GB2143733A

GB2143733A - Controlled release device comprising a hydrogel

Info

Publication number: GB2143733A
Application number: GB08418421A
Authority: GB
Inventors: Neil Bonnette Graham; Marion Eileen Mcneill; David Anthony Wood
Original assignee: National Research Development Corp UK
Current assignee: National Research Development Corp UK
Priority date: 1983-07-22
Filing date: 1984-07-19
Publication date: 1985-02-20
Also published as: GB2143733B; EP0132384A2; DE3483289D1; US4814182A; EP0132384B1; GB8319766D0; JPH0649644B2; JPS6042321A; GB8418421D0; EP0132384A3

Description

1 GB 2 143 733 A 1

SPECIFICATION

Controlled release device This invention relates to controlled release devices. More particularly, this invention relates to controlled 5 release devices having enhanced duration and/or control of active substance release.

In our GB 2047093B and 2047094B we have described controlled release compositions which comprise an active substance and a polymeric carrier therefor comprising residues having a ratio of number aver age molecular weight to functionality greater than 1,000 which comprise polyethylene oxide and are cross-linked through urethane groups. In many therapeutic and prophylactic medications it can be advan tageous to provide a constant rate of delivery of the medication to the patient, so-called zero order re lease, sustained over a prolonged period of time. A number of active substances have been found to realise this aim when incorporated in controlled release compositions as aforesaid.

However, the release profile of an active substance from an initially dry hydrogel depends, in a com plex manner, on a number of parameters including the physiochernical properties of the active sub stance; in particular, both its solubility characteristics and molecular size in the eluting medium; the geometry of the hydrogel; and the physiochernical properties of the hydrogel; in particular, both its rate of swelling and equilibrium swelling of the device in the eluting medium at the temperature of diffusion.

It has been found that the release profiles of active substances with good solubility in the eluting me- dium, for example an aqueous medium, from initially dry hydrogels, for example hydrogels comprising 20 polyethylene oxide, with an equilibrium swelling of 200 pph or more and of a size suitable for adminis tration are not constant; rather, the release rates are initially large and gradually fall during a less sus tained period of time.

This invention is of particular, but not exclusive, relevance to the treatment of the sexually transmitted disease caused by the virus Herpes Simplex 11. At the present there is no effective therapy for this disease although the virus and/or its replication is known to be destroyed by addition of lithium salts. How ever, lithium salts are both very soluble and are also rapidly excreted from the body.

This invention seeks to provide an improved controlled release device of broad applicability to swella ble hydrogels wherein a greater degree of control over the release profile of an active substance there from is obtained. This invention also seeks to provide effective lithium salt medication, especially in 30 combating Herpes Simplex 11 virus.

According, therefore, to one aspect of this invention, there is provided a controlled release device which comprises:

(i) a hydrogel; and incorporated therewith (Ii) an active substance at least part of at least one surface of the device comprising (iii) a layer which is effectively impermeable to aqueous media.

The hydrogel (i) can comprise a natural or synthetic organic or inorganic material (for example, silica gel). It is preferred, by reason of the greater degree of control which can be achieved by tailoring the hydrogel structure, that the hydrogel is of an organic material. It is also preferred, by reason of the greater reproducibility, that the hydrogel is a synthetic material.

Examples of natural organic materials include crosslinked bio-polymers; for example, crosslinked poly saccharides such as starches, dextrans and celluloses; crosslinked proteins or polypeptides such as colla gen and gelatin.

Suitably the hydrogel comprises a synthetic hydrophilic homo-or copolymer comprising residues deriv- 45 able from at least one of the following monomer classes:

(a) (meth)acrylic acid, (meth)acrylamide, an unsubstituted or hydroxysubstituted, propyl, ethyl, or other alkyl (meth)acrylate or a poly(oxyethylene) (meth)acrylate; (b) a substituted or unsubstituted cyclic mono or poly ether having from 3 to 6 ring atoms or cyclic imine having 3 ring atoms: or (c) a substituted or unsubstituted vinyl alcohol, aldehyde, ether, acetal, ketone, ester, anhydride or sub stituted or unsubstituted N- or C- vinyl heterocyclic compound.

By "(meth)acryl" we mean herein "methacryl" or "acryl" or a copolymer comprising both.

Monomers in class (b) include epoxides such as ethylene oxide, propylene oxide, 1,2-epoxybutane, 2,3 epoxybutane glycidyl ethers, N-(epoxy substituted) heterocyclic compounds such a N-(2,3-epoxyp ropyl)pyrrolidone; epihalohydrins, while not themselves cyclic ethers, do give homo- and copolymers derivable therefrom: examples include epifluorohydron, epichlorohydrin and epibromohydrin. Other cyclicmono- or poly ethers include oxetane, tetra hydrofu ran, dihydropyran, dioxolane and trioxane. Homo- and copoly mers derivable therefrom include partially C, to C, alkyl etherified celluloses and starches, homo-and co poly(alkylene oxides) such as polyoxymethylene, polyethylene glycols and poly-propylene glycols, and 60 polyesters thereof with dicarboxylate acids such as maleic acid which may be cross-linked through reac tion with isocyanate or unsaturated cyclic ether groups.

