GB2468624A - Cell culture system for pancreatic islands - Google Patents

Cell culture system for pancreatic islands Download PDF

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Publication number
GB2468624A
GB2468624A GB1012470A GB201012470A GB2468624A GB 2468624 A GB2468624 A GB 2468624A GB 1012470 A GB1012470 A GB 1012470A GB 201012470 A GB201012470 A GB 201012470A GB 2468624 A GB2468624 A GB 2468624A
Authority
GB
United Kingdom
Prior art keywords
cell culture
pancreatic islands
stem cells
islands
pancreatic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB1012470A
Other versions
GB201012470D0 (en
Inventor
Wendy Margaret Macfarlane
Moira Harrison
Clare Elizabeth Marriott
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Brighton
Original Assignee
University of Brighton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to GBGB0800524.1A priority Critical patent/GB0800524D0/en
Application filed by University of Brighton filed Critical University of Brighton
Priority to PCT/GB2009/050024 priority patent/WO2009090424A1/en
Publication of GB201012470D0 publication Critical patent/GB201012470D0/en
Publication of GB2468624A publication Critical patent/GB2468624A/en
Granted legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0676Pancreatic cells
    • C12N5/0677Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/08Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/34Sugars
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/11Epidermal growth factor [EGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/117Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/38Hormones with nuclear receptors
    • C12N2501/39Steroid hormones
    • C12N2501/392Sexual steroids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Abstract

Three-dimensional (3D) insulin-producing cell clusters derived from stem cells (preferably human embryonic stem cells) are provided by this invention, together with a method for their production using a microgravity bioreactor cell culture system.
GB1012470A 2008-01-14 2009-01-14 Cell culture system for pancreatic islands Granted GB2468624A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GBGB0800524.1A GB0800524D0 (en) 2008-01-14 2008-01-14 Cell culture system
PCT/GB2009/050024 WO2009090424A1 (en) 2008-01-14 2009-01-14 Cell culture system for pancreatic islands

Publications (2)

Publication Number Publication Date
GB201012470D0 GB201012470D0 (en) 2010-09-08
GB2468624A true GB2468624A (en) 2010-09-15

Family

ID=39144815

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB0800524.1A Ceased GB0800524D0 (en) 2008-01-14 2008-01-14 Cell culture system
GB1012470A Granted GB2468624A (en) 2008-01-14 2009-01-14 Cell culture system for pancreatic islands

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB0800524.1A Ceased GB0800524D0 (en) 2008-01-14 2008-01-14 Cell culture system

Country Status (7)

Country Link
US (1) US20110027880A1 (en)
EP (1) EP2238240A1 (en)
JP (1) JP2011509668A (en)
AU (1) AU2009204710A1 (en)
CA (1) CA2712112A1 (en)
GB (2) GB0800524D0 (en)
WO (1) WO2009090424A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2484873B (en) 2009-07-20 2014-04-30 Janssen Biotech Inc Differentiation of pancreatic endoderm cells.
ITTO20110432A1 (en) 2011-05-16 2011-08-15 Torino Politecnico MICROGRAVITY GENERATOR DEVICE.
WO2013130075A1 (en) * 2012-02-29 2013-09-06 The Regents Of The University Of California Culture system for stem cell propagation and neural and oligodendrocyte specification in microgravity
BR112015015714A2 (en) * 2012-12-31 2017-07-11 Janssen Biotech Inc suspension and agglomeration of human pluripotent cells for differentiation into pancreatic endocrine cells
BR112015015701A2 (en) 2012-12-31 2017-07-11 Janssen Biotech Inc differentiation of human embryonic stem cells into endocrine pancreatic cells using hb9 regulators
WO2015065524A2 (en) * 2013-11-01 2015-05-07 Janssen Biotech, Inc. Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
CN111635883A (en) 2013-12-16 2020-09-08 弗雷森纽斯医疗护理德国有限责任公司 Islet-like cell structure and preparation method thereof
IT201800010212A1 (en) 2018-11-09 2020-05-09 Cellex S R L Suspension cell culture device
KR102275454B1 (en) * 2020-05-07 2021-07-09 건국대학교 산학협력단 A Method for Differentiation of Mesenchymal Stem Cells from Pluripotent Stem Cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997016536A1 (en) * 1995-10-30 1997-05-09 Vivorx, Inc. Method for ex vivo proliferation and differentiation of adult pancreatic islet cells, media useful therefor and uses thereof
WO2004050827A2 (en) * 2002-12-05 2004-06-17 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002102997A2 (en) * 2000-11-30 2002-12-27 Stemron Inc. Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same
US7947499B2 (en) * 2002-11-29 2011-05-24 Technion Research & Development Foundation Ltd. Method of dynamically culturing embryonic stem cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997016536A1 (en) * 1995-10-30 1997-05-09 Vivorx, Inc. Method for ex vivo proliferation and differentiation of adult pancreatic islet cells, media useful therefor and uses thereof
WO2004050827A2 (en) * 2002-12-05 2004-06-17 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Ann. N.Y. Acad. Sci., 2001, Cameron, D. et al., "Formation of sertoli cell-enriched...", pp.420-428 *
In Vitro Cell. Dev. Biol., Vol.35, 1999, Rose, M. et al., "Effects of microgravity...", pp.560-563 *
In Vitro Cell. Dev. Biol., Vol.37, 2001, Cameron, D. et al., "Formation of insulin-secreting...", pp.490-498 *
Nature Biotech., Vol.24, 2006, D'Amour, K. A. et al., "Production of pancreatic hormone-expressing...", pp.1392-1401 *
Science, Vol.292, 2001, Lumelsky, N. et al., "Differentiation of embryonic stem cells...", pp.1389-1394 *
Stem Cells, Vol.25, 2007, Jiang, J.et al., "Generation of insulin-producing...", pp.1940-1953 *

Also Published As

Publication number Publication date
WO2009090424A1 (en) 2009-07-23
JP2011509668A (en) 2011-03-31
GB0800524D0 (en) 2008-02-20
GB201012470D0 (en) 2010-09-08
AU2009204710A1 (en) 2009-07-23
US20110027880A1 (en) 2011-02-03
CA2712112A1 (en) 2009-07-23
EP2238240A1 (en) 2010-10-13

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