GB2202549A - Foldable fermenter - Google Patents

Foldable fermenter Download PDF

Info

Publication number
GB2202549A
GB2202549A GB08706739A GB8706739A GB2202549A GB 2202549 A GB2202549 A GB 2202549A GB 08706739 A GB08706739 A GB 08706739A GB 8706739 A GB8706739 A GB 8706739A GB 2202549 A GB2202549 A GB 2202549A
Authority
GB
United Kingdom
Prior art keywords
fermenter
sterilisation
flexible material
constructed
storage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
GB08706739A
Other versions
GB8706739D0 (en
Inventor
Philip John Whitney
Original Assignee
Philip John Whitney
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip John Whitney filed Critical Philip John Whitney
Priority to GB08706739A priority Critical patent/GB2202549A/en
Publication of GB8706739D0 publication Critical patent/GB8706739D0/en
Publication of GB2202549A publication Critical patent/GB2202549A/en
Application status is Pending legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/26Constructional details, e.g. recesses, hinges flexible

Abstract

A fermenter for plant, animal and microbial cell culture is constructed of a flexible material such that it may be folded for sterilisation or storage. The fermenter may include rigid components or a rigid external support neither of which will not interfere with the folding of the fermenter for sterilisation or storage. <IMAGE>

Description

FOLDABLE FERMENTER This invention relates to the design and construction of fermenters for plants animal and microbial cell culture.

There are many designs of fermented but they all have in common the fact that they are rigid, i.e. cannot be folded into a small volume for sterilization and storage. This invention makes use of modern materials (e.g. plastics 5 plasitic composites and man-made fibres ) for the construction of fordable fermenters. Small fermenters can be cheaply manufactured and being fordable need little storage space so that they can be kept in significant numbers ready tor use when needed. Users would be expected to include schools, sixth form and other colleges Df further education, universities, research institutes and commercial research laboratories.Larger foldable fermenters have the advantage over their rigid counterparts that they do not need to be constucted as a pressure vessel and sterilized with live steam. Even large foldable fermenters can be folded into a volume small enough to be sterilized in an autoclave of normal dimensions. This elimates the need for the fermenter to be massively constructed of stainless steel to withstand the pressure of steam sterilization, which is the usual method of construction and sterilization of large fermenters.

Small fordable fermenters can be hung from a simple stand but larger foldable fermenters may need additional support either in the form of multiple support points or an external cage. As this cage would not come into contact with the organism being cultured or the culture medium it need not be sterile or corrosion resistant.

In this invention the body of the fermenter is constructed of materials rendering it foldable. There may be rigid components such as ports or stirrers but these will not be such as to interfere with the folding of the fermented refereed to later as semi-rigid ). The fermenter will be constructed of materials capable of withstanding sterilisation by one or more of ; heat (wet or dry), radiation or chemicals.

Specific embodyments of this of this invention will now be described by way Df example with reference to accompanying drawings and photographs. The prototypes in the photographs are constructed of natural high density polypropylene 30 microns in thickness5 but other materials and thicknesses may well prove suitable for both large and small scale foldable fermenters.

Figure 1. shows in perspective a small fordable fermenter sparged with a gas.

Figure 2. shows in section a small air-lift fermenter.

Figure 3. shows one design of a larger fermenter with support for the flexible membrane and a mechanical agitator Figure 4. shows a fully flexible and a semi-rigid fermenter folded for sterilisation or storage.

Photographs 1-3 show small fermenters empty and folded.

Photographs 4-5 show a small fermenter with a side port on a stand empty and full of culture medium.

Photogaph 6 shows a small fermenter without side port half filled with medium.

Photograph 7 shows a small flat bottomed isometric (cubic) fermenter sparged with air.

Photographs 8 k 9 show a small conical fermenter without and with aeration.

Photograph 10 shows various shapes of small fermenters empty and one folded for storage or sterilisation.

Photograph 11 shows the ability of the fermenter to cope with foaming of medium.

