GB954552A - Production of l (+)-glutamic acid - Google Patents
Production of l (+)-glutamic acidInfo
- Publication number
- GB954552A GB954552A GB2598960A GB2598960A GB954552A GB 954552 A GB954552 A GB 954552A GB 2598960 A GB2598960 A GB 2598960A GB 2598960 A GB2598960 A GB 2598960A GB 954552 A GB954552 A GB 954552A
- Authority
- GB
- United Kingdom
- Prior art keywords
- inhibitor
- biotin
- glutamic acid
- penicillin
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 title abstract 6
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 239000003112 inhibitor Substances 0.000 abstract 9
- 230000002401 inhibitory effect Effects 0.000 abstract 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract 7
- 229960002685 biotin Drugs 0.000 abstract 7
- 235000020958 biotin Nutrition 0.000 abstract 7
- 239000011616 biotin Substances 0.000 abstract 7
- 244000005700 microbiome Species 0.000 abstract 5
- 235000013379 molasses Nutrition 0.000 abstract 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 4
- 241000186146 Brevibacterium Species 0.000 abstract 3
- 241000186226 Corynebacterium glutamicum Species 0.000 abstract 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 abstract 3
- 238000000855 fermentation Methods 0.000 abstract 3
- 230000004151 fermentation Effects 0.000 abstract 3
- 235000015097 nutrients Nutrition 0.000 abstract 3
- UCSJYZPVAKXKNQ-HZYVHMACSA-N 1-[(1S,2R,3R,4S,5R,6R)-3-carbamimidamido-6-{[(2R,3R,4R,5S)-3-{[(2S,3S,4S,5R,6S)-4,5-dihydroxy-6-(hydroxymethyl)-3-(methylamino)oxan-2-yl]oxy}-4-formyl-4-hydroxy-5-methyloxolan-2-yl]oxy}-2,4,5-trihydroxycyclohexyl]guanidine Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 abstract 2
- 229960002989 Glutamic Acid Drugs 0.000 abstract 2
- 229940049954 Penicillin Drugs 0.000 abstract 2
- GHMLBKRAJCXXBS-UHFFFAOYSA-N Resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 abstract 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 2
- 229960000626 benzylpenicillin Drugs 0.000 abstract 2
- 239000004202 carbamide Substances 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- 229910052799 carbon Inorganic materials 0.000 abstract 2
- 235000013922 glutamic acid Nutrition 0.000 abstract 2
- 239000004220 glutamic acid Substances 0.000 abstract 2
- 230000003834 intracellular Effects 0.000 abstract 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N potassium Chemical class [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract 2
- 229910052700 potassium Inorganic materials 0.000 abstract 2
- 239000011591 potassium Chemical class 0.000 abstract 2
- DHPRQBPJLMKORJ-XRNKAMNCSA-N (4S,4aS,5aS,6S,12aR)-7-chloro-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide Chemical compound C1=CC(Cl)=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]4(O)C(=O)C3=C(O)C2=C1O DHPRQBPJLMKORJ-XRNKAMNCSA-N 0.000 abstract 1
- GSPKOIJAMLDYCY-RMIBZTJPSA-N 2-[(1R,2R,3S,4R,5R,6S)-3-(diaminomethylideneamino)-4-[(2R,3R,4R,5S)-3-[(2S,3S,4S,5R,6S)-4,5-dihydroxy-6-(hydroxymethyl)-3-(methylamino)oxan-2-yl]oxy-4-hydroxy-4-(hydroxymethyl)-5-methyloxolan-2-yl]oxy-2,5,6-trihydroxycyclohexyl]guanidine;sulfuric acid Chemical compound OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](CO)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](N=C(N)N)[C@H](O)[C@@H](N=C(N)N)[C@H](O)[C@H]1O GSPKOIJAMLDYCY-RMIBZTJPSA-N 0.000 abstract 1
- 241000186063 Arthrobacter Species 0.000 abstract 1
- 241000209134 Arundinaria Species 0.000 abstract 1
- 241000194107 Bacillus megaterium Species 0.000 abstract 1
- 240000008371 Bacillus subtilis Species 0.000 abstract 1
- 229940075615 Bacillus subtilis Drugs 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 229960003071 Bacitracin Drugs 0.000 abstract 1
- 108010001478 Bacitracin Proteins 0.000 abstract 1
- 235000016068 Berberis vulgaris Nutrition 0.000 abstract 1
- 241000335053 Beta vulgaris Species 0.000 abstract 1
- YJQPYGGHQPGBLI-KGSXXDOSSA-N Cathomycin Chemical compound O1C(C)(C)[C@H](OC)[C@@H](OC(N)=O)[C@@H](O)[C@@H]1OC1=CC=C(C(O)=C(NC(=O)C=2C=C(CC=C(C)C)C(O)=CC=2)C(=O)O2)C2=C1C YJQPYGGHQPGBLI-KGSXXDOSSA-N 0.000 abstract 1
- 241001660259 Cereus <cactus> Species 0.000 abstract 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 abstract 1
- 229960005091 Chloramphenicol Drugs 0.000 abstract 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N Chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 abstract 1
- 229960004475 Chlortetracycline Drugs 0.000 abstract 1
- 239000004099 Chlortetracycline Substances 0.000 abstract 1