Monomers in class (c) include methyl and ethyl vinyl ether, methyl vinyl ketone, methallyl alcohol, mal eic anhydride, N-vinyl pyrrolidone, N-vinyl carbazole, C-vinyl pyridine, N-vinyl oxazole, N-vinyl methylox azolidone, vinyl formal, vinyl butyral, vinyl methoxyacetal. Homo- and copolymers derivable therefrom 65 2 GB 2 143 733 A 2 include polyvinyl acetate and polyvinyl alcohol.

The synthetic hydrophilic homo- or copolymer may be chemically crosslinked to form the hydrogel. A general process for accomplishing this in a very satisfactory manner and resulting in a finely divided hydrogel is disclosed in our GB 2090264B. Where the hydrophilic polymer comprises functional groups which comprise an active hydrogen atom (for example, hydroxyi, amino, mercapto, carboxylic or phosphoric acid, amide, thiolic or thionic analogues) chemical cross-linking may be effected by reaction with a di- or poly-isocyanate (such as bis-(4-isocyanato phenyl) methane) or a di- or poly- linear or cyclic olefinically unsaturated ether (such as acrolein tetramer); for example, as disclosed in our GB 2047093B, GB 2047094B and copending application GB 2108517A, from which it will be apparent that where a diiso- cyanate or di-olefinically unsaturated ether is used a reactant comprising at least three active hydrogen 10 atoms must also be present to ensure chemical crosslinking.

Entanglement cross-linking may be utilised, especially where the hydrophilic polymer has a high molecular weight (for example, Rf. greater than 20,000) by incorporating in the hydrophilic polymer and polymerising monomers of functionality greater than two. Examples of such monomers include di- and poly-olefinically unsaturated hydrocarbons, such as divinyl benzene or isoprene, and the di- and poly- 15 olefinically unsaturated esters or ethers, such as acrolein tetramer, triaNyl cyanurate or glycol dimethacrylate.

Block copolymers comprising both hydrophilic and hydrophobic domains (for example, ethylene oxidehigher alkylene oxide, such as propylene oxide, block copolymers) are also very suitable.

Preferably, the hydrogel (i) comprises a homo- or copoly-(alkylene oxide), preferably poly(ethylene ox- 20 ide). It is also preferred that the poly(alkylene oxide) is cross-linked through reaction with isocyanate or unsaturated cyclic ether groups. Very satisfactory examples of such hydrogels, and of their preparation, are disclosed in our aforementioned patents and patent applications.

The present invention is of broad applicability in the formulation of active substances (ii), particularly, but not exclusively, biologically active substances releasable for a sustained period of time. Examples of 25 classes of biologically active substances which may be incorporated in controlled release devices of the present invention include flavourings, pharmaceuticals, bacteriostats, viruscides, pesticides such as insec ticides, nematicides, molluscicides and larvicides, herbicides, fungicides, algaecides, topical or dermato logical agents, antifoulants for marine growth prevention, proteins, for example enzymes, peptides, microbiological and plant hydroculture salts and nutrients and preservatives, veterinary trace metal for- 30 mulations, and other growth promoting factors used in animal husbandry: for example, anti-anaemia preparations and anabolic steroids. Of particular interest is a device of the present invention comprising, as biologically active substance, at least one pharmaceutical.

The devices of this invention thus find wide application in medical and surgical, including veterinary, contexts and in horticulture and agriculture as well as outside these areas.

Specific classes of drug which may be utilised in a controlled release device of the invention include abortifacients such as prostaglandins, hypnotics, sedatives, tranquilisers, anti-pyretics, anti-inflammatory agents, antAistamines, anti-tussives, anti-convulsants, muscle relaxants, anti-tumour agents, for exam pie those for the treatment of malignant neoplasia, local anaesthetics, anti-Parkinson agents, topical or dermatological agents, diuretics, for example those containing potassium, such as potassium iodide, 40 preparations for the treatment of mental illness, for example preparations containing lithium for use in the treatment of manic depression or containing prostaglandins for the treatment of schizophrenia, anti spasmodics, anti-ulcer agents, preparations containing various substances for the treatment of infection by pathogens including anti-fungal agents, for example metronidazole, anti-parasitic agents and other anti-microbials, anti-malarials, cardiovascular agents, preparations containing hormones, for example an45 drogenic, estrogenic and progestational hormones, notably steroids such as oestradiol, sympathomimetic agents, hypoglycaemic agents, contraceptives, nutritional agents, preparations containing enzymes of various types of activity, for example chymotrypsin, preparations containing analgesics, for example as pirin, and agents with many other types of action including nematocides and other agents of veterinary application. Mixtures of active substances may be incorporated into the controlled release device.