Claims (3)

1. A fermented constructed of a flexible material such that the fermenter may be folded for sterilisation and storage. The flexible material being capable of withstanding sterilisation by one or more of 5 heat (wet or dry) chemicals , ionising radiation.
2. A fermenter constructed largely of a flexible material as claimed in claim l. but including rigid components such as ports which will not interfere with the folding of the fermenter for sterilisation and storage.
3. A fermenter constructed of a flexible material as claimed in claims 1 K 2 but with a rigid external support which need not contact the organisms cultured and will therefore not need tD be sterilisable or corrosion resistant.
3. A fermenter constructed of a flexible material as claimed in claims 1 & 2 but with a rigid external support which need not contact the organisms cultured and will therefore not need to be sterilisable or corrosion resistant.
Amendments to the claims have been filed as follows CLAIMS I. A fermenter constructed of a flexible material such that the fermenter may be folded for sterilisation and storage. The fermenter to be fitted with bacteria-proof filters such that gases and, or liquids can enter or leave without microbially contaminating the fermenter. The whole structure being capable of withstanding sterilisation by one or more of , heat (wet or dry), chemicals , ionising radiation.
2. A fermenter constructed largely of a flexible material as claimed in claim 1. but including rigid components such as ports which will not interfere with the folding of the fermenter for sterilisation and storage.
GB08706739A 1987-03-20 1987-03-20 Foldable fermenter Pending GB2202549A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB08706739A GB2202549A (en) 1987-03-20 1987-03-20 Foldable fermenter

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB08706739A GB2202549A (en) 1987-03-20 1987-03-20 Foldable fermenter

Publications (2)

Publication Number Publication Date
GB8706739D0 GB8706739D0 (en) 1987-04-23
GB2202549A true GB2202549A (en) 1988-09-28

Family

ID=10614369

Family Applications (1)

Application Number Title Priority Date Filing Date
GB08706739A Pending GB2202549A (en) 1987-03-20 1987-03-20 Foldable fermenter

Country Status (1)

Country Link
GB (1) GB2202549A (en)

