GB2063253A

GB2063253A - Triazole derivatives processes for their production and pharmaceutical compositions containing them

Info

Publication number: GB2063253A
Application number: GB8034046A
Authority: GB
Original assignee: Glaxo Group Ltd
Current assignee: Glaxo Group Ltd
Priority date: 1979-10-22
Filing date: 1980-10-22
Publication date: 1981-06-03
Also published as: EP0029303A1; AU6360280A; DE3070073D1; AU539189B2; EP0029303B1; GB2063253B

Description

1 GB 2 063 253 A 1

SPECIFICATION Heterocyclic Derivatives

This invention relates to novel heterocyclic derivatives having action on histamine receptors, to processes for the preparation thereof, to pharmaceutical compositions containing them and to their use 5 in therapeutics.

Certain novel heterocyclid derivatives have now been found which have potent activity as H27 antagonists. These compounds, which are more particularly described below, for example show inhibition of the secretion of gastric acid when this is stimulated via histamine receptors (Ash and Schild, Brit. J. Pharmacol. Chemother, 1966, 27, 427). Their ability to do so can be demonstrated in the perfused rat stomach using the method described in German Offeni egu ngssch rift No. 2,734,070, 10 modified by the use of sodium pentobarbitone (50 mg/kg) as anaesthetic instead of urethane, and in conscious dogs equipped with Heidenhain pouches using the method described by Black et al, Nature 1972 236, 385. Furthermore the compounds antagonise the effect of histamine on the contraction frequency of isolated guinea pig right atrium but do not modify histamine induced contractions of isolated gastro-intestinal smooth muscle which are mediated via Hi- receptors.

Compounds with histamine H2-blocking activity may be used in the treatment of conditions where there is an advantage in lowering gastric acidity particularly in gastric and peptic ulceration, as a prophylactic measure in surgical procedures, and in the treatment of allergic and inflammatory conditions where histamine is a known mediator. Thus they may be used for example, either alone, or in combination with other active ingredients in the treatment of allergic and inflammatory conditions of 20 the skin.

The present invention provides compounds of the general formula (1) R3 1 N-N R1R2N-AIK-0-(CH2)nX (CH2)m NH R4 and physiologically acceptable salts, hydrates and bioprecursors thereof, in which Ri represents a heteroaralkyl group or a C,,_1. straight or branched saturated or unsaturated 25 alkylene chain optionally substituted by a hydroxy group or R, represents a Cl-,, straight or branched saturated or unsaturated alkylene chain substituted by an ester group C02R. or an amide group CONH2.

where R. is a C,-, alkyl group, or R1 represents a Cl-C,, straight or branched saturated or unsaturated alkylene chain interrupted by an oxygen atom, a sulphur atom, a sulphoxide group, a sulphone group, an amide group (-CONH- or-NHCO-) or an ester group (-CO-O- or -0-CO), with the provisos that Rl does not represent a Cl-, alkyl group substituted by a Cl-, alkoxy group and that when the alkylene chain is interrupted by an oxygen or sulphur atom or a sulphoxide, amide (CONH) or ester (COO) group there must be at least two carbon atoms between that group and the nitrogen atom to which Ri is attached; R represents hydrogen or a Cl-4 alkyl group; Alk represents a straight or branched alkylene chain of 1 to 6 carbon atoms, preferably 1 to 4 carbon atoms; Q represents a furan or thiophen ring in which incorporation into the rest of the molecule is through bonds at the 2- and 5-positions, the furan ring optionally bearing a further substituent R. 40 adjacent to the group R1R2NAlk-, or Q represents a benzene ring in which incorporation into the rest of the molecule is through bonds at the 1 - and 3- or 1 - and 4-positions; R6 represents halogen, or Cl4 alkyl which may be substituted by hydroxy or Cl-4 alkoxy; X represents -CHI--, -0-, -S-, or -NH-; n represents zero, 1 or 2; m represents 2, 3 or 4; R3 represents hydrogen, alkyl, alkenyl, aralkyl, C2-6 alkyl substituted by hydroxy or alkoxy; and R4 represents hydrogen, alky], alkenyi, aralkyl, hydroxyalkyl, acyloxyalky], alkoxyalkyl, aryloxyalkyl, aralkyloxyalkyl, aminoalkyl, alkylaminoalkyl, dialkylaminoalkyl, hydroxy or alkoxy or the group NR7R.

where R7 represents hydrogen, alkyl, alkyl substituted by hydroxy or Cl-3 alkoxy, alkenyl, aralkyl or heteroaralkyl and R. represents any of the groups defined for R 7 or may represent the group COR, where R. represents hydrogen, alkyl, aryl, aralkyl, alkoxy, heteroary] or monocyclic heteroaralkyl or R, represents the group SOA, where RIO represents alkyl or aryl, or R, represents the group C-NHR1, 55 where Y is oxygen or sulphur and 11 Y GB 2 063 253 A 2 R11 represents hydrogen, alkyl, cycloalkyl, aryl or aralkyl, or R7 and R. taken together may represent the group =CRUR,,, where Ri. represents aryl or heteroaryl and 11,3 represents hydrogen or alkyl with the proviso that when X is -NH-then n is zero.

The term "alkyi" as a group or part of a group means that the group is straight or branched and has unless otherwise stated preferably 1 to 6 carbon atoms, and in particular 1 to 4 carbon atoms, e.g. methyl or ethyl and the term "alkenyl" means that the group has preferably 3 to 6 carbon atoms. The term "eycloalkyi" means that the group has 3 to 8 carbon atoms. The acyl portion of an acyloxyalkyl group means an aroyl, aralkanoyl or Cl-, alkanoyl group. Examples of acyloxyalkyl groups include acetoxymethyl, formyloxymethyl, benzyloxymethyl and phenylacetoxymethyl. The term "unsaturated- in relation to the alkylene chain within R, means that the chain contains at least one -C=C- or --C=-C- grouping. The term "aryl" as a group or part of a group preferably means phenyl or substituted phenyl, for example phenyl substituted with one or more Cl-, alkyl or C,-, alkoxy groups or halogen atoms, e.g. fluorine. The term -heteroaryl- as a group or part of a group within the definition of R7 and R. generally means a 5 or 6 membered monocyclic unsaturated ring which may contain one or more heteroatoms selected from oxygen, nitrogen and sulphur, e.g. furyl, pyridyi, thiazolyl, or thieny]. The 15 term -heteroaralkyl- as applied to the group R, means that the group is made up of a heterocyclic part which is a monocyclic or bicyclic unsaturated ring which may be unsubstituted or substituted, and an alkyl part which is a straight or branched C1-4 alkyl chain. The ring may contain from 5 to 10 atoms selected from carbon, oxygen, nitrogen or sulphur and the ring is linked to the alkyl part either through carbon or nitrogen. When the ring is substituted the substituent is selected from Cl-, alkyi, C1-3 alkoxY, 20 hydroxy, hydroxyalky], aminoalkyl, alkylaminoalkyl, dialkylaminoalkyl and halogen. If the heterocyclic ring is monocyclic it preferably contains 5 or 6 members and if it is bicyclic it preferably contains 9 or members. Examples of such heteroaralkyl groups are those in which the heterocyclic portion is a furyl, thienyl, pyrrolyl, pyridiny], pyrimidiny], triazinyl, oxazolyl, triazoly], thiazoly], isoquinoliny], quinolinyl or indolyl ring and the alkylene portion is a methylene, ethylene or propylene grouping. When R1 represents an optionally substituted alkylene chain this may for example be saturated and contain up to 16 carbon atoms, or be substituted by or interrupted by an optional amide or ester grouping.