The controlled release devices of this invention may be used as a contraceptive device suitably con taining, as active substance, at least one natural or synthetic steroid sex hormone for example an oestro gen or progestogen. Suitably progestogens include the natural progesterone and its synthetic analogues, including 'I l-dehydroprogesterone, delalutin, 21 -fluoro-1 7-acetoxy-6-u- methyl progesterone, medroxypro gesterone acetate, megestrol acetate, chlormadinone acetate, ethisterone, dimethisterone, A-norproges- 55 terone, 19-norprogesterone, 21-norprogesterone, normethandrone, norethynodrel, norethindrone and its acetate, DL- and D-norgestrel, norgestrienone, ethynodiol diacetate, lynstrenol, ethynylestradiol, retropro gesterone, clydrogersterone, norvinodrel, quingestranol acetate, norethisterone and its acetate and oen anthate, anagesterone acetate, medrogestone, clomagestone, allyl estrenol and cingestol, preferably progesterone. Suitably oestrogens include the natural p-oestradiol and its synthetic analogues, principally 60 ethinyloestradiol or mestranol, preferably p-oestradiol.

The controlled release devices of this invention are also useful in the treatment of diabetes and perni cious anaemia where, for example, the controlled release of insulin and cobalamin, respectively, may be utilised.

Moreover, the controlled release devices of this invention are particularly suited to treatment, both pro- 65 3 GB 2 143 733 A 3 phylactic and therapeutic, of tropical diseases; for example malaria, leprosy, schistosomiasis and clonorchiasis. Examples of drugs which can be used as biologically active substance in controlled release devices of this invention for the treatment of these and other tropical diseases include quinine, sulphonamides, rifamycin, clofazimine, thiambutasine, chlorphenyl derivatives, chlorguamide, cycloguanil, pyrimethamine, sulphadiazine, trimethoprim, quinoline derivatives such as pamaquine, chloroquine, pentaquine, primaquine and amocliquine, pararosaniline, sulphamethizole, quinacrine, clapsone, sodium sulphoxone, sulphetrone, sodium hydnocarpate and sodium chaulmoograte. Drugs of particular effectiveness are cycloguanil, pyrimethamine and sulphadiazine.

The controlled release devices of this invention are also very well suited to veterinary applications.

Examples include preparations of antibiotics for general antibacterial activity and also in the treatment of 10 anaplasmosis in cattle; preparations for provision of a wide spectrum of activity against both ectoparasites, for example termites and endoparasites including arthropods, arrested larvae stages of nematodes, lungworms and general strongyles: these may comprise avermectins; preparations for provision of activity against tremotode, cestode and roundworm infections: these may comprise amoscanate and prazi- quantel: preparations for provision of activity against theileria in cattle: these may comprise biologically 15 active naphthoquinones such as menoctone; preparations for provision of activity against babesiosis in cattle, horses and dogs: these may comprise berenil, amiclocarb and diampron; preparation for provision of activity against liver fluke in sheep and cattle and against Haemonchus species: these may comprise closantel.

In accordance with a particularly preferred feature of this invention there is provided a controlled re- 20 lease device for the treatment of the Herpes Simplex 11 virus wherein the active substance is a lithium compound, preferably a lithium salt, especially a soluble lithium salt such as a lithium halide, for exam ple lithium chloride.

In accordance with another preferred feature of this invention, there is provided a controlled release device for release of prostaglandins. Such controlled release devices can have a variety of effects on the 25 body; for example, they may be useful in the treatment of schizophrenia, particularly PGE,. They are, however, or particular interest in their action upon the female reproductive system of both human and non-human animals. By that action the controlled release compositions of this invention have found use as abortifacients; in the induction of labour; in a contraceptive role; and in the treatment of cervical in competence, particularly in administration prior to artificial insemination in non-human animals. Both naturally - occuring and synthetic analogues of prostaglandins are of interest.

The natural prostaglandins of importance in reproduction are those of the E and F groups (for example PGE, PGF,,, PGE, PGF, PGE, and PGF,,), PGI, (prostacyclin) the compounds PGE, and PGF, being of particular interest. Examples synthetic prostaglandins (analogues) include particularly derivatives of PGE, and PGF, specific derivative of clinical promise being 15-methyl-PGF,, PGF,,, 16, 16-dimethyl-PGE,, and 35 also 16, 16-dimethyl-PGE, parabenzaldehyde sernicarbazone ester, 16- phenoxy-17, 18, 19, 20-tetranor PGE2 and especially 16, 16-dimethyl- trans A 2-PGE,which may very suitably be formulated according to the present invention, for example in the form of an ester such as the methyl ester. Particular prostaglan dins may of course be of especial interest for particular applications so that PGE21 for example, is of more interest in the induction of labour whilst 16, 16-dimethyl trans 'L 2-PGE, is of more interest in inducing abortion. It will be appreciated that the invention will be applicable to subsequently prepared novel pros taglandin derivatives having similar but modified properties, for example greater potency, prolonged ac tion, greater specificity, to those at present known and used as abortifacients. Thus, for example, there is considerable interest in "luteolytic" prostaglandins such as 16-(3trifluoromethylphenoxy)-17, 18, 19 20 tetranor- PGF,, and their use in a contraceptive role. It will be appreciated that, if desired, the polymeric 45 carrier may incorporate two more prostaglandins.