Cited By (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002167A1 (en) * 1988-08-23 1990-03-08 Anidral S.R.L. Kit for the reactivation of dehydrated microbial cultures, particularly of bacteria
EP0497330A1 (en) * 1991-01-31 1992-08-05 Boehringer Ingelheim Animal Health Inc. Process and apparatus for the surface culture of nucleated cells and cell culture-dependent substances
EP0576870A2 (en) * 1992-06-12 1994-01-05 Ben-Gurion University Of The Negev Research And Development Authority Microorganism growth apparatus
US5443985A (en) * 1993-07-22 1995-08-22 Alberta Research Council Cell culture bioreactor
US5476763A (en) * 1990-09-28 1995-12-19 Electrolux S.A.R.L. Medical transport assembly
WO1998013469A1 (en) * 1996-09-26 1998-04-02 Metabogal Ltd. Cell/tissue culturing device and method
FR2799138A1 (en) * 1999-10-05 2001-04-06 Opifex Disposable reactor for growing micro-organisms, has nested covers defining sealed compartments, pressurized gas stirs and circulates liquid in, and between, compartments
US6245555B1 (en) * 1998-09-01 2001-06-12 The Penn State Research Foundation Method and apparatus for aseptic growth or processing of biomass
DE10016554A1 (en) * 2000-04-03 2001-10-18 Rootec Ges Fuer Bioaktive Wirk Plant or animal tissue cultivation unit, comprises fermenter container, supply for liquid nutrients and gases, discharger for used nutrients, and carrier plates
US6432698B1 (en) * 1999-01-06 2002-08-13 Rutgers, The State University Disposable bioreactor for culturing microorganisms and cells
WO2003006633A1 (en) * 2001-07-09 2003-01-23 Alcan Packaging Pharmaceutical And Personal Care Inc. Disposable vessel
DE10164458A1 (en) * 2001-12-21 2003-07-10 Fraunhofer Ges Forschung Laboratory fermentation vessel, useful for production of phototrophic micro-organisms, comprises thermoplastic foil layers
WO2005067498A2 (en) * 2004-01-12 2005-07-28 Single Use Brx, Llc Bioreactor systems and disposable bioreactor
WO2005108546A2 (en) * 2004-04-27 2005-11-17 Baxter International Inc. Stirred-tank reactor system
EP1602715A2 (en) * 2004-06-02 2005-12-07 Millipore Corporation Disposable bioreactor
WO2005118771A2 (en) * 2004-06-04 2005-12-15 Xcellerex, Inc. Disposable bioreactor systems and methods
DE102004030378A1 (en) * 2004-06-23 2006-02-02 Kroon, Saide Z + L film reactor
WO2007050971A1 (en) * 2005-10-26 2007-05-03 Levtech, Inc. Bioreactor with mixer and sparger
DE102006021984A1 (en) * 2006-05-10 2007-11-15 Sartorius Biotech Gmbh One-way bioreactor for cultivating cells, includes flexible walls, side wall, bottom part and cover part limiting the reactor interior, in which rotation mixer comprising mixing element and mixer shank arranged at the cover part is arranged
WO2008014974A1 (en) * 2006-08-01 2008-02-07 Celljet Biotech Limited Liquid circulation system comprising an inflatable guide
US7357567B2 (en) 2001-04-10 2008-04-15 Levtech, Inc. Sterile fluid pumping or mixing system and related method
WO2008135991A2 (en) * 2007-05-07 2008-11-13 Protalix Ltd. Large scale disposable bioreactor
US7682067B2 (en) 2005-04-22 2010-03-23 Hyclone Laboratories, Inc. Mixing systems and related mixers
US7762716B2 (en) 2000-10-09 2010-07-27 Levtech, Inc. Mixing vessel with a fluid-agitating element supported by a roller bearing
US20100290308A1 (en) * 2000-10-09 2010-11-18 Terentiev Alexandre N Systems using a levitating, rotating pumping or mixing element and related methods
US7942925B2 (en) 2001-07-09 2011-05-17 Surpass Medical Ltd. Implantable intraluminal device and method of using same in treating aneurysms
US7951557B2 (en) 2003-04-27 2011-05-31 Protalix Ltd. Human lysosomal proteins from plant cell culture
US20110159590A1 (en) * 1996-09-26 2011-06-30 Protalix Ltd. Cell/tissue culturing device, system and method
US7992846B2 (en) 2004-01-07 2011-08-09 Atmi Packaging, Inc. Mixing bag with integral sparger and sensor receiver
EP2020433A3 (en) * 2007-07-30 2011-10-05 Xcellerex, Inc. Continuous perfusion bioreactor system
US8182137B2 (en) * 2000-10-09 2012-05-22 Atmi Packaging, Inc. Mixing bag or vessel with a fluid-agitating element
US8282267B2 (en) 2006-10-03 2012-10-09 Artelis S.A. Mixing system including a flexible bag, specific flexible bag and locating system for the mixing system
US8282269B2 (en) 2000-10-09 2012-10-09 Atmi Packaging, Inc. Mixing bag or vessel having a fluid-agitating element
US8603805B2 (en) 2005-04-22 2013-12-10 Hyclone Laboratories, Inc. Gas spargers and related container systems
US8608369B2 (en) 2011-01-07 2013-12-17 Hyclone Laboratories, Inc. Methods and systems for heating and mixing fluids
WO2014191947A1 (en) * 2013-05-30 2014-12-04 Rootec Bioactives Ag Bioreactor for obtaining secondary plant constituents
WO2015077663A1 (en) * 2013-11-21 2015-05-28 Distek Inc. Disposable bioreactors and methods for construction and use thereof
GB2522170A (en) * 2013-09-09 2015-07-22 Univ Swansea Bioreactor lid
US9314751B2 (en) 2011-01-07 2016-04-19 Life Technologies Corporation Methods and apparatus for mixing and shipping fluids
US9339026B2 (en) 2012-06-14 2016-05-17 Therapeutic Proteins International, LLC Pneumatically agitated and aerated single-use bioreactor
US9376655B2 (en) 2011-09-29 2016-06-28 Life Technologies Corporation Filter systems for separating microcarriers from cell culture solutions
EP2925427A4 (en) * 2012-11-29 2016-08-17 Emd Millipore Corp 2d low level mixing bag for storage and shipping
WO2016161159A1 (en) * 2015-03-31 2016-10-06 Heliae Development, Llc System for vitally supporting organisms and methods of providing and using the same
EP3004319A4 (en) * 2013-06-05 2017-01-25 GE Healthcare Bio-Sciences AB Disposable container and mixing system comprising the container
US9643133B2 (en) 2011-09-30 2017-05-09 Life Technologies Corporation Container with film sparger
US9687799B2 (en) 2014-03-17 2017-06-27 Advanced Scientifics, Inc. Transportable mixing system for biological and pharmaceutical materials
US9827541B1 (en) 2012-11-29 2017-11-28 Emd Millipore Corporation 2D low level mixing bag for storage and shipping
US10047337B2 (en) 2015-03-31 2018-08-14 Heliae Development Llc Method of mixotrophic culturing of microalgae in a flexible bioreactor
US10059918B2 (en) 2015-03-31 2018-08-28 Heliae Development Llc Method of vitally supporting microalgae in a flexible bioreactor
US10125346B2 (en) 2015-03-31 2018-11-13 Heliae Development Llc Bioreactor sterilization method for multiple uses
US10123940B2 (en) 2014-06-26 2018-11-13 Advanced Scientific, Inc. Bag assembly and system for use with a fluid
US10184099B2 (en) 2015-03-31 2019-01-22 Heliae Development Llc Flexible bioreactor and support structure system
US10184105B2 (en) 2015-03-31 2019-01-22 Heliae Development Llc Flexible bioreactor and support structure method
US10525425B2 (en) 2015-10-08 2020-01-07 Life Technologies Corporation Methods and apparatus for processing fluids