- DYDCUQKUCUHJBH-UWTATZPHSA-N D-cycloserine Chemical compound N[C@@H]1CONC1=O DYDCUQKUCUHJBH-UWTATZPHSA-N 0.000 abstract 1
- 229920001353 Dextrin Polymers 0.000 abstract 1
- 239000004375 Dextrin Substances 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- ACTNHJDHMQSOGL-UHFFFAOYSA-N N',N'-dibenzylethane-1,2-diamine Chemical class C=1C=CC=CC=1CN(CCN)CC1=CC=CC=C1 ACTNHJDHMQSOGL-UHFFFAOYSA-N 0.000 abstract 1
- 229960002950 Novobiocin Drugs 0.000 abstract 1
- 229960000625 Oxytetracycline Drugs 0.000 abstract 1
- 239000004100 Oxytetracycline Substances 0.000 abstract 1
- IWVCMVBTMGNXQD-PXOLEDIWSA-N Oxytetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3[C@H](O)[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-PXOLEDIWSA-N 0.000 abstract 1
- -1 Penicillin G Chemical compound 0.000 abstract 1
- 229940056360 Penicillin G Drugs 0.000 abstract 1
- MFDFERRIHVXMIY-UHFFFAOYSA-N Procaine Chemical class CCN(CC)CCOC(=O)C1=CC=C(N)C=C1 MFDFERRIHVXMIY-UHFFFAOYSA-N 0.000 abstract 1
- JXKPEJDQGNYQSM-UHFFFAOYSA-M Sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 abstract 1
- 229960003212 Sodium propionate Drugs 0.000 abstract 1
- 229960005322 Streptomycin Drugs 0.000 abstract 1
- 229960002180 Tetracycline Drugs 0.000 abstract 1
- OFVLGDICTFRJMM-WESIUVDSSA-N Tetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O OFVLGDICTFRJMM-WESIUVDSSA-N 0.000 abstract 1
- 239000004098 Tetracycline Substances 0.000 abstract 1
- 241000209149 Zea Species 0.000 abstract 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 1
- 238000010564 aerobic fermentation Methods 0.000 abstract 1
- 150000008044 alkali metal hydroxides Chemical class 0.000 abstract 1
- 150000003863 ammonium salts Chemical class 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- CLKOFPXJLQSYAH-ABRJDSQDSA-N bacitracin A Chemical compound C1SC([C@@H](N)[C@@H](C)CC)=N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]1C(=O)N[C@H](CCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2N=CNC=2)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCCCC1 CLKOFPXJLQSYAH-ABRJDSQDSA-N 0.000 abstract 1
- 230000003115 biocidal Effects 0.000 abstract 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N calcium Chemical class [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
- 239000011575 calcium Chemical class 0.000 abstract 1
- 229910052791 calcium Inorganic materials 0.000 abstract 1
- HOKIDJSKDBPKTQ-GLXFQSAKSA-M cephalosporin C(1-) Chemical compound S1CC(COC(=O)C)=C(C([O-])=O)N2C(=O)[C@@H](NC(=O)CCC[C@@H]([NH3+])C([O-])=O)[C@@H]12 HOKIDJSKDBPKTQ-GLXFQSAKSA-M 0.000 abstract 1
- 235000019365 chlortetracycline Nutrition 0.000 abstract 1
- 235000005822 corn Nutrition 0.000 abstract 1
- 235000005824 corn Nutrition 0.000 abstract 1
- 229960003077 cycloserine Drugs 0.000 abstract 1
- 235000019425 dextrin Nutrition 0.000 abstract 1
- 239000008121 dextrose Substances 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 159000000014 iron salts Chemical class 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 abstract 1
- 239000011777 magnesium Substances 0.000 abstract 1
- 229910052749 magnesium Inorganic materials 0.000 abstract 1
- PWHULOQIROXLJO-UHFFFAOYSA-N manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 abstract 1
- 229910052748 manganese Inorganic materials 0.000 abstract 1
- 239000011572 manganese Substances 0.000 abstract 1
- OIXVKQDWLFHVGR-WQDIDPJDSA-N neomycin B sulfate Chemical compound OS(O)(=O)=O.N[C@@H]1[C@@H](O)[C@H](O)[C@H](CN)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](N)C[C@@H](N)[C@@H]2O)O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CN)O2)N)O[C@@H]1CO OIXVKQDWLFHVGR-WQDIDPJDSA-N 0.000 abstract 1
- 235000019366 oxytetracycline Nutrition 0.000 abstract 1
- 150000002960 penicillins Chemical class 0.000 abstract 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract 1
- 235000021317 phosphate Nutrition 0.000 abstract 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 abstract 1
- 229960004919 procaine Drugs 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N sodium Chemical class [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000011734 sodium Chemical class 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 235000010334 sodium propionate Nutrition 0.000 abstract 1
- 239000004324 sodium propionate Substances 0.000 abstract 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 abstract 1
- 239000006188 syrup Substances 0.000 abstract 1
