GB954552A - Production of l (+)-glutamic acid - Google Patents
Production of l (+)-glutamic acidInfo
- Publication number
- GB954552A GB954552A GB2598960A GB2598960A GB954552A GB 954552 A GB954552 A GB 954552A GB 2598960 A GB2598960 A GB 2598960A GB 2598960 A GB2598960 A GB 2598960A GB 954552 A GB954552 A GB 954552A
- Authority
- GB
- United Kingdom
- Prior art keywords
- inhibitor
- biotin
- glutamic acid
- penicillin
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L(+)-glutamic acid is prepared by aqueous aerobic fermentation of a nutrient medium containing sources of carbon and nitrogen, with a biotin-requiring, glutamic acid producing microorganism, the medium containing biotin in excess of the amount required for optimum production of the glutamic acid, an inhibitor being added to the fermenting medium subsequent to inoculation of the medium with the micro-organism, the inhibitor and the amount of it being such as to decrease, as fermentation proceeds, the intracellular L(+)-glutamic acid to a level less than half of that of a fermentation carried out without inhibitor and to increase the ratio of extracellular to intracellular L(+) glutamic acid to a value greater than 50, the amount of biotin, however, not being greater than 1 gram per litre of liquid medium. The inhibitor may be an antibiotic e.g., a penicillin, including Penicillin G, a -phenoxyethyl penicillin and a -phenoxymethyl penicillin which penicillins may be used in the form of a procaine, dibenzyl ethylenediamine, calcium, sodium, potassium or other salt thereof, cephalosporin C, oxamycin, novobiocin, tetracycline, oxytetracycline, chlortetracycline, streptomycin, dihydrostreptomycin sulphate, bacitracin, chloramphenicol and neomycin sulphate; phenol, resorcinol, sodium propionate and cetyl trimethyl ammonium bromide. The inhibitor is preferably added when the micro-organism has grown to the extent that would occur in the presence of about 1 to 5.0 parts of biotin per thousand million of medium. The amount of penicillin inhibitor is suitably 0.05-10 units per mol. of broth. The micro-organism may be a suitable strain of Bacillus subtilis, Escherichia coli, Micrococcus glutamicus, Bacillus-megaterium-Bacillus cereus intermediate type, Brevibacterium divaricatum, Brevibacterium aminagenes, Arthrobacter globiforme, Bacillus megaterium, Brevibacterium alanicum and Brevibacterium lactofermentus. The nutrient medium may comprise for example 20-37.5 parts of biotin per thousand million parts of broth. The carbon source may be dextrose, dextrin, beet molasses, cane molasses, corn syrup, black-strap molasses or hi-test molasses, some of which also provide the excess of biotin. The nitrogen source may be urea, ammonia, an ammonium salt or corn-steep liquor. The nutrient medium usually also comprises phosphates, sulphates, and magnesium, manganese, potassium and, may be, iron salts. The fermentation is preferably effected at a pH of 6.0 to 8.5, suitably accomplished by addition of urea, ammonia or an alkali metal hydroxide. Examples are given wherein Micrococcus glutamicus is used as micro-organism with excess of biotin and various of the above mentioned inhibitors or, comparatively in the absence of an inhibitor, or with inhibitor and biotin not in excess. Specifications 788,335, 826,097, 839,597, 844,910 and 849,280 are referred to.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US83214759A | 1959-08-07 | 1959-08-07 | |
US38413A US3080297A (en) | 1960-07-11 | 1960-07-11 | Production of glutamic acid |
Publications (1)
Publication Number | Publication Date |
---|---|
GB954552A true GB954552A (en) | 1964-04-08 |
Family
ID=26715175
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB2598960A Expired GB954552A (en) | 1959-08-07 | 1960-07-26 | Production of l (+)-glutamic acid |
Country Status (4)
Country | Link |
---|---|
BE (1) | BE593807A (en) |
CH (1) | CH457489A (en) |
DK (1) | DK113842B (en) |
GB (1) | GB954552A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107815476A (en) * | 2017-12-26 | 2018-03-20 | 天津北洋百川生物技术有限公司 | A kind of method that γ polyglutamic acids are produced using bacillus licheniformis |
-
1960
- 1960-07-26 GB GB2598960A patent/GB954552A/en not_active Expired
- 1960-08-05 BE BE593807A patent/BE593807A/en unknown
- 1960-09-07 CH CH1011460A patent/CH457489A/en unknown
- 1960-09-17 DK DK367960A patent/DK113842B/en unknown
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107815476A (en) * | 2017-12-26 | 2018-03-20 | 天津北洋百川生物技术有限公司 | A kind of method that γ polyglutamic acids are produced using bacillus licheniformis |
Also Published As
Publication number | Publication date |
---|---|
BE593807A (en) | 1961-02-06 |
CH457489A (en) | 1968-06-15 |
DK113842B (en) | 1969-05-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US3220929A (en) | Microbiological production of amino acid by reductive amination | |
GB1417067A (en) | Biological process for producing derivatives of amino acids | |
US2400143A (en) | Processes of fermentation | |
US3096252A (en) | Process for producing l-glutamic acid | |
GB954552A (en) | Production of l (+)-glutamic acid | |
US3594279A (en) | Process for producing l-tryptophan | |
US3087863A (en) | Amino acid synthesis | |
US3446705A (en) | Method for the production of 6-amino-penicillanic acid | |
US3682777A (en) | Process for producing penicillin derivatives | |
US3511752A (en) | Method for producing l-glutamic acid | |
US3450599A (en) | Process for producing l-glutamic acid and alpha-ketoglutaric acid | |
US4164445A (en) | Ethanol as the major source of carbon and energy in penicillin production | |
US3767529A (en) | Method of producing l-isoleucine by fermentation | |
US3216906A (en) | Method for producing glutamine by fermentation process | |
US4728610A (en) | Method for producing L-glutamic acid | |
US3254002A (en) | Method for producing glutamic acid | |
GB1354361A (en) | Process for the production of l-serine | |
US2965546A (en) | Biological conversion of 5alpha (11alpha)-dehydrotetracyclines to broad-spectrum antibiotics | |
US3616210A (en) | Process for producing l-glutamic acid | |
GB1200587A (en) | Process for producing l-lysine | |
US4452889A (en) | Method of producing inosine and/or guanosine | |
GB1176125A (en) | Process for Producing L-Threonine. | |
US3902968A (en) | Fermentation process | |
US3174908A (en) | Preparation of l-glutamic acid by fermentation | |
US3533914A (en) | Method of producing l-glutamic acid by fermentation |