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1323622A (en) * 1970-10-02 1973-07-18 Donn Bacchus Ltd Fermentation of fermentable substrates
GB1380316A (en) * 1972-02-24 1975-01-15 British Visqueen Ltd Fermentation
GB1451925A (en) * 1973-09-06 1976-10-06 Ici Ltd Collapsible container
EP0057659A2 (en) * 1981-01-30 1982-08-11 Schering Aktiengesellschaft Draft tube type reactor with centrally arranged auxiliary agitator
EP0071365A2 (en) * 1981-07-23 1983-02-09 Sodastream Limited Improvements in or relating to home brewing
EP0099634A2 (en) * 1982-06-07 1984-02-01 University Of Waterloo Reactor apparatus for multiphase contacting

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1323622A (en) * 1970-10-02 1973-07-18 Donn Bacchus Ltd Fermentation of fermentable substrates
GB1380316A (en) * 1972-02-24 1975-01-15 British Visqueen Ltd Fermentation
GB1451925A (en) * 1973-09-06 1976-10-06 Ici Ltd Collapsible container
EP0057659A2 (en) * 1981-01-30 1982-08-11 Schering Aktiengesellschaft Draft tube type reactor with centrally arranged auxiliary agitator
EP0071365A2 (en) * 1981-07-23 1983-02-09 Sodastream Limited Improvements in or relating to home brewing
EP0099634A2 (en) * 1982-06-07 1984-02-01 University Of Waterloo Reactor apparatus for multiphase contacting