By "incorporated therewith" is meant herein that the active substance (ii) may be homogeneously or inhomogeneously dispersed throughout the hydrogel (i) or may be contained in a reservoir within the hydrogel or, indeed, both.

The controlled release devices of the invention may be formed in a variety of configurations; for exam- 50 ple, one which is as a rod, slab, film, hemisphere or is of a hollow or periodic, for example sinusoidal, profile.

The impermeable layer (iii) is preferably hydrophobic; it is preferably also impermeable to the active substance (ii). Suitably, the impermeable layer (iii) covers all of at least one surface, preferably a curved surface, of the device thereby defining at least one orifice thereon through which the hydrogel M is con- 55 tactable by aqueous media. Where the device is formed as a rod, slab or film the impermeable layer may cover substantially all of the surfaces except one or both end profiles (that is the surfaces usually, but not necessarily, substantially planar and of smallest area) the or each of which end profiles defines an orifice.

Such a device, especially one comprising only one orifice, has a much longer half-life of release than like devices without the impermeable layer. Where the device is formed with a single or bis-sinusoidal profile 60 along its length (the first sinusoidal profile may be reflected or out of phase with the second) the im permeable layer may cover substantially all of the surfaces except one or both end profiles. Such a de vice, especially one comprising only one orifice, exhibits a pulsatile release rate. Where the device is formed as a hemisphere, the impermeable layer can cover substantially all of the surfaces except a polar orifice in which case the device will provide a very prolonged release, the rate of which will either in- 4 GB 2 143 733 A 4 crease or remain constant with time. Where the entire curved surface of such a device is covered by the impermeable layer with the equatorial surface uncovered as the orifice the rate of release decreases with time.

The device may also be formed as a ring of hydrogel with the impermeable layer covering all the sur- faces except the internal, typically cylindrical, surface which acts as the orifice. Such a device can provide a longer half-life of release and exhibit a rate of release which is constant or increases with time.

Furthermore, a multiplicity of orifices, for example, in a layer which substantially completely covers the device which may be formed as a rod enables a very precise regulation of half-life of release.

All of the aforementioned examplary devices may be produced by simple technology. Thus, hydrogel 1() for the cylindrical and slab devices may be moulded or continuously produced by reactive extrusion. The 10 hydrogel may then be dip coated (a plurality of coats may be provided) in an organic solution of a thermoplastic elastomer or sleeved with a preformed silicone, natural or synthetic polyclienes or butyl elastomers and then sliced to the desired dimensions, the slicing exposing one or two orifices. The sleeving may be facilitated by incorporating a swelling agent in the sleeve or, in a preferred embodiment espe- cially in relation to rods or slabs, deforming the hydrogel in accordance with our copending application 15 1 1. The hydrogel for the hemispherical and sinusoidal devices may be moulded, coated (prefera bly by dip coating) and cut in an essentially similar manner. The hemispheres may be moulded in tan dem via a sprue which, after coating, is cut to expose a polar orifice in each hemisphere. The orifice may also be formed by lasing the fully coated device. The hydrogel for the ring device may be initially formed as a rod, which is then sliced, coated and, thereafter has an axial hole punched therethrough.

Spray and powder coating technology may also be utilised to form the impermeable coatings which apart from the aforementioned, may comprise drying oils, alkycls, urethanes, acrylics and epoxides. In situ curing may be used, enhanced by thermal, UV or electron beam irradiation in known manner.

The devices of this invention exhibit a much longer half-life of release than like devices without the impermeable layer. This valuable attribute may be used as such or may be utilised to reduce the size of 25 the dosage form, or indeed both. It will be seen, therefore,that the devices are particularly suited to for mulating oral dosage forms.

