GB791186A - Improvements in and relating to intrinsic factor preparations - Google Patents

Improvements in and relating to intrinsic factor preparations

Info

Publication number
GB791186A
GB791186A GB9564/56A GB956456A GB791186A GB 791186 A GB791186 A GB 791186A GB 9564/56 A GB9564/56 A GB 9564/56A GB 956456 A GB956456 A GB 956456A GB 791186 A GB791186 A GB 791186A
Authority
GB
United Kingdom
Prior art keywords
intrinsic factor
potassium
precipitate
per cent
adjusted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB9564/56A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Armour and Co
Original Assignee
Armour and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Armour and Co filed Critical Armour and Co
Publication of GB791186A publication Critical patent/GB791186A/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients

Abstract

An intrinsic factor preparation of enhanced potency is prepared by extracting hog pyloric mucosa with an aqueous acidic solution at pH 2.0 to 5.0 and fractionating the so-formed extract by adding ethanol (or an aliphatic alcohol or ketone having less than 5 carbon atoms) until the concentration is between 20 and 60 per cent (preferably 45 to 55 per cent), by volume to obtain a precipitate of enhanced intrinsic factor potency. The pyloric mucosa may be comminuted, if desired after being frozen to prevent loss of activity. The extraction may be carried out at a temperature below 15 DEG C., preferably below 5 DEG C., the pH being adjusted by means of hydrochloric, sulphuric, phosphoric, lactic or citric acid. In the fractionation step the pH may be adjusted to between 4 to 6 (preferably 4.5 to 5.5) by means of sodium hydroxide, potassium hydroxide, calcium hydroxide, sodium bicarbonate, potassium bicarbonate, sodium carbonate or potassium carbonate. In addition to ethanol, methanol, acetone and methyl ethyl ketone are specified as liquid organic precipitating agents. The ionic strength during the fractionation should be less than 0.15, and the temperature below 0 DEG C., e.g. -5 DEG C. The precipitate may be separated, e.g. by centrifugal means, dissolved in ice-cold water, clarified and lyophilized. The examples give detailed accounts of the manner of carrying out the process of the invention. Specification 745,473 is referred to.
GB9564/56A 1955-03-28 1956-03-27 Improvements in and relating to intrinsic factor preparations Expired GB791186A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US791186XA 1955-03-28 1955-03-28

Publications (1)

Publication Number Publication Date
GB791186A true GB791186A (en) 1958-02-26

Family

ID=22148545

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9564/56A Expired GB791186A (en) 1955-03-28 1956-03-27 Improvements in and relating to intrinsic factor preparations

Country Status (1)

Country Link
GB (1) GB791186A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1979000880A1 (en) * 1978-04-04 1979-11-01 R Allen Quantitative testing for vitamin b12

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1979000880A1 (en) * 1978-04-04 1979-11-01 R Allen Quantitative testing for vitamin b12

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