Cited By (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002167A1 (en) * 1988-08-23 1990-03-08 Anidral S.R.L. Kit for the reactivation of dehydrated microbial cultures, particularly of bacteria
US5476763A (en) * 1990-09-28 1995-12-19 Electrolux S.A.R.L. Medical transport assembly
EP0497330A1 (en) * 1991-01-31 1992-08-05 Boehringer Ingelheim Animal Health Inc. Process and apparatus for the surface culture of nucleated cells and cell culture-dependent substances
US5534417A (en) * 1992-06-12 1996-07-09 Ben-Gurion University Of The Negev Microorganism growth apparatus
EP0576870A2 (en) * 1992-06-12 1994-01-05 Ben-Gurion University Of The Negev Research And Development Authority Microorganism growth apparatus
EP0576870A3 (en) * 1992-06-12 1995-04-05 Univ Ben Gurion Microorganism growth apparatus.
US5443985A (en) * 1993-07-22 1995-08-22 Alberta Research Council Cell culture bioreactor
US6391638B1 (en) 1996-09-26 2002-05-21 Metabogal, Ltd. Cell/tissue culturing device and method
WO1998013469A1 (en) * 1996-09-26 1998-04-02 Metabogal Ltd. Cell/tissue culturing device and method
US20110159590A1 (en) * 1996-09-26 2011-06-30 Protalix Ltd. Cell/tissue culturing device, system and method
US6709862B2 (en) 1998-09-01 2004-03-23 The Penn State Research Foundation Growing cells in a reservoir formed of a flexible sterile plastic liner
US6245555B1 (en) * 1998-09-01 2001-06-12 The Penn State Research Foundation Method and apparatus for aseptic growth or processing of biomass
US6432698B1 (en) * 1999-01-06 2002-08-13 Rutgers, The State University Disposable bioreactor for culturing microorganisms and cells
FR2799138A1 (en) * 1999-10-05 2001-04-06 Opifex Disposable reactor for growing micro-organisms, has nested covers defining sealed compartments, pressurized gas stirs and circulates liquid in, and between, compartments
WO2001025394A1 (en) * 1999-10-05 2001-04-12 Bioaccess, S.A. Reactor for culturing cells or micro-organisms or for dissolving or suspending powder in a liquid medium
AU2001262157B2 (en) * 2000-04-03 2005-07-07 Rootec Bioactives Ag Device for cultivating plant or animal tissue cultures
US6794183B2 (en) 2000-04-03 2004-09-21 Rootec Gesellschaft Für Bioaktive Wirkstoffe mbH Device for cultivating plant or animal tissue cultures
DE10016554A1 (en) * 2000-04-03 2001-10-18 Rootec Ges Fuer Bioaktive Wirk Plant or animal tissue cultivation unit, comprises fermenter container, supply for liquid nutrients and gases, discharger for used nutrients, and carrier plates
US8282269B2 (en) 2000-10-09 2012-10-09 Atmi Packaging, Inc. Mixing bag or vessel having a fluid-agitating element
US8182137B2 (en) * 2000-10-09 2012-05-22 Atmi Packaging, Inc. Mixing bag or vessel with a fluid-agitating element
US20100290308A1 (en) * 2000-10-09 2010-11-18 Terentiev Alexandre N Systems using a levitating, rotating pumping or mixing element and related methods
US7762716B2 (en) 2000-10-09 2010-07-27 Levtech, Inc. Mixing vessel with a fluid-agitating element supported by a roller bearing
US7357567B2 (en) 2001-04-10 2008-04-15 Levtech, Inc. Sterile fluid pumping or mixing system and related method
US7942925B2 (en) 2001-07-09 2011-05-17 Surpass Medical Ltd. Implantable intraluminal device and method of using same in treating aneurysms
US6670171B2 (en) * 2001-07-09 2003-12-30 Wheaton Usa, Inc. Disposable vessel
US6844186B2 (en) 2001-07-09 2005-01-18 Wheaton Usa, Inc. Disposable vessel
CN1316013C (en) * 2001-07-09 2007-05-16 阿尔坎包装药物和私人保健公司 Disposable vessel
WO2003006633A1 (en) * 2001-07-09 2003-01-23 Alcan Packaging Pharmaceutical And Personal Care Inc. Disposable vessel
US8419787B2 (en) 2001-11-23 2013-04-16 Surpass Medical Ltd Implantable intraluminal device and method of using same in treating aneurysms
DE10164458A1 (en) * 2001-12-21 2003-07-10 Fraunhofer Ges Forschung Laboratory fermentation vessel, useful for production of phototrophic micro-organisms, comprises thermoplastic foil layers
US8741620B2 (en) 2003-04-27 2014-06-03 Protalix Ltd. Human lysosomal proteins from plant cell culture
US7951557B2 (en) 2003-04-27 2011-05-31 Protalix Ltd. Human lysosomal proteins from plant cell culture
