |
FR2876116B1 (fr) * | 2004-10-01 | 2007-04-06 | Biomerieux Sa | Methode pour generer des transcrits |
EP1828422A4 (fr) * | 2004-11-15 | 2008-07-30 | Bacterial Barcodes Inc | Detection au moyen d'amorces vers l'adn repetitif et amplification basee sur la transcription par une telle detection |
AU2005322131B2 (en) * | 2004-12-23 | 2011-11-10 | Global Life Sciences Solutions Usa Llc | Ligation-based RNA amplification |
AU2012200749B2 (en) * | 2004-12-23 | 2012-10-04 | Global Life Sciences Solutions Usa Llc | Ligation-based RNA amplification |
US8309303B2 (en) | 2005-04-01 | 2012-11-13 | Qiagen Gmbh | Reverse transcription and amplification of RNA with simultaneous degradation of DNA |
EP1762627A1 (fr) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Procédé pour l'activation d'acides nucléiques pour effectuer une réaction d'une polymérase |
DE102006038113A1 (de) | 2006-08-14 | 2008-02-21 | Qiagen Gmbh | Verfahren zur Synthese einer cDNA in einer Probe in einer enzymatischen Reaktion und gleichzeitigen Bereitstellung eines ersten Enzyms mit Polyadenylierungsaktivität und eines zweiten Enzyms mit reverser Transkriptaseaktivität |
JP5409631B2 (ja) * | 2007-08-13 | 2014-02-05 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 酵素反応においてサンプル中でcDNAを合成するための方法 |
US8951731B2 (en) * | 2007-10-15 | 2015-02-10 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
US8703421B2 (en) | 2008-05-30 | 2014-04-22 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of Salmonella |
EP2280080A1 (fr) | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Procédé de quantification normalisée d'acides nucléiques utilisant des oligonucléotides d'ancrage et oligonucléotides d'adaptateur |
EP2280081A1 (fr) * | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Procédé de quantification normalisée d'ARN |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4661450A (en) * | 1983-05-03 | 1987-04-28 | Molecular Genetics Research And Development Limited Partnership | Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides |
US5472850A (en) | 1991-04-10 | 1995-12-05 | Oklahoma Medical Research Foundation | Quantitative clotting assay for activated factor VII |
EP0731175A3 (fr) * | 1989-07-11 | 2004-05-26 | Gen-Probe Incorporated | Sondes oligonucléotidiques pour d'acide nucléique de VIH |
US5166057A (en) * | 1989-08-28 | 1992-11-24 | The Mount Sinai School Of Medicine Of The City University Of New York | Recombinant negative strand rna virus expression-systems |
US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
WO1993022461A1 (fr) * | 1992-05-06 | 1993-11-11 | Gen-Probe Incorporated | Procede, composition et kit pour l'amplification de sequences d'acides nucleiques |
US5514545A (en) * | 1992-06-11 | 1996-05-07 | Trustees Of The University Of Pennsylvania | Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics |
PT776376E (pt) * | 1994-07-15 | 2002-05-31 | Organon Teknika Bv | Utilizacao de arn-polimerase para melhorar o processo de amplificacao de acido nucleico |
FR2724934B1 (fr) * | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
AU2253397A (en) | 1996-01-23 | 1997-08-20 | Affymetrix, Inc. | Nucleic acid analysis techniques |
US6114152A (en) * | 1997-12-12 | 2000-09-05 | The Regents Of The University Of California | Methods for making nucleic acids |
-
1999
- 1999-01-21 PT PT99904801T patent/PT1056884E/pt unknown
- 1999-01-21 EP EP99904801A patent/EP1056884B9/fr not_active Expired - Lifetime
- 1999-01-21 AU AU25189/99A patent/AU748847B2/en not_active Ceased
- 1999-01-21 ES ES99904801T patent/ES2169943T3/es not_active Expired - Lifetime
- 1999-01-21 EP EP01121247A patent/EP1170381A3/fr not_active Withdrawn
- 1999-01-21 AT AT99904801T patent/ATE210736T1/de not_active IP Right Cessation
