HK1219300A1 - 用於在基於乳液的微流體中測序的系統和方法 - Google Patents

用於在基於乳液的微流體中測序的系統和方法

Info

Publication number
HK1219300A1
HK1219300A1 HK16107351.0A HK16107351A HK1219300A1 HK 1219300 A1 HK1219300 A1 HK 1219300A1 HK 16107351 A HK16107351 A HK 16107351A HK 1219300 A1 HK1219300 A1 HK 1219300A1
Authority
HK
Hong Kong
Prior art keywords
sequencing
systems
methods
emulsion based
based microfluidics
Prior art date
Application number
HK16107351.0A
Other languages
English (en)
Inventor
‧希利
Original Assignee
Gnubio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gnubio Inc filed Critical Gnubio Inc
Publication of HK1219300A1 publication Critical patent/HK1219300A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/20—Sequence assembly

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
HK16107351.0A 2013-05-29 2016-06-23 用於在基於乳液的微流體中測序的系統和方法 HK1219300A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361828582P 2013-05-29 2013-05-29
PCT/US2014/040082 WO2014194131A2 (en) 2013-05-29 2014-05-29 Systems and methods for sequencing in emulsion based microfluidics

Publications (1)

Publication Number Publication Date
HK1219300A1 true HK1219300A1 (zh) 2017-03-31

Family

ID=51989533

Family Applications (1)

Application Number Title Priority Date Filing Date
HK16107351.0A HK1219300A1 (zh) 2013-05-29 2016-06-23 用於在基於乳液的微流體中測序的系統和方法

Country Status (5)

Country Link
US (2) US9809851B2 (zh)
EP (1) EP3004391B1 (zh)
CN (1) CN105431553B (zh)
HK (1) HK1219300A1 (zh)
WO (1) WO2014194131A2 (zh)

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EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
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WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
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EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
SG193436A1 (en) 2011-03-30 2013-10-30 Gnubio Inc Injection of multiple volumes into or out of droplets
US9816931B2 (en) 2011-03-31 2017-11-14 Bio-Rad Laboratories, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
WO2012135201A1 (en) 2011-03-31 2012-10-04 Gnubio, Inc. Scalable spectroscopic detection and measurement
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US9821312B2 (en) 2012-09-12 2017-11-21 Bio-Rad Laboratories, Inc. Integrated microfluidic system, method and kit for performing assays
CN105074303B (zh) 2013-01-25 2018-04-10 基纽拜奥股份有限公司 实施液滴膨胀的系统和方法
US20160076082A1 (en) * 2013-04-26 2016-03-17 Gnubio, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
EP3004813A4 (en) 2013-05-29 2016-12-21 Gnubio Inc OPTICAL SYSTEM OF DISCREET, QUICK AND CHEAP MEASUREMENT
EP3039119A4 (en) 2013-08-27 2017-04-05 GnuBIO, Inc. Microfluidic devices and methods of their use
CN105636697B (zh) 2013-09-30 2018-06-12 基纽拜奥股份有限公司 微流体盒装置和使用方法以及组件
EP3074122A4 (en) 2013-11-27 2017-11-29 Bio-Rad Laboratories, Inc. Microfluidic droplet packing
US9683792B2 (en) 2014-06-30 2017-06-20 Bio-Rad Laboratories, Inc. Floating thermal contact enabled PCR
CN107406886A (zh) * 2015-01-23 2017-11-28 哈佛学院院长及董事 用于在液滴内扩增或克隆的系统、方法和试剂盒
WO2016149096A1 (en) 2015-03-13 2016-09-22 President And Fellows Of Harvard College Determination of cells using amplification
US10809250B2 (en) 2015-08-25 2020-10-20 Bio-Rad Laboratories, Inc. Digital immunoassay
CN108291223B (zh) * 2015-11-20 2022-06-07 生物辐射实验室股份有限公司 液滴测序中的稀疏标识空间
EP3397764A4 (en) * 2015-12-30 2019-05-22 Bio-Rad Laboratories, Inc. DIGITAL QUANTIFICATION OF PROTEINS
CN105821482B (zh) * 2016-04-29 2018-04-10 李星军 一种生化微反应体系、高通量测序的建库仪及应用
US11118217B2 (en) 2017-01-30 2021-09-14 Bio-Rad Laboratories, Inc. Emulsion compositions and methods of their use
CN114829626A (zh) 2019-10-10 2022-07-29 1859公司 用于微流体筛选的方法和系统
WO2023192815A2 (en) * 2022-03-28 2023-10-05 Baylor College Of Medicine Taxonomic signatures and methods of determining the same

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WO2012135201A1 (en) 2011-03-31 2012-10-04 Gnubio, Inc. Scalable spectroscopic detection and measurement
US9816931B2 (en) 2011-03-31 2017-11-14 Bio-Rad Laboratories, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
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WO2013122826A1 (en) 2012-02-14 2013-08-22 Gnubio, Inc. Cascaded addition of target specific universal adapters to nucleic acids
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US9821312B2 (en) 2012-09-12 2017-11-21 Bio-Rad Laboratories, Inc. Integrated microfluidic system, method and kit for performing assays
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CN105074303B (zh) 2013-01-25 2018-04-10 基纽拜奥股份有限公司 实施液滴膨胀的系统和方法
US20160076082A1 (en) 2013-04-26 2016-03-17 Gnubio, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
EP3004813A4 (en) 2013-05-29 2016-12-21 Gnubio Inc OPTICAL SYSTEM OF DISCREET, QUICK AND CHEAP MEASUREMENT
EP3039119A4 (en) 2013-08-27 2017-04-05 GnuBIO, Inc. Microfluidic devices and methods of their use
CN105636697B (zh) 2013-09-30 2018-06-12 基纽拜奥股份有限公司 微流体盒装置和使用方法以及组件

Also Published As

Publication number Publication date
US11053541B2 (en) 2021-07-06
US20150024945A1 (en) 2015-01-22
WO2014194131A3 (en) 2015-02-05
WO2014194131A2 (en) 2014-12-04
US20180073074A1 (en) 2018-03-15
EP3004391A4 (en) 2017-05-31
CN105431553A (zh) 2016-03-23
CN105431553B (zh) 2020-02-07
EP3004391A2 (en) 2016-04-13
US9809851B2 (en) 2017-11-07
EP3004391B1 (en) 2019-03-27

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