The invention includes the compounds of formula (1) in the form of physiologically acceptable salts with inorganic and organic acids. Particularly useful salts include hydrochlorides, hydrobromides, sulphates, methanesulphonates, acetates, maleates, succinates, citrates, tartrates, benzoates and fumarates. the compounds of formula (1) and their salts may also form hydrates, which hydrates are also to be considered as part of the invention. The compounds of formula (1) can exhibit tautomerism and the form is intended to cover all tautomers. Where optical isomers may exist the formula is intended to cover all diastereoisomers and optical enantiomers. The term bioprecursors as used herein means compounds which have a structure different to that of the compounds of formula (1) but which, 35 upon administration to an animal or human being, are converted in the body into a compound of formula (1).

The compounds according to the invention, preferably in the form of a salt, may be formulated for administration in any convenient way and the invention includes within its scope pharmaceutical compositions containing at least one compound according to the invention adapted for use in human 40 or veterinary medicine. Such compositions may be formulated in a conventional manner using one or more pharmaceutically acceptable carriers or excipients. Such compositions may also contain if required other active ingredients, e.g. H,-antagonists.

Thus the compounds according to the invention may be formulated for oral, buccal, topical, parenteral or rectal administration. Oral administration is preferred.

For oral administration, the pharmaceutical composition may take the form of for example, tablets, capsules, powders, solutions, syrups or suspensions prepared by conventional means with acceptable excipients. For buccal administration the composition may take the form of tablets or lozenges formulated in conventional manner.

The compounds of the invention may be formulated for parenteral administration by bolus 50 injection or continuous infusion. Formulations for injection may be presented in unit dosage form in ampoules, or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising and/or dispersing agents. Alternatively, the active ingredient maybe in powder form for reconstitution with a suitable vehicle, e.g. sterile pyrogen-free water before 55 use.

The compounds of the invention may also be formulated in rectal compositions such as suppositories or retention enemas, e.g. containing conventional suppository bases such as cocoa butter or other glyceride.

5.

For topical application, the compounds of the invention may be formulated as ointments, creams, 60 gels, lotions, powders or sprays in conventional manner.

For internal administration a convenient daily dosage regime of the compounds according to the invention would be 1 to 4 doses to the total of some 5 mg to 2 g per day, preferably 5 to 500 mg per day, dependent upon the condition of the patient. Examples of suitable meanings for the groups R,, R21 R3 and R4 areas follows:- 3 GB 2 063 253 A 3 Ri: a heteroaralkyl group in which the heterocyclic portion is a fury], thieny], pyrrolyl, pyridinyl, pyrimidinyi, triazinyl, oxazoly], triazolyl, thiazolyl, isoquinolinyi, quinolinyl or indolyl ring and the alkylene portion is a methylene, ethylene or propylene grouping; alkylene chain which is saturated and contains up to 16 carbon atoms or is substituted by or interrupted by an amide or ester grouping; R2: hydrogen or a methyl or ethyl group; IR,: hydrogen, C,_4 alkyl (e.g. methyl, ethyl or propyl), or hydroxy C2-4 alkyl (e.g. 2-hydroxyethyl); R4: hydrogen, hydroxy, Cl-4 ilkyl (e.g. methyl, ethyl isobutyl), hydroxy C 1-4 alkyl (e.g. hydroxymethy], 2hydroxyethyl or 1 -hydroxy-1 - methylethyl), C,-, alkoxy Cl-4 alkyl (e.g. methoxymethyl or methoxyethyl), phenyl Cl-3 alkyl (e.g. benzyi or phenethyl), C2-4 alkanoyloxy Cl-4 alkyl (e.g. acetoxymethyi), amino Cl-4 alkyl (e.g. aminomethyl), amino, C -4 alkylamino (e.g. methylamino or ethylamino) or di-Cl-4 alkylamino (e.g. dimethylamino, diethylamino or dipropylamino), phenyl C,- , alkylamino (e.g.

benzylamino), or a heteroaryl Cl-, alkylamino group where the heteroaryl ring is 5 or 6 membered and contains one heteroat ' om (e.g. 3- or 4-pyridyl methyl); or the group NHCOR, where R. represents hydrogen, Cl-, alkyl (e.g. methyl or ethyl), Cl-3 alkoxy (e.g. methoxy or ethoxy), furyl, pyridyl, thiazoly], thieny], or phenyl optionally substituted by a Cl-3 alkyl (e.g. methyl) or Cl-3 alkoxy (e.g. methoxy) 15 group; or the group NHS02R10 where R1. represents Cl-3 alkyl (e.g. methyl), or phenyl optionally substituted by a C 1-3 alkyl (e.g. methyl) or Cl3 alkoxy (e.g. methoxy) group; or the group NHCONHR11 where R11 is C, -3 alkyl (e.g. methyl), C.7 cycloalkyl (e.g. cyclohexyl), or phenyl optionally substituted by a C,-3 alkyl (e.g. methyl) or C,-3 alkoxy (e.g. methoxy) group; or the group N=CHR12 where R12 is a phenyl or pyridyl (e.g. 3- or 4-pyridyl) group.

The group Alk may be for example the group (CH2)p where p is 1, 2 or 3.

Q may be for example a benzene ring incorporated into the rest of the molecule through bonds at the 1 - and 3- or 1 - and 4-positions, with n as zero, X as oxygen and m as 3 or 4; or Q may be a furan or thiophene ring with n as 1, X as sulphur and m as 2, with the furan ring optionally containing a further substituent R. where R. is bromine, Cl-3 alkyl (e.g. methyl, ethyl or isopropyl), or Cl-3 alkoxy-Cl-3 alkyl (e.g. methoxymethyi); or Q may be a furan ring with n as 1, X as CH2 and m as 2.