US8449876B2 (en) 2003-04-27 2013-05-28 Protalix Ltd. Human lysosomal proteins from plant cell culture
US8790641B2 (en) 2003-04-27 2014-07-29 Protalix Ltd. Production of high mannose proteins in plant culture and therapeutic uses thereof
US9220737B2 (en) 2003-04-27 2015-12-29 Protalix Ltd. Plant cell culture expressing human lysosomal proteins and uses thereof
US8227230B2 (en) 2003-04-27 2012-07-24 Protalix Ltd. Human lysosomal proteins from plant cell culture
US7992846B2 (en) 2004-01-07 2011-08-09 Atmi Packaging, Inc. Mixing bag with integral sparger and sensor receiver
US8123199B2 (en) 2004-01-07 2012-02-28 Atmi Packaging, Inc. Bioreactor
US7875448B2 (en) 2004-01-12 2011-01-25 Single Use Brx, Llc Bioreactor systems and disposable bioreactor
WO2005067498A2 (en) * 2004-01-12 2005-07-28 Single Use Brx, Llc Bioreactor systems and disposable bioreactor
US8501460B2 (en) 2004-01-12 2013-08-06 Mayfair Technology Limited Liability Company Bioreactor systems and disposable bioreactor
WO2005067498A3 (en) * 2004-01-12 2006-02-16 James Furey Bioreactor systems and disposable bioreactor
US8623640B2 (en) 2004-04-27 2014-01-07 Hyclone Laboratories, Inc. Stirred tank reactor systems and methods of use
US9540606B2 (en) 2004-04-27 2017-01-10 Life Technologies Corporation Stirred tank reactor systems and methods of use
WO2005108546A3 (en) * 2004-04-27 2006-01-19 Baxter Int Stirred-tank reactor system
WO2005104706A3 (en) * 2004-04-27 2005-12-15 Baxter Int Stirred-tank reactor system
WO2005108546A2 (en) * 2004-04-27 2005-11-17 Baxter International Inc. Stirred-tank reactor system
EP1602715A2 (en) * 2004-06-02 2005-12-07 Millipore Corporation Disposable bioreactor
EP1602715A3 (en) * 2004-06-02 2006-04-05 Millipore Corporation Disposable bioreactor
US7629167B2 (en) 2004-06-04 2009-12-08 Xcellerex, Inc. Disposable bioreactor systems and methods
EP1773976B1 (en) 2004-06-04 2016-12-07 GE Healthcare Bio-Sciences Corp. Disposable bioreactor systems and methods
WO2005118771A3 (en) * 2004-06-04 2006-02-02 Michael Fisher Disposable bioreactor systems and methods
WO2005118771A2 (en) * 2004-06-04 2005-12-15 Xcellerex, Inc. Disposable bioreactor systems and methods
DE102004030378A1 (en) * 2004-06-23 2006-02-02 Kroon, Saide Z + L film reactor
US9259692B2 (en) 2005-04-22 2016-02-16 Life Technologies Corporation Gas spargers and related container systems
US9682353B2 (en) 2005-04-22 2017-06-20 Life Technologies Corporation Gas spargers and related container systems
US10328404B2 (en) 2005-04-22 2019-06-25 Life Technologies Corporation Gas spargers and related container systems
US9475012B2 (en) 2005-04-22 2016-10-25 Life Technologies Corporation Gas spargers and related container systems
US8603805B2 (en) 2005-04-22 2013-12-10 Hyclone Laboratories, Inc. Gas spargers and related container systems
US9005971B2 (en) 2005-04-22 2015-04-14 Life Technologies Corporation Gas spargers and related container systems
US7682067B2 (en) 2005-04-22 2010-03-23 Hyclone Laboratories, Inc. Mixing systems and related mixers
WO2007050971A1 (en) * 2005-10-26 2007-05-03 Levtech, Inc. Bioreactor with mixer and sparger
DE102006021984A1 (en) * 2006-05-10 2007-11-15 Sartorius Biotech Gmbh One-way bioreactor for cultivating cells, includes flexible walls, side wall, bottom part and cover part limiting the reactor interior, in which rotation mixer comprising mixing element and mixer shank arranged at the cover part is arranged
DE102006021984B4 (en) * 2006-05-10 2013-05-08 Sartorius Stedim Biotech Gmbh bioreactor
US8846382B2 (en) 2006-08-01 2014-09-30 Oliver Muecke Airlift bioreactor
WO2008014974A1 (en) * 2006-08-01 2008-02-07 Celljet Biotech Limited Liquid circulation system comprising an inflatable guide
US8282267B2 (en) 2006-10-03 2012-10-09 Artelis S.A. Mixing system including a flexible bag, specific flexible bag and locating system for the mixing system
US8292491B2 (en) 2006-10-03 2012-10-23 Artelis S.A. Flexible bag, mixing system and method for fixing a flexible bag inside a rigid container
EP3366762A1 (en) * 2007-05-07 2018-08-29 Protalix Ltd. Large scale disposable bioreactor
