GB2406571A - Polypeptides with a signal sequence comprising an fivsi motif and olynucleotides encoding therefor - Google Patents

Polypeptides with a signal sequence comprising an fivsi motif and olynucleotides encoding therefor Download PDF

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Publication number
GB2406571A
GB2406571A GB0501988A GB0501988A GB2406571A GB 2406571 A GB2406571 A GB 2406571A GB 0501988 A GB0501988 A GB 0501988A GB 0501988 A GB0501988 A GB 0501988A GB 2406571 A GB2406571 A GB 2406571A
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GB
United Kingdom
Prior art keywords
motif
sequence
leader sequence
fivsi
olynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0501988A
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GB0501988D0 (en
GB2406571B (en
Inventor
Darrell Sleep
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Albumedix Ltd
Original Assignee
Delta Biotechnology Ltd
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Filing date
Publication date
Application filed by Delta Biotechnology Ltd filed Critical Delta Biotechnology Ltd
Publication of GB0501988D0 publication Critical patent/GB0501988D0/en
Publication of GB2406571A publication Critical patent/GB2406571A/en
Application granted granted Critical
Publication of GB2406571B publication Critical patent/GB2406571B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Abstract

The present invention provides a method for improving a leader sequence by insertion of a pentapeptide motif comprising -X 1 -X 2 -X 3 -X 4 -X 5 - and a polypeptide comprising (i) a leader sequence, the leader sequence comprising a (a) secretion pre sequence, and (b) the following motif: -X 1 -X 2 -X 3 -X 4 -X 5 - where X 1 is phenylalanine, tryptophan, or tyrosine, X 2 is isoleucine, leucine, valine, alanine or methionine, X 3 is leucine, valine, alanine or methionine, X 4 is serine or threonine and X 5 is isoleucine, valine, alanine or methionine; and (ii) a desired protein heterologous to the leader sequence. A polypeptide of the invention may additionally comprise, as part of the leader sequence, a secretion pro sequence. The invention also provides a polynucleotide comprising a sequence that encodes a polypeptide of the invention and a cell, preferably a yeast cell, comprising said polynucleotide.

Description

GB 2406571 A continuation
(72) Inventor(s): (58) Field of Search by ISA:
Darrell Sleep INT CL7 C12N Other: EPO-lnternal. CHEW ABS DatA, SEQUENCE (74) Agent and/or Address for Service: SEARCH, PAJ, WPI Data, BIOSIS, EMBASE, Eric Potter Clarkson EMBL Park View House, 58 The Ropewalk, NOTTINGHAM, NG1 5DD, United Kingdom
GB0501988A 2002-07-23 2003-07-23 Gene and polypeptide sequences Expired - Lifetime GB2406571B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0217033A GB0217033D0 (en) 2002-07-23 2002-07-23 Gene and polypeptide sequences
PCT/GB2003/003273 WO2004009819A2 (en) 2002-07-23 2003-07-23 Polypeptides with a signal sequence comprising an fivsi motif and olynucleotides encoding therefor

Publications (3)

Publication Number Publication Date
GB0501988D0 GB0501988D0 (en) 2005-03-09
GB2406571A true GB2406571A (en) 2005-04-06
GB2406571B GB2406571B (en) 2007-02-21

Family

ID=9940938

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0217033A Ceased GB0217033D0 (en) 2002-07-23 2002-07-23 Gene and polypeptide sequences
GB0501988A Expired - Lifetime GB2406571B (en) 2002-07-23 2003-07-23 Gene and polypeptide sequences

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB0217033A Ceased GB0217033D0 (en) 2002-07-23 2002-07-23 Gene and polypeptide sequences

Country Status (14)

Country Link
US (2) US8258102B2 (en)
EP (2) EP1530639B1 (en)
JP (1) JP4597668B2 (en)
KR (1) KR101246040B1 (en)
CN (1) CN1681930B (en)
AT (1) ATE527364T1 (en)
AU (1) AU2003254495B2 (en)
CA (1) CA2493432A1 (en)
DK (1) DK2390333T3 (en)
GB (2) GB0217033D0 (en)
HK (1) HK1071909A1 (en)
NZ (1) NZ537779A (en)
WO (1) WO2004009819A2 (en)
ZA (2) ZA200500622B (en)

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WO2006113681A2 (en) 2005-04-15 2006-10-26 Human Matrix Sciences, Llc Plant-derived elastin binding protein ligands and methods of using the same
WO2007039999A1 (en) * 2005-09-30 2007-04-12 Densei-Lambda Kabushiki Kaisha Battery pack
JP2010183885A (en) * 2009-02-13 2010-08-26 Kobe Univ Method for producing protein and expression vector used therefor
WO2017112847A1 (en) 2015-12-22 2017-06-29 Albumedix A/S Improved protein expression strains
EP3443001A4 (en) 2016-04-11 2020-04-29 Obsidian Therapeutics, Inc. Regulated biocircuit systems
ES2906997T3 (en) 2017-06-20 2022-04-21 Albumedix Ltd Enhanced protein expression strains
EP3806888B1 (en) 2018-06-12 2024-01-31 Obsidian Therapeutics, Inc. Pde5 derived regulatory constructs and methods of use in immunotherapy
EP3870600A1 (en) 2018-10-24 2021-09-01 Obsidian Therapeutics, Inc. Er tunable protein regulation
WO2020185632A1 (en) 2019-03-08 2020-09-17 Obsidian Therapeutics, Inc. Human carbonic anhydrase 2 compositions and methods for tunable regulation
CN114450308A (en) 2019-06-12 2022-05-06 黑曜石疗法公司 CA2 compositions and methods for regulatory modulation
EP3983537A1 (en) 2019-06-12 2022-04-20 Obsidian Therapeutics, Inc. Ca2 compositions and methods for tunable regulation
US20220348937A1 (en) 2019-09-06 2022-11-03 Obsidian Therapeutics, Inc. Compositions and methods for dhfr tunable protein regulation
US11739166B2 (en) 2020-07-02 2023-08-29 Davol Inc. Reactive polysaccharide-based hemostatic agent

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Also Published As

Publication number Publication date
WO2004009819A2 (en) 2004-01-29
CN1681930B (en) 2012-09-26
US20130059785A1 (en) 2013-03-07
ATE527364T1 (en) 2011-10-15
GB0217033D0 (en) 2002-08-28
JP4597668B2 (en) 2010-12-15
ZA200500622B (en) 2007-07-25
GB0501988D0 (en) 2005-03-09
US8258102B2 (en) 2012-09-04
CA2493432A1 (en) 2004-01-29
EP1530639B1 (en) 2011-10-05
NZ537779A (en) 2008-06-30
CN1681930A (en) 2005-10-12
GB2406571B (en) 2007-02-21
EP2390333A1 (en) 2011-11-30
EP2390333B1 (en) 2015-10-21
KR101246040B1 (en) 2013-03-27
WO2004009819A3 (en) 2004-03-25
EP1530639A2 (en) 2005-05-18
JP2006501818A (en) 2006-01-19
KR20050059046A (en) 2005-06-17
US9133265B2 (en) 2015-09-15
AU2003254495B2 (en) 2009-03-26
AU2003254495A1 (en) 2004-02-09
ZA200703235B (en) 2009-08-26
US20060183187A1 (en) 2006-08-17
HK1071909A1 (en) 2005-08-05
DK2390333T3 (en) 2015-12-14

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