In particular the groups RJ, R21 R3 and R4 may have meanings as follows:

Ri: a heteroaryl methyl group where the heteroaryl ring is 5- or 6membered, contains one heteroarom and is linked to the methyl portion via a carbon atom; or a C11-1. straight or branched saturated alkylene chain; or a Cl-,, straight or branched saturated alkylene chain interrupted by an 30 amide (-NHCO-) group; R2: hydrogen or a methyl or ethyl group; R,: hydrogen, methyl, ethyl or 2-hydroxyethyl; R4: hydroxy, phenyl C,-3 alkyi (e.g. benzyi); Cl_4 alkyl substituted by hydroxy, Cl-3 alkoxy, C24 alkanoyloxy or amino (e.g. hydroxymethyl, 2-hydroxyethyl, acetoxymethyl or aminomethyl); amino, di 35 C,-4 alkylamino (e.g. diethylamino); NHCOR9 where R9 represents hydrogen, Cl-, alkyl (e.g. methyl), Cl-, alkoxy (e.g. ethoxy) or phenyl; NHCONHR11 where Ril represents phenyl, or N=CHR12 where R12 is phenyl or pyridyl (e.g. 4-pyridyl).

Alk is particularly a methylene or ethylene group, more particularly methylene.

Q is particularly a benzene ring incorporated into the rest of the molecule through bonds at the l- 40 and 3-positions, with n as zero, X as oxygen and m as 3 or 4; or a furan ring optionally containing a further-substituent R. where R. is Cl-4 alkyl (e.g. methyl), with n as 1, X as sulphur and m as 2.

Preferably Q is a benzene ring in which incorporation into the rest of the molecule is through bonds at the 1 - and 3-positions, n is zero, X is oxygen and m is 3 or 4. Ri is preferably a heteroaralkyl group in which the alkyl portion is a methylene group.

A preferred group of compounds of formula (1) are those of formula (11) R2 R 14 CH2 NCH2--(::i, R3 1 d-N 0 (CH 2) 3NH /-- R4 N where R14 is a 5- or 6-membered heteroaryl group containing one heteroatom and linked through a carbon atom (e.g. 2-furyl, 2-thieny], 2- pyrrolyl), R2 is hydrogen or methyl; R3 is hydrogen or methyl; and 50 R4 is an amino, hydroxyalkyl, alkanoyloxyalkyl, alkoxyalkyl, aminoalkyl, benzyi, formamido, alkoxycarbonylamino, alkanoylamino, hydroxy, aroylamino, phenylcarbamoyl amino, or pyridyimethylenamino group. Particularly preferred compounds are: 1 -m ethyAM3-[3 [(dodecylamino) methyl] phenoxylpropyll- 1 H-1,2,4triazole-3,5-diamine, 55 Wbutyl-[3-[3-[(1 -methyl-3-amino-1 H-1,2,4triazol-5yi)aminolpropoxylphenyimethylaminolhexanamide, diamine, 1 -m ethyl-N543- [3-[Q-fu ra nyl m ethyl) amino m ethyl] phenoxyl propyl- 1 H-1,2,4-triazole-3,5- 4 GB 2 063 253 A 4 1 -methyl-N'-[3-[3-[(1 H-pyrrol-2-yl-m ethyi)a m!no m ethyl] phenoxyl propyll- 1 H-1,2,4-triazole-3,5 diamine, 5-[[2-[[[5-[[N-m ethyM2-furanyl m ethyi)a m inol methyl]-2-fu ranyll methyllthiol ethyl] am inol- 1 - methyl-1 H-1,2,4-triazole-3-methanol, and their physiologically acceptable salts.

According to one aspect the invention provides compounds of formula (1) in which R1 represents a heteroaralkyl group; and R4 represents NR7R. where R7 and R. each represent hydrogen, alkyl, alkyl substituted by hydroxy or C,-3 alkoxy, alkenyi, aralkyl or heteroaralkyl or R7 and R. together represent =CR,2R,3 where R12 represents aryl or heteroaryl and R13 represents hydrogen or alkyl.

According to another aspect the invention provides compounds of formula (1) in which R, is other than a heteroaralkyl group; and R4 represents NR7R. where R7 and R. each represent hydrogen, alkyl, alkyl substituted by hydroxy or C1-3 alkoxy, alkenyi, aralkyl or heteroaralkyl or R, and R. together represent =CRJ1, where R,, represents aryl or heteroaryl and R 13 represents hydrogen or alky].

It will be appreciated in the methods for the preparation of compounds of formula (1) given below, 15 that for certain reaction steps it may be necessary to protect various reactive substituents in the starting materials for a particular reaction and subsequently to remove the protecting group. Such protection and subsequent deprotection may be particularly pertinent where R, and/or R, in intermediates used to prepare compounds of formula (1) are hydrogen atoms and/or when R, in intermediates is an alkyl group bearing a hydroxy substituent and/or when R4 in certain intermediates is 20 a free amino group. Standard protection and deprotection procedures can be employed: for example formation of phthalimide (in the case of primary amines), benzyl, benzyioxycarbony], or trichloroethoxycarbonyl derivatives. Subsequent cleavage of the protection group is achieved by conventional procedures. Thus a phthalimide group may may be cleaved by treatment with a hydrazine e.g. hydrazine hydrate or a primary amine for example methylamine; benzyl or benzyloxycarbonyl 25 derivatives may be cleaved by hydrogenolysis in the presence of a catalyst, e.g. palladium, and trichforoethoxycarbonyl derivatives may be cleaved by treatment with zinc dust.

In describing the processes which may be used in preparing compounds of formula (1) or intermediates used in the preparation thereof, any of R, to R13, Alk, Q, X, n and m are as defined in formula (1) unless otherwise stated.

Compounds of formula (1) where Alk represents CH2 and in which R4 is other than the group -N=CRUR13 may be prepared by reducing the intermediate formed from reacting a compound of formula (111) R 3 I-N (M) W-O(CH2),X(CH2) m NH--11: N '"4 in which W represents the group -CHO with a reagent R1R2NI-1.

Reaction may be effected by treatment with ammonia or an amine R1R2NH in a solvent e.g. tetrahydrofuran or an alkanol such as ethanol or methanol, followed by reduction e.g. with a hydride reducing agent such as an alkali or alkaline earth metal borohydride e.g. sodium borohydride, or aluminium hydride or lithium aluminium hydride or with hydrogen and a metal catalyst e.g. palladium 40 or platinum.

Alternatively, for the production of compounds of formula (1) in which Alk is Cl-, alkylene group and R4 is other than the group -N=CRUR13. a compound of formula (N) R3 1 N-N R2MAIKO (CH2)n X (CH2)m NH R 4 (M may be reacted with an aldehyde R1CHO under the reducing conditions described above; the group R11 having a meaning such that the resultant RICH2represents a group R, as defined above.

Compounds of formula (1) may also be prepared by reacting an intermediate of formula (M above with an appropriate halo compound such as a heteroaralkyl halide (e.g. 2- furyimethyl chloride).

Compounds of formula (1) in which R4 represents the group N=CRUR1. may be prepared from compounds of formula (1) in which R4 represents NH2 by reaction with an aldehyde or ketone R12R13C0 in a solvent such as benzene, ethanol or methanol. The reaction is preferably carried out with heating, 50 e.g. at reflux.