Comparative example 30 A polyethylene oxide hydrogel was prepared essentially as described in Example 1 of GB.2047093A by 30 reacting 1 mole of polyethylene oxide (9,=8,300) with 0.75 mole of 1,2,6-hexanetriol and 2.125 moles of bis-(4-isocyanatocyclohexyl)methane. The hydrogel was found to have an equilibrium swelling in water, at 20'C, of 400 pph, based on the initial dry weight of hydrogel. Three cylinders of the hydrogel, 6 mm in diameter and 15 mm in length, were cast. The first was sliced 35 into 20 discs each approximately 0.75 mm thick; the second was cut into 6 pieces each approximately 2.5 35 mm thick; and the third was kept intact. The 20 discs, 6 pieces and intact cylinder were next swollen in a 4 mg ml-I LiCI solution and dried. Three size 0 hard gelatin capsules were respectively packed with the 20 discs, 6 pieces and intact cylinder; and capped. To simulate oral dosing, the loaded capsules were separately immersed in 0.01 N hydrochloric acid at 40 37'C and shaken at 200 vibrations per minute in a Grant Shaking Thermostat Bath at 370C. A fourth gela- 40 tin capsule comprising a small amount of methyl violet was also immersed in the hydrochloric acid to ascertain the time to disintegration of the capsule (which was found to be 10 minutes). Aliquots of the hydrochloric acid were removed periodically and their lithium content was determined by atomic absorption spectroscopy (Perkin Elmer 360 atomic absorption spectro photometer). The hydrochloric acid re45 moved was initially replenished by fresh 0.01 N hydrochloric acid at 37'C and, after the first hour, by distilled water at 37C. The results are shown in Figures 1 to 3 of the accompanying drawings. The following Examples illustrate the invention. Examples 1 to 3, inclusive, illustrate the preparation of controlled release reservoir devices of the in- vention in an attempt, successfully realised in Example 3, to secure release of approximately 1 g of lith- 50 ium chloride at a substantially constant rate over a one week period. In all cases where plugs were not retained by means other than friction, the high osmotic pressure expelled them, vitiating the experiments (not examplified).

Example 1

Two polyethylene oxide hydrogels were prepared essentially as described in Example 1 of GB.2047093B by reacting, in a first case, 1 mole of polyethylene oxide (M, =3,207) with 1 mole of 1,2,6 hexanetriol and 2.5 mole of bis-(4-isocyanatocyclohexyl) methane; and, in a second case, 1 mole of the same polyethylene oxide with 2 moles of 1,2,6-hexanetriol and 4 moles of bis- (4-isocyanatocyclohexyl)methane.

2 moles of of 1,2,6-hexanetriol and 4 moles of bis-(4isocyanatocyclohexyl)methane.

A blank hollow cylinder of the second hydrogel, 12 mm in external diameter, 6 mm in internal diame ter, 3 mm in basal thickness and 30 mm in length, was cast. An internal annular groove was then ma chined from the cylinder near its mouth and its cavity was packed with 1 g of lithium chloride crystals.

The first hydrogel was next cast as a plug mating with the annular groove and which, due to its higher swelling, would become tighter on swelling. The resulting cylindrical reservoir was then sheathed with 65 GB 2 143 733 A tight-fitting silicone rubber tubing ("Silescol" translucent silicone rubber tubing ex Esco (Rubber) Ltd.) which had a bore of 8 mm and a wall thickness of 1.6 mm. The tubing was first softened by swelling in diethyl ether for 5 minutes whereupon it was readily possible to insert the hydrogel cylinder into the swollen tube. Thereafter, the diethyl ether was expelled by rapid evaporation in a laminar flow cabinet and then in vacuo. The silicone rubber was 8 mm longer than the cylinder to keep the curved wall of the cylinder covered during swelling; it also contained 16 small (about 2.0 mm in diameter) holes linearly punched therein and evenly spaced apart.

The controlled release reservoir device so formed was immersed in distilled water at 37'C in the thermostated shaking bath at the same temperature in order to avoid a build-up of a static layer of lithium chloride around the device. Aliquots of the water were removed periodically and their lithium content 10 was determined by atomi absorption spectroscopy.

The results are shown in Figure 4 of the accompanying drawings.

Example 2

A size 0 hard gelatin capsule was packed with lithium chloride crystals; capped; and suspended axially 15 and centrally by a nylon thread in a polyethylene vial, 12 mm in internal diameter and 55 mm in length, which acted as a mould. An amount of the second reactant micture mentioned in Example 1 as then cast into the mould around the capsule. The cylinder (12 mm x 50 mm) was removed from the mould by making radial ly-opposed, axial cuts in the via[ and stripping the vial therefrom; and next sheathed with tight-fitting silicone rubber tubing as described in Example 1, except that it contained only 8 small holes. 20 The controlled release reservoir device so formed was then tested as described in Example 1 and the results were shown in Figure 5 of the accompanying drawings.

Example 3

Two size 0 hard gelatin capsules were packed with a total of 1 9 lithium chloride crystals; capped; and 25 inserted into a blank, hollow cylinder of the second hydrogel as described in Example 1. A separately cast plug of the first hydrogel in Example 1 was inserted in the cylinder and retained by a steel pin radi ally driven through both cylinder and plug. The cylinder was then sheathed with tight-fitting silicone rub ber tubing as described in Example 2.

The controlled release reservoir device so formed was then tested as described in Example 1 and the 30 results were shown in Figure 6 of the accompanying drawings.

Example 4 illustrates the preparation of a controlled release reservoir device comprising a hydrogel of a lower equilibrium swelling in water than is the case in Example 1 to 3 and wherefrom plugs are found not be expelled on swelling.