WO2008135991A2 (en) * 2007-05-07 2008-11-13 Protalix Ltd. Large scale disposable bioreactor
US10364413B2 (en) 2007-05-07 2019-07-30 Protalix Ltd. Large scale disposable bioreactor
WO2008135991A3 (en) * 2007-05-07 2009-07-02 Protalix Ltd Large scale disposable bioreactor
US9109193B2 (en) 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
EP2020433A3 (en) * 2007-07-30 2011-10-05 Xcellerex, Inc. Continuous perfusion bioreactor system
US9314751B2 (en) 2011-01-07 2016-04-19 Life Technologies Corporation Methods and apparatus for mixing and shipping fluids
US10308907B2 (en) 2011-01-07 2019-06-04 Life Technologies Corporation Systems for inactivating fluid cultures through heating
US9289735B2 (en) 2011-01-07 2016-03-22 Life Technologies Corporation Systems for inactivating fluid cultures through heating
US8608369B2 (en) 2011-01-07 2013-12-17 Hyclone Laboratories, Inc. Methods and systems for heating and mixing fluids
US8961875B2 (en) 2011-01-07 2015-02-24 Life Technologies Corporation Methods for inactivating fluid cultures through heating
US9376655B2 (en) 2011-09-29 2016-06-28 Life Technologies Corporation Filter systems for separating microcarriers from cell culture solutions
US10301585B2 (en) 2011-09-29 2019-05-28 Life Technologies Corporation Filter systems for separating microcarriers from cell culture solutions
US9643133B2 (en) 2011-09-30 2017-05-09 Life Technologies Corporation Container with film sparger
US10350554B2 (en) 2011-09-30 2019-07-16 Life Technologies Corporation Container with film Sparger
US9339026B2 (en) 2012-06-14 2016-05-17 Therapeutic Proteins International, LLC Pneumatically agitated and aerated single-use bioreactor
EP2925427A4 (en) * 2012-11-29 2016-08-17 Emd Millipore Corp 2d low level mixing bag for storage and shipping
US9744507B2 (en) 2012-11-29 2017-08-29 Emd Millipore Corporation 2D low level mixing bag for storage and shipping
US9827541B1 (en) 2012-11-29 2017-11-28 Emd Millipore Corporation 2D low level mixing bag for storage and shipping
US9993785B2 (en) 2012-11-29 2018-06-12 Emd Millipore Corporation 2D low level mixing bag for storage and shipping
WO2014191947A1 (en) * 2013-05-30 2014-12-04 Rootec Bioactives Ag Bioreactor for obtaining secondary plant constituents
EP3004319A4 (en) * 2013-06-05 2017-01-25 GE Healthcare Bio-Sciences AB Disposable container and mixing system comprising the container
GB2522170A (en) * 2013-09-09 2015-07-22 Univ Swansea Bioreactor lid
WO2015077663A1 (en) * 2013-11-21 2015-05-28 Distek Inc. Disposable bioreactors and methods for construction and use thereof
US10059914B2 (en) 2013-11-21 2018-08-28 Distek, Inc. Disposable bioreactors and methods for construction and use thereof
US10399049B2 (en) 2014-03-17 2019-09-03 Advanced Scientifics, Inc. Transportable mixing system for biological and pharmaceutical materials
US9687799B2 (en) 2014-03-17 2017-06-27 Advanced Scientifics, Inc. Transportable mixing system for biological and pharmaceutical materials
US10463571B2 (en) 2014-06-26 2019-11-05 Advanced Scientifics, Inc. Bag assembly and bag system for use with a fluid
US10123940B2 (en) 2014-06-26 2018-11-13 Advanced Scientific, Inc. Bag assembly and system for use with a fluid
US10184105B2 (en) 2015-03-31 2019-01-22 Heliae Development Llc Flexible bioreactor and support structure method
US10184099B2 (en) 2015-03-31 2019-01-22 Heliae Development Llc Flexible bioreactor and support structure system
WO2016161159A1 (en) * 2015-03-31 2016-10-06 Heliae Development, Llc System for vitally supporting organisms and methods of providing and using the same
US10125346B2 (en) 2015-03-31 2018-11-13 Heliae Development Llc Bioreactor sterilization method for multiple uses
US10059918B2 (en) 2015-03-31 2018-08-28 Heliae Development Llc Method of vitally supporting microalgae in a flexible bioreactor
US10047337B2 (en) 2015-03-31 2018-08-14 Heliae Development Llc Method of mixotrophic culturing of microalgae in a flexible bioreactor
US10525425B2 (en) 2015-10-08 2020-01-07 Life Technologies Corporation Methods and apparatus for processing fluids

Also Published As

Publication number Publication date
GB8706739D0 (en) 1987-04-23

Similar Documents

Publication Publication Date Title
Lemberg et al. Cytochromes
Cohn Optimal systems: I. The vascular system
EP2336293B1 (en) Cell culture methods and devices utilizing gas permeable materials
Leach et al. Children's ideas about ecology 1: theoretical background, design and methodology
Koch The surface stress theory of microbial morphogenesis
US4201845A (en) Cell culture reactor
US9845451B2 (en) Multilayered cell culture apparatus
AU723592B2 (en) Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures
Karp Cell and molecular biology: concepts and experiments
AU711724B2 (en) Seeding heart valves
JP4195287B2 (en) Automated bioculture and bioculture experimental system
CA1071556A (en) Cell culture reactor system
Hamer et al. Methane as a carbon substrate for the production of microbial cells
US6576458B1 (en) Cell and tissue culture device with controlled culture fluid flow
JP2007534335A (en) Stirred tank reactor system
Maniloff Nannobacteria: size limits and evidence
US5437998A (en) Gas permeable bioreactor and method of use
AU622921B2 (en) Cell culture apparatus
McLimans et al. Kinetics of gas diffusion in mammalian cell culture systems. I. Experimental
EP0495213A1 (en) Cell culture insert
EP0531631B1 (en) Cell culture apparatus
Talaro et al. Foundations in microbiology
US4717668A (en) Plastic roller bottle for suspension cultures
US20070166819A1 (en) Device for culturing and transporting cells
Boyd et al. Basic medical microbiology