Compounds of formula (1) in which R4 is an acyloxyalkyl group may be prepared by treating the corresponding hydroxyalkyl compound with an appropriate acid, e.g. acetic or benzoic acid at elevated temperatures e.g. 80-1201C in the absence or presence of a solvent such as toluene.

1 A GB 2 063 253 A 5 Compounds of formula (1) in which R4 is the group NR7R. where R. is -COR., -SOA, or -C(=YMR11 may be prepared by treating an amino triazole (V) R3 1 1 J-N R1 R2NAW-0-(CH 2), X(CH2)m NH ---/--NHR7 (V) in which RJ, R21 R3 and R7 are as defined in formula (1) or are groups readily convertible thereto with a reagent capable of replacing the hydrogen atom in the group NHR7 by the group R. where R. is as 5 defined in formula (1).

Thus for example the aminotriazole (V) may be reacted with an activated derivative of either a carboxylic acid R.COOH or a sulphonic acid Ri.SO3H or the aminotriazole (V) may be reacted with an isocyanate or isothiocyanate R'11NCY in which R'11 has any of the meanings defined for R11 in formula (1) except hydrogen or represents an alkali metal atom such as potassium or sodium or an alkoxycarbonyl group, e.g. ethoxycarbonyl, to give a compound of formula (1) in which R. is respectively the group COR., S02Rj. or C-N H-R 11. il Y Suitable activated derivatives include acid halides e.g. acid chlorides, alkylchloroformates, acid anhydrides including mixed anhydrides (e.g. acetic formic anhydride), or esters such as alkyl esters, 15 ortho esters and (1 -alky]-2-pyridinyi) esters.

The reaction with an acid halide is preferably carried out in the presence of a base e.g. an inorganic base such as sodium hydroxide or an organic base such as triethylamine or pyridine. The reaction with an alkylchloroformate is preferably carried out in the presence of a base, e. g. potassium carbonate or triethylamine, in a solvent such as dimethylformamide. The reaction with an acid anhydride may be carried out in the absence or presence of a solvent such as pyridine.

In the reaction with an isocyanate or isothiocyanate, compounds of formula (1) in which R,, is other than hydrogen are conveniently prepared by carrying out the reaction in a solvent such as acetonitrile at an elevated temperature, e.g. reflux. Compounds of formula (1) in which IR, is hydrogen may be prepared by heating the aminotriazole (V) with an appropriate organic-isocyanate or isothiocyanate such as ethylearbonisothiocyanatidate, at an elevated temperature followed by hydrolysis of the resulting ester, for example with a base such as aqueous ethanolic sodium hydroxide Compounds of formula (1) in which R1 is an alkylene chain interrupted by an amide grouping may be prepared by heating a compound of formula (1) in which R1 is an alkylene chain substituted by an ester grouping C02R. (i.e. R, is the group R.0OC(CH1_) with an amine CH, (CH2),NH2 to give a compound of formula (1) in which R1 is the group CH,(CH2)YNHCO(CH2),-- where x and y are integers and the total of x and y is not greater than 15.

Intermediates of formulae (111) and (]V) may in general be prepared by the methods referred to in British patent specification No. 2023133A and in European patent specification publication No.

0016565.

Where the product of any of the above processes is a free base and a salt is required ' the salt may be formed in a conventional manner. Thus for example, a generally convenient method of forming the salts is to mix appropriate quantities of the free base and the acid in an appropriate solvent(s) e.g. an alcohol such as ethanol or an ester such as ethyl acetate.

The invention is illustrated but not limited by the following Examples and Preparations.

In the Examples temperatures are in OC. Preparative and thin layer (t.l.c. ) chromatography were carried out on silica using, unless otherwise stated, the following solvent system: ethyl acetate:water:isopropanol:0,88 ammonia (25:8A 52).

Preparation 1 N-Butyi-6-aminohexanamide N-Butyl-6- [[(phenyl methoxy)ca rbo nyll am i no] hexa na m ide A stirred solution of 6- [[(phenyl m ethoxy)ca rbonylla m i no] hexanoi c acid (26.53 g) and dimethylformamide (10 drops) in dichloromethane (200 mi) was treated with thionylchloride (22 mi) at room temperature for 17 h. The solution was evaporated and the residue was azeotropically dried with toluene to give a yellow oil. Butylamine (50 mi) was added to a stirred solution of the yellow oil in dichloromethane (200 mO at 00 over 0.5 h and the reaction was stirred at room temperature for 17 h. The solution was washed with water and evaporated to leave a solid which was washed with light petroleum b.p. 60-801 to give the title compound as a cream solid (30 g).

N.m.r. (CDCI,) 2.70, s (5 H); 3.95, brt, (1 M; 4.8, brt, (1 H; 4.95, s, (2 H); 6.8, m, (4 ffl; 7.85, t, (2 H); 8.1-8.9, m (10 H); 9.10, t, (3 H).

26 6 GB 2 063 253 A 6 N-Butyl-6-aminohexanamide Hydrogen, at atmospheric pressure, was passed through a stirred suspension of N-butyl-6[[(phenyl m ethoxy)-carbonylla mino] hexa nam ide (12.8 g) and palladium oxide on charcoal (1 g) in ethanol (150 mi) until uptake of hydrogen ceased. The suspension was filtered and evaporated to give 5 an oil which was partitioned between ethyl acetate (100 mi) and 2 N sodium hydroxide solution (100 mi). The organic extract was extracted with 2 N hydrochloric acid which was basified to pH 12 with 2 N sodium hydroxide solution and extracted with ethyl acetate. The organic extracts were evaporated to give the title compound as a cream semi solid (4 g) which was used without further purification.

Example 1 (a) 1 -Methyi-N-[3-[3-[(3-pyridinylmethyi)aminomethyllphenoxy]propyll-1 H1,2,4-triazole-3,5 diamine A solution of NI-cyano-N-[3-[3-(1,3-diaxolan-2-yi)phenoxylpropyll-1 - methyl-2(phenyl methylene) hydrazinecarboximidamide (3.06 g) in tetrahydrofuran (60 mi) was stirred at room temperature for 0.5 h. with 2 N hydrochloric acid (7 ml). The mixture was treated with 3- aminomethylpyridine (15 mi) and stirred at room temperature for a further 0.5 h. The mixture was treated with sodium borohydride (1.5 g), stirred at room temperature for 18 h diluted with ethyl acetate, filtered and the filtrate evaporated in vacua. The resulting oil was partitioned between ethyl acetate and water. The organic phase was evaporated in vacua and the residue was purified by column chromatography on silica, using methanol. Evaporation of the eluates gave a brown oil which was triturated with diethyl ether to give the title compoundas an off white solid (0.12 g) m.p. 114-7 0, 20 T.I.c. Rf 0.6.