Example 4

A polyethylene oxide hydrogel was prepared essentially as described in Example 1 by reacting 1 mole of polyethylene oxide (9,=3,330) with 4 moles of 1,2,6-hexanetriol and 6 moles of bis-(4-isocyanatocy clohexyl) methane. The hydrogel was cast to form a blank, hollow cylinder 12 mm in external diameter, 6 mm in internal diameter, 4 mm in basal thickness and 50 mm in length. The cylindrical cavity was then 40 packed with 1 g lithium chloride crystals and a plug of the same hydrogel 6 mm in diameter and 6 mm in length was tightly inserted. The resulting cylindrical reservoir was then sheathed as in Example 2.

The controlled release reservoir device so formed was immersed in distilled water at 37'C in a Grant Shaking Thermostat Bath. The release medium was removed daily (being replaced by distilled water at 36'C) for determination of its lithium content by atomic absorption spectroscopy.

The results are shown in Figure 7 of the accompanying drawings. It will be seen that lithium first appeared in the release medium on day 3; thereafter a uniform release rate is established in day 4 which persists into day 9 by which time 70% of the total lithium chloride had been released.

Example 5

A polyethylene oxide hydrogel was prepared essentially as described in Example 1 by reacting 1 mole of polyethylene oxide (Mn=8,400) with 0.65 mole of 1,2,6-hexanetriol and 2.125 moles of bis-(4-isocyana tocyclohexyl)methane. The hydrogel was found to have an equilibriumswelling (weight gain) in water, at 37'C, of 292 pph.

The hydrogel was cast to form a mass 10 mm x 30 mm x 40 mm which was then cut into slabs each 55 mm x 30 mm x 1.35 mm which were next trimmed to provide slabs each 10 mm x 22 mm x 1.35 mm and whose profiles had rounded ends. The slabs were swollen in a 0.1 M p-aminobenzoic acid aqueous solution containing 0.1 M ammonia at 37'C. After equilibrium was attained the swollen slabs were dried at room temperature under vacuum to constant weight. A 10% w/v solution of a thermoplastic rubber (Cariflex TR-1 101 ex Shell Chemicals) was next prepared in chloroform. The dried slabs were dip 60 coated in this solution; dried in air at room temperature; and the process was repeated as required until a tough, water-i m permeable coating had accreted. The coated slabs were then cut to 20 mm lengths removing the semi-circular coated ends and leaving both ends of 10 mm breadth and 1.35 thickness un coated to form a controlled release device. Typically each such device weighed about 300 mg and con tained about 12.0 mg of p-aminobenzoic acid.

6 GB 2 143 733 A 6 The controlled release device so formed was immersed in 1000 mi of a simulated intestinal fluid (6.8 g KH,PQ,, 38 mi 1 M NaOH per litre, adjusted to pH 7.5) in metal baskets which were rotated at 100 rpm. The release of p-aminobenzoic acid was determined by measuring the increase in the ultra-violet absorption at 264 mm at hourly intervals.

The results are shown in Table 1.

TABLE 1

Release of jo-aminobenzoic acid from coated hydrogel devices of slab geometry a Uncoated control b 3 Coats c 5 Coats Time % release Cumulative % Release Cumulative % Release Cumulative (Hours) per hour Release t1lo) per hour Release P/6) per hour Release (0/6) 1 75 75 13 13 10 10 15 2 25 100 14 27 10 20 3 - 100 16 43 12 32 4 - 100 12 55 11 43 - 100 13 68 11 54 6 - 100 9 77 9 63 20 7 - 100 4 81 7 70 Example 6

Hydrogel slabs prepared essentially as described in Example 5 were swollen in a 0.40/6 w/v solution of benzocaine in chlorofom and dried at room temperature under vacuum to constant weight. The dried 25 slabs were then dip coated as described in Example 5. Typically, each such controlled release device contained about 9.0 mg of benzocaine.

Release and determination procedure was essentially as described in Example 5; the results are shown in Table 2.

TABLE 2

Release of benzocaine from coated hydrogel devices of slab geometry a Uncoated control b 3 Coats 35 Time % Release Cumulative % Release Cumulative (Hour) per hour Release t%) per hour Release (OW 1 45 45 7 7 2 31 76 10 17 40 3 12 88 10 27 4 6 94 9 36 3 97 8 44 6 1 98 8 52 6 0 98 8 60 45 Example 7

In this Example the variation of release with the length of controlled release devices essentially as de scribed in Example 5 is described. Hydrogels slabs prepared, swollen and dried essentially as described in Example 5 were then given three dip coatings and cut to lengths to 6 mm, 10 mm or 20 mm. Typically, 50 such a controlled release device 20 mm in length contained about 140 mg of p-aminobenzoic acid.

Release and determination procedure was essentially as described in Example 6; the results are shown in Table 3.

