GB764718A - Improvements in or relating to freeze-drying processes - Google Patents
Improvements in or relating to freeze-drying processesInfo
- Publication number
- GB764718A GB764718A GB23228/54A GB2322854A GB764718A GB 764718 A GB764718 A GB 764718A GB 23228/54 A GB23228/54 A GB 23228/54A GB 2322854 A GB2322854 A GB 2322854A GB 764718 A GB764718 A GB 764718A
- Authority
- GB
- United Kingdom
- Prior art keywords
- dextran
- freeze
- dried
- per cent
- free
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000004108 freeze drying Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title 1
- 229920002307 Dextran Polymers 0.000 abstract 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 3
- 239000008103 glucose Substances 0.000 abstract 3
- 239000000463 material Substances 0.000 abstract 3
- 229920000056 polyoxyethylene ether Polymers 0.000 abstract 3
- 238000001035 drying Methods 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 239000000080 wetting agent Substances 0.000 abstract 2
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 102000016911 Deoxyribonucleases Human genes 0.000 abstract 1
- 108010053770 Deoxyribonucleases Proteins 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 229940079919 digestives enzyme preparation Drugs 0.000 abstract 1
- 239000000284 extract Substances 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 239000005556 hormone Substances 0.000 abstract 1
- 229940088597 hormone Drugs 0.000 abstract 1
- 210000000056 organ Anatomy 0.000 abstract 1
- 229940051841 polyoxyethylene ether Drugs 0.000 abstract 1
- 239000000843 powder Substances 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B5/00—Drying solid materials or objects by processes not involving the application of heat
- F26B5/04—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum
- F26B5/06—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum the process involving freezing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Abstract
The moisture content of enzyme preparations to be freeze-dried is controlled by addition of aqueous dextran solution prior to freeze-drying. The dextran should have a molecular weight within the range from 10,000 to 100,000, and should be salt-free. Preferably from 1 to 10 per cent w/v. of the material to be dried is used. A compatible wetting agent (preferably acid-free such as the non-ionic polyoxyethylene ethers) may also be added. In order to prevent over drying, glucose or other inert substance which strongly retains water may also be added to the dextran solution. In an Example (4), a crude culture filtrate containing deoxyribonuclease is mixed with sufficient dextran powder to give a 5 per cent w/v. of dextran in solution, and the so-formed solution is freeze-dried.ALSO:The moisture content of sensitive materials to be freeze-dried such as vaccines, hormones, organ extracts and viruses is controlled by addition of aqueous dextran solution prior to freeze-drying. The dextran should have a molecular weight within the range from 10,000 to 100,000, and should be salt-free. Preferably from 1 to 10 per cent w/v of the material to be dried is used. A compatible wetting agent (preferably acid-free such as the non-ionic polyoxyethylene ethers) may also be added. In order to prevent over drying, glucose or other inert substance which strongly retains water may also be added to the dextran solution. In an example viable B.C.G. organisms (Bacillus of Calmette and Guerin) are collected from a culture medium and suspended in 6 per cent w/v dextran in 5 per cent glucose containing 1/4000 parts of a polyoxyethylene ether. The suspension is filled into ampoules and freeze dried.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB23228/54A GB764718A (en) | 1954-08-10 | 1954-08-10 | Improvements in or relating to freeze-drying processes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB23228/54A GB764718A (en) | 1954-08-10 | 1954-08-10 | Improvements in or relating to freeze-drying processes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB764718A true GB764718A (en) | 1957-01-02 |
Family
ID=10192239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB23228/54A Expired GB764718A (en) | 1954-08-10 | 1954-08-10 | Improvements in or relating to freeze-drying processes |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB764718A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2035805A1 (en) * | 1968-08-20 | 1970-12-24 | Miles Lab | |
| EP0081913A1 (en) * | 1981-12-11 | 1983-06-22 | JOHN WYETH & BROTHER LIMITED | Process and apparatus for freezing a liquid medium |
| WO2003049752A2 (en) * | 2001-12-11 | 2003-06-19 | Institut Pasteur | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| WO2007135421A1 (en) * | 2006-05-24 | 2007-11-29 | Cab International | Method for preservation of micro-organisms |
-
1954
- 1954-08-10 GB GB23228/54A patent/GB764718A/en not_active Expired
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2035805A1 (en) * | 1968-08-20 | 1970-12-24 | Miles Lab | |
| EP0081913A1 (en) * | 1981-12-11 | 1983-06-22 | JOHN WYETH & BROTHER LIMITED | Process and apparatus for freezing a liquid medium |
| US4470202A (en) * | 1981-12-11 | 1984-09-11 | John Weyeth & Brother Limited | Process and apparatus for freezing a liquid medium |
| AU2002356387B2 (en) * | 2001-12-11 | 2007-09-13 | Institut National De La Sante Et De La Recherche Medicale | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| WO2003049752A3 (en) * | 2001-12-11 | 2003-11-20 | Pasteur Institut | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| JP2005516915A (en) * | 2001-12-11 | 2005-06-09 | インスティティ・パスツール | Gram-positive bacterial preparations for the treatment of diseases involving immune dysregulation |
| WO2003049752A2 (en) * | 2001-12-11 | 2003-06-19 | Institut Pasteur | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| AU2002356387C1 (en) * | 2001-12-11 | 2008-03-06 | Institut National De La Sante Et De La Recherche Medicale | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| US7871627B2 (en) | 2001-12-11 | 2011-01-18 | Institut Pasteur | Gram positive bacteria preparations for the treatment of disease comprising an immune dysregulation |
| KR101048798B1 (en) * | 2001-12-11 | 2011-07-15 | 앵스티띠 나셔날 드 라 쌍뜨 에 드 라 르셰르슈 메디깔 | Gram-positive bacteria preparations for the treatment of diseases including immunomodulatory disorder |
| KR101124363B1 (en) * | 2001-12-11 | 2012-03-21 | 앵스티띠 나셔날 드 라 쌍뜨 에 드 라 르셰르슈 메디깔 | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| CN1602199B (en) * | 2001-12-11 | 2012-04-04 | 巴斯德研究院 | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| EP2316466A3 (en) * | 2001-12-11 | 2012-04-11 | Institut Pasteur | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| US8404250B2 (en) | 2001-12-11 | 2013-03-26 | Institut Pasteur | Gram positive bacteria preparations for the treatment of diseases comprising an immune dysregulation |
| WO2007135421A1 (en) * | 2006-05-24 | 2007-11-29 | Cab International | Method for preservation of micro-organisms |
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