The following compounds were similarly prepared from Nl-cyano-N-[3-[3-(1, 3-dioxolan-2- y1)phenoxyl propyll- 1 -m ethyl-2-(phenyl methylene) hydrazineca rboxi m ida m ide [A] and the corresponding amines.

(b) A (3.06 g) and furfurylamine (15 ml) gave 1 -methyl-N5-[3-[3-[(2 fu ranyl methyi)a m ino methyl] phenoxyl propyll- 1 H-1,2,4-triazole-3,5diamine as a white solid (0.77 g).

m.p. 91.5-93'.

Assay found: C1.HUN.02 requires:

C, 60.45; H, 6.76; N, 23.06; C, 60.66; H, 6.79;N, 23.58% (c) A (3.06 g) and methyl, 11 -aminoundecanoate (4.85 g) gave methyl, 1143-[3-[(1 -methyl-3- 30 amino-1 H- 1,2,4-triazol-5-yi)a m inol propoxyl phenyl methyla minol undeca noate as a white solid (0.89 g) m.p. 83.5-851.

Assayfound: C2.HUN.03 requires:

C, 63.20; H, 8.86; N, 17.56; Q63.26; H,8.92;N,17.71% (d) A (2.2 g) and dodecylamine (10 mi) gave 1 -methyl-N5-[3-[3 [(dodecylam ino) m ethyl] phenoxy] propyl]- 1 H-1,2,4-triazole-3,5- diamine as a white solid (0.5 9) m.p.

85-860.

Assay found: C2.H44N60 requires:

C, 67.05; H, 9.73; N, 18.46; C, 67.56; H, 9.90;N, 18.91 % (e) A (2.03 g) and hexadecylamine (11 g) gave 1 -methyl-N5-[3-[3 [(hexadecyl ami no)m ethyl] phenoxyl propyll- 1 H-1,2,4-triazole-3,5- diamine as a white solid (0.1 g) m.p.

88-90.51, after recrystallisation from a mixture of ethyl acetate and light petroleum b.p. 60-801 0M.

Assayfound: Q68.62; H,10.11; N,16.12; v C29H.52N60. 0.5 H20 requires: C, 68.33; H, 10.48; N, 16.49% 45 (f) A (3.06 g) and methyl-(2-amino)acetate (3 g) gave methyi-[3-[3[(1- methyi-3-amino-1 H-1,2,4 triazol-5-y1)a m inol propoxyl phenyim ethyl am i no] acetate as an oil (590 mg) purified by chromatography on silica using methanol/ethyl acetate (1:1).

T.I.c. silica ethyl acetate:methanol 0M Rf 0.5.

N.m.r. (CDC13) 2.75 t, (1 H); 3.0-3.3, m, (3 H); 5.66, t, (1 H); 5.90, t, (2 H); 6.10, br.s. (2 H); 6.25, 50 s, (2 H); 6.30, s, (3 H); 6.48, q, (2 H); 6.60, s, (2 H); 6.65, s, (3 W; 7.78, br.s, (1 W; 7.88, m, (2 H).

mg).

(g) A (3.06 g) and N-butyi-(6-amino)hexanamide (3.7 g) gave N-butyl43-[3[(1 -methyl-3-amino1 H- 1,2,4-triazol-5-yl)a minol propoxyl phenyl methyla m inol hexana m ide dihydrate as a brown oil (700 GB 2 063 253 A 7 N.m.r. (CDC13) 2.8, t, (1 H); 11-3.4, m, (3 H); 4.4, m, (1 H); 5.6, rn, (1 H); 5.98, t, (2 H); 6.2, br, (2 H), 6.32, s, (2 H), 6.50, q, (2 H); 6.68, s,(3 H); 6.83, q, (2 H), 7. 45, t, (2 H); 7.8-8.1, m, (4 H); 8.2-9.0, rn, (11 H); 9.15, t, (3 H).

Assay found.. C, 57.66; H, 8.20; N, 19.82; C231-129N702. 2 H20 requires: C, 57.48; H, 8.80; N, 20.39% 5 Example 2 (a) 5-[[3-[3-[(3Arnino-1 -m ethyl- 1,2,4-triazol-5yi)a m inol propoxy] phenyl methylla m inomethyllfu ra n-2-metha nol A solution of 1 -methyl-N 5-[3-[3-(aminomethyi)phenoxylpropyll-1 H- 1,2,4- triazole-3,5-dia mine (830 mg) and 2-hydroxymethyifuran-5-carboxaldehyde (380 mg) in ethanol (30 mi) was stirred at 10 room temperature for -6 h and treated with sodium borohydride (1 9) for 16.5 h. The mixture was quenched with water, partially evaporated and extracted with ethyl acetate. The organic extracts were evaporated to give a yellow oil which was purified by preparative layer chromatography to give the title compound as a white solid (400 mg) m.p. 120-1221.

N.m.r. (CDC13/DMSO) 2.79, t, (1 H); 3-3.3, m, (3 H); 3.87, dd, (2 H); 4. 13, t, (1 H); 5.5, s, (2 H); 15 5.70, br.s. (2 1-1); 5.95, t, (2 1-1); 6.27, s, (2 H); 6.29, s, (2 H); 6. 4-6.7, m, (2 H); 6.68, s, (3 H); 7.7 5-8.10, m, (3 H).

The following compounds were similarly prepared from 1 -methyl-NS-[3[3(aminomethyi)phenoxylpropyll-1 H-1,2,4-triazole-3,5-diamine [A] and the corresponding heterocyclic aldehydes.

(b) A (276 mg) and thiophene-2-carboxaldehyde (0. 1 m]) gave 1 -methyl-N 5 -[3-[21-[(2th ienyl m ethyi)a m inom ethyl] phenoxyl propyll- 1 H-1,2,4triazole-3,5-diamine as a white solid (24 mg) m. p. 96.5-81.

Assay found: C, 57.60; H, 6.45; N, 22.27; C1.1---124N.OS requries: C, 58.04; H, 6.49; N, 22.56% 25 (c) A (830 mg) and 2-methylfuran-5-carboxaldehyde (0.3 mi) gave 1 -methyl- N5-[3-[3-[(2 methyl-5-fu ra nyl m ethyi)a m i no methyl] ph enoxyl propyll- 1 H-1,2,4triazole-3,5-dia mine which was isolated as its tartrate salt (1.15 g) m.p. softens ca. 951.

N.m.r. (D20) 2.53, t, (1 1-1); 2.8-3.0, m, (3 H); 3.46, d, (1 1-1); 3.87, cl, (1 1-1); 5.53, s, (2 H); 5.76, s, (2 1-1); 5.8, s, (2 H); 5.82, t, (2 H); 6.5, t, (2 W; 6.6, s, (3 H); 7.68, s, (3 H); 7.85, rn, (2 H). 30 (d) A (830 mg) and pyrrole-2-carboxaldehyde (286 mg) gave 1 -methyl-N5-[3[3-[(1 H-pyrrol-2-yi m ethyi)a m i no m ethyl] phenoxyl propyll- 1 H1,2,4-triazole-3,5-d lami ne which was isolated as its hydrated tartrate salt (420 mg).