- 1999-01-21 KR KR10-2000-7009493A patent/KR100440044B1/ko not_active IP Right Cessation
- 1999-01-21 ID IDW20001643A patent/ID26107A/id unknown
- 1999-01-21 US US09/623,054 patent/US6338954B1/en not_active Expired - Fee Related
- 1999-01-21 JP JP2000533589A patent/JP3641206B2/ja not_active Expired - Fee Related
- 1999-01-21 DK DK99904801T patent/DK1056884T3/da active
- 1999-01-21 WO PCT/EP1999/000332 patent/WO1999043850A1/fr active IP Right Grant
- 1999-01-21 DE DE69900592T patent/DE69900592T2/de not_active Expired - Fee Related
- 1999-01-21 CA CA002322073A patent/CA2322073C/fr not_active Expired - Fee Related
- 1999-01-27 ZA ZA9900617A patent/ZA99617B/xx unknown
-
2001
- 2001-11-01 US US09/985,024 patent/US20020061533A1/en not_active Abandoned
-
2002
- 2002-12-11 US US10/316,456 patent/US20030113875A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO1999043850A1 (fr) | 1999-09-02 |
EP1056884A1 (fr) | 2000-12-06 |
US20020061533A1 (en) | 2002-05-23 |
PT1056884E (pt) | 2002-06-28 |
US20030113875A1 (en) | 2003-06-19 |
CA2322073C (fr) | 2003-12-23 |
EP1056884B9 (fr) | 2002-07-24 |
CA2322073A1 (fr) | 1999-09-02 |
KR20010041371A (ko) | 2001-05-15 |
EP1170381A3 (fr) | 2004-01-28 |
ES2169943T3 (es) | 2002-07-16 |
JP2002505087A (ja) | 2002-02-19 |
AU2518999A (en) | 1999-09-15 |
ZA99617B (en) | 1999-07-27 |
ATE210736T1 (de) | 2001-12-15 |
US6338954B1 (en) | 2002-01-15 |
JP3641206B2 (ja) | 2005-04-20 |
DE69900592D1 (de) | 2002-01-24 |
EP1056884B1 (fr) | 2001-12-12 |
KR100440044B1 (ko) | 2004-07-14 |
EP1170381A2 (fr) | 2002-01-09 |
AU748847B2 (en) | 2002-06-13 |
DE69900592T2 (de) | 2002-08-22 |
DK1056884T3 (da) | 2002-02-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ID26107A (id) | Metoda untuk amplifikasi tak spesifik dari asam nukleat | |
KR910700335A (ko) | 자속된(self-sustained), 서열 복제 시스템 | |
KR890013184A (ko) | 핵산을 증폭시키는 방법 | |
WO2007149789A2 (fr) | Conversion d'amplification spécifique à une cible en séquençage universel | |
KR900018376A (ko) | 리보핵산(rna)의 합성방법 | |
CA2610929A1 (fr) | Methodes et kits pour la synthese d'arn sens | |
DK0721988T3 (da) | Kimærisk oligonucleotid og dets anvendelse ved opnåelse af transkripter af en nucleinsyre | |
WO1997041260A3 (fr) | Amplification soustractive | |
SE8801070D0 (sv) | Method for immobilizing a dna sequence on a solid support | |
HK145894A (en) | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids and kits therefor | |
EP1350841A3 (fr) | ADN polymérase thermostable ou thermoactif ayant une activité 3' - 5' exonucléase atténuée | |
DE69837913D1 (de) | Verfahren zur vervielfältigung von nukleinsäure | |
DE69627698D1 (de) | Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren | |
ATE205543T1 (de) | Verfahren zur verbesserung der nukleinsäureamplifizierung | |
NO20006675L (no) | Fremgangsmåte for fremstilling av höyt varierte bibliotek | |
US5571669A (en) | Transcription and nucleic acid sequence determination with short primer DNA/RNA molecules and RNA polymerase | |
DE69837711D1 (de) | Leistungstarke verknüpfung von nukleinsäuresegmenten | |
ATE319861T1 (de) | Verbesserte methoden zur erzeugung einer vielzahl von rna-kopien | |
AR018099A1 (es) | Metodo de bloqueo de sintesis de la 17beta-hidroxiesteroide dehidrogenasa del tipo 5 y metodo para interferir su expresion | |
ATE307901T1 (de) | Amplifikation und sequenzierung von primer-paaren und deren verwendung | |
Bertling et al. | Determination of 5'ends of specific mRNAs by DNA ligase-dependent amplification. | |
ZA989950B (en) | Transcription based amplification of double stranded DNA targets | |
MXPA02003422A (es) | Alteracion de la expresion de genes con adnss producido in vivo. | |
WO2005010188A3 (fr) | Arn pouvant moduler la mise au silence de la chromatine | |
TW200508394A (en) | Method of detecting micrometastasis |