- 235000020357 syrup Nutrition 0.000 abstract 1
- 235000019364 tetracycline Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
Abstract
L(+)-glutamic acid is prepared by aqueous aerobic fermentation of a nutrient medium containing sources of carbon and nitrogen, with a biotin-requiring, glutamic acid producing microorganism, the medium containing biotin in excess of the amount required for optimum production of the glutamic acid, an inhibitor being added to the fermenting medium subsequent to inoculation of the medium with the micro-organism, the inhibitor and the amount of it being such as to decrease, as fermentation proceeds, the intracellular L(+)-glutamic acid to a level less than half of that of a fermentation carried out without inhibitor and to increase the ratio of extracellular to intracellular L(+) glutamic acid to a value greater than 50, the amount of biotin, however, not being greater than 1 gram per litre of liquid medium. The inhibitor may be an antibiotic e.g., a penicillin, including Penicillin G, a -phenoxyethyl penicillin and a -phenoxymethyl penicillin which penicillins may be used in the form of a procaine, dibenzyl ethylenediamine, calcium, sodium, potassium or other salt thereof, cephalosporin C, oxamycin, novobiocin, tetracycline, oxytetracycline, chlortetracycline, streptomycin, dihydrostreptomycin sulphate, bacitracin, chloramphenicol and neomycin sulphate; phenol, resorcinol, sodium propionate and cetyl trimethyl ammonium bromide. The inhibitor is preferably added when the micro-organism has grown to the extent that would occur in the presence of about 1 to 5.0 parts of biotin per thousand million of medium. The amount of penicillin inhibitor is suitably 0.05-10 units per mol. of broth. The micro-organism may be a suitable strain of Bacillus subtilis, Escherichia coli, Micrococcus glutamicus, Bacillus-megaterium-Bacillus cereus intermediate type, Brevibacterium divaricatum, Brevibacterium aminagenes, Arthrobacter globiforme, Bacillus megaterium, Brevibacterium alanicum and Brevibacterium lactofermentus. The nutrient medium may comprise for example 20-37.5 parts of biotin per thousand million parts of broth. The carbon source may be dextrose, dextrin, beet molasses, cane molasses, corn syrup, black-strap molasses or hi-test molasses, some of which also provide the excess of biotin. The nitrogen source may be urea, ammonia, an ammonium salt or corn-steep liquor. The nutrient medium usually also comprises phosphates, sulphates, and magnesium, manganese, potassium and, may be, iron salts. The fermentation is preferably effected at a pH of 6.0 to 8.5, suitably accomplished by addition of urea, ammonia or an alkali metal hydroxide. Examples are given wherein Micrococcus glutamicus is used as micro-organism with excess of biotin and various of the above mentioned inhibitors or, comparatively in the absence of an inhibitor, or with inhibitor and biotin not in excess. Specifications 788,335, 826,097, 839,597, 844,910 and 849,280 are referred to.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83214759A | 1959-08-07 | 1959-08-07 | |
| US38413A US3080297A (en) | 1960-07-11 | 1960-07-11 | Production of glutamic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB954552A true GB954552A (en) | 1964-04-08 |
Family
ID=26715175
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2598960A Expired GB954552A (en) | 1959-08-07 | 1960-07-26 | Production of l (+)-glutamic acid |
Country Status (4)
| Country | Link |
|---|---|
| BE (1) | BE593807A (en) |
| CH (1) | CH457489A (en) |
| DK (1) | DK113842B (en) |
| GB (1) | GB954552A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107815476A (en) * | 2017-12-26 | 2018-03-20 | 天津北洋百川生物技术有限公司 | A kind of method that γ polyglutamic acids are produced using bacillus licheniformis |
-
1960
- 1960-07-26 GB GB2598960A patent/GB954552A/en not_active Expired
- 1960-08-05 BE BE593807A patent/BE593807A/en unknown
- 1960-09-07 CH CH1011460A patent/CH457489A/en unknown
- 1960-09-17 DK DK367960A patent/DK113842B/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107815476A (en) * | 2017-12-26 | 2018-03-20 | 天津北洋百川生物技术有限公司 | A kind of method that γ polyglutamic acids are produced using bacillus licheniformis |
Also Published As
| Publication number | Publication date |
|---|---|
| DK113842B (en) | 1969-05-05 |
| CH457489A (en) | 1968-06-15 |
| BE593807A (en) | 1961-02-06 |
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