N.m.r. (D20) 2.55, t, (1 H); 2.8-3.05, m, (4 1-1); 3.60, m, (2 H); 3.72, t, (1 H); 5.60, s, (2 1-1); 5.73, s, (2 H); 5.83, s+t, (4 H); 6.55, t, (2 H); 6.65, s, (3 H); 7.93, m, (2 H).

Assayfound: C2,H3,N.,07.H20 requires:

Example 3 N-Decyl-[3-[3-[(1 -methyl-3-amino-1 H-1,2,4-triazol-540 yi)a minol propoxy] phenyl methylamino]acetamide C, 50.99; H, 6.28; N, 18.74; C, 50.47; H, 6.35;N, 18.72% A mixture of methyl-[3-[340 -methyl-3-amino-1 H-1,2,4-triazol- 5yi)aminolpropoxylphenyimethylaminolacetate (110 mg) and decylamine (0.5 m]) was heated at 100' for 16 h. The crude reaction mixture was purified by preparative layer chromatography to give the title compound as a yellow oil (120 mg).

T.I.c. Rf 0.8.

N.m.r. (D20) 2.6-3.3, m, (5 H); 5.72, brA, (1 H); 5.93, t, (2 H); 6.18, br.s, (2 H); 6.30, s, (2 H); 6.47, q, (2 H); 6.65, s, (3 H); 6.72, s+m, (4 H); 7.88, m, (2 H); 8.0-8.9, m, (17 H); 9.15, br.t, (3 H).

Example 4 (a) 1 -M ethyl-5-[[3-[3- M2-fu ra nyl m ethyi)a m i no] m ethyl] phenoxyl propyll am inol- 1 H-1,2,4- 50 triazole-3-methanol A solution of 3-[3-[(3-hydroxym ethyl- 1 -methyl- 1,2,4-triazol-5-yi)a mi no] propoxyl benza Idehyde (871 mg) and furfurylamine (5.3 mi) in ethanol (30 mi) was stirred at room temperature for 6 h, then treated with sodium borohydride (1 g) for 17 h. The mixture was quenched with water, partially evaporated and extracted with ethyl acetate. The organic extract was evaporated and purified by column chromatography on silica using methanol/ethyl acetate (1:1) as eluant to give the title compound as a red oil (500 mg).

8 GB 2 063 253 A 8 T.I.c. system A Rf 0.7. The tartrate salt was formed in ethyl acetate m.p. softens at 701.

N.m.r. (D20) 2.35, d, (1 W; 2.55, dt, (1 H); 2.83-3.1, m, (3 H); 3.38, d, (1 H); 3.5, dd, (1 H); 5.56, s, (2 H); 5.6, s, (2 H); 5.7, s, (2 H); 5.8, te (2 H); 5.82, s, (2 H); 6.5, s+t, (5 H); 7.9, m, (2 H).

(b) Similarly, 3-[3-[(3-hydroxymethy]- 1 -methyl- 1,2,4-triazole-5yi)aminolpropoxylbenzaidehyde (790 mg) and dodecylamine (8 ml) gave 1methyi-5-[[3-[3[(dodecyi)aminomethyllphenoxylpropyllaminol-1 H,1,2,4triazole-3-methanol as a cream solid (550 mg) m.p. 55-57'.

N.m.r. (C1DC13) 2.76, t, (1 R 3.0-3.35, m, (3 R 5.48, s, (2 R 5.88, t, (2 H); 6.23, s, (2 H); 6.40, q, (2 W; 6.50, s, (3 H); 7.38, t, (2 H); 7.60, brs, (2 H); 7.88, q, (2 H); 8.3-8.9, m, (20 H); 9.13, m, (3 H).

Example 5 1 -Methy]-N 3-(4-pyridyl methylene)-N5-[3-[3-[(2-fu ranyl methyl) am inom ethyl) phenoxyl propyll-1 H1,2,4-triazole-3,5-diamine A solution of 1 -m ethyl-N5-[3-[3-[(2-fu ra nyl methyi)a m. inom ethyl] phenoxyl propyll- 1 H-1,2,4- triazole-3,5-diamine (310 mg) and 4-pyridinecarboxaldehyde (0.17 mi) in toluene (15 m]) was heated at reflux for 30 h. The solution was cooled to give the title compound as a yellow solid (250 mg) m.p. 90-940.

N.m.r. (C13C13) 0.92, s, (1 H); 1.25, m, (2 R 2.20, m, (2 H); 2.6-2.9, t+ m, (2 H); 3.0-3.3, m, (3 H); 3.7, m, (1 W; 3.85, m, (1 H); 5.4, t, (1 H); 5.84, t, (2 H); 6.1-6.5, m, (9 R 7.8, m, (2 R 8.2, s, (1 H).

Example 6 5-[[2-[[[5- UN-M ethyl (2-fura nyl methyi)a m i no] methyl]-2fura nyll methyl Ith iol ethyl] am inol-1 methyl-11 H-1,2,4-triazole-3methanol 5-[[2-[[[5-(methyla m ino) methyl-2-furanyll methyllthiol ethyl] amino]-11 -methyl-l H-1,2,4-triazole25 3-methanol A solution of 2-[[[5-(methylamino)methyi-2-furanyllmethyllthiolethanamine (4.0 g) and methyl N-[2-(acetoxy)acetyll-1-methyll-2-(phenyimetiiylene) hydrazine carboximidothioate (6.2 g) in acetonitrile (25 m]) was stirred for 3 h at 251C.

The solution was evaporated and the residue was chromatographed on silica with ethyl acetate/methanol (4:1) to give the title compound as a gum (3.5 g). T.I.c. silica methanol. Rf 0.45. A 30 portion of this material was converted to its oxalate salt m.p. 157-91.

5-[[2-[[[5-[[N-Methyi-(2-fura nyl methyi)a m inol methyl]-2-fura nyll methyllth iol ethyl] am i nol-11 - methy]-1 H-1,2,4-triazole-3-methanol A solution of 5-[[2-[[[5-(methylamino) methyi-2furanyllmethyllthiolethyllaminol- 1 -methyl- 1 H 1,2,4-triazole-3-methanol (0.5 g) and 2-furaldehyde (0.31 g) in ethanol (20 mO was stirred at 25 'C for 35 2 h then treated with sodium borohydride (38 mg). After 2 h 2-furaldehyde (0.31 9) was added and after a further 2 h sodium borohydride (76 mg) was added. The resulting solution was stirred for 18 h, treated with acetic acid (2 m]) and evaporated in vacuo. The residue was dissolved in water which was basified with potassium carbonate and extracted with ethyl acetate. The organic extracts were evaporated to give a gum which was chromatographed on silica with ethyl acetate:methanol (95:5) to 40 give the title compound as a gum (0.29 g).

