GB837993A - A process for the preparation of levans - Google Patents
A process for the preparation of levansInfo
- Publication number
- GB837993A GB837993A GB36553/57A GB3655357A GB837993A GB 837993 A GB837993 A GB 837993A GB 36553/57 A GB36553/57 A GB 36553/57A GB 3655357 A GB3655357 A GB 3655357A GB 837993 A GB837993 A GB 837993A
- Authority
- GB
- United Kingdom
- Prior art keywords
- levan
- levans
- molecular weight
- ions
- adsorbing agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Levans of predetermined molecular weight are prepared by the interaction of levan-forming micro-organisms or cell-free levan-forming enzymes formed by such micro-organisms, and cane sugar, in an aqueous medium, characterized in that said interaction is carried out in the presence of a levan sucrase-adsorbing agent and in that the medium contains ions for regulating the degree of adsorption of levan sucrase by the adsorbing agents. The preferred micro-organism is Bacillus-subtilis. The adsorbing agent may be calcium phosphate gel, bentonite, activated carbon, asbestos, cellulose powder, kieselguhr, silica gel, aluminium hydroxide, starch, soya powder, magnesium phosphate, lanthanum ribonucleate or the denatured proteins which separate on heating corn steep water. Suitable ions are alkali metal, chloride, sulphate, orthophosphate, acetate, lactate or propionate ions. The molecular weight of the levans produced depends on the degree of adsorption of levan sucrase which can be controlled by varying the amount of adsorbent materials and/or the concentration of ions used. Levans intended for use as blood substitutes may be purified by colloido-chemical processes and then dissolved in blood-isotonic solutions.ALSO:A fermentation process for the preparation of levans of predetermined molecular weight by the interaction of levan-forming micro-organisms and cane sugar in an aqueous medium is characterized in that a levan sucrose-adsorbing agent is present and the medium contains ions for regulating the degree of adsorption of levan sucrose by the adsorbing agent. The preferred micro-organism is Bacillus subtilis. The adsorbing agent may be calcium phosphate gel, bentonite, activated carbon, asbestos, cellulose powder, kieselguhr, silica gel, aluminium hydroxide, starch, soya powder, magnesium phosphate, lanthanum ribonucleate or the denatured proteins which separate on heating corn steep water. Suitable ions are alkali metal, chloride, sulphate, orthophosphate, acetate, lactate or propionate ions. Levans for use as blood substitutes, having a molecular weight of about 65,000, prepared by this process may be purified by known colloido-chemical processes and then dissolved in blood-isotonic solutions, e.g. a 6% solution of the levan in a tyrode solution or an 0.9% sodium chloride solution. Levans of low molecular weight of levans of high molecular weight also produced by the above process may be used as thickening agents and pharmaceutical carriers in tablets.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE837993X | 1956-11-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB837993A true GB837993A (en) | 1960-06-22 |
Family
ID=6761561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB36553/57A Expired GB837993A (en) | 1956-11-23 | 1957-11-22 | A process for the preparation of levans |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB837993A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003010325A1 (en) * | 2001-07-25 | 2003-02-06 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | Process of producing mannitol and homopolysaccharides |
-
1957
- 1957-11-22 GB GB36553/57A patent/GB837993A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003010325A1 (en) * | 2001-07-25 | 2003-02-06 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | Process of producing mannitol and homopolysaccharides |
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