7 TABLE 3

Effect of length of coated device on duration of release GB 2 143 733 A 7 Coated 0. 6 cm Coated 1.0 cm Coated 2. 0 cm 5 Amount Amount Amount released released released (M9) % released (M9) % released (M9) % released 1 9.7 20 9.8 14 10.1 7 10 2 19.2 42 18.3 27 19.3 14 3 27.0 59 28.1 41 29.1 21 4 29.8 65 33.9 50 35.3 25 33.0 72 38.5 57 39.9 28 6 34.7 76 42.2 62 43.8 31 15 7 37.5 82 45.8 67 47.2 38 Example 8

A polyethylene oxide hydrogel was prepared essentially as described in Example 1 by reacting 1 mole of polyethylene oxide ffin=7,000) with 2 moles of 112,6-hexanetriol and 4 moles of bis-(4-isocyanatocyclohexyl)methane. The hydrogel was found to have an equilibrium swelling (weight gain) in water, at 37'C, of 215 pph. The hydrogel was cast to form a cylinder 500 mm in length and 6 mm in diameter. The cylinder was then swollen and dried essentially as described in Example 5, and cut into 10 mm lengths.

Some of these lengths remained, as controls, uncoated; some were given four dip coatings as described in Example 5 with one or both flat ends of the coating cut away; some were sheathed with silicone rub- 25 ber tubing as described in Example 1 but cut to the length of the device and free from punched holes.

Release and determination procedure were essentially as described in Example 5; the results are shown in Table 4.

TABLE 4 30

Release of jo-aminobenzoic acid from coated hydrogel devices of cylindrical geometry c Covered with b 4 coats both ends silicone tubing d 4 coats one end 35 a Uncoated control uncoated both ends exposed uncoated Time % Released Cumulative % Released Cumulative % Released Cumulative % Released Cumulative (hour) per hour release (%) per hour release (%) per hour release (%) per hour release N 1 28 28 16 16 13 13 7 7 40 2 15 43 11 27 8 21 5 12 3 11 54 9 36 7 28 5 17 b4 4 9 63 6 42 5 33 3 20 5 7 70 7 49 5 38 4 24 45 6 5 75 5 54 4 42 3 27 7 5 80 6 60 5 46 3 30 24 - 99 - 95 - 76 67 Example 9

A polyethylene oxide hydrogel was prepared essentially as described in Example 1 by reacting 1 mole of polyethylene oxide (gn=7,000) with 2 moles of 112,6-hexanetriol and 4 moles of bis-(4-isocyanato-cy clohexyl)methane. The hydrogel was cast to form a cylindrical mass 12 mm in diameter from which a number of discs 5 mm in height were cut. The discs were then swollen in a 0.1 M p-aminobenzoic acid aqueous solution containing 0.1 M ammonia at 37'C. After equilibrium was attained (194% weight gain) 55 the swollen slabs were dried at room temperature under vacuum to constant weight.

A cylindrical hole 3.2 mm in diameter was next punched axially through the centre of each disc to produce an annular mass into the pentral channel of which a well-fitting steel rod was introduced. The annular masses were then each dip coated five times in a solution as described in Example 5. The steel rod was carefully removed leaving an annular controlled release device coated on the entire external 60 surface but with an uncoated central channel 3.2 mm in diameter therethrough.

Release and determination procedures were essentially as described in Example 5; result as shown in Table 5.

8 GB 2 143 733 A 8 TABLE 5

Release of p-aminobenzoic acid from externally coated devices of annular geometry a Uncoated control b Coated devices Time % Release Cumulative % Release Cumulative (hour) per hour release P/.) per hour release (0/6) lo 1 17 17 3.5 3.5 10 2 14 31 3 6.5 3 11 42 3 9.5 4 10 52 3.5 13 9 61 2 15 6 7 68 3.5 18.5 15 7 6 74 2.5 21 8 5 79 3 24 Example 10

The preparation, release and determination procedures of Example 5 were followed except that the 20 slabs were swollen in a 2% w/v aqueous solution of caffeine at 37'C the results are shown in Table 6.

TABLE 6

Release of caffeine from coated hydrogel devices of slab geometry 25 Cumulative release (1/6) a Uncoated control b 3 Coats c 2 Coats Time (hour) 20 mm 10 mm 5 mm 8 mm 4 mm 30 1 75 11 18 28 26 47 2 96 20 35 55 48 76 3 - 28 53 70 65 90 4 - 36 72 81 76 97 35 - 42 81 87 83 - 6 - 47 86 91 87 7 - 52 90 94 91 Length of dried controlled release device.

This Example shows clearly the effect of the mechanical properties of the layer; thus, the more coats the more does the layer restrict the swelling of the hydrogel and items reduced the release of active substance.

Example 11

A polyethylene oxide hydrogel was prepared essentially as described in Example 1 by reacting 1 mole of polyethylene oxide (9,=4,360) with 1 mole of 1,2,6-hexanetriol and 2.5 moles of bis-(4-isocyanatocyclohexyl)methane. The hydrogel was cast to form a cylindrical mass 6 mm in diameter from which cylindrical masses 5 mm or 10 mm in height were cut. The cylinders were then swollen in a 2% w/v aqueous EiO solution of sodium jo-aminobenzoate at 37'C. After equilibrium was attained (191% weight gain) the swol- 50 len cylinders were dried at room temperature under vacuum to constant weight.

Each such cylinder was next dip coated five times to produce a cylindrical controlled release device coated on its curved surface but with uncoated ends.

Release of the p-aminobenzoate was followed continuously by LIV spectrometry. The results are shown in Table 7.

9 TABLE 7

Release of jo-aminobenzoic acid from coated hydrogel of devices cylindrical geometry GB 2 143 733 A 9 a. Uncoated control b. Coated 5 mm rod 10 mm rod Time Cumulative % Release per Cumulative % Release per Cumulative Release per (hour) release (%) 2 hr interval release (%) 2 hr interval release M6) 2 hr interval 10 2 27.3 27.3 20.3 20.3 11.3 11.3 4 43.2 15.9 32.2 11.9 17.6 6.3 6 56.1 12.9 42.4 10.2 22.6 5.0 8 66.2 10.1 51.8 9.4 27.1 4.5 10 74.8 8.6 61.0 9.2 30.7 3.6 15 12 80.6 5.8 68.6 7.6 34.3 3.6 14 84.9 4.3 76.2 7.6 37.1 2.8 16 87.8 2.9 82.3 6.1 40.3 3.2 18 89.9 2.1 86.9 4.6 42.7 2.4 20 91.0 1.1 90.2 3.3 45.2 2.5 20 22 - 93.1 2.9 47.6 2.4 24 - 96.0 2.9 49.6 2.0

Claims

1. A controlled release device which comprises:

(i) a hydrogel; and incorporated therewith (ii) an active substance, at least part of at least one surface of the device comprising (iii) a layer which is impermeable to aqueous media.

2. A controlled release device according to Claim 1 wherein the hydrogel (i) comprises a hydrophilic homo- or copolymer comprising residues derivable from at least one of the following monomer classes:

(a) (meth)acrylic acid, (meth)acrylamide or an unsubstituted or hydroxysubstituted alkyl (meth)acrylate or a poly(oxyethylene)(meth)acaylate; (b) a substituted or unsubstituted cyclic mono or poly ether having from 3 to 6 ring atoms or cyclic 35 imine having 3 ring atoms; or (c) a substituted or unsubstituted vinyl alcohol, aldehyde, ether, acetal, ketone, ester, anhydride or sub stituted or unsubstituted N- or C- vinyl heterocyclic compound.

3. A controlled release device according to Claim 1 or 2 wherein the hydrogel (i) comprises a homo- or copoly(alkylene oxide).

4. A controlled release device according to Claim 3 wherein the hydrogel (i) comprises a poly(ethylene oxide).

5. a controlled release device according to Claim 3 or 4 wherein the poly(alkylene oxide) is crosslinked through reaction with isocyanate or unsaturated cyclic ether groups.

6. A controlled release device according to any preceding claim wherein the active substance (ii) cOm- 45 prises a lithium salt.

7. A controlled release device according to any preceding claim wherein the active substance (H) comprises a prostaglandin.

8. A controlled release device according to any preceding claim wherein the active substance (ii) is homogeneously or inhomogenously dispersed throughout the hydrogel (i).

9. A controlled release device according to any preceding claim wherein the active substance (ii) is contained in a reservoir within the hydrogel (i).

10. A controlled release device according to any preceding claim which is formed as a rod, slab, film hemishpere and/or is of hollow or sinusoidal profile.

11. A controlled release device according to any preceding claim wherein the impermeable layer (iii) 55 is hydrophobic.

12. A contolled release device according to any preceding claim where the impermeable layer (iii) is also impermeable to the active substance 'ii).

13. A controlled release device according to any preceding claim where the impermeable layer (iii) covers all of at least one surface of the device thereby defining at least one orifice thereon through which 60 the hydrogel (i) is contactable by aqueous media.

14. A controlled release device according to any preceding claim which comprises a rod, slab or film of hydrogel (i) wherein the impermeable layer (iii) covers all of the surfaces except one or both end pro files.

15. A controlled release device according to any preceding claim wherein the rod or slab of hydrogel 65 GB 2 143 733 A (i) is formed with a single or bis-sinusoidal profile along its length.

16. A controlled release according to any of Claim 1 to 13 which comprises a hemisphere of hydrogel (i) wherein the impermeable layer (iii) covers all of the surfaces except a polar orifice.

17. A controlled release device according to any of Claims 1 to 12 which comprises a ring of hydrogel (i) wherein the impermeable layer (iii) covers all of the external surfaces.

18. A controlled release device according to any of the preceding claims comprising a plurality of orif ices.

Printed in the UK for HMSO, D8818935, 12184, 7102. Published by The Patent Office, 25 Southampton Buildings, London, WC2A lAY, from which copies may be obtained.

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