T.I.c. silica; ethyl acetate:ethanol:O.88 ammonia (20:32); Rf 0.37.

N.m.r. (C13C13) 2.60, m, (1 H); 3.70, s+m, (4 H); 5.48, s+t, (3 H); 63-6. 6 4 s+m, (12 ffl; 7.20, t, (2 H); 7.7 5, s, (3 H).

Tablets

Active ingredient Microcrystal line Cellulose BPC Magnesium stearate BP Compression weight Examples of Pharmaceutical Compositions The active ingredient is sieved through a 250 Am sieve, blended with the excipients and compressed using 9.5 mm punches. Tablets of other strengths may be prepared by altering the compression weight and using punches to suit.

The tablets may be film coated with suitable film forming materials, e.g. methyl cellulose or hydroxypropyl methyl cellulose using standard techniques. Alternatively the tablets maybe sugar coated.

Injection for Intravenous Administration mgItablet

100.00 198.50 1.50 300.00 Active ingredient Water for injections B.P. to % W/V 1.00 100.00 9 GB 2 063 253 A 9 Sodium chloride may be added to adjust the tonicity of the solution and the pH may be adjusted to that of maximum stability and/or to facilitate solution of the active ingredient using either dilute acid or alkali.

The solution is prepared, clarified and filled into appropriate sized ampoules sealed by fusion of 5 the glass. The injection is sterilised by heating in an autoclave using one of the acceptable cycles. Alternatively the solution may be sterilised by filtration and filled into sterile ampoules under aseptic conditions. The solution may be packed under an inert atmosphere of nitrogen.

Claims

Claims

1. Compounds of the general formula (1) R3 1 N-N R1R2N-AIK-0-(CH2)n X (CH2) m NH--- N jL- R 4 (.1 10 and physiologically acceptable salts, hydrates and bioprecursors thereof, in which R, represents a heteroaralkyl group or a Cl l,, straight or branched saturated or unsaturated alkylene chain optionally substituted by a hydroxy group or R, represents a Cl-16 straight or branched saturated or unsaturated alkylene chain substituted by an ester group C02R. or an amide group CONH2. where R. is a C,-3 alkyl group, or R, represents a Cl- C,,, straight or branched saturated or unsaturated 15 alkylene chain interrupted by an oxygen atom, a sulphur atom, a sulphoxide group, a sulphone group, an amide group (-CONH- or -NHCO-) or an ester group (-CO-O- or -0-CO), with the provisos that Ri does not represent a Cl, alkyl group substituted by a Cl-, alkoxy group and that when the alkylene chain is interrupted by an oxygen or sulphur atom or a sulphoxide, amide (CONH) or ester - 20 (COO) group there must be at least two carbon atoms between that group and the nitrogen atom to which Ri is attached; R2 represents hydrogen or a Cl-4 alkyl group; Alk represents a straight or branched alkylene chain of 1 to 6 carbon atoms; Q represents a furan or thiophen ring in which incorporation into the rest of the molecule is 25 through bonds at 2- and 5-positions, the furan ring optionally bearing a further substituent R. adjacent to the group R1R2NAlk-, or Q represents a benzene ring in which incorporation into the rest of the molecule is through bonds at the 1 - and 3- or 1 - and 4-positions; R. represents halogen or Cl-4 alkyl which may be substituted by hydroxy or Cl-4 alkoxy; X represents -CH2-, -0-, -S- or -NH-; 30 n represents zero, 1 or 2; m represents 2, 3 or 4; R3 represents hydrogen, alkyl, alkenyl, aralkyl, C2-, alkyl substituted by hydroxy or alkoxy; and R4 represents hydrogen, alkyl, alkenyl, aralkyl, hydroxyalkyl, acyloxyalky], alkoxyalkyi, aryloxyalkyl, aralkyloxyalkyl, aminoalkyl, alkylaminoalkyl, dialkylaminoalkyl, hydroxy or alkoxy or the group NRA, 35 where R7 represents hydrogen, alkyl, alkyl substituted by hydroxy or Cl-, alkoxy, alkenyl, aralkyl or heteroaralkyl and R. represents any of the groups defined for R7 or may represent the group C0% where R. represents hydrogen, alkyl, aryl, aralkyl, alkoxy, heteroaryl or monocyclic heteroaralkyl or R.

represents the group SO2131. where RIO represents alkyl or aryl, or R. represents the group C-NHR,l 11 Y where Y is oxygen or sulphur, and R,, represents hydrogen, alky], cycloalky], aryl or aralkyl, or R7 and R. taken together represent the group =CRUR13 where R12 represents aryl or heteroary] 4,5 and Ri. represents hydrogen or alkyl; with the proviso that when Xis -NH- then n is zero.

2. Compounds as claimed in Claim 1 in which the groups RJ, R2, R3 and R4 have the following meanings:

Ri: a heteroaralkyl group in which the heterocyclic portion is a furyl, thienyl, pyrrolyi, pyridinyl, pyrimidiny], triaziny], oxazolyl, triazolyl, thiazolyl, isoquinolinyi, quinolinyl or indolyl ring and the alkylene portion is a methylene, ethylene or propylene grouping; or an alkylene chain which is saturated and 50 contains up to 16 carbon atoms or is substituted by or interrupted by an amide or ester grouping; R2: hydrogen or a methyl or ethyl group; R3: hydrogen, C,-4 alky], or hydroxy C2-4 alkyl; R4: hydrogen, hydroxy, Cl-4 alkyl, hydroxy Cl-4 alkyl, Cl-3 alkoxy Cl-4 alkyl, phenyl Cl-3 alkyl, C2-4 GB 2 063 253 A 10 alkanoyloxy C,-4 alkyl, amino Cl-4 alkyl, amino, Cl-4 alkylamino or di-Cl- 4 alkylamino, phenyl Cl-, alkylamino, or a heteroaryl Cl-3 alkylamino group where the heteroaryl ring is 5 or 6 membered and contains one heteroatom; or the group NHCOR, where R. represents hydrogen, Cl-3 alkyl, Cl-3 alkoxy, furyl, pyridyi, thiazolyl, thienyl, or phenyl optionally substituted by a C 1-, alkyl or Cl-3 alkoxy group; or the group NHCONHR1, where Ril is Cl-, alkyl, C.-7 cycloalkyl or phenyl optionally substituted by a Cl-3 alkyl or C,3 alkoxy group; or the group N=CHR12 where R12 is a phenyl or pyridyl group; the group Alk is (CHJP where p is 1, 2 or 3; and the group Q is a benzene ring incorporated into the rest of the molecule through bonds at the 1 - and 3- or 1 - and 4-positions, with n as zero, X as oxygen and m as 3 or 4; or the group Q is a furan or thiophene ring with n as 1, X as sulphur and m as 2, with the furan ring optionally containing a 10 further substituent R. where R. is bromine, C,-3 alkyl or Cl-, alkoxy-Cl- 3 alkyl; or the group Q is a furan ring with n as 1, X as CH2 and m as 2.

3. Compounds as claimed in Claim 2 in which the groups Rj, R21 R3 and R4 have the following meanings:

RI: a heteroaryl methyl group where the heteraryl ring is 5- or 6membered, contains one 15 heteroatom and is linked to the methyl portion via a carbon atom; or a Cli1. straight or branched saturated alkylene chain; or a Cl-,, straight or branched saturated alkylene chain interrupted by an amide (-NHCO-) group; R2: hydrogen or a methyl or ethyl group; R3: hydrogen, methyl, ethyl or 2-hydroxyethyl; R4: hydroxy, phenyl C,-3 alkyl, C,-4 alkyl substituted by hydroxy, C,3 alkoxy, C2-4 alkanoyloxy or amino; amino, di-Cl-4 alkylamino, NHCOR, where R. represents hydrogen, Cl- 3 alkoxy or phenyl, NHCONHR11 where R,, represents phenyl, or N=CHR12 where R,,. is phenyl or pyridyi; the group Alk is a methylene or ethylene group; and the group Q is a benzene ring incorporated into the rest of the molecule through bonds at the 1 - 25 and 3- or 1 - and 4-positions, with n as zero, X as oxygen and m as 3 or 4; or the group Q is a furan or thiophene ring with n as 1, X as sulphur and m as 2, with the furan ring optionally containing a further substituent R. where R. is bromine, Cl-3 alkyl or C,-, alkoxy-Cl_. alky]; or the group Q is a furan ring with n as 1, X as CH2 and m as 2.

4. Compounds as claimed in any of Claims 1 to 3 in which the group Q is a benzene ring incorporated into the rest of the molecule through bonds at the 1 - and 3- positions, with n as zero, X as oxygen and m as 3 or 4; or the group Q is a furan ring optionally containing a further substituent R.

where R. is C,-4 alkyl, with n as 1, X as sulphur and m as 2.

5. Compounds as claimed in any of Claims 1 to 4 in which R, is a heteroaralkyl group.

6. Compounds as claimed in Claim 5, corresponding to formula (11) R3 R 14 CH2 "Z 1 1 N-N NCH2--(: 0 (CH 2) 3NH V 4 (E) P2 N where R,4 is a 5- or 6-membered heteroaryl group containing one heteroatom and linked through a carbon atom, R2 is hydrogen or methyl; R, 3 is hydrogen or methyl; and R4 is an amino, hydroxyalkyl, alkanoyloxyalkyl, alkoxyalkyl, aminoalkyl, benzyi, formamido, alkoxycarbonylamino, alkanoylamino, 40 hydroxy, aroylamino, phenylcarbamoylamino, orpyridyimethylenamino group.

7. Compounds as claimed in Claim 1 which are 1 -methyl-N5-[3-[3[(dodecyla m ino) methyl] phenoxyl propyll 1 H-1,2,4-triazole-3,5-diamine, N-butyi-[3-[3-[(1-methyi-3-amino-1H-1,2,4-triazo1-5-yi)amino] r)ror)oxvlnhenvimethviaminolhexana mide 1 1 -m ethyl-N543- [3-[(24ura nyl methyi)a minom ethyl] ph enoxyl propyll 1 H- 1,2,4-triazole-3,5diamine, 1 -methyl-N5-[3-[3-[(1 H-pyrrol-2-yi-methyi)aminomethyllphenoxylpropyll-1 H-1,2,4-triazole-3,5 diamine, 5-[[2-[[[5-[[N-methyi-(2-furanyimethyi)am inol methyl]-2-furanyll methyllthiolethyll amino]- 1 - methyM H-1,2,4-triazole-3-methanol, and their physiologically acceptable salts.

8. Compounds as claimed in Claim 1 in which Ri represents a heteroaralkyl group; and R4 represents NR7R. where R. and R. each represent hydrogen, alkyl, alkyl substituted by hydroxy or Cl-3 alkoxy, alkenyi, aralkyl or heteroaralkyl or R7 and R. together represent =CR121113 where R12 55 represents aryl or heteroaryl and R13 represents hydrogen or alkyl.

9. A process for the production of compounds of formula (1) as defined in Claim 1 which comprises:

11 GB 2 063 253 A 11 (a) for the production of compounds in which Alk is CH2 and R4 is other than -N=CR 12R131 reducing the intermediate formed by reacting a compound of formula (111) R 3 1 N-N (IE) W-0 (C H 2), X ( C H 2) m N -R 4 in which W represents the group -CHO, with a reagent of formula R1R2NH; (b) for the production of compounds in which R4 is other than -N=CRUR,., reacting a compound 5 of formula OV) R 13 N-N R2MAIKO (CH2), X (CH2)rn NH R 4 (EZ) with an aldehyde RICHO under reducing conditions, where the group R] has a meaning such that the resulting group R11C1-12- represents a group R,; (c) reacting a compound of formula (IV) as defined above with a compound R, Hal where Hal is 10 halogen; (c) for the production of compounds in which R 4 represents N=CRUIR1, reacting a compound of formula (1) in which R4 is NI-12 with an aldehyde or ketone RJ1,C0; (e) for the production of compounds in which R4 represents an acyloxyalkyl group, reacting a compound in which R4 represents hydroxyalkyl with an appropriate acid; (f) for the production of compounds in which R4 is the group NR7R. where R. is -COR., -S02Rj. or-C(=Y)NHR11, treating an aminotriazole (V) R 3 1 N-N R1 R2NAI K-0-(CH 2) n X (CH 2) m NH NHIR7 (V) in which Rj, R2, R, and R, are as defined in formula (1) or are groups readily convertible thereto, with a reagent capable of replacing the hydrogen atom in the group NHR7 by the group IR,, where R. is as 20 defined above; (g) for the production of compounds in which R1 is an alkylene chain interrupted by an amide grouping, treating a compound of formula (1) in which R, is an alkylene chain substituted by an ester grouping CO.R. (i.e. R, is the group R.0OC(CH AC_) with an amine CH,(CH, )YM2 to give a compound of formula (1) in which R1 is the group CH,(C1-12), NHCO(CH,),-- where x and y are integers and the total 25 of x and y is not greater than 15; and where the compound of formula (1) is produced in the form of a free base, optionally converting the free base into a salt.

10. A pharmaceutical composition comprising a compound as claimed in Claims 1 to 8 together with at least one pharmaceutical ly acceptable carrier or diluent, and optionally one or more further active ingredients.

11. Compounds as claimed in Claim 6, in which R4 represents amino, hydroxyalkyl or pyridyimethylenamino.

Printed for Her Majesty's Stationery Office by the Courier Press, Leamington Spa, 1981. Published by the Patent Office, 25 Southampton Buildings, London, WC2A 'I AY, from